Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,120,216 C→A 83.4% D389Y (GAT→TAT)  glcD ← glycolate oxidase subunit, FAD‑linked

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,120,2160CA83.4% 591.4 / 131.3 253D389Y (GAT→TAT) glcDglycolate oxidase subunit, FAD‑linked
Reads supporting (aligned to +/- strand):  ref base C (12/30);  new base A (108/103);  total (120/133)
Fisher's exact test for biased strand distribution p-value = 1.05e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.69e-01

Only 100 of 253 total aligned reads displayed.

TTTTTCTCGCCCGATGCCATGTTCGCCACTGATGCTGCCGCCAACTTCAACGCAGAGTTCGAGGATCTTCCCGCCCAGCTCTTCCGCGCGGGCAAATTCACCGGGTTCGTTGGCATCGAAAAGGATTAACGGGTGCATGTTGCCATCTCCGGCATGAAAGACGTTGGCAACACGTAAATCATATTGCTGCGATAAACGGGCAATGCCTTCCAGTACGCCAGGCAGGGCGCGACGCGGGATGGTGCCATCCATGCAGTAGTAATCCGGGGAGATACGTCCTACCGCCGGGAACG  >  CP009273/3120070‑3120362
                                                                                                                                                  |                                                                                                                                                  
tttttCTCGCCCGATGCCATGTTCGCCACTGATGCTGCCGCCAACTTCAACGCAGAGTTCGAGGATCTTCCCGCCCAGCTCTTCCGCGCGGGCAAATTCACCGGGTTCGTTGGCATCGAAAAGGATTAACGGGTGCATGTTGCCATCTcc                                                                                                                                                 <  2:4275609/150‑1 (MQ=255)
    tctcGCCCGATGCCATGTTCGCCACTGATGCTGCCGCCAACTTCAACGCAGAGTTCGAGGATCTTCCCGCCCAGCTCTTCCGCGCGGGCAAATTCACCGGGTTCGTTGGCATCGAAAAGGATTAACGGGTGCATGTTGCCATCTCCGGCa                                                                                                                                             >  2:1561595/1‑150 (MQ=255)
    tctcGCCCGATGCCATGTTCGCCACTGATGCTGCCGCCAACTTCAACGCAGAGTTCGAGGATCTTCCCGCCCAGCTCTTCCGCGCGGGCAAATTCACCGGGTTCGTTGGCATCGAAAAGGATTAACGGGTGCATGTTGCCATATCCGGCa                                                                                                                                             <  1:1300579/150‑1 (MQ=255)
      tcGCCCGATGCCATGTTCGCCACTGATGCTGCCGCCAACTTCAACGCAGAGTTCGAGGATCTTCCCGCCCAGCTCTTCCGCGCGGGCAAATTCACCGGGTTCGTTGGCATCGAAAAGGATTAACGGGTGCATGTTGCCATATCCGGCATg                                                                                                                                           <  2:3863073/150‑1 (MQ=255)
      tcGCCCGATGCCATGTTCGCCACTGATGCTGCCGCCAACTTCAACGCAGAGTTCGAGGATCTTCCCGCCCAGCTCTTCCGCGCGGGCAAATTCACCGGGTTCGTTGGCATCGAAAAGGATTAACGGGTGCATGTTGCCATATCCGGCATg                                                                                                                                           <  2:358795/150‑1 (MQ=255)
      tcGCCCGATGCCATGTTCGCCACTGATACTGCCGCCAACTTCAACGCAGAGTTCGAGGATCTTCCCGCCCAGCTCTTCCGCGCGGGCAAATTCACCGGGTTCGTTGGCATCGAAAAGGATTAACGGGTGCATGTTGCCATATCCGGCATg                                                                                                                                           >  1:554772/1‑150 (MQ=255)
        gCCCGATGCCATGTTCGCCACTGATGCTGCCGCCAACTTCAACGCAGAGTTCGAGGATCTTCCCGCCCAGCTCTTCCGCGCGGGCAAATTCACCGGGTTCGTTGGCATCGAAAAGGATTAACGGGTGCATGTTGCCATATCCGGCATGaa                                                                                                                                         >  1:101726/1‑150 (MQ=255)
         cccGATGCCATGTTCGCCACTGATGCTGCCGCCAACTTCAACGCAGAGTTCGAGGATCTTCCCGCCCAGCTCTTCCGCGCGGGCAAATTCACCGGGTTCGTTGGCATCGAAAAGGATTAACGGGTGCATGTTGCCATCTCCGGCATGaaa                                                                                                                                        <  2:4184579/150‑1 (MQ=255)
           cGATGCCATGTTCGCCACTGATGCTGCCGCCAACTTCAACGCAGAGTTCGAGGATCTTCCCGCCCAGCTCTTCCGCGCGGGCAAATTCACCGGGTTCGTTGGCATCGAAAAGGATTAACGGGTGCATGTTGCCATATCCGGCATGAAAGa                                                                                                                                      >  1:3886426/1‑150 (MQ=255)
           cGATGCCATGTTCGCCACTGATGCTGCCGCCAACTTCAACGCAGAGTTCGAGGATCTTCCCGCCCAGCTCTTCCGCGCGGGCAAATTCACCGGGTTCGTTGGCATCGAAAAGGATTAACGGGTGCATGTTGCCATATCCGGCATGAAAGa                                                                                                                                      >  1:3399284/1‑150 (MQ=255)
              tGCCATGTTCGCCACTGATGCTGCCGCCAACTTCAACGCAGAGTTCGAGGATCTTCCCGCCCAGCTCTTCCGCGCGGGCAAATTCACCGGGTTCGTTGGCATCGAAAAGGATTAACGGGTGCATGTTGCCATATCCGGCATGAAAGACGt                                                                                                                                   >  1:302488/1‑150 (MQ=255)
               gCCATGTTCGCCACTGATGCTGCCGCCAACTTCAACGCAGAGTTCGAGGATCTTCCCGCCCAGCTCTTCCGCGCGGGCAAATTCACCGGGTTCGTTGGCATCGAAAAGGATTAACGGGTGCATGTTGCCATCTCCGGCATGAAAGACGtt                                                                                                                                  >  1:1505714/1‑150 (MQ=255)
                ccATGTTCGCCACTGATGCTGCCGCCAACTTCAACGCAGAGTTCGAGGATCTTCCCGCCCAGCTCTTCCGCGCGGGCAAATTCACCGGGTTCGTTGGCATCGAAAAGGATTAACGGGTGCATGTTGCCATATCCGGCATGAAAGACGTTg                                                                                                                                 >  2:891135/1‑150 (MQ=255)
                   tGTTCGCCACTGATGCTGCCGCCAACTTCAACGCAGAGTTCGAGGATCTTCCCGCCCAGCTCTTCCGCGCGGGCAAATTCACCGGGTTCGTTGGCATCGAAAAGGATTAACGGGTGCATGTTGCCATATCCGGCATGAAAGACGTTGGCa                                                                                                                              >  1:990875/1‑150 (MQ=255)
                    gTTCGCCACTGATGCTGCCGCCAACTTCAACGCAGAGTTCGAGGATCTTCCCGCCCAGCTCTTCCGCGCGGGCAAATTCACCGGGTTCGTTGGCATCGAAAAGGATTAACGGGTGTATGTTGCCATATCCGGCATGAAAGACGTTGGCaa                                                                                                                             <  2:2536687/150‑1 (MQ=255)
                       cGCCACTGATGCTGCCGCCAACTTCAACGCAGAGTTCGAGGATCTTCCCGCCCAGCTCTTCCGCGCGGGCAAATTCACCGGGTTCGTTGGCATCGAAAAGGATTAACGGGTGCATGTTGCCATATCCGGCATGAAAGACGTTGGCAacac                                                                                                                          <  2:185293/150‑1 (MQ=255)
                       cGCCACTGATGCTGCCGCCAACTTCAACGCAGAGTTCGAGGATCTTCCCGCCCAGCTCTTCCGCGCGGGCAAATTCACCGGGTTCGTTGGCATCGAAAAGGATTAACGGGTGCATGTTGCCATATCCGGCATGAAAGACGTTGGCAacac                                                                                                                          >  1:4385592/1‑150 (MQ=255)
                          cACTGATGCTGCCGCCAACTTCAACGCAGAGTTCGAGGATCTTCCCGCCCAGCTCTTCCGCGCGGGCAAATTCACCGGGTTCGTTGGCATCGAAAAGGATTAACGGGTGCATGTTGCCATATCCGGCATGAAAGACGTTGGCAACACGTa                                                                                                                       >  1:732155/1‑150 (MQ=255)
                                 gcttcCGCCAACTTCAACGCAGAGTTCGAGGATCTTCCCGCCCAGCTCTTCCGCGCGGGCAAATTCACCGGGTTCGTTGGCATCGAAAAGGATTAACGGGTGCATGTTGCCATCTCCGGCATGAAAGACGTTGGCAACACGTAAATCata                                                                                                                <  2:3252106/150‑1 (MQ=255)
                                       gccAACTTCAACGCAGAGTTCGAGGATCTTCCCGCCCAGCTCTTCCGCGCGGGCAAATTCACCGGGTTCGTTGGCATCGAAAAGGATTAACGGGTGCATGTTGCCATATCCGGCATGAAAGACGTTGGCAACACGTAAATCATATtgctg                                                                                                          <  2:1917601/150‑1 (MQ=255)
                                       gccAACGTCAACGCAGAGTTCGAGGATCTTCCCGCCCAGCTCTTCCGCGCGGGCAAATTCACCGGGTTCGTTGGCATCGAAAAGGATTAACGGGTGCATGTTGCCATATCCGGCATGAAAGACGTTGGCAACACGTAAATCATATtgctg                                                                                                          <  2:3391749/150‑1 (MQ=255)
                                        ccAACTTCAACGCAGAGTTCGAGGATCTTCCCGCCCAGCTCTTCCGCGCGGGCAAATTCACCGGGTTCGTTGGCATCGAAAAGGATTAACGGGTGCATGTTGCCATATCCGGCATGAAAGACGTTGGCAACACGTAAATCATATtgctgc                                                                                                         >  1:4054217/1‑150 (MQ=255)
                                         cAACTTCAACGCAGAGTTCGAGGATCTTCCCGCCCAGCTCTTCCGCGCGGGCAAATTCACCGGGTTCGTTGGCATCGAAAAGGATTAACGGGTGCATGTTGCCATATCCGGCATGAAAGACGTTGGCAACACGTAAATCATATTGCTGCg                                                                                                        <  1:957819/150‑1 (MQ=255)
                                         cAACTTCAACGCAGAGTTCGAGGATCTTCCCGCCCAGCTCTTCCGCGCGGGCAAATTCACCGGGTTCGTTGGCATCGAAAAGGATTAACGGGTGCATGTTGCCATATCCGGCATGAAAGACGTTGGCAACACGTAAATCATATTGCTGCg                                                                                                        <  1:3541063/150‑1 (MQ=255)
                                            cTTCAACGCAGAGTTCGAGGATCTTCCCGCCCAGCTCTTCCGCGCGGGCAAATTCACCGGGTTCGTTGGCATCGAAAAGGATTAACGGGTGCATGTTGCCATATCCGGCATGAAAGACGTTGGCAACACGTAAATCATATTGCTGCGATa                                                                                                     >  1:1892124/1‑150 (MQ=255)
                                            cTTCAACGCAGAGTTCGAGGATCTTCCCGCCCAGCTCTTCCGCGCGGGCAAATTCACCGGGTTCGTTGGCATCGAAAAGGATTAACGGGTGCATGTTGCCATATCCGGCATGAAAGACGTTGGCAACACGTAAATCATATTGCTGCGATa                                                                                                     <  2:2945129/150‑1 (MQ=255)
                                               cAACGCAGAGTTCGAGGATCTTCCCGCCCAGCTCTTCCGCGCGGGCAAATTCACCGGGTTCGTTGGCATCGAAAAGGATTAACGGGTGCATGTTGCCATATCCGGCATGAAAGACGTTGGCAACACGTAAATCATATTGCTGCGATAAAc                                                                                                  >  2:2295857/1‑150 (MQ=255)
                                                aaCGCAGAGTTCGAGGATCTTCCCGCCCAGCTCTTCCGCGCGGGCAAATTCACCGGGTTCGTTGGCATCGAAAAGGATTAACGGGTGCATGTTGCCATCTCCGGCATGAAAGACGTTGGCAACACGTAAATCATATTGCTGCGATAAACg                                                                                                 <  1:523312/150‑1 (MQ=255)
                                                aaCGCAGAGTTCGAGGATCTTCCCGCCCAGCTCTTCCGCGCGGGCAAATTCACCGGGTTCGTTGGCATCGAAAAGGATTAACGGGTGCATGTTGCCATATCCGGCATGAAAGACGTTGGCAACACGTAAATCATATTGCTGCGATAAACg                                                                                                 >  2:627892/1‑150 (MQ=255)
                                                  cGCAGAGTTCGAGGATCTTCCCGCCCAGCTCTTCCGCGCGGGCAAATTCACCGGGTTCGTTGGCATCGAAAAGGATTAACGGGTGCATGTTGCCATATCCGGCATGAAAGACGTTGGCAACACGTAAATCATATTGCTGCGATAAACggg                                                                                               <  2:2975244/150‑1 (MQ=255)
                                                          tCGAGGATCTTCCCGCCCAGCTCTTCCGCGCGGGCAAATTCACCGGGTTCGTTGGCATCGAAAAGGATTAACGGGTGCATGTTGCCATATCCGGCATGAAAGACGTTGGCAACACGTAAATCATATTGCTGCGATAAACGGGCAATGCCt                                                                                       >  2:2356842/1‑150 (MQ=255)
                                                           cGAGGATCTTCCCGCCCAGCTCTTCCGCGCGGGCAAATTCACCGGGTTCGTTGGCATCGAAAAGGATTAACGGGTGCATGTTGCCATATCCGGCATGAAAGACGTTGGCAACACGTAAATCATATTGCTGCGATAAACGGGCAATGCCtt                                                                                      <  2:2066981/150‑1 (MQ=255)
                                                            gAGGATCTTCCCGCCCAGCTCTTCCGCGCGGGCAAATTCACCGGGTTCGTTGGCATCGAAAAGGATTAACGGGTGCATGTTGCCATCTCCGGCATGAAAGACGTTGGCAACACGTAAATCATATTGCTGCGATAAACGGGCAATGCCTTc                                                                                     <  1:2516061/150‑1 (MQ=255)
                                                            gAGGATCTTCCCGCCCAGCTCTTCCGCGCGGGCAAATTCACCGGGTTCGTTGGCATCGAAAAGGATTAACGGGTGCATGTTGCCATATCCGGCATGAAAGACGTTGGCAACACGTAAATCATATTGCTGCGATAAACGGGCAATGCCTTc                                                                                     >  2:4142021/1‑150 (MQ=255)
                                                             aGGATCTTCCCGCCCAGCTCTTCCGCGCGGGCAAATTCACCGGGTTCGTTGGCATCGAAAAGGATTAACGGGTGCATGTTGCCATCTCCGGCATGAAAGACGTTGGCAACACGTAAATCATATTGCTGCGATAAACGGGCAATGCCTTcc                                                                                    <  2:1563133/150‑1 (MQ=255)
                                                             aGGATCTTCCCGCCCAGCTCTTCCGCGCGGGCAAATTCACCGGGTTCGTTGGCATCGAAAAGGATTAACGGGTGCATGTTGCCATATCCGGCATGAAAGACGTTGGCAACACGTAAATCATATTGCTGCGATAAACGGGCAATGCCTTcc                                                                                    <  2:1440672/150‑1 (MQ=255)
                                                               gATCTTCCCGCCCAGCTCTTCCGCGCGGGCAAATTCACCGGGTTCGTTGGCATCGAAAAGGATTAACGGGTGCATGTTGCCATCTCCGGCATGAAAGACGTTGGCAACACGTAAATCATATTGCTGCGATAAACGGGCAATGCCTTCCAg                                                                                  <  1:1465623/150‑1 (MQ=255)
                                                                   ttCCCGCCCAGCTCTTCCGCGCGGGCAAATTCACCGGGTTCGTTGGCATCGAAAAGGATTAACGGGTGCATGTTGCCATATCCGGCATGAAAGACGTTGGCAACACGTAAATCATATTGCTGCGATAAACGGGCAATGCCTTCCAGTACg                                                                              >  2:2402631/1‑150 (MQ=255)
                                                                    tCCCGCCCAGCTCTTCCGCGCGGGCAAATTCACCGGGTTCGTTGGCATCGAAAAGGATTAACGGGTGCATGTTGCCATATCCGGCATGAAAGACGTTGGCAACACGTAAATCATATTGCTGCGATAAACGGGCAATGCCTTCCAGTACGc                                                                             >  1:2021865/1‑150 (MQ=255)
                                                                        gCCCAGCTCTTCCGCGCGGGCAAATTCACCGGGTTCGTTGGCATCGAAAAGGATTAACGGGTGCATGTTGCCATATCCGGCATGAAAGACGTTGGCAACACGTAAATCATATTGCTGCGATAAACGGGCAATGCCTTCCAGTACGCcagg                                                                         <  2:515399/150‑1 (MQ=255)
                                                                         cccAGCTCTTCCGCGCGGGCAAATTCACCGGGTTCGTTGGCATCGAAAAGGATTAACGGGTGCATGTTGCCATATCCGGCATGAAAGACGTTGGCAACACGTAAATCATATTGCTGCGATAAACGGGCAATGCCTTCCAGTACGCcaggc                                                                        >  2:3771374/1‑150 (MQ=255)
                                                                         cccAGCTCTTCCGCGCGGGCAAATTCACCGGGTTCGTTGGCATCGAAAAGGATTAACGGGTGCATGTTGCCATATCCGGCATGAAAGACGTTGGCAACACGTAAATCATATTGCTGCGATAAACGGGCAATGCCTTCCAGTACGCcaggc                                                                        >  1:2377343/1‑150 (MQ=255)
                                                                          ccAGCTCTTCCGCGCGGGCAAATTCACCGGGTTCGTTGGCATCGAAAAGGATTAACGGGTGCATGTTGCCATATCCGGCATGAAAGACGTTGGCAACACGTAAATCATATTGCTGCGATAAACGGGCAATGCCTTCCAGTACGCcaggca                                                                       >  2:3461675/1‑150 (MQ=255)
                                                                          ccAGCTCTTCCGCGCGGGCAAATTCACCGGGTTCGTTGGCATCGAAAAGGATTAACGGGTGCATGTTGCCATATCCGGCATGAAAGACGTTGGCAACACGTAAATCATATTGCTGCGATAAACGGGCAATGCCTTCCAGTACGCcaggca                                                                       >  2:120341/1‑150 (MQ=255)
                                                                          ccAGCTCTTCCGCGCGGGCAAATTCACCGGGTTCGTTGGCATCGAAAAGGATTAACGGGTGCATGTTGCCATATCCGGCATGAAAGACGTTGGCAACACGTAAATCATATTGCTGCGATAAACGGGCAATGCCTTCCAGTACGCcaggca                                                                       <  2:1304085/150‑1 (MQ=255)
                                                                          ccAGCTCTTCCGCGCGGGCAAATTCACCGGGTTCGTTGGCATCGAAAAGGATTAACGGGTGCATGTTGCCATATCCGGCATGAAAGACGTTGGCAACACGTAAATCATATTGCTGCGATAAACGGGCAATGCCTTCCAGTACGCcaggca                                                                       >  2:2165381/1‑150 (MQ=255)
                                                                          ccAGCTCTTCCGCGCGGGCAAATTCACCGGGTTCGTTGGCATCGAAAAGGATTAACGGGTGCATGTTGCCATATCCGGCATGAAAGACGTTGGCAACACGTAAATCATATTGCTGCGATAAACGGGCAATGCCTTCCAGTACGCcaggca                                                                       >  2:2388234/1‑150 (MQ=255)
                                                                           cAGCTCTTCCGCGCGGGCAAATTCACCGGGTTCGTTGGCATCGAAAAGGATTAACGGGTGCATGTTGCCATCTCCGGCATGAAAGACGTTGGCAACACGTAAATCATATTGCTGCGATAAACGGGCAATGCCTTCCAGTACGCcaggcag                                                                      <  2:2853505/150‑1 (MQ=255)
                                                                            aGCTCTTCCGCGCGGGCAAATTCACCGGGTTCGTTGGCATCGAAAAGGATTAACGGGTGCATGTTGCCATATCCGGCATGAAAGACGTTGGCAACACGTAAATCATATTGCTGCGATAAACGGGCAATGCCTTCCAGTACGCcaggcagg                                                                     <  1:3977647/150‑1 (MQ=255)
                                                                                   ccGCGCGGGCAAATTCACCGGGTTCGTTGGCATCGAAAAGGATTAACGGGTGCATGTTGCCATATCCGGCATGAAAGACGTTGGCAACACGTAAATCATATTGCTGCGATAAACGGGCAATGCCTTCCAGTACGCCAGGCAGGGCGCGAc                                                              <  1:4438503/150‑1 (MQ=255)
                                                                                   ccGCGCGGGCAAATTCACCGGGTTCGTTGGCATCGAAAAGGATTAACGGGTGCATGTTGCCATATCCGGCATGAAAGACGTTGGCAACACGTAAATCATATTGCTGCGATAAACGGGCAATGCCTTCCAGTACGCCAGGCAGGGCGCGAc                                                              >  2:3432931/1‑150 (MQ=255)
                                                                                        cgGGCAAATTCACCGGGTTCGTTGGCATCGAAAAGGATTAACGGGTGCATGTTGCCATCTCCGGCATGAAAGACGTTGGCAACACGTAAATCATATTGCTGCGATAAACGGGCAATGCCTTCCAGTACGCCAGGCAGGGCGCGACGCggg                                                         <  2:2019082/150‑1 (MQ=255)
                                                                                        cgGGCAAATTCACCGGGTTCGTTGGCATCGAAAAGGATTAACGGGTGCATGTTGCCATATCCGGCATGAAAGACGTTGGCAACACGTAAATCATATTGCTGCGATAAACGGGCAATGCCTTCCAGTACGCCAGGCAGGGCGCGACGCggg                                                         >  1:875486/1‑150 (MQ=255)
                                                                                         gggCAAATTCACCGGGTTCGTTGGCATCGAAAAGGATTAACGGGTGCATGTTGCCATATCCGGCATGAAAGACGTTGGCAACACGTAAATCATATTGCTGCGATAAACGGGCAATGCCTTCCAGTACGCCAGGCAGGGCGCGACGCGGGa                                                        <  2:3306593/150‑1 (MQ=255)
                                                                                         gggCAAATTCACCGGGTTCGTTGGCATCGAAAAGGATTAACGGGTGCATGTTGCCATATCCGGCATGAAAGACGTTGGCAACACGTAAATCATATTGCTGCGATAAACGGGCAATGCCTTCCAGTACGCCAGGCAGGGCGCGACGCGGGa                                                        <  1:2283848/150‑1 (MQ=255)
                                                                                           gCAAATTCACCGGGTTCGTTGGCATCGAAAAGGATTAACGGGTGCATGTTGCCATATCCGGCATGAAAGACGTTGGCAACACGTAAATCATATTGCTGCGATAAACGGGCAATGCCTTCCAGTACGCCAGGCAGGGCGCGACGCGGGATg                                                      <  1:120108/150‑1 (MQ=255)
                                                                                            cAAATTCACCGGGTTCGTTGGCATCGAAAAGGATTAACGGGTGCATGTTGCCATATCCGGCATGAAAGACGTTGGCACCACGTAAATCATATTGCTGCGATAAACGGGCAATGCCTTCCAGTACGCCAGGCAGGGCGCGACGCGGGATgg                                                     >  2:4493502/1‑150 (MQ=255)
                                                                                             aaaTTCACCGGGTTCGTTGGCATCGAAAAGGATTAACGGGTGCATGTTGCCATATCCGGCATGAAAGACGTTGGCAACACGTAAATCATATTGCTGCGATAAACGGGCAATGCCTTCCAGTACGCCAGGCAGGGCGCGACGCGGGATGGt                                                    <  2:3413491/150‑1 (MQ=255)
                                                                                             aaaTTCACCGGGTTCGTTGGCATCGAAAAGGATTAACGGGTGCATGTTGCCATATCCGGCATGAAAGACGTTGGCAACACGTAAATCATATTGCTGCGATAAACGGGCAATGCCTTCCAGTACGCCAGGCAGGGCGCGACGCGGGATGGt                                                    <  1:3832362/150‑1 (MQ=255)
                                                                                             aaaTTCACCGGGTTCGTTGGCATCGAAAAGGATTAACGGGTGCATGTTGCCATATCCGGCATGAAAGACGTTGGCAACACGTAAATCATATTGCTGCGATAAACGGGCAATGCCTTCCAGTACGCCAGGCAGGGCGCGACGCGGGATGGt                                                    >  2:834560/1‑150 (MQ=255)
                                                                                               aTTCACCGGGTTCGTTGGCATCGAAAAGGATTAACGGGTGCATGTTGCCATATCCGGCATGAAAGACGTTGGCAACACGTAAATCATATTGCTGCGATAAACGGGCAATGCCTTCCAGTACGCCAGGCAGGGCGCGACGCGGGATGGTGc                                                  >  1:3275837/1‑150 (MQ=255)
                                                                                                 tCACCGGGTTCGTTGGCATCGAAAAGGATTAACGGGTGCATGTTGCCATATCCGGCATGAAAGACGTTGGCAACACGTAAATCATATTGCTGCGATAAACGGGCAATGCCTTCCAGTACGCCAGGCAGGGCGCGACGCGGGATGGTGcca                                                >  2:3692215/1‑150 (MQ=255)
                                                                                                 tCACCGGGTTCGTTGGCATCGAAAAGGATTAACGGGTGCATGTTGCCATATCCGGCATGAAAGACGTTGGCAACACGTAAATCATATTGCTGCGATAAACGGGCAATGCCTTCCAGTACGCCAGGCAGGGCGCGACGCGGGATGGTGcca                                                >  2:816221/1‑150 (MQ=255)
                                                                                                   aCCGGGTTCGTTGGCATCGAAAAGGATTAACGGGTGCATGTTGCCATATCCGGCATGAAAGACGTTGGCAACACGTAAATCATATTGCTGCGATAAACGGGCAATGCCTTCCAGTACGCCAGGCAGGGCGCGACGCGGGATGGTGccatc                                              <  2:599518/150‑1 (MQ=255)
                                                                                                     cGGGTTCGTTGGCATCGAAAAGGATTAACGGGTGCATGTTGCCATATCCGGCATGAAAGACGTTGGCAACACGTAAATCATATTGCTGCGATAAACGGGCAATGCCTTCCAGTACGCCAGGCAGGGCGCGACGCGGGATGGTGccatcca                                            >  1:2013874/1‑150 (MQ=255)
                                                                                                      gggTTCGTTGGCATCGAAAAGGATTAACGGGTGCATGTTGCCATATCCGGCATGAAAGACGTTGGCAACACGTAAATCATATTGCTGCGATAAACGGGCAATGCCTTCCAGTACGCCAGGCAGGGCGCGACGCGGGATGGTGccatccat                                           <  1:1006366/150‑1 (MQ=255)
                                                                                                      gggTTCGTTGGCATCGAAAAGGATTAACGGGTGCATGTTGCCATATCCGGCATGAAAGACGTTGGCAACACGTAAATCATATTGCTGCGATAAACGGGCAATGCCTTCCAGTACGCCAGGCAGGGCGCGACGCGGGATGGTGccatccat                                           <  1:290724/150‑1 (MQ=255)
                                                                                                      gggTTCGTTGGCATCGAAAAGGATTAACGGGTGCATGTTGCCATATCCGGCATGAAAGACGTTGGCAACACGTAAATCATATTGCTGCGATAAACGGGCAATGCCTTCCAGTACGCCAGGCAGGGCGCGACGCGGGATGGTGccatccat                                           >  2:1499462/1‑150 (MQ=255)
                                                                                                          tCGTTGGCATCGAAAAGGATTAACGGGTGCATGTTGCCATATCCGGCATGAAAGACGTTGGCAACACGTAAATCATATTGCTGCGATAAACGGGCAATGCCTTCCAGTACGCCAGGCAGGGCGCGACGCGGGATGGTGCCATCCATGCag                                       <  1:3321263/150‑1 (MQ=255)
                                                                                                           cGTTGGCATCGAAAAGGATTAACGGGTGCATGTTGCCATATCCGGCATGAAAGACGTTGGCAACACGTAAATCATATTGCTGCGATAAACGGGCAATGCCTTCCAGTACGCCAGGCAGGGCGCGACGCGGGATGGTGCCATCCATGCagt                                      >  1:3177166/1‑150 (MQ=255)
                                                                                                           cGTTGGCATCGAAAAGGATTAACGGGTGCATGTTGCCATATCCGGCATGAAAGACGTTGGCAACACGTAAATCATATTGCTGCGATAAACGGGCAATGCCTTCCAGTACGCCAGGCAGGGCGCGACGCGGGATGGTGCCATCCATGCagt                                      >  1:908856/1‑150 (MQ=255)
                                                                                                            gTTGGCATCGAAAAGGATTAACGGGTGCATGTTGCCATCTCCGGCATGAAAGACGTTGGCAACACGTAAATCATATTGCTGCGATAAACGGGCAATGCCTTCCAGTACGCCAGGCAGGGCGCGACGCGGGATGGTGCCATCCATGCagta                                     >  1:1817475/1‑150 (MQ=255)
                                                                                                            gTTGGCATCGAAAAGGATTAACGGGTGCATGTTGCCATATCCGGCATGAAAGACGTTGGCAACACGTAAATCATATTGCTGCGATAAACGGGCAATGCCTTCCAGTACGCCAGGCAGGGCGCGACGCGGGATGGTGCCATCCATGCagta                                     <  2:1939134/150‑1 (MQ=255)
                                                                                                            gTTGGCATCGAAAAGGATTAACGGGTGCATGTTGCCATATCCGGCATGAAAGACGTTGGCAACACGTAAATCATATTGCTGCGATAAACGGGCAATGCCTTCCAGTACGCCAGGCAGGGCGCGACGCGGGATGGTGCCATCCATGCagta                                     >  2:1816509/1‑150 (MQ=255)
                                                                                                            gTTGGCATCGAAAAGGATTAACGGGTGCATGTTGCCATATCCGGCATGAAAGACGTTGGCAACACGTAAATCATATTGCTGCGATAAACGGGCAATGCCTTCCAGTACGCCAGGCAGGGCGCGACGCGGGATGGTGCCATCCATGCagta                                     >  2:4446605/1‑150 (MQ=255)
                                                                                                             ttGGCATCGAAAAGGATTAACGGGTGCATGTTGCCATATCCGGCATGAAAGACGTTGGCAACACGTAAATCATATTGCTGCGATAAACGGGCAATGCCTTCCAGTACGCCAGGCAGGGCGCGACGCGGGATGGTGCCATCCATGCagtag                                    <  2:2776257/150‑1 (MQ=255)
                                                                                                              tGGCATCGAAAAGGATTAACGGGTGCATGTTGCCATATCCGGCATGAAAGACGTTGGCAACACGTAAATCATATTGCTGCGATAAACGGGCAATGCCTTCCAGTACGCCAGGCAGGGCGCGACGCGGGATGGTGCCATCCATGCagtagt                                   >  2:314970/1‑150 (MQ=255)
                                                                                                              tGGCATCGAAAAGGATTAACGGGTGCATGTTGCCATATCCGGCATGAAAGACGTTGGCAACACGTAAATCATATTGCTGCGATAAACGGGCAATGCCTTCCAGTACGCCAGGCAGGGCGCGACGCGGGATGGTGCCATCCATGCagtagt                                   <  2:4054497/150‑1 (MQ=255)
                                                                                                                 cATCGAAAAGGATTAACGGGTGCATGTTGCCATATCCGGCATGAAAGACGTTGGCAACACGTAAATCATATTGCTGCGATAAACGGGCAATGCCTTCCAGTACGCCAGGCAGGGCGCGACGCGGGATGGTGCCATCCATGCAGTAGTAAt                                <  1:4273136/150‑1 (MQ=255)
                                                                                                                   tCGAAAAGGATTAACGGGTGCATGTTGCCATATCCGGCATGAAAGACGTTGGCAACACGTAAATCATATTGCTGCGATAAACGGGCAATGCCTTCCAGTACGCCAGGCAGGGCGCGACGCGGGATGGTGCCATCCATGCAGTAGTAATcc                              <  1:1611477/150‑1 (MQ=255)
                                                                                                                    cGAAAAGGATTAACGGGTGCATGTTGCCATCTCCGGCATGAAAGACGTTGGCAACACGTAAATCATATTGCTGCGATAAACGGGCAATGCCTTCCAGTACGCCAGGCAGGGCGCGACGCGGGATGGTGCCATCCATGCAGTAGTAATCCg                             <  1:3229427/150‑1 (MQ=255)
                                                                                                                    cGAAAAGGATTAACGGGTGCATGTTGCCATATCCGGCATGAAAGACGTTGGCAACACGTAAATCATATTGCTGCGATAAACGGGCAATGCCTTCCAGTACGCCAGGCAGGGCGCGACGCGGGATGGTGCCATCCATGCAGTAGTAATCCg                             <  1:1620511/150‑1 (MQ=255)
                                                                                                                     gAAAAGGATTAACGGGTGCATGTTGCCATATCCGGCATGAAAGACGTTGGCAACACGTAAATCATATTGCTGCGATAAACGGGCAATGCCTTCCAGTACGCCAGGCAGGGCGCGACGCGGGATGGTGCCATCCATGCAGTAGTAATCCgg                            <  2:3022689/150‑1 (MQ=255)
                                                                                                                      aaaaGGATTAACGGGTGCATGTTGCCATATCCGGCATGAAAGACGTTGGCAACACGTAAATCATATTGCTGCGATAAACGGGCAATGCCTTCCAGTACGCCAGGCAGGGCGCGACGCGGGATGGTGCCATCCATGCAGTAGTAATCCggg                           >  1:2755982/1‑150 (MQ=255)
                                                                                                                      aaaaGGATTAACGGGTGCATGTTGCCATATCCGGCATGAAAGACGTTGGCAACACGTAAATCATATTGCTGCGATAAACGGGCAATGCCTTCCAGTACGCCAGGCAGGGCGCGACGCGGGATGGTGCCATCCATGCAGTAGTAATCCggg                           >  2:2667936/1‑150 (MQ=255)
                                                                                                                        aaGGATTAACGGGTGCATGTTGCCATCTCCGGCATGAAAGACGTTGGCAACACGTAAATCATATTGCTGCGATAAACGGGCAATGCCTTCCAGTACGCCAGGCAGGGCGCGACGCGGGATGGTGCCATCCATGCAGTAGTAATCCGGGga                         <  1:3523901/150‑1 (MQ=255)
                                                                                                                           gATTAACGGGTGCATGTTGCCATATCCGGCATGAAAGACGTTGGCAACACGTAAATCATATTGCTGCGATAAACGGGCAATGCCTTCCAGTACGCCAGGCAGGGCGCGACGCGGGATGGTGCCATCCATGCAGTAGTAATCCGGGGAGAt                      >  2:1108573/1‑150 (MQ=255)
                                                                                                                             ttAACGGGTGCATGTTGCCATCTCCGGCATGAAAGACGTTGGCAACACGTAAATCATATTGCTGCGATAAACGGGCAATGCCTTCCAGTACGCCAGGCAGGGCGCGACGCGGGATGGTGCCATCCATGCAGTAGTAATCCGGGGAGATAc                    <  2:2377222/150‑1 (MQ=255)
                                                                                                                             ttAACGGGTGCATGTTGCCATATCCGGCATGAAAGACGTTGGCAACACGTAAATCATATTGCTGCGATAAACGGGCAATGCCTTCCAGTACGCCAGGCAGGGCGCGACGCGGGATGGTGCCATCCATGCAGTAGTAATCCGGGGAGATAc                    <  2:1131602/150‑1 (MQ=255)
                                                                                                                             ttAACGGGTGCATGTTGCCATATCCGGCATGAAAGACGTTGGCAACACGTAAATCATATTGCTGCGATAAACGGGCAATGCCTTCCAGTACGCCAGGCAGGGCGCGACGCGGGATGGTGCCATCCATGCAGTAGTAATCCGGGGAGATAc                    <  2:3563253/150‑1 (MQ=255)
                                                                                                                              tAACGGGTGCATGTTGCCATATCCGGCATGAAAGACGTTGGCAACACGTAAATCATATTGCTGCGATAAACGGGCAATGCCTTCCAGTACGCCAGGCAGGGCGCGACGCGGGATGGTGCCATCCATGCAGTAGTAATCCGGGGAGATACg                   <  1:4216809/150‑1 (MQ=255)
                                                                                                                              gaaCGGGTGCATGTTGCCATATCCGGCATGAAAGACGTTGGCAACACGTAAATCATATTGCTGCGATAAACGGGCAATGCCTTCCAGTACGCCAGGCAGGGCGCGACGCGGGATGGTGCCATCCATGCAGTAGTAATCCGGGGAGATACg                   <  2:2716113/149‑1 (MQ=255)
                                                                                                                               aaCGGGTGCATGTTGCCATATCCGGCATGAAAGACGTTGGCAACACGTAAATCATATTGCTGCGATAAACGGGCAATGCCTTCCAGTACGCCAGGCAGGGCGCGACGCGGGATGGTGCCATCCATGCAGTAGTAATCCGGGGAGATACGt                  >  2:1411983/1‑150 (MQ=255)
                                                                                                                                     tGCATGTTGCCATATCCGGCATGAAAGACGTTGGCAACACGTAAATCATATTGCTGCGATAAACGGGCAATGCCTTCCAGTACGCCAGGCAGGGCGCGACGCGGGATGGTGCCATCCATGCAGTAGTAATCCGGGGAGATACGTCCTAcc            <  1:2327942/150‑1 (MQ=255)
                                                                                                                                       cATGTTGCCATATCCGGCATGAAAGACGTTGGCAACACGTAAATCATATTGCTGCGATAAACGGGCAATGCCTTCCAGTACGCCAGGCAGGGCGCGACGCGGGATGGTGCCATCCATGCAGTAGTAATCCGGGGAGATACGTCCTAccgc          >  2:1745965/1‑150 (MQ=255)
                                                                                                                                        aTGTTGCCATATCCGGCATGAAAGACGTTGGCAACACGTAAATCATATTGCTGCGATAAACGGGCAATGCCTTCCAGTACGCCAGGCAGGGCGCGACGCGGGATGGTGCCATCCATGCAGTAGTAATCCGGGGAGATACGTCCTAccgcc         >  1:422529/1‑150 (MQ=255)
                                                                                                                                           ttGCCATATCCGGCATGAAAGACGTTGGCAACACGTAAATCATATTGCTGCGATAAACGGGCAATGCCTTCCAGTACGCCAGGCAGGGCGCGACGCGGGATGGTGCCATCCATGCAGTAGTAATCCGGGGAGATACGTCCTACCGCCggg      >  2:883367/1‑150 (MQ=255)
                                                                                                                                             gCCATATCCGGCATGAAAGACGTTGGCAACACGTAAATCATATTGCTGCGATAAACGGGCAATGCCTTCCAGTACGCCAGGCAGGGCGCGACGCGGGATGGTGCCATCCATGCAGTAGTAATCCGGGGAGATACGTCCTACCGCCGGGaa    >  2:1734415/1‑150 (MQ=255)
                                                                                                                                              ccATATCCGGCATGAAAGACGTTGGCAACACGTAAATCATATTGCTGCGATAAACGGGCAATGCCTTCCAGTACGCCAGGCAGGGCGCGACGCGGGATGGTGCCATCCATGCAGTAGTAATCCGGGGAGATACGTCCTACCGCCGGGAAc   >  1:940784/1‑150 (MQ=255)
                                                                                                                                               cATATCCGGCATGAAAGACGTTGGCAACACGTAAATCATATTGCTGCGATAAACGGGCAATGCCTTCCAGTACGCCAGGCAGGGCGCGACGCGGGATGGTGCCATCCATGCAGTAGTAATCCGGGGAGATACGTCCTACCGCCGGGAACg  >  1:2106693/1‑150 (MQ=255)
                                                                                                                                                  |                                                                                                                                                  
TTTTTCTCGCCCGATGCCATGTTCGCCACTGATGCTGCCGCCAACTTCAACGCAGAGTTCGAGGATCTTCCCGCCCAGCTCTTCCGCGCGGGCAAATTCACCGGGTTCGTTGGCATCGAAAAGGATTAACGGGTGCATGTTGCCATCTCCGGCATGAAAGACGTTGGCAACACGTAAATCATATTGCTGCGATAAACGGGCAATGCCTTCCAGTACGCCAGGCAGGGCGCGACGCGGGATGGTGCCATCCATGCAGTAGTAATCCGGGGAGATACGTCCTACCGCCGGGAACG  >  CP009273/3120070‑3120362

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑