Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,174,875 C→A 17.1% R366S (CGT→AGT)  ygiC → ATP‑Grasp family ATPase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,174,8750CA17.1% 774.2 / 112.0 259R366S (CGT→AGT) ygiCATP‑Grasp family ATPase
Reads supporting (aligned to +/- strand):  ref base C (85/129);  new base A (17/28);  total (102/157)
Fisher's exact test for biased strand distribution p-value = 8.68e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 259 total aligned reads displayed.

GAACGGCAAAACCATTGAAGCAGCGGAAGGTCCGTATGGCGAAGAAGGGATGATTGTTCAGCAATTCCACCCGTTACCGAAATTCGGCGACAGCTATATGCTGATTGGTAGCTGGCTGGTGAACGATCAACCCGCCGGAATTGGCATTCGTGAAGACCGTGCATTGATCACCCAGGATATGTCTCGGTTTTATCCACATATTTTTGTTGAATAAGCCACGATACCGGATGGCACTCGCCATCCGGTAATTGTTAGCCTATCTGCACCGACAGCATACTCAGGCTGCCCATTTCTAT  >  CP009273/3174727‑3175022
                                                                                                                                                    |                                                                                                                                                   
gaACGGCAAAACCATTGAAGCAGCGGAAGGTCCGTATGGCGAAGAAGGGATGATTGTTCAGCAATTCCACCCGTTACCGAAATTCGGCGACAGCTATATGCTGATTGGTAGCTGGCTGGTGAACGATCAACCCGCCGGAATTGGCATTCg                                                                                                                                                    <  1:203732/150‑1 (MQ=255)
     gCAAAACCATTGAAGCAGCGGAAGGTCCGTATGGCGAAGAAGGGATGATTGTTCAGCAATTCCACCCGTTACCGAAATTCGGCGACAGCTATATGCTGATTGGTAGCTGGCTGGTGAACGATCAACCCGCCGGAATTGGCATTAGTGAAg                                                                                                                                               <  1:3946689/150‑1 (MQ=255)
     gCAAAACCATTGAAGCAGCGGAAGGTCCGTATGGCGAAGAAGGGATGATTGTTCAGCAATTCCACCCGTTACCGAAATTCGGCGACAGCTATATGCTGATTGGTAGCTGGCTGGTGAACGATCAACCCGCCGGAATTGGCATTAGTGAAg                                                                                                                                               <  1:3705814/150‑1 (MQ=255)
       aaaaCCATTGAAGCAGCGGAAGGTCCGTATGGCGAAGAAGGGATGATTGTTCAGCAATTCCACCCGTTACCGAAATTCGGCGACAGCTATATGCTGATTGGTAGCTGGCTGGTGAACGATCAACCCGCCGGAATTGGCATTCGTGAAGAc                                                                                                                                             >  1:1887524/1‑150 (MQ=255)
         aaCCATTGAAGCAGCGGAAGGTCCGTATGGCGAAGAAGGGATGATTGTTCAGCAATTCCACCCGTTACCGAAATTCGGCGACAGCTATATGCTGATTGGTAGCTGGCTGGTGAACGATCAACCCGCCGGAATTGGCATTAGTGAAGACCg                                                                                                                                           >  1:543793/1‑150 (MQ=255)
         aaCCATTGAAGCAGCGGAAGGTCCGTATGGCGAAGAAGGGATGATTGTTCAGCAATTCCACCCGTTACCGAAATTCGGCGACAGCTATATGCTGATTGGTAGCTGGCTGGTGAACGATCAACCCGCCGGAATTGGCATTAGTGAAGACCg                                                                                                                                           <  2:1450122/150‑1 (MQ=255)
          aCCATTGAAGCAGCGGAAGGTCCGTATGGCGAAGAAGGGATGATTGTTCAGCAATTCCACCCGTTACCGAAATTCGGCGACAGCTATATGCTGATTGGTAGCTGGCTGGTGAACGATCAACCCGCCGGAATTGGCATTAGTGAAGACCGt                                                                                                                                          >  2:2516524/1‑150 (MQ=255)
           ccATTGAAGCAGCGGAAGGTCCGTATGGCGAAGAAGGGATGATTGTTCAGCAATTCCACCCGTTACCGAAATTCGGCGACAGCTATATGCTGATTGGTAGCTGGCTGGTGAACGATCAACCCGCCGGAATTGGCATTCGTGAAGACCGTg                                                                                                                                         <  1:4330132/150‑1 (MQ=255)
           ccATTGAAGCAGCGGAAGGTCCGTATGGCGAAGAAGGGATGATTGTTCAGCAATTCCACCCGTTACCGAAATTCGGCGACAGCTATATGCTGATTGGTAGCTGGCTGGTGAACGATCAACCCGCCGGAATTGGCATTCGTGAAGACCGTg                                                                                                                                         >  1:3955221/1‑150 (MQ=255)
           ccATTGAAGCAGCGGAAGGTCCGTATGGCGAAGAAGGGATGATTGTTCAGCAATTCCACCCGTTACCGAAATTCGGCGACAGCTATATGCTGATTGGTAGCTGGCTGGTGAACGATCAACCCGCCGGAATTGGCATTAGTGAAGACCGTg                                                                                                                                         <  1:3756986/150‑1 (MQ=255)
              ttGAAGCAGCGGAAGGTCCGTATGGCGAAGAAGGGATGATTGTTCAGCAATTCCACCCGTTACCGAAATTCGGCGACAGCTATATGCTGATTGGTAGCTGGCTGGTGAACGATCAACCCGCCGGAATTGGCATTCGTGAAGACCGTGCAt                                                                                                                                      <  2:208769/150‑1 (MQ=255)
                gAAGCAGCGGAAGGTCCGTATGGCGAAGAAGGGATGATTGTTCAGCAATTCCACCCGTTACCGAAATTCGGCGACAGCTATATGCTGATTGGTAGCTGGCTGGTGAACGATCAACCCGCCGGAATTGGCATTCGTGAAGACCGTGCATTg                                                                                                                                    <  2:3883503/150‑1 (MQ=255)
                  agcagcGGAAGGTCCGTATGGCGAAGAAGGGATGATTGTTCAGCAATTCCACCCGTTACCGAAATTCGGCGACAGCTATATGCTGATTGGTAGCTGGCTGGTGAACGATCAACCCGCCGGAATTGGCATTCGTGAAGACCGTGCATTGAt                                                                                                                                  <  2:302668/150‑1 (MQ=255)
                   gcagcGGAAGGTCCGTATGGCGAAGAAGGGATGATTGTTCAGCAATTCCACCCGTTACCGAAATTCGGCGACAGCTATATGCTGATTGGTAGCTGGCTGGTGAACGATCAACCCGCCGGAATTGGCATTAGTGAAGACCGTGCATTGATc                                                                                                                                 <  2:2923596/150‑1 (MQ=255)
                    cagcGGAAGGTCCGTATGGCGAAGAAGGGATGATTGTTCAGCAATTCCACCCGTTACCGAACTTCGGCGACAGCTATATGCTGATTGGTAGCTGGCTGGTGAACGATCAACCCGCCGGAATTGGCATTCGTGAAGACCGTGCATTGATCa                                                                                                                                <  1:3664039/150‑1 (MQ=255)
                    cagcGGAAGGTCCGTATGGCGAAGAAGGGATGATTGTTCAGCAATTCCACCCGTTACCGAAATTCGGCGACAGCTATATGCTGATTGGTAGCTGGCTGGTGAACGATCAACCCGCCGGAATTGGCATTCGTGAAGACCGTGCATTGATCa                                                                                                                                <  1:1132058/150‑1 (MQ=255)
                        ggAAGGTCCGTATGGCGAAGAAGGGATGATTGTTCAGCAATTCCACCCGTTACCGAAATTCGGCGACAGCTATATGCTGATTGGTAGCTGGCTGGTGAACGATCAACCCGCCGGAATTGGCATTCGTGAAGACCGTGCATTGATCACCCa                                                                                                                            >  2:688254/1‑150 (MQ=255)
                        ggAAGGTCCGTATGGCGAAGAAGGGATGATTGTTCAGCAATTCCACCCGTTACCGAAATTCGGCGACAGCTATATGCTGATTGGTAGCTGGCTGGTGAACGATCAACCCGCCGGAATTGGCATTCGTGAAGACCGTGCATTGATCACCCa                                                                                                                            >  2:3105180/1‑150 (MQ=255)
                        ggAAGGTCCGTATGGCGAAGAAGGGATGATTGTTCAGCAATTCCACCCGTTACCGAAATTCGGCGACAGCTATATGCTGATTGGTAGCTGGCTGGTGAACGATCAACCCGCCGGAATTGGCATTAGTGAAGACCGTGCATTGATCACCCa                                                                                                                            >  1:3289181/1‑150 (MQ=255)
                        ggAAGGTCCGTATGGCGAAGAAGGGATGATTGTTCAGCAATTCCACCCGTTACCGAAATTCGGCGACAGCTATATGCTGATTGGTAGCTGGCTGGTGAACGATCAACCCGCCGGAATTGGCATTAGTGAAGACCGTGCATTGATCACCCa                                                                                                                            >  1:3289305/1‑150 (MQ=255)
                            ggTCCGTATGGCGAAGAAGGGATGATTGTTCAGCAATTCCACCCGTTACCGAAATTCGGCGACAGCTATATGCTGATTGGTAGCTGGCTGGTGAACGATCAACCCGCCGGAATTGGCATTCGTGAAGACCGTGCATTGATCACCCAGGat                                                                                                                        <  1:2985432/150‑1 (MQ=255)
                            ggTCCGTATGGCGAAGAAGGGATGATTGTTCAGCAATTCCACCCGTTACCGAAATTCGGCGACAGCTATATGCTGATTGGTAGCTGGCTGGTGAACGATCAACCCGCCGGAATTGGCATTCGTGAAGACCGTGCATTGATCACCCAGGat                                                                                                                        >  1:3768151/1‑150 (MQ=255)
                             gTCCGTATGGCGAAGAAGGGATGATTGTTCAGCAATTCCACCCGTTACCGAAATTCGGCGACAGCTATATGCTGATTGGTAGCTGGCTGGTGAACGATCAACCCGCCGGAATGGGCATTCGTGAAGACCGTGCATTGATCACCCAGGata                                                                                                                       <  1:2153021/150‑1 (MQ=255)
                                 gTATGGCGAAGAAGGGATGATTGTTCAGCAATTCCACCCGTTACCGAAATTCGGCGACAGCTATATGCTGATTGGTAGCTGGCTGGTGAACGATCAACCCGCCGGAATTGGCATTCGTGAAGACCGTGCATTGATCACCCAGGATATGtc                                                                                                                   >  1:4445478/1‑150 (MQ=255)
                                  tATGGCGAAGAAGGGATGATTGTTCAGCAATTCCACCCGTTACCGAAATTCGGCGACAGCTATATGCTGATTGGTAGCTGGCTGGTGAACGATCAACCCGCCGGAATTGGCATTCGTGAAGACCGTGCATTGATCACCCAGGATATGtct                                                                                                                  >  2:2298946/1‑150 (MQ=255)
                                   aTGGCGAAGAAGGGATGATTGTTCAGCAATTCCACCCGTTACCGAAATTCGGCGACAGCTATATGCTGATTGGTAGCTGGCTGGTGAACGATCAACCCGCCGGAATTGGCATTCGTGAAGACCGTGCATTGATCACCCAGGATATGtctc                                                                                                                 >  2:93494/1‑150 (MQ=255)
                                   aTGGCGAAGAAGGGATGATTGTTCAGCAATTCCACCCGTTACCGAAATTCGGCGACAGCTATATGCTGATTGGTAGCTGGCTGGTGAACGATCAACCCGCCGGAATTGGCATTCGTGAAGACCGTGCATTGATCACCCAGGATATGtctc                                                                                                                 >  1:1399149/1‑150 (MQ=255)
                                    tGGCGAAGAAGGGATGATTGTTCAGCAATTCCACCCGTTACCGAAATTCGGCGACAGCTATATGCTGATTGGTAGCTGGCTGGTGAACGATCAACCCGCCGGAATTGGCATTCGTGAAGACCGTGCATTGATCACCCAGGATATGTCTCg                                                                                                                <  2:2290751/150‑1 (MQ=255)
                                        gaagaaGGGATGATTGTTCAGCAATTCCACCCGTTACCGAAATTCGGCGACAGCTATATGCTGATTGGTAGCTGGCTGGTGAACGATCAACCCGCCGGAATTGGCATTCGTGAAGACCGTGCATTGATCACCCAGGATATGTCTCGGttt                                                                                                            <  2:4194620/150‑1 (MQ=255)
                                            aagGGATGATTGTTCAGCAATTCCACCCGTTACCGAAATTCGGCGACAGCTATATGCTGATTGGTAGCTGGCTGGTGAACGATCAACCCGCCGGAATTGGCATTCGTGAAGACCGTGCATTGATCACCCAGGATATGTCTCGGTTTTATc                                                                                                        <  1:720849/150‑1 (MQ=255)
                                            aagGGATGATTGTTCAGCAATTCCACCCGTTACCGAAATTCGGCGACAGCTATATGCTGATTGGTAGCTGGCTGGTGAACGATCAACCCGCCGGAATTGGCATTCGTGAAGACCGTGCATTGATCACCCAGGATATGTCTCGGTTTTATc                                                                                                        <  1:393208/150‑1 (MQ=255)
                                             agGGATGATTGTTCAGCAATTCCACCCGTTACCGAAATTCGGCGACAGCTATATGCTGATTGGTAGCTGGCTGGTGAACGATCAACCCGCCGGAATTGGCATTCGTGAAGACCGTGCATTGATCACCCAGGATATGTCTCGGTTTTATcc                                                                                                       >  1:441090/1‑150 (MQ=255)
                                                gatgatTGTTCAGCAATTCCACCCGTTACCGAAATTCGGCGACAGCTATATGCTGATTGGTAGCTGGCTGGTGAACGATCAACCCGCCGGAATTGGCATTCGTGAAGACCGTGCATTGATCACCCAGGATATGTCTCGGTTTTATCcaca                                                                                                    <  2:3520617/150‑1 (MQ=255)
                                                gatgatTGTTCAGCAATTCCACCCGTTACCGAAATTCGGCGACAGCTATATGCTGATTGGTAGCTGGCTGGTGAACGATCAACCCGCCGGAATTGGCATTCGTGAAGACCGTGCATTGATCACCCAGGATATGTCTCGGTTTTATCcaca                                                                                                    <  1:1967814/150‑1 (MQ=255)
                                                  tgatTGTTCAGCAATTCCACCCGTTACCGAAATTCGGCGACAGCTATATGCTGATTGGTAGCTGGCTGGTGAACGATCAACCCGCCGGAATTGGCATTCGTGAAGACCGTGCATTGATCACCCAGGATATGTCTCGGTTTTATCCACata                                                                                                  >  1:3818627/1‑150 (MQ=255)
                                                     ttGTTCAGCAATTCCACCCGTTACCGAAATTCGGCGACAGCTATATGCTGATTGGTAGCTGGCTGGTGAACGATCAACCCGCCGGAATTGGCATTCGTGAAGACCGTGCATTGATCACCCAGGATATGTCTCGGTTTTATCCACATAttt                                                                                               <  1:41380/150‑1 (MQ=255)
                                                       gTTCAGCAATTCCACCCGTTACCGAAATTCGGCGACAGCTATATGCTGATTGGTAGCTGGCTGGTGAACGATCAACCCGCCGGAATTGGCATTCGTGAAGACCGTGCATTGATCACCCAGGATATGTCTCGGTTTTATCCACATAttttt                                                                                             <  2:2166681/150‑1 (MQ=255)
                                                       gTTCAGCAATTCCACCCGTTACCGAAATTCGGCGACAGCTATATGCTGATTGGTAGCTGGCTGGTGAACGATCAACCCGCCGGAATTGGCATTAGTGAAGACCGTGCATTGATCACCCAGGATATGTCTCGGTTTTATCCACATAttttt                                                                                             <  1:4354511/150‑1 (MQ=255)
                                                        ttCAGCAATTCCACCCGTTACCGAAATTCGGCGACAGCTATATGCTGATTGGTAGCTGGCTGGTGAACGATCAACCCGCCGGAATTGGCATTAGTGAAGACCGTGCATTGATCACCCAGGATATGTCTCGGTTTTATCCACATATTTttg                                                                                            >  2:1876340/1‑150 (MQ=255)
                                                          cAGCAATTCCACCCGTTACCGAAATTCGGCGACAGCTATATGCTGATTGGTAGCTGGCTGGTGAACGATCAACCCGCCGGAATTGGCATTCGTGAAGACCGTGCATTGATCACCCAGGATATGTCTCGGTTTTATCCACATATTTttgtt                                                                                          >  1:1440268/1‑150 (MQ=255)
                                                              aaTTCCACCCGTTACCGAAATTCGGCGACAGCTATATGCTGATTGGTAGCTGGCTGGTGAACGATCAACCCGCCGGAATTGGCATTCGTGAAGACCGTGCATTGATCACCCAGGATATGTCTCGGTTTTATCCACATATTTTTGTTGAAt                                                                                      <  1:809078/150‑1 (MQ=255)
                                                              aaTTCCACCCGTTACCGAAATTCGGCGACAGCTATATGCTGATTGGTAGCTGGCTGGTGAACGATCAACCCGCCGGAATTGGCATTCGTGAAGACCGTGCATTGATCACCCAGGATATGTCTCGGTTTTATCCACATATTTTTGTTGAAt                                                                                      <  1:3071246/150‑1 (MQ=255)
                                                               aTTCCACCCGTTACCGAAATTCGGCGACAGCTATATGCTGATTGGTAGCTGGCTGGTGAACGATCAACCCGCCGGAATTGGCATTCGTGAAGACCGTGCATTGATCACCCAGGATATGTCTCGGTTTTATCCACATATTTTTGTTGAATa                                                                                     <  2:1208227/150‑1 (MQ=255)
                                                               aTTCCACCCGTTACCGAAATTCGGCGACAGCTATATGCTGATTGGTAGCTGGCTGGTGAACGATCAACCCGCCGGAATTGGCATTCGTGAAGACCGTGCATTGATCACCCAGGATATGTCTCGGTTTTATCCACATATTTTTGTTGAATa                                                                                     <  1:1250129/150‑1 (MQ=255)
                                                                ttCCACCCGTTACCGAAATTCGGCGACAGCTATATGCTGATTGGTAGCTGGCTGGTGAACGATCAACCCGCCGGAATTGGCATTCGTGAAGACCGTGCATTGATCACCCAGGATATGTCTCGGTTTTATCCACATATTTTTGTTGAATaa                                                                                    <  2:332492/150‑1 (MQ=255)
                                                                 tCCACCCGTTACCGAAATTCGGCGACAGCTATATGCTGATTGGTAGCTGGCTGGTGAACGATCAACCCGCCGGAATTGGCATTCGTGAAGACCGTGCATTGATCACCCAGGATATGTCTCGGTTTTATCCACATATTTTTGTTGAATAAg                                                                                   >  1:2169338/1‑150 (MQ=255)
                                                                 tCCACCCGTTACCGAAATTCGGCGACAGCTATATGCTGATTGGTAGCTGGCTGGTGAACGATCAACCCGCCGGAATTGGCATTCGTGAAGACCGTGCATTGATCACCCAGGATATGTCTCGGTTTTATCCACATATTTTTGTTGAATAAg                                                                                   <  2:1310251/150‑1 (MQ=255)
                                                                 tCCACCCGTTACCGAAATTCGGCGACAGCTATATGCTGAGTGGTAGCTGGCTGGTGAACGATCAACCCGCCGGAATTGGCATTCGTGAAGACCGTGCATTGATCACCCAGGATATGTCTCGGTTTTATCCACATATTTTTGTTGAATAAg                                                                                   >  2:4416388/1‑150 (MQ=255)
                                                                     cccGTTACCGAAATTCGGCGACAGCTATATGCTGATTGGTAGCTGGCTGGTGAACGATCAACCCGCCGGAATTGGCATTCGTGAAGACCGTGCATTGATCACCCAGGATATGTCTCGGTTTTATCAACATATTTTTGTTGAATAAGCCAc                                                                               >  2:3997530/1‑150 (MQ=255)
                                                                        gTTACCGAAATTCGGCGACAGCTATATGCTGATTGGTAGCTGGCTGGTGAACGATCAACCCGCCGGAATTGGCATTCGTGAAGACCGTGCATTGATCACCCAGGATATGTCTCGGTTTTATCCACATATTTTTGTTGAATAAGCCACGAt                                                                            >  1:3333435/1‑150 (MQ=255)
                                                                         ttACCGAAATTCGGCGACAGCTATATGCTGATTGGTAGCTGGCTGGTGAACGATCAACCCGCCGGAATTGGCATTCGTGAAGACCGTGCATTGATCACCCAGGATATGTCTCGGTTTTATCCACATATTTTTGTTGAATAAGCCACGATa                                                                           >  1:2957077/1‑150 (MQ=255)
                                                                           aCCGAAATTCGGCGACAGCTATATGCTGATTGGTAGCTGGCTGGTGAACGATCAACCCGCCGGAATTGGCATTCGTGAAGACCGTGCATTGATCACCCAGGATATGTCTCGGTTTTATCCACATATTTTTGTTGAATAAGCCACGATAcc                                                                         <  1:2742589/150‑1 (MQ=255)
                                                                            ccGAAATTCGGCGACAGCTATATGCTGATTGGTAGCTGGCTGGTGAACGATCAACCCGCCGGAATTGGCATTCGTGAAGACCGTGCATTGATCACCCAGGATATGTCTCGGTTTTATCCACATATTTTTGTTGAATAAGCCACGATACCg                                                                        <  2:2212497/150‑1 (MQ=255)
                                                                               aaaTTCGGCGACAGCTATATGCTGATTGGTAGCTGGCTGGTGAACGATCAACCCGCCGGAATTGGCATTCGTGAAGACCGTGCATTGATCACCCAGGATATGTCTCGGTTTTATCCACATATTTTTGTTGAATAAGCCACGATACCGGAt                                                                     >  2:4043794/1‑150 (MQ=255)
                                                                               aaaTTCGGCGACAGCTATATGCTGATTGGTAGCTGGCTGGTGAACGATCAACCCGCCGGAATTGGCATTCGTGAAGACCGTGCATTGATCACCCAGGATATGTCTCGGTTTTATCCACATATTTTTGTTGAATAAGCCACGATACCGGAt                                                                     <  1:1287977/150‑1 (MQ=255)
                                                                               aaaTTCGGCGACAGCTATATGCTGATTGGTAGCTGGCTGGTGAACGATCAACCCGCCGGAATTGGCATTCGTGAAGACCGTGCATTGATCACCCAGGATATGTCTCGGTTTTATCCACATATTTTTGTTGAATAAGCCACGATACCGGAt                                                                     <  1:1287948/150‑1 (MQ=255)
                                                                                aaTTCGGCGACAGCTATATGCTGATTGGTAGCTGGCTGGTGAACGATCAACCCGCCGGAATTGGCATTCGTGAAGACCGTGCATTGATCACCCAGGATATGTCTCGGTTTTATCCACATATTTTTGTTGAATAAGCCACGATACCGGATg                                                                    <  2:3837404/150‑1 (MQ=255)
                                                                                  ttCGGCGACAGCTATATGCTGATTGGTAGCTGGCTGGTGAACGATCAACCCGCCGGAATTGGCATTCGTGAAGACCGTGCATTGATCACCCAGGATATGTCTCGGTTTTATCCACATATTTTTGTTGAATAAGCCACGATACCGGATGGc                                                                  <  2:3711044/150‑1 (MQ=255)
                                                                                   tCGGCGACAGCTATATGCTGATTGGTAGCTGGCTGGTGAACGATCAACCCGCCGGAATTGGCATTCGTGAAGACCGTGCATTGATCACCCAGGATATGTCTCGGTTTTATCCACATATTTTTGTTGAATAAGCCACGATACCGGATGGCa                                                                 <  1:3136089/150‑1 (MQ=255)
                                                                                   tCGGCGACAGCTATATGCTGATTGGTAGCTGGCTGGTGAACGATCAACCCGCCGGAATTGGCATTCGTGAAGACCGTGCATTGATCACCCAGGATATGTCTCGGTTTTATCCACATATTTTTGTTGAATAAGCCACGATACCGGATGGCa                                                                 <  1:3791579/150‑1 (MQ=255)
                                                                                      gCGACAGCTATATGCTGATTGGTAGCTGGCTGGTGAACGATCAACCCGCCGGAATTGGCATTCGTGAAGACCGTGCATTGATCACCCAGGATATGTCTCGGTTTTATCCACATATTTTTGTTGAATAAGCCACGATACCGGATGGCACTc                                                              <  1:69658/150‑1 (MQ=255)
                                                                                       cGACAGCTATATGCTGATTGGTAGCTGGCTGGTGAACGATCAACCCGCCGGAATTGGCATTCGTGAAGACCGTGCATTGATCACCCAGGATATGTCTCGGTTTTATCCACATATTTTTGTTGAATAAGCCACGATACCGGATGGCACTCg                                                             <  1:406277/150‑1 (MQ=255)
                                                                                             cTATATGCTGATTGGTAGCTGGCTGGTGAACGATCAACCCGCCGGAATTGGCATTCGTGAAGACCGTGCATTGATCACCCAGGATATGTCTCGGTTTTATCCACATATTTTTGTTGAATAAGCCACGATACCGGATGGCACTCGCCATcc                                                       <  2:43122/150‑1 (MQ=255)
                                                                                              tataTGCTGATTGGTAGCTGGCTGGTGAACGATCAACCCGCCGGAATTGGCATTCGTGAAGACCGTGCATTGATCACCCAGGATATGTCTCGGTTTTATCCACATATTTTTGTTGAATAAGCCACGATACCGGATGGCACTCGCCATCCg                                                      <  1:2829255/150‑1 (MQ=255)
                                                                                              tataTGCTGATTGGTAGCTGGCTGGTGAACGATCAACCCGCCGGAATTGGCATTCGTGAAGACCGTGCATTGATCACCCAGGATATGTCTCGGTTTTATCCACATATTTTTGTTGAATAAGCCACGATACCGGATGGCACTCGCCATCCg                                                      >  2:2670409/1‑150 (MQ=255)
                                                                                               atatGCTGATTGGTAGCTGGCTGGTGAACGATCAACCCGCCGGAATTGGCATTCGTGAAGACCGTGCATTGATCACCCAGGATATGTCTCGGTTTTATCCACATATTTTTGTTGAATAAGCCACGATACCGGATGGCACTCGCCATCCgg                                                     >  1:3522307/1‑150 (MQ=255)
                                                                                                tatGCTGATTGGTAGCTGGCTGGTGAACGATCAACCCGCCGGAATTGGCATTCGTGAAGACCGTGCATTGATCACCCAGGATATGTCTCGGTTTTATCCACATATTTTTGTTGAATAAGCCACGATACCGGATGGCACTCGCCATCCGGt                                                    <  1:1878910/150‑1 (MQ=255)
                                                                                                 atGCTGATTGGTAGCTGGCTGGTGAACGATCAACCCGCCGGAATTGGCATTCGTGAAGACCGTGCATTGATCACCCAGGATATGTCTCGGTTTTATCCACATATTTTTGTTGAATAAGCCACGATACCGGATGGCACTCGCCATCCGGTa                                                   >  1:932220/1‑150 (MQ=255)
                                                                                                 atGCTGATTGGTAGCTGGCTGGTGAACGATCAACCCGCCGGAATTGGCATTCGTGAAGACCGTGCATTGATCACCCAGGATATGTCTCGGTTTTATCCACATATTTTTGTTGAATAAGCCACGATACCGGATGGCACTCGCCATCCGGTa                                                   >  1:2569524/1‑150 (MQ=255)
                                                                                                  tGCTGATTGGTAGCTGGCTGGTGAACGATCAACCCGCCGGAATTGGCATTCGTGAAGACCGTGCATTGATCACCCAGGATATGTCTCGGTTTTATCCACATATTTTTGTTGAATAAGCCACGATACCGGATGGCACTCGCCATCCGGTaa                                                  <  1:3576355/150‑1 (MQ=255)
                                                                                                  tGCTGATTGGTAGCTGGCTGGTGAACGATCAACCCGCCGGAATTGGCATTCGTGAAGACCGTGCATTGATCACCCAGGATATGTCTCGGTTTTATCCACATATTTTTGTTGAATAAGCCACGATACCGGATGGCACTCGCCATCCGGTaa                                                  <  2:84278/150‑1 (MQ=255)
                                                                                                  ggCTGATTGGTAGCTGGCTGGTGAACGATCAACCCGCCGGAATTGGCATTCGTGAAGACCGTGCATTGATCACCCAGGATATGTCTCGGTTTTATCCACATATTTTTGTTGAATAAGCCACGATACCGGATGGCACTCGCCATCCGGTaa                                                  <  2:650614/149‑1 (MQ=255)
                                                                                                   gCTGATTGGTAGCTGGCTGGTGAACGATCAACCCGCCGGAATTGGCATTCGTGAAGACCGTGCATTGATCACCCAGGATATGTCTCGGTTTTATCCACATATTTTTGTTGAATAAGCCACGATACCGGATGGCACTCGCCATCCGGTAAt                                                 <  2:1451663/150‑1 (MQ=255)
                                                                                                    cTGATTGGTAGCTGGCTGGTGAACGATCAACCCGCCGGAATTGGCATTCGTGAAGACCGTGCATTGATCACCCAGGATATGTCTCGGTTTTATCCACATATTTTTGTTGAATAAGCCACGATACCGGATGGCACTCGCCATCCGGTAAtt                                                <  1:2453559/150‑1 (MQ=255)
                                                                                                     tGATTGGTAGCTGGCTGGTGAACGATCAACCCGCCGGAATTGGCATTCGTGAAGACCGTGCATTGATCACCCAGGATATGTCTCGGTTTTATCCACATATTTTTGTTGAATAAGCCACGATACCGGATGGCACTCGCCATCCGGTAATTg                                               <  2:1604516/150‑1 (MQ=255)
                                                                                                       aTTGGTAGCTGGCTGGTGAACGATCAACCCGCCGGAATTGGCATTCGTGAAGACCGTGCATTGATCACCCAGGATATGTCTCGGTTTTATCCACATATTTTTGTTGAATAAGCCACGATACCGGATGGCACTCGCCATCCGGTAATTGtt                                             >  2:3819805/1‑150 (MQ=255)
                                                                                                         tGGTAGCTGGCTGGTGAACGATCAACCCGCCGGAATTGGCATTCGTGAAGACCGTGCATTGATCACCCAGGATATGTCTCGGTTTTATCCACATATTTTTGTTGAATAAGCCACGATACCGGATGGCACTCGCCATCCGGTAATTGTTAg                                           <  2:1270093/150‑1 (MQ=255)
                                                                                                           gTAGCTGGCTGGTGAACGATCAACCCGCCGGAATTGGCATTCGTGAAGACCGTGCATTGATCACCCAGGATATGTCTCGGTTTTATCCACATATTTTTGTTGAATAAGCCACGATACCGGATGGCACTCGCCATCCGGTAATTGTTAGcc                                         <  1:295214/150‑1 (MQ=255)
                                                                                                            tAGCTGGCTGGTGAACGATCAACCCGCCGGAATTGGCATTCGTGAAGACCGTGCATTGATCACCCAGGATATGTCTCGGTTTTATCCACATATTTTTGTTGAATAAGCCACGATACCGGATGGCACTCGCCATCCGGTAATTGTTAGCCt                                        >  1:4072900/1‑150 (MQ=255)
                                                                                                             aGCTGGCTGGTGAACGATCAACCCGCCGGAATTGGCATTCGTGAAGACCGTGCATTGATCACCCAGGATATGTCTCGGTTTTATCCACATATTTTTGTTGAATAAGCCACGATACCGGATGGCACTCGCCATCCGGTAATTGTTAGCCTa                                       <  1:46267/150‑1 (MQ=255)
                                                                                                               ctggctggTGAACGATCAACCCGCCGGAATTGGCATTCGTGAAGACCGTGCATTGATCACCCAGGATATGTCTCGGTTTTATCCACATATTTTTGTTGAATAAGCCACGATACCGGATGGCACTCGCCATCCGGTAATTGTTAGCCTATc                                     <  2:3602451/150‑1 (MQ=255)
                                                                                                                tggctggTGAACGATCAACCCGCCGGAATTGGCATTCGTGAAGACCGTGCATTGATCACCCAGGATATGTCTCGGTTTTATCCACATATTTTTGTTGAATAAGCCACGATACCGGATGGCACTCGCCATCCGGTAATTGTTAGCCTATCt                                    >  2:4096056/1‑150 (MQ=255)
                                                                                                                 ggctggTGAACGATCAACCCGCCGGAATTGGCATTAGTGAAGACCGTGCATTGATCACCCAGGATATGTCTCGGTTTTATCCACATATTTTTGTTGAATAAGCCACGATACCGGATGGCACTCGCCATCCGGTAATTGTTAGCCTATCTg                                   <  2:2235080/150‑1 (MQ=255)
                                                                                                                   ctggTGAACGATCAACCCGCCGGAATTGGCATGAGTGAAGACCGTGCATTGATCACCCAGGATATGTCTCGGTTTTATCCACATATTTTTGTTGAATAAGCCACGATACCGGATGGCACTCGCCATCCGGTAATTGTTAGCCTATCTGCa                                 <  2:3935172/150‑1 (MQ=255)
                                                                                                                     ggTGAACGATCAACCCGCCGGAATTGGCATTCGTGAAGACCGTGCATTGATCACCCAGGATATGTCTCGGTTTTATCCACATATTTTTGTTGAATAAGCCACGATACCGGATGGCACTCGCCATCCGGTAATTGTTAGCCTATCTGCAcc                               <  2:2130862/150‑1 (MQ=255)
                                                                                                                      gTGAACGATCAACCCGCCGGAATTGGCATTAGTGAAGACCGTGCATTGATCACCCAGGATATGTCTCGGTTTTATCCACATATTTTTGTTGAATAAGCCACGATACCGGATGGCACTCGCCATCCGGTAATTGTTAGCCTATCTGCACCg                              >  1:2656551/1‑150 (MQ=255)
                                                                                                                        gAACGATCAACCCGCCGGAATTGGCATTCGTGAAGACCGTGCATTGATCACCCAGGATATGTCTCGGTTTTATCCACATATTTTTGTTGAATAAGCCACGATACCGGATGGCACTCGCCATCCGGTAATTGTTAGCCTATCTGCACCGAc                            >  2:924479/1‑150 (MQ=255)
                                                                                                                             aTCAACCCGCCGGAATTGGCATGAGTGAAGACCGTGCATTGATCACCCAGGATATGTCTCGGTTTTATCCACATATTTTTGTTGAATAAGCCACGATACCGGATGGCACTCGCCATCCGGTAATTGTTAGCCTATCTGCACCGACAGCAt                       <  2:3274377/150‑1 (MQ=255)
                                                                                                                              tCAACCCGCCGGAATTGGCATTCGTGAAGACCGTGCATTGATCACCCAGGATATGTCTCGGTTTTATCCACATATTTTTGTTGAATAAGCCACGATACCGGATGGCACTCGCCATCCGGTAATTGTTAGCCTATCTGCACCGACAGCATa                      <  1:1730544/150‑1 (MQ=255)
                                                                                                                                  cccGCCGGAATTGGCATTCGTGAAGACCGTGCATTGATCACCCAGGATATGTCTCGGTTTTATCCACATATTTTTGTTGAATAAGCCACGATACCGGATGGCACTCGCCATCCGGTAATTGTTAGCCTATCTGCACCGACAGCATACTCa                  <  1:1563517/150‑1 (MQ=255)
                                                                                                                                         gAATTGGCATTCGTGAAGACCGTGCATTGATCACCCAGGATATGTCTCGGTTTTATCCACATATTTTTGTTGAATAAGCCACGATACCGGATGGCACTCGCCATCCGGTAATTGTTAGCCTATCTGCACCGACAGCATACTCAGGCTGcc           >  1:1861947/1‑150 (MQ=255)
                                                                                                                                         gAATTGGCATTAGTGAAGACCGTGCATTGATCACCCAGGATATGTCTCGGTTTTATCCACATATTTTTGTTGAATAAGCCACGATACCGGATGGCACTCGCCATCCGGTAATTGTTAGCCTATCTGCACCGACAGCATACTCAGGCTGcc           <  1:642508/150‑1 (MQ=255)
                                                                                                                                          aaTTGGCATTCGTGAAGACCGTGCATTGATCACCCAGGATATGTCTCGGTTTTATCCACATATTTTTGTTGAATAAGCCACGATACCGGATGGCACTCGCCATCCGGTAATTGTTAGCCTATCTGCACCGACAGCATACTCAGGCTGccc          <  2:1670413/150‑1 (MQ=255)
                                                                                                                                          aaTTGGCATTCGTGAAGACCGTGCATTGATCACCCAGGATATGTCTCGGTTTTATCCACATATTTTTGTTGAATAAGCCACGATACCGGATGGCACTCGCCATCCGGTAATTGTTAGCCTATCTGCACCGACAGCATACTCAGGCTGccc          <  2:596904/150‑1 (MQ=255)
                                                                                                                                          aaTTGGCATTCGTGAAGACCGTGCATTGATCACCCAGGATATGTCTCGGTTTTATCCACATATTTTTGTTGAATAAGCCACGATACCGGATGGCACTCGCCATCCGGTAATTGTTAGCCTATCTGCACCGACAGCATACTCAGGCTGacc          <  2:247637/150‑1 (MQ=255)
                                                                                                                                            ttGGCATTCGTGAAGACCGTGCATTGATCACCCAGGATATGTCTCGGTTTTATCCACATATTTTTGTTGAATAAGCCACGATACCGGATGGCACTCGCCATCCGGTAATTGTTAGCCTATCTGCACCGACAGCATACTCAGGCTGCCCAt        <  2:759667/150‑1 (MQ=255)
                                                                                                                                            atGGCATTCGTGAAGACCGTGCATTGATCACCCCGGATATGTCTCGGTTTTATCCACATATTTTTGTTGAATAAGCCACGATACCGGATGGCACTCGCCATCCGGTAATTGTTAGCCTATCTGCACCGACAGCATACTCAGGCTGCCCAt        <  2:759539/149‑1 (MQ=255)
                                                                                                                                                  ttCGTGAAGACCGTGCATTGATCACCCAGGATATGTCTCGGTTTTATCCACATATTTTTGTTGAATAAGCCACGATACCGGATGGCACTCGCCATCCGGTAATTGTTAGCCTATCTGCACCGACAGCATACTCAGGCTGCCCATTTCtat  <  1:3543693/150‑1 (MQ=255)
                                                                                                                                                  ttCGTGAAGACCGTGCATTGATCACCCAGGATATGTCTCGGTTTTATCCACATATTTTTGTTGAATAAGCCACGATACCGGATGGCACTCGCCATCCGGTAATTGTTAGCCTATCTGCACCGACAGCATACTCAGGCTGCCCATTTCtat  <  1:165298/150‑1 (MQ=255)
                                                                                                                                                  ttCGTGAAGACCGTGCATTGATCACCCAGGATATGTCTCGGTTTTATCCACATATTTTTGTTGAATAAGCCACGATACCGGATGGCACTCGCCATCCGGTAATTGTTAGCCTATCTGCACCGACAGCATACTCAGGCTGCCCATTTCtat  <  2:3672312/150‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                   
GAACGGCAAAACCATTGAAGCAGCGGAAGGTCCGTATGGCGAAGAAGGGATGATTGTTCAGCAATTCCACCCGTTACCGAAATTCGGCGACAGCTATATGCTGATTGGTAGCTGGCTGGTGAACGATCAACCCGCCGGAATTGGCATTCGTGAAGACCGTGCATTGATCACCCAGGATATGTCTCGGTTTTATCCACATATTTTTGTTGAATAAGCCACGATACCGGATGGCACTCGCCATCCGGTAATTGTTAGCCTATCTGCACCGACAGCATACTCAGGCTGCCCATTTCTAT  >  CP009273/3174727‑3175022

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑