Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,193,086 A→T 13.7% I413I (ATT→ATA)  ygiF ← inorganic triphosphatase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,193,0860AT13.7% 844.3 / 112.1 277I413I (ATT→ATA) ygiFinorganic triphosphatase
Reads supporting (aligned to +/- strand):  ref base A (144/95);  new base T (25/13);  total (169/108)
Fisher's exact test for biased strand distribution p-value = 5.93e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.90e-01

Only 100 of 283 total aligned reads displayed.

GCTCTGGCAGCCGCTCAACAACGGTCTGCCAGTACTGCTGTAACGGTGAAGAGAGCGGCTTCATAAGCGATTTTATCCTTGCCTGATTAACGTTTTCCGCTGTGCAACCAGAACGGTTCCTGATTGTTTGCCTCATTACGGAAATGTTCAATTTCGATGCGTTGCCCGGTCGCAATAGCGTGATGCAGCCCCTGCCAGTTCTCCAGCCAGGCTTGCGCGACGACAGGATCATAGTAACCCGCCAGCAACAGTATTGAGTCAATATCACGCGTCAGGCGTGGCAACTGGTCACGGTAGCG  >  CP009273/3192937‑3193235
                                                                                                                                                     |                                                                                                                                                     
   ctGGCAGCCGCTCAACAACGGTCTGCCAGTACTGCTGTAACGGTGAAGAGAGCGGCTTCATAAGCGATTTTATCCTTGCCTGATTAACGTTTTCCGCTGTGCAACCAGAACGGTTCCTGATTGTTTGCCTCATTACGGAAATGTTCTAtt                                                                                                                                                    >  1:1471212/1‑150 (MQ=255)
    tGGCAGCCGCTCAACAACGGTCTGCCAGTACTGCTGTAACGGTGAAGAGAGCGGCTTCATAAGCGATTTTATCCTTGCCTGATTAACGTTTTCCGCTGTGCAACCAGAACGGTTCCTGATTGTTTGCCTCATTACGGAAATGTTCAAttt                                                                                                                                                   >  2:3289157/1‑150 (MQ=255)
      gCAGCCGCTCAACAACGGTCTGCCAGTACTGCTGTAACGGTGAAGAGAGCGGCTTCATAAGCGATTTTATCCTTGCCTGATTAACGTTTTCCGCTGTGCAACCAGAACGGTTCCTGATTGTTTGCCTCATTACGGAAATGTTCTATTTCg                                                                                                                                                 <  1:652002/150‑1 (MQ=255)
       cAGCCGCTCAACAACGGTCTGCCAGTACTGCTGTAACGGTGAAGAGAGCGGCTTCATAAGCGATTTTATCCTTGCCTGATTAACGTTTTCCGCTGTGCAACCAGAACGGTTCCTGATTGTTTGCCTCATTACGGAAATGTTCAATTTCGa                                                                                                                                                <  1:1389768/150‑1 (MQ=255)
        aGCCGCTCAACAACGGTCTGCCAGTACTGCTGTAACGGTGAAGAGAGCGGCTTCATAAGCGATTTTATCCTTGCCTGATTAACGTTTTCCGCTGTGCAACCAGAACGGTTCCTGATTGTTTGCCTCATTACGGAAATGTTCAATTTCGAt                                                                                                                                               >  1:861333/1‑150 (MQ=255)
        aGCCGCTCAACAACGGTCTGCCAGTACTGCTGTAACGGTGAAGAGAGCGGCTTCATAAGCGATTTTATCCTTGCCTGATTAACGTTTTCCGCTGTGCAACCAGAACGGTTCCTGATTGTTTGCCTCATTACGGAAATGTTCAATTTCGAt                                                                                                                                               >  2:2150371/1‑150 (MQ=255)
           cGCTCAACAACGGTCTGCCAGTACTGCTGTAACGGTGAAGAGAGCGGCTTCATAAGCGATTTTATCCTTGCCTGATTAACGTTTTCCGCTGTGCAACCAGAACGGTTCCTGATTGTTTGCCTCATTACGGAAATGTTCTATTTCGATGCg                                                                                                                                            >  2:4196943/1‑150 (MQ=255)
           cGCTCAACAACGGTCTGCCAGTACTGCTGTAACGGTGAAGAGAGCGGCTTCATAAGCGATTTTATCCTTGCCTGATTAACGTTTTCCGCTGTGCAACCAGAACGGTTCCTGATTGTTTGCCTCATTACGGAAATGTTCAATTTCGATGCg                                                                                                                                            <  1:2682210/150‑1 (MQ=255)
              tCAACAACGGTCTGCCAGTACTGCTGTAACGGTGAAGAGAGCGGCTTCATAAGCGATTTTATCCTTGCCTGATTAACGTTTTCCGCTGTGCAACCAGAACGGTTCCTGATTGTTTGCCTCATTACGGAAATGTTCAATTTCGATGCGTTg                                                                                                                                         <  1:3261580/150‑1 (MQ=255)
               caacaaCGGTCTGCCAGTACTGCTGTAACGGTGAAGAGAGCGGCTTCATAAGCGATTTTATCCTTGCCTGATTAACGTTTTCCGCTGTGCAACCAGAACGGTTCCTGATTGTTTGCCTCATTACGGAAATGTTCTATTTCGATGCGTTGc                                                                                                                                        <  1:3524236/150‑1 (MQ=255)
               caacaaCGGTCTGCCAGTACTGCTGTAACGGTGAAGAGAGCGGCTTCATAAGCGATTTTATCCTTGCCTGATTAACGTTTTCCGCTGTGCAACCAGAACGGTTCCTGATTGTTTGCCTCATTACGGAAATGTTCTATTTCGATGCGTTGc                                                                                                                                        >  2:387036/1‑150 (MQ=255)
               caacaaCGGTCTGCCAGTACTGCTGTAACGGTGAAGAGAGCGGCTTCATAAGCGATTTTATCCTTGCCTGATTAACGTTTTCCGCTGTGCAACCAGAACGGTTCCTGATTGTTTGCCTCATTACGGAAATGTTCAATTTCGATGCGTTGc                                                                                                                                        <  1:1001798/150‑1 (MQ=255)
               caacaaCGGTCTGCCAGTACTGCTGTAACGGTGAAGAGAGCGGCTTCATAAGCGATTTTATCCTTGCCTGATTAACGTTTTCCGCTGTGCAACCAGAACGGTTCCTGATTGTTTGCCTCATTACGGAAATGTTCAATTTCGATGCGTTGc                                                                                                                                        >  1:755961/1‑150 (MQ=255)
               caacaaCGGTCTGCCAGTACTGCTGTAACGGTGAAGAGAGCGGCTTCATAAGCGATTTTATCCTTGCCTGATTAACGTTTTCCGCTGTGCAACCAGAACGGTTCCTGATTGTTTGCCTCATTACGGAAATGTTCAATTTCGATGCGTTGc                                                                                                                                        <  1:4366573/150‑1 (MQ=255)
               caacaaCGGTCTGCCAGTACTGCTGTAACGGTGAAGAGAGCGGCTTCATAAGCGATTTTATCCTTGCCTGATTAACGTTTTCCGCTGTGCAACCAGAACGGTTCCTGATTGTTTGCCTCATTACGGAAATGTTCAATTTCGATGCGTTGc                                                                                                                                        >  2:2501674/1‑150 (MQ=255)
                aacaacGGTCTGCCAGTACTGCTGTAACGGTGAAGAGAGCGGCTTCATAAGCGATTTTATCCTTGCCTGATTAACGTTTTCCGCTGTGCAACCAGAACGGTTCCTGATTGTTTGCCTCATTACGGAAATGTTCTATTTCGATGCGTTGcc                                                                                                                                       <  2:4500510/150‑1 (MQ=255)
                aacaacGGTCTGCCAGTACTGCTGTAACGGTGAAGAGAGCGGCTTCATAAGCGATTTTATCCTTGCCTGATTAACGTTTTCCGCTGTGCAACCAGAACGGTTCCTGATTGTTTGCCTCATTACGGAAATGTTCAATTTCGATGCGTTGcc                                                                                                                                       >  1:2576299/1‑150 (MQ=255)
                  caacGGTCTGCCAGTACTGCTGTAACGGTGAAGAGAGCGGCTTCATAAGCGATTTTATCCTTGCCTGATTAACGTTTTCCGCTGTGCAACCAGAACGGTTCCTGATTGTTTGCCTCATTACGGAAATGTTCAATTTCGATGCGTTGCCCg                                                                                                                                     >  1:2050117/1‑150 (MQ=255)
                   aacGGTCTGCCAGTACTGCTGTAACGGTGAAGAGAGCGGCTTCATAAGCGATTTTATCCTTGCCTGATTAACGTTTTCCGCTGTGCAACCAGAACGGTTCCTGATTGTTTGCCTCATTACGGAAATGTTCAATTTCGATGCGTTGCCCgg                                                                                                                                    >  2:1881073/1‑150 (MQ=255)
                    acGGTCTGCCAGTACTGCTGTAACGGTGAAGAGAGCGGCTTCATAAGCGATTTTATCCTTGCCTGATTAACGTTTTCCGCTGTGCAACCAGAACGGTTCCTGATTGTTTGCCTCATTACGGAAATGTTCAATTTCGATGCGTTGCCCGGt                                                                                                                                   <  2:1494883/150‑1 (MQ=255)
                     cGGTCTGCCAGTACTGCTGTAACGGTGAAGAGAGCGGCTTCATAAGCGATTTTATCCTTGCCTGATTAACGTTTTCCGCTGTGCAACCAGAACGGTTCCTGATTGTTTGCCTCATTACGGAAATGTTCTATTTCGATGCGTTGCCCGGTc                                                                                                                                  >  2:4096403/1‑150 (MQ=255)
                      ggTCTGCCAGTACTGCTGTAACGGTGAAGAGAGCGGCTTCATAAGCGATTTTATCCTTGCCTGATTAACGTTTTCCGCTGTGCAACCAGAACGGTTCCTGATTGTTTGCCTCATTACGGAAATGTTCAATTTCGATGCGTTGCCCGGTCg                                                                                                                                 <  1:1586496/150‑1 (MQ=255)
                       gTCTGCCAGTACTGCTGTAACGGTGAAGAGAGCGGCTTCATAAGCGATTTTATCCTTGCCTGATTAACGTTTTCCGCTGTGCAACCAGAACGGTTCCTGATTGTTTGCCTCATTACGGAAATGTTCAATTTCGATGCGTTGCCCGGTCGc                                                                                                                                >  2:3243792/1‑150 (MQ=255)
                        tCTGCCAGTACTGCTGTAACGGTGAAGAGAGCGGCTTCATAAGCGATTTTATCCTTGCCTGATTAACGTTTTCCGCTGTGCAACCAGAACGGTTCCTGATTGTTTGCCTCATTACGGAAATGTTCAATTTCGATGCGTTGCCCGGTCGCa                                                                                                                               <  1:28732/150‑1 (MQ=255)
                        tCTGCCAGTACTGCTGTAACGGTGAAGAGAGCGGCTTCATAAGCGATTTTATCCTTGCCTGATTAACGTTGTCCGCTGTGCAACCAGAACGGTTCCTGATTGTTTGCCTCATTACGGAAATGTTCAATTTCGATGCGTTGCCCGGTCGCa                                                                                                                               <  1:1202851/150‑1 (MQ=255)
                          tGCCAGTACTGCTGTAACGGTGAAGAGAGCGGCTTCATAAGCGATTTTATCCTTGCCTGATTAACGTTTTCCGCTGTGCAACCAGAACGGTTCCTGATTGTTTGCCTCATTACGGAAATGTTCAATTTCGATGCGTTGCCCGGTCGCAAt                                                                                                                             <  1:900242/150‑1 (MQ=255)
                              aGTACTGCTGTAACGGTGAAGAGAGCGGCTTCATAAGCGATTTTATCCTTGCCTGATTAACGTTTTCCGCTGTGCAACCAGAACGGTTCCTGATTGTTTGCCTCATTACGGAAATGTTCAATTTCGATGCGTTGCCCGGTCGCAATAGCg                                                                                                                         >  2:4343729/1‑150 (MQ=255)
                               gTACTGCTGTAACGGTGAAGAGAGCGGCTTCATAAGCGATTTTATCCTTGCCTGATTAACGTTTTCCGCTGTGCAACCAGAACGGTTCCTGATTGTTTGCCTCATTACGGAAATGTTCAATTTCGATGCGTTGCCCGGTCGCAATAGCGt                                                                                                                        <  2:1044182/150‑1 (MQ=255)
                                   tgctgTAACGGTGAAGAGAGCGGCTTCATAAGCGATTTTATCCTTGCCTGATTAACGTTTTCCGCTGTGCAACCAGAACGGTTCCTGATTGTTTGCCTCATTACGGAAATGTTCAATTTCGATGCGTTGCCCGGTCGCAATAGCGTGATg                                                                                                                    <  2:1857928/150‑1 (MQ=255)
                                    gctgTAACGGTGAAGAGAGCGGCTTCATAAGCGATTTTATCCTTGCCTGATTAACGTTTTCCGCTGTGCAACCAGAACGGTTCCTGATTGTTTGCCTCATTACGGAAATGTTCAATTTCGATGCGTTGCCCGGTCGCAATAGCGTGATGc                                                                                                                   <  2:1275156/150‑1 (MQ=255)
                                       gTAACGGTGAAGAGAGCGGCTTCATAAGCGATTTTATCCTTGCCTGATTAACGTTTTCCGCTGTGCAACCAGAACGGTTCCTGATTGTTTGCCTCATTACGGAAATGTTCTATTTCGATGCGTTGCCCGGTCGCAATAGCGTGATGCAGc                                                                                                                >  1:446674/1‑150 (MQ=255)
                                        tAACGGTGAAGAGAGCGGCTTCATAAGCGATTTTATCCTTGCCTGATTAACGTTTTCCGCTGTGCAACCAGAACGGTTCCTGATTGTTTGCCTCATTACGGAAATGTTCTATTTCGATGCGTTGCCCGGTCGCAATAGCGTGATGCAGcc                                                                                                               >  1:2193231/1‑150 (MQ=255)
                                          aCGGTGAAGAGAGCGGCTTCATAAGCGATTTTATCCTTGCCTGATTAACGTTTTCCGCTGTGCAACCAGAACGGTTCCTGATTGTTTGCCTCATTACGGAAATGTTCAATTTCGATGCGTTGCCCGGTCGCAATAGCGTGATGCAGcccc                                                                                                             >  1:3101658/1‑150 (MQ=255)
                                           cGGTGAAGAGAGCGGCTTCATAAGCGATTTTATCCTTGCCTGATTAACGTTTTCCGCTGTGCAACCAGAACGGTTCCTGATTGTTTGCCTCATTACGGAAATGTTCTATTTCGATGCGTTGCCCGGTCGCAATAGCGTGATGCAGCCCCt                                                                                                            >  2:1336440/1‑150 (MQ=255)
                                            ggTGAAGAGAGCGGCTTCATAAGCGATTTTATCCTTGCCTGATTAACGTTTTCCGCTGTGCAACCAGAACGGTTCCTGATTGTTTGCCTCATTACGGAAATGTTCAATTTCGATGCGTTGCCCGGTCGCAATAGCGTGATGCAGCCCCTg                                                                                                           <  2:3134103/150‑1 (MQ=255)
                                              tGAAGAGAGCGGCTTCATAAGCGATTTTATCCTTGCCTGATTAACGTTTTCCGCTGTGCAACCAGAACGGTTCCTGATTGTTTGCCTCATTACGGAAATGTTCAATTTCGATGCGTTGCCCGGTCGCAATAGCGTGATGCAGCCCCTGcc                                                                                                         <  2:208380/150‑1 (MQ=255)
                                               gAAGAGAGCGGCTTCATAAGCGATTTTATCCTTGCCTGATTAACGTTTTCCGCTGTGCAACCAGAACGGTTCCTGATTGTTTGCCTCATTACGGAAATGTTCAATTTCGATGCGTTGCCCGGTCGCAATAGCGTGATGCAGCCCCTGCCa                                                                                                        <  1:2117156/150‑1 (MQ=255)
                                                  gagagCGGCTTCATAAGCGATTTTATCCTTGCCTGATTAACGTTTTCCGCTGTGCAACCAGAACGGTTCCTGATTGTTTGCCTCATTACGGAAATGTTCAATTTCGATGCGTTGCCCGGTCGCAATAGCGTGATGCAGCCCCTGCCAGtt                                                                                                     <  1:2113700/150‑1 (MQ=255)
                                                   agagCGGCTTCATAAGCGATTTTATCCTTGCCTGATTAACGTTTTCCGCTGTGCAACCAGAACGGTTCCTGATTGTTTGCCTCATTACGGAAATGTTCAATTTCGATGCGTTGCCCGGTCGCAATAGCGTGATGCAGCCCCTGCCAGTtc                                                                                                    >  1:476196/1‑150 (MQ=255)
                                                             cATCAGCGATTTTATCCTTGCCTGATTAACGTTTTCCGCTGTGCAACCAGAACGGTTCCTGATTGTTTGCCTCATTACGGAAATGTTCAATTTCGATGCGTTGCCCGGTCGCAATAGCGTGATGCAGCCCCTGCCAGTTCTCCAGCCAgg                                                                                          <  2:1013483/150‑1 (MQ=255)
                                                             cATAAGCGATTTTATCCTTGCCTGATTAACGTTTTCCGCTGTGCAACCAGAACGGTTCCTGATTGTTTGCCTCATTACGGAAATGTTCAATTTCGATGCGTTGCCCGGTCGCAATAGCGTGATGCAGCCCCTGCCAGTTCTCCAGCCAgg                                                                                          >  2:615623/1‑150 (MQ=255)
                                                              aTAAGCGATTTTATCCTTGCCTGATTAACGTTTTCCGCTGTGCAACCAGAACGGTTCCTGATTGTTTGCCTCATTACGGAAATGTTCAATTTCGATGCGTTGCCCGGTCGCAATAGCGTGATGCAGCCCCTGCCAGTTCTCCAGCCAGGc                                                                                         >  1:2511793/1‑150 (MQ=255)
                                                                 aGCGATTTTATCCTTGCCTGATTAACGTTTTCCGCTGTGCAACCAGAACGGTTCCTGATTGTTTGCCTCATTACGGAAATGTTCAATTTCGATGCGTTGCCCGGTCGCAATAGCGTGATGCAGCCCCTGCCAGTTCTCCAGCCAGGCTTg                                                                                      >  2:2561716/1‑150 (MQ=255)
                                                                          aTCCTTGCCTGATTAACGTTTTCCGCTGTGCAACCAGAACGGTTCCTGATTGTTTGCCTCATTACGGAAATGTTCAATTTCGATGCGTTGCCCGGTCGCAATAGCGTGATGCAGCCCCTGCCAGTTCTCCAGCCAGGCTTGCGCgacgac                                                                             <  2:628575/150‑1 (MQ=255)
                                                                             cTTGCCTGATTAACGTTTTCCGCTGTGCAACCAGAACGGTTCCTGATTGTTTGCCTCATTACGGAAATGTTCTATTTCGATGCGTTGCCCGGTCGCAATAGCGTGATGCAGCCCCTGCCAGTTCTCCAGCCAGGCTTGCGCGACGACAgg                                                                          >  1:1992143/1‑150 (MQ=255)
                                                                               tGCCTGATTAACGTTTTCCGCTGTGCAACCAGAACGGTTCCTGATTGTTTGCCTCATTACGGAAATGTTCAATTTCGATGCGTTGCCCGGTCGCAATAGCGTGATGCAGCCCCTGCCAGTTCTCCAGCCAGGCTTGCGCGACGACAGGAt                                                                        >  2:4216441/1‑150 (MQ=255)
                                                                                 ccTGATTAACGTTTTCCGCTGTGCAACCAGAACGGTTCCTGATTGTTTGCCTCATTACGGAAATGTTCAATTTCGATGCGTTGCCCGGTCGCAATAGCGTGATGCAGCCCCTGCCAGTTCTCCAGCCAGGCTTGCGCGACGACAGGATCa                                                                      <  1:3473655/150‑1 (MQ=255)
                                                                                  cTGATTAACGTTTTCCGCTGTGCAACCAGAACGGTTCCTGATTGTTTGCCTCATTACGGAAATGTTCAATTTCGATGCGTTGCCCGGTCGCAATAGCGTGATGCAGCCCCTGCCAGTTCTCCAGCCAGGCTTGCGCGACGACAGGATCAt                                                                     <  1:3697570/150‑1 (MQ=255)
                                                                                   tGATTAACGTTTTCCGCTGTGCAACCAGAACGGTTCCTGATTGTTTGCCTCATTACGGAAATGTTCAATTTCGATGCGTTGCCCGGTCGCAATAGCGTGATGCAGCCCCTGCCAGTTCTCCAGCCAGGCTTGCGCGACGACAGGATCATa                                                                    >  2:1323691/1‑150 (MQ=255)
                                                                                    gATTAACGTTTTCCGCTGTGCAACCAGAACGGTTCCTGATTGTTTGCCTCATTACGGAAATGTTCAATTTCGATGCGTTGCCCGGTCGCAATAGCGTGATGCAGCCCCTGCCAGTTCTCCAGCCAGGCTTGCGCGACGACAGGATCATAg                                                                   >  1:4202142/1‑150 (MQ=255)
                                                                                    gATTAACGTTTTCCGCTGTGCAACCAGAACGGTTCCTGATTGTTTGCCTCATTACGGAAATGTTCAATTTCGATGCGTTGCCCGGTCGCAATAGCGTGATGCAGCCCCTGCCAGTTCTCCAGCCAGGCTTGCGCGACGACAGGATCATAg                                                                   >  1:1927501/1‑150 (MQ=255)
                                                                                      ttAAAGTTTTCCGCTGTGCAACCAGAACGGTTCCTGATTGTTTGCCTCATTACGGAAATGTTCAATTTCGATGCGTTGCCCGGTCGCAATAGCGTGATGCAGCCCCTGCCAGTTCTCCAGCCAGGCTTGCGCGACGACAGGATCATAGTa                                                                 <  1:3984060/150‑1 (MQ=255)
                                                                                        aaCGTTTTCCGCTGTGCAACCAGAACGGTTCCTGATTGTTTGCCTCATTACGGAAATGTTCAATTTCGATGCGTTGCCCGGTCGCAATAGCGTGATGCAGCCCCTGCCAGTTCTCCAGCCAGGCTTGCGCGACGACAGGATCATAGTAAc                                                               >  1:2343780/1‑150 (MQ=255)
                                                                                         aCGTTTTCCGCTGTGCAACCAGAACGGTTCCTGATTGTTTGCCTCATTACGGAAATGTTCAATTTCGATGCGTTGCCCGGTCGCAATAGCGTGATGCAGCCCCTGCCAGTTCTCCAGCCAGGCTTGCGCGACGACAGGATCATAGTAAcc                                                              <  1:2636785/150‑1 (MQ=255)
                                                                                          cGTTTTCCGCTGTGCAACCAGAACGGTTCCTGATTGTTTGCCTCATTACGGAAATGTTCAATTTCGATGCGTTGCCCGGTCGCAATAGCGTGATGCAGCCCCTGCCAGTTCTCCAGCCAGGCTTGCGCGACGACAGGATCATAGTAAccc                                                             >  1:1881068/1‑150 (MQ=255)
                                                                                           gTTTTCCGCTGTGCAACCAGAACGGTTCCTGATTGTTTGCCTCATTACGGAAATGTTCAATTTCGATGCGTTGCCCGGTCGCAATAGCGTGATGCAGCCCCTGCCAGTTCTCCAGCCAGGCTTGCGCGACGACAGGATCATAGTAACCCg                                                            >  2:3347251/1‑150 (MQ=255)
                                                                                           gTTTTCCGCTGTGCAACCAGAACGGTTCCTGATTGTTTGCCTCATTACGGAAATGTTCAATTTCGATGCGTTGCCCGGTCGCAATAGCGTGATGCAGCCCCTGCCAGTTCTCCAGCCAGGCTTGCGCGACGACAGGATCATAGTAACCCg                                                            >  1:1078750/1‑150 (MQ=255)
                                                                                           gTTTTCCGCTGAGCAACCAGAACGGTTCCTGATTGTTTGCCTCATTACGGAAATGTTCTATTTCGATGCGTTGCCCGGTCGCAATAGCGTGATGCAGCCCCTGCCAGTTCTCCAGCCAGGCTTGCGCGACGACAGGATCATAGTAACCCg                                                            >  2:3895865/1‑150 (MQ=255)
                                                                                              ttCCGCTGTGCAACCAGAACGGTTCCTGATTGTTTGCCTCATTACGGAAATGTTCAATTTCGATGCGTTGCCCGGTCGCAATAGCGTGATGCAGCCCCTGCCAGTTCTCCAGCCAGGCTTGCGCGACGACAGGATCATAGTAACCCGCCa                                                         >  2:1531071/1‑150 (MQ=255)
                                                                                              ttCCGCTGTGCAACCAGAACGGTTCCTGATTGTTTGCCTCATTACGGAAATGTTCAATTTCGATGCGTTGCCCGGTCGCAATAGCGTGATGCAGCCCCTGCCAGTTCTCCAGCCAGGCTTGCGCGACGACAGGATCATAGTAACCCGCCa                                                         <  1:1948324/150‑1 (MQ=255)
                                                                                                ccGCTGTGCAACCAGAACGGTTCCTGATTGTTTGCCTCATTACGGAAATGTTCAATTTCGATGCGTTGCCCGGTCGCAATAGCGTGATGCAGCCCCTGCCAGTTCTCCAGCCAGGCTTGCGCGACGACAGGATCATAGTAACCCGCCAGc                                                       >  2:1621950/1‑150 (MQ=255)
                                                                                                   cTGTGCAACCAGAACGGTTCCTGATTGTTTGCCTCATTACGGAAATGTTCAATTTCGATGCGTTGCCCGGTCGCAATAGCGTGATGCAGCCCCTGCCAGTTCTCCAGCCAGGCTTGCGCGACGACAGGATCATAGTAACCCGCCAGCAAc                                                    >  1:297457/1‑150 (MQ=255)
                                                                                                   cTGTGCAACCAGAACGGTTCCTGATTGTTTGCCTCATTACGGAAATGTTCAATTTCGATGCGTTGCCCGGTCGCAATAGCGTGATGCAGCCCCTGCCAGTTCTCCAGCCAGGCTTGCGAGACGACAGGATCATAGTAACCCGCCAGCAAc                                                    >  2:3668699/1‑150 (MQ=255)
                                                                                                     gtgCAACCAGAACGGTTCCTGATTGTTTGCCTCATTACGGAAATGTTCAATTTCGATGCGTTGCCCGGTCGCAATAGCGTGATGCAGCCCCTGCCAGTTCTCCAGCCAGGCTTGCGCGACGACAGGATCATAGTAACCCGCCAGCAACAg                                                  <  1:2774208/150‑1 (MQ=255)
                                                                                                            cAGAACGGTTCCTGATTGTTTGCCTCATTACGGAAATGTTCAATTTCGATGCGTTGCCCGGTCGCAATAGCGTGATGCAGCCCCTGCCAGTTCTCCAGCCAGGCTTGCGCGACGACAGGATCATAGTAACCCGCCAGCAACAGTATTGAg                                           <  2:2397850/150‑1 (MQ=255)
                                                                                                             aGAACGGTTCCTGATTGTTTGCCTCATTACGGAAATGTTCTATTTCGATGCGTTGCCCGGTCGCAATAGCGTGATGCAGCCCCTGCCAGTTCTCCAGCCAGGCTTGCGCGACGACAGGATCATAGTAACACGCCAGCAACAGTATTGAGt                                          >  1:170271/1‑150 (MQ=255)
                                                                                                              gAACGGTTCCTGATTGTTTGCCTCATTACGGAAATGTTCAATTTCGATGCGTTGCCCGGTCGCAATAGCGTGATGCAGCCCCTGCCAGTTCTCCAGCCAGGCTTGCGCGACGACAGGATCATAGTAACCCGCCAGCAACAGTATTGAGTc                                         >  1:4261775/1‑150 (MQ=255)
                                                                                                              gAACGGTTCCTGATTGTTTGCCTCATTACGGAAATGTTCAATTTCGATGCGTTGCCCGGTCGCAATAGCGTGATGCAGCCCCTGCCAGTTCTCCAGCCAGGCTTGCGCGACGACAGGATCATAGTAACCCGCCAGCAACAGTATTGAGTc                                         >  1:3885435/1‑150 (MQ=255)
                                                                                                                aCGGTTCCTGATTGTTTGCCTCATTACGGAAATGTTCAATTTCGATGCGTTGCCCGGTCGCAATAGCGTGATGCAGCCCCTGCCAGTTCTCCAGCCAGGCTTGCGCGACGACAGGATCATAGTAACCCGCCAGCAACAGTATTGAGTCaa                                       >  2:3006931/1‑150 (MQ=255)
                                                                                                                aCGGTTCCTGATTGTTTGCCTCATTACGGAAATGTTCAATTTCGATGCGTTGCCCGGTCGCAATAGCGTGATGCAGCCCCTGCCAGTTCTCCAGCCAGGCTTGCGCGACGACAGGATCATAGTAACCCGCCAGCAACAGTATTGAGTCaa                                       >  2:1701159/1‑150 (MQ=255)
                                                                                                                  ggTTCCTGATTGTTTGCCTCATTACGGAAATGTTCTATTTCGATGCGTTGCCCGGTCGCAATAGCGTGATGCAGCCCCTGCCAGTTCTCCAGCCAGGCTTGCGCGACGACAGGATCATAGTAACCCGCCAGCAACAGTATTGAGTCAata                                     <  1:3035676/150‑1 (MQ=255)
                                                                                                                  ggTTCCTGATTGTTTGCCTCATTACGGAAATGTTCAATTTCGATGCGTTGCCCGGTCGCAATAGCGTGATGCAGCCCCTGCCAGTTCTCCAGCCAGGCTTGCGCGACGACAGGATCATAGTAACCCGCCAGCAACAGTATTGAGTCAata                                     >  1:3547997/1‑150 (MQ=255)
                                                                                                                   gTTCCTGATTGTTTGCCTCATTACGGAAATGTTCAATTTCGATGCGTTGCCCGGTCGCAATAGCGTGATGCAGCCCCTGCCAGTTCTCCAGCCAGGCTTGCGCGACGACAGGATCATAGTAACCCGCCAGCAACAGTATTGAGTCAatat                                    <  1:4338016/150‑1 (MQ=255)
                                                                                                                    ttCCTGATTGTTTGCCTCATTACGGAAATGTTCAATTTCGATGCGTTGCCCGGTCGCAATAGCGTGATGCAGCCCCTGCCAGTTCTCCAGCCAGGCTTGCGCGACGACAGGATCATAGTAACCCGCCAGCAACAGTATTGAGTCAATATc                                   >  2:3838365/1‑150 (MQ=255)
                                                                                                                    ttCCTGATTGTTTGCCTCATTACGGAAATGTTCAATTTCGATGCGTTGCCCGGTCGCAATAGCGTGATGCAGCCCCTGCCAGTTCTCCAGCCAGGCTTGCGCGACGACAGGATCATAGTAACCCGCCAGCAACAGTATTGAGTCAATATc                                   >  1:1164850/1‑150 (MQ=255)
                                                                                                                    ttCCTGATTGTTTGCCTCATTACGGAAATGTTCAATTTCGATGCGTTGCCCGGTCGCAATAGCGTGATGCAGCCCCTGCCAGTTCTCCAGCCAGGCTTGCGCGACGACAGGATCATAGTAACCCGCCAGCAACAGTATTGAGTCAATATc                                   >  2:1593242/1‑150 (MQ=255)
                                                                                                                     tCCTGATTGTTTGCCTCATTACGGAAATGTTCAATTTCGATGCGTTGCCCGGTCGCAATAGCGTGATGCAGCCCCTGCCAGTTCTCCAGCCAGGCTTGCGCGACGACAGGATCATAGTAACCCGCCAGCAACAGTATTGAGTCAATATCa                                  <  1:333343/150‑1 (MQ=255)
                                                                                                                      ccTGATTGTTTGCCTCATTACGGAAATGTTCAATTTCGATGCGTTGCCCGGTCGCAATAGCGTGATGCAGCCCCTGCCAGTTCTCCAGCCAGGCTTGCGCGACGACAGGATCATAGTAACCCGCCAGCAACAGTATTGAGTCAATATCAc                                 >  2:2802697/1‑150 (MQ=255)
                                                                                                                       cTGATTGTTTGCCTCATTACGGAAATGTTCAATTTCGATGCGTTGCCCGGTCGCAATAGCGTGATGCAGCCCCTGCCAGTTCTCCAGCCAGGCTTGCGCGACGACAGGATCATAGTAACCCGCCAGCAACAGTATTGAGTCAATATCAcg                                >  1:3187982/1‑150 (MQ=255)
                                                                                                                       cTGATTGTTTGCCTCATTACGGAAATGTTCAATTTCGATGCGTTGCCCGGTCGCAATAGCGTGATGCAGCCCCTGCCAGTTCTCCAGCCAGGCTTGCGCGACGACAGGATCATAGTAACCCGCCAGCAACAGTATTGAGTCAATATCAcg                                <  2:1968752/150‑1 (MQ=255)
                                                                                                                        tGATTGTTTGCCTCATTACGGAAATGTTCAATTTCGATGCGTTGCCCGGTCGCAATAGCGTGATGCAGCCCCTGCCAGTTCTCCAGCCAGGCTTGCGCGACGACAGGATCATAGTAACCCGCCAGCAACAGTATTGAGTCAATATCAcgc                               >  2:1176574/1‑150 (MQ=255)
                                                                                                                          aTTGTTTGCCTCATTACGGAAATGTTCAATTTCGATGCGTTGCCCGGTCGCAATAGCGTGATGCAGCCCCTGCCAGTTCTCCAGCCAGGCTTGCGCGACGACAGGATCATAGTAACCCGCCAGCAACAGTATTGAGTCAATATCACGCGt                             >  1:1299981/1‑150 (MQ=255)
                                                                                                                            tGTTTGCCTCATTACGGAAATGTTCTATTTCGATGCGTTGCCCGGTCGCAATAGCGTGATGCAGCCCCTGCCAGTTCTCCAGCCAGGCTTGCGCGACGACAGGATCATAGTAACCCGCCAGCAACAGTATTGAGTCAATATCACGCGTCa                           <  2:3935500/150‑1 (MQ=255)
                                                                                                                             gTTTGCCTCATTACGGAAATGTTCAATTTCGATGCGTTGCCCGGTCGCAATAGCGTGATGCAGCCCCTGCCAGTTCTCCAGCCAGGCTTGCGCGACGACAGGATCATAGTAACCCGCCAGCAACAGTATTGAGTCAATATCACGCGTCAg                          >  2:178526/1‑150 (MQ=255)
                                                                                                                                tGCCTCATTACGGAAATGTTCAATTTCGATGCGTTGCCCGGTCGCAATAGCGTGATGCAGCCCCTGCCAGTTCTCCCGCCAGGCTGGCGCGACGACAGGATCCTAGTAACCCGCCCGCACCAGTATTGAGGCAATATCACGCGTCAGGCg                       >  2:3462518/1‑150 (MQ=255)
                                                                                                                                  ccTCATTACGGAAATGTTCAATTTCGATGCGTTGCCCGGTCGCAATAGCGTGATGCAGCCCCTGCCAGTTCTCCAGCCAGGCTTGCGCGACGACAGGATCATAGTAACCCGCCAGCAACAGTATTGAGTCAATATCACGCGTCAGGCGTg                     <  1:4041722/150‑1 (MQ=255)
                                                                                                                                  ccTCATTACGGAAATGTTCAATTTCGATGCGTTGCCCGGTCGCAATAGCGTGATGCAGCCCCTGCCAGTTCTCCAGCCAGGCTTGCGCGACGACAGGATCATAGTAACCCGCCAGCAACAGTATTGAGTCAATATCACGCGTCAGGCGTg                     <  1:2301819/150‑1 (MQ=255)
                                                                                                                                   cTCATTACGGAAATGTTCAATTTCGATGCGTTGCCCGGTCGCAATAGCGTGATGCAGCCCCTGCCAGTTCTCCAGCCAGGCTTGCGCGACGACAGGATCATAGTAACCCGCCAGCAACAGTATTGAGTCAATATCACGCGTCAGGCGTgg                    <  2:2298924/150‑1 (MQ=255)
                                                                                                                                     aaTTACGGAAATGTTCTATTTCGATGCGTTGCCCGGTCGCAATAGCGTGATGCAGCCCCTGCCAGTTCTCCAGCCAGGCTTGCGCGACGACAGGATCATAGTAACCCGCCAGCAACAGTATTGAGTCAATATCACGCGTCAGGCGTGGCa                  >  1:1816699/2‑150 (MQ=255)
                                                                                                                                      aTTACGGAAATGTTCAATTTCGATGCGTTGCCCGGTCGCAATAGCGTGATGCAGCCCCTGCCAGTTCTCCAGCCAGGCTTGCGCGACGACAGGATCATAGTAACCCGCCAGCAACAGTATTGAGTCAATATCACGCGTCAGGCGTGGCaa                 >  2:2643327/1‑150 (MQ=255)
                                                                                                                                         aCGGAAATGTTCAATTTCGATGCGTTGCCCGGTCGCAATAGCGTGATGCAGCCCCTGCCAGTTCTCCAGCCAGGCTTGCGCGACGACAGGATCATAGTAACCCGCCAGCAACAGTATTGAGTCAATATCACGCGTCAGGCGTGGCAACTg              >  1:1243206/1‑150 (MQ=255)
                                                                                                                                         aCGGAAATGTTCAATTTCGATGCGTTGCCCGGTCGCAATAGCGTGATGCAGCCCCTGCCAGTTCTCCAGCCAGGCTTGCGCGACGACAGGATCATAGTAACCCGCCAGCAACAGTATTGAGTCAATATCACGCGTCAGGCGTGGCAACTg              >  1:2674390/1‑150 (MQ=255)
                                                                                                                                         aCGGAAATGTTCAATTTCGATGCGTTGCCCGGTCGCAATAGCGTGATGCAGCCCCTGCCAGTTCTCCAGCCAGGCTTGCGCGACGACAGGATCATAGTAACCCGCCAGCAACAGTATTGAGTCAATATCACGCGTCAGGCGTGGCAACTg              <  1:4076889/150‑1 (MQ=255)
                                                                                                                                           ggAAATGTTCAATTTCGATGCGTTGCCCGGTCGCAATAGCGTGATGCAGCCCCTGCCAGTTCTCCAGCCAGGCTTGCGCGACGACAGGATCATAGTAACCCGCCAGCAACAGTATTGAGTCAATATCACGCGTCAGGCGTGGCAACTGGt            >  1:590539/1‑150 (MQ=255)
                                                                                                                                            gAAATGTTCAATTTCGATGCGTTGCCCGGTCGCAATAGCGTGATGCAGCCCCTGCCAGTTCTCCAGCCAGGCTTGCGCGACGACAGGATCATAGTAACCCGCCAGCAACAGTATTGAGTCAATATCACGCGTCAGGCGTGGCAACTGGTc           >  2:4118584/1‑150 (MQ=255)
                                                                                                                                               aTGTTCAATTTCGATGCGTTGCCCGGTCGCAATAGCGTGATGCAGCCCCTGCCAGTTCTCCAGCCAGGCTTGCGCGACGACAGGATCATAGTAACCCGCCAGCAACAGTATTGAGTCAATATCACGCGTCAGGCGTGGCAACTGGTCACg        >  2:856717/1‑150 (MQ=255)
                                                                                                                                                tGTTCAATTTCGATGCGTTGCCCGGTCGCAATAGCGTGATGCAGCCCCTGCCAGTTCTCCAGCCAGGCTTGCGCGACGACAGGATCATAGTAACCCGCCAGCAACAGTATTGAGTCAATATCACGCGTCAGGCGTGGCAACTGGTCACgg       >  2:114710/1‑150 (MQ=255)
                                                                                                                                                 gTTCAATTTCGATGCGTTGCCCGGTCGCAATAGCGTGATGCAGCCCCTGCCAGTTCTCCAGCCAGGCTTGCGCGACGACAGGATCATAGTAACCCGCCAGCAACAGTATTGAGTCAATATCACGCGTCAGGCGTGGCAACTGGTCACGGt      >  2:769403/1‑150 (MQ=255)
                                                                                                                                                    cAATTTCGATGCGTTGCCCGGTCGCAATAGCGTGATGCAGCCCCTGCCAGTTCTCCAGCCAGGCTTGCGCGACGACAGGATCATAGTAACCCGCCAGCAACAGTATTGAGTCAATATCACGCGTCAGGCGTGGCAACTGGTCACGGTAGc   >  2:2758417/1‑150 (MQ=255)
                                                                                                                                                     aaTTTCGATGCGTTGCCCGGTCGCAATAGCGTGATGCAGCCCCTGCCAGTTCTCCAGCCAGGCTTGCGCGACGACAGGATCATAGTAACCCGCCAGCAACAGTATTGAGTCAATATCACGCGTCAGGCGTGGCAACTGGTCACGGTAGCg  >  1:3244787/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
GCTCTGGCAGCCGCTCAACAACGGTCTGCCAGTACTGCTGTAACGGTGAAGAGAGCGGCTTCATAAGCGATTTTATCCTTGCCTGATTAACGTTTTCCGCTGTGCAACCAGAACGGTTCCTGATTGTTTGCCTCATTACGGAAATGTTCAATTTCGATGCGTTGCCCGGTCGCAATAGCGTGATGCAGCCCCTGCCAGTTCTCCAGCCAGGCTTGCGCGACGACAGGATCATAGTAACCCGCCAGCAACAGTATTGAGTCAATATCACGCGTCAGGCGTGGCAACTGGTCACGGTAGCG  >  CP009273/3192937‑3193235

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑