Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,204,711 C→A 5.2% F82L (TTC→TTA)  dnaG → DNA primase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,204,7110CA 5.2% 1150.6 / 26.1 302F82L (TTC→TTA) dnaGDNA primase
Reads supporting (aligned to +/- strand):  ref base C (144/141);  new base A (9/8);  total (153/149)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 320 total aligned reads displayed.

GGCAAGAATTTCCACGCGTGTTGTCCATTCCACAACGAGAAAACCCCGTCCTTCACCGTTAACGGTGAGAAACAGTTTTACCACTGCTTTGGATGTGGCGCGCACGGCAACGCGATCGACTTCCTGATGAACTACGACAAGCTCGAGTTCGTCGAAACGGTCGAAGAGCTGGCAGCAATGCACAATCTTGAAGTGCCATTTGAAGCAGGCAGCGGCCCCAGCCAGATCGAGCGCCATCAGAGGCAAACGCTTTATCAGTTGATGGACGGTCTGAATACGTTTTACCAACAATCTTTACA  >  CP009273/3204562‑3204860
                                                                                                                                                     |                                                                                                                                                     
ggCAAGAATTTCCACGCGTGTTGTCCATTCCACAACGAGAAAACCCCGTCCTTCACCGTTAACGGTGAGAAACAGTTTTACCACTGCTTTGGATGTGGCGCGCACGGCAACGCGATCGACTTCCTGATGAACTACGACAAGCTCGAGTtc                                                                                                                                                       <  1:2371563/150‑1 (MQ=255)
          tCCACGCGTGTTGTCCATTCCACAACGAGAAAACCCCGTCCTTCACCGTTAACGGTGAGAAACAGTTTTACCACTGCTTTGGATGTGGCGCGCACGGCAACGCGATCGACTTCCTGATGAACTACGACAAGCTCGAGTTCGTCGAAACgg                                                                                                                                             >  2:3001762/1‑150 (MQ=255)
          tCCACGCGTGTTGTCCATTCCACAACGAGAAAACCCCGTCCTTCACCGTTAACGGTGAGAAACAGTTTTACCACTGCTTTGGATGTGGCGCGCACGGCAACGCGATCGACTTCCTGATGAACTACGACAAGCTCGAGTTCGTCGAAACgg                                                                                                                                             >  1:2427396/1‑150 (MQ=255)
            cACGCGTGTTGTCCATTCCACAACGAGAAAACCCCGTCCTTCACCGTTAACGGTGAGAAACAGTTTTACCACTGCTTTGGATGTGGCGCGCACGGCAACGCGATCGACTTCCTGATGAACTACGACAAGCTCGAGTTCGTCGAAACGGTc                                                                                                                                           >  2:4513630/1‑150 (MQ=255)
             aCGCGTGTTGTCCATTCCACAACGAGAAAACCCCGTCCTTCACCGTTAACGGTGAGAAACAGTTTTACCACTGCTTTGGATGTGGCGCGCACGGCAACGCGATCGACTTCCTGATGAACTACGACAAGCTCGAGTTCGTCGAAACGGTCg                                                                                                                                          <  2:424893/150‑1 (MQ=255)
             aCGCGTGTTGTCCATTCCACAACGAGAAAACCCCGTCCTTCACCGTTAACGGTGAGAAACAGTTTTACCACTGCTTTGGATGTGGCGCGCACGGCAACGCGATCGACTTCCTGATGAACTACGACAAGCTCGAGTTCGTCGAAACGGTCg                                                                                                                                          >  1:1373791/1‑150 (MQ=255)
              cgcgTGTTGTCCATTCCACAACGAGAAAACCCCGTCCTTCACCGTTAACGGTGAGAAACAGTTTTACCACTGCTTTGGATGTGGCGCGCACGGCAACGCGATCGACTTCCTGATGAACTACGACAAGCTCGAGTTCGTCGAAACGGTCGa                                                                                                                                         <  2:2142783/150‑1 (MQ=255)
                cgTGTTGTCCATTCCACAACGAGAAAACCCCGTCCTTCACCGTTAACGGTGAGAAACAGTTTTACCACTGCTTTGGATGTGGCGCGCACGGCAACGCGATCGACTTCCTGATGAACTACGACAAGCTCGAGTTCGTCGAAACGGTCGAag                                                                                                                                       <  2:847700/150‑1 (MQ=255)
                cgTGTTGTCCATTCCACAACGAGAAAACCCCGTCCTTCACCGTTAACGGTGAGAAACAGTTTTACCACTGCTTTGGATGTGGCGCGCACGGCAACGCGATCGACTTCCTGATGAACTACGACAAGCTCGAGTTCGTCGAAACGGTCGAag                                                                                                                                       <  2:3892743/150‑1 (MQ=255)
                 gtgtTGTCCATTCCACAACGAGAAAACCCCGTCCTTCACCGTTAACGGTGAGAAACAGTTTTACCACTGCTTTGGATGTGGCGCGCACGGCAACGCGATCGACTTTCTGATGAACTACGACAAGCTCGAGTTCGTCGAAACGGTCGAaga                                                                                                                                      >  2:4331086/1‑150 (MQ=255)
                   gttgtCCATTCCACAACGAGAAAACCCCGTCCTTCACCGTTAACGGTGAGAAACAGTTTTACCACTGCTTTGGATGTGGCGCGCACGGCAACGCGATCGACTTCCTGATGAACTACGACAAGCTCGAGTTCGTCGAAACGGTCGAAGAGc                                                                                                                                    >  1:886836/1‑150 (MQ=255)
                    ttgtCCATTCCACAACGAGAAAACCCCGTCCTTCACCGTTAACGGTGAGAAACAGTTTTACCACTGCTTTGGATGTGGCGCGCACGGCAACGCGATCGACTTCCTGATGAACTACGACAAGCTCGAGTTCGTCGAAACGGTCGAAGAGCt                                                                                                                                   <  2:3516554/150‑1 (MQ=255)
                     tgtCCATTCCACAACGAGAAAACCCCGTCCTTCACCGTTAACGGTGAGAAACAGTTTTACCACTGCTTTGGATGTGGCGCGCACGGCAACGCGATCGACTTCCTGATGAACTACGACAAGCTCGAGTTCGTCGAAACGGTCGAAGAGCTg                                                                                                                                  >  1:3343527/1‑150 (MQ=255)
                      gtCCATTCCACAACGAGAAAACCCCGTCCTTCACCGTTAACGGTGAGAAACAGTTTTACCACTGCTTTGGATGTGGCGCGCACGGCAACGCGATCGACTTTCTGATGAACTACGACAAGCTCGAGTTCGTCGAAACGGTCGAAGAGCTgg                                                                                                                                 <  2:4458099/150‑1 (MQ=255)
                       tCCATTCCACAACGAGAAAACCCCGTCCTTCACCGTTAACGGTGAGAAACAGTTTTACCACTGCTTTGGATGTGGCGCGCACGGCAACGCGATCGACTTCCTGATGAACTACGACAAGCTCGAGTTCGTCGAAACGGTCGAAGAGCTGgc                                                                                                                                <  2:2394288/150‑1 (MQ=255)
                        ccATTCCACAACGAGAAAACCCCGTCCTTCACCGTTAACGGTGAGAAACAGTTTTACCACTGCTTTGGATGTGGCGCGCACGGCAACGCGATCGACTTTCTGATGAACTACGACAAGCTCGAGTTCGTCGAAACGGTCGAAGAGCTGgca                                                                                                                               >  1:1263025/1‑150 (MQ=255)
                        ccATTCCACAACGAGAAAACCCCGTCCTTCACCGTTAACGGTGAGAAACAGTTTTACCACTGCTTTGGATGTGGCGCGCACGGCAACGCGATCGACTTCCTGATGAACTACGACAAGCTCGAGTTCGTCGAAACGGTCGAAGAGCTGgca                                                                                                                               <  1:1532008/150‑1 (MQ=255)
                        ccATTCCACAACGAGAAAACCCCGTCCTTCACCGTTAACGGTGAGAAACAGTTTTACAACTGCTTTGGATGTGGCTCGCACTGCAACGCGATCGACTTCCTGATGAACTACGACAAGCTCGAGTTCGTCGAAACGGTCGAAGAGCTGgca                                                                                                                               <  1:1992842/150‑1 (MQ=255)
                         cATTCCACAACGAGAAAACGCCGTCCTTCACCGTTAACGGTGAGAAACAGTTTTACCACTGCTTTGGATGTGGCGCGCACGGCAACGCGATCGACTTCCTGATGAACTACGACAAGCTCGAGTTCGTCGAAACGGTCGAAGAGCTGgcag                                                                                                                              <  2:4065610/150‑1 (MQ=255)
                          aTTCCACAACGAGAAAACCCCGTCCTTCACCGTTAACGGTGAGAAACAGTTTTACCACTGCTTTGGATGTGGCGCGCACGGCAACGCGATCGACTTCCTGATGAACTACGACAAGCTCGAGTTCGTCGAAACGGTCGAAGAGCTGgcagc                                                                                                                             >  1:729641/1‑150 (MQ=255)
                            tCCACAACGAGAAAACCCCGTCCTTCACCGTTAACGGTGAGAAACAGTTTTACCACTGCTTTGGATGTGGCGCGCACGGCAACGCGATCGACTTCCTGATGAACTACGACAAGCTCGAGTTCGTCGAAACGGTCGAAGAGCTGGCAGCaa                                                                                                                           >  2:1656397/1‑150 (MQ=255)
                              cacaACGAGAAAACCCCGTCCTTCACCGTTAACGGTGAGAAACAGTTTTACCACTGCTTTGGATGTGGCGCGCACGGCAACGCGATCGACTTCCTGATGAACTACGACAAGCTCGAGTTCGTCGAAACGGTCGAAGAGCTGGCAGCAATg                                                                                                                         >  1:3818024/1‑150 (MQ=255)
                                 aaCGAGAAAACCCCGTCCTTCACCGTTAACGGTGAGAAACAGTTTTACCACTGCTTTGGATGTGGCGCGCACGGCAACGCGATCGACTTCCTGATGAACTACGACAAGCTCGAGTTCGTCGAAACGGTCGAAGAGCTGGCAGCAATGcac                                                                                                                      <  2:1897323/150‑1 (MQ=255)
                                 aaCGAGAAAACCCCGTCCTTCACCGTTAACGGTGAGAAACAGTTTTACCACTGCTTTGGATGTGGCGCGCACGGCAACGCGATCGACTTCCTGATGAACTACGACAAGCTCGAGTTAGTCGAAACGGTCGAAGAGCTGGCAGCAATGcac                                                                                                                      >  2:3262600/1‑150 (MQ=255)
                                   cGAGAAAACCCCGTCCTTCACCGTTAACGGTGAGAAACAGTTTTACCACTGCTTTGGATGTGGCGCGCACGGCAACGCGATCGACTTCCTGATGAACTACGACAAGCTCGAGTTCGTCGAAACGGTCGAAGAGCTGGCAGCAATGCACaa                                                                                                                    >  2:876613/1‑150 (MQ=255)
                                   cGAGAAAACCCCGTCCTTCACCGTTAACGGTGAGAAACAGTTTTACCACTGCTTTGGATGTGGCGCGCACGGCAACGCGATCGACTTCCTGATGAACTACGACAAGCTCGAGTTCGTCGAAACGGTCGAAGAGCTGGCAGCAATGCACaa                                                                                                                    <  1:1228379/150‑1 (MQ=255)
                                    gagaAAACCCCGTCCTTCACCGTTAACGGTGAGAAACAGTTTTACCACTGCTTTGGATGTGGCGCGCACGGCAACGCGATCGACTTTCTGATGAACTACGACAAGCTCGAGTTCGTCGAAACGGTCGAAGAGCTGGCAGCAATGCACAAt                                                                                                                   >  2:3128945/1‑150 (MQ=255)
                                    gagaAAACCCCGTCCTTCACCGTTAACGGTGAGAAACAGTTTTACCACTGCTTTGGATGTGGCGCGCACGGCAACGCGATCGACTTCCTGATGAACTACGACAAGCTCGAGTTCGTCGAAACGGTCGAAGAGCTGGCAGCAATGCACAAt                                                                                                                   >  1:4402144/1‑150 (MQ=255)
                                     agaAAACCCCGTCCTTCACCGTTAACGGTGAGAAACAGTTTTACCACTGCTTTGGATGTGGCGCGCACGGCAACGCGATCGACTTTCTGATGAACTACGACAAGCTCGAGTTCGTCGAAACGGTCGAAGAGCTGGCAGCAATGCACAATc                                                                                                                  >  1:3924567/1‑150 (MQ=255)
                                     agaAAACCCCGTCCTTCACCGTTAACGGTGAGAAACAGTTTTACCACTGCTTTGGATGTGGCGCGCACGGCAACGCGATCGACTTCCTGATGAACTACGACAAGCTCGAGTTCGTCGAAACGGTCGAAGAGCTGGCAGCAATGCACAATc                                                                                                                  >  1:382963/1‑150 (MQ=255)
                                        aaaCCCCGTCCTTCACCGTTAACGGTGAGAAACAGTTTTACCACTGCTTTGGATGTGGCGCGCACGGCAACGCGATCGACTTCCTGATGAACTACGACAAGCTCGAGTTCGTCGAAACGGTCGAAGAGCTGGCAGCAATGCACAATCTTg                                                                                                               <  1:4321237/150‑1 (MQ=255)
                                            cccGTCCTTCACCGTTAACGGTGAGAAACAGTTTTACCACTGCTTTGGATGTGGCGCGCACGGCAACGCGATCGACTTCCTGATGAACTACGACAAGCTCGAGTTCGTCGAAACGGTCGAAGAGCTGGCAGCAATGCACAATCTTGAAGt                                                                                                           <  2:1319373/150‑1 (MQ=255)
                                              cGTCCTTCACCGTTAACGGTGAGAAACAGTTTTACCACTGCTTTGGATGTGGCGCGCACGGCAACGCGATCGACTTCCTGATGAACTACGACAAGCTCGAGTTCGTCGAAACGGTCGAAGAGCTGGCAGCAATGCACAATCTTGAAGTGc                                                                                                         >  1:1965781/1‑150 (MQ=255)
                                                     cACCGTTAACGGTGAGAAACAGTTTTACCACTGCTTTGGATGTGGCGCGCACGGCAACGCGATCGACTTCCTGATGAACTACGACAAGCTCGAGTTCGTCGAAACGGTCGAAGAGCTGGCAGCAATGCACAATCTTGAAGTGCCATTTGa                                                                                                  >  2:2092922/1‑150 (MQ=255)
                                                      aCCGTTAACGGTGAGAAACAGTTTTACCACTGCTTTGGATGTGGCGCGCACGGCAACGCGATCGACTTCCTGATGAACTACGACAAGCTCGAGTTCGTCGAAACGGTCGAAGAGCTGGCAGCAATGCACAATCTTGAAGTGCCATTTGaa                                                                                                 >  1:3101134/1‑150 (MQ=255)
                                                       ccGTTAACGGTGAGACACAGTTTTACCACTGCTTTGGATGTGGCGCGCACGGCAACGCGATCGACTTCCTGATGAACTACGACAAGCTCGAGTTCGTCGAAACGGTCGAAGAGCTGGCAGCAATGCACAATCTTGAAGTGCCATTTGAAg                                                                                                <  2:268885/150‑1 (MQ=255)
                                                         gTTAACGGTGAGAAACAGTTTTACCACTGCTTTGGATGTGGCGCGCACGGCAACGCGATCGACTTCCTGATGAACTACGACAAGCTCGAGTTCGTCGAAACGGTCGAAGAGCTGGCAGCAATGCACAATCTTGAAGTGCCATTTGAAgca                                                                                              <  1:1245877/150‑1 (MQ=255)
                                                            aaCGGTGAGAAACAGTTTTACCACTGCTTTGGATGTGGCGCGCACGGCAACGCGATCGACTTTCTGATGAACTACGACAAGCTCGAGTTCGTCGAAACGGTCGAAGAGCTGGCAGCAATGCACAATCTTGAAGTGCCATTTGAAgcaggc                                                                                           <  1:343708/150‑1 (MQ=255)
                                                                 tGAGAAACAGTTTTACCACTGCTTTGGATGTGGCGCGCACGGCAACGCGATCGACTTCCTGATGAACTACGACAAGCTCGAGTTCGTCGAAACGGTCGAAGAGCTGGCAGCAATGCACAATCTTGAAGTGCCATTTGAAGCAGGCAGCgg                                                                                      <  2:382869/150‑1 (MQ=255)
                                                                   agaAACAGTTTTACCACTGCTTTGGATGTGGCGCGCACGGCAACGCGATCGACTTCCTGATGAACTACGACAAGCTCGAGTTCGTCGAAACGGTCGAAGAGCTGGCAGCAATGCACAATCTTGAAGTGCCATTTGAAGCAGGCAGCGGcc                                                                                    >  2:1124199/1‑150 (MQ=255)
                                                                    gaAACAGTTTTACCACTGCTTTGGATGTGGCGCGCACGGCAACGCGATCGACTTCCTGATGAACTACGACAAGCTCGAGTTCGTCGAAACGGTCGAAGAGCTGGCAGCAATGCACAATCTTGAAGTGCCATTTGAAGCAGGCAGCGGccc                                                                                   >  1:1681731/1‑150 (MQ=255)
                                                                     aaaCAGTTTTACCACTGCTTTGGATGTGGCGCGCACGGCAACGCGATCGACTTTCTGATGAACTACGACAAGCTCGAGTTCGTCGAAACGGTCGAAGAGCTGGCAGCAATGCACAATCTTGAAGTGCCATTTGAAGCAGGCAGCGGcccc                                                                                  <  2:2813545/150‑1 (MQ=255)
                                                                      aaCAGTTTTACCACTGCTTTGGATGTGGCGCGCACGGCAACGCGATCGACTTCCTGATGAACTACGACAAGCTCGAGTTCGTCGAAACGGTCGAAGAGCTGGCAGCAATGCACAATCTTGAAGTGCCATTTGAAGCAGGCAGCGGCCcca                                                                                 >  2:1795530/1‑150 (MQ=255)
                                                                      aaCAGTTTTACCACTGCTTGGGATGTGGCGCGCACGGCAACGCGATCGACTTTCTGATGAACTACGACAAGCTCGAGTTCGTCGAAACGGTCGAAGAGCTGGCAGCAATGCACAATCTTGAAGTGCCATTTGAAGCAGGCAGCGGCCcca                                                                                 <  2:2596324/150‑1 (MQ=255)
                                                                       aCAGTTTTACCACTGCTTTGGATGTGGCGCGCACGGCACCGCGATCGACTTCCTGATGAACTACGACAAGCTCGAGTTCGTCGAAACGGTCGAAGAGCTGGCAGCAATGCACAATCTTGAAGTGCCATTTGAAGCAGGCAGCGGCCccag                                                                                <  1:3763259/150‑1 (MQ=255)
                                                                       aCAGTTTTACCACTGCTTTGGATGTGGCGCGCACGGCAACGCGATCGACTTCCTGATGAACTACGACAAGCTCGAGTTCGTCGAAACGGTCGAAGAGCTGGCAGCAATGCACAATCTTGAAGTGCCATTTGAAGCAGGCAGCGGCCccag                                                                                >  1:3781132/1‑150 (MQ=255)
                                                                               aCCACTGCTTTGGATGTGGCGCGCACGGCAACGCGATCGACTTCCTGATGAACTACGACAAGCTCGAGTTCGTCGAAACGGTCGAAGAGCTGGCAGCAATGCACAATCTTGAAGTGCCATTTGAAGCAGGCAGCGGCCCCAGCCAGATCg                                                                        >  1:518916/1‑150 (MQ=255)
                                                                                ccACTGCTTTGGATGTGGCGCGCACGGCAACGCGATCGACTTCCTGATGAACTACGACAAGCTCGAGTTCGTCGAAACGGTCGAAGAGCTGGCAGCAATGCACAATCTTGAAGTGCCATTTGAAGCAGGCAGCGGCCCCAGCCAGATCGa                                                                       >  1:1729642/1‑150 (MQ=255)
                                                                                  aCTGCTTTGGATGTGGCGCGCACGGCAACGCGATCGACTTCCTGATGAACTACGACAAGCTCGAGTTCGTCGAAACGGTCGAAGAGCTGGCAGCAATGCACAATCTTGAAGTGCCATTTGAAGCAGGCAGCGGCCCCAGCCAGATCGAgc                                                                     >  2:1180729/1‑150 (MQ=255)
                                                                                    tGCTTTGGATGTGGCGCGCACGGCAACGCGATCGACTTTCTGATGAACTACGACAAGCTCGAGTTCGTCGAAACGGTCGAAGAGCTGGCAGCAATGCACAATCTTGAAGTGCCATTTGAAGCAGGCAGCGGCCCCAGCCAGATCGAgcgc                                                                   >  2:2924194/1‑150 (MQ=255)
                                                                                       tttGGATGTGGCGCGCACGGCAACGCGATCGACTTCCTGATGAACTACGACAAGCTCGAGTTCGTCGAAACGGTCGAAGAGCTGGCAGCAATGCACAATCTTGAAGTGCCATTTGAAGCAGGCAGCGGCCCCAGCCAGATCGAGCGCCAt                                                                <  2:2237531/150‑1 (MQ=255)
                                                                                       tttGGATGTGGCGCGCACGGCAACGCGATCGACTTCCTGATGAACTACGACAAGCTCGAGTTCGTCGAAACGGTCGAAGAGCTGGCAGCAATGCACAATCTTGAAGTGCCATTTGAAGCAGGCAGCGGCCCCAGCCAGATCGAGCGCCAt                                                                <  2:2005343/150‑1 (MQ=255)
                                                                                        ttGGATGTGGCGCGCACGGCAACGCGATCGACTTTCTGATGAACTACGACAAGCTCGAGTTCGTCGAAACGGTCGAAGAGCTGGCAGCAATGCACAATCTTGAAGTGCCATTTGAAGCAGGCAGCGGCCCCAGCCAGATCGAGCGCCATc                                                               <  1:2221505/150‑1 (MQ=255)
                                                                                             tgtgGCGCGCACGGCAACGCGATCGACTTCCTGATGAACTACGACAAGCTCGAGTTCGTCGAAACGGTCGAAGAGCTGGCAGCAATGCACAATCTTGAAGTGCCATTTGAAGCAGGCAGCGGCCCCAGCCAGATCGAGCGCCATCAGAgg                                                          <  1:2531794/150‑1 (MQ=255)
                                                                                              gtgGCGCGCACGGCAACGCGATCGACTTCCTGATGAACTACGACACGCTCGAGTTCGTCGAAACGGTCGAAGAGCTGGCAGCAATGCACAATCTTGAAGTGCCATTTGAAGCAGGCAGCGGCCCCAGCCAGATCGAGCGCCATCAGAGGc                                                         <  1:971165/150‑1 (MQ=255)
                                                                                                  cgcgcACGGCAACGCGATCGACTTCCTGATGAACTACGACAAGCTCGAGTTCGTCGAAACGGTCGAAGAGCTGGCAGCAATGCACAATCTTGAAGTGCCATTTGAAGCAGGCAGCGGCCCCAGCCAGATCGAGCGCCATCAGAGGCAAAc                                                     <  2:1403698/150‑1 (MQ=255)
                                                                                                   gcgcACGGCAACGCGATCGACTTCCTGATGAACTACGACAAGCTCGAGTTCGTCGAAACGGTCGAAGAGCTGGCAGCAATGCACAATCTTGAAGTGCCATTTGAAGCAGGCAGCGGCCCCAGCCAGATCGAGCGCCATCAGAGGCAAACg                                                    <  2:1442334/150‑1 (MQ=255)
                                                                                                    cgcACGGCAACGCGATCGACTTCCTGATGAACTACGACAAGCTCGAGTTCGTCGAAACGGTCGAAGAGCTGGCAGCAATGCACAATCTTGAAGTGCCATTTGAAGCAGGCAGCGGCCCCAGCCAGATCGAGCGCCATCAGAGGCAAACGc                                                   <  2:4402144/150‑1 (MQ=255)
                                                                                                     gcACGGCAACGCGATTGACTTCCTGATGAACTACGACAAGCTCGAGTTAGTCGAAACGGTCGAAGAGCTGGCAGCAATGCACAATCTTGAAGTGCCATTTGAAGCAGGCAGCGGCCCCAGCCAGATCGAGCGCCATCAGAGGCAAACGCt                                                  <  1:3709217/150‑1 (MQ=255)
                                                                                                     gcACGGCAACGCGATTGACTTCCTGATGAACTACGACAAGCTCGAGTTAGTCGAAACGGTCGAAGAGCTGGCAGCAATGCACAATCTTGAAGTGCCATTTGAAGCAGGCAGCGGCCCCAGCCAGATCGAGCGCCATCAGAGGCAAACGCt                                                  <  1:1427214/150‑1 (MQ=255)
                                                                                                     gcACGGCAACGCGATCGACTTCCTGATGAACTACGACCAGCTCGAGTTCGTCGAACCGGTCGAAGAGCTGGCAGCAATGCACAATCTTGAAGTGCCATTTGAAGCAGGCAGCGGCCCCAGCCAGATCGAGCGCCATCAGAGGCAAACGCt                                                  <  1:310932/150‑1 (MQ=255)
                                                                                                     gcACGGCAACGCGATCGACTTCCTGATGAACTACGACAAGCTCGAGTTCGTCGAAACGGTCGAAGAGCTGGCAGCAATGCACAATCTTGAAGTGCCATTTGAAGCAGGCAGCGGCCCCAGCCAGATCGAGCGCCATCAGAGGCAATCGCt                                                  >  2:3467288/1‑150 (MQ=255)
                                                                                                     gcACGGCAACGCGATCGACTTCCTGATGAACTACGACAAGCTCGAGTTCGTCGAAACGGTCGAAGAGCTGGCAGCAATGCACAATCTTGAAGTGCCATTTGAAGCAGGCAGCGGCCCCAGCCAGATCGAGCGCCATCAGAGGCAAACGCt                                                  >  1:864637/1‑150 (MQ=255)
                                                                                                      cACGGCAACGCGATCGACTTCCTGATGAACTACGACAAGCTCGAGTTCGTCGAAACGGTCGAAGAGCTGGCAGCAATGCACAATCTTGAAGTGCCATTTGAAGCAGGCAGCGGCCCCAGCCAGATCGAGCGCCATCAGAGGCAAACGCtt                                                 <  1:1137720/150‑1 (MQ=255)
                                                                                                        cGGCAACGCGATCGACTTCCTGATGAACTACGACAAGCTCGAGTTCGTCGAAACGGTCGAAGAGCTGGCAGCAATGCACAATCTTGAAGTGCCATTTGAAGCAGGCAGCGGCCCCAGCCAGATCGAGCGCCATCAGAGGCAAACGCTTTa                                               >  1:2084036/1‑150 (MQ=255)
                                                                                                           cAACGCGATCGACTTCCTGATGAACTACGACAAGCTCGAGTTAGTCGAAACGGTCGAAGAGCTGGCAGCAATGCACAATCTTGAAGTGCCATTTGAAGCAGGCAGCGGCCCCAGCCAGATCGAGCGCCATCAGAGGCAAACGCTTTATCa                                            <  1:3262600/150‑1 (MQ=255)
                                                                                                               gcgATCGACTTCCTGATGAACTACGACAAGCTCGAGTTCGTCGAAACGGTCGAAGAGCTGGCAGCAATGCACAATCTTGAAGTGCCATTTGAAGCAGGCAGCGGCCCCAGCCAGATCGAGCGCCATCAGAGGCAAACGCTTTATCAGTTg                                        <  2:144673/150‑1 (MQ=255)
                                                                                                                cgATCGACTTCCTGATGAACTACGACAAGCTCGAGTTCGTCGAAACGGTCGAAGAGCTGGCAGCAATGCACAATCTTGAAGTGCCATTTGAAGCAGGCAGCGGCCCCAGCCAGATCGAGCGCCATCAGAGGCAAACGCTTTATCAGTTGa                                       >  2:3541492/1‑150 (MQ=255)
                                                                                                                cgATCGACTTCCTGATGAACTACGACAAGCTCGAGTTCGTCGAAACGGTCGAAGAGCTGGCAGCAATGCACAATCTTGAAGTGCCATTTGAAGCAGGCAGCGGCCCCAGCCAGATCGAGCGCCATCAGAGGCAAACGCTTTATCAGTTGa                                       >  1:4031322/1‑150 (MQ=255)
                                                                                                                 gATCGACTTTATGATGAACTACGACAAGCTCGAGTTCGTCGAAACGGTCGAAGAGCTGGCAGCAATGCACAATCTTGAAGTGCCATTTGAAGCAGGCAGCGGCCCCAGCCAGATCGAGCGCCATCAGAGGCAAACGCTTTATCAGTTGAt                                      <  1:4377433/150‑1 (MQ=255)
                                                                                                                 gATCGACTTCCTGATGAACTACGACAAGCTCGAGTTCGTCGAAACGGTCGAAGAGCTGGCAGCAATGCACAATCTTGAAGTGCCATTTGAAGCAGGCAGCGGCCCCAGCCAGATCGAGCGCCATCAGAGGCAAACGCTTTATCAGTTGAt                                      >  1:4241838/1‑150 (MQ=255)
                                                                                                                  aTCGACTTCCTGATGAACTCCGACAAGCTCGAGTTCGTCGAAACGGTCGAAGAGCTGGCAGCAATGCACAATCTTGAAGTGCCATTTGAAGCAGGCAGCGGCCCCAGCCAGATCGAGCGCCATCAGAGGCAAACGCTTTATCAGTTGATg                                     <  1:1799571/150‑1 (MQ=255)
                                                                                                                   tCGACTTCCTGATGAACTACGACAAGCTCGAGTTCGTCGAAACGGTCGAAGAGCTGGCAGCAATGCACAATCTTGAAGTGCCATTTGAAGCAGGCAGCGGCCCCAGCCAGATCGAGCGCCATCAGAGGCAAACGCTTTATCAGTTGATgg                                    <  2:1689433/150‑1 (MQ=255)
                                                                                                                    cGACTTTCTGATGAACTACGACAAGCTCGAGTTCGTCGAAACGGTCGAAGAGCTGGCAGCAATGCACAATCTTGAAGTGCCATTTGAAGCAGGCAGCGGCCCCAGCCAGATCGAGCGCCATCAGAGGCAAACGCTTTATCAGTTGATGGa                                   >  1:215073/1‑150 (MQ=255)
                                                                                                                    cGACTTCATGATGAACTACGTCAAGCTGGATTTCGTCGTAACGGTCGTAGAGCTTGCAGATATGCACAATCTTGATTTGCAATTTGAAGCAGGCAGCGGCCCCATCCAGATCGAGCGCCATCAGAGGCAAACGCTTTATCAGTTGATGGa                                   <  1:441199/150‑1 (MQ=255)
                                                                                                                       cTTTCTGATGAACTACGACAAGCTCGAGTTCGTCGAAACGGTCGAAGAGCTGGCAGCAATGCACAATCTTGAAGTGCCATTTGAAGCAGGCAGCGGCCCCAGCCAGATCGAGCGCCATCAGAGGCAAACGCTTTATCAGTTGATGGACgg                                >  2:1551111/1‑150 (MQ=255)
                                                                                                                          ccTGATGAACTACGACAAGCTCGAGTTCGTCGAAACGGTCGAAGAGCTGGCAGCAATGCACAATCTTGAAGTGCCATTTGAAGCAGGCAGCGGCCCCAGCCAGATCGAGCGCCATCAGAGGCAAACGCTTTATCAGTTGATGGACGGTCt                             >  1:2692243/1‑150 (MQ=255)
                                                                                                                            tgatgaACTCCGCCAAGCTCGAGTTAGTCGAAACGGTCGAAGAGCTGGCAGCAATGCACACTCTTGAAGTGCCATTTGAAGCAGGCAGCGGCCCCAGCCAGATCGAGCGCCATCAGAGGCAAACGCTTTATCAGTTGATGGACGGTCTGa                           <  1:2942596/150‑1 (MQ=255)
                                                                                                                               tgaACTACGACAAGCTCGAGTTCGTCGAAACGGTCGAAGAGCTGGCAGCAATGCACAATCTTGAAGTGCCATTTGAAGCAGGCAGCGGCCCCAGCCAGATCGAGCGCCATCAGAGGCAAACGCTTTATCAGTTGATGGACGGTCTGAATa                        >  1:268463/1‑150 (MQ=255)
                                                                                                                                gaACTACGACAAGCTCGAGTTCGTCGAAACGGTCGAAGAGCTGGCAGCAATGCACAATCTTGAAGTGCCATTTGAAGCAGGCAGCGGCCCCAGCCAGATCGAGCGCCATCAGAGGCAAACGCTTTATCAGTTGATGGACGGTCTGAATAc                       <  2:1118542/150‑1 (MQ=255)
                                                                                                                                  aCTACGACAAGCTCGAGTTCGTCGAAACGGTCGAAGAGCTGGCAGCAATGCACAATCTTGAAGTGCCATTTGAAGCAGGCAGCGGCCCCAGCCAGATCGAGCGCCATCAGAGGCAAACGCTTTATCAGTTGATGGACGGTCTGAATACGt                     <  1:1896400/150‑1 (MQ=255)
                                                                                                                                    tACGACAAGCTCGAGTTCGTCGAAACGGTCGAAGAGCTGGCAGCAATGCACAATCTTGAAGTGCCATTTGAAGCAGGCAGCGGCCCCAGCCAGATCGAGCGCCATCAGAGGCAAACGCTTTATCAGTTGATGGACGGTCTGAATACGttt                   >  2:3729567/1‑150 (MQ=255)
                                                                                                                                    tACGACAAGCTCGAGTTCGTCGAAACGGTCGAAGAGCTGGCAGCAATGCACAATCTTGAAGTGCCATTTGAAGCAGGCAGCGGCCCCAGCCAGATCGAGCGCCATCAGAGGCAAACGCTTTATCAGTTGATGGACGGTCTGAATACGttt                   <  2:885252/150‑1 (MQ=255)
                                                                                                                                      cGACAAGCTCGAGTTAGTCGAAACGGTCGAAGAGCTGGCAGCAATGCACAGTCTTGAAGTGCCATTAGAAGCAGGCAGCGGCCCCAGCCAGATCGAGCGCCATCAGAGGCAAACGCTTTATCAGTTGATGGACGGTCTGAATACGTTTTa                 >  1:4488175/1‑150 (MQ=255)
                                                                                                                                        aCAAGCTCGAGTTCGTCGAAACGGTCGAAGAGCTGGCAGCAATGCACAATCTTGAAGTGCCATTTGAAGCAGGCAGCGGCCCCAGCCAGATCGAGCGCCATCAGAGGCAAACGCTTTATCAGTTGATGGACGGTCTGAATACGTTTTAcc               <  1:605529/150‑1 (MQ=255)
                                                                                                                                        aCAAGCTCGAGTTCGTCGAAACGGTCGAAGAGCTGGCAGCAATGCACAATCTTGAAGTGCCATTTGAAGCAGGCAGCGGCCCCAGCCAGATCGAGCGCCATCAGAGGCAAACGCTTTATCAGTTGATGGACGGTCTGAATACGTTTTAcc               >  1:3051271/1‑150 (MQ=255)
                                                                                                                                         cAAGCTCGAGTTCGTCGAAACGGTCGAAGAGCTGGCAGCAATGCACAATCTTGAAGTGCCATTTGAAGCAGGCAGCGGCCCCAGCCAGATCGAGCGCCATCAGAGGCAAACGCTTTATCAGTTGATGGACGGTCTGAATACGTTTTACca              <  2:814437/150‑1 (MQ=255)
                                                                                                                                          aaGCTCGAGTTCGTCGAAACGGTCGAAGAGCTGGCAGCAATGCACAATCTTGAAGTGCCATTTGAAGCAGGCAGCGGCCCCAGCCAGATCGAGCGCCATCAGAGGCAAACGCTTTATCAGTTGATGGACGGTCTGAATACGTTTTACcaa             <  2:489141/150‑1 (MQ=255)
                                                                                                                                           aGCTCGAGTTCGTCGAAACGGTCGAAGAGCTGGCAGCAATGCACAATCTTGAAGTGCCATTTGAAGCAGGCAGCGGCCCCAGCCAGATCGAGCGCCATCAGAGGCAAACGCTTTATCAGTTGATGGACGGTCTGAATACGTTTTACcaac            <  2:3370990/150‑1 (MQ=255)
                                                                                                                                            gCTCGAGTTCGTCGAAACGGTCGAAGAGCTGGCAGCAATGCACAATCTTGAAGTGCCATTTGAAGCAGGCAGCGGCCCCAGCCAGATCGAGCGCCATCAGAGGCAAACGCTTTATCAGTTGATGGACGGTCTGAATACGTTTTACcaaca           >  1:802423/1‑150 (MQ=255)
                                                                                                                                               cGAGTTCGTCGAAACGGTCGAAGAGCTGGCAGCAATGCACAATCTTGAAGTGCCATTTGAAGCAGGCAGCGGCCCCAGCCAGATCGAGCGCCATCAGAGGCAAACGCTTTATCAGTTGATGGACGGTCTGAATACGTTTTACCAACAATc        <  1:3215501/150‑1 (MQ=255)
                                                                                                                                               cGAGTTCGTCGAAACGGTCGAAGAGCTGGCAGCAATGCACAATCTTGAAGTGCCATTTGAAGCAGGCAGCGGCCCCAGCCAGATCGAGCGCCATCAGAGGCAAACGCTTTATCAGTTGATGGACGGTCTGAATACGTTTTACCAACAATc        <  2:917498/150‑1 (MQ=255)
                                                                                                                                               cGAGTTCGTCGAAACGGTCGAAGAGCTGGCAGCAATGCACAATCTTGAAGTGCCATTTGAAGCAGGCAGCGGCCCCAGCCAGATCGAGCGCCATCAGAGACAAACGCTTTATCAGTTGATGGACGGTCTGAATACGTTTTACCAACAATc        <  1:3917411/150‑1 (MQ=255)
                                                                                                                                                gAGTTCGTCGAAACGGTCGAAGAGCTGGCAGCAATGCACAATCTTGAAGTGCCATTTGAAGCAGGCAGCGGCCCCAGCCAGATCGAGCGCCATCAGAGGCAAACGCTTTATCAGTTGATGGACGGTCTGAATACGTTTTACCAACAATCt       <  2:1965781/150‑1 (MQ=255)
                                                                                                                                                 aGTTCGTCGAAACGGTCGAAGAGCTGGCAGCAATGCACAATCTTGAAGTGCCATTTGAAGCAGGCAGCGGCCCCAGCCAGATCGAGCGCCATCAGAGGCAAACGCTTTATCAGTTGATGGACGGTCTGAATACGTTTTACCAACAATCtt      >  1:3592455/1‑150 (MQ=255)
                                                                                                                                                  gTTCGTCGAAACGGTCGAAGAGCTGGCAGCAATGCACAATCTTGAAGTGCCATTTGAAGCAGGCAGCGGCCCCAGCCAGATCGAGCGCCATCAGAGGCAAACGCTTTATCAGTTGATGGACGGTCTGAATACGTTTTACCAACAATCttt     <  1:3497183/150‑1 (MQ=255)
                                                                                                                                                  gTTCGTCGAAACGGTCGAAGAGCTGGCAGCAATGCACAATCTTGAAGTGCCATTTGAAGCAGGCAGCGGCCCCAGCCAGATCGAGCGCCATCAGAGGCAAACGCTTTATCAGTTGATGGACGGTCTGAATACGTTTTACCAACAATCttt     <  1:3123143/150‑1 (MQ=255)
                                                                                                                                                  gTTCGTCGAAACGGTCGAAGAGCTGGCAGCAATGCACAATCTTGAAGTGCCATTTGAAGCAGGCAGCGGCCCCAGCCAGATCGAGCGCCATCAGAGGCAAACGCTTTATCAGTTGATGGACGGTCTGAATACGTTTTACCAACAATCttt     <  1:1832299/150‑1 (MQ=255)
                                                                                                                                                  gTTCGTCGAAACGGTCGAAGAGCTGGCAGCAATGCACAATCTTGAAGTGCCATTTGAAGCAGGCAGCGGCCCCAGCCAGATCGAGCGCCATCAGAGGCAAACGCTTTATCAGTTGATGGACGGTCTGAATACGTTTTACCAACAATCtat     >  1:4270232/1‑148 (MQ=255)
                                                                                                                                                   ttCGTCGAAACGGTCGAAGAGCTGGCAGCAATGCACAATCTTGAAGTGCCATTTGAAGCAGGCAGCGGCCCCAGCCAGATCGAGCGCCATCAGAGGCAAACGCTTTATCAGTTGATGGACGGTCTGAATACGTTTTACCAACAATCTTTa    <  1:1016204/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
GGCAAGAATTTCCACGCGTGTTGTCCATTCCACAACGAGAAAACCCCGTCCTTCACCGTTAACGGTGAGAAACAGTTTTACCACTGCTTTGGATGTGGCGCGCACGGCAACGCGATCGACTTCCTGATGAACTACGACAAGCTCGAGTTCGTCGAAACGGTCGAAGAGCTGGCAGCAATGCACAATCTTGAAGTGCCATTTGAAGCAGGCAGCGGCCCCAGCCAGATCGAGCGCCATCAGAGGCAAACGCTTTATCAGTTGATGGACGGTCTGAATACGTTTTACCAACAATCTTTACA  >  CP009273/3204562‑3204860

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑