Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,204,985 G→T 5.3% G174C (GGC→TGC)  dnaG → DNA primase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,204,9850GT 5.3% 942.1 / 22.8 244G174C (GGC→TGC) dnaGDNA primase
Reads supporting (aligned to +/- strand):  ref base G (101/130);  new base T (8/5);  total (109/135)
Fisher's exact test for biased strand distribution p-value = 2.57e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.71e-01

Only 100 of 253 total aligned reads displayed.

ATACGTTTTACCAACAATCTTTACAACAACCTGTTGCCACGTCTGCGCGCCAGTATCTGGAAAAACGCGGATTAAGCCACGAGGTTATCGCTCGCTTTGCGATTGGTTTTGCGCCCCCCGGCTGGGACAACGTCCTGAAGCGGTTTGGCGGCAATCCAGAAAATCGCCAGTCATTGATTGATGCGGGGATGTTGGTCACTAACGATCAGGGACGCAGTTACGATCGTTTCCGCGAGCGGGTGATGTTCCCCATTCGCGATAAACGCGGTCGGGTGATTGGTTTTGGCGGGCGCGTGCTG  >  CP009273/3204836‑3205134
                                                                                                                                                     |                                                                                                                                                     
aTACGTTTTACCAACAATCTTTACAACAACCTGTTGCCACGTCTGCGCGCCAGTATCTGGAAAAACGCGGATTAAGCCACGAGGTTATCGCTCGCTTTGCGATTGGTTTTGCGCCCCCCGGCTGGGACAACGTCCTGAAGCGGTTTggcg                                                                                                                                                       <  2:3132581/150‑1 (MQ=255)
aTACGTTTTACCAACAATCTTTACAACAACCTGTTGCCACGTCTGCGCGCCAGTATCTGGAAAAACGCGGATTAAGCCACGAGGTTATCGCTCGCTTTGCGATTGGTTTTGCGCCCCCCGGCTGGGACAACGTCCTGAAGCGGTTTggcg                                                                                                                                                       <  2:864637/150‑1 (MQ=255)
 tACGTTTTACCAACAATCTTTACAACAACCTGTTGCCACGTCTGCGCGCCAGTATCTGGAAAAACGCGGATTAAGCCACGAGGTTATCGCTCGCTTTGCGATTGGTTTTGCGCCCCCCGGCTGGGACAACGTCCTGAAGCGGTTTggcgg                                                                                                                                                      >  2:3851714/1‑150 (MQ=255)
     ttttACCAACAATCTTTACAACAACCTGTTGCCACGTCTGCGCGCCAGTATCTGGAAAAACGCGGATTAAGCCACGAGGTTATCGCTCGCTTTGCGATTGGTTTTGCGCCCCCCGGCTGGGACAACGTCCTGAAGCGGTTTGGCGGCAAt                                                                                                                                                  <  2:1099226/150‑1 (MQ=255)
       ttACCAACAATCTTTACAACAACCTGTTGCCACGTCTGCGCGCCAGTATCTGGAAAAACGCGGATTAAGCCACGAGGTTATCGCTCGCTTTGCGATTGGTTTTGCGCCCCCCGGCTGGGACAACGTCCTGAAGCGGTTTGGCGGCAATcc                                                                                                                                                <  1:2309606/150‑1 (MQ=255)
        tACCAACAATCTTTACAACAACCTGTTGCCACGTCTGCGCGCCAGTATCTGGAAAAACGCGGATTAAGCCACGAGGTTATCGCTCGCTTTGCGATTGGTTTTGCGCCCCCCGGCTGGGACAACGTCCTGAAGCGGTTTGGCGGCACTCCa                                                                                                                                               >  1:4400418/1‑150 (MQ=255)
        tACCAACAATCTTTACAACAACCTGTTGCCACGTCTGCGCGCCAGTATCTGGAAAAACGCGGATTAAGCCACGAGGTTATCGCTCGCTTTGCGATTGGTTTTGCGCCCCCCGGCTGGGACAACGTCCTGAAGCGGTTTGGCGGCAATCCa                                                                                                                                               >  1:927363/1‑150 (MQ=255)
           caacaaTCTTTACAACAACCTGTTGCCACGTCTGCGCGCCAGTATCTGGAAAAACGCGGATTAAGCCACGAGGTTATCGCTCGCTTTGCGATTGGTTTTGCGCCCCCCGGCTGGGACAACGTCCTGAAGCGGTTTGGCGGCAATCCAGaa                                                                                                                                            >  2:1860726/1‑150 (MQ=255)
               actCTTTACAACAACCTGTTGCCACGTCTGCGCGCCAGTATCTGGAAAAACGCGGATTAAGCCACGAGGTTATCGCTCGCTTTGCGATTGGTTTTGCGCCCCCCGGCTGGGACAACGTCCTGAAGCGGTTTGGCGGCAATCCAGAAAATc                                                                                                                                        <  1:2498894/148‑1 (MQ=255)
               aaTCTTTACAACAACCTGTTGCCACGTCTGCGCGCCAGTATCTGGAAAAACGCGGATTAAGCCACGAGGTTATCGCTCGCTTTGCGATTGGTTTTGCGCCCCCCGGCTGGGACAACGTCCTGAAGCGGTTTGGCGGCAATCCAGAAAATc                                                                                                                                        >  2:1427725/1‑150 (MQ=255)
               aaTCTTTACAACAACCTGTTGCCACGTCTGCGCGCCAGTATCTGGAAAAACGCGGATTAAGCCACGAGGTTATCGCTCGCTTTGCGATTGGTTTTGCGCCCCCCGGCTGGGACAACGTCCTGAAGCGGTTTGGCGGCAATCCAGAAAATc                                                                                                                                        >  2:3719110/1‑150 (MQ=255)
                   tttACAACAACCTGTTGCCACGTCTGCGCGCCAGTATCTGGAAAAACGCGGATTAAGCCACGAGGTTATCGCTCGCTTTGCGATTGGTTTTGCGCCCCCCGGCTGGGACAACGTCCTGAAGCGGTTTGGCGGCAATCCAGAAAATCGCCa                                                                                                                                    >  2:4364770/1‑150 (MQ=255)
                    ttACAACAACCTGTTGCCACGTCTGCGCGCCAGTATCTGGAAAAACGCGGATTAAGCCACGAGGTTATCGCTCGCTTTGCGATTGGTTTTGCGCCCCCCGGCTGGGACAACGTCCTGAAGCGGTTTGGCGGCAATCCAGAAAATCGCCAg                                                                                                                                   <  2:3051271/150‑1 (MQ=255)
                    ttACAACAACCTGTTGCCACGTCTGCGCGCCAGTATCTGGAAAAACGCGGATTAAGCCACGAGGTTATCGCTCGCTTTGCGATTGGTTTTGCGCCCCCCGGCTGGGACAACGTCCTGAAGCGGTTTGGCGGCAATCCAGAAAATCGCCAg                                                                                                                                   <  1:876628/150‑1 (MQ=255)
                    ttACAACAACCTGTTGCCACGTCTGCGCGCCAGTATCTGGAAAAACGCGGATTAAGCCACGAGGTTATCGCTCGCTTTGCGATTGGTTTTGCGCCCCCCGGCTGGGACAACGTCCTGAAGCGGTTTGGCGGCAATCCAGAAAATCGCCAg                                                                                                                                   >  1:1046276/1‑150 (MQ=255)
                       caacaaCCTGTTGCCACGTCTGCGCGCCAGTATCTGGAAAAACGCGGATTAAGCCACGAGGTTATCGCTCGCTTTGCGATTGGTTTTGCGCCCCCCGGCTGGGACAACGTCCTGAAGCGGTTTGGCGGCAATCCAGAAAATCGCCAGTCa                                                                                                                                <  2:4311472/150‑1 (MQ=255)
                        aacaacCTGTTGCCACGTCTGCGCGCCAGTATCTGGAAAAACGCGGATTAAGCCACGAGGTTATCGCTCGCTTTGCGATTGGTTTTGCGCCCCCCGGCTGGGACAACGTCCTGAAGCGGTTTGGCGGCAATCCAGAAAATCGCCAGTCat                                                                                                                               <  2:3924597/150‑1 (MQ=255)
                        aacaacCTGTTGCCACGTCTGCGCGCCAGTATCTGGAAAAACGCGGATTAAGCCACGAGGTTATCGCTCGCTTTGCGATTGGTTTTGCGCCCCCCGGCTGGGACAACGTCCTGAAGCGGTTTGGCGGCAATCCAGAAAATCGCCAGTCat                                                                                                                               <  2:156380/150‑1 (MQ=255)
                        aacaacCTGTTGCCACGTCTGCGCGCCAGTATCTGGAAAAACGCGGATTAAGCCACGAGGTTATCGCTCGCTTTGCGATTGGTTTTGCGCCCCCCGGCTGGGACAACGTCCTGAAGCGGTTTGGCGGCAATCCAGAAAATCGCCAGTCat                                                                                                                               <  2:3924544/150‑1 (MQ=255)
                        aacaacCTGTTGCCACGTCTGCGCGCCAGTATCTGGAAAAACGCGGATTAAGCCACGAGGTTATCGCTCGCTTTGCGATTGGTTTTGCGCCCCCCGGCTGGGACAACGTCCTGAAGCGGTTTGGCGGCAATCCAGAAAATCGCCAGTCat                                                                                                                               >  1:523001/1‑150 (MQ=255)
                           aacCTGTTGCCACGTCTGCGCGCCAGTATCTGGAAAAACGCGGATTAAGCCACGAGGTTATCGCTCGCTTTGCGATTGGTTTTGCGCCCCCCGGCTGGGACAACGTCCTGAAGCGGTTTGGCTGCAATCCAGAAAATCGCCAGTCattga                                                                                                                            >  1:566943/1‑150 (MQ=255)
                              cTGTTGCCACGTCTGCGCGCCAGTATCTGGAAAAACGCGGATTAAGCCACGAGGTTATCGCTCGCTTTGCGATTGGTTTTGCGCCCCCCGGCTGGGACAACGTCCTGAAGCGGTTTGGCGGCAATCCAGAAAATCGCCAGTCattgattg                                                                                                                         >  2:3383270/1‑150 (MQ=255)
                                ggtGCCACGTCTGCGCGCCAGTATCTGGAAAAACGCGGATTAAGCCACGAGGTTATCGCTCGCTTTGCGATTGGTTTTGCGCCCCCCGGCTGGGACAACGTCCTGAAGCGGTTTGGCGGCAATCCAGAAAATCGCCAGTCATtgattgat                                                                                                                       <  2:548052/148‑1 (MQ=255)
                                   gCCACGTCTGCGCGCCAGTATCTGGAAAAACGCGGATTAAGCCACGAGGTTATCGCTCGCTTTGCGATTGGTTTTGCGCCCCCCGGCTGGGACAACGTCCTGAAGCGGTTTGGCGGCAATCCAGAAAATCGCCAGTCATTGATTGATGCg                                                                                                                    <  2:3818024/150‑1 (MQ=255)
                                        gTCTGCGCGCCAGTATCTGGAAAAACGCGGATTAAGCCACGAGGTTATCGCTCGCTTTGCGATTGGTTTTGCGCCCCCCGGCTGGGACAACGTCCTGAAGCGGTTTGGCGGCAATCCAGAAAATCGCCAGTCATTGATTGATGCGGGGAt                                                                                                               <  2:518916/150‑1 (MQ=255)
                                        gTCTGCGCGCCAGTATCTGGAAAAACGCGGATTAAGCCACGAGGTTATCGCTCGCTTTGCGATTGGTTTTGCGCCCCCCGGCTGGGACAACGTCCTGAAGCGGTTTGGCGGCAATCCAGAAAATCGCCAGTCATTGATTGATGCGGGGAt                                                                                                               <  2:715203/150‑1 (MQ=255)
                                            gcgcgcCAGTATCTGGAAAAACGCGGATTAAGCCACGAGGTTATCGCTCGCTTTGCGATTGGTTTTGCGCCCCCCGGCTGGGACAACGTCCTGAAGCGGTTTGGCGGCAATCCAGAAAATCGCCAGTCATTGATTGATGCGGGGATGTTg                                                                                                           <  2:4067233/150‑1 (MQ=255)
                                            gcgcgcCAGTATCTGGAAAAACGCGGATTAAGCCACGAGGTTATCGCTCGCTTTGCGATTGGTTTTGCGCCCCCCGGCTGGGACAACGTCCTGAAGCGGTTTGGCGGCAATCCAGAAAATCGCCAGTCATTGATTGATGCGGGGATGTTg                                                                                                           <  1:23461/150‑1 (MQ=255)
                                             cgcgcCAGTATCTGGAAAAACGCGGATTAAGCCACGAGGTTATCGCTCGCTTTGCGATTGGTTTTGCGCCCCCCGGCTGGGACAACGTCCTGAAGCGGTTTGGCGGCAATCCAGAAAATCGCCAGTCATTGATTGATGCGGGGATGTTgg                                                                                                          <  2:1181712/150‑1 (MQ=255)
                                               cgcCAGTATCTGGAAAAACGCGGATTAAGCCACGAGGTTATCGCTCGCTTTGCGATTGGTTTTGCGCCCCCCGGCTGGGACAACGTCCTGAAGCGGTTTGGCGGCAATCCAGAAAATCGCCAGTCATTGATTGATGCGGGGATGTTGGTc                                                                                                        <  2:889496/150‑1 (MQ=255)
                                                gcCAGTATCTGGAAAAACGCGGATTAAGCCACGAGGTTATCGCTCGCTTTGCGATTGGTTTTGCGCCCCCCGGCTGGGACAACGTCCTGAAGCGGTTTGGCGGCAATCCAGAAAATCGCCAGTCATTGATTGATGCGGGGATGTTGGTCa                                                                                                       >  1:3948743/1‑150 (MQ=255)
                                                 ccAGTATCTGGAAAAACGCGGATTAAGCCACGAGGTTATCGCTCGCTTTGCGATTGGTTTTGCGCCCCCCGGCTGGGACAACGTCCTGAAGCGGTTTGGCGGCAATCCAGAAAATCGCCAGTCATTGATTGATGCGGGGATGTTGGTCAc                                                                                                      <  2:4488158/150‑1 (MQ=255)
                                                  cAGTATCTGGAAAAACGCGGATTAAGCCACGAGGTTATCGCTCGCTTTGCGATTGGTTTTGCGCCCCCCGGCTGGGACAACGTCCTGAAGCGGTTTGGCGGCAATCCAGAAAATCGCCAGTCATTGATTGATGCGGGGATGTTGGTCACt                                                                                                     >  1:1545443/1‑150 (MQ=255)
                                                      aTCTGGAAAAACGCGGATTAAGCCACGAGGTTATCGCTCGCTTTGCGATTGGTTTTGCGCCCCCCGGCTGGGACAACGTCCTGAAGCGGTTTGGCGGCAATCCAGAAAATCGCCAGTCATTGATTGATGCGGGGATGTTGGTCACTAACg                                                                                                 <  2:268463/150‑1 (MQ=255)
                                                      aTCTGGAAAAACGCGGATTAAGCCACGAGGTTATCGCTCGCTTTGCGATTGGTTTTGCGCCCCCCGGCTGGGACAACGTCCTGAAGCGGTTTGGCGGCAATCCAGAAAATCGCCAGTCATTGATTGATGCGGGGATGTTGGTCACTAACg                                                                                                 <  2:1355992/150‑1 (MQ=255)
                                                        cTGGAAAAACGCGGATTAAGCCACGAGGTTATCGCTCGCTTTGCGATTGGTTTTGCGCCCCCCGGCTGGGACAACGTCCTGAAGCGGTTTGGCGGCAATCCAGAAAATCGCCAGTCATTGATTGATGCGGGGATGTTGGTCACTAACGAt                                                                                               <  2:2517562/150‑1 (MQ=255)
                                                         tGGAAAAACGCGGATTAAGCCACGAGGTTATCGCTCGCTTTGCGATTGGTTTTGCGCCCCCCGGCTGGGACAACGTCCTGAAGCGGTTTGGCGGCAATCCAGAAAATCGCCAGTCATTGATTGATGCGGGGATGTTGGTCACTAACGATc                                                                                              <  1:4380721/150‑1 (MQ=255)
                                                         tGGAAAAACGCGGATTAAGCCACGAGGTTATCGCTCGCTTTGCGATTGGTTTTGCGCCCCCCGGCTGGGACAACGTCCTGAAGCGGTTTGGCGGCAATCCAGAAAATCGCCAGTCATTGATTGATGCGGGGATGTTGGTCACTAACGATc                                                                                              <  1:3541492/150‑1 (MQ=255)
                                                          ggAAAAACGCGGATTAAGCCACGAGGTTATCGCTCGCTTTGCGATTGGTTTTGCGCCCCCCGGCTGGGACAACGTCCTGAAGCGGTTTGGCGGCAATCCAGAAAATCGCCAGTCATTGATTGATGCGGGGATGTTGGTCACTAACGATCa                                                                                             <  1:1180729/150‑1 (MQ=255)
                                                           gAAAAACGCGGATTAAGCCACGAGGTTATCGCTCGCTTTGCGATTGGTTTTGCGCCCCCCGGCTGGGACAACGTCCTGAAGCGGTTTGGCTGCAATCCAGAAAATCGCCAGTCATTGATTGATGCGGGGATGTTGGTCACTAACGATCAg                                                                                            <  2:2596981/150‑1 (MQ=255)
                                                           gAAAAACGCGGATAAAGCCACGAGGTTATCGCTCGCTTTGCGATTGGTTTTGCGCCCCCCGGCTGGGACAACGTCCTGAAGCGGTTTGGCTGCAATCCAGAAAATCGCCAGTCATTGATTGATGCGGGGATGTTGGTCACTAACGATCAg                                                                                            <  2:320423/150‑1 (MQ=255)
                                                               aaCGCGGATTAAGCCACGAGGTTATCGCTCGCTTTGCGATTGGTTTTGCGCCCCCCGGCTGGGACAACGTCCTGAAGCGGTTTGGCGGCAATCCAGAAAATCGCCAGTCATTGATTGATGCGGGGATGTTGGTCACTAACGATCAGGGAc                                                                                        <  1:3509765/150‑1 (MQ=255)
                                                                    ggATTAAGCCACGAGGTTATCGCTCGCTTTGCGATTGGTTTTGCGCCCCCCGGCTGGGACAACGTCCTGAAGCGGTTTGGCGGCAATCCAGAAAATCGCCAGTCATTGATTGATGCGGGGATGTTGGTCACTAACGATCAGGGACGCAGt                                                                                   <  2:371402/150‑1 (MQ=255)
                                                                       ttAAGCCACGAGGTTATCGCTCGCTTTGCGATTGGTTTTGCGCCCCCCGGCTGGGACAACGTCCTGAAGCGGTTTGGCGGCAATCCAGAAAATCGCCAGTCATTGATTGATGCGGGGATGTTGGTCACTAACGATCAGGGACGCAGTTAc                                                                                <  1:3220739/150‑1 (MQ=255)
                                                                       ttAAGCCACGAGGTTATCGCTCGCTTTGCGATTGGTTTTGCGCCCCCCGGCTGGGACAACGTCCTGAAGCGGTTTGGCGGCAATCCAGAAAATCGCCAGTCATTGATTGATGCGGGGATGTTGGTCACTAACGATCAGGGACGCAGTTAc                                                                                <  1:3716216/150‑1 (MQ=255)
                                                                        tAAGCCACGAGGTTATCGCTCGCTTTGCGATTGGTTTTGCGCCCCCCGGCTGGGACAACGTCCTGAAGCGGTTTGGCTGCAATCCAGAAAATCGCCAGTCATTGATTGATGCGGGGATGTTGGTCACTAACGATCAGGGACGCAGTTACg                                                                               >  1:3768148/1‑150 (MQ=255)
                                                                         aaGCCACGAGGTTATCGCTCGCTTTGCGATTGGTTTTGCGCCCCCCGGCTGGGACAACGTCCTGAAGCGGTTTGGCGGCAATCCAGAAACTCGCCAGTCATTGATTGATGCGGGGATGTTGGTCACTAACGATCAGGGACGCAGTTACGa                                                                              >  1:2833678/1‑150 (MQ=255)
                                                                         aaGCCACGAGGTTATCGCTCGCTTTGCGATTGGTTTTGCGCCCCCCGGCTGGGACAACGTCCTGAAGCGGTTTGGCGGCAATCCAGAAAATCGCCAGTCATTGATTGATGCGGGGATGTTGGTCACTAACGATCAGGGACGCAGTTACGa                                                                              >  1:2995542/1‑150 (MQ=255)
                                                                          aGCCACGAGGTTATCGCTCGCTTTGCGATTGGTTTTGCGCCCCCCGGCTGGGACAACGTCCTGAAGCGGTTTGGCGGCAATCCAGAAAATCGCCAGTCATTGATTGATGCGGGGATGTTGGTCACTAACGATCAGGGACGCAGTTACGAt                                                                             >  1:4351366/1‑150 (MQ=255)
                                                                          aGCCACGAGGTTATCGCTCGCTTTGCGATTGGTTTTGCGCCCCCCGGCTGGGACAACGTCCTGAAGCGGTTTGGCGGCAATCCAGAAAATCGCCAGTCATTGATTGATGCGGGGATGTTGGTCACTAACGATCAGGGACGCAGTTACGAt                                                                             <  2:2692243/150‑1 (MQ=255)
                                                                          aGCCACGAGGTTATCGCTCGCTTTGCGATTGGTTTTGCGCCCCCCGGCTGGGACAACGTCCTGAAGCGGTTTGGCGGCAATCCAGAAAATCGCCAGTCATTGATTGATGCGGGGATGTTGGTCACTAACGATCAGGGACGCAGTTACGAt                                                                             >  2:2448792/1‑150 (MQ=255)
                                                                           gCCACTAGGTTATCGCTCGCTTTGCGATTGGTTTTGCGCCCCCCGGCTGGGACAACGTCCTGAAGCGGTTTGGCGGCAATCCAGAAAATCGCCAGTCATTGATTGATGCGGGGATGTTGGTCACTAACGATCAGGGACGCAGTTACGATc                                                                            <  1:1390538/150‑1 (MQ=255)
                                                                              aCGAGGTTATCGCTCGCTTTGCGATTGGTTTTGCGCCCCCCGGCTGGGACAACGTCCTGAAGCGGTTTGGCGGCAATCCAGAAAATCGCCAGTCATTGATTGATGCGGGGATGTTGGTCACTAACGATCAGGGACGCAGTTACGATCGtt                                                                         <  1:1745164/150‑1 (MQ=255)
                                                                               cGAGGTTATCGCTCGCTTTGCGATTGGTTTTGCGCCCCCCGGCTGGGACAACGTCCTGAAGCGGTTTGGCGGCAATCCAGAAAATCGCCAGTCATTGATTGATGCGGGGATGTTGGTCACTAACGATCAGGGACGCAGTTACGATCGttt                                                                        <  2:1681731/150‑1 (MQ=255)
                                                                               cGAGGTTATCGCTCGCTTTGCGATTGGTTTTGCGCCCCCCGGCTGGGACAACGTCCTGAAGCGGTTTGGCGGCAATCCAGAAAATCGCCAGTCATTGATTGATGCGGGGATGTTGGTCACTAACGATCAGGGACGCAGTTACGATCGttt                                                                        >  1:635471/1‑150 (MQ=255)
                                                                               cGAGGTTATCGCTCGCTTTGCGATTGGTTTTGCGCCCCCCGGCTGGGACAACGTCCTGAAGCGGTTTGGCGGCAATCCAGAAAATCGCCAGTCATTGATTGATGCGGGGATGTTGGTCACTAACGATCAGGGACGCAGTTACGATCGttt                                                                        >  1:3721380/1‑150 (MQ=255)
                                                                               cGAGGTTATCGCTCGCTTTGCGATTGGTTTTGCGCCCCCCGGCTGGGACAACGTCCTGAAGCGGTTTGGCGGCAATCCAGAAAATCGCCAGTCATTGATTGATGCGGGGATGTTGGTCACTAACGATCAGGGACGCAGTTACGATCGttt                                                                        <  2:1229872/150‑1 (MQ=255)
                                                                               cGAGGTTATCGCTCGCTGTGCGATTGGTTTTGCGCCCCCCGGCTGGGACAACGTCCTGAAGCGGTTTGGCTGCAATCCAGAAAATCGCCAGTCATTGATTGATGCGGGGATGTTGGTCACTAACGATCAGGGACGCAGTTACGATCGttt                                                                        <  1:1650918/150‑1 (MQ=255)
                                                                                 aGGTTATCGCTCGCTTTGCGATTGGTTTTGCGCCCCCCGGCTGGGACAACGTCCTGAAGCGGTTTGGCGGCAATCCAGAAAATCGCCAGTCATTGATTGATGCGGGGATGTTGGTCACTAACGATCAGGGACGCAGTTACGATCGTTTcc                                                                      <  2:1934918/150‑1 (MQ=255)
                                                                                  ggTTATCGCTCGCTTTGCGATTGGTTTTGCGCCCCCCGGCTGGGACAACGTCCTGAAGCGGTTTGGCGGCAATCCAGAAAATCGCCAGTCATTGATTGATGCGGGGATGTTGGTCACTAACGATCAGGGACGCAGTTACGATCGTTTCcg                                                                     <  1:1866234/150‑1 (MQ=255)
                                                                                    ttATCGCTCGCTTTGCGATTGGTTTTGCGCCCCCCGGCTGGGACAACGTCCTGAAGCGGTTTGGCGGCAATCCAGAAAATCGCCAGTCATTGATTGATGCGGGGATGTTGGTCACTAACGATCAGGGACGCAGTTACGATCGTTTCcgcg                                                                   >  2:3128436/1‑150 (MQ=255)
                                                                                    ttATCGCTCGCTTTGCGATTGGTTTTGCGCCCCCCGGCTGGGACAACGTCCTGAAGCGGTTTGGCGGCAATCCAGAAAATCGCCAGTCATTGATTGATGCGGGGATGTTGGTCACTAACGATCAGGGACGCAGTTACGATCGTTTCcgcg                                                                   >  2:677782/1‑150 (MQ=255)
                                                                                     tATCGCTCGCTTTGCGATTGGTTTTGCGCCCCCCGGCTGGGACAACGTCCTGAAGCGGTTTGGCGGCAATCCAGAAAATCGCCAGTCATTGATTGATGCGGGGATGTTGGTCACTAACGATCAGGGACGCAGTTACGATCGTTTCCGCGa                                                                  >  2:201493/1‑150 (MQ=255)
                                                                                      aTCGCTCGCTTTGCGATTGGTTTTGCGCCCCCCGGCTGGGACAACGTCCTGAAGCGGTTTGGCGGCAATCCAGAAAATCGCCAGTCATTGATTGATGCGGGGATGTTGGTCACTAACGATCAGGGACGCAGTTACGATCGTTTCCGCGAg                                                                 >  2:3421098/1‑150 (MQ=255)
                                                                                      aTCGCTCGCTTTGCGATTGGTTTTGCGCCCCCCGGCTGGGACAACGTCCTGAAGCGGTTTGGCGGCAATCCAGAAAATCGCCAGTCATTGATTGATGCGGGGATGTTGGTCACTAACGATCAGGGACGCAGTTACGATCGTTTCCGCGAg                                                                 >  1:3169493/1‑150 (MQ=255)
                                                                                          ctcgctTTGCGATTGGTTTTGCGCCCCCCGGCTGGGACAACGTCCTGAAGCGGTTTGGCGGCAATCCAGAAAATCGCCAGTCATTGATTGATGCGGGGATGTTGGTCACTAACGATCAGGGACGCAGTTACGATCGTTTCCGCGAGCggg                                                             >  1:3765972/1‑150 (MQ=255)
                                                                                            cgctTTGCGATTGGTTTTGCGCCCCCCGGCTGGGACAACGTCCTGAAGCGGTTTGGCGGCAATCCAGAAAATCGCCAGTCATTGATTGATGCGGGGATGTTGGTCACTAACGATCAGGGACGCAGTTACGATCGTTTCCGCGAGCGGGTg                                                           <  2:1327238/150‑1 (MQ=255)
                                                                                              ctgtGCGATTGGTTTTGCGCCCCCCGGCTGGGACAACGTCCTGAAGCGGTTTGGCGGCAATCCAGAAAATCGCCAGTCATTGATTGATGCGGGGATGTTGGTCACTAACGATCAGGGACGCAGTTACGATCGTTTCCGCGAGCGGGTGAt                                                         <  2:1520084/147‑1 (MQ=255)
                                                                                                ttGCGATTGGTTTTGCGCCCCCCGGCTGGGACAACGTCCTGAAGCGGTTTGGCGGCAATCCAGAAAATCGCCAGTCATTGATTGATGCGGGGATGTTGGTCACTAACGATCAGGGACGCAGTTACGATCGTTTCTGCGAGCGGGTGATGt                                                       <  1:868557/150‑1 (MQ=255)
                                                                                                 tGCGATTGGTTTTGCGCCCCCCGGCTGGGACAACGTCCTGAAGCGGTTTGGCGGCAATCCAGAAAATCGCCAGTCATTGATTGATGCGGGGATGTTGGTCACTAACGATCAGGGACGCAGTTACGATCGTTTCCGCGAGCGGGTGATGtt                                                      <  2:2173154/150‑1 (MQ=255)
                                                                                                    gATTGGTTTTGCGCCCCCCGGCTGGGACAACGTCCTGAAGCGGTTTGGCGGCAATCCAGAAAATCGCCAGTCATTGATTGATGCGGGGATGTTGGTCACTAACGATCAGGGACGCAGTTACGATCGTTTCCGCGAGCGGGTGATGTTccc                                                   >  2:2365997/1‑150 (MQ=255)
                                                                                                          ttttGCGCCCCCCGGCTGGGACAACGTCCTGAAGCGGTTTGGCGGCAATCCAGAAAATCGCCAGTCATTGATTGATGCGGGGATGTTGGTCACTAACGATCAGGGACGCAGTTACGATCGTTTCCGCGAGCGGGTGATGTTCCCCATTcg                                             <  1:4107169/150‑1 (MQ=255)
                                                                                                            ttGCGCCCCCCGGCTGGGACAACGTCCTGAAGCGGTTTGGCGGCAATCCAGAAAATCGCCAGTCATTGATTGATGCGGGGATGTTGGTCACTAACGATCAGGGACGCAGTTACGATCGTTTCCGCGAGCGGGTGATGTTCCCCATTcgcg                                           <  1:1440059/150‑1 (MQ=255)
                                                                                                              gcgcCCCCCGGCTGGGACAACGTCCTGAAGCGGTTTGGCGGCAATCCAGAAAATCGCCAGTCATTGATTGATGCGGGGATGTTGGTCACTAACGATCAGGGACGCAGTTACGATCGTTTCCGCGAGCGGGTGATGTTCCCCATTCGCGAt                                         <  2:3075642/150‑1 (MQ=255)
                                                                                                               cgcCCCCCGTATGGGACAACGTCATGAATCGGTTTGGCGGCAATCCAGAAAATCGACAGTCATTGATTGATGCGGTGATGTTGGTCACTAACGATCAGGGACGCAGTTACGATAGTTTCCGCGAGCGGGTGATGTTCCCCATTAGCGATa                                        <  1:968149/150‑1 (MQ=255)
                                                                                                               cgcCCCCCGGCTGGGACAACGTCCTGAAGCGGTTTGGCGGCAATCCAGAAAATCGCCAGTCATTGATTGATGCGGGGATGTTGGTCACTAACGATCAGGGACGCAGTTACGATCGTTTCCGCGAGCGGGTGATGTTCCCCATTCGCGATa                                        <  1:967796/150‑1 (MQ=255)
                                                                                                               cgcCCCCCGGCTGGGACAACGTCCTGAAGCGGTTTGGCGGCAATCCAGAAAATCGCCAGTCATTGATTGATGCGGGGATGTTGGTCACTAACGATCAGGGACGCAGTTACGATCGTTTCCGCGAGCGGGTGATGTTCCCCATTCGCGATa                                        >  1:2909258/1‑150 (MQ=255)
                                                                                                               cgcCCCCCGGCTGGGACAACGTCCTGAAGCGGTTTGGCGGCAATCCAGAAAATCGCCAGTCATTGATTGATGCGGGGATGTTGGTCACTAACGATCAGGGACGCAGTTACGATCGTTTCCGCGAGCGGGTGATGTTCCCCATTCGCGATa                                        >  2:1701449/1‑150 (MQ=255)
                                                                                                                gcCCCCCGGCTGGGACAACGTCCTGAAGCGGTTTGGCGGCAATCCAGAAAATCGCCAGTCATTGATTGATGCGGGGATGTTGGTCACTAACGATCAGGGACGCAGTTACGATCGTTTCCGCGAGCGGGTGATGTTCCCCATTCGCGATaa                                       <  2:2420418/150‑1 (MQ=255)
                                                                                                                  cccccGGCTGGGACAACGTCCTGAAGCGGTTTGGCGGCAATCCAGAAAATCGCCAGTCATTGATTGATGCGGGGATGTTGGTCACTAACGATCAGGGACGCAGTTACGATCGTTTCCGCGAGCGGGTGATGTTCCCCATTCGCGATAAAc                                     >  1:967414/1‑150 (MQ=255)
                                                                                                                  cccccGGCTGGGACAACGTCCTGAAGCGGTTTGGCGGCAATCCAGAAAATCGCCAGTCATTGATTGATGCGGGGATGTTGGTCACTAACGATCAGGGACGCAGTTACGATCGTTTCCGCGAGCGGGTGATGTTCCCCATTCGCGATAAAc                                     >  1:2693450/1‑150 (MQ=255)
                                                                                                                      cGGCTGGGACAACGTCCTGAAGCGGTTTGGCGGCAATCCAGAAAATCGCCAGTCATTGATTGATGCGGGGATGTTGGTCACTAACGATCAGGGACGCAGTTACGATCGTTTCCGCGAGCGGGTGATGTTCCCCATTCGCGATAAACGCgg                                 <  1:3368523/150‑1 (MQ=255)
                                                                                                                        gCTGGGACAACGTCCTGAAGCGGTTTGGCGGCAATCCAGAAAATCGCCAGTCATTGATTGATGCGGGGATGTTGGTCACTAACGATCAGGGACGCAGTTACGATCGTTTCCGCGAGCGGGTGATGTTCCCCATTCGCGATAAACGCGGTc                               <  1:2615787/150‑1 (MQ=255)
                                                                                                                          tGGGACAACGTCCTGAAGCGGTTTGGCGGCAATCCAGAAAATCGCCAGTCATTGATTGATGCGGGGATGTTGGTCACTAACGATCAGGGACGCAGTTACGATCGTTTCCGCGAGCGGGTGATGTTCCCCATTCGCGATAAACGCGGTCgg                             >  1:3115833/1‑150 (MQ=255)
                                                                                                                           gggACAACGTCCTGAAGCGGTTTGGCTGCAATCCAGAAAATCGCCAGTCATTGATTGATGCGGGGATGTTGGTCACTAACGATCAGGGACGCAGTTACGATCGTTTCCGCGAGCGGGTGATGTTCCCCATTCGCGATAAACGCGGTCggg                            >  1:534363/1‑150 (MQ=255)
                                                                                                                            ggACAACGTCCTGAAGCGGTTTGGCGGCAATCCAGAAAATCGCCAGTCATTGATTGATGCGGGGATGTTGGTCACTAACGATCAGGGACGCAGTTACGATCGTTTCCGCGAGCGGGTGATGTTCCCCATTCGCGATAAACGCGGTCGGGt                           <  2:387728/150‑1 (MQ=255)
                                                                                                                             gACAACGTCCTGAAGCGGTTTGGCGGCAATCCAGAAAATCGCCAGTCATTGATTGATGCGGGGATGTTGGTCACTAACGATCAGGGACGCAGTTACGATCGTTTCCGCGAGCGGGTGATGTTCCCCATTCGCGATAAACGCGGTCGGGTg                          >  2:3983576/1‑150 (MQ=255)
                                                                                                                               caccGTCCTGAAGCGGTTTGGCGGCAATCCAGAAAATCGCCAGTCATTGATTGATGCGGGGATGTTGGTCACTAACGATCAGGGACGCAGTTACGATCGTTTCCGCGAGCGGGTGATGTTCCCCATTCGCGATAAACGCGGTCGGGTGAt                        <  2:2200837/147‑1 (MQ=255)
                                                                                                                                aaCGTCCTGAAGCGGTTTGGCGGCAATCCAGAAAATCGCCAGTCATTGATTGATGCGGGGATGTTGGTCACTAACGATCAGGGACGCAGTTACGATCGTTTCCGCGAGCGGGTGATGTTCACCATTCGCGATAAACGCGGTCGGGTGAtt                       >  1:301531/1‑150 (MQ=255)
                                                                                                                                 aCGTCCTGAAGCGGTTTGGCGGCAATCCAGAAAATCGCCAGTCATTGATTGATGCGGGGATGTTGGTCACTAACGATCAGGGACGCAGTTACGATCGTTTCCGCGAGCGGGTGATGTTCCCCATTCGCGATAAACGCGGTCGGGTGATTg                      <  1:143031/150‑1 (MQ=255)
                                                                                                                                  cGTCCTGAAGCGGTTTGGCGGCAATCCAGAAAATCGCCAGTCATTGATTGATGCGGGGATGTTGGTCACTAACGATCAGGGACGCAGTTACGATCGTTTCCGCGAGCGGGTGATGTTCCCCATTCGCGATAAACGCGGTCGGGTGATTgg                     >  1:3384543/1‑150 (MQ=255)
                                                                                                                                   gTCCTGAAGCGGTTTGGAGGCAATCCAGAAAATCGCCAGTCATTGATTGATGCGGGGATGTTGGTCACTAACGATCAGGGACGCAGTTACGATCGTTTCCGCGAGCGGGTGATGTTCCCCATTCGCGATAAACGCGGTCGGTTGATTGGt                    <  2:4270232/150‑1 (MQ=255)
                                                                                                                                    tCCTGAAGCGGTTTGGCGGCAATCCAGAAAATCGCCAGTCATTGATTGATGCGGGGATGTTGGTCACTAACGATCAGGGACGCAGTTACGATCGTTTCCGCGAGCGGGTGATGTTCCCCATTCGCGATAAACGCGGTCGGGTGATTGGtt                   >  1:1021924/1‑150 (MQ=255)
                                                                                                                                      cTGAAGCGGTTTGGCGGCAATCCAGAAAATCGCCAGTCATTGATTGATGCGGGGATGTTGGTCACTAACGATCAGGGACGCAGTTACGATCGTTTCCGCGAGCGGGTGATGTTCCCCATTCGCGATAAACGCGGTCGGGTGATTGGtttt                 >  1:2119723/1‑150 (MQ=255)
                                                                                                                                      cTGAAGCGGTGTGGCGGCAATCCAGAAACTCGCCAGTCATTGATTGATGCGGGGATGTTGGTCACTCACGATCAGGGACGCAGTTACGATCGTTTCCGCGAGCGGGTGATGTTCCCCATTCGCGATAACCGCGGTCGGGTGATTGGtttt                 <  2:3550100/150‑1 (MQ=255)
                                                                                                                                        gAAGCGGTTTGGCGGCAATCCAGAAAATCGCCAGTCATTGATTGATGCGGGGATGTTGGTCACTAACGATCAGGGACGCAGTTACGATCGTTTCCGCGAGCGGGTGATGTTCCCCATTCGCGATAAACGCGGTCGGGTGATTGGTTTTgg               <  2:4029518/150‑1 (MQ=255)
                                                                                                                                         aaGCGGTTTGGCGGCAATCCAGAAAATCGCCAGTCATTGATTGATGCGGGGATGTTGGTCACTAACGATCAGGGACGCAGTTACGATCGTTTCCGCGAGCGGGTGATGTTCCCCATTCGCGATAAACGCGGTCGGGTGATTGGTTTTggc              >  2:1563523/1‑150 (MQ=255)
                                                                                                                                         aaGCGGTTTGGCGGCAATCCAGAAAATCGCCAGTCATTGATTGATGCGGGGATGTTGGTCACTAACGATCAGGGACGCAGTTACGATCGTTTCCGCGAGCGGGTGATGTTCCCCATTCGCGATAAACGCGGTCGGGTGATTGGTTTTggc              >  2:1392387/1‑150 (MQ=255)
                                                                                                                                                 tGGCGGCAATCCAGAAAATCGCCAGTCATTGATTGATGCGGGGATGTTGGTCACTAACGATCAGGGACGCAGTTACGATCGTTTCCGCGAGCGGGTGATGTTCCCCATTCGCGATAAACGCGGTCGGGTGATTGGTTTTGGCGGGCGCGt      <  2:95323/150‑1 (MQ=255)
                                                                                                                                                  ggcggcAATCCAGAAAATCGCCAGTCATTGATTGATGCGGGGATGTTGGTCACTAACGATCAGGGACGCAGTTACGATCGTTTCCGCGAGCGGGTGATGTTCCCCATTCGCGATAAACGCGGTCGGGTGATTGGTTTTGGCGGGCGCGTg     >  2:412155/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
ATACGTTTTACCAACAATCTTTACAACAACCTGTTGCCACGTCTGCGCGCCAGTATCTGGAAAAACGCGGATTAAGCCACGAGGTTATCGCTCGCTTTGCGATTGGTTTTGCGCCCCCCGGCTGGGACAACGTCCTGAAGCGGTTTGGCGGCAATCCAGAAAATCGCCAGTCATTGATTGATGCGGGGATGTTGGTCACTAACGATCAGGGACGCAGTTACGATCGTTTCCGCGAGCGGGTGATGTTCCCCATTCGCGATAAACGCGGTCGGGTGATTGGTTTTGGCGGGCGCGTGCTG  >  CP009273/3204836‑3205134

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑