| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | CP009273 | 3,332,543 | C→A | 16.9% | R22L (CGG→CTG) | mlaE ← | ABC transporter maintaining OM lipid asymmetry, inner membrane permease protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | CP009273 | 3,332,543 | 0 | C | A | 16.9% | 894.6 / 123.9 | 298 | R22L (CGG→CTG) | mlaE | ABC transporter maintaining OM lipid asymmetry, inner membrane permease protein |
| Reads supporting (aligned to +/- strand): ref base C (97/150); new base A (16/35); total (113/185) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.43e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
Only 100 of 312 total aligned reads displayed.
CCCCAACACCATTCCGATGAACACGCCAGAAACCACAATAATCAGCATCGACAGGACGCCGACATTATAGAGCTGGCGCACCAGCAGCGGCGCATGTTTGCGAAATTCCGGTTTGCCGACCAGCGCATTGAATAACATTAACCCGGCCCGCCCGAACGTTCTCAGGGTTTTAATCCCTTTATGTCCGAGCGACGCCAGCGCATTTAACAGCATGAGTGGCTTAACTCCCTGGTAAAAGATCAGCGTGATAATCGCCGGCAGGATAGCGGAACGGAACAGGCCCGTCAGCTATCCCGT > CP009273/3332395‑3332691 | ccccAACACCATTCCGATGAACACGCCAGAAACCACAATAATCAGCATCGACAGGACGCCGACATTATAGAGCTGGCGCACCAGCAGCGGCGCATGTTTGCGAAATTCCGGTTTGCCGACCAGCGCATTGAATAACATTAACCCGgcccg < 1:544691/150‑1 (MQ=255) cccAACACCATTCCGATGAACACGCCAGAAACCACAATAATCAGCATCGACAGGACGCCGACATTATAGAGCTGGCGCACCAGCAGCGGCGCATGTTTGCGAAATTCCGGTTTGCCGACCAGCGCATTGAATAACATTAACCCGgcccgc > 1:1047699/1‑150 (MQ=255) cAACACCATTCCGATGAACACGCCAGAAACCACAATAATCAGCATCGACAGGACGCCGACATTATAGAGCTGGCGCACCAGCAGCGGCGCATGTTTGCGAAATTCCGGTTTGCCGACCAGCGCATTGAATAACATTAACCCGgcccgccc < 2:1022777/150‑1 (MQ=255) cAACACCATTCCGATGAACACGCCAGAAACCACAATAATCAGCATCGACAGGACGCCGACATTATAGAGCTGGCGCACCAGCAGCGGCGCATGTTTGCGAAATTCCGGTTTGCCGACCAGCGCATTGAATAACATTAACCCGgcccgccc < 1:1136120/150‑1 (MQ=255) aaCACCATTCCGATGAACACGCCAGAAACCACAATAATCAGCATCGACAGGACGCCGACATTATAGAGCTGGCGCACCAGCAGCGGCGCATGTTTGCGAAATTCCGGTTTGCCGACCAGCGCATTGAATAACATTAACCCGGcccgcccg > 1:4466627/1‑150 (MQ=255) acacCATTCCGATGAACACGCCAGAAACCACAATAATCAGCATCGACAGGACGCCGACATTATAGAGCTGGCGCACCAGCAGCGGCGCATGTTTGCGAAATTCCGGTTTGCCGACCAGCGCATTGAATAACATTAACCCGGCCCGCCCGa < 2:3316051/150‑1 (MQ=255) acacCATTCCGATGAACACGCCAGAAACCACAATAATCAGCATCGACAGGACGCCGACATTATAGAGCTGGCGCACCAGCAGCGGCGCATGTTTGCGAAATTCCGGTTTGCCGACCAGCGCATTGAATAACATTAACCCGGCCCGCCCGa > 1:3380552/1‑150 (MQ=255) acacCATTCCGATGAACACGCCAGAAACCACAAGAATCAGCATCGACAGGACGCCGACATTATAGAGCTGGCGCACCAGCAGCGGCGCATGTTTGCGAAATTCCGGTTTGCCGACCAGCGCATTGAATAACATTAACCCGGCCCGCCCGa < 2:1195883/150‑1 (MQ=255) ccATTCCGATGAACACGCCAGAAACCACAATAATCAGCATCGACAGGACGCCGACATTATAGAGCTGGCGCACCAGCAGCGGCGCATGTTTGCGAAATTCCGGTTTGCCGACCAGCGCATTGAATAACATTAACCCGGCCCGCCCGAACg < 2:2842899/150‑1 (MQ=255) aTTCCGATGAACACGCCAGAAACCACAATAATCAGCATCGACAGGACGCCGACATTATAGAGCTGGCGCACCAGCAGCGGCGCATGTTTGCGAAATTCCGGTTTGCCGACCAGCGCATTGAATAACATTAACCCGGCCCGCCCGAACGtt > 1:725029/1‑150 (MQ=255) aTTCCGATGAACACGCCAGAAACCACAAAAATCAGCATCGACAGGACGCCGACATTATAGAGCTGGCGCACCAGCAGCGGCGCATGTTTGCGAAATTCCGGTTTGCCGACCAGCGCAAAGAATAACATTAACCCGGCCCGCCCGAACGtt > 1:393138/1‑150 (MQ=255) tCCGATGAACACGCCAGAAACCACAATAATCAGCATCGACAGGACGCCGACATTATAGAGCTGGCGCACCAGCAGCGGCGCATGTTTGCGAAATTCCGGTTTGCCGACCAGCGCATTGAATAACATTAACCCGGCCCGCCCGAACGTtct < 2:2632402/150‑1 (MQ=255) tCCGATGAACACGCCAGAAACCACAATAATCAGCATCGACAGGACGCCGACATTATAGAGCTGGCGCACCAGCAGCGGCGCATGTTTGCGAAATTCCGGTTTGCCGACCAGCGCATTGAATAACATTAACCCGGCCAGCCCGAACGTtct < 2:2302281/150‑1 (MQ=255) ccGATGAACACGCCAGAAACCACAATAATCAGCATCGACAGGACGCCGACATTATAGAGCTGGCGCACCAGCAGCGGCGCATGTTTGCGAAATTCCGGTTTGCCGACCAGCGCATTGAATAACATTAACCCGGCCCGCCCGAACGTtctc < 1:3055773/150‑1 (MQ=255) gATGAACACGCCAGAAACCACAATAATCAGCATCGACAGGACGCCGACATTATAGAGCTGGCGCACCAGCAGCGGCGCATGTTTGCGAAATTCCGGTTTGCCGACCAGCGCATTGAATAACATTAACCCGGCCCGCCCGAACGTTCTCAg > 2:2125611/1‑150 (MQ=255) gATGAACACGCCAGAAACCACAATAATCAGCATCGACAGGACGCCGACATTATAGAGCTGGCGCACCAGCAGCGGCGCATGTTTGCGAAATTCCGGTTTGCCGACCAGCGCATTGAATAACATTAACCCGGCCCGCCCGAACGTTCTCAg < 1:948465/150‑1 (MQ=255) aTGAACACGCCAGAAACCACAATAATCAGCATCGACAGGACGCCGACATTATAGAGCTGGCGCACCAGCAGCGGCGCATGTTTGCGAAATTCCGGTTTGCCGACCAGCGCATTGAATAACATTAACCCGGCCCGCCCGAACGTTCTCAgg > 1:4063967/1‑150 (MQ=255) aTGAACACGCCAGAAACCACAATAATCAGCATCGACAGGACGCCGACATTATAGAGCTGGCGCACCAGCAGCGGCGCATGTTTGCGAAATTCCGGTTTGCCGACCAGCGCATTGAATAACATTAACCCGGCCAGCCCGAACGTTCTCAgg < 2:1010971/150‑1 (MQ=255) tGAACACGCCAGAAACCACAATAATCAGCATCGACAGGACGCCGACATTATAGAGCTGGCGCACCAGCAGCGGCGCATGTTTGCGAAATTCCGGTTTGCCGACCAGCGCATTGAATAACATTAACCCGGCCCGCCCGAACGTTCTCAggg > 2:1425831/1‑150 (MQ=255) acacGCCAGAAACCACAATAATCAGCATCGACAGGACGCCGACATTATAGAGCTGGCGCACCAGCAGCGGCGCATGTTTGCGAAATTCCGGTTTGCCGACCAGCGCATTGAATAACATTAACCCGGCCCGCCCGAACGTTCTCAGGGttt < 1:772756/150‑1 (MQ=255) cacGCCAGAAACCACAATAATCAGCATCGACAGGACGCCGACATTATAGAGCTGGCGCACCAGCAGCGGCGCATGTTTGCGAAATTCCGGTTTGCCGACCAGCGCATTGAATAACATTAACCCGGCCCGCCCGAACGTTCTCAGGGtttt < 2:578075/150‑1 (MQ=255) cacGCCAGAAACCACAATAATCAGCATCGACAGGACGCCGACATTATAGAGCTGGCGCACCAGCAGCGGCGCATGTTTGCGAAATTCCGGTTTGCCGACCAGCGCATTGAATAACATTAACCCGGCCAGCCCGAACGTTCTCAGGGtttt > 1:225150/1‑150 (MQ=255) acGCCAGAAACCACAATAATCAGCATCGACAGGACGCCGACATTATAGAGCTGGCGCACCAGCAGCGGCGCATGTTTGCGAAATTCCGGTTTGCCGACCAGCGCATTGAATAACATTAACCCGGCCCGCCCGAACGTTCTCAGGGTTTTa < 2:4392068/150‑1 (MQ=255) ccAGAAACCACAATAATCAGCATCGACAGGACGCCGACATTATAGAGCTGGCGCACCAGCAGCGGCGCATGTTTGCGAAATTCCGGTTTGCCGACCAGCGCATTGAATAACATTAACCCGGCCCGCCCGAACGTTCTCAGGGTTTTAATc < 1:1208002/150‑1 (MQ=255) ccAGAAACCACAATAATCAGCATCGACAGGACGCCGACATTATAGAGCTGGCGCACCAGCAGCGGCGCATGTTTGCGAAATTCCGGTTTGCCGACCAGCGCATTGAATAACATTAACCCGGCCCGCCCGAACGTTCTCAGGGTTTTAATc < 1:1714786/150‑1 (MQ=255) aGAAACCACAATAATCAGCATCGACAGGACGCCGACATTATAGAGCTGGCGCACCAGCAGCGGCGCATGTTTGCGAAATTCCGGTTTGCCGACCAGCGCATTGAATAACATTAACCCGGCCCGCCCGAACGTTCTCAGGGTTTTAATccc < 2:1010718/150‑1 (MQ=255) gAAACCACAATAATCAGCATCGACAGGACGCCGACATTATAGAGCTGGCGCACCAGCAGCGGCGCATGTTTGCGAAATACCGGTTTGCCGACCAGCGCATTGAATAACATTAACCCGGCCCGCCCGAACGTTCTCAGGGTTTTAATCCCt < 1:2258626/150‑1 (MQ=255) aaaCCACAATAATCAGCATCGACAGGACGCCGACATTATAGAGCTGGCGCACCAGCAGCGGCGCATGTTTGCGAAATTCCGGTTTGCCGACCAGCGCATTGAATAACATTAACCCGGCCCGCCCGAACGTTCTCAGGGTTTTAATCCCtt > 1:4122037/1‑150 (MQ=255) aaCCACAATAATCAGCATCGACAGGACGCCGACATTATAGAGCTGGCGCACCAGCAGCGGCGCATGTTTGCGAAATTCCGGTTTGCCGACCAGCGCATTGAATAACATTAACCCGGCCCGCCCGAACGTTCTCAGGGTTTTAATCCCttt > 2:770257/1‑150 (MQ=255) ccACAATAATCAGCATCGACAGGACGCCGACATTATAGAGCTGGCGCACCAGCAGCGGCGCATGTTTGCGAAATTCCGGTTTGCCGACCAGCGCATTGAATAACATTAACCCGGCCCGCCCGAACGTTCTCAGGGTTTTAATCCCTTTAt < 1:1975033/150‑1 (MQ=255) acaATACTCAGCATCGACAGGACGCCGACATTATAGAGCTGGCGCACCAGCAGCGGCGCATGTTTGCGAAATTCCGGTTTGCCGACCAGCGCATTGAATAACATTAACCCGGCCAGCCCGAACGTTCTCAGGGTTTTAATCCCTTTATGt < 2:3957829/150‑1 (MQ=255) acaATAATCAGCATCGACAGGACGCCGACATTATAGAGCTGGCGCACCAGCAGCGGCGCATGTTTGCGAAATTCCGGTTTGCCGACCAGCGCATTGAATAACATTAACCCGGCCCGCCCGAACGTTCTCAGGGTTTTAATCCCTTTATGt < 1:2812577/150‑1 (MQ=255) caATAATCAGCATCGACAGGACGCCGACATTATAGAGCTGGCGCACCAGCAGCGGCGCATGTTTGCGAAATTCCGGTTTGCCGACCAGCGCATTGAATAACATTAACCCGGCCCGCCCGAACGTTCTCAGGGTTTTAATCCCTTTATGTc > 2:1643189/1‑150 (MQ=255) caATAATCAGCATCGACAGGACGCCGACATTATAGAGCTGGCGCACCAGCAGCGGCGCATGTTTGCGAAATTCCGGTTTGCCGACCAGCGCATTGAATAACATTAACCCGGCCAGCCCGAACGTTCTCAGGGTTTTAATCCCTTTATGTc < 1:3293391/150‑1 (MQ=255) taatCAGCATCGACAGGACGCCGACATTATAGAGCTGGCGCACCAGCAGCGGCGCATGTTTGCGAAATTCCGGTTTGCCGACCAGCGCATTGAATAACATTAACCCGGCCAGCCCGAACGTTCTCAGGGTTTTAATCCCTTTATGTCCGa > 1:4400476/1‑150 (MQ=255) aatCAGCATCGACAGGACGCCGACATTATAGAGCTGGCGCACCAGCAGCGGCGCATGTTTGCGAAATTCCGGTTTGCCGACCAGCGCATTGAATAACATTAACCCGGCCCGCCCGAACGTTCTCAGGGTTTTAATCCCTTTATGTCCGAg > 2:334366/1‑150 (MQ=255) aatCAGCATCGACAGGACGCCGACATTATAGAGCTGGCGCACCAGCAGCGGCGCATGTTTGCGAAATTCCGGTTTGCCGACCAGCGCATTGAATAACATTAACCCGGCCCGCCCGAACGTTCTCAGGGTTTTAATCCCTTTATGTCCGAg < 1:4243733/150‑1 (MQ=255) atCAGCATCGACAGGACGCCGACATTATAGAGCTGGCGCACCAGCAGCGGCGCATGTTTGCGAAATTCCGGTTTGCCGACCAGCGCATTGAATAACATTAACCCGGCCCGCCCGAACGTTCTCAGGGTTTTAATCCCTTTATGTCCGAGc > 2:4166996/1‑150 (MQ=255) gCATCGACAGGACGCCGACATTATAGAGCTGGCGCACCAGCAGCGGCGCATGTTTGCGAAATTCCGGTTTGCCGACCAGCGCATTGAATAACATTAACCCGGCCCGCCCGAACGTTCTCAGGGTTTTAATCCCTTTATGTCCGAGCGACg < 1:2554455/150‑1 (MQ=255) gCATCGACAGGACGCCGACATTATAGAGCTGGCGCACCAGCAGCGGCGCATGTTTGCGAAATTCCGGTTTGCCGACCAGCGCATTGAATAACATTAACCCGGCCCGCCCGAACGTTCTCAGGGTTTTAATCCCTTTATGTCCGAGCGACg < 2:2034044/150‑1 (MQ=255) tCGACAGGACGCCGACATTATAGAGCTGGCGCACCAGCAGCGGCGCATGTTTGCGAAATTCCGGTTTGCCGACCAGCGCATTGAATAACATTAACCCGGCCCGCCCGAACGTTCTCAGGGTTTTAATCCCTTTATGTCCGAGCGACGCCa < 1:477520/150‑1 (MQ=255) tCGACAGGACGCCGACATTATAGAGCTGGCGCACCAGCAGCGGCGCATGTTTGCGAAATTCCGGTTTGCCGACCAGCGCATTGAATAACATTAACCCGGCCCGCCCGAACGTTCTCAGGGTTTTAATCCCTTTATGTCCGAGCGACGCCa < 1:1607204/150‑1 (MQ=255) cGCCGACATTATAGAGCTGGCGCACCAGCAGCGGCGCATGTTTGCGAAATTCCGGTTTGCCGACCAGCGCATTGAATAACATTACCCCGGCCCGCCCGAACGTTCTCAGGGTTTTAATCCCTTTATGTCCGAGCGACGCCAGCGCATTTa < 2:2481501/150‑1 (MQ=255) ataGAGCTGGCGCACCAGCAGCGGCGCATGTTTGCGAAATTCCGGTTTGCCGACCAGCGCATTGAATAACATTAACCCGGCCCGCCCGAACGTTCTCAGGGTTTTAATCCCTTTATGTCCGAGCGACGCCAGCGCATTTAACAGCATGAg > 2:3698267/1‑150 (MQ=255) ataGAGCTGGCGCACCAGCAGCGGCGCATGTTTGCGAAATTCCGGTTTGCCGACCAGCGCATTGAATAACATTAACCCGGCCCGCCCGAACGTTCTCAGGGTTTTAATCCCTTTATGTCCGAGCGACGCCAGCGCATTTAACAGCATGAg < 1:1657087/150‑1 (MQ=255) taGAGCTGGCGCACCAGCAGCGGCGCATGTTTGCGAAATTCCGGTTTGCCGACCAGCGCATTGAATAACATTAACCCGGCCCGCCCGAACGTTCTCAGGGTTTTAATCCCTTTATGTCCGAGCGACGCCAGCGCATTTAACAGCATGAGt < 1:4302436/150‑1 (MQ=255) taGAGCTGGCGCACCAGCAGCGGCGCATGTTTGCGAAATTCCGGTTTGCCGACCAGCGCATTGAATAACATTAACCCGGCCCGCCCGAACGTTCTCAGGGTTTTAATCCCTTTATGTCCGAGCGACGCCAGCGCATTTAACAGCATGAGt < 1:1341465/150‑1 (MQ=255) agagCTGGCGCACCAGCAGCGGCGCATGTTTGCGAAATTCCGGTTTGCCGACCAGCGCATTGAATAACATTAACCCGGCCCGCCCGAACGTTCTCAGGGTTTTAATCCCTTTATGTCCGAGCGACGCCAGCGCATTTAACAGCATGAGTg < 1:2054320/150‑1 (MQ=255) agCTGGCGCACCAGCAGCGGCGCATGTTTGCGAAATTCCGGTTTGCCGACCAGCGCATTGAATAACATTAACCCGGCCAGCCCGAACGTTCTCAGGGTTTTAATCCCTTTATGTCCGAGCGACGCCAGCGCATTTAACAGCATGAGTGGc < 2:3925091/150‑1 (MQ=255) agCTGGCGCACCAGCAGCGGCGCATGTTTGCGAAATTCCGGTTTGCCGACCAGCGCATTGAATAACATTAACCCGGCCAGCCCGAACGTTCTCAGGGTTTTAATCCCTTTATGTCCGAGCGACGCCAGCGCATTTAACAGCATGAGTGGc < 2:2328564/150‑1 (MQ=255) gCTGGCGCACCAGCAGCGGCGCATGTTTGCGAAATTCCGGTTTGCCGACCAGCGCATTGAATAACATTAACCCGGCCCGCCCGAACGTTCTCAGGGTTTTAATCCCTTTATGTCCGAGCGACGCCAGCGCATTTAACAGCATGAGTGGca < 1:1125075/150‑2 (MQ=255) cTGGCGCACCAGCAGCGGCGCATGTTTGCGAAATTCCGGTTTGCCGACCAGCGCATTGAATAACATTAACCCGGCCCGCCCGAACGTTCTCAGGGTTTTAATCCCTTTATGTCCGAGCGACGCCAGCGCATTTAACAGCATGAGTGGCtt < 2:3998208/150‑1 (MQ=255) tGGCGCACCAGCAGCGGCGCATGTTTGCGAAATTCCGGTTTGCCGACCAGCGCATTGAATAACATTAACCCGGCCCGCCCGAACGTTCTCAGGGTTTTAATCCCTTTATGTCCGAGCGACGCCAGCGCATTTAACAGCATGAGTGGCTTa < 2:2170570/150‑1 (MQ=255) tGGCGCACCAGCAGCGGCGCATGTTTGCGAAATTCCGGTTTGCCGACCAGCGCATTGAATAACATTAACCCGGCCCGCCCGAACGTTCTCAGGGTTTTAATCCCTTTATGTCCGAGCGACGCCAGCGCATTTAACAGCATGAGTGGCTTa < 2:4443160/150‑1 (MQ=255) tGGCGCACCAGCAGCGGCGCATGTTTGCGAAATTCCGGTTTGCCGACCAGCGCATTGAATAACATTAACCCGGCCCGCCCGAACGTTCTCAGGGTTTTAATCCCTTTATGTCCGAGCGACGCCAGCGCATTTAACAGCATGAGTGGCTTa < 2:1349050/150‑1 (MQ=255) ggCGCACCAGCAGCGGCGCATGTTTGCGAAATTCCGGTTTGCCGACCAGCGCATTGAATAACATTAACCCGGCCAGCCCGAACGTTCTCAGGGTTTTAATCCCTTTATGTCCGAGCGACGCCAGCGCATTTAACAGCATGAGTGGCTTaa < 1:2167416/150‑1 (MQ=255) cgcACCAGCAGCGGCGCATGTTTGCGAAATTCCGGTTTGCCGACCAGCGCATTGAATAACATTAACCCGGCCCGCCCGAACGTTCTCAGGGTTTTAATCCCTTTATGTCCGAGCGACGCCAGCGCATTTAACAGCATGAGTGGCTTAACt > 1:3233740/1‑150 (MQ=255) cgcACCAGCAGCGGCGCATGTTTGCGAAATTCCGGTTTGCCGACCAGCGCATTGAATAACATTAACCCGGCCCGCCCGAACGTTCTCAGGGTTTTAATCCCTTTATGTCCGAGCGACGCCAGCGCATTTAACAGCATGAGTGGCTTAACt < 1:2518146/150‑1 (MQ=255) cACCAGCAGCGGCGCATGTTTGCGAAATTCCGGTTTGCCGACCAGCGCATTGAATAACATTAACCCGGCCCGCCCGAACGTTCTCAGGGTTTTAATCCCTTTATGTCCGAGCGACGCCAGCGCATTTAACAGCATGAGTGGCTTAACTcc > 2:1795675/1‑150 (MQ=255) ccAGCAGCGGCGCATGTTTGCGAAATTCCGGTTTGCCGACCAGCGCATTGAATAACATTAACCCGGCCCGCCCGAACGTTCTCAGGGTTTTAATCCCTTTATGTCCGAGCGACGCCAGCGCATTTAACAGCATGAGTGGCTTAACTCCCt < 1:869487/150‑1 (MQ=255) agcagcGGCGCATGTTTGCGAAATTCCGGTTTGCCGACCAGCGCATTGAATAACATTAACCCGGCCCGCCCGAACGTTCTCAGGGTTTTAATCCCTTTATGTCCGAGCGACGCCAGCGCATTTAACAGCATGAGTGGCTTAACTCCCTgg < 1:3030919/150‑1 (MQ=255) agcagcGGCGCATGTTTGCGAAATTCCGGTTTGCCGACCAGCGCATTGAATAACATTAACCCGGCCCGCCCGAACGTTCTCAGGGTTTTAATCCCTTTATGTCCGAGCGACGCCAGCGCATTTAACAGCATGAGTGGCTTAACTCCCTgg < 2:1693389/150‑1 (MQ=255) gcATGTTTGCGAAATTCCGGTTTGCCGACCAGCTCATTGAATAACATTAACCCGGCCAGCCCGAACGTTCTCAGGGTTTTAATCCCTTTATGTCCGAGCGACGCCAGCGCATTTAACAGCATGAGTGGCTTAACTCCCTGGTAAAAGATc < 2:3110906/150‑1 (MQ=255) cATGTTTGCGAAATTCCGGTTTGCCGACCAGCGCATTGAATAACATTAACCCGGCCCGCCCGAACGTTCTCAGGGTTTTAATCCCTTTATGTCCGAGCGACGCCAGCGCATTTAACAGCATGAGTGGCTTAACTCCCTGGTAAAAGATCa < 2:3833824/150‑1 (MQ=255) tGTTTGCGAAATTCCGGTTTGCCGACCAGCGCATTGAATAACATTAACCCGGCCCGCCCGAACGTTCTCAGGGTTTTAATCCCTTTATGTCCGAGCGACGCCAGCGCATTTAACAGCATGAGTGGCTTAACTCCCTGGTAAAAGATCAGc < 2:4097031/150‑1 (MQ=255) tGTTTGCGAAATTCCGGTTTGCCGACCAGCGCATTGAATAACATTAACCCGGCCAGCCCGAACGTTCTCAGGGTTTTAATCCCTTTATGTCCGAGCGACGCCAGCGCATTTAACAGCATGAGTGGCTTAACTCCCTGGTAAAAGATCAGc < 1:353041/150‑1 (MQ=255) tGTTTGCGAAATTCCGGTTTGCCGACCAGCGCATTGAATAACATTAACCCGGCCAGCCCGAACGTTCTCAGGGTTTTAATCCCTTTATGTCCGAGCGACGCCAGCGCATTTAACAGCATGAGTGGCTTAACTCCCTGGTAAAAGATCAGc < 1:355486/150‑1 (MQ=255) ttGCGAAATTCCGGTTTGCCGACCAGCGCATTGAATAACATTAACCCGGCCCGCCCGAACGTTCTCAGGGTTTTAATCCCTTTATGTCCGAGCGACGCCAGCGCATTTAACAGCATGAGTGGCTTAACTCCCTGGTAAAAGATCAGCGTg < 2:1958483/150‑1 (MQ=255) gCGAAATTCCGGTTTGCCGACCAGCGCATTGAATAACATTAACCCGGCCCGCCCGAACGTTCTCAGGGTTTTAATCCCTTTATGTCCGAGCGACGCCAGCGCATTTAACAGCATGAGTGGCTTAACTCCCTGGTAAAAGATCAGCGTGAt < 1:3917354/150‑1 (MQ=255) gCGAAATTCCGGTTTGCCGACCAGCGCATTGAATAACATTAACCCGGCCCGCCCGAACGTTCTCAGGGTTTTAATCCCTTTATGTCCGAGCGACGCCAGCGCATTTAACAGCATGAGTGGCTTAACTCCCTGGTAAAAGATCAGCGTGAt < 1:3917434/150‑1 (MQ=255) cGAAATTCCGGTTTGCCGACCAGCGCATTGAATAACATTAACCCGGCCCGCCCGAACGTTCTCAGGGTTTTAATCCCTTTATGTCCGAGCGACGCCAGCGCATTTAACAGCATGAGTGGCTTAACTCCCTGGTAAAAGATCAGCGTGATa < 1:2757461/150‑1 (MQ=255) gAAATTCCGGTTTGCCGACCAGCGCATTGAATAACATTAACCCGGCCCGCCCGAACGTTCTCAGGGTTTTAATCCCTTTATGTCCGAGCGACGCCAGCGCATTTAACAGCATGAGTGGCTTAACTCCCTGGTAAAAGATCAGCGTGATaa < 1:2312300/150‑1 (MQ=255) gAAATTCCGGTTTGCCGACCAGCGCATTGAATAACATTAACCCGGCCCGCCCGAACGTTCTCAGGGTTTTAATCCCTTTATGTCCGAGCGACGCCAGCGCATTTAACAGCATGAGTGGCTTAACTCCCTGGTAAAAGATCAGCGTGATaa < 1:526537/150‑1 (MQ=255) aTTCCGGTTTGCCGACCAGCGCATTGAATAACATTAACCCGGCCCGCCCGAACGTTCTCAGGGTTTTAATCCCTTTATGTCCGAGCGACGCCAGCGCATTTAACAGCATGAGTGGCTTAACTCCCTGGTAAAAGATCAGCGTGATAATCg < 2:2726441/150‑1 (MQ=255) ttCCGGTTTGCCGACCAGCGCATTGAATAACATTAACCCGGCCCGCCCGAACGTTCTCAGGGTTTTAATCCCTTTATGTCCGAGCGACGCCAGCGCATTTAACAGCATGAGTGGCTTAACTCCCTGGTAAAAGATCAGCGTGATAATCGc > 2:258089/1‑150 (MQ=255) ccGGTTTGCCGACCAGCGCATTGAATAACATTAACCCGGCCCGCCCGAACGTTCTCAGGGTTTTAATCCCTTTATGTCCGAGCGACGCCAGCGCATTTAACAGCATGAGTGGCTTAACTCCCTGGTAAAAGATCAGCGTGATAATCGCCg < 1:3854388/150‑1 (MQ=255) ggTTTGCCGACCAGCGCATTGAATAACATTAACCCGGCCCGCCCGAACGTTCTCAGGGTTTTAATCCCTTTATGTCCGAGCGACGCCAGCGCATTTAACAGCATGAGTGGCTTAACTCCCTGGTAAAAGATCAGCGTGATAATCGCCGGc > 1:3704791/1‑150 (MQ=255) tttGCCGACCAGCGCATTGAATAACATTAACCCGGCCAGCCCGAACGTTCTCAGGGTTTTAATCCCTTTATGTCCGAGCGACGCCAGCGCAATTAACAGCATGAGTGGCTTAACTCCCTGGTAAAAGATCAGCGTGATAATCGCCGGCAg < 2:434984/150‑1 (MQ=255) ttGCCGACCAGCGCATTGAATAACATTAACCCGGCCCGCCCGAACGTTCTCAGGGTTTTAATCCCTTTATGTCCGAGCGACGCCAGCGCATTTAACAGCATGAGTGGCTTAACTCCCTGGTAAAAGATCAGCGTGATAATCGCCGGCAgg < 2:3471826/150‑1 (MQ=255) tGCCGACCAGCGCATTGAATAACATTAACCCGGCCAGCCCGAACGTTCTCAGGGTTTTAATCCCTTTATGTCCGAGCGACGCCAGCGCATTTAACAGCATGAGTGGCTTAACTCCCTGGTAAAAGATCAGCGTGATAATCGCCGGCAGGa < 1:2013797/150‑1 (MQ=255) ccGACCAGCGCATTGAATAACATTAACCCGGCCCGCCCGAACGTTCTCAGGGTTTTAATCCCTTTATGTCCGAGCGACGCCAGCGCATTTAACAGCATGAGTGGCTTAACTCCCTGGTAAAAGATCAGCGTGATAATCGCCGGCAGGATa < 1:1795818/150‑1 (MQ=255) aGCGCATTGAATAACATTAACCCGGCCCGCCCGAACGTTCTCAGGGTTTTAATCCCTTTATGTCCGAGCGACGCCAGCGCATTTAACAGCATGAGTGGCTTAACTCCCTGGTAAAAGATCAGCGTGATAATCGCCGGCAGGATAGcggaa > 2:3029253/1‑150 (MQ=255) aGCGCATTGAATAACATTAACCCGGCCCGCCCGAACGTTCTCAGGGTTTTAATCCCTTTATGTCCGAGCGACGCCAGCGCATTTAACAGCATGAGTGGCTTAACTCCCTGGTAAAAGATCAGCGTGATAATCGCCGGCAGGATAGcggaa < 1:1824080/150‑1 (MQ=255) aGCGCATTGAATAACATTAACCCGGCCCGCCCGAACGTTCTCAGGGTTTTAATCCCTTTATGTCCGAGCGACGCCAGCGCATTTAACAGCATGAGTGGCTTAACTCCCTGGTAAAAGATCAGCGTGATAATCGCCGGCAGGATAGcggaa > 2:2548840/1‑150 (MQ=255) cgcATTGAATAACATTAACCCGGCCCGCCCGAACGTTCTCAGGGTTTTAATCCCTTTATGTCCGAGCGACGCCAGCGCATTTAACAGCATGAGTGGCTTAACTCCCTGGTAAAAGATCAGCGTGATAATCGCCGGCAGGATAGcggaacg > 2:3529957/1‑150 (MQ=255) gcATTGAATAACATTAACCCGGCCCGCCCGAACGTTCTCAGGGTTTTAATCCCTTTATGTCCGAGCGACGCCAGCGCATTTAACAGCATGAGTGGCTTAACTCCCTGGTAAAAGATCAGCGTGATAATCGCCGGCAGGATAGcggaacgg < 1:1404066/150‑1 (MQ=255) gcATTGAATAACATTAACCCGGCCCGCCCGAACGTTCTCAGGGTTTTAATCCCTTTATGTCCGAGCGACGCCAGCGCATTTAACAGCATGAGTGGCTTAACTCCCTGGTAAAAGATCAGCGTGATAATCGCCGGCAGGATAGcggaacgg > 1:2424454/1‑150 (MQ=255) aTTGAATAACATTAACCCGGCCCGCCCGAACGTTCTCAGGGTTTTAATCCCTTTATGTCCGAGCGACGCCAGCGCATTTAACAGCATGAGTGGCTTAACTCCCTGGTAAAAGATCAGCGTGATAATCGCCGGCAGGATAGcggaacggaa < 2:4220802/150‑1 (MQ=255) ttGAATAACATTAACCCGGCCCGCCCGAACGTTCTCAGGGTTTTAATCCCTTTATGTCCGAGCGACGCCAGCGCATTTAACAGCATGAGTGGCTTAACTCCCTGGTAAAAGATCAGCGTGATAATCGCCGGCAGGATAGCggaacggaac < 1:1513534/150‑1 (MQ=255) tGAATAACATTAACCCGGCCCGCCCGAACGTTCTCAGGGTTTTAATCCCTTTATGTCCGAGCGACGCCAGCGCATTTAACAGCATGAGTGGCTTAACTCCCTGGTAAAAGATCAGCGTGATAATCGCCGGCAGGATAGCGGAACGGAACa < 2:2662121/150‑1 (MQ=255) tGAATAACATTAACCCGGCCCGCCCGAACGTTCTCAGGGTTTTAATCCCTTTATGTCCGAGCGACGCCAGCGCATTTAACAGCATGAGTGGCTTAACTCCCTGGTAAAAGATCAGCGTGATAATCGCCGGCAGGATAGCGGAACGGAACa < 2:1390990/150‑1 (MQ=255) aTAACATTAACCCGGCCCGCCCGAACGTTCTCAGGGTTTTAATCCCTTTATGTCCGAGCGACGCCAGCGCATTTAACAGCATGAGTGGCTTAACTCCCTGGTAAAAGATCAGCGTGATAATCGCCGGCAGGATAGCGGAACGGAACAGGc > 2:613833/1‑150 (MQ=255) aaCATTAACCCGGCCCGCCCGAACGTTCTCAGGGTTTTAATCCCTTTATGTCCGAGCGACGCCAGCGCATTTAACAGCATGAGTGGCTTAACTCCCTGGTAAAAGATCAGCGTGATAATCGCCGGCAGGATAGCGGAACGGAACAGGccc < 2:4298415/150‑1 (MQ=255) aaCATTAACCCGGCCCGCCCGAACGTTCTCAGGGTTTTAATCCCTTTATGTCCGAGCGACGCCAGCGCATTTAACAGCATGAGTGGCTTAACTCCCTGGTAAAAGATCAGCGTGATAATCGCCGGCAGGATAGCGGAACGGAACAGGccc > 1:1151496/1‑150 (MQ=255) ttAACCCGGCCCGCCCGAACGTTCTCAGGGTTTTAATCCCTTTATGTCCGAGCGACGCCAGCGCATTTAACAGCATGAGTGGCTTAACTCCCTGGTAAAAGATCAGCGTGATAATCGCCGGCAGGATAGCGGAACGGAACAGGCCCGTCa > 2:3603294/1‑150 (MQ=255) aaCCCGGCCAGCCCGAACGTTCTCAGGGTTTTAATCCCTTTATGTCCGAGCGACGCCAGCGCATTTAACAGCATGAGTGGCTTAACTCCCTGGTAAAAGATCAGCGTGATAATCGCCGGCAGGATAGCGGAACGGAACAGGCCCGTCAGc > 2:4406984/1‑150 (MQ=255) cGGCCCGCCCGAACGTTCTCAGGGTTTTAATCCCTTTATGTCCGAGCGACGCCAGCGCATTTAACAGCATGAGTGGCTTAACTCCCTGGTAAAAGATCAGCGTGATAATCGCCGGCAGGATAGCGGAACGGAACAGGCCCGTCAGCTATc > 2:2101792/1‑150 (MQ=255) ggCCCGCCCGAACGTTCTCAGGGTTTTAATCCCTTTATGTCCGAGCGACGCCAGCGCATTTAACAGCATGAGTGGCTTAACTCCCTGGTAAAAGATCAGCGTGATAATCGCCGGCAGGATAGCGGAACGGAACAGGCCCGTCAGCTATcc < 1:2125611/150‑1 (MQ=255) ggCCCGCCCGAACGTTCTCAGGGTTTTAATCCCTTTATGTCCGAGCGACGCCAGCGCATTTAACAGCATGAGTGGCTTAACTCCCTGGTAAAAGATCAGCGTGATAATCGCCGGCAGGATAGCGGAACGGAACAGGCCCGTCAGCTATcc < 2:1897542/150‑1 (MQ=255) ccgcccgAACGGTCTCAGGGTTTTAATCCCTTTATGTCCGAGCGACGCCAGCGCATTTAACAGCATGAGTGGCTTAACTCCCTGGTAAAAGATCAGCGTGATAATCGCCGGCAGGATAGCGGAACGGAACAGGCCCGTCAGCTATCCCGt > 2:723271/1‑150 (MQ=255) | CCCCAACACCATTCCGATGAACACGCCAGAAACCACAATAATCAGCATCGACAGGACGCCGACATTATAGAGCTGGCGCACCAGCAGCGGCGCATGTTTGCGAAATTCCGGTTTGCCGACCAGCGCATTGAATAACATTAACCCGGCCCGCCCGAACGTTCTCAGGGTTTTAATCCCTTTATGTCCGAGCGACGCCAGCGCATTTAACAGCATGAGTGGCTTAACTCCCTGGTAAAAGATCAGCGTGATAATCGCCGGCAGGATAGCGGAACGGAACAGGCCCGTCAGCTATCCCGT > CP009273/3332395‑3332691 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |