Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,365,527 C→T 12.0% S136N (AGC→AAC)  nanT ← sialic acid transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,365,5270CT12.0% 875.5 / 83.6 283S136N (AGC→AAC) nanTsialic acid transporter
Reads supporting (aligned to +/- strand):  ref base C (122/127);  new base T (24/10);  total (146/137)
Fisher's exact test for biased strand distribution p-value = 2.70e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 285 total aligned reads displayed.

ATGATTGGCAAAATGCCGATAAAGAACAGCGCACGCCAGCCCCAGACCGGAACCACCAGGCTATAGACCTGAGCGGCAACGACGGCCCCCACAGAGAAGCCTGAAATCAAAAAACCACTGGCTTTGTTACGCAGATGTTTTGGCCAGCTTTCAATGACATAGGTGGCGCTGGAACCGTATTCACCCGCCATCCCCATGCCGATGACCAGACGAGCGATAAACATGGTGATGTAGCCTGGCGCAAAGCCGCAGGCCAGCGTCCCGGCCGAGAAGAGAACGATGCTGGTGACCATTGCC  >  CP009273/3365380‑3365676
                                                                                                                                                   |                                                                                                                                                     
atgatTGGCAAAATGCCGATAAAGAACAGCGCACGCCAGCCCCAGACCGGAACCACCAGGCTATAGACCTGAGCGGCAACGACGGCCCCCACAGAGAAGCCTGAAATCAAAAAACCACTGGCTTTGTTACGCAGATGTTTTGGCCAGCtt                                                                                                                                                     >  1:842103/1‑150 (MQ=255)
     tGGCAAAATGCCGATAAAGAACAGCGCACGCCAGCCCCAGACCGGAACCACCAGGCTATAGACCTGAGCGGCAACGACGGCCCCCACAGAGAAGCCTGAAATCAAAAAACCACTGGCTTTGTTACGCAGATGTTTTGGCCAGTTTTCAAt                                                                                                                                                >  1:188193/1‑150 (MQ=255)
     tGGCAAAATGCCGATAAAGAACAGCGCACGCCAGCCCCAGACCGGAACCACCAGGCTATAGACCTGAGCGGCAACGACGGCCCCCACAGAGAAGCCTGAAATCAAAAAACCACTGGCTTTGTTACGCAGATGTTTTGGCCAGCTTTCAAt                                                                                                                                                >  2:31864/1‑150 (MQ=255)
      ggCAAAATGCCGATAAAGAACAGCGCACGCCAGCCCCAGACCGGAACCACCAGGCTATAGACCTGAGCGGCAACGACGGCCCCCACAGAGAAGCCTGAAATCAAAAAACCACTGGCTGTGTTACGCAGATGTTTTGGCCAGCTTTCAATg                                                                                                                                               >  2:2207659/1‑150 (MQ=255)
       gCAAAATGCCGATAAAGAACAGCGCACGCCAGCCCCAGACCGGAACCACCAGGCTATAGACCTGAGCGGCAACGACGGCCCCCACAGAGAAGCCTGAAATCAAAAAACCACTGGCTTTGTTACGCAGATGTTTTGGCCAGCTTTCAATGa                                                                                                                                              <  1:2255296/150‑1 (MQ=255)
        cAAAATGCCTATAAAGAACAGCGCACGCCCGCCCCAGACCGGAACCCCCCGGCTATAGACCTGAGCGGCAACGACGGCCCCCACAGAGAAGCCTGAAATCAAAAAACCACTGGCTTTGTTACGCAGATGTTTTGGCCAGCTTTCAATGAc                                                                                                                                             <  1:3645769/150‑1 (MQ=255)
         aaaaTGCCTATAAAGAACCGCGCACGCCACCCCCAGCCCGGAACCACCCGGCTTTAGCCCTGAGCGGAAACGACGGCCCCCACAGAGAAGCCTGAAATAAAAACACCACTGGCTTTGTTACGCAGATGTTTTGGCCAGTTTTCAATGACa                                                                                                                                            <  1:4214607/150‑1 (MQ=255)
         aaaaTGCCGATAAAGAACAGCGCACGCCAGCCCCAGACCGGAACCACCAGGCTATAGACCTGAGCGGCAACGACGGCCCCCACAGAGAAGCCTGAAATCAAAAAACCACTGGCTTTGTTACGCAGATGTTTTGGCCAGTTTTCAATGACa                                                                                                                                            <  1:3354406/150‑1 (MQ=255)
          aaaTGCCGATAAAGAACAGCGCACGCCAGCCCCAGACCGGAACCACCAGGCTATAGACCTGAGCGGCAACGACGGCCCCCACAGAGAAGCCTGAAATCAAAAAACCACTGGCTTTGTTACGCAGATGTTTTGGCCAGTTTTCAATGACAt                                                                                                                                           >  1:1582858/1‑150 (MQ=255)
          aaaTGCCGATAAAGAACAGCGCACGCCAGCCCCAGACCGGAACCACCAGGCTATAGACCTGAGCGGCAACGACGGCCCCCACAGAGAAGCCTGAAATCAAAAAACCACTGGCTTTGTTACGCAGATGTTTTGGCCAGCTTTCAATGACAt                                                                                                                                           >  2:2590164/1‑150 (MQ=255)
          aaaTGCCGATAAAGAACAGCGCACGCCAGCCCCAGACCGGAACCACCAGGCTATAGACCTGAGCGGCAACGACGGCCCCCACAGAGAAGCCTGAAATCAAAAAACCACTGGCTTTGTTACGCAGATGTTTTGGCCAGCTTTCAATGACAt                                                                                                                                           <  1:2644203/150‑1 (MQ=255)
           aaTGCCGATAAAGAACAGCGCACGCCAGCCCCAGACCGGAACCACCAGGCTATAGACCTGAGCGGCAACGACGGCCCCCACCGAGAAGCCTGAAATCAAAAAACCACTGGCTTTGTTACGCAGATGTTTTGGCCAGTTTTCAATGACATa                                                                                                                                          <  2:3817097/150‑1 (MQ=255)
               ccGATAAAGAACAGCGCACGCCAGCCCCAGACCGGAACCACCAGGCTATAGACCTGAGCGGCAACGACGGCCCCCACAGAGAAGCCTGAAATCAAAAAACCACTGGCTTTGTTACGCAGATGTTTTGGCCAGCTTTCAATGACATAGGTg                                                                                                                                      >  1:3888500/1‑150 (MQ=255)
                 gATAAAGAACAGCGCACGCCAGCCCCAGACCGGAACCACCAGGCTATAGACCTGAGCGGCAACGACGGCCCCCACAGAGAAGCCTGAAATCAAAAAACCACTGGCTTTGTTACGCAGATGTTTTGGCCAGTTTTCAATGACATAGGTGgc                                                                                                                                    >  1:1134965/1‑150 (MQ=255)
                  aTAAAGAACAGCGCACGCCAGCCCCAGACCGGAACCACCAGGCTATAGACCTGAGCGGCAACGACGGCCCCCACAGAGAAGCCTGAAATCAAAAAACCACTGGCTTTGTTACGCAGATGTTTTGGCCAGCTTTCAATGACATAGGTGgcg                                                                                                                                   >  1:4062176/1‑150 (MQ=255)
                  aTAAAGAACAGCGCACGCCAGCCCCAGACCGGAACCACCAGGCTATAGACCTGAGCGGCAACGACGGCCCCCACAGAGAAGCCTGAAATCAAAAAACCACTGGCTTTGTTACGCAGATGTTTTGGCCAGCTTTCAATGACATAGGTGgcg                                                                                                                                   >  1:3602872/1‑150 (MQ=255)
                   tAAAGAACAGCGCACGCCAGCCCCAGACCGGAACCACCAGGCTATAGACCTGAGCGGCAACGACGGCCCCCACAGAGAAGCCTGAAATCAAAAAACCACTGGCTTTGTTACGCAGATGTTTTGGCCAGCTTTCAATGACATAGGTGgcgc                                                                                                                                  >  1:3485295/1‑150 (MQ=255)
                     aaGAACAGCGCACGCCAGCCCCAGACCGGAACCACCAGGCTATAGACCTGAGCGGCAACGACGGCCCCCACAGAGAAGCCTGAAATCAAAAAACCACTGGCTTTGTTACGCAGATGTTTTGGCCAGCTTTCAATGACATAGGTGGCGCTg                                                                                                                                >  2:1899837/1‑150 (MQ=255)
                        aaCAGCGCACGCCAGCCCCAGACCGGAACCACCAGGCTATAGACCTGAGCGGCAACGACGGCCCCCACAGAGAAGCCTGAAATCAAAAAACCACTGGCTTTGTTACGCAGATGTTTTGGCCAGCTTTCAATGACATAGGTGGCGCTGGaa                                                                                                                             >  1:1328001/1‑150 (MQ=255)
                         aCAGCGCACGCCAGCCCCAGACCGGAACCACCAGGCTATAGACCTGAGCGGCAACGACGGCCCCCACAGAGAAGCCTGAAATCAAAAAACCACTGGCTTTGTTACGCAGATGTTTTGGCCAGCTTTCAATGACATAGGTGGCGCTGGAAc                                                                                                                            >  1:2192176/1‑150 (MQ=255)
                          cAGCGCACGCCAGCCCCAGACCGGAACCACCAGGCTATAGACCTGAGCGGCAACGACGGCCCCCACAGAGAAGCCTGAAATCAAAAAACCACTGGCTTTGTTACGCAGATGTTTTGGCCAGCTTTCAATGACATAGGTGGCGCTGGAAcc                                                                                                                           <  2:802372/150‑1 (MQ=255)
                           aGCGCACGCCAGCCCCAGACCGGAACCACCAGGCTATAGACCTGAGCGGCAACGACGGCCCCCACAGAGAAGCCTGAAATCAAAAAACCACTGGCTTTGTTACGCAGATGTTTTGGCCAGCTTTCAATGACATAGGTGGCGCTGGAACCg                                                                                                                          >  2:1436879/1‑150 (MQ=255)
                             cgcACGCCAGCCCCAGACCGGAACCACCAGGCTATAGACCTGAGCGGCAACGACGGCCCCCACAGAGAAGCCTGAAATCAAAAAACCACTGGCTTTGTTACGCAGATGTTTTGGCCAGCTTTCAATGACATAGGTGGCGCTGGAACCGTa                                                                                                                        <  2:4460360/150‑1 (MQ=255)
                              gcACGCCAGCCCCAGACCGGAACCACCAGGCTATAGACCTGAGCGGCAACGACGGCCCCCACAGAGAAGCCTGAAATCAAAAAACCACTGGCTTTGTTACGCAGATGTTTTGGCCAGCTTTCAATGACATAGGTGGCGCTGGAACCGTAt                                                                                                                       <  1:4140192/150‑1 (MQ=255)
                               cACGCCAGCCCCAGACCGGAACCACCAGGCTATAGACCTGAGCGGCAACGACGGCCCCCACAGAGAAGCCTGAAATCAAAAAACCACTGGCTTTGTTACGCAGATGTTTTGGCCAGCTTTCAATGACATAGGTGGCGCTGGAACCGTAtt                                                                                                                      >  1:2898990/1‑150 (MQ=255)
                               cACGCCAGCCCCAGACCGGAACCACCAGGCTATAGACCTGAGCGGCAACGACGGCCCCCACAGAGAAGCCTGAAATCAAAAAACCACTGGCTTTGTTACGCAGATGTTTTGGCCAGCTTTCAATGACATAGGTGGCGCTGGAACCGTAtt                                                                                                                      >  2:4320302/1‑150 (MQ=255)
                               cACGCCAGCCCCAGACCGGAACCACCAGGCTATAGACCTGAGCGGCAACGACGGCCCCCACAGAGAAGCCTGAAATCAAAAAACCACTGGCTTTGTTACGCAGATGTTTTGGCCAGCTTTCAATGACATAGGTGGCGCTGGAACCGTAtt                                                                                                                      <  2:1155049/150‑1 (MQ=255)
                               cACGCCAGCCCCAGACCGGAACCACCAGGCTATAGACCTGAGCGGCAACGACGGCCCCCACAGAGAAGCCTGAAATCAAAAAACCACTGGCTTTGTTACGCAGATGTTTTGGCCAGCTTTCAATGACATAGGTGGCGCTGGAACCGTAtt                                                                                                                      >  1:2208043/1‑150 (MQ=255)
                                aCGCCAGCCCCAGACCGGAACCACCAGGCTATAGACCTGAGCGGCAACGACGGCCCCCACAGAGAAGCCTGAAATCAAAAAACCACTGGCTTTGTTACGCAGATGTTTTGGCCAGCTTTCAATGACATAGGTGGCGCTGGAACCGTATTc                                                                                                                     >  2:1082907/1‑150 (MQ=255)
                                   ccAGCCCCAGACCGGAACCACCAGGCTATAGACCTGAGCGGCAACGACGGCCCCCACAGAGAAGCCTGAAATCAAAAAACCACTGGCTTTGTTACGCAGATGTTTTGGCCAGCTTTCAATGACATAGGTGGCGCTGGAACCGTATTCAcc                                                                                                                  >  1:3129384/1‑150 (MQ=255)
                                    cAGCCCCAGACCGGAACCACCAGGCTATAGACCTGAGCGGCAACGACGGCCCCCACAGAGAAGCCTGAAATCAAAAAACCACTGGCTTTGTTACGCAGATGTTTTGGCCAGCTTTCAATGACATAGGTGGCGCTGGAACCGTATTCAccc                                                                                                                 <  2:4294772/150‑1 (MQ=255)
                                     aGCCCCAGACCGGAACCACCAGGCTATAGACCTGAGCGGCAACGACGGCCCCCACAGAGAAGCCTGAAATCAAAAAACCACTGGCTTTGTTACGCAGATGTTTTGGCCAGCTTTCAATGACATAGGTGGCGCTGGAACCGTATTCACCCg                                                                                                                >  1:99015/1‑150 (MQ=255)
                                          cAGACCGGAACCACCAGGCTATAGACCTGAGCGGCAACGACGGCCCCCACAGAGAAGCCTGAAATCAAAAAACCACTGGCTTTGTTACGCAGATGTTTTGGCCAGCTTTCAATGACATAGGTGGCGCTGGAACCGTATTCACCCGCCATc                                                                                                           >  1:1648003/1‑150 (MQ=255)
                                           aGACCGGAACCACCAGGCTATAGACCTGAGCGGCAACGACGGCCCCCACATAGTAGCATGAAATCAAAAAACCACTGGCTTTGTTACGCAGATGTTTTGGCCAGCTTTCAATGACATAGGTGGCGCTGGAACCGTATTCACCCGCCATcc                                                                                                          <  2:1752878/150‑1 (MQ=255)
                                           aGACCGGAACCACCAGGCTATAGACCTGAGCGGCAACGACGGCCCCCACAGAGAAGCCTGAAATCAAAAAACCACTGGCTTTGTTACGCAGATGTTTTGGCCAGCTTTCAATGACATAGGTGGCGCTGGAACCGTATTCACCCGCCATcc                                                                                                          >  2:2179260/1‑150 (MQ=255)
                                             aCCGGAACCACCAGGCTATAGACCTGAGCGGCAACGACGGCCCCCACAGAGAAGCCTGAAATCAAAAAACCACTGGCTTTGTTACGCAGATGTTTTGGCCAGCTTTCAATGACATAGGTGGCGCTGGAACCGTATTCACCCGCCATcccc                                                                                                        <  2:3940059/150‑1 (MQ=255)
                                              ccGGAACCACCAGGCTATAGACCTGAGCGGCAACGACGGCCCCCACAGAGAAGCCTGAAATCAAAAAACCACTGGCTTTGTTACGCAGATGTTTTGGCCAGCTTTCAATGACATAGGTGGCGCTGGAACCGTATTCACCCGCCATCCCCa                                                                                                       >  2:76075/1‑150 (MQ=255)
                                                        caGGATATAGACGTTAGCGGCAACTACGGCCCCCACATATAATCCTTAAATCAAAAAACCACTGGCTTTGTTACGCAGATGTTTTGGCCAGCTTTCAATGACATAGGTGGCGCTGGAACCGTATTCACCCGCCATCCCCATGCCGATGAc                                                                                             <  1:3165887/150‑1 (MQ=255)
                                                            cTATAGACCTGAGCGGCAACGACGGCCCCCACAGAGAAGCCTGAAATCAAAAAACCACTGGCTTTGTTACGCAGATGTTTTGGCCAGTTTTCAATGACATAGGTGGCGCTGGAACCGTATTCACCCGCCATCCCCATGCCGATGACCAGa                                                                                         >  1:3307371/1‑150 (MQ=255)
                                                            atataGACCTGAGCGGAAACGACGGCCCCCACAGATAAGCATTAAAACAAAAAACCACTGGCTTTGTTACGCAGATGTTTTGGCCAGCTTTCAATGACATAGGTGGCGCTGGAACCGTATTCACCCGCCATCCCCATGCCGATGACCAGa                                                                                         <  1:3034329/149‑1 (MQ=255)
                                                                  aCCTGAGCGGCAACGACGGCCCCCACAGAGAAGCCTGAAATAAAAAAACCACTGGCTTTGTTACGCAGATGTTTTGGCCAGCTTTCAATGACATAGGTGGCGCTGGAACCGTATTCACCCGCCATCCCCATGCCGATGACCAGACGAGCg                                                                                   <  2:3493966/150‑1 (MQ=255)
                                                                     tGAGCGGCAACGACGGCCCCCACAGAGAAGCCTGAAATCAAAAAACCACTGGCTTTGTTACGCAGATGTTTTGGCCAGTTTTCAATGACATAGGTGGCGCTGGAACCGTATTCACCCGCCATCCCCATGCCGATGACCAGACGAGCGATa                                                                                >  2:984397/1‑150 (MQ=255)
                                                                     tGAGCGGCAACGACGGCCCACACAGAGAAGCATTAAATAAAAAAACCACTGGCTTTGTTACGCAGATGTTTTGGCCAGCTTTCAATGACATAGGTGGCGCTGGAACCGTATTCACCCGCCATCCCCATGCCGATGACCAGACGAGCGATa                                                                                <  1:3583174/150‑1 (MQ=255)
                                                                        gCGGCAACGACGGCCCCCACAGAGAAGCCTGAAATAAAAACACCACTGGCTTTGTTACGCAGATGTTTTGGCCAGCTTTCAATGACATAGGTGGCGCTGGAACCGTATTCACCCGCCATCCCCATGCCGATGACCAGACGAGCGATAAAc                                                                             <  1:3425146/150‑1 (MQ=255)
                                                                        gCGGCAACGACGGCCCACACAGAGAAGCCTGAAATCAAAAAACCACTGGCTTTGTTACGCAGATGTTTTGGCCAGCTTTCAATGACATAGGTGGCGCTGGAACCGTATTCACCCGCCATCCCCATGCCGATGACCAGACGAGCGATAAAc                                                                             <  2:2097924/150‑1 (MQ=255)
                                                                         cGGCAACGACGGCCCCCACAGAGAAGCCTGAAATCAAAAAACCACTGGCTTTGTTACGCAGATGTTTTGGCCAGCTTTCAATGACATAGGTGGCGCTGGAACCGTATTCACCCGCCATCCCCATGCCGATGACCAGACGAGCGATAAACa                                                                            <  1:1103671/150‑1 (MQ=255)
                                                                             aaCGACGGCCCCCACAGAGAAGCCTGAAATAAAAAAACCACTGGCTTTGTTACGCAGATGTTTTGGCCAGCTTTCAATGACATAGGTGGCGCTGGAACCGTATTCACCCGCCATCCCCATGTCGATGACCAGACGAGCGATAAACATGGt                                                                        <  1:1229764/150‑1 (MQ=255)
                                                                              acgacgGCCCCCACAGAGAAGCTTGAAATCAAAAAACCACTGGCTTTGTTACGCAGATGTTTTGGCCAGCTTTCAATGACATAGGTGGCGCTGGAACCGTATTCACCCGCCATCCCCATGCCGATGACCAGACGAGCGATAAAGATGGtt                                                                       <  2:1719643/150‑2 (MQ=255)
                                                                                gacgGCCCCCACAGATAAGCCTGAAATCAAAAAACCACTGGCTTTGTTACGCAGATGTTTTGGCCAGCTTTCAATGACATAGGTGGCGCTGGAACCGTATTCACCCGCCATCCCCATGCCGATGACCAGACGAGCGATAAACATGGTTAt                                                                     <  2:542425/150‑1 (MQ=255)
                                                                                gacgGCCCCCACAGAGAAGCCTGAAATCAAAAAACCACTGGCTTTGTTACGCAGATGTTTTGGCCAGCTTTCAATGACATAGGTGGCGCTGGAACCGTATTCACCCGCCATCCCCATGCCGATGACCAGACGAGCGATAAACATGGTTAt                                                                     >  2:3444208/1‑150 (MQ=255)
                                                                                gacgGCCCCCACAGAGAAGCCTGAAATCAAAAAACCACTGGCTTTGTTACGCAGATGTTTTGGCCAGCTTTCAATGACATAGGTGGCGCTGGAACCGTATTCACCCGCCATCCCCATGCCGATGACCAGACGAGCGATAAACATGGTGAt                                                                     >  2:2872282/1‑150 (MQ=255)
                                                                                  cgGCCCCCACAGAGAAGCCTGAAATCAAAAAACCACTGGCTTTGTTACGCAGATGTTTTGGCCAGCTTTCAATGACATAGGTGGCGCTGGAACCGTATTCACCCGCCATCCCCATGCCGATGACCAGACGAGCGATAAACATGGttttgt                                                                   <  2:1328001/150‑6 (MQ=255)
                                                                                  cgGCCCCCACAGAGAAGCCTGAAATCAAAAAACCACTGGCTTTGTTACGCAGATGTTTTGGCCAGCTTTCAATGACATAGGTGGCGCTGGAACCGTATTCACCCGCCATCCCCATGCCGATGACCAGACGAGCGATAAACATGGTTATGt                                                                   <  1:1436879/150‑1 (MQ=255)
                                                                                  cgGCCCCCACAGAGAAGCCTGAAATCAAAAAACCACTGGCTTTGTTACGCAGATGTTTTGGCCAGCTTTCAATGACATAGGTGGCGCTGGAACCGTATTCACCCGCCATCCCCATGCCGATGACCAGACGAGCGATAAACATGGTTATGt                                                                   <  1:1726501/150‑1 (MQ=255)
                                                                                  cgGCCCCCACAGAGAAGCCTGAAATCAAAAAACCACTGGCTTTGTTACGCAGATGTTTTGGCCAGCTTTCAATGACATAGGTGGCGCTGGAACCGTATTCACCCGCCATCCCCATGCCGATGACCAGACGAGCGATAAACATGGTTATGt                                                                   <  1:3771625/150‑1 (MQ=255)
                                                                                   ggCCCCCACAGAGAAGCCTGAAATCAAAAAACCACTGGCTTTGTTACGCAGATGTTTTGGCCAGCTTTCAATGACATAGGTGGCGCTGGAACCGTATTCACCCGCCATCCCCATGCCGATGACCAGACGAGCGATAAACATGGTTATGTa                                                                  <  2:533527/150‑1 (MQ=255)
                                                                                    gCCCCCACAGAGAAGCCTTAAATCAAAAAACCACTGGCTTTGTTACGCAGATGTTTTGGCCAGCTTTCAATGACATAGGTGGCGCTGGAACCGTATTCACCCGCCATCCCCATGCCGATGACCAGACGAGCGATAAACATGGTGATGTAg                                                                 <  1:2422665/150‑1 (MQ=255)
                                                                                    gCCCCCACAGAGAAGCCTGAAATCAAAAAACCACTGGCTTTGTTACGCAGATGTTTTGGCCAGCTTTCAATGACATAGGTGGCGCTGGAACCGTATTCACCCGCCATCCCCATGCCGATGACCAGACGAGCGATAAACATGGTTATGTAg                                                                 <  2:3485295/150‑1 (MQ=255)
                                                                                     cccccACAGAGAAGCCTGAAATCAAAAAACCACTGGCTTTGTTACGCAGATGTTTTGGCCAGCTTTCAATGACATAGGTGGCGCTGGAACCGTATTCACCCGCCATCCCCATGCCGATGACCAGACGAGCGATAAACATGGTTATGTAGc                                                                >  1:4412223/1‑150 (MQ=255)
                                                                                      ccccACAGAGAAGCCTGAAATCAAAAAACCACTGGCTTTGTTACGCAGATGTTTTGGCCAGTTTTCAATGACATAGGTGGCGCTGGAACCGTATTCACCCGCCATCCCCATGCCGATGACCAGACGAGCGATAAACATGGTTATGTAGcc                                                               >  2:2570819/1‑150 (MQ=255)
                                                                                      ccccACAGAGAAGCCTGAAATCAAAAAACCACTGGCTTTGTTACGCAGATGTTTTGGCCAGCTTTCAATGACATAGGTGGCGCTGGAACCGTATTCACCCGCCATCCCCATGCCGATGACCAGACGAGCGATAAACATGGTTATGTAGcc                                                               <  1:4258556/150‑1 (MQ=255)
                                                                                       cccACAGAGAAGCCTGAAATCAAAAAACCACTGGCTTTGTTACGCAGATGTTTTGGCCAGCTTTCAATGACATAGGTGGCGCTGGAACCGTATTCACCCGCCATCCCCATGCCGATGACCAGACGAGCGATAAACATGGTTATGTAGCCt                                                              <  2:4089046/150‑1 (MQ=255)
                                                                                             gagaAGCCTGAAAACAAAAAACCACTGGCTTTGTTACGCAGATGTTTTGGCCAGCTTTCAATGACATAGGTGGCGCTGGAACCGTATTCACCCGCCATCCCCATGCCGATGACCAGACGAGCGATAAACATGGTTATGTAGCCTGGCGCa                                                        <  1:897587/150‑1 (MQ=255)
                                                                                              agaAGCCTGAAATCAAAAAACCACTGGCTTTGTTACGCAGATGTTTTGGCCAGCTTTCAATGACATAGGTGGCGCTGGAACCGTATTCACCCGCCATCCCCATGCCGATGACCAGACGAGCGATAAACATGGTGATGTAGCCTGGCGCaa                                                       >  1:2091706/1‑150 (MQ=255)
                                                                                                 aGCCTGAAATCAAAAAACCACTGGCTTTGTTACGCAGATGTTTTGGCCAGCTTTCAATGACATAGGTGGCGCTGGAACCGTATTCACCCGCCATCCCCATGCCGATGACCAGACGAGCGATAAACATGGTGATGTAGCCTGGCGCAAAGc                                                    <  2:4149653/150‑1 (MQ=255)
                                                                                                     tGAAATCAAAAAACCACTGGCTTTGTTACGCAGATGTTTTGGCCAGCTTTCAATGACATAGGTGGCGCTGGAACCGTATTCACCCGCCATCCCCATGCCGATGACCAGACGAGCGATAAACATGGTTATGTAGCCTGGCGCAAAGCCGCa                                                >  2:3104442/1‑150 (MQ=255)
                                                                                                      gAAATCAAAAAACCACTGGCTTTGTTACGCAGATGTTTTGGCCAGTTTTCAATGACATAGGTGGCGCTGGAACCGTATTCACCCGCCATCCCCATGCCGATGACCAGACGAGCGATAAACATGGTTATGTAGCCTGGCGCAAAGCCGCAg                                               >  2:3470324/1‑150 (MQ=255)
                                                                                                          tCAAAAAACCACTGGCTTTGTTACGCAGATGTTTTGGCCAGCTTTCAATGACATAGGTGGCGCTGGAACCGTATTCACCCGCCATCCCCATGCCGATGACCAGACGAGCGATAAACATGGTTATGTAGCCTGGCGCAAAGCCGCAGGCCa                                           <  1:605856/150‑1 (MQ=255)
                                                                                                           cAAAAAACCACTGGCTTTGTTACGCAGATGTTTTGGCCAGTTTTCAATGACATAGGTGGCGCTGGAACCGTATTCACCCGCCATCCCCATGCCGATGACCAGACGAGCGATAAACATGGTTATGTAGCCTGGCGCAAAGCCGCAGGCCAg                                          <  1:3968211/150‑1 (MQ=255)
                                                                                                           cAAAAAACCACTGGCTTTGTTACGCAGATGTTTTGGCCAGCTTTCAATGACATAGGTGGCGCTGGAACCGTATTCACCCGCCATCCCCATGCCGATGACCAGACGAGCGATAAACATGGTTATGTAGCCTGGCGCAAAGCCGCAGGCCAg                                          <  1:856466/150‑1 (MQ=255)
                                                                                                           cAAAAAACCACTGGCTTTGTTACGCAGATGTTTTGGCCAGCTTTCAATGACATAGGTGGCGCTGGAACCGTATTCACCCGCCATCCCCATGCCGATGACCAGACGAGCGATAAACATGGTTATGTAGCCTGGCGCAAAGCCGCAGGCCAg                                          <  1:609161/150‑1 (MQ=255)
                                                                                                            aaaaaaCCACTGGCTTTGTTACGCAGATGTTTTGGCCAGCTTTCAATGACATAGGTGGCGCTGGAACCGTATTCACCCGCCATCCCCATGCCGATGACCAGACGAGCGATAAACATGGTTATGTAGCCTGGCGCAAAGCCGCAGGCCAGc                                         >  1:1021262/1‑150 (MQ=255)
                                                                                                             aaaaaCCACTGGCTTTGTTACGCAGATGTTTTGGCCAGCTTTCAATGACATAGGTGGCGCTGGAACCGTATTCACCCGCCATCCCCATGCCGATGACCAGACGAGCGATAAACATGGTTATGTAGCCTGGCGCAAAGCCGCAGGCCAGCg                                        >  2:2769930/1‑150 (MQ=255)
                                                                                                              aaaaCCACTGGCTTTGTTACGCAGATGTTTTGGCCAGCTTTCAATGACATAGGTGGCGCTGGAACCGTATTCACCCGCCATCCCCATGCCGATGACCAGACGAGCGATAAACATGGTTATGTAGCCTGGCGCAAAGCCGCAGGCCAGCGt                                       >  2:2960133/1‑150 (MQ=255)
                                                                                                               aaaCCACTGGCTTTGTTACGCAGATGTTTTGGCCAGCTTTCAATGACATAGGTGGCGCTGGAACCGTATTCACCCGCCATCCCCATGCCGATGACCAGACGAGCGATAAACATGGTTATGTAGCCTGGCGCAAAGCCGCAGGCCAGCGTc                                      <  2:2192176/150‑1 (MQ=255)
                                                                                                                aaCCACTGGCTTTGTTACGCAGATGTTTTGGCCAGCTTTCAATGACATAGGTGGCGCTGGAACCGTATTCACCCGCCATCCCCATGCCGATGACCAGACGAGCGATAAACATGGTTATGTAGCCTGGCGCAAAGCCGCAGGCCAGCGTcc                                     >  2:1651518/1‑150 (MQ=255)
                                                                                                                   cACTGGCTTTGTTACGCAGATGTTTTGGCCAGCTTTCAATGACATAGGTGGCGCTGGAACCGTATTCACCCGCCATCCCCATGCCGATGACCAGACGAGCGATAAACATGGTTATGTAGCCTGGCGCAAAGCCGCAGGCCAGCGTCCCgg                                  >  1:1567698/1‑150 (MQ=255)
                                                                                                                   cACTGGCTTTGTTACGCAGATGTTTTGGCCAGCTTTCAATGACATAGGTGGCGCTGGAACCGTATTCACCCGCCATCCCCATGCCGATGACCAGACGAGCGATAAACATGGTTATGTAGCCTGGCGCAAAGCCGCAGGCCAGCGTCCCgg                                  <  1:2601758/150‑1 (MQ=255)
                                                                                                                        gCTTTGTTACGCAGATGTTTTGGCCAGCTTTCAATGACATAGGTGGCGCTGGAACCGTATTCACCCGCCATCCCCATGCCGATGACCAGACGAGCGATAAACATGGTTATGTAGCCTGGCGCAAAGCCGCAGGCCAGCGTCCCGGCCgag                             >  2:152020/1‑150 (MQ=255)
                                                                                                                         cTTTGTTACGCAGATGTTTTGGCCAGCTTTCAATGACATAGGTGGCGCTGGAACCGTATTCACCCGCCATCCCCATGCCGATGACCAGACGAGCGATAAACATGGTTATGTAGCCTGGCGCAAAGCCGCAGGCCAGCGTCCCGGCCgaga                            >  2:3375433/1‑150 (MQ=255)
                                                                                                                         cTTTGTTACGCAGATGTTTTGGCCAGCTTTCAATGACATAGGTGGCGCTGGAACCGTATTCACCCGCCATCCCCATGCCGATGACCAGACGAGCGATAAACATGGTTATGTAGCCTGGCGCAAAGCCGCAGGCCAGCGTCCCGGCCgaga                            >  2:2322338/1‑150 (MQ=255)
                                                                                                                         cTTTGTTACGCAGATGTTTTGGCCAGCTTTCAATGACATAGGTGGCGCTGGAACCGTATTCACCCGCCATCCCCATGCCGATGACCAGACGAGCGATAAACATGGTTATGTAGCCTGGCGCAAAGCCGCAGGCCAGCGTCCCGGCCgaga                            >  2:4098320/1‑150 (MQ=255)
                                                                                                                          tttGTTACGCAGATGTTTTGGCCAGCTTTCAATGACATAGGTGGCGCTGGAACCGTATTCACCCGCCATCCCCATGCCGATGACCAGACGAGCGATAAACATGGTTATGTAGCCTGGCGCAAAGCCGCAGGCCAGCGTCCCGGCCgagaa                           >  1:2073862/1‑150 (MQ=255)
                                                                                                                          tttGTTACGCAGATGTTTTGGCCAGCTTTCAATGACATAGGTGGCGCTGGAACCGTATTCACCCGCCATCCCCATGCCGATGACCAGACGAGCGATAAACATGGTTATGTAGCCTGGCGCAAAGCCGCAGGCCAGCGTCCCGGCCgagaa                           <  1:3319074/150‑1 (MQ=255)
                                                                                                                          tttGTTACGCAGATGTTTTGGCCAGCTTTCAATGACATAGGTGGCGCTGGAACCGTATTCACCCGCCATCCCCATGCCGATGACCAGACGAGCGATAAACATGGTTATGTAGCCTGGCGCAAAGCCGCAGGCCAGCGTCCCGGCCgagaa                           <  1:2830618/150‑1 (MQ=255)
                                                                                                                             gTTACGCAGATGTTTTGGCCAGCTTTCAATGACATAGGTGGCGCTGGAACCGTATTCACCCGCCATCCCCATGCCGATGACCAGACGAGCGATAAACATGGTTATGTAGCCTGGCGCAAAGCCGCAGGCCAGCGTCCCGGCCgagaagag                        >  2:956426/1‑150 (MQ=255)
                                                                                                                              ttACGCAGATGTTTTGGCCAGCTTTCAATGACATAGGTGGCGCTGGAACCGTATTCACCCGCCATCCCCATGCCGATGACCAGACGAGCGATAAACATGGTGATGTAGCCTGGCGCAAAGCCGCAGGCCAGCGTCCCGGCCgagaagaga                       >  1:1350583/1‑150 (MQ=255)
                                                                                                                               tACGCAGATGTTTTGGCCAGCTTTCAATGACATAGGTGGCGCTGGAACCGTATTCACCCGCCATCCCCATGCCGATGACCAGACGAGCGATAAACATGGTTATGTAGCCTGGCGCAAAGCCGCAGGCCAGCGTCCCGGCCgagaagagaa                      >  1:27578/1‑150 (MQ=255)
                                                                                                                                  gCAGATGTTTTGGCCAGCTTTCAATGACATAGGTGGCGCTGGAACCGTATTCACCCGCCATCCCCATGCCGATGACCAGACGAGCGATAAACATGGTTATGTAGCCTGGCGCAAAGCCGCAGGCCAGCGTCCCGGCCGAGAAGAGAACGa                   >  2:2907311/1‑150 (MQ=255)
                                                                                                                                   cAGATGTTTTGGCCAGCTTTCAATGACATAGGTGGCGCTGGAACCGTATTCACCCGCCATCCCCATGCCGATGACCAGACGAGCGATAAACATGGTTATGTAGCCTGGCGCAAAGCCGCAGGCCAGCGTCCCGGCCGAGAAGAGAACGAt                  >  1:525180/1‑150 (MQ=255)
                                                                                                                                   cAGATGTTTTGGCCAGCTTTCAATGACATAGGTGGCGCTGGAACCGTATTCACCCGCCATCCCCATGCCGATGACCAGACGAGCGATAAACATGGTGATGTAGCCTGGCGCAAAGCCGCAGGCCAGCGTCCCGGCCGAGAAGAGAACGAt                  <  2:1877176/150‑1 (MQ=255)
                                                                                                                                     gATGTTTTGGCCAGCTTTCAATGACATAGGTGGCGCTGGAACCGTATTCACCCGCCATCCCCATGCCGATGACCAGACGAGCGATAAACATGGTGATGTAGCCTGGCGCAAAGCCGCAGGCCAGCGTCCCGGCCGAGAAGAGAACGATGc                <  1:659005/150‑1 (MQ=255)
                                                                                                                                      aTGTTTTGGCCAGCTTTCAATGACCTAGGTGGCGCTGGAACCGTATTCACCCGCCATCCCCATGCCGATGACCAGACGAGCGATAAACATGGTTATGTAGCCTGGCGCAAAGCCGCAGGCCAGCGTCCCGGCCGAGAAGAGAACGATGCt               <  2:3723721/150‑1 (MQ=255)
                                                                                                                                      aTGTTTTGGCCAGCTTTCAATGACATAGGTGGCGCTGGAACCGTATTCACCCGCCATCCCCATGCCGATGACCAGACGAGCGATAAACATGGTTATGTAGCCTGGCGCAAAGCCGCAGGCCAGCGTCCCGGCCGAGAAGAGAACGATGCt               <  2:3721929/150‑1 (MQ=255)
                                                                                                                                            tGGCCAGCTTTCAATGACATAGGTGGCGCTGGAACCGTATTCACCCGCCATCCCCATGCCGATGACCAGACGAGCGATAAACATGGTTATGTAGCCTGGCGCAAAGCCGCAGGCCAGCGTCCCGGCCGAGAAGAGAACGATGCTGGTGAc         >  2:816825/1‑150 (MQ=255)
                                                                                                                                            tGGCCAGCTTTCAATGACATAGGTGGCGCTGGAACCGTATTCACCCGCCATCCCCATGCCGATGACCAGACGAGCGATAAACATGGTGATGTAGCCTGGCGCAAAGCCGCAGGCCAGCGTCCCGGCCGAGAAGAGAACGATGCTGGTGAc         >  1:2649437/1‑150 (MQ=255)
                                                                                                                                             ggCCAGCTTTCAATGACATAGGTGGCGCTGGAACCGTATTCACCCGCCATCCCCATGCCGATGACCAGACGAGCGATAAACATGGTGATGTAGCCTGGCGCAAAGCCGCAGGCCAGCGTCCCGGCCGAGAAGAGAACGATGCTGGTGAcc        <  2:1192695/150‑1 (MQ=255)
                                                                                                                                               ccAGCTTTCAATGACATAGGTGGCGCTGGAACCGTATTCACCCGCCATCCCCATGCCGATGACCAGACGAGCGATAAACATGGTTATGTAGCCTGGCGCAAAGCCGCAGGCCAGCGTCCCGGCCGAGAAGAGAACGATGCTGGTGACCAt      >  1:1036086/1‑150 (MQ=255)
                                                                                                                                                 aGCTTTCAATGACATAGGTGGCGCTGGAACCGTATTCACCCGCCATCCCCATGCCGATGACCAGACGAGCGATAAACATGGTTATGTAGCCTGGCGCAAAGCCGCAGGCCAGCGTCCCGGCCGAGAAGAGAACGATGCTGGTGACCATTg    >  2:4522784/1‑150 (MQ=255)
                                                                                                                                                   cTTTCAATGACATAGGTGGCGCTGGAACCGTATTCACCCGCCATCCCCATGCCGATGACCAGACGAGCGATAAACATGGTTATGTAGCCTGGCGCAAAGCCGCAGGCCAGCGTCCCGGCCGAGAAGAGAACGATGCTGGTGACCATTGcc  >  1:4512237/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                                     
ATGATTGGCAAAATGCCGATAAAGAACAGCGCACGCCAGCCCCAGACCGGAACCACCAGGCTATAGACCTGAGCGGCAACGACGGCCCCCACAGAGAAGCCTGAAATCAAAAAACCACTGGCTTTGTTACGCAGATGTTTTGGCCAGCTTTCAATGACATAGGTGGCGCTGGAACCGTATTCACCCGCCATCCCCATGCCGATGACCAGACGAGCGATAAACATGGTGATGTAGCCTGGCGCAAAGCCGCAGGCCAGCGTCCCGGCCGAGAAGAGAACGATGCTGGTGACCATTGCC  >  CP009273/3365380‑3365676

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑