Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,400,740 A→T 5.9% T3S (ACT→TCT)  yhdT → DUF997 family putative inner membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,400,7400AT 5.9% 1129.4 / 42.4 336T3S (ACT→TCT) yhdTDUF997 family putative inner membrane protein
Reads supporting (aligned to +/- strand):  ref base A (180/136);  new base T (12/8);  total (192/144)
Fisher's exact test for biased strand distribution p-value = 8.21e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 346 total aligned reads displayed.

ATCTGGAGAAAAAACTCGGTCTTCAGGAAAAATAAGACTGCTAAAGCGTCAAAAGGCCGGATTTTCCGGCCTTTTTTATTACTGGGGATCGACAACCCCCATAAGGTACAATCCCCGCTTTCTTCACCCATCAGGGACAAAAAATGGACACTCGTTTTGTTCAGGCCCATAAAGAGGCGCGCTGGGCGCTGGGGCTGACCCTTTTGTATCTGGCAGTTTGGTTAGTAGCCGCTTACTTATCTGGCGTTGCCCCCGGTTTTACCGGCTTTCCGCGCTGGTTTGAGATGGCCTGCATCCTG  >  CP009273/3400591‑3400889
                                                                                                                                                     |                                                                                                                                                     
   tGGAGAAAAAACTCTGTCTTCAGGAAAAATAAGACTGCTAAAGCGTCAAAAGGCCGGATTTTCCGGCCTTTTTTATTACTGGGGATCGACAACCCCCATAAGGTACAATCCCCGCTTTCTTCACCCATCAGGGACAAAAAATGGACACTc                                                                                                                                                    <  1:3231792/150‑1 (MQ=255)
   tGGAGAAAAAACTCGGTCTTCAGGAAAAATAAGACTGCTAAAGCGTCAAAAGGCCGGATTTTCCGGCCTTTTTTATTACTGGGGATCGACAACCCCCATAAGGTACAATCCCCGCTTTCTTCACCCATCAGGGACAAAAAATGGACACTc                                                                                                                                                    <  1:956670/150‑1 (MQ=255)
    ggAGAAAAAACTCGGTCTTCAGGAAAAATAAGACTGCTAAAGCGTCAAAAGGCCGGATTTTCCGGCCTTTTTTATTACTGGGGATCGACAACCCCCATAAGGTACAATCCCCGCTTTCTTCACCCATCAGGGACAAAAAATGGACACTCg                                                                                                                                                   >  2:3207677/1‑150 (MQ=255)
        aaaaaaCTCGGTCTTCAGTAAAAATAAGACTGCTAAAGCGTCAAAAGGCCGGATTTTCCGGCCTTTTTTATTACTGGGGATCGACAACCCCCATAAGGTACAATCCCCGCTTTCTTCACCCATCAGGGACAAAAAATGGACACTCGtttt                                                                                                                                               <  1:459957/150‑1 (MQ=255)
          aaaaCTCGGTCTTCAGGAAAAATAAGACTGCTAAAGCGTCAAAAGGCCGGATTTTCCGGCCTTTTTTATTACTGGGGATCGACAACCCCCATAAGGTACAATCCCCGCTTTCTTCACCCATCAGGGACAAAAAATGGACACTCGTTTTGt                                                                                                                                             <  1:368361/150‑1 (MQ=255)
           aaaCTCGGTCTTCAGGAAAAATAAGACTGCTAAAGCGTCAAAAGGCCGGATTTTCCGGCCTTTTTTATTACTGGGGATCGACAACCCCCATAAGGTACAATCCCCGCTTTCTTCACCCATCAGGGACAAAAAATGGACACTCGTTTTGtt                                                                                                                                            <  2:1190671/150‑1 (MQ=255)
            aaCTCTGTCTTCAGGAAAAATAAGACTGCTAAAGCGTCAAAAGGCCGGATTTTCCGGCCTTTTTTATTACTGGGGATCGACAACCCCCATAAGGTACAATCCCCGCTTTCTTCACCCATCAGGGACAAAAAATGGACTCTCGTtttttta                                                                                                                                           >  1:3025945/1‑149 (MQ=255)
            aaCTCGGTCTTCAGGAAAAATAAGACTGCTAAAGCGTCAAAAGGCCGGATTTTCCGGCCTTTTTTATTACTGGGGATCGACAACCCCCATAAGGTACAATCCCCGCTTTCTTCACCCATCAGGGACAAAAAATGGACACTCGTTTTGTTc                                                                                                                                           <  2:2811789/150‑1 (MQ=255)
             aCTCGGTCTTCAGGAAAAATAAGACTGCTAAAGCGTCAAAAGGCCGGATTTTCCGGCCTTTTTTATTACTGGGGATCGACAACCCCCATAAGGTACAATCCCCGCTTTCTTCACCCATCAGGGACAAAAAATGGACTCTCGTTTTGTTCa                                                                                                                                          >  2:4294316/1‑150 (MQ=255)
                  gTCTTCAGGAAAAATAAGACTGCTAAAGCGTCAAAAGGCCGGATTTTCCGGCCTTTTTTATTACTGGGGATCGACAACCCCCATAAGGTACAATCCCCGCTTTCTTCACCCATCAGGGACAAAAAATGGACTCTCGTTTTGTTCAGGccc                                                                                                                                     >  2:603927/1‑150 (MQ=255)
                   tCTTCAGGAAAAATAAGACTGCTAAAGCGTCAAAAGGCCGGATTTTCCGGCCTTTTTTATTACTGGGGATCGACAACCCCCATAAGGTACAATCCCCGCTTTCTTCACCCATCAGGGACAAAAAATGGACACTCGTTTTGTTCAGGCCCa                                                                                                                                    <  2:1736371/150‑1 (MQ=255)
                       cAGGAAAAATAAGACTGCTAAAGCGTCAAAAGGCCGGATTTTCCGGCCTTTTTTATTACTGGGGATCGACAACCCCCATAAGGTACAATCCCCGCTTTCTTCACCCATCAGGGACAAAAAATGGACACTCGTTTTGTTCAGGCCCATaaa                                                                                                                                >  2:3786528/1‑150 (MQ=255)
                       cAGGAAAAATAAGACTGCTAAAGCGTCAAAAGGCCGGATTTTCCGGCCTTTTTTATTACTGGGGATCGACAACCCCCATAAGGTACAATCCCCGCTTTCTTCACCCATCAGGGACAAAAAATGGACACTCGTTTTGTTCAGGCCCATaaa                                                                                                                                >  1:238267/1‑150 (MQ=255)
                        aGGAAAAATAAGACTGCTAAAGCGTCAAAAGGCCGGATTTTCCGGCCTTTTTTATTACTGGGGATCGACAACCCCCATAAGGTACAATCCCCGCTTTCTTCACCCATCAGGGACAAAAAATGGCCACTCGTTTTGTTCAGGCCCATAAag                                                                                                                               >  2:2394763/1‑150 (MQ=255)
                         ggAAAAATAAGACTGCTAAAGCGTCAAAAGGCCGGATTTTCCGGCCTTTTTTATTACTGGGGATCGACAACCCCCATAAGGTACAATCCCCGCTTTCTTCACCCATCAGGGACAAAAAATGGACACTCGTTTTGTTCAGGCCCATAAaga                                                                                                                              >  1:3281468/1‑150 (MQ=255)
                         ggAAAAATAAGACTGCTAAAGCGTCAAAAGGCCGGATTTTCCGGCCTTTTTTATTACTGGGGATCGACAACCCCCATAAGGTACAATCCCCGCTTTCTTCACCCATCAGGGACAAAAAATGGACACTCGTTTTGTTCAGGCCCATAAaga                                                                                                                              >  1:2909653/1‑150 (MQ=255)
                            aaaaTAAGACTGCTAAAGCGTCAAAAGGCCGGATTTTCCGGCCTTTTTTATTACTGGGGATCGACAACCCCCATAAGGTACAATCCCCGCTTTCTTCACCCATCAGGGACAAAAAATGGACACTCGTTTTGTTCAGGCCCATAAAGAGgc                                                                                                                           >  2:4177578/1‑150 (MQ=255)
                             aaaTAAGACTGCTAAAGCGTCAAAAGGCCGGATTTTCCGGCCTTTTTTATTACTGGGGATCGACAACCCCCATAAGGTACAATCCCCGCTTTCTTCACCCATCAGGGACAAAAAATTGAAACTCGTTTTGTTCAGGCCCATAAAGAGgcg                                                                                                                          >  1:2948910/1‑150 (MQ=255)
                              aaTAAGACTGCTAAAGCGTCAAAAGGCCGGATTTTCCGGCCTTTTTTATTACTGGGGATCGACAACCCCCATAAGGTACAATCCCCGCTTTCTTCACCCATCAGGGACAAAAAATGGACACTCGTTTTGTTCAGGCCCATAAAGAGgcgc                                                                                                                         >  1:2150718/1‑150 (MQ=255)
                              aaTAAGACTGCTAAAGCGTCAAAAGGCCGGATTTTCCGGCCTTTTTTATTACTGGGGATCGACAACCCCCATAAGGTACAATCCCCGCTTTCTTCACCCATCAGGGACAAAAAATGGACACTCGTTTTGTTCAGGCCCATAAAGAGgcgc                                                                                                                         >  2:1333215/1‑150 (MQ=255)
                               aTAAGACTGCTAAAGCGTCAAAAGGCCGGATTTTCCGGCCTTTTTTATTACTGGGGATCGACAACCCCCATAAGGTACAATCCCCGCTTTCTTCACCCATCAGGGACAAAAAATGGACACTCGTTTTGTTCAGGCCCATAAAGAGgcgcg                                                                                                                        >  1:485262/1‑150 (MQ=255)
                                tAAGACTGCTAAAGCGTCAAAAGGCCGGATTTTCCGGCCTTTTTTATTACTGGGGATCGACAACCCCCATAAGGTACAATCCCCGCTTTCTTCACCCATCAGGGACAAAAAATGGACTCTCGTTTTGTTCAGGCCCATAAAGAGgcgcgc                                                                                                                       >  1:3503019/1‑150 (MQ=255)
                                 aaGCCTGCTAAAGCGTCAAAAGGCCGGATTGTCCGGCCTTTTTTATTACTGGGGATCGACAACCCCCATAAGGTACAATCCCCGCTTTCTTCACCCATCAGGGACAAAAACTGGACACTCGTTTTGTTCAGGCCCATAAAGAGGCgcgct                                                                                                                      <  2:1812887/150‑1 (MQ=255)
                                 aaGACTGCTAAAGCGTCAAAAGGCCGGATTTTCCGGCCTTTTTTATTACTGGGGATCGACAACCCCCATAAGGTACAATCCCCGCTTTCTTCACCCATCAGGGACAAAAAATGGACACTCGTTTTGTTCAGGCCCATAAAGAGGCgcgct                                                                                                                      <  2:2078714/150‑1 (MQ=255)
                                   gACTGCTAAAGCGTCAAAAGGCCGGATTTTCCGGCCTTTTTTATTACTGGGGCTCGACAACCCCCATAAGGTCCAATCCCCGCTTTCTTCACCCATCAGGGACAAAAAATGGACACTCGTTTTGTTCAGGCCCATAAAGAGGCgcgctgg                                                                                                                    >  2:1198976/1‑150 (MQ=255)
                                   gACTGCTAAAGCGTCAAAAGGCCGGATTTTCCGGCCTTTTTTATTACTGGGGATCGACAACCCCCATAAGGTACAATCCCCGCTTTCTTCACCCATCAGGGACAAAAAATGGACACTCGTTTTGTTCAGGCCCATAAAGAGGCgcgctgg                                                                                                                    >  2:709965/1‑150 (MQ=255)
                                        cTAAAGCGTCAAAAGGCCGGATTTTCCGGCCTTTTTTATTACTGGGGATCGACAACCCCCATAAGGTACAATCCCCGCTTTCTTCACCCATCAGGGACAAAAAATGGACACTCGTTTTGTTCAGGCCCATAAAGAGGCgcgctgggcgct                                                                                                               >  2:4467676/1‑150 (MQ=255)
                                        cTAAAGCGTCAAAAGGCCGGATTTTCCGGCCTTTTTTATTACTGGGGATCGACAACCCCCATAAGGTACAATCCCCGCTTTCTTCACCCATCAGGGACAAAAAATGGACACTCGTTTTGTTCAGGCCCATAAAGAGGCgcgctgggcgct                                                                                                               <  1:2534893/150‑1 (MQ=255)
                                        cTAAAGCGTCAAAAGGCCGGATTTTCCGGCCTTTTTTATTACTGGGGATCGACAACCCCCATAAGGTACAATCCCCGCTTTCTTCACCCATCAGGGACAAAAAATGGACACTCGTTTTGTTCAGGCCCATAAAGAGGCgcgctgggcgct                                                                                                               <  1:3197513/150‑1 (MQ=255)
                                          aaaGCGTCAAAAGGCCGGATTTTCCGGCCTTTTTTATTACTGGGGATCGACAACCCCCATAAGGTACAATCCCCGCTTTCTTCACCCATCAGGGACAAAAAATGGACACTCGTTTTGTTCAGGCCCATAAAGAGGCgcgctgggcgctgg                                                                                                             >  2:650618/1‑150 (MQ=255)
                                           aaGCGTCAAAAGGCCGGATTTTCCGGCCTTTTTTATTACTGGGGATCGACAACCCCCATAAGGTACAATCCCCGCTTTCTTCACCCATCAGGGACAAAAAATGGACACTCGTTTTGTTCAGGCCCATAAAGAGGCGcgctgggcgctggg                                                                                                            >  2:533900/1‑150 (MQ=255)
                                            aGCGTCAAAAGGCCGGATTTTCCGGCCTTTTTTATTACGGGGGATCCACAACCCCCATAAGGTACAATCCCCGCTTTCTTCACCCATCAGGGACAAAAAATGGACACTCGTTTTGTTCAGGCCCCTAAAGAGGCGCGCTGGGCGCTgggg                                                                                                           >  2:504060/1‑150 (MQ=255)
                                              cGTCAAAAGGCCGGATTTTCCGGCCTTTTTTATTACTGGGGATCGACAACCCCCATAAGGTACAATCCCCGCTTTCTTCACCCATCAGGGACAAAAAATGGACACTCGTTTTGTTCAGGCCCATAAAGAGGCGCGCTGGGCGCTGGGGCt                                                                                                         <  1:3043896/150‑1 (MQ=255)
                                               gTCAAAAGGCCGGATTTTCCGGCCTTTTTTATTACTGGGGATCGACAACCCCCATAAGGTACAATCCCCGCTTTCTTCACCCATCAGGGACAAAAAATGGACACTCGTTTTGTTCAGGCCCATAAAGAGGCGCGCTGGGCGCTGGGGCTg                                                                                                        >  2:1582618/1‑150 (MQ=255)
                                                  aaaaGGCCGGATTTTCCGGCCTTTTTTATTACTGGGGATCGACAACCCCCATAAGGTACAATCCCCGCTTTCTTCACCCATCAGGGACAAAAAATGGACACTCGTTTTGTTCAGGCCCATAAAGAGGCGCGCTGGGCGCTGGGGCTGAcc                                                                                                     >  2:471822/1‑150 (MQ=255)
                                                  aaaaGGCCGGATTTTCCGGCCTTTTTTATTACTGGGGATCGACAACCCCCATAAGGTACAATCCCCGCTTTCTTCACCCATCAGGGACAAAAAATGGACACTCGTTTTGTTCAGGCCCATAAAGAGGCGCGCTGGGCGCTGGGGCTGAcc                                                                                                     >  1:4501898/1‑150 (MQ=255)
                                                    aaGGCCGGATTTTCCGGCCTTTTTTATTACTGGGGATCGACAACCCCCATAAGGTACAATCCCCGCTTTCTTCACCCATCAGGGACAAAAAATGGACACTCGTTTTGTTCAGGCCCATAAAGAGGCGCGCTGGGCGCTGGGGCTGACCCt                                                                                                   >  2:3144545/1‑150 (MQ=255)
                                                        ccGGATTTTCCGGCCTTTTTTATTACTGGGGATCGACAACCCCCATAAGGTACAATCCCCGCTTTCTTCACCCATCAGGGACAAAAAATGGACACTCGTTTTGTTCAGGCCCATAAAGAGGCGCGCTGGGCGCTGGGGCTGACCCTTTTg                                                                                               <  2:360790/150‑1 (MQ=255)
                                                        ccGGATTTTCCGGCCTTTTTTATTACTGGGGATCGACAACCCCCATAAGGTACAATCCCCGCTTTCTTCACCCATCAGGGACAAAAAATGGACACTCGTTTTGTTCAGGCCCATAAAGAGGCGCGCTGGGCGCTGGGGCTGACCCTTTTg                                                                                               >  1:2578731/1‑150 (MQ=255)
                                                        ccGGATTTTCCGGCCTTTTTTATTACTGGGGATCGACAACCCCCATAAGGTACAATCCCCGCTTTCTTCACCCATCAGGGACAAAAAATGGACACTCGTTTTGTTCAGGCCCATAAAGAGGCGCGCTGGGCGCTGGGGCTGACCCTTTTg                                                                                               >  1:1865194/1‑150 (MQ=255)
                                                        ccGGATTTTCCGGCCTTTTTTATTACTGGGGATCGACAACCCCCATAAGGTACAATCCCCGCTTTCTTCACCCATCAGGGACAAAAAATGGACACTCGTTTTGTTCAGGCCCATAAAGAGGCGCGCTGGGCGCTGGGGCTGACCCTTTTg                                                                                               <  1:3960013/150‑1 (MQ=255)
                                                           gATTTTCCGGCCTTTTTTATTACTGGGGATCGACAACCCCCATAAGGTACAATCCCCGCTTTCTTCACCCATCAGGGACAAAAAATGGACTCTCGTTTTGTTCAGGCCCATAAAGAGGCGCGCTGGGCGCTGGGGCTGACCCTTTTGTAt                                                                                            >  2:1068648/1‑150 (MQ=255)
                                                           gATGTTCCGGCCTTTTTTATTACTGGGGATCGACAACCCCCATAAGGTACAATCCCCGCTTTCTTCACCCCTCAGGGACAAAAAATGGACACTCGTTTTGTTCAGGCCCATAAAGAGGCGCGCTGGGCGCTGGGGCTGACCCTTTTGTAt                                                                                            <  1:2464140/150‑1 (MQ=255)
                                                                 ccGGCCTTTTTTATTACTGGGGATCGACAACCCCCATAAGGTACAATCCCCGCTTTCTTCACCCATCAGGGACAAAAAATGGACACTCGTTTTGTTCAGGCCCATAAAGAGGCGCGCTGGGCGCTGGGGCTGACCCTTTTGTATCTGGCa                                                                                      >  2:2451917/1‑150 (MQ=255)
                                                                  cGGCCTTTTTTATTACTGGGGATCGACAACCCCCATAAGGTACAATCCCCGCTTTCTTCACCCATCAGGGACAAAAAATGGACACTCGTTTTGTTCAGGCCCATAAAGAGGCGCGCTGGGCGCTGGGGCTGACCCTTTTGTATCTGGCAg                                                                                     <  1:1922258/150‑1 (MQ=255)
                                                                    gCCTTTTTTATTACTGGGGATCGACAACCCCCATAAGGTACAATCCCCGCTTTCTTCACCCATCAGGGACAAAAAATGGACACTCGTTTTGTTCAGGCCCATAAAGAGGCGCGCTGGGCGCTGGGGCTGACCCTTTTGTATCTGGCAGtt                                                                                   >  2:2685270/1‑150 (MQ=255)
                                                                     ccTTTTTTATTACTGGGGATCGACAACCCCCATAAGGTACAATCCCCGCTTTCTTCACCCATCAGGGACAAAAAATGGACACTCGTTTTGTTCAGGCCCATAAAGAGGCGCGCTGGGCGCTGGGGCTGACCCTTTTGTATCTGGCAGttt                                                                                  <  2:3341240/150‑1 (MQ=255)
                                                                     ccTTTTTTATTACTGGGGATCGACAACCCCCATAAGGTACAATCCCCGCTTTCTTCACCCATCAGGGACAAAAAATGGACACTCGTTTTGTTCAGGCCCATAAAGAGGCGCGCTGGGCGCTGGGGCTGACCCTTTTGTATCTGGCAGttt                                                                                  >  2:3551771/1‑150 (MQ=255)
                                                                        tttttATTACTGGGGATCGACAACCCCCATAAGGTACAATCCCCGCTTTCTTCACCCATCAGGGACAAAAAATGGACACTCGTTTTGTTCAGGCCCATAAAGAGGCGCGCTGGGCGCTGGGGCTGACCCTTTTGTATCTGGCAGTTTGGt                                                                               >  2:1696644/1‑150 (MQ=255)
                                                                        tttttATTACTGGGGATCGACAACCCCCATAAGGTACAATCCCCGCTTTCTTCACCCATCAGGGACAAAAAATGGACACTCGTTTTGTTCAGGCCCATAAAGAGGCGCGCTGGGCGCTGGGGCTGACCCTTTTGTATCTGGCAGTTTGGt                                                                               >  2:3975063/1‑150 (MQ=255)
                                                                          tttATTACTGGGGATCGACAACCCCCATAAGGTACAATCCCCGCTTTCTTCACCCATCAGGGACAAAAAATGGACACTCGTTTTGTTCAGGCCCATAAAGAGGCGCGCTGGGCGCTGGGGCTGACCCTTTTGTATCTGGCAGTTTGGTta                                                                             >  1:3366886/1‑150 (MQ=255)
                                                                          tttATTACTGGGGATCGACAACCCCCATAAGGTACAATCCCCGCTTTCTTCACCCATCAGGGACAAAAAATGGACACTCGTTTTGTTCAGGCCCATAAAGAGGCGCGCTGGGCGCTGGGGCTGACCCTTTTGTATCTGGCAGTTTGGTta                                                                             <  2:485262/150‑1 (MQ=255)
                                                                                   ggggATCGACAACCCCCATAAGGTACAATCCCCGCTTTCTTCACCCATCAGGGACAAAAAATGGACACTCGTTTTGTTCAGGCCCATAAAGAGGCGCGCTGGGCGCTGGGGCTGACCCTTTTGTATCTGGCAGTTTGGTTAGTAGCCGct                                                                    >  1:3757731/1‑150 (MQ=255)
                                                                                      gATCGACAACCCCCATAAGGTACAATCCCCGCTTTCTTCACCCATCAGGGACAAAAAATGGACACTCGTTTTGTTCAGGCCCATAAAGAGGCGCGCTGGGCGCTGGGGCTGACCCTTTTGTATCTGGCAGTTTGGTTAGTAGCCGcttac                                                                 >  1:100296/1‑150 (MQ=255)
                                                                                      gATCGACAACCCCCATAAGGTACAATCCCCGCTTTCTTCACCCATCAGGGACAAAAAATGGACACTCGTTTTGTTCAGGCCCATAAAGAGGCGCGCTGGGCGCTGGGGCTGACCCTTTTGTATCTGGCAGTTTGGTTAGTAGCCGcttac                                                                 <  1:343152/150‑1 (MQ=255)
                                                                                      gATCGACAACCCCCATAAGGTACAATCCCCGCTTTCTTCACCCATCAGGGACAAAAAATGGACACTCGTTTTGTTCAGGCCCATAAAGAGGCGCGCTGGGCGCTGGGGCTGACCCTTTTGTATCTGGCAGTTTGGTTAGTAGCCGcttac                                                                 >  1:2250119/1‑150 (MQ=255)
                                                                                      gATCGACAACCCCCATAAGGTACAATCCCCGCTTTCTTCACCCATCAGGGACAAAAAATGGACACTCGTTTTGTTCAGGCCCATAAAGAGGCGCGCTGGGCGCTGGGGCTGACCCTTTTGTATCTGGCAGTTTGGTTAGTAGCCGcttac                                                                 >  1:4524225/1‑150 (MQ=255)
                                                                                      gATCGACAACCCCCATAAGGTACAATCCCCGCTTTCTTCACCCATCAGGGACAAAAAATGGACACTCGTTTTGTTCAGGCCCATAAAGAGGCGCGCTGGGCGCTGGGGCTGACCCTTTTGTATCTGGCAGTTTGGTTAGTAGCCGcttac                                                                 >  2:1939448/1‑150 (MQ=255)
                                                                                           aCAACCCCCATAAGGTACAATCCCCGCTTTCTTCACCCATCAGGGACAAAAAATGGACACTCGTTTTGTTCAGGCCCATAAAGAGGCGCGCTGGGCGCTGGGGCTGACCCTTTTGTATCTGGCAGTTTGGTTAGTAGCCGCTTACTTATc                                                            <  1:3953981/150‑1 (MQ=255)
                                                                                            cAACCCCCATAAGGTACAATCCCCGCTTTCTTCACCCATCAGGGACAAAAAATGGACACTCGTTTTGTTCAGGCCCATAAAGAGGCGCGCTGGGCGCTGGGGCTGACCCTTTTGTATCTGGCAGTTTGGTTAGTAGCCGCTTACTTATCt                                                           <  1:2905435/150‑1 (MQ=255)
                                                                                            cAACCCCCATAAGGTACAATCCCCGCTTTCTTCACCCATCAGGGACAAAAAATGGACACTCGTTTTGTTCAGGCCCATAAAGAGGCGCGCTGGGCGCTGGGGCTGACCCTTTTGTATCTGGCAGTTTGGTTAGTAGCCGCTTACTTATCt                                                           <  1:2322397/150‑1 (MQ=255)
                                                                                             aaCCCCCATAAGGTACAATCCCCGCTTTCTTCACCCATCAGGGACAAAAAATGGACACTCGTTTTGTTCAGGCCCATAAAGAGGCGCGCGGGGCGCTGGGGCTGACCCTTTTGTATCTGGCAGTTTGGTTAGTAGCCGCTTACTTATCTg                                                          <  1:2819220/150‑1 (MQ=255)
                                                                                               cccccATAAGGTACAATCCCCGCTTTCTTCACCCATCAGGGACAAAAAATGGACACTCGTTTTGTTCAGGCCCATAAAGAGGCGCGCGGGGCGCTGGGGCTGACCCTTTTGTATCTGGCAGTTTGGTTAGTAGCCGCTTACTTATCTGGc                                                        <  2:1897415/150‑1 (MQ=255)
                                                                                                ccccATAAGGTACAATCCCCGCTTTCTTCACCCATCAGGGACAAAAAATGGACACTCGTTTTGTTCAGGCCCATAAAGAGGCGCGCTGGGCGCTGGGGCTGACCCTTTTGTATCTGGCAGTTTGGTTAGTAGCCGCTTACTTATCTGGCg                                                       <  1:119063/150‑1 (MQ=255)
                                                                                                ccccATAAGGTACAATCCCCGCTTTCTTCACCCATCAGGGACAAAAAATGGACACTCGTTTTGTTCAGGCCCATAAAGAGGCGCGCTGGGCGCTGGGGCTGACCCTTTTGTATCTGGCAGTTTGGTTAGTAGCCGCTTACTTATCTGGCg                                                       <  1:389765/150‑1 (MQ=255)
                                                                                                  ccATAAGGTACAATCCCCGCTTTCTTCACCCATCAGGGACAAAAAATGGACACTCGTTTTGTTCAGGCCCATAAAGAGGCGCGCTGGGCGCTGGGGCTGACCCTTTTGTATCTGGCAGTTTGGTTAGTAGCCGCTTACTTATCTGGCGtt                                                     <  1:2616409/150‑1 (MQ=255)
                                                                                                    aTAAGGTACAATCCCCGCTTTCTTCACCCATCAGGGACAAAAAATGGACACTCGTTTTGTTCAGGCCCATAAAGAGGCGCGCTGGGCGCTGGGGCTGACCCTTTTGTATCTGGCAGTTTGGTTAGTAGCCGCTTACTTATCTGGCGTTGc                                                   >  2:2155708/1‑150 (MQ=255)
                                                                                                      aaGGTACAATCCCCGCTTTCTTCACCCATCAGGGACAAAAAATGGACACTCGTTTTGTTCAGGCCCATAAAGAGGCGCGCTGGGCGCTGGGGCTGACCCTTTTGTATCTGGCAGTTTGGTTAGTAGCCGCTTACTTATCTGGCGTTGccc                                                 >  2:2934867/1‑150 (MQ=255)
                                                                                                      aaGGTACAATCCCCGCTTTCTTCACCCATCAGGGACAAAAAATGGACACTCGTTTTGTTCAGGCCCATAAAGAGGCGCGCTGGGCGCTGGGGCTGACCCTTTTGTATCTGGCAGTTTGGTTAGTAGCCGCTTACTTATCTGGCGTTGccc                                                 >  1:574018/1‑150 (MQ=255)
                                                                                                      aaGGTACAATCCCCGCTTTCTTCACCCATCAGGGACAAAAAATGGACACTCGTTTTGTTCAGGCCCATAAAGAGGCGCGCTGGGCGCTGGGGCTGACCCTTTTGTATCTGGCAGTTTGGTTAGTAGCCGCTTACTTATCTGGCGTTGccc                                                 >  1:717915/1‑150 (MQ=255)
                                                                                                        ggTACAATCCCCGCTTTCTTCACCCATCAGGGACAAAAAATGGACACTCGTTTTGTTCAGGCCCATAAAGAGGCGCGCTGGGCGCTGGGGCTGACCCTTTTGTATCTGGCAGTTTGGTTAGTAGCCGCTTACTTATCTGGCGTTGccccc                                               <  2:1286997/150‑1 (MQ=255)
                                                                                                           aCAATCCCCGCTTTCTTCACCCATCAGGGACAAAAAATGGACACTCGTTTTGTTCAGGCCCATAAAGAGGCGCGCTGGGCGCTGGGGCTGACCCTTTTGTATCTGGCAGTTTGGTTAGTAGCCGCTTACTTATCTGGCGTTGCCCCCGGt                                            >  1:1543681/1‑150 (MQ=255)
                                                                                                           aCAATCCCCGCTTTCTTCACCCATCAGGGACAAAAAATGGACACTCGTTTTGTTCAGGCCCATAAAGAGGCGCGCTGGGCGCTGGGGCTGACCCTTTTGTATCTGGCAGTTTGGTTAGTAGCCGCTTACTTATCTGGCGTTGCCCCCGGt                                            >  1:2048194/1‑150 (MQ=255)
                                                                                                            cAATCCCCGCTTTCTTCACCCATCAGGGACAAAAAATGGACACTCGTTTTGTTCAGGCCCATAAAGAGGCGCGCTGGGCGCTGGGGCTGACCCTTTTGTATCTGGCAGTTTGGTTAGTAGCCGCTTACTTATCTGGCGTTGCCCCCGGtt                                           >  1:2101498/1‑150 (MQ=255)
                                                                                                            cAATCCCCGCTTTCTTCACCCATCAGGGACAAAAAATGGACACTCGTTTTGTTCAGGCCCATAAAGAGGCGCGCGGGGCGCTGGGGCTGACCCTTTTGTATCTGGCAGTTTGGTTAGTAGCCGCTTACTTATCTGGCGTTGCCCCCGGtt                                           <  1:3846387/150‑1 (MQ=255)
                                                                                                                ccccGCTTTCTTCACCCATCAGGGACAAAAAATGGACACTCGTTTTGTTCAGGCCCATAAAGCGGCGCGCTGGGCGCTGGGGCTGACCCTTTTGTATCTGGCAGTTTGGTTAGTAGCCGCTTACTTATCTGGCGTTGCCCCCGGTTTTAc                                       <  2:2246095/150‑1 (MQ=255)
                                                                                                                 cccGCTTTCTTCACCCATCAGGGACAAAAAATGGACACTCGTTTTGTTCAGGCCCATAAAGAGGCGCGCTGGGCGCTGGGGCTGACCCTTTTGTATCTGGCAGTTTGGTTAGTAGCCGCTTACTTATCTGGCGTTGCCCCCGGTTTTAcc                                      <  1:2193505/150‑1 (MQ=255)
                                                                                                                  ccGCTTTCTTCACCCATCAGGGACAAAAAATGGACACTCGTTTTGTTCAGGCCCATAAAGAGGCGCGCTGGGCGCTGGGGCTGACCCTTTTGTATCTGGCAGTTTGGTTAGTAGCCGCTTACTTATCTGGCGTTGCCCCCGGTTTTACCg                                     >  2:1760075/1‑150 (MQ=255)
                                                                                                                  ccGCTTTCTTCACCCATCAGGGACAAAAAATGGACACTCGTTTTGTTCAGGCCCATAAAGAGGCGCGCTGGGCGCTGGGGCTGACCCTTTTGTATCTGGCAGTTTGGTTAGTAGCCGCTTACTTATCTGGCGTTGCCCCCGGTTTTACCg                                     >  1:756276/1‑150 (MQ=255)
                                                                                                                       ttcttcACCCATCAGGGACAAAAAATGGACACTCGTTTTGTTCAGGCCCATAAAGAGGCGCGCTGGGCGCTGGGGCTGACCCTTTTGTATCTGGCAGTTTGGTTAGTAGCCGCTTACTTATCTGGCGTTGCCCCCGGTTTTACCGGCttt                                >  1:1262225/1‑150 (MQ=255)
                                                                                                                            cACCCATCAGGGACAAAAAATGGACACTCGTTTTGTTCAGGCCCATAAAGAGGCGCGCTGGGCGCTGGGGCTGACCCTTTTGTATCTGGCAGTTTGGTTAGTAGCCGCTTACTTATCTGGCGTTGCCCCCGGTTTTACCGGCTTTCcgcg                           >  2:1401979/1‑150 (MQ=255)
                                                                                                                            cACCCATCAGGGACAAAAAATGGACACTCGTTTTGTTCAGGCCCATAAAGAGGCGCGCTGGGCGCTGGGGCTGACCCTTTTGTATCTGGCAGTTTGGTTAGTAGCCGCTTACTTATCTGGCGTTGCCCCCGGTTTTACCGGCTTTCcgcg                           >  2:3685131/1‑150 (MQ=255)
                                                                                                                                 aTCAGGGACAAAAAATGGACACTCGTTTTGTTCAGGCCCATACAGAGGCGCGCTGGGCGCTGGGGCTGACCCTTTTGTATCTGGCAGTTTGGTTAGTAGCCGCTTACTTATCTGGCGTTGCCCCCGGTTTTACCGGCTTTCCGCGCTGGt                      >  2:85403/1‑150 (MQ=255)
                                                                                                                                 aTCAGGGACAAAAAATGGACACTCGTTTTGTTCAGGCCCATAAAGAGGCGCGCTGGGCGCTGGGGCTGACCCTTTTGTATCTGGCAGTTTGGTTAGTAGCCGCTTACTTATCTGGCGTTGCCCCCGGTTTTACCGGCTTTCCGCGCTGGt                      >  1:265284/1‑150 (MQ=255)
                                                                                                                                   cAGGGACAAAAACTGGACACTCGTTTTGTTCAGGCCCATAAAGAGGCGCGCTGGGCGCTGGGGCTGACCCTTTTGTATCTGGCAGTTTGGTTAGTAGCCGCTTACTTATCTGGCGTTGCCCCCGGTTTTACCGGCTTTCCGCGCTGGttt                    <  2:3260889/150‑1 (MQ=255)
                                                                                                                                   cAGGGACAAAAAATGGACACTCGTTTTGTTCAGGCCCATAAAGAGGCGCGCTGGGCGCTGGGGCTGACCCTTTTGTATCTGGCAGTTTGGTTAGTAGCCGCTTACTTATCTGGCGTTGCCCCCGGTTTTACCGGCTTTCCGCGCTGGttt                    <  2:4088956/150‑1 (MQ=255)
                                                                                                                                   cAGGGACAAAAAATGGACACTCGTTTTGTTCAGGCCCATAAAGAGGCGCGCTGGGCGCTGGGGCTGACCCTTTTGTATCTGGCAGTTTGGTTAGTAGCCGCTTACTTATCTGGCGTTGCCCCCGGTTTTACCGGCTTTCCGCGCTGGttt                    <  1:4048547/150‑1 (MQ=255)
                                                                                                                                      ggACAAAAAATGGACACTCGTTTTGTTCAGGCCCATAAAGAGGCGCGCTGGGCGCTGGGGCTGACCCTTTTGTATCTGGCAGTTTGGTTAGTAGCCGCTTACTTATCTGGCGTTGCCCCCGGTTTTACCGGCTTTCCGCGCGGGTTTgag                 >  2:3879274/1‑150 (MQ=255)
                                                                                                                                       gACAAAAAATGGACTCTCGTTTTGTTCAGGCCCATAAAGAGGCGCGCTGGGCGCTGGGGCTGACCCTTTTGTATCTGGCAGTTTGGTTAGTAGCCGCTTAATTATCTGGCGTTGCCCCCGGTTTTACCGGCTTTCCGCGCTGGTTTgaga                <  1:2232518/150‑1 (MQ=255)
                                                                                                                                        aCAAAAAATGGACACTCGTTTTGTTCAGGCCCATAAAGAGGCGCGCTGGGCGCTGGGGCTGACCCTTTTGTATCTGGCAGTTTGGTTAGTAGCCGCTTACTTATCTGGCGTTGCCCCCGGTTTTACCGGCTTTCCGCGCTGGTTTGAGAt               <  1:2685270/150‑1 (MQ=255)
                                                                                                                                          aaaaaaTGGACACTCGTTTTGTTCAGGCCCATAAAGAGGCGCGCTGGGCGCTGGGGCTGACCCTTTTGTATCTGGCAGTTTGGTTAGTAGCCGCTTACTTATCTGGCGTTGCCCCCGGTTTTACCGGCTTTCCGCGCTGGTTTGAGATgg             <  1:136144/150‑1 (MQ=255)
                                                                                                                                            aaaaTGGACACTCGTTTTGTTCAGGCCCATAAAGAGGCGCGCTGGGCGCTGGGGCTGACCCTTTTGTATCTGGCAGTTTGGTTAGTAGCCGCTTACTTATCTGGCGTTGCCCCCGGTTTTACCGGCTTTCCGCGCTGGTTTGAGATGGcc           >  1:3031683/1‑150 (MQ=255)
                                                                                                                                            aaaaTGGACACTCGTTTTGTTCAGGCCCATAAAGAGGCGCGCTGGGCGCTGGGGCTGACCCTTTTGTATCTGGCAGTTTGGTTAGTAGCCGCTTACTTATCTGGCGTTGCCCCCGGTTTTACCGGCTTTCCGCGCTGGTTTGAGATGGcc           >  2:516367/1‑150 (MQ=255)
                                                                                                                                            aaaaTGGACACTCGTTTTGTTCAGGCCCATAAAGAGGCGCGCTGGGCGCTGGGGCTGACCCTTTTGTATCTGGCAGTTTGGTTAGTAGCCGCTTACTTATCTGGCGTTACCCCCGGTTTTACCGGCTTTCCGCGCTGGTTTGAGATGGcc           >  1:3656456/1‑150 (MQ=255)
                                                                                                                                             aaaTGGACACTCGTTTTGTTCAGGCCCATAAAGAGGCGCGCTGGGCGCTGGGGCTGACCCTTTTGTATCTGGCAGTTTGGTTAGTAGCCGCTTACTTATCTGGCGTTGCCCCCGGTTTTACCGGCTTTCCGCGCTGGTTTGAGATGGCCt          >  1:3510426/1‑150 (MQ=255)
                                                                                                                                             aaaTGGACACTCGTTTTGTTCAGGCCCATAAAGAGGCGCGCTGGGCGCTGGGGCTGACCCTTTTGTATCTGGCAGTTTGGTTAGTAGCCGCTTACTTATCTGGCGTTGCCCCCGGTTTTACCGGCTTTCCGCGCTGGTTTGAGATGGCCt          <  2:2565703/150‑1 (MQ=255)
                                                                                                                                                tGGACACTCGTTTTGTTCAGGCCCATAAAGAGGCGCGCTGGGCGCTGGGGCTGACCCTTTTGTATCTGGCAGTTTGGTTAGTAGCCGCTTACTTATCTGGCGTTGCCCCCGGTTTTACCGGCTTTCCGCGCTGGTTTGAGATGGCCTGCa       >  1:4345168/1‑150 (MQ=255)
                                                                                                                                                    cacTCGTTTTGTTCAGGCCCATAAAGAGGCGCGCTGGGCGCTGGGGCTGACCCTTTTGTATCTGGCAGTTTGGTTAGTAGCCGCTTACTTATCTGGCGTTGCCCCCGGTTTTACCGGCTTTCCGCGCTGGTTTGAGATGGCCTGCATCCt   <  1:1582618/150‑1 (MQ=255)
                                                                                                                                                    cacTCGTTTTGTTCAGGCCCATAAAGAGGCGCGCTGGGCGCTGGGGCTGACCCTTTTGTATCTGGCAGTTTGGTTAGTAGCCGCTTACTTATCTGGCGTTGCCCCCGGTTTTACCGGCTTTCCGCGCTGGTTTGAGATGGCCTGCATCCt   <  2:3034977/150‑1 (MQ=255)
                                                                                                                                                     acTCGTTTTGTTCAGGCCCATAAAGAGGCGCGCTGGGCGCTGGGGCTGACCCTTTTGTATCTGGCAGTTTGGTTAGTAGCCGCTTACTTATCTGGCGTTGCCCCCGGTTTTACCGGCTTTCCGCGCTGGTTTGAGATGGCCTGCATCCTg  <  1:1751728/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
ATCTGGAGAAAAAACTCGGTCTTCAGGAAAAATAAGACTGCTAAAGCGTCAAAAGGCCGGATTTTCCGGCCTTTTTTATTACTGGGGATCGACAACCCCCATAAGGTACAATCCCCGCTTTCTTCACCCATCAGGGACAAAAAATGGACACTCGTTTTGTTCAGGCCCATAAAGAGGCGCGCTGGGCGCTGGGGCTGACCCTTTTGTATCTGGCAGTTTGGTTAGTAGCCGCTTACTTATCTGGCGTTGCCCCCGGTTTTACCGGCTTTCCGCGCTGGTTTGAGATGGCCTGCATCCTG  >  CP009273/3400591‑3400889

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑