Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,520,457 C→A 5.5% V210V (GTC→GTA)  yrfF → inner membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,520,4570CA 5.5% 987.0 / 25.3 256V210V (GTC→GTA) yrfFinner membrane protein
Reads supporting (aligned to +/- strand):  ref base C (142/100);  new base A (11/3);  total (153/103)
Fisher's exact test for biased strand distribution p-value = 1.69e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.93e-01

Only 100 of 270 total aligned reads displayed.

AGCTATGCCTTGCAACCTGTTCCGTCAACGCAGGCGTCGATTCGCGGGGAAGAGAGTGAGCAAATCGAGCTACTCAATATTCGCAAAGAAACGCATGAAGAATATGCGCTGAGTCGTCCGCGCGGGCTGCGTGAAGCGTTGCTGATCGTCGCCTCCTTCCTGATGTTCTTTTTCTGCCTGATTACCCCGGATGTATTTGTTCCGTGGCTGGCAGGCGGCGCGTTACTGCTGCTGGGCGCAGGTCTGTGGGGGCTATTCGCGCCCCCGGCAAAATCCTCCCTGCGGGAAATTCATTGTCT  >  CP009273/3520308‑3520606
                                                                                                                                                     |                                                                                                                                                     
 gCTATGCCTTGCAACCTGTTCCGTCAACGCAGGCGTCGATTCGCGGGGAAGAGAGTGAGCAAATCGAGCTACTCAATATTCGCAAAGAAACGCATGAAGAATATGCGCTGAGTCGTCCGCGCGGGCTGCGTGAAGCGTTGCTGAtcgtcg                                                                                                                                                      >  1:1330120/1‑150 (MQ=255)
   tATGCCTTGCAACCTGTTCCGTCAACGCAGGCGTCGATTCGCGGGGAAGAGAGTGAGCAAATCGAGCTACTCAATATTCGCAAAGAAACGCATGAAGAATATGCGCTGAGTCGTCCGCGCGGGCTGCGTGAAGCGTTGCTGATCGTAGcc                                                                                                                                                    >  1:2298961/1‑150 (MQ=255)
     tGCCTTGCAACCTGTTCCGTCAACGCAGGCGTCGATTCGCGGGGAAGAGAGTGAGCAAATCGAGCTACTCAATATTCGCAAAGAAACGCATGAAGAATATGCGCTGAGTCGTCCGCGCGGGCTGCGTGAAGCGTTGCTGATCGTCGcctc                                                                                                                                                  >  1:4298045/1‑150 (MQ=255)
      gCCTTGCAACCTGTTCCGTCAACGCAGGCGTCGATTCGCGGGGAAGAGAGTGAGCAAATCGAGCTACTCAATATTCGCAAAGAAACGCATGAAGAATATGCGCTGAGTCGTCCGCGCGGGCTGCGTGAAGCGTTGCTGATCGTCGcctcc                                                                                                                                                 <  2:1221161/150‑1 (MQ=255)
           gCAACCTGTTCCGTCAACGCAGGCGTCGATTCGCGGGGAAGAGAGTGAGCAAATCGAGCTACTCAATATTCGCAAAGAAACGCATGAAGAATATGCGCTGAGTCGTCCGCGCGGGCTGCGTGAAGCGTTGCTGATCGTCGCCtccttcct                                                                                                                                            <  2:4162366/150‑1 (MQ=255)
            cAACCTGTTCCGTCAACGCAGGCGTCGATTCGCGGGGAAGAGAGTGAGCAAATCGAGCTACTCAATATTCGCAAAGAAACGCATGAAGAATATGCGCTGAGTCGTCCGCGCGGGCTGCGTGAAGCGTTGCTGATCGTCGCCTCCTTCCTg                                                                                                                                           <  1:2655339/150‑1 (MQ=255)
            cAACCTGTTCCGTCAACGCAGGCGTCGATTCGCGGGGAAGAGAGTGAGCAAATCGAGCTACTCAATATTCGCAAAGAAACGCATGAAGAATATGCGCTGAGTCGTCCGCGCGGGCTGCGTGAAGCGTTGCTGATCGTCGCCTCCTTCCTg                                                                                                                                           >  1:272117/1‑150 (MQ=255)
              aCCTGTTCCGTCAACGCAGGCGTCGATTCGCGGGGAAGAGAGTGAGCAAATCGAGCTACTCAATATTCGCAAAGAAACGCATGAAGAATATGCGCTGAGTCGTCCGCGCGGGCTGCGTGAAGCGTTGCTGATCGTCGCCTCCTTCCTGAt                                                                                                                                         <  2:2485918/150‑1 (MQ=255)
              aCCTGTTCCGTCAACGCAGGCGTCGATTCGCGGGGAAGAGAGTGAGCAAATCGAGCTACTCAATATTCGCAAAGAAACGCATGAAGAATATGCGCTGAGTCGTCCGCGCGGGCTGCGTGAAGCGTTGCTGATCGTCGCCTCCTTCCTGAt                                                                                                                                         >  1:547028/1‑150 (MQ=255)
               ccTGTTCCGTCAACGCAGGCGTCGATTCGCGGGGAAGAGAGTGAGCAAATCGAGCTACTCAATATTCGCAAAGAAACGCATGAAGAATATGCGCTGAGTCGTCCGCGCGGGCTGCGTGAAGCGTTGCTGATCGTCGCCTCCTTCCTGATg                                                                                                                                        >  2:2608552/1‑150 (MQ=255)
                 tGTTCCGTCAACGCAGGCGTCGATTCGCGGGGAAGAGAGTGAGCAAATCGAGCTACTCAATATTCGCAAAGAAACGCATGAAGAATATGCGCTGAGTCGTCCGCGCGGGCTGCGTGAAGCGTTGCTGATCGTCGCCTCCTTCCTGATGtt                                                                                                                                      <  2:1715395/150‑1 (MQ=255)
                 tGTTCCGTCAACGCAGGCGTCGATTCGCGGGGAAGAGAGTGAGCAAATCGAGCTACTCAATATTCGCAAAGAAACGCATGAAGAATATGCGCTGAGTCGTCCGCGCGGGCTGCGTGAAGCGTTGCTGATCGTCGCCTCCTTCCTGATGtt                                                                                                                                      <  2:3256775/150‑1 (MQ=255)
                      cGTCAACGCAGGCGTCGATTCGCGGGGAAGAGAGTGAGCAAATCGAGCTACTCAATATTCGCAAAGAAACGCATGAAGAATATGCGCTGAGTCGTCCGCGCGGGCTGCGTGAAGCGTTGCTGATCGTCGCCTCCTTCCTGATGTTCtttt                                                                                                                                 >  1:3424517/1‑150 (MQ=255)
                      cGTCAACGCAGGCGTCGATTCGCGGGGAAGAGAGTGAGCAAATCGAGCTACTCAATATTCGCAAAGAAACGCATGAAGAATATGCGCTGAGTCGTCCGCGCGGGCTGCGTGAAGCGTTGCTGATCGTAGCCTCCTTCCTGATGTTCtttt                                                                                                                                 >  1:548377/1‑150 (MQ=255)
                       gTCAACGCAGGCGTCGATTCGCGGGGAAGAGAGTGAGCAAATCGAGCTACTCAATATTCGCAAAGAAACGCATGAAGAATATGCGCTGAGTCGTCCGCGCGGGCTGCGTGAAGCGTTGCTGATCGTCGCCTCCTTCCTGATGTTCttttt                                                                                                                                >  2:2789204/1‑150 (MQ=255)
                         cAACGCAGGCGTCGATTCGCGGGGAAGAGAGTGAGCAAATCGAGCTACTCAATATTCGCAAAGAAACGCATGAAGAATATGCGCTGAGTCGTCCGCGCGGGCTGCGTGAAGCGTTGCTGATCGTCGCCTCCTTCCTGATGTTCTTTTTCt                                                                                                                              <  2:2715538/150‑1 (MQ=255)
                         cAACGCAGGCGTCGATTCGCGGGGAAGAGAGTGAGCAAATCGAGCTACTCAATATTCGCAAAGAAACGCATGAAGAATATGCGCTGAGTCGTCCGCGCGGGCTGCGTGAAGCGTTGCTGATCGTCGCCTCCTTCCTGATGTTCTTTTTCt                                                                                                                              <  2:2414158/150‑1 (MQ=255)
                          aaCGCAGGCGTCGATTCGCGGGGAAGAGAGTGAGCAAATCGAGCTACTCAATATTCGCAAAGAAACGCATGAAGAATATGCGCTGAGTCGTCCGCGCGGGCTGCGTGAAGCGTTGCTGATCGTCGCCTCCTTCCTGATGTTCTTTTTCTg                                                                                                                             >  1:2108561/1‑150 (MQ=255)
                            cGCAGGCGTCGATTCGCGGGGAAGAGAGTGAGCAAATCGAGCTACTCAATATTCGCAAAGAAACGCATGAAGAATATGCGCTGAGTCGTCCGCGCGGGCTGCGTGAAGCGTTGCTGATCGTCGCCTCCTTCCTGATGTTCTTTTTCTGcc                                                                                                                           >  1:2180631/1‑150 (MQ=255)
                             gCAGGCGTCGATTCGCGGGGAAGAGAGTGAGCAAATCGAGCTACTCAATATTCGCAAAGAAACGCATGAAGAATATGCGCTGAGTCGTCCGCGCGGGCTGCGTGAAGCGTTGCTGATCGTCGCCTCCTTCCTGATGTTCTTTTTCTGCCt                                                                                                                          <  1:1594721/150‑1 (MQ=255)
                             gCAGGCGTCGATTCGCGGGGAAGAGAGTGAGCAAATCGAGCTACTCAATATTCGCAAAGAAACGCATGAAGAATATGCGCTGAGTCGTCCGCGCGGGCTGCGTGAAGCGTTGCTGATCGTCGCCTCCTTCCTGATGTTCTTTTTCTGCCt                                                                                                                          <  1:698003/150‑1 (MQ=255)
                               aGGCGTCGATTCGCGGGGAAGAGAGTGAGCAAATCGAGCTACTCAATATTCGCAAAGAAACGCATGAAGAATATGCGCTGAGTCGTCCGCGCGGGCTGCGTGAAGCGTTGCTGATCGTCGCCTCCTTCCTGATGTTCTTTTTCTGCCTGa                                                                                                                        >  1:1890446/1‑150 (MQ=255)
                                  cGTCGATTCGCGGGGAAGAGAGTGAGCAAATCGAGCTACTCAATATTCGCAAAGAAACGCATGAAGAATATGCGCTGAGTCGTCCGCGCGGGCTGCGTGAAGCGTTGCTGATCGTCGCCTCCTTCCTGATGTTCTTTTTCTGCCTGATTa                                                                                                                     <  1:473821/150‑1 (MQ=255)
                                  cGTCGATTCGCGGGGAAGAGAGTGAGCAAATCGAGCTACTCAATATTCGCAAAGAAACGCATGAAGAATATGCGCTGAGTCGTCCGCGCGGGCTGCGTGAAGCGTTGCTGATCGTCGCCTCCTTCCTGATGTTCTTTTTCTGCCTGATTa                                                                                                                     <  1:2519705/150‑1 (MQ=255)
                                   gTCGATTCGCGGGGAAGAGAGTGAGCAAATCGAGCTACTCAATATTCGCAAAGAAACGCATGAAGAATATGCGCTGAGTCGTCCGCGCGGGCTGCGTGAAGCGTTGCTGATCGTCGCCTCCTTCCTGATGTTCTTTTTCTGCCTGATTAc                                                                                                                    >  1:1953577/1‑150 (MQ=255)
                                      gATTCGCGGGGAAGAGAGTGAGCAAATCGAGCTACTCAATATTCGCAAAGAAACGCATGAAGAATATGCGCTGAGTCGTCCGCGCGGGCTGCGTGAAGCGTTGCTGATCGTCGCCTCCTTCCTGATGTTCTTTTTCTGCCTGATTAcccc                                                                                                                 >  1:4485594/1‑150 (MQ=255)
                                       aTTCGCGGGGAAGAGAGTGAGCAAATCGAGCTACTCAATATTCGCAAAGAAACGCATGAAGAATATGCGCTGAGTCGTCCGCGCGGGCTGCGTGAAGCGTTGCTGATCGTCGCCTCCTTCCTGATGTTCTTTTTCTGCCTGATTACCCCg                                                                                                                >  1:1024836/1‑150 (MQ=255)
                                          cgcgGGGAAGAGAGTGAGCAAATCGAGCTACTCAATATTCGCAAAGAAACGCATGAAGAATATGCGCTGAGTCGTCCGCGCGGGCTGCGTGAAGCGTTGCTGATCGTCGCCTCCTTCCTGATGTTCTTTTTCTGCCTGATTACCCCGGAt                                                                                                             <  2:3516768/150‑1 (MQ=255)
                                          cgcgGGGAAGAGAGTGAGCAAATCGAGCTACTCAATATTCGCAAAGAAACGCATGAAGAATATGCGCTGAGTCGTCCGCGCGGGCTGCGTGAAGCGTTGCTGATCGTCGCCTCCTTCCTGATGTTCTTTTTCTGCCTGATTACCCCGGAt                                                                                                             <  1:3965341/150‑1 (MQ=255)
                                             ggggAAGAGAGTGAGCAAATCGAGCTACTCAATATTCGCAAAGAAACGCATGAAGAATATGCGCTGAGTCGTCCGCGCGGGCTGCGTGAAGCGTTGCTGATCGTCGCCTCCTTCCTGATGTTCTTTTTCTGCCTGATTACCCCGGATGTa                                                                                                          <  1:3003039/150‑1 (MQ=255)
                                              gggAAGAGAGTGAGCAAATCGAGCTACTCAATATTCGCAAAGAAACGCATGAAGAATATGCGCTGAGTCGTCCGCGCGGGCTGCGTGAAGCGTTGCTGATCGTCGCCTCCTTCCTGATGTTCTTTTTCTGCCTGATTACCCCGGATGTAt                                                                                                         >  1:2947481/1‑150 (MQ=255)
                                                    agagTGAGCAAATCGAGCTACTCAATATTCGCAAAGAAACGCATGAAGAATATGCGCTGAGTCGTCCGCGCGGGCTGCGTGAAGCGTTGCTGATCGTCGCCTCCTTCCTGATGTTCTTTTTCTGCCTGATTACCCCGGATGTATTTGTTc                                                                                                   >  2:3825544/1‑150 (MQ=255)
                                                    agagTGAGCAAATCGAGCTACTCAATATTCGCAAAGAAACGCATGAAGAATATGCGCTGAGTCGTCCGCGCGGGCTGCGTGAAGCGTTGCTGATCGTCGCCTCCTTCCTGATGTTCTTTTTCTGCCTGATTACCCCGGATGTATTTGTTc                                                                                                   >  2:2608877/1‑150 (MQ=255)
                                                      agTGAGCAAATCGAGCTACTCAATATTCGCAAAGAAACGCATGAAGAATATGCGCTGAGTCGTCCGCGCGGGCTGCGTGAAGCGTTGCTGATCGTCGCCTCCTTCCTGATGTTCTTTTTCTGCCTGATTACCCCGGATGTATTTGTTCCg                                                                                                 >  2:423618/1‑150 (MQ=255)
                                                        tGAGCAAATCGAGCTACTCAATATTCGCAAAGAAACGCATGAAGAATATGCGCTGAGTCGTCCGCGCGGGCTGCGTGAAGCGTTGCTGATCGTCGCCTCCTTCCTGATGTTCTTTTTCTGCCTGATTACCCCGGATGTATTTGTTCCGtg                                                                                               >  1:799398/1‑150 (MQ=255)
                                                         gAGCAAATCGAGCTACTCAATATTCGCAAAGAAACGCATGAAGAATATGCGCTGAGTCGTCCGCGCGGGCTGCGTGAAGCGTTGCTGATCGTCGCCTCCTTCCTGATGTTCTTTTTCTGCCTGATTACCCCGGATGTATTTGTTCCGtgg                                                                                              <  2:1050345/150‑1 (MQ=255)
                                                            cAAATCGAGCTACTCAATATTCGCAAAGAAACGCATGAAGAATATGCGCTGAGTCGTCCGCGCGGGCTGCGTGAAGCGTTGCTGATCGTCGCCTCCTTCCTGATGTTCTTTTTCTGCCTGATTACCCCGGATGTATTTGTTCCGtggctg                                                                                           >  1:3278600/1‑150 (MQ=255)
                                                            cAAATCGAGCTACTCAATATTCGCAAAGAAACGCATGAAGAATATGCGCTGAGTCGTCCGCGCGGGCTGCGTGAAGCGTTGCTGATCGTCGCCTCCTTCCTGATGTTCTTTTTCTGCCTGATTACCCCGGATGAATTTGTTCCGtggctg                                                                                           <  1:1352135/150‑1 (MQ=255)
                                                            cAAATCGAGCTACTCAATATTCGCAAAGAAACGCATGAAGAATATGCGCTGAGTCGTCCGCGCGGGCTGCGTGAAGCGTTGCTGATCGTAGCCTCCTTCCTGATGTTCTTTTTCTGCCTGATTACCCCGGATGTATTTGTTCCGtggctg                                                                                           <  2:4061465/150‑1 (MQ=255)
                                                                 cGAGCTACTCAATATTCGCAAAGAAACGCATGAAGAATATGCGCTGAGTCGTCCGCGCGGGCTGCGTGAAGCGTTGCTGATCGTCGCCTCCTTCCTGATGTTCTTTTTCTGCCTGATTACCCCGGATGTATTTGTTCCGTGGCTGGCAgg                                                                                      >  2:1669142/1‑150 (MQ=255)
                                                                   aGCTACTCAATATTCGCAAAGAAACGCATGAAGAATATGCGCTGAGTCGTCCGCGCGGGCTGCGTGAAGCGTTGCTGATCGTCGCCTCCTTCCTGATGTTCTTTTTCTGCCTGATTACCCCGGATGTATTTGTTCCGTGGCTGGCAggcg                                                                                    <  1:1501328/150‑1 (MQ=255)
                                                                      tACTCAATATTCGCAAAGAAACGCATGAAGAATATGCGCTGAGTCGTCCGCGCGGGCTGCGTGAAGCGTTGCTGATCGTCGCCTCCTTCCTGATGTTCTTTTTCTGCCTGATTACCCCGGATGTATTTGTTCCGTGGCTGGCAGgcggcg                                                                                 >  1:1100589/1‑150 (MQ=255)
                                                                      tACTCAATACTCGCAAAGAAACGCATGAAGAATATGCGCTGAGTCGTCCGCGCGGGCTGCGTGAAGCGTTGCTGATCGTCGCCTCCTTCCTGATGTTCTTTTTCTGCCTGATTACCCCGGATGTATTTGTTCCGTGGCTGGCAGgcggcg                                                                                 >  1:3226072/1‑150 (MQ=255)
                                                                        cTCAATATTCGCAAAGAAACGCATGAAGAATATGCGCTGAGTCGTCCGCGCGGGCTGCGTGAAGCGTTGCTGATCGTCGCCTCCTTCCTGATGTTCTTTTTCTGCCTGATTACCCCGGATGTATTTGTTCCGTGGCTGGCAGGCGGcgcg                                                                               >  2:3078287/1‑150 (MQ=255)
                                                                        cTCAATATTCGCAAAGAAACGCATGAAGAATATGCGCTGAGTCGTCCGCGCGGGCTGCGTGAAGCGTTGCTGATCGTCGCCTCCTTCCTGATGTTCTTTTTCTGCCTGATTACCCCGGATGTATTTGTTCCGTGGCTGGCAGGCGGcgcg                                                                               >  1:2223678/1‑150 (MQ=255)
                                                                           aaTATTCGCAAAGAAACGCATGAAGAATATGCGCTGAGTCGTCCGCGCGGGCTGCGTGAAGCGTTGCTGATCGTCGCCTCCTTCCTGATGTTCTTTTTCTGCCTGATTACCCCGGATGTATTTGTTCCGTGGCTGGCAGGCGGCGCGTTa                                                                            >  1:3692971/1‑150 (MQ=255)
                                                                           aaTATTCGCAAAGAAACGCATGAAGAATATGCGCTGAGTCGTCCGCGCGGGCTGCGTGAAGCGTTGCTGATCGTCGCCTCCTTCCTGATGTTCTTTTTCTGCCTGATTACCCCGGATGTATTTGTTCCGTGGCTGGCAGGCGGCGCGTTa                                                                            >  2:1016027/1‑150 (MQ=255)
                                                                             tatTCGCAAAGAAACGCATGAAGAATATGCGCTGAGTCGTCCGCGCGGGCTGCGTGAAGCGTTGCTGATCGTCGCCTCCTTCCTGATGTTCTTTTTCTGCCTGATTACCCCGGATGTATTTGTTCCGTGGCTGGCAGGCGGCGCGTTAct                                                                          <  2:547028/150‑1 (MQ=255)
                                                                              atTCGCAAAGAAACGCATGAAGAATATGCGCTGAGTCGTCCGCGCGGGCTGCGTGAAGCGTTGCTGATCGTCGCCTCCTTCCTGATGTTCTTTTTCTGCCTGATTACCCCGGATGTATTTGTTCCGTGGCTGGCAGGCGGCGCGTTActg                                                                         >  1:4309866/1‑150 (MQ=255)
                                                                              atTCGCAAAGAAACGCATGAAGAATATGCGCTGAGTCGTCCGCGCGGGCTGCGTGAAGCGTTGCTGATCGTCGCCTCCTTCCTGATGTTCTTTTTCTGCCTGATTACCCCGGATGTATTTGTTCCGTGGCTGGCAGGCGGCGCGTTActg                                                                         >  1:4043839/1‑150 (MQ=255)
                                                                              atTCGCAAAGAAACGCATGAAGAATATGCGCTGAGTCGTCCGCGCGGGCTGCGTGAAGCGTTGCTGATCGTCGCCTCCTTCCTGATGTTCTTTTTCTGCCTGATTACCCCGGATGTAGTTGTTCCGTGGCTGGCAGGCGGCGCGTTActg                                                                         >  1:4149151/1‑150 (MQ=255)
                                                                                tCGCAAAGAAACGCATGAAGAATATGCGCTGAGTCGTCCGCGCGGGCTGCGTGAAGCGTTGCTGATCGTCGCCTCCTTCCTGATGTTCTTTTTCTGCCTGATTACCCCGGATGTATTTGTTCCGTGGCTGGCAGGCGGCGCGTTActgct                                                                       <  1:3130794/150‑1 (MQ=255)
                                                                                 cGCAAAGAAACGCATGAAGAATATGCGCTGAGTCGTCCGCGCGGGCTGCGTGAAGCGTTGCTGATCGTCGCCTCCTTCCTGATGTTCTTTTTCTGCCTGATTACCCCGGATGTATTTGTTCCGTGGCTGGCAGGCGGCGCGTTActgctg                                                                      <  2:2611720/150‑1 (MQ=255)
                                                                                 cGCAAAGAAACGCATGAAGAATATGCGCTGAGTCGTCCGCGCGGGCTGCGTGAAGCGTTGCTGATCGTCGCCTCCTTCCTGATGTTCTTTTTCTGCCTGATTACCCCGGATGTATTTGTTCCGTGGCTGGCAGGCGGCGCGTTActgctg                                                                      >  2:3280526/1‑150 (MQ=255)
                                                                                     aaGAAACGCATGAAGAATATGCGCTGAGTCGTCCGCGCGGGCTGCGTGAAGCGTTGCTGATCGTCGCCTCCTTCCTGATGTTCTTTTTCTGCCTGATTACCCCGGATGTATTTGTTCCGTGGCTGGCAGGCGGCGCGTTACTGCTGCTgg                                                                  <  2:412413/150‑1 (MQ=255)
                                                                                     aaGAAACGCATGAAGAATATGCGCTGAGTCGTCCGCGCGGGCTGCGTGAAGCGTTGCTGATCGTCGCCTCCTTCCTGATGTTCTTTTTCTGCCTGATTACCCCGGATGTATTTGTTCCGTGGCTGGCAGGCGGCGCGTTACTGCTGCTgg                                                                  >  1:208505/1‑150 (MQ=255)
                                                                                     aaGAAACGCATGAAGAATATGCGCTGAGTCGTCCGCGCGGGCTGCGTGAAGCGTTGCTGATCGTCGCCTCCTTCCTGATGTTCTTTTTCTGCCTGATTACCCCGGATGAATTTGTTCCGTGGCTGGCAGGCGGCGCGTTACTGCTGCTgg                                                                  >  1:405392/1‑150 (MQ=255)
                                                                                        aaaCGCATGAAGAATATGCGCTGAGTCGTCCGCGCGGGCTGCGTGAAGCGTTGCTGATCGTCGCCTCCTTCCTGATGTTCTTTTTCTGCCTGATTACCCCGGATGAATTTGTTCCGTGGCTGGCAGGCGGCGCGTTACTGCTGCTGGgcg                                                               <  1:3701469/150‑1 (MQ=255)
                                                                                          aCGCATGAAGAATATGCGCTGAGTCGTCCGCGCGGGCTGCGTGAAGCGTTGCTGATCGTCGCCTCCTTCCTGATGTTCTTTTTCTGCCTGATTACCCCGGATGTATTTGTTCCGTGGCTGGCAGGCGGCGCGTTACTGCTGCTGGGCGCa                                                             >  1:1311041/1‑150 (MQ=255)
                                                                                           cGCATGAAGAATATGCGCTGAGTCGTCCGCGCGGGCTGCGTGAAGCGTTGCTGATCGTCGCCTCCTTCCTGATGTTCTTTTTCTGCCTGATTACCCCGGATGTATTTGTTCCGTGGCTGGCAGGCGGCGCGTTACTGCTGCTGGGCGCAg                                                            >  2:675762/1‑150 (MQ=255)
                                                                                           cGCATGAAGAATATGCGCTGAGTCGTCCGCGCGGGCTGCGTGAAGCGTTGCTGATCGTCGCCTCCTTCCTGATGTTCTTTTTCTGCCTGATTACCCCGGATGTATTTGTTCCGTGGCTGGCAGGCGGCGCGTTACTGCTGCTGGGCGCAg                                                            >  2:3757779/1‑150 (MQ=255)
                                                                                               tGAAGAATATGCGCTGAGTCGTCCGCGCGGGCTGCGTGAAGCGTTGCTGATCGTCGCCTCCTTCCTGATGTTCTTTTTCTGCCTGATTACCCCGGATGTATTTGTTCCGTGGCTGGCAGGCGGCGCGTTACTGCTGCTGGGCGCAGGTCt                                                        <  2:230847/150‑1 (MQ=255)
                                                                                                gaagaaTATGCGCTGAGTCGTCCGCGCGGGCTGCGTGAAGCGTTGCTGATCGTCGCCTCCTTCCTGATGTTCTTTTTCTGCCTGATTACCCCGGATGTATTTGTTCCGTGGCTGGCAGGCGGCGCGTTACTGCTGCTGGGCGCAGGTCtg                                                       >  2:1436184/1‑150 (MQ=255)
                                                                                                  agaaTATGCGCTGAGTCGTCCGCGCGGGCTGCGTGAAGCGTTGCTGATCGTCGCCTCCTTCCTGATGTTCTTTTTCTGCCTGATTACCCCGGATGTATTTGTTCCGTGGCTGGCAGGCGGCGCGTTACTGCTGCTGGGCGCAGGTCtgtg                                                     >  1:2201586/1‑150 (MQ=255)
                                                                                                  agaaTATGCGCTGAGTCGTCCGCGCGGGCTGCGTGAAGCGTTGCTGATCGTCGCCTCCTTCCTGATGTTCTTTTTCTGCCTGATTACCCCGGATGTATTTGTTCCGTGGCTGGCAGGCGGCGCGTTACTGCTGCTGGGCGCAGGTCtgtg                                                     >  2:3444616/1‑150 (MQ=255)
                                                                                                   gaaTATGCGCTGAGTCGTCCGCGCGGGCTGCGTGAAGCGTTGCTGATCGTCGCCTCCTTCCTGATGTTCTTTTTCTGCCTGATTACCCCGGATGTATTTGTTCCGTGGCTGGCAGGCGGCGCGTTACTGCTGCTGGGCGCAGGTCTGTgg                                                    <  2:2932673/150‑1 (MQ=255)
                                                                                                    aaTATGCGCTGAGTCGTCCGCGCGGGCTGCGTGAAGCGTTGCTGATCGTCGCCTCCTTCCTGATGTTCTTTTTCTGCCTGATTACCCCGGATGTATTTGTTCCGTGGCTGGCAGGCGGCGCGTTACTGCTGCTGGGCGCAGGTCTGTggg                                                   >  2:4492641/1‑150 (MQ=255)
                                                                                                    aaTATGCGCTGAGTCGTCCGCGCGGGCTGCGTGAAGCGTTGCTGATCGTCGCCTCCTTCCTGATGTTCTTTTTCTGCCTGATTACCCCGGATGTATTTGTTCCGTGGCTGGCAGGCGGCGCGTTACTGCTGCTGGGCGCAGGTCTGTggg                                                   <  2:1577078/150‑1 (MQ=255)
                                                                                                    aaTATGCGCTGAGTCGTCCGCGCGGGCTGCGTGAAGCGTTGCTGATCGTAGCCTCCTTCCTGATGTTCTTTTTCTGCCTGATTACCCCGGATGTATTTGTTCCGTGGCTGGCAGGCGGCGCGTTACTGCTGCTGGGCGCAGGTCTGTggg                                                   >  1:2799508/1‑150 (MQ=255)
                                                                                                       atGCGCTGAGTCGTCCGCGCGGGCTGCGTGAAGCGTTGCTGATCGTCGCCTCCTTCCTGATGTTCTTTTTCTGCCTGATTACCCCGGATGTATTTGTTCCGTGGCTGGCAGGCGGCGCGTTACTGCTGCTGGGCGCAGGTCTGTGGGGGc                                                >  2:3944387/1‑150 (MQ=255)
                                                                                                        tGCGCTGAGTCGTCCGCGCGGGCTGCGTGAAGCGTTGCTGATCGTCGCCTCCTTCCTGATGTTCTTTTTCTGCCTGATTACCCCGGATGTATTTGTTCCGTGGCTGGCAGGCGGCGCGTTACTGCTGCTGGGCGCAGGTCTGTGGGGGCt                                               >  1:1736620/1‑150 (MQ=255)
                                                                                                         gcgcTGAGTCGTCCGCGCGGGCTGCGTGAAGCGTTGCTGATCGTCGCCTCCTTCCTGATGTTCTTTTTCTGCCTGATTACCCCGGATGTATTTGTTCCGTGGCTGGCAGGCGGCGCGTTACTGCTGCTGGGCGCAGGTCTGTGGGGGCTa                                              <  1:4192667/150‑1 (MQ=255)
                                                                                                            cTGAGTCGTCCGCGCGGGCTGCGTGAAGCGTTGCTGATCGTCGCCTCCTTCCTGATGTTCTTTTTCTGCCTGATTACCCCGGATGTATTTGTTCCGTGGCTGGCAGGCGGCGCGTTACTGCTGCTGGGCGCAGGTCTGTGGGGGCTATTc                                           <  2:1946276/150‑1 (MQ=255)
                                                                                                              gAGTCGTCCGCGCGGGCTGCGTGAAGCGTTGCTGATCGTCGCCTCCTTCCTGATGTTCTTTTTCTGCCTGATTACCCCGGATGTATTTGTTCCGTGGCTGGCAGGCGGCGCGTTACTGCTGCTGGGCGCAGGTCTGTGGGGGCTATTcgc                                         >  1:885533/1‑150 (MQ=255)
                                                                                                               aGTCGTCCGCGCGGGCTGCGTGAAGCGTTGCTGATCGTCGCCTCCTTCCTGATGTTCTTTTTCTGCCTGATTACCCCGGATGTATTTGTTCCGTGGCTGGCAGGCGGCGCGTTACTGCTGCTGGGCGCAGGTCTGTGGGGGCTATTcgcg                                        >  1:3449151/1‑150 (MQ=255)
                                                                                                                   gtcCGCGCGGGCTGCGTGAAGCGTTGCTGATCGTCGCCTCCTTCCTGATGTTCTTTTTCTGCCTGATTACCCCGGATGTATTTGTTCCGTGGCTGGCAGGCGGCGCGTTACTGCTGCTGGGCGCAGGTCTGTGGGGGCTATTCGCGcccc                                    >  2:3266558/1‑150 (MQ=255)
                                                                                                                   gtcCGCGCGGGCTGCGTGAAGCGTTGCTGATCGTCGCCTCCTTCCTGATGTTCTTTTTCTGCCTGATTACCCCGGATGTATTTGTTCCGTGGCTGGCAGGCGGCGCGTTACTGCTGCTGGGCGCAGGTCTGTGGGGGCTATTCGCGcccc                                    >  2:1276697/1‑150 (MQ=255)
                                                                                                                   gtcCGCGCGGGCTGCGTGAAGCGTTGCTGATCGTCGCCTCCTTCCTGATGTTCTTTTTCTGCCTGATTACCCCGGATGTATTTGTTCCGTGGCTGGCAGGCGGCGCGTTACTGCTGCTGGGCGCAGGTCTGTGGGGGCTATTCGCGcccc                                    >  1:961675/1‑150 (MQ=255)
                                                                                                                    tcCGCGCGGGCTGCGTGAAGCGTTGCTGATCGTCGCCTCCTTCCTGATGTTCTTTTTCTGCCTGATTACCCCGGATGTATTTGTTCCGTGGCTGGCAGGCGGCGCGTTACTGCTGCTGGGCGCAGGTCTGTGGGGGCTATTCGCGccccc                                   <  2:4114721/150‑1 (MQ=255)
                                                                                                                     ccGCGCGGGCTGCGTGAAGCGTTGCTGATCGTCGCCTCCTTCCTGATGTTCTTTTTCTGCCTGATTACCCCGGATGTATTTGTTCCGTGGCTGGCAGGCGGCGCGTTACTGCTGCTGGGCGCAGGTCTGTGGGGGCTATTCGCGCCCCCg                                  >  2:714870/1‑150 (MQ=255)
                                                                                                                           gggCTGCGTGAAGCGTTGCTGATCGTCGCCTCCTTCCTGATGTTCTTTTTCTGCCTGATTACCCCGGATGTATTTGTTCCGTGGCTGGCAGGCGGCGCGTTACTGCTGCTGGGCGCAGGTCTGTGGGGGCTATTCGCGCCCCCGGCaaaa                            >  2:606090/1‑150 (MQ=255)
                                                                                                                               tGCGTGAAGCGTTGCTGATCGTCGCCTCCTTCCTGATGTTCTTTTTCTGCCTGATTACCCCGGATGTATTTGTTCCGTGGCTGGCAGGCGGCGCGTTACTGCTGCTGGGCGCAGGTCTGTGGGGGCTATTCGCGCCCCCGGCAAAAtcct                        <  2:1850247/150‑1 (MQ=255)
                                                                                                                                 cGTGAAGCGTTGCTGATCGTCGCCTCCTTCCTGATGTTCTTTTTCTGCCTGATTACCCCGGATGTATTTGTTCCGTGGCTGGCAGGCGGCGCGTTACTGCTGCTGGGCGCAGGTCTGTGGGGGCTATTCGCGCCCCCGGCAAAAtcctcc                      <  1:732324/150‑1 (MQ=255)
                                                                                                                                  gTGAAGCGTTGCTGATCGTCGCCTCCTTCCTGATGTTCTTTTTCTGCCTGATTACCCCGGATGTATTTGTTCCGTGGCTGGCAGGCGGCGCGTTACTGCTGCTGGGCGCAGGTCTGTGGGGGCTATTCGCGCCCCCGGCAAAATCCTccc                     <  1:2113059/150‑1 (MQ=255)
                                                                                                                                  gTGAAGCGTTGCTGATCGTCGCCTCCTTCCTGATGTTCTTTTTCTGCCTGATTACCCCGGATGTATTTGTTCCGTGGCTGGCAGGCGGCGCGTTACTGCTGCTGGGCGCAGGTCTGTGGGGGCTATTCGCGCCCCCGGCAAAATCCTccc                     >  2:1499995/1‑150 (MQ=255)
                                                                                                                                   tGAAGCGTTGCTGATCGTCGCCTCCTTCCTGATGTTCTTTTTCTGCCTGATTACCCCGGATGTATTTGTTCCGTGGCTGGCAGGCGGCGCGTTACTGCTGCTGGGCGCAGGTCTGTGGGGGCTATTCGCGCCCCCGGCAAAATCCTCCCt                    <  1:3855273/150‑1 (MQ=255)
                                                                                                                                   tGAAGCGTTGCTGATCGTCGCCTCCTTCCTGATGTTCTTTTTCTGCCTGATTACCCCGGATGTATTTGTTCCGTGGCTGGCAGGCGGCGCGTTACTGCTGCTGGGCGCAGGTCTGTGGGGGCTATTCGCGCCCCCGGCAAAATCCTCCCt                    >  1:3637490/1‑150 (MQ=255)
                                                                                                                                   tGAAGCGTTGCTGATCGTCGCCTCCTTCCTGATGTTCTTTTTCTGCCTGATTACCCCGGATGTATTTGTTCCGTGGCTGGCAGGCGGCGCGTTACTGCTGCTGGGCGCAGGTCTGTGGGGGCTATTCGCGCCCCCGGCAAAATCCTCCCt                    <  1:3405648/150‑1 (MQ=255)
                                                                                                                                        cGTTGCTGATCGTCGCCTCCTTCCTGATGTTCTTTTTCTGCCTGATTACCCCGGATGTATTTGTTCCGTGGCTGGCAGGCGGCGCGTTACTGCTGCTGGGCGCAGGTCTGTGGGGGCTATTCGCGCCCCCGGCAAAATCCTCCCTGCggg               >  2:2731908/1‑150 (MQ=255)
                                                                                                                                         gTTGCTGATCGTCGCCTCCTTCCTGATGTTCTTTTTCTGCCTGATTACCCCGGATGTATTTGTTCCGTGGCTGGCAGGCGGCGCGTTACTGCTGCTGGGCGCAGGTCTGTGGGGGCTATTCGCGCCCCCGGCAAAATCCTCCCTGCGGGa              >  2:1264043/1‑150 (MQ=255)
                                                                                                                                         gTTGCTGATCGTCGCCTCCTTCCTGATGTTCTTTTTCTGCCTGATTACCCCGGATGTATTTGTTCCGTGGCTGGCAGGCGGCGCGTTACTGCTGCTGGGCGCAGGTCTGTGGGGGCTATTCGCGCCCCCGGCAAAATCCTCCCTGCGGGa              <  2:2210221/150‑1 (MQ=255)
                                                                                                                                         gTTGCTGATCGTCGCCTCCTTCCTGATGTTCTTTTTCTGCCTGATTACCCCGGATGTATTTGTTCCGTGGCTGGCAGGCGGCGCGTTACTGCTGCTGGGCGCAGGTCTGTGGGGGCTATTCGCGCCCCCGGCAAAATCCTCCCTGCGGGa              <  1:2610677/150‑1 (MQ=255)
                                                                                                                                           tGCTGATCGTCGCCTCCTTCCTGATGTTCTTTTTCTGCCTGATTACCCCGGATGTATTTGTTCCGTGGCTGGCAGGCGGCGCGTTACTGCTGCTGGGCGCAGGTCTGTGGGGGCTATTCGCGCCCCCGGCAAAATCCTCCCTGCGGGaaa            <  2:4358541/150‑1 (MQ=255)
                                                                                                                                              tGATCGTCGCCTCCTTCCTGATGTTCTTTTTCTGCCTGATTACCCCGGATGTATTTGTTCCGTGGCTGGCAGGCGGCGCGTTACTGCTGCTGGGCGCAGGTCTGTGGGGGCTATTCGCGCCCCCGGCAAAATCCTCCCTGCGGGAAATTc         >  1:2890668/1‑150 (MQ=255)
                                                                                                                                                aTCGTCGCCTCCTTCCTGATGTTCTTTTTCTGCCTGATTACCCCGGATGTATTTGTTCCGTGGCTGGCAGGCGGCGCGTTACTGCTGCTGGGCGCAGGTCTGTGGGGGCTATTCGCGCCCCCGGCAAAATCCTCCCTGCGGGAAATTCAt       >  1:2484666/1‑150 (MQ=255)
                                                                                                                                                  cgtcgCCTCCTTCCTGATGTTCTTTTTCTGCCTGATTACCCCGGATGTATTTGTTCCGTGGCTGGCAGGCGGCGCGTTACTGCTGCTGGGCGCAGGTCTGTGGGGGCTATTCGCGCCCCCGGCAAAATCCTCCCTGCGGGACATTCATTg     >  2:1941941/1‑150 (MQ=255)
                                                                                                                                                     cgCCTCCTTCCTGATGTTCTTTTTCTGCCTGATTACCCCGGATGTATTTGTTCCGTGGCTGGCAGGCGGCGCGTTACTGCTGCTGGGCGCAGGTCTGTGGGGGCTATTCGCGCCCCCGGCAAAATCCTCCCTGCGGGAAATTCATTGTCt  <  1:1488960/150‑1 (MQ=255)
                                                                                                                                                     cgCCTCCTTCCTGATGTTCTTTTTCTGCCTGATTACCCCGGATGTATTTGTTCCGTGGCTGGCAGGCGGCGCGTTACTGCTGCTGGGCGCAGGTCTGTGGGGGCTATTCGCGCCCCCGGCAAAATCCTCCCTGCGGGAAATTCATTGTCt  <  1:1125139/150‑1 (MQ=255)
                                                                                                                                                     cgCCTCCTTCCTGATGTTCTTTTTCTGCCTGATTACCCCGGATGTATTTGTTCCGTGGCTGGCAGGCGGCGCGTTACTGCTGCTGGGCGCAGGTCTGTGGGGGCTATTCGCGCCCCCGGCAAAATCCTCCCTGCGGGAAATTCATTGTCt  >  1:1820089/1‑150 (MQ=255)
                                                                                                                                                     cgCCTCCTTCCTGATGTTCTTTTTCTGCCTGATTACCCCGGATGTATTTGTTCCGTGGCTGGCAGGCGGCGCGTTACTGCTGCTGGGCGCAGGTCTGTGGGGGCTATTCGCGCCCCCGGCAAAATCCTCCCTGCGGGAAATTCATTGTCt  <  2:3424517/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
AGCTATGCCTTGCAACCTGTTCCGTCAACGCAGGCGTCGATTCGCGGGGAAGAGAGTGAGCAAATCGAGCTACTCAATATTCGCAAAGAAACGCATGAAGAATATGCGCTGAGTCGTCCGCGCGGGCTGCGTGAAGCGTTGCTGATCGTCGCCTCCTTCCTGATGTTCTTTTTCTGCCTGATTACCCCGGATGTATTTGTTCCGTGGCTGGCAGGCGGCGCGTTACTGCTGCTGGGCGCAGGTCTGTGGGGGCTATTCGCGCCCCCGGCAAAATCCTCCCTGCGGGAAATTCATTGTCT  >  CP009273/3520308‑3520606

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑