Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,525,258 T→G 5.8% T181P (ACC→CCC)  yhgE ← DUF4153 family putative inner membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,525,2580TG 5.8% 701.7 / 7.6 244T181P (ACC→CCC) yhgEDUF4153 family putative inner membrane protein
Reads supporting (aligned to +/- strand):  ref base T (101/122);  new base G (19/2);  total (120/124)
Fisher's exact test for biased strand distribution p-value = 5.06e-05
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 251 total aligned reads displayed.

AGGGTTAGCAATGATACTAACGGCAGCAACCCCGTGGCGATTAACGTGAACAACTTTTGAATAGAGTCGATTAAACGTGACTGTGTTCGCGCGAGGATCACCGCCAGCGCGGTAACCAGACCTAACGTGAGATAAATAAACCAGT‑CGGTTGCAAAAAAGAGCGTATTGAAAAACGTGATGCCAACAAGTTTAAACAACTCACTCCACAGTAAAAGCACCAGCCACGTCAAGCCATTGGCGAGAAAAATCACCAGTAATATAAGTACGTTATGCCATACTGACTGGTAAAAATAGCGAT  >  CP009273/3525110‑3525407
                                                                                                                                                     |                                                                                                                                                     
aGGGTTAGCAATGATACTAACGGCAGCAACCCCGTGGCGATTAACGTGAACAACTTTTGAATAGAGTCGATTAAACGTGACTGTGTTCGCGCGAGGATCACCGCCAGCGCGGTAACCAGACCTAACGTGAGATAAATAAACCAGT‑CGGtt                                                                                                                                                      <  2:668176/150‑1 (MQ=255)
aGGGTTAGCAATGATACTAACGGCAGCAACCCCGTGGCGATTAACGTGAACAACTTTTGAATAGAGTCGATTAAACGTGACAGTGTTCGCGCGAGGATCACCGCCCGCGCGGGAACCAGACCAAACGTGAGATAAATAAAACACG‑CGGtt                                                                                                                                                      >  1:3625886/1‑150 (MQ=255)
 gggTTAGAAATGATACTAACGGCAGCAACCCCGTGGCGATTAACGTGAACAACTTTTGAATAGAGTCTATTAAACGTGACTGTGTTCGCGCGAGGATCACCGCCAGCGCGGTAACCAGACCTAACGTGAGATAAATAAACCAGT‑CGGTTg                                                                                                                                                     <  1:3880808/150‑1 (MQ=255)
     tAGCAATGATACTAACGGCAGCAACCCCGTGGCGATTAACGTGAACAACTTTTGAATAGAGTCGATTTAAAGTGACAGTGTTGGCGCCAGGGTAACCCCCCCCCCGGGAACACCAACAAAACTGAGATAAATAAAACACT‑CCGGTGGaaa                                                                                                                                                 >  1:2351100/1‑150 (MQ=255)
      aGCAATGATACTAACGGCAGCAACCCCGTGGCGATTAACGTGAACAACTTTTGAATAGAGTCGAGTAAACGGGACTGTGTTCGCGCGAGGATAACCGCCAGCCCGGTAACCAGACCAAACCTGAGATAAATAAAACAGT‑CGGGTGCaaaa                                                                                                                                                >  1:2224761/1‑150 (MQ=255)
         aaTGATACTAACGGCAGCAACCCCGTGGCGATTAACGTGAACAACTTTTGAATAGAGTCGATTAAACGTGACTGTGTTCGCGCGAGGATCACCGCCAGCGCGGTAACCAGACCTAACGTGAGATAAATAAACCAGT‑CGGTTGCAAAAAag                                                                                                                                             <  2:1478893/150‑1 (MQ=255)
         aaTGATACTAACGGCAGCAACCCCGTGGCGATTAACGTGAACAACTTTTGAATAGAGTCGATTAAACGTGACTGTGTTCGCGCGAGGATCACCGCCAGCGCGGTAACCAGACCTAACGTGAGATAAATAAACCAGT‑CGGTTGCAAAAAag                                                                                                                                             <  1:1402343/150‑1 (MQ=255)
          aTGATACTAACGGCAGCAACCCTGTGGCGATTAACGTGAACAACTTTTGAATAGAGTCGATTAAACGTGACTGTGTTCGCGCGAGGATCACCGCCAGCGCGGTAACCAGACCTAACGTGAGATAAATAAAACAGT‑CGGTTGCAAAAAaga                                                                                                                                            >  2:198591/1‑150 (MQ=255)
          aTGATACTAACGGCAGCAACCCTGTGGCGATTAACGTGAACAACTTTTGAATAGAGTCGATTAAAAGTGACTGTGTTCGCGCGAGGAGCACCGCCAGCGCGGTAACCAGACCAAACGTGAGATAAAAAAAACAGT‑CGGGTGCAAAAAaga                                                                                                                                            >  2:201244/1‑150 (MQ=255)
           tGATACTAACGGCAGCAACCCCGTGGCGATTAACGTGAACAACTTTTGAATAGAGTCGATTAAACGTGACTGTGTTCGCGCGAGGATCACCGCCAGCGCGGTAACCAGACCTACCGTGAGATAAATAAACCAGT‑CGGGTGCAAAAAagag                                                                                                                                           <  2:556355/150‑1 (MQ=255)
           tGATACTAACGGCAGCAACCCCGTGGCGATTAACGTGAACAACTTTTGAATAGAGTCGAGTTAAAGTGACTGTGTTCGCGCGAGGATCACCCCCAGCGCGGTAACCAGACCAAAAGTGAGATAAATAAAACAGT‑CGGTTGCAAAAAatag                                                                                                                                           >  1:1642004/1‑150 (MQ=255)
            gATACTAACGGCAGCAACCCCGTGGCGATTAACGTGAACAACTTTTGAATAGAGTCGATTAAACGTGACTGTGTTCGCGCGAGGATCACCGCCAGCGCGGTAACCAGACCTAACGTGAGATAAATAAACCAGT‑CGGTTGCAAAAAAGAGc                                                                                                                                          <  1:1765162/150‑1 (MQ=255)
               aCTAACGGCAGCAACCCCGTGGCGATTAACGTGAACAACTTTTGAATAGAGTCGATTAAACGTGACTGTGTTCGCGCGAGGATCACCGCCAGCGCGGTAACCAGACCTAACGTGAGATAAATAAACCAGT‑CGGTTGCAAAAAAGAGCGTa                                                                                                                                       <  2:155209/150‑1 (MQ=255)
               aCTAACGGCAGCAACCCCGTGGCGATTAACGTGAACAACTTTTGAATAGAGTCGATTAAAAGGGTCAGTGTTCGCGCGAGGATCAACGCCACCGCGGTAACCACACCTAACCTGAGATTAAAAAAACACT‑CGGGTGGAAAAAAGATCGtc                                                                                                                                       >  1:1611894/1‑149 (MQ=255)
               aCTAACGGCAGCAACCCCGTGGCGATTAACGTGAACAACTTTTGAATAGAGTCGAGTAAACGTGGCAGTGTTCGCGCGAGGGTCACCCCCACCCCGGTAACCAGACCCAAACGGAGATAAATAAAACACT‑CGGGTTCaaaaaaaatcgga                                                                                                                                       >  1:2053172/1‑143 (MQ=255)
                cTAACGGCAGCAACCCCGTGGCGATTAACGTGAACAACTTTTGAATAGAGTCGATTAAACGTGTCTGTGTTCGCGCGAGGATCACCGCCAGCGCGGTAACCAGACCTAACGGGAGATAAATAAACCAGT‑CGGGTGCAAAAAAGAGCGTAt                                                                                                                                      >  2:2474098/1‑150 (MQ=255)
                cTAACGGCAGCAACCCCGTGGCGATTAACGTGAACAACTTTTGAATAGAGTCGATTAAACGTGACTGTGTTCGCGCGAGGATCACCGCCAGCGCGGTAACCAGACCTAACGTGAGATAAATAAACCAGT‑CGGTTGCAAAAAAGAGCGTAt                                                                                                                                      <  2:384367/150‑1 (MQ=255)
                cTAACGGCAGCAACCCCGTGGCGATTAACGTGAACAACTTTTGAATAGAGTCGATTAAAAGTGTCTGTGTTCGCGCGCGGATCACCGCCCCCGCGGTAACCCGACCTAACGGGAGATAAATAAACCAGT‑CGGGTGCAAAAAAGAGCGTAt                                                                                                                                      >  2:518117/1‑150 (MQ=255)
                 tAACGGCAGCAACCCCGTGGCGATTAACGTGAACAACTTTTGAATAGAGTCGATTAAACGTGTCTGTGTTCGCGCGAGGATCACCGCCCGCGCGGTCACCAGACCGAACCTGAGAGAAATAAACCAGT‑CGGTTGCAAAAAAGAGCGGAtt                                                                                                                                     >  1:2889574/1‑150 (MQ=255)
                  aaCGGCAGCAACCCCGTGGCGATTAACGTGAACAACTTTTGAATAGAGTCGATTAAACGTGACTGTGTTCGCGCGAGGATCACCGCCAGCGCGGTAACCAGACCTAACGTGAGATAAATAAACCAGT‑CGGTTGCAAAAAAGAGCGTATTg                                                                                                                                    <  2:3740883/150‑1 (MQ=255)
                  aaCGGCAGCAACCCCGTGGCGATTAACGTGAACAACTTTTGAATAGAGTCGATTAAACGTGACTGTGTTCGCGCGAGGATCACCGCCAGCGCGGTAACCAGACCTAACGTGAGATAAATAAACCAGT‑CGGTTGCAAAAAAGAGCGTATTg                                                                                                                                    <  2:3135890/150‑1 (MQ=255)
                     ggCAGCAACCCCGTGGCGATTAACGTGAACAACTTTTGAATAGAGTCGATTAAAAGTGACTGTGTTCGCGCGAGGATCACCCCCCGCGCGGTAACCCGACCGAACCTGAGATAAAAAAACCAGG‑CGGTTGCAAAAAAGAGCGTATTGaaa                                                                                                                                 >  2:4374568/1‑150 (MQ=255)
                       cagcaACCCCGTGGCGATTAACGTGAACAACTTTTGAATAGAGTCGAGTAAACGTGACAGTGGTCGCGCGCGGATCACCGCCCGCGCGGTAACCACACCAAACGGGGGATAAATAAACCAGT‑CGGGTGGCAAAAATAGCGGAGTGaaaaa                                                                                                                               >  1:3977914/1‑150 (MQ=255)
                        agcaACCCCGTGGCGATTAACGTGAACAACTTTTGAATAGAGTCGATTAAACGTGACTGTGTTCGCGCGAGGATCACCGCCAGCGCGGTAACCAGACCTAACGTGAGATAAATAAACCAGT‑CGGTTGCAAAAAAGAGCGTATTGAAAAAc                                                                                                                              <  1:2392459/150‑1 (MQ=255)
                        agcaACCCCGTGGCGATTAACGTGAACAACTTTTGAATAGAGTCGATTAAACGTGACGGTGTTCGCGCGAGGATCACCGCCAGCGCGGTAACCAGACCTAACGTGAGATAAATAAACCAGT‑CGGTTGCAAAAAAGAGCGTATTGAAAAAc                                                                                                                              >  2:726784/1‑150 (MQ=255)
                        agcaACCCCGTGGCGATTAACGTGAACAACTTTTGAATAGAGTCGATTAAACGGGTCGGTGTTCGCGCGATGATCACCCCCAGCGCGGGAAACAGACCAAACCTGAGATAAAAAAACCACTACGGTTG‑AAAAAAAAGCGTATTGaaaaaa                                                                                                                              >  2:1904214/1‑149 (MQ=255)
                           aaCCCCGTGGCGATTAACGTGAACAACTTTTGAATAGAGTCGATTAAACGTGACTGTGTTCGCGCGAGGATCACCGCCAGCGCGGTAACCAGACCTAACGTGAGATAAATAAACCAGT‑CGGTTGCAAAAAAGAGCGTATTGAAAAACGTg                                                                                                                           >  2:2585572/1‑150 (MQ=255)
                           aaCCCCGTGGCGATTAACGTGAACAACTTTTGAATAGAGTCGATTAAAAGTGACTGTGTTCGCGCGAGGATCACCGCCAGCGCCGTAAACAGAACAAAAGTGAGATAAATAAAACAGT‑CGGTTGCAAAAAAGAGCGTATTGTAAAAAGTg                                                                                                                           >  2:2381154/1‑150 (MQ=255)
                            aCCCCGTGGCGATTAACGTGAACAACTTTTGAATAGAGTCGATTAAACGTGACTGTGTTCGCGCGAGGATCACCGCCAGCGCGGTAACCAGACCTAACGTGAGATAAATAAACCAGT‑CGGTTGCAAAAAAGAGCGTATTGAAAAACGTGa                                                                                                                          <  2:871417/150‑1 (MQ=255)
                            aCCCCGTGGCGATTAACGTGAACAACTTTTGAATAGAGTCGATTAAACGTGACTGTGTTCGCGCGAGGATCACCGCCAGCGCGGTAACCAGACCTAACGTGAGATAAATAAACCAGT‑CGGTTGCAAAAAAGAGCGTATTGAAAAACGTGa                                                                                                                          <  1:1549138/150‑1 (MQ=255)
                             ccccGTGGCGATTAACGTGAACAACTTTTGAATAGAGTCGATTAAACGTGACTGTGTTCGCGCGAGGATCACCGCCAGCGCGGTAACCAGACCTAACGTGAGATAAATAAACCAGT‑CGGTTGCAAAAAAGAGCGTATTGAAAAACGTGAt                                                                                                                         <  2:213223/150‑1 (MQ=255)
                             ccccGTGGCGATTAACGTGAACAACTTTTGAATAGAGTCGATTAAACGTGACTGTGTTCGCGCGAGGATCACCGCCAGCGCGGTAACCAGACCTAACGTGAGATAAATAAACCAGT‑CGGTTGCAAAAAAGAGCGTATTGAAAAACGTGAt                                                                                                                         <  2:1956969/150‑1 (MQ=255)
                             ccccGTGGCGATTAACGTGAACAACTTTTGAATAGAGTCGATTAAACGTGACTGTGTTCGCGCGAGGATCACCGCCAGCGCGGTAACCAGACCTAACGTGAGATAAATAAACCAGT‑CGGTTGCAAAAAAGAGCGTATTGAAAAACGTGAt                                                                                                                         <  2:3330857/150‑1 (MQ=255)
                             ccccGTGGCGATTAACGTGAACAACTTTTGAATAGAGTCGATTAAACGTGACTGTGTTCGCGCGAGGATCACCGCCAGCGCGGTAACCAGACCTAACGTGAGATAAATAAACCAGT‑CGGTTGCAAAAAAGAGCGTATTGAAAAACGTGAt                                                                                                                         <  2:2735172/150‑1 (MQ=255)
                             ccccGTGGCGATTAACGTGAACAACTTTTGAATAGAGTCGATTAAACGTGACTGTGTTCGCGCGAGGATCACCGCCAGCGCGGTAACCAGACCGAACGTGAGATAAATAAAACAGT‑CGGTTGCAAAAAAGAGCGTATTGAAAAACGTGAt                                                                                                                         >  2:2630092/1‑150 (MQ=255)
                              cccGTGGCGATTAACGTGAACAACTTTTGAATAGAGTCGAGTAAAAGTGACTGTGTTCGCGCGAGGATAACCCCCAGCGCGGTAAACAGACCTAAAGTGAGATAAATAAAAAACT‑CGGTTTGAAAAAAAAGAGTATTTTAAAAACTGATg                                                                                                                        >  1:4429330/1‑150 (MQ=255)
                               ccGTGGCGATTAACGTGAACAACTTTTGAATAGAGTCGATTAAACGTGACTGTGTTCGCGCGAGGATCACCGCCAGCGCGGTAACCAGACCTAACGTGAGATAAATAAACCAGT‑CGGTTGCAAAAAAGAGCGTATTGAAAAACGTGATGc                                                                                                                       <  2:899306/150‑1 (MQ=255)
                                 gTGGCGATTAACGTGAACAACTTTTGAATAGAGTCGAGTAAAAGTGACAGTGTTCGCGCGAGGATCACCGCCCGCGCGGTAAACAGACCGAACGTGAGATAAATAAACCAGT‑CGGGTGCAAAAAAGAGCGTATTGAAAAAACTGATGCca                                                                                                                     >  1:2190031/1‑150 (MQ=255)
                                    gCGATTAACGTGAACAACTTTTGAATATAGTCTATTAAACGTGACTGTGTTCGCGCGAGGATAACCGCCAGCGCGGTAACCAGACCTAACGTGAGATAAATAAACCAGT‑CGGTTGCAAAAAAGAGCGTATTGAAAAACGTGATGCcaaca                                                                                                                  <  1:279688/150‑1 (MQ=255)
                                    gCGATTAACGTGAACAACTTTTGAATAGAGTCGATTAAACGTGACTGTGTTCGCGCGAGGATCACCGCCAGCGCGGTAACCAGACCTAACGTGAGATAAATAAACCAGT‑CGGTTGCAAAAAAGAGCGTATTGAAAAACGTGATGCcaaca                                                                                                                  <  1:2544686/150‑1 (MQ=255)
                                         tAACGTGAACAACTTTTGAATAGAGTCGATTAAACGTGACTGTGTTCGCGCGCGGATCACCGCCAGCGCGGTAACCAGACCTAACGTGAGATAAATAAACCAGT‑CGGTTGCAAAAAAGAGCGTATTGAAAAACGTGATGCCAACAAGttt                                                                                                             <  1:465691/150‑1 (MQ=255)
                                            cGTGAACAACTTTTGAATAGAGTCGATTAAACGTGACTGTGTTCGCGCGAGGATCACCGCCAGCGCGGTAACCAGACCTAACGTGAGATAAATAAACCAGT‑CGGTTGCAAAAAAGAGCGTATTGAAAAACGTGATGCCAACAAGTTTaaa                                                                                                          <  2:541/150‑1 (MQ=255)
                                                aacaacTTTTGAATAGAGTCGATTAAACGTGACTGTTTTCGCGCGAGGATCACCGCCAGCGCGGTAACCACACCAAAACTGAGATAAAAAAAACAGT‑CGGTTTCAAAAAATAGCGTATTGAAAAACGTGATGCCAACAAGTTTAaaaaac                                                                                                      >  2:3983132/1‑150 (MQ=255)
                                                  caacTTTTGAATAGAGTCGATTAAACGTGACTGTGTTCGCGCCAGGATAACCGCCACCGCGGTAACCAGACCAAACCTGAGAAAAATAAACCACT‑CGGTTGCCAAAAAGAGCGGAATGAAAAAAGTGATGCCAACAACTTTAaacaacac                                                                                                    >  2:2479218/1‑148 (MQ=255)
                                                  caacTTTTGAATAGAGTCGATTAAAAGTGACAGTTTTAGCGCGAGGATAACCGCCAGCGCGGTAACCAGACCAAACGTGATATAAATAAACCAGT‑AGGTTTCAAAAAATAGCGTATTTAAAAAAAGTATTCCAACAAGTTTAaaaaacac                                                                                                    >  2:2858616/1‑148 (MQ=255)
                                                   aacTTTTTAATAGAGTCGATTAAACGTGAGTGTTTTCGCGCGAGGATCACCGCCTGCGCGGTAACAAGACCTAAGGTGATATAAATAAACCAGT‑CGGTTGCAAAAAAGAGCGTATTGAAAAACGTGATGCAAACAAGTTTAAACAactca                                                                                                   <  1:2403892/150‑1 (MQ=255)
                                                       ttttAATAGTGTCTATTAAAGGTGACTGTGTTCGCGCGAGGATCACCGCCTGCGCGTTAACAATACCTAACGTTATAAAAATAAACCAGT‑CGTTTGAAAAAAAGTGCGTATTGAAAAACGTGATGCCAACAAGTTTAAACAACTCACTcc                                                                                               <  1:2027391/150‑1 (MQ=255)
                                                       tttGAATAGAGTCGATTAAAAGTTACAGTTTTCGCGCGAGGAAAACCGCCAGCGCGGTAAACAGAACAAAAGTGAGATAAATAAAACAGT‑CGGTTTCAAAAAAGAGCGTATTGAAAAAAGTGATTCCAACAAGTTTAAAAAACTAACAcc                                                                                               >  2:3397052/1‑150 (MQ=255)
                                                              agagTCGATTAAACGTGACTGTGTTCGCGCGAGGATCACCGCCAGCGCGGTAACCAGACCTAACGTGAGATAAATAAACCAGG‑CGGTTGCAAAAAAGAGCGTATTGAAAAACGTGATGCCACAAAGTTTAAACAACTCACTCCACAGTaa                                                                                        <  2:3367445/150‑1 (MQ=255)
                                                                agTCGATTAAACGTGACTGTGTTCGCGCGAGGATCACCGCCAGCGCGGTAACCAGACCTAACGTGAGATAAATAAACCAGT‑CGGTTGCAAAAAAGAGCGTATTGAAAAACGTGATGCCAACAAGTTTAAACAACTCACTCCACAGTaaaa                                                                                      <  2:4097086/150‑1 (MQ=255)
                                                                   cGATTAAACGTGACTGTGTTCGCGCGAGGATCACCGCCAGCGCGGTAACCAGACCTAACGTGAGATAAATAAACCAGT‑CGGTTGCAAAAAAGAGCGTATTGAAAAACGTGATGCCAACAAGTTTAAACAACTCACTCCACAGTAAAAGCa                                                                                   >  2:358805/1‑150 (MQ=255)
                                                                   cGATTAAACGTGACTGTGTTCGCGCGAGGATCACCGCCAGCGCGGTAACCAGACCTAACGTGAGATAAATAAACCAGT‑CGGTTGCAAAAAAGAGCGTATTGAAAAACGTGATGCCAACAAGTTTAAACAACTCACTCCACAGTAAAAGCa                                                                                   >  2:2717837/1‑150 (MQ=255)
                                                                    gtttAAACGTGTCTGTGTTCGCGCGAGTATCACCGCCAGCGCGTTAACCAGACCTAACGTTATATAAATAAACCAGT‑CGGTTGCAAAAAAGAGCGTATTTAAAAACGTGATGCCAACAAGTTTAAACAACTCACTCCACAGTAAAAGCAc                                                                                  <  1:3585253/148‑1 (MQ=255)
                                                                    gATTAAACGTGACTGTGTTCGCGCGAGGATCACCGCCAGCGCGGTAACCAGACCTAACGTGAGATAAATAAACCAGT‑CGGTTGCAAAAAAGAGCGTATTGAAAAACGTGATGCCAACAAGTTTAAACAACTCACTCCACAGTAAAAGCAc                                                                                  >  2:1248702/1‑150 (MQ=255)
                                                                     aTTAAACGTGACTGTGTTCGCGCGAGGATCACCGCCAGCGCGGTAACCAGACCTAACGTGAGATAAATAAACCAGT‑CGGTTGCAAAAAAGAGCGTATTGAAAAACGTGATGCCAACAAGTTTAAACAACTCACTCCACAGTAAAAGCAcc                                                                                 >  1:1241285/1‑150 (MQ=255)
                                                                      ttAAACGTGACTGTGTTCGCGCGAGGATCACCGCCAGCGCGGTAACCAGACCTAACGTGAGATAAATAAACCAGT‑CGGTTGCAAAAAAGAGCGTATTGAAAAACGTGATGCCAACAAGTTTAAACAACTCACTCCACAGTAAAAGCACCa                                                                                >  2:2198135/1‑150 (MQ=255)
                                                                        aaaCGTGACTGTGTTCGCGCGAGGATCACCGCCAGCGCGGTAACCAGACCTAACGTGAGATAAATAAACCAGT‑CGGTTGCAAAAAAGAGCGTATTGAAAAACGTGATGCCAACAAGTTTAAACAACTCACTCCACAGTAAAAGCACCAGc                                                                              >  2:809353/1‑150 (MQ=255)
                                                                         aaCGTGACTGTGTTCGCGCGAGGATCACCGCCAGCGCGGTAACCAGACCTAACGTGAGATAAATAAACCAGT‑CGGGTGCAAAAAAGAGCGTATTGAAAAACGTGATGCCAACAAGTTTAAACAACTCACTCCACAGTAAAAGCACCAGcc                                                                             >  1:4217088/1‑150 (MQ=255)
                                                                          aCGTGACTGTGTTCGCGCGAGGATCACCGCCAGCGCGGTAACCAGACCTAACGTGAGATAAATAAACCAGT‑CGGTTGCAAAAAAGAGCGTATTGAAAAACGTGATGCCAACAAGTTTAAACAACTCACTCCACAGTAAAAGCACCAGCCa                                                                            <  2:2938723/150‑1 (MQ=255)
                                                                           cGTGACTGTGTTCGCGCGAGGATCACCGCCAGCGCGGTAACCAGACCTAACGTGAGATAAATAAACCAGT‑CGGTTGCAAAAAAGAGCGTATTGAAAAACGTGATGCCAACAAGTTTAAACAACTCACTCCACAGTAAAAGCACCAGCCAc                                                                           <  2:2082783/150‑1 (MQ=255)
                                                                           cGTGACTGTGTTCGCGCGAGGATCACCGCCAGCGCGGTAACCAGACCTAACGTGAGATAAATAAACCAGT‑CGGTTGCAAAAAAGAGCGTATTGAAAAACGTGATGCCAACAAGTTTAAACAACTCACTCCACAGTAAAAGCACCAGCCAc                                                                           <  2:4083157/150‑1 (MQ=255)
                                                                                 tgtgTTCGCGCGAGGATCACCGCCAGCGCGGTAACCAGACCTAACGTGAGATAAATAAACCAGT‑CGGTTGCAAAAAAGAGCGTATTGAAAAACGTGATGCCAACAAGTTTAAACAACTCACTCCACAGTAAAAGCACCAGCCACGTCAAg                                                                     >  2:134745/1‑150 (MQ=255)
                                                                                       cgcgcgAGGATCACCGCCAGCGCGGTAACCAGACCTAACGTGAGATAAATAAACCAGT‑CGGTTGCAAAAAAGAGCGTATTGAAAAACGTGATGCCAACAAGTTTAAACAACTCACTCCACAGTAAAAGCACCAGCCACGTCAAGCCATTg                                                               >  2:144950/1‑150 (MQ=255)
                                                                                        gcgcgAGGATCACCGCCAGCGCGGTAACCAGACCTAACGTGAGATAAATAAACCAGT‑CGGTTGCAAAAAAGAGCGTATTGAAAAACGTGATGCCAACAAGTTTAAACAACTCACTCCACAGTAAAAGCACCAGCCACGTCAAGCCATTgg                                                              <  1:3289965/150‑1 (MQ=255)
                                                                                         cgcgTGGATCACCGCCAGCGCGGTAACCAGACCTAACGTGAGATAAATAAACCAGT‑CGGTTGCAAAAAAGAGCGTATTGAAAAACGTGATGCCAACACGTTTAAACAACTCACTCCACAGTAAAAGCACCAGCCACGTCAAGCCATTGGc                                                             <  2:3029187/150‑1 (MQ=255)
                                                                                         cgcgAGGATCACCTCCAGCGCGGTAACCAGACCTAACGTGAGATAAATAAACCAGG‑CGGTTGCAAAAAAGAGCGTATTGAAAAACGTGATGCCAACAAGTTTAAACAACTCACTCCACAGTAAAAGCACCAGCCACGTCAAGCCATTGGc                                                             <  2:3260458/150‑1 (MQ=255)
                                                                                         cgcgAGGATCACCGCCAGCGCGGTAACCAGACATAACGTTATATAAATAAACCAGT‑CGGTTGCAAAAAAGAGCGTATTGAAAAACGTGATGCCAACAAGTTTAAACAACTCACTCCACAGTAAAAGCACCAGCCACGTCAAGCCATTGGc                                                             <  1:4200628/150‑1 (MQ=255)
                                                                                             aGGATCACCGCCAGCGCGGTAACCAGACCTAACGTGAGATAAATAAACCAGT‑CGGTTGCAAAAAAGAGCGTATTGAAAAACGTGATGCCAACAAGTTTAAACAACTCACTCCACAGTAAAAGCACCAGCCACGTCAAGCCATTGGCgaga                                                         >  1:3723658/1‑150 (MQ=255)
                                                                                              gcaTCACCGCCAGCGCGTAAACCAGACCTAACGTGACATAAAAAAACCAGT‑CGGTTGCAAAAAAGAGCGTATTGAAAAACGTGATGCCAACAAGTTTAAACAACTCACTCCACAGTAAAAGCACCAGCCACGTCAAGCCATTGGCGAGaa                                                        <  1:1970523/148‑1 (MQ=255)
                                                                                               gaacACCGCCTGCGCGGTAACCAGACATAACGTTATATAAATAAACCCGT‑CGGTTGCAAAAAAGAGCGTATTGAAAAACGTGATGCCAACAAGTTTAAACAACTCACTCCACAGTAAAAGCACCAGCCACGTCAAGCCATTGGCGAGaaa                                                       <  1:3117033/147‑1 (MQ=255)
                                                                                                  cACCGCCAGCGCGTAAACAATACCTAACTTTATAAAAATAAACCAGT‑CGTTTGCAAAAAAGAGCTTTTTGAAAAACGTGATGCCAACAAGTTTAAACAACTCACTCCACAGTAAAAGCACCAGCCACGTCAAGCCATTGGCGAGAAAAAt                                                    <  1:818693/150‑1 (MQ=255)
                                                                                                   aCCGCCCGCGCGGTAACCAGACCTAACGTGAGATAAATAAACCAGT‑CGGTTGCAAAAAAGAGCGTATTGAAAAACGTGATGCCAACAAGTTTAAACAACTCACTCCACAGTAAAAGCACCAGCCACGTCAAGCCATTGGCGAGAAAAATc                                                   <  1:1975826/150‑1 (MQ=255)
                                                                                                   aCCGCCAGCGCGGTAACCAGACCTAACGTGAGAAAAATAAACCAGT‑CGGTTGCAAAAAAGAGCGTATTGAAAAACGTGATGCCAACAAGTTTAAACAACTCACTCCACAGTAAAAGCACCAGCCACGTCAAGCCATTGGCGAGAAAAATc                                                   <  1:1976317/150‑1 (MQ=255)
                                                                                                      gCCAGCGCGGTAACCAGACCTAACGTGAGATAAATAAACCAGT‑CGGTTGCAAAAAAGAGCGTATTGAAAAACGTGATGCCAACAAGTTTAAACAACTCACTCCACAGTAAAAGCACCAGCCACGTCAAGCCATTGGCGAGAAAAATCAcc                                                <  2:3099348/150‑1 (MQ=255)
                                                                                                        cAGCGCGGTAACCAGACCTAACGTGAGATAAATAAACCAGT‑CGGTTGCAAAAAAGAGCGTATTGAAAAACGTGATGCCAACAAGTTTAAACAACTCACTCCACAGTAAAAGCACCAGCCACGTCAAGCCATTGGCGAGAAAAATCACCAg                                              >  1:4442175/1‑150 (MQ=255)
                                                                                                        cAGCGCGGTAACCAGACCTAACGTGAGATAAATAAACCAGT‑CGGTTGCAAAAAAGAGCGTATTGAAAAACGTGATGCCAACAAGTTTAAACAACTCACTCCACAGTAAAAGCACCAGCCACGTCAAGCCATTGGCGAGAAAAATCACCAg                                              >  1:852823/1‑150 (MQ=255)
                                                                                                           cgcgGTAACCAGACCTAACGTGAGATAAATAAACCAGT‑CGGTTGCAAAAAAGAGCGTATTGAAAAACGTGATGCCAACAAGTTTAAACAACTCACTCCACAGTAAAAGCACCAGCCACGTCAAGCCATTGGCGAGAAAAATCACCAGTaa                                           <  1:1359593/150‑1 (MQ=255)
                                                                                                           cgcgGTAACCAGACCTAACGTGAGATAAATAAACCAGT‑CGGTTGCAAAAAAGAGCGTATTGAAAAACGTGATGCCAACAAGTTTAAACAACTCACTCCACAGTAAAAGCACCAGCCACGTCAAGCCATTGGCGAGAAAAATCACCAGTaa                                           >  1:3460630/1‑150 (MQ=255)
                                                                                                            gcgGTAACCAGACCTAACGTGAGATAAATAAACCAGT‑CGGTTGCAAAAAAGAGCGTATTGAAAAACGTGATGCCAACAAGTTTAAACAACTCACTCCACAGTAAAAGCACCAGCCACGTCAAGCCATTGGCGAGAAAAATCACCAGTAat                                          <  2:1722253/150‑1 (MQ=255)
                                                                                                                tAACCAGACCTAACGTGATATAAATAAACCAGT‑CGGTTGAAAAAAAGAGCGTATTGAAAAACGTGATGCCAACAAGTTTAAACAACTCACTCCACAGTAAAAGCACCAGCCACGTCAAGCCATTGGCGAGAAAAATCACCAGTAATATaa                                      <  1:99817/150‑1 (MQ=255)
                                                                                                                tAACCAGACCTAACGTGAGATAAATAAACCAGT‑CGGTTGCAAAAAAGAGCGTATTGAAAAACGTGATGCCAACAAGTTTAAACAACTCACTCCACAGTAAAAGCACCAGCCACGTCAAGCCATTGGCGAGAAAAATCACCAGTAATATaa                                      >  2:937154/1‑150 (MQ=255)
                                                                                                                    cAGACCTAACGTGAGATAAATAAACCAGT‑CGGTTGCAAAAAAGAGCGTATTGAAAAACGTGATGCCAACAAGTTTAAACAACTCACTCCACAGTAAAAGCACCAGCCACGTCAAGCCATTGGCGAGAAAAATCACCAGTAATATAAGTAc                                  <  2:2590397/150‑1 (MQ=255)
                                                                                                                        catAACGTGAGATAAATAAACCAGT‑CGGTTGCAAAAAAGAGCGTATTGAAAAACGTGATGCCAACAAGTTTAAACAACTCACTCCACAGTAAAAGCACCAGCCACGTCAAGCCATTGGCGAGAAAAATCACCAGTAATATAAGTACGTTa                              <  1:2610285/148‑1 (MQ=255)
                                                                                                                        catAACGTGAGATAAATAAACCAGT‑CGGTTGCAAAAAAGAGCGTATTGAAAAACGTGATGCCAACAAGTTTAAACAACTCACTCCACAGTAAAAGCACCAGCCACGTCAAGCCATTGGCGAGAAAAATCACCAGTAATATAAGTACGTTa                              <  1:4253030/148‑1 (MQ=255)
                                                                                                                         cTAACGTGAGATAAATAAACCAGT‑CGGTTGCAAAAAAGAGCGTATTGAAAAACGTGATGCCAACAAGTTTAAACAACTCACTCCACAGTAAAAGCACCAGCCACGTCAAGCCATTGGCGAGAAAAATCACCAGTAATATAAGTACGTTAt                             <  2:2889574/150‑1 (MQ=255)
                                                                                                                          tAACGTGAGATAAATAAACCAGT‑CGGTTGCAAAAAAGAGCGTATTGAAAAACGTGATGCCAACAAGTTTAAACAACTCACTCCACAGTAAAAGCACCAGCCACGTCAAGCCATTGGCGAGAAAAATCACCAGTAATATAAGTACGTTATg                            <  1:720620/150‑1 (MQ=255)
                                                                                                                                gagaTAAATAAACCAGT‑CGGTTGCAAAAAAGAGCGTATTGAAAAACGTGATGCCAACAAGTTTAAACAACTCACTCCACAGTAAAAGCACCAGCCACGTCAAGCCATTGGCGAGAAAAATCACCAGTAATATAAGTACGTTATGCCATac                      >  2:164747/1‑150 (MQ=255)
                                                                                                                                 agaTAAATAAACCAGT‑CGGTTGCAAAAAAGAGCGTATTGAAAAACGTGATGCCAACAAGTTTAAACAACTCACTCCACAGTAAAAGCACCAGCCACGTCAAGCCATTGGCGAGAAAAATCACCAGTAATATAAGTACGTTATGCCATact                     >  2:3189454/1‑150 (MQ=255)
                                                                                                                                 agaTAAATAAACCAGT‑CGGTTGCAAAAAAGAGCGTATTGAAAAACGTGATGCCAACAAGTTTAAACAACTCACTCCACAGTAAAAGCACCAGCCACGTCAAGCCATTGGCGAGAAAAATCACCAGTAATATAAGTACGTTATGCCATact                     <  2:646329/150‑1 (MQ=255)
                                                                                                                                 agaTAAATAAACCAGT‑CGGTTGCAAAAAAGAGCGTATTGAAAAACGTGATGCCAACAAGTTTAAACAACTCACTCCACAGTAAAAGCACCAGCCACGTCAAGCCATTGGCGAGAAAAATCACCAGTAATATAAGTACGTTATGCCATact                     >  2:3189545/1‑150 (MQ=255)
                                                                                                                                  gaTAAATAAACCAGT‑CGGTTGCAAAAAAGAGCGTATTGAAAAACGTGATGCCAACAAGTTTAAACAACTCACTCCACAGTAAAAGCACCAGCCACGTCAAGCCATTGGCGAGAAAAATCACCAGTAATATAAGTACGTTATGCCATactg                    <  1:198881/150‑1 (MQ=255)
                                                                                                                                   ataaataaACCAGT‑CGGTTGCAAAAAAGAGCGTATTGAAAAACGTGATGCCAACAAGTTTAAACAACTCACTCCACAGTAAAAGCACCAGCCACGTCAAGCCATTGGCGAGAAAAATCACCAGTAATATAAGTACGTTATGCCATactga                   <  2:4281728/150‑1 (MQ=255)
                                                                                                                                    taaataaaCCAGT‑CGGTTGCAAAAAAGTGCGTATTGAAAAACGTGATGCCAACAAGTTTAAACAACTCACTCCACAGTAAAAGCACCAGCCACGTCAAGCCATTGGCGAGAAAAATCACCAGTAATATAAGTACGTTATGCCATactgac                  >  1:3769056/1‑150 (MQ=255)
                                                                                                                                      aataaaCCAGT‑CGGTTGCAAAAAAGAGCGTATTGAAAAACGTGATGCCAACAAGTTTAAACAACTCACTCCACAGTAAAAGCACCAGCCACGTCAAGCCATTGGCGAGAAAAATCACCAGTAATATAAGTACGTTATGCCATactgactg                <  1:4462941/150‑1 (MQ=255)
                                                                                                                                        tacaCCCGT‑CGGTTGCAAAAAAGAGCGTATTGAAAAACGTGATGCCACCAAGTCTAAACAACTCACTCCACACAAAAAGCACCACCCACGTCACGCCATCGGCGAGAACCATCACCAGTAATCTACGTACGTTATGCCATACTGCCTGGt              <  2:1084863/147‑1 (MQ=255)
                                                                                                                                         aaaCCAGT‑CGGTTGCAAAAAAGAGCGTATTGAAAAACGTGATGCCAACAAGTTTAAACAACTCACTCCACAGTAAAAGCACCAGCCACGTCAAGCCATTGGCGAGAAAAATCACCAGTAATATAAGTACGTTATGCCATACTGACTGGTa             <  1:2185526/150‑1 (MQ=255)
                                                                                                                                              aGT‑CGTTTGAAAAAAATAGCTTATTGAAAAACGTGATGCCAACAAGTTTAAACAACTCACTCCACAGTAAAAGCACCAGCCACGTCAAGCCATTGGCGAGAAACATCACCAGTAATATAAGTACGTTATGCCATACTGACTGGTAAAAat        <  1:4048338/150‑1 (MQ=255)
                                                                                                                                              aGT‑CGGTTGCAAAAAAGAGCGTATTGAAAAACGTGATGCCAACAAGTTTAAACAACTCACTCCACAGTAAAAGCACCAGCCACGTCAAGCCATTGGCGAGAAAAATCACCAGTAATATAAGTACGTTATGCCATACTGACTGGTAAAAat        <  1:518117/150‑1 (MQ=255)
                                                                                                                                                    gTTGCAAAAAAGAGCGTATTGAAAAACGTGATGCCAACAAGTTTAAACAACTCACTCCACAGTAAAAGCACCAGCCACGTCAAGCCATTGGCGAGAAAAATCACCAGTAATATAAGTACGTTATGCCATACTGACTGGTAAAAatagcga   >  2:2844066/1‑150 (MQ=255)
                                                                                                                                                     ttGCAAAAAAGAGCTTATTGAAAAACGTGATGCCAACACGTTTAAACAACTCACTCCACAGTAAAAGCACCAGCCACGTCAAGCCATTGGCGAGAAAAATCACCAGTAATATAAGTACGTTATGCCATACTGACTGGTAAAAatagcgat  <  2:45763/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
AGGGTTAGCAATGATACTAACGGCAGCAACCCCGTGGCGATTAACGTGAACAACTTTTGAATAGAGTCGATTAAACGTGACTGTGTTCGCGCGAGGATCACCGCCAGCGCGGTAACCAGACCTAACGTGAGATAAATAAACCAGT‑CGGTTGCAAAAAAGAGCGTATTGAAAAACGTGATGCCAACAAGTTTAAACAACTCACTCCACAGTAAAAGCACCAGCCACGTCAAGCCATTGGCGAGAAAAATCACCAGTAATATAAGTACGTTATGCCATACTGACTGGTAAAAATAGCGAT  >  CP009273/3525110‑3525407

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑