BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	3,551,373	T→G	9.6%	V22V (GTA→GTC)	rtcB ←	RNA‑splicing ligase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	3,551,373	0	T	G	9.6%	882.0 / 82.2	306	V22V (GTA→GTC)	rtcB	RNA‑splicing ligase
Reads supporting (aligned to +/- strand): ref base T (124/152); new base G (14/16); total (138/168)
Fisher's exact test for biased strand distribution p-value = 8.50e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.49e-01

Only 100 of 313 total aligned reads displayed.

ACCGCCGCCGGAATAATCGCCCCTTTGGTCGGGATCACGCTACCAATGGTGGAACCTTTACCCAGGTGTACATCAGGCATTACCGCAATATGTTTGAAAATAAACGGCATCTTCGCCGTATTAATAAGTTGCTGACGCGCATCGGCCTCTACCGGCACGCCTTTGGTCCACATTTTTACCGGGGCATTTTCAGTGGTCAGTAATTCGTAATTCATTTTGTTTTTCTCTTTTCGTTGTTTGCTGTCCTGATAATTGCAACCGTCGTGCCAGAAAATTAAATAAGCAGCTTAATTTTTTAA  >  CP009273/3551224‑3551522

 ccgccgccgGAATAATCGCCCCTTTGGTCGGGATCACGCTACCAATGGTGGAACCTTTACCCAGGTGTACATCAGGCATTACCGCAATATGTTTGAAAATAAACGGCATCTTCGCCGTATTAATAAGTTGCTGACGCGCATCGGCCTCTa                                                                                                                                                      <  2:4403176/150‑1 (MQ=255)

     cgccgGAATAATCGCCCCTTTGGTCGGGATCACGCTACCAATGGTGGAACCTTTACCCAGGTGTACATCAGGCATTACCGCAATATGTTTGAAAATAAACGGCATCTTCGCCGTATTAATAAGTTGCTGACGCGCATCGGCCTCTACCgg                                                                                                                                                  >  2:4201713/1‑150 (MQ=255)

          gAATAATCGCCCCTTTGGTCGGGATCACGCTACCAATGGTGGAACCTTTACCCAGGTGTACATCAGGCATTACCGCAATATGTTTGAAAATAAACGGCATCTTCGCCGTATTAATAAGTTGCTGACGCGCATCGGCCTCTACCGGCACGc                                                                                                                                             >  2:509690/1‑150 (MQ=255)

              actCGCCCCTTGGGTCGGGATCACGCTACCAATGGTGGAACCTTTACCCAGGTGTACCTCAGGCAGTACCGCAATCTGTTTGAAAATAAACGGCATCTTCGCCGTATTAATAAGTTGCTGCCGCGCATCGGCCTCTCCCGGCACGCCttt                                                                                                                                         <  2:4147960/148‑1 (MQ=255)

              aatCGCCCCTTTGGTCGGGATCACGCTACCAATGGTGGAACCTTTACCCAGGTGTACATCAGGCATTACCGCAATATGTTTGAAAATAAACGGCATCTTCGCCGTATTAATAAGTTGCTGACGCGCATCGGCCTCTACCGGCACGCCttt                                                                                                                                         <  2:2956423/150‑1 (MQ=255)

              aatCGCCCCTTTGGTCGGGATCACGCTACCAATGGTGGAACCTTTACCCAGGTGTACATCAGGCATTACCGCAATATGTTTGAAAATAAACGGCATCTTCGCCGTATTAATAAGTTGCTGACGCGCATCGGCCTCTACCGGCACGCCttt                                                                                                                                         >  1:1814034/1‑150 (MQ=255)

               atCGCCCCTTTGGTCGGGATCACGCTACCAATGGTGGAACCTTTACCCAGGTGTACATCAGGCATTACCGCAATATGTTTGAAAATAAACGGCATCTTCGCCGTATTAATAAGTTGCTGACGCGCATCGGCCTCTACCGGCACGCCTTTg                                                                                                                                        <  2:2893192/150‑1 (MQ=255)

               atCGCCCCTTTGGTCGGGATCACGCTACCAATGGTGGAACCTTTACCCAGGTGTACATCAGGCATTACCGCAATATGTTTGAAAATAAACGGCATCTTCGCCGTATTAATAAGTTGCTGACGCGCATCGGCCTCTACCGGCACGCCTTTg                                                                                                                                        >  1:2378975/1‑150 (MQ=255)

                tCGCCCCTTTGGTCGGGATCACGCTACCAATGGTGGAACCTTTACCCAGGTGTACATCAGGCATTACCGCAATATGTTTGAAAATAAACGGCATCTTCGCCGTATTAATAAGTTGCTGACGCGCATCGGCCTCTACCGGCACGCCTTTgg                                                                                                                                       <  2:3880731/150‑1 (MQ=255)

                 cGCCCCTTTGGTCGGGATCACGCTACCAATGGTGGAACCTTTACCCAGGTGTACATCAGGCATTACCGCAATATGTTTGAAAATAAACGGCATCTTCGCCGTATTAATAAGTTGCTGACGCGCATCGGCCTCTACCGGCACGCCTTTGGt                                                                                                                                      <  1:319747/150‑1 (MQ=255)

                   ccccTTTGGTCGGGATCACGCTACCAATGGTGGAACCTTTACCCAGGTGTACATCAGGCATTACCGCAATATGTTTGAAAATAAACGGCATCTTCGCCGTATTAATAAGTTGCTGACGCGCATCGGCCTCTACCGGCACGCCTTTGGTcc                                                                                                                                    >  2:3929125/1‑150 (MQ=255)

                     ccTTTGGTCGGGATCACGCTACCAATGGTGGAACCTTTACCCAGGTGTACATCAGGCATTACCGCAATATGTTTGAAAATAAACGGCATCTTCGCCGTATTAATAAGTTGCTGACGCGCATCGGCCTCTACCGGCACGCCTTTGGTCcac                                                                                                                                  >  1:3025432/1‑150 (MQ=255)

                      cTTTGGTCGGGATCACGCTACCACTGGTGGAACCTTTACCCAGGTGTACATCAGGCATTACCGCAATATGTTTGAAAATAAACGGCATCTTCGCCGTATTAATAAGTTGCTGACGCGCATCGGCCTCTACCGGCACGCCTTTGGTCcaca                                                                                                                                 <  1:1585410/150‑1 (MQ=255)

                        ttGGTCGGGATCACGCTACCAATGGTGGAACCTTTACCCAGGTGTACATCAGGCATTACCGCAATATGTTTGAAAATAAACGGCATCTTCGCCGTATTAATAAGTTGCTGACGCGCATCGGCCTCTACCGGCACGCCTTTGGTCCACAtt                                                                                                                               >  2:3202857/1‑150 (MQ=255)

                            tCGGGATCACGCTACCAATGGTGGAACCTTTACCCAGGTGTACATCAGGCATTACCGCAATATGTTTGAAAATAAACGGCATCTTCGCCGTATTAATAAGTTGCTGACGCGCATCGGCCTCTACCGGCACGCCTTTGGTCCTCATTTTTa                                                                                                                           <  2:754816/150‑1 (MQ=255)

                              gggATCACGCTACCAATGGTGGAACCTTTACCCAGGTGTACATCAGGCATTACCGCAATATGTTTGAAAATAAACGGCATCTTCGCCGTATTAATAAGTTGCTGACGCGCATCGGCCTCTACCGGCACGCCTTTGGTCCACATTTTTAcc                                                                                                                         <  1:3891940/150‑1 (MQ=255)

                               ggATCACGCTACCAATGGTGGAACCTTTACCCAGGTGTACATCAGGCATTACCGCAATATGTTTGAAAATAAACGGCATCTTCGCCGTATTAATAAGTTGCTGACGCGCATCGGCCTCTACCGGCACGCCTTTGGTCCACATTTTTACCg                                                                                                                        >  1:1346296/1‑150 (MQ=255)

                               ggATCACGCTACCAATGGTGGAACCTTTACCCAGGTGTACATCAGGCATTACCGCAATATGTTTGAAAATAAACGGCATCTTCGCCGTATTAATAAGTTGCTGACGCGCATCGGCCTCGACCGGCACGCCTTTGGTCCACATTTTTACCg                                                                                                                        >  1:815470/1‑150 (MQ=255)

                               ggATCACGCTACCAATGGTGGAACCTTTACCCAGGTGTACATCAGGCATTACCGCAATATGTTTGAAAATAAACGGCATCTTCGCCGTATTAATAAGTTGCTGACGCGCATCGGCCTCGACCGGCACGCCTTTGGTCCACATTTTTACCg                                                                                                                        >  1:726201/1‑150 (MQ=255)

                                 aTCACGCTACCAATGGTGGAACCTTTACCCAGGTGTACATCAGGCATTACCGCAATATGTTTGAAAATAAACGGCATCTTCGCCGTATTAATAAGTTGCTGACGCGCATCGGCCTCTACCGGCACGCCTTTGGTCCACATTTTTACCggg                                                                                                                      >  1:532582/1‑150 (MQ=255)

                                  tCACGCTACCAATGGTGGAACCTTTACCCCGGTGTACATCAGGCATTACCGCAATATGTTTGAAAATAAACGGCATCTTCGCCGTATTAATAAGTTGCTGACGCGCATCGGCCTCTACCGGCACGCCTTTGGTCCACATTTTTACCgggg                                                                                                                     <  1:3816656/150‑1 (MQ=255)

                                      gCTACCAATGGTGGAACCTTTACCCAGGTGTACATCAGGCATTACCGCAATATGTTTGAAAATAAACGGCATCTTCGCCGTATTAATAAGTTGCTGACGCGCATCGGCCTCTACCGGCACGCCTTTGGTCCACATTTTTACCGGGGCAtt                                                                                                                 <  1:3182962/150‑1 (MQ=255)

                                        tACCACTGGTGGAACCTTTACCCAGGTGTACATCAGGCATTACCGCAATATGTTTGAAAATAAACGGCATCTTCGCCGTATTAATAAGTTGCTGACGCGCATCGGCCTCTACCGGCACGCCTTTGGTCCACATTTTTACCGGGGCAtttt                                                                                                               <  2:2122155/150‑1 (MQ=255)

                                        tACCAATGGTGGAACCTTTACCCAGGTGTACATCAGGCATTACCGCAATATGTTTGAAAATAAACGGCATCTTCGCCGTATTAATAAGTTGCTGACGCGCATCGGCCTCTACCGGCACGCCTTTGGTCCACATTTTTACCGGGGCAtttt                                                                                                               <  1:2084565/150‑1 (MQ=255)

                                        tACCAATGGTGGAACCTTTACCCAGGTGTACATCAGGCATTACCGCAATATGTTTGAAAATAAACGGCATCTTCGCCGTATTAATAAGTTGCTGACGCGCATCGGCCTCTACCGGCACGCCTTTGGTCCACATTTTTACCGGGGCAtttt                                                                                                               <  2:1476533/150‑1 (MQ=255)

                                         aCCAATGGTGGAACCTTTACCCAGGTGTACATCAGGCATTACCGCAATATGTTTGAAAATAAACGGCATCTTCGCCGTATTAATAAGTTGCTGACGCGCATCGGCCTCTACCGGCACGCCTTTGGTCCACATTTTTACCGGGGCATTTTc                                                                                                              >  1:2014653/1‑150 (MQ=255)

                                          ccAATGGTGGAACCTTTACCCAGGTGTACATCAGGCATTACCGCAATATGTTTGAAAATAAACGGCATCTTCGCCGTATTAATAAGTTGCTGACGCGCATCGGCCTCTACCGGCACGCCTTTGGTCCACATTTTTACCGGGGCATTTTCa                                                                                                             <  2:4301740/150‑1 (MQ=255)

                                          ccAATGGTGGAACCTTTACCCAGGTGTACATCAGGCATTACCGCAATATGTTTGAAAATAAACGGCATCTTCGCCGTATTAATAAGTTGCTGACGCGCATCGGCCTCTACCGGCACGCCTTTGGTCCACATTTTTACCGGGGCATTTTCa                                                                                                             <  2:1548301/150‑1 (MQ=255)

                                          ccAATGGTGGAACCTTTACCCAGGTGTACATCAGGCATTACCGCAATATGTTTGAAAATAAACGGCATCTTCGCCGTATTAATAAGTTGCTGACGCGCATCGGCCTCGACCGGCACGCCTTTGGTCCACATTTTTACCGGGGCATTTTCa                                                                                                             <  2:1417519/150‑1 (MQ=255)

                                           cAATGGTGGAACCTTTACCCAGGTGTACATCAGGCATTACCGCAATATGTTTGAAAATAAACGGCATCTTCGCCGTATTAATAAGTTGCTGACGCGCATCGGCCTCTACCGGCACGCCTTTGGTCCACATTTTTACCGGGGCATTTTCAg                                                                                                            <  2:1119203/150‑1 (MQ=255)

                                                 tggAACCTTTACCCAGGTGTACATCAGGCATTACCGCAATATGTTTGAAAATAAACGGCATCTTCGCCGTATTAATAAGTTGCTGACGCGCATCGGCCTCTACCGGCACGCCTTTGGTCCACATTTTTACCGGGGCATTTTCAGTGGTCa                                                                                                      <  2:4039596/150‑1 (MQ=255)

                                                  ggAACCTTTACCCAGGTGTACATCAGGCATTACCGCAATATGTTTGAAAATAAACGACATCTTCGCCGTATTAATAAGTTGCTGACGCGCATCGGCCTCTACCGGCACGCCTTTGGTCCACATTTTTACCGGGGCATTTTCAGGGGTCAg                                                                                                     >  2:3052687/1‑150 (MQ=255)

                                                    aaCCTTTACCCAGGTGTACATCAGGCATTACCGCAATATGTTTGAAAATAAACGGCATCTTCGCCGTATTAATAAGTTGCTGACGCGCATCGGCCTCTACCGGCACGCCTTTGGTCCACATTTTTACCGGGGCATTTTCAGTGGTCAgta                                                                                                   <  2:3843356/150‑1 (MQ=255)

                                                      ccTTTACCCAGGTGTACATCAGGCATTACCGCAATATGTTTGAAAATAAACGGCATCTTCGCCGTATTAATAAGTTGCTGACGCGCATCGGCCTCTACCGGCACGCCTTTGGTCCACATTTTTACCGTGGCATTTTCAGTGGTCAgtaat                                                                                                 >  2:2813867/1‑150 (MQ=255)

                                                      ccTTTACCCAGGTGTACATCAGGCATTACCGCAATATGTTTGAAAATAAACGGCATCTTCGCCGTATTAATAAGTTGCTGACGCGCATCGGCCTCTACCGGCACGCCTTTGGTCCACATTTTTACCGGGGCATTTTCAGTGGTCAgtaat                                                                                                 >  1:1216640/1‑150 (MQ=255)

                                                      ccTTTACCCAGGTGTACATCAGGCATTACCGCAATATGTTTGAAAATAAACGGCATCTTCGCCGTATTAATAAGTTGCTGACGCGCATCGGCCTCTACCGGCACGCCTTTGGTCCACATTTTTACCGGGGCATTTTCAGTGGTCAgtaat                                                                                                 <  2:1051640/150‑1 (MQ=255)

                                                       cTTTACCCAGGTGTACATCAGGCATTACCGCAATATGTTTGAAAATAAACGGCATCTTCGCCGTATTAATAAGTTGCTGACGCGCATCGGCCTCTACCGGCACGCCTTTGGTCCACATTTTTACCGGGGCATTTTCAGTGGTCAgtaatt                                                                                                >  1:620786/1‑150 (MQ=255)

                                                       cTTTACCCAGGTGTACATCAGGCATTACCGCAATATGTTTGAAAATAAACGGCATCTTCGCCGTATTAATAAGTTGCTGACGCGCATCGGCCTCTACCGGCACGCCTTTGGTCCACATTTTTACCGGGGCATTTTCAGTGGTCAgtaatt                                                                                                >  1:4283762/1‑150 (MQ=255)

                                                       cTTTACCCAGGTGTACATCAGGCATTACCGCAATATGTTTGAAAATAAACGGCATCTTCGCCGTATTAATAAGTTGCTGACGCGCATCGGCCTCTACCGGCACGCCTTTGGTCCACATTTTTACCGGGGCATTTTCAGTGGTCAgtaatt                                                                                                <  2:2676463/150‑1 (MQ=255)

                                                        tttACCCAGGTGTACATCAGGCATTACCGCAATATGTTTGAAAATAAACGGCATCTTCGCCGTATTAATAAGTTGCTGACGCGCATCGGCCTCTACCGGCACGCCTTTGGTCCACATTTTTACCGGGGCATTTTCAGTGGTCAgtaattc                                                                                               <  1:1228521/150‑1 (MQ=255)

                                                         ttACCCAGGTGTACATCAGGCATTACCGCAATATGTTTGAAAATAAACGGCATCTTCGCCGTATTAATAAGTTGCTGACGCGCATCGGCCTCGACCGGCACGCCTTTGGTCCACATTTTTACCGGGGCATTTTCAGTGGTCAgtaattcg                                                                                              >  2:4363929/1‑150 (MQ=255)

                                                          tACCCAGGTGTACATCAGGCATTACCGCAATATGTTTGAAAATAAACGGCATCTTCGCCGTATTAATAAGTTGCTGACGCGCATCGGCCTCTACCGGCACGCCTTTGGTCCACATTTTTACCGGGGCATTTTCAGTGGTCAgtaattcgt                                                                                             <  2:1955048/150‑1 (MQ=255)

                                                          tACCCAGGTGTACATCAGGCATTACCGCAATATGTTTGAAAATAAACGGCATCTTCGCCGTATTAATAAGTTGCTGACGCGCATCGGCCTCTACCGGCACGCCTTTGGTCCACATTTTTACCGGGGCATTTTCAGTGGTCAgtaattcgt                                                                                             >  2:1778046/1‑150 (MQ=255)

                                                             ccAGGTGTACATCAGGCATTACCGCAATATGTTTGAAAATAAACGGCATCTTCGCCGTATTAATAAGTTGCTGACGCGCATCGGCCTCTACCGGCACGCCTTTGGTCCACATTTTTACCGGGGCATTTTCAGGGTTCAgtaattcgtaat                                                                                          >  2:2923101/1‑150 (MQ=255)

                                                              cAGGTGTACATCAGGCATTACCGCAATATGTTTGAAAATAAACGGCATCTTCGCCGTATTAATAAGTTGCTGACGCGCATCGGCCTCTACCGGCACGCCTTTGGTCCACATTTTTACCGGGGCATTTTCAGTGGTCAgtaattcgtaatt                                                                                         <  1:2295975/150‑1 (MQ=255)

                                                                ggTGTACATCAGGCATTACCGCAATATGTTTGAAAATAAACGGCATCTTCGCCGTATTAATAAGTTGCTGACGCGCATCGGCCTCTACCGGCACGCCTTTGGTCCACATTTTTACCGGGGCATTTTCAGTGGTCAGTAATTCGTAATTCa                                                                                       <  1:4307106/150‑1 (MQ=255)

                                                                 gtgtACATCAGGCATTACCGCAATATGTTTGAAAATAAACGGCATCTTCGCCGTATTAATAAGTTGCTGACGCGCATCGGCCTCTACCGGCACGCCTTTGGTCCACATTTTTACCGGGGCATTTTCAGTGGTCAGTAATTCGTAATTCAt                                                                                      <  1:1115984/150‑1 (MQ=255)

                                                                  tgtACATCAGGCATTACCGCAATATGTTTGAAAATAAACGGCATCTTCGCCGTATTAATAAGTTGCTGACGCGCATCGGCCTCTACCGGCACGCCTTTGGTCCACATTTTTACCGGGGCATTTTCAGTGGTCAGTAATTCGTAATTCAtt                                                                                     <  1:1641087/150‑1 (MQ=255)

                                                                   gtACATCAGGCATTACCGCAATATGTTTGAAAATAAACGGCATCTTCGCCGTATTAATAAGTTGCTGACGCGCATCGGCCTCTACCGGCACGCCTTTGGTCCACATTTTTACCGGGGCATTCTCAGTGGTCAGTAATTCGTAATTCAttt                                                                                    <  2:369340/150‑1 (MQ=255)

                                                                    tACATCAGGCATTACCGCAATATTTTTGAAAATAAACGGCATCTTCGCCGTATTAATAAGTTGCTGACGCGCATCGGCCTCTACCGGCACGCCTTTGGTCCACATTTTTACCGGGGCATTTTCAGTGGTCAGTAATTCGTAATTCAtttt                                                                                   <  1:887689/150‑1 (MQ=255)

                                                                    tACATCAGGCATTACCGCAATATGTTTGAAAATAAACGGCATCTTCGCCGTATTAATAAGTTGCTGACGCGCATCGGCCTCTACCGGCACGCCTTTGGTCCACATTTTTACCGGGGCATTTTCAGTGGTCAGTAATTCGTAATTCAtttt                                                                                   <  2:2545598/150‑1 (MQ=255)

                                                                     aCATCAGGCATTACCGCAATATGTTTGAAAATAAACGGCATCTTCGCCGTATTAATAAGTTGCTGACGCGCATCGGCCTCTACCGGCACGCCTTTGGTCCACATTTTTACCGGGGCATTTTCAGTGGTCAGTAATTCGTAATTCATTTTg                                                                                  <  2:2292658/150‑1 (MQ=255)

                                                                      cATCAGGCATTACCGCAATATGTTTGAAAATAAACGGCATCTTCGCCGTATTAATAAGTTGCTGACGCGCATCGGCCTCTACCGGCACGCCTTTGGTCCACATTTTTACCGGGGCATTTTCAGTGGTCAGTAATTCGTAATTCATTTTGt                                                                                 >  2:3608951/1‑150 (MQ=255)

                                                                      cATCAGGCATTACCGCAATATGTTAGAAAATAAACGGCATCTTCGCCGTATTAATAAGTTGCTGACGCGCATCGGCCTCTACCGGCACGCCTTTGGTCCACATTTTTACCGGGGCATTTTCAGTGGTCAGTAATTCGTAATTCATTTTGt                                                                                 >  2:786109/1‑150 (MQ=255)

                                                                       aTCAGGCATTACCGCAATATGTTTGAAAATAAACGGCATCTTCGCCGTATTAATAAGTTGCTGACGCGCATCGGCCTCTACCGGCACGCCTTTGGTCCACATTTTTACCGGGGCATTTTCAGTGGTCAGTAATTCGTATTTCATtttttt                                                                                >  2:3509132/1‑150 (MQ=255)

                                                                       aTCAGGCATTACCGCAATATGTTTGAAAATAAACGGCATCTTCGCCGTATTAATAAGTTGCTGACGCGCATCGGCCTCTACCGGCACGCCTTTGGTCCACATTTTTACCGGGGCATTTTCAGTGGTCAGTAATTCGTAATTCATTTTGtt                                                                                >  2:3904138/1‑150 (MQ=255)

                                                                       aTCAGGCATTACCGCAATATGTTTGAAAATAAACGGCATCTTCGCCGTATTAATAAGTTGCTGACGCGCATCGGCCTCTACCGGCACGCCTTTGGTCCACATTTTTACCGGGGCATTTTCAGTGGTCAGTAATTCGTAATTCATTTTGtt                                                                                <  2:3412701/150‑1 (MQ=255)

                                                                        tCAGGCATTACCGCAATATGTTTGAAAATAAACGGCATCTTCGCCGTATTAATAAGTTGCTGACGCGCATCGGCCTCTACCGGCACGCCTTTGGTCCACATTTTTACCGGGGCATTTTCAGTGGTCAGTAATTCGTAATTCATTTTGttt                                                                               >  1:2038014/1‑150 (MQ=255)

                                                                        tCAGGCATTACCGCAATATGTTTGAAAATAAACGGCATCTTCGCCGTATTAATAAGTTGCTGACGCGCATCGGCCTCTACCGGCACGCCTTTGGTCCACATTTTTACCGGGGCATTTTCAGTGGTCAGTAATTCGTAATTCATTTTGttt                                                                               >  1:2158701/1‑150 (MQ=255)

                                                                        tCAGGCATTACCGCAATATGTTTGAAAATAAACGGCATCTTCGCCGTATTAATAAGTTGCTGACGCGCATCGGCCTCTACCGGCACGCCTTTGGTCCACATTTTTACCGGGGCATTTTCAGTGGTCAGTAATTCGTAATTCATTTTGttt                                                                               >  1:2159612/1‑150 (MQ=255)

                                                                         cAGGCATTACCGCAATATGTTTGAAAATAAACGGCATCTTCGCCGTATTAATAAGTTGCTGACGCGCATCGGCCTCGACCGGCACGCCTTTGGTCCACATTTTTACAGGGGCATTTTCAGTGGTAAGTAATTCGTAATTCATTTTGtttt                                                                              >  2:2785466/1‑150 (MQ=255)

                                                                          aGGCATTACCGCAATATGTTTGAAAATAAACGGCATCTTCGCCGTATTAATAAGTTGCTGACGCGCATCGGCCTCTACCGGCACGCCTTTGGTCCACATTTTTACCGGGGCATTTTCAGTGGTCAGTAATTCGTAATTCATTTTGttttt                                                                             <  1:3608906/150‑1 (MQ=255)

                                                                          aGGCATTACCGCAATATGTTTGAAAATAAACGGCATCTTCGCCGTATTAATAAGTTGCTGACGCGCATCGGCCTCTACCGGCACGCCTTTGGTCCACATTTTTACCGGGGCATTTTCAGTGGTCAGTAATTCGTAATTCATTTTGttttt                                                                             <  1:3378610/150‑1 (MQ=255)

                                                                           ggCATTACCGCAATATGTTTGAAAATAAACGGCATCTTCGCCGTATTAATAAGTTGCTGACGCGCATCGGCCTCGACCGGCACGCCTTTGGTCCACATTTTTACCGGGGCATTTTCAGTGGTCAGTAATTCGTAATTCATTTTGTTTTtc                                                                            <  1:4063097/150‑1 (MQ=255)

                                                                              aTTACCGCAATATGTTTGAAAATAAACGGCATCTTCGCCGTATTAATAAGTTGCTGACGCGCATCGGCCTCTACCGGCACGCCTTTGGTCCACATTTTTACCGGGGCATTTTCAGTGGTCAGTAATTCGTAATTCATTTTGTTTTTctct                                                                         <  1:1261813/150‑1 (MQ=255)

                                                                               ttACCGCAATATGTTTGAAAATAAACGGCATCTTCGCCGTATTAATAAGTTGCTGACGCGCATCGGCCTCTACCGGCACGCCTTTGGTCCACATTTTTACCGGGGCATTTTCAGTGGTCAGTAATTCGTAATTCATTTTGTTTTTCTCtt                                                                        <  1:3292009/150‑1 (MQ=255)

                                                                                  ccGCAATATGTTTGAAAATAAACGGCATCTTCGCCGTATTAATAAGTTGCTGACGCGCATCGGCCTCTACCGGCACGCCTTTGGTCCACATTTTTACCGGGGCATTTTCAGTGGTCAGTAATTCGTAATTCATTTTGTTTTTCTCTTTTc                                                                     >  2:445179/1‑150 (MQ=255)

                                                                                   cGCAATATGTTTGAAAATAAACGGCATCTTCGCCGTATTAATAAGTTGCTGACGCGCATCGGCCTCTACCGGCACGCCTTTGGTCCACATTTTTACCGGGGCATTTTCAGTGGTCAGTAATTCGTAATTCATTTTGTTTTTCTCTTTTCg                                                                    <  1:1910229/150‑1 (MQ=255)

                                                                                     cAATATGTTTGAAAATAAACGGCATCTTCGCCGTATTAATAAGTTGCTGACGCGCATCGGCCTCTACCGGCACGCCTTTGGTCCACATTTTTACCGGGGCATTTTCAGTGGTCAGTAATTCGTAATTCATTTTGTTTTTCTCTTTTCgtt                                                                  <  2:1370959/150‑1 (MQ=255)

                                                                                      aaTATGTTTGAAAATAAACGGCATCTTCGCCGTATTAATAAGTTGCTGACGCGCATCGGCCTCTACCGGCACGCCTTTGGTCCACATTTTTACCGGGGCATTTTCAGTGGTCAGTAATTCGTAATTCATTTTGTTTTTCTCTTTTCgttg                                                                 <  1:1929468/150‑1 (MQ=255)

                                                                                       atatGTTTGAAAATAAACGGCATCTTCGCCGTATTAATAAGTTGCTGACGCTCATCGGCCTCTACCGGCACGCCTTTTGTACACATTTTTACCGGGGCATTTTCAGTGGTCAGTAATTCGTTATTCATTTTGTTTTTCTCTTTTCgttgt                                                                <  1:2503017/150‑1 (MQ=255)

                                                                                        tatGTTTGAAAATAAACGGCATCTTCGCCGTATTAATAAGTTGCTGACGCGCATCGGCCTCTACCGGCACGCCTTTGGTCCACATTTTTACCGGGGCATTTTCAGTGGTCAGTAATTCGTAATTCATTTTGTTTTTCTCTTTTCgttgtt                                                               <  1:1704917/150‑1 (MQ=255)

                                                                                        tatGTTTGAAAATAAACGGCATCTTCGCCGTATTAATAAGTTGCTGACGCGCATCGGCCTCTACCGGCACGCCTTTGGTCCACATTTTTACCGGGGCATTTTCAGTGGTCAGTAATTCGTAATTCATTTTGTTTTTCTCTTTTCgttgtt                                                               <  1:4476617/150‑1 (MQ=255)

                                                                                                 aaaTAAACGGCATCTTCGCCGTATTAATAAGTTGCTGACGCGCATCGGCCTCTACCGGCACGCCTTTGGTCCACATTTTTACCGGGGCATTTTCAGTGGTCAGTAATTCGTAATTCATTTTGTTTTTCTCTTTTCGTTGTTTGCTGTCCt                                                      <  2:1026502/150‑1 (MQ=255)

                                                                                                 aaaTAAACGGCATCTTCGCCGTATTAATAAGTTGCTGACGCGCATCGGCCTCGACCGGCACGCCTTTGGTCCACATTTTTACCGGGGCATTTTCAGTGGTCAGTAATTCGTAATTCATTTTGTTTTTCTCTTTTCGTTGTTTGCTGTCCt                                                      <  1:1440039/150‑1 (MQ=255)

                                                                                                    tAAACGGCATCTTCGCCGTATTAATAAGTTGCTGACGCGCATCGGCCTCTACCGGCACGCCTTTGGTCCACATTTTTACCGGGGCATTTTCAGTGGTCAGTAATTCGTAATTCATTTTGTTTTTCTCTTTTCGTTGTTTGCTGTCCTGAt                                                   <  2:2202877/150‑1 (MQ=255)

                                                                                                       aCGGCATCTTCGCCGTATTAATAAGTTGCTGACGCGCATCGGCCTCTACCGGCACGCCTTTGGTCCACATTTTTACCGGGGCATTTTCAGTGGTCAGTAATTCGTAATTCATTTTGTTTTTCTCTTTTCGTTGTTTGCTGTCCTGATAAt                                                >  1:3694749/1‑150 (MQ=255)

                                                                                                         ggCATCTTCGCCGTATTAATAAGTTGCTGACGCGCATCGGCCTCTACCGGCACGCCTTTGGTCCACATTTTTACCGGGGCATTTTCAGTGGTCAGTAATTCGTAATTCATTTTGTTTTTCTCTTTTCGTTGTTTGCTGTCCTGATAATTg                                              >  1:276067/1‑150 (MQ=255)

                                                                                                            aTCTTCGCCGTATTAATAAGTTGCTGACGCGCATCGGCCTCTACCGGCACGCCTTTGGTCCACATTTTTACCGGGGCATTTTCAGTGGTCAGTAATTCGTAATTCATTTTGTTTTTCTCTTTTCGTTGTTTGCTGTCCTGATAATTGCaa                                           <  2:3998039/150‑1 (MQ=255)

                                                                                                              cTTCGCCGTATTAATAAGTTGCTGACGCGCATCGGCCTCTACCGGCACGCCTTTGGTCCACATTTTTACCGGGGCATTTTCAGTGGTCAGTAATTCGTAATTCATTTTGTTTTTCTCTTTTCGTTGTTTGCTGTCCTGATAATTGCAAcc                                         >  1:1036165/1‑150 (MQ=255)

                                                                                                                     gTATTAATAAGTTGCTGACGCGCATCGGCCTCTACCGGCACGCCTTTGGTCCACATTTTTACCGGGGCATTTTCAGTGGTCAGTAATTCGTAATTCATTTTGTTTTTCTCTTTTCGTTGTTTGCTGTCCTGATAATTGCAACCGTCGTGc                                  >  1:2233226/1‑150 (MQ=255)

                                                                                                                       attattATGTTGCTGTCGCTCATCGGCCTCTACCGGCACGCCTTTGGTGCACATTTTTACCGGGGCATTTTCAGTGGTCAGTAATTCGTAATTCATTTTGTTTTTCTCTTTTCGTTGTTTGCTGTCCTGATAATTGCAACCGTCGTTCCa                                <  2:2033685/150‑1 (MQ=255)

                                                                                                                       aTTAATAAGTTGCTGACGCGCATCGGCCTCTACCGGCACGCCTTTGGTCCACATTTTTACCGGGGCATTTTCAGTGGTCAGTAATTCGTAATTCATTTTGTTTTTCTCTTTTCGTTGTTTGCTGTCCTGATAATTGCAACCGTCGTGCCa                                >  1:2884877/1‑150 (MQ=255)

                                                                                                                           ataaGTTGCTGACGCGCATCGGCCTCTACCGGCACGCCTTTGGTCCACATTTTTACCGGGGCATTTTCAGTGGTCAGTAATTCGTAATTCATTTTGTTTTTCTCTTTTCGTTGTTTGCTGTCCTGATAATTGCAACCGTCGTGCCAGaaa                            >  1:4376199/1‑150 (MQ=255)

                                                                                                                             aaGTTGCTGACGCGCATCGGCCTCTACCGGCACGCCTTTGGTCCACATTTTTACCGGGGCATTTTCAGTGGTCAGTAATTCGTAATTCATTTTGTTTTTCTCTTTTCGTTGTTTGCTGTCCTGATAATTGCAACCGTCGTGCCAGAAAAt                          <  1:4030713/150‑1 (MQ=255)

                                                                                                                              aGTTGCTGACGCGCATCGGCCTCTACCGGCACGCCTTTGGTCCACATTTTTACCGGGGCATTTTCAGTGGTCAGTAATTCGTAATTCATTTTGTTTTTCTCTTTTCGTTGTTTGCTGTCCTGATAATTGCAACCGTCGTGCCAGAAAAtt                         >  2:3326485/1‑150 (MQ=255)

                                                                                                                                 tGCTGACGCGCATCGGCCTCTACCGGCACGCCTTTGGTCCACATTTTTACCGGGGCATTTTCAGTGGTCAGTAATTCGTAATTCATTTTGTTTTTCTCTTTTCGTTGTTTGCTGTCCTGATAATTGCAACCGTCGTGCCAGAAAATTaaa                      <  1:4201713/150‑1 (MQ=255)

                                                                                                                                  gCTGACGCTCCTCGGCCTCGACCGGCACGCCTTTGTTCAAGATTTTTACCGGGGCATTTTCAGTGGTCAGTAATTCGTAATTCATTTTGTTTTTCTCTTTTCGTTGTTTGCTGTCCTGATAATTGCAACCGTCGTGCCAGAAAATTAAAt                     <  2:404351/150‑1 (MQ=255)

                                                                                                                                  gCTGACGCGCATCGGCCTCTACCGGCACGCCTTTGGTCCACATTTTTACCGGGGCATTTTCAGTGGTCAGTAATTCGTAATTCATTTTGTTTTTCTCTTTTCGTTGTTTGCTGTCCTGATAATTGCAACCGTCGTGCCAGAAAATTAAAt                     <  2:135095/150‑1 (MQ=255)

                                                                                                                                   cTGACGCGCATCGGCCTCTACCGGCACGCCTTTGGTACACATTTTTACCGGGGCATTTTCAGTGGTCAGTAATACGTAATTCATTTTGATTTTCTCTTTTCGTTGTTTGCTGTCCTGATAATTGCAACCGTCGTGCCAGAAAATTAAATa                    <  2:4093546/150‑1 (MQ=255)

                                                                                                                                         cgcATCGGCCTCTACCGGCACGCCTTTGGTCCACATTTTTACCGGGGCATTTTCAGTGGTCAGTAATTCGTAATTCATTTTGTTTTTCTCTTTTCGTTGTTTGCTGTCCTGATAATTGCAACCGTCGTGCCAGAAAATTAAATAagcagc              <  1:1618364/150‑1 (MQ=255)

                                                                                                                                         cgcATCGGCCTCTACCGGCACGCCTTTGGTCCACATTTTTACCGGGGCATTTTCAGTGGTCAGTAATTCGTAATTCATTTTGTTTTTCTCTTTTCGTTGTTTGCTGTCCTGATAATTGCAACCGTCGTGCCAGAAAATTAAACAagcagc              <  2:3441097/150‑1 (MQ=255)

                                                                                                                                          gcATCGGCCTCTACCGGCACGCCTTTGGTCCACATTTTTACCGGGGCATTTTCAGTGGTCAGTAATTCGTAATTCATTTTGTTTTTCTCTTTTCGTTGTTTGCTGTCCTGATAATTGCAACCGTCGTGCCAGAAAATTAAATAAGCAGCt             <  1:1994303/150‑1 (MQ=255)

                                                                                                                                           cATCGGCCTCTACCGGCACGCCTTTGGTCCACATTTTTACCGGGGCATTTTCAGTGGTCAGTAATTCGTAATTCATTTTGTTTTTCTCTTTTCGTTGTTTGCTGTCCTGATAATTGCAACCGTCGTGCCAGAAAATTAAATAAGCAGCtt            <  1:2369328/150‑1 (MQ=255)

                                                                                                                                           cATCGGCCTCTACCGGCACGCCTTTGGTCCACATTTTTACCGGGGCATTTTCAGTGGTCAGTAATTCGTAATTCATTTTGTTTTTCTCTTTTCGTTGTTTGCTGTCCTGATAATTGCAACCGTCGTGCCAGAAAATTAAATAAGCAGCtt            <  2:2472679/150‑1 (MQ=255)

                                                                                                                                            aTCGGCCTCTACCGGCACGCCTTTGGTCCACATTTTTACCGGGGCATTTTCAGTGGTCAGTAATTCGTAATTCATTTTGTTTTTCTCTTTTCGTTGTTTGCTGTCCTGATAATTGCAACCGTCGTGCCAGAAAATTAAATAAGCAGCTTa           <  1:114808/150‑1 (MQ=255)

                                                                                                                                              cGGCCTCTACCGGCACGCCTTTGGTCCACATTTTTACCGGGGCATTTTCAGTGGTCAGTAATTCGTAATTCATTTTGTTTTTCTCTTTTCGTTGTTTGCTGTCCTGATAATTGCAACCGTCGTGCCAGAAAATTAAATAAGCAGCTTAAt         <  1:2835674/150‑1 (MQ=255)

                                                                                                                                               ggCCTCTACCGGCACGCCTTTGGTCCACATTTTTACCGGGGCATTTTCAGTGGTCAGTAATTCGTAATTCATTTTGTTTTTCTCTTTTCGTTGTTTGCTGTCCTGATAATTGCAACCGTCGTGCCAGAAAATTAAATAAGCAGCTTAAtt        >  2:186862/1‑150 (MQ=255)

                                                                                                                                               ggCCTCTACCGGCACGCCTTTGGTCCACATTTTTACCGGGGCATTTTCAGTGGTCAGTAATTCGTAATTCATTTTGTTTTTCTCTTGTCGTTGTTTGCTGTCCTGATAATTGCAACCGTCGTGCCAGAAAATTAAATAAGCAGCTTAAtt        <  2:686536/150‑1 (MQ=255)

                                                                                                                                                gCCTCTACCGGCACGCCTTTGGTCCACAATTTTACCGGGGCATTTTCAGTGGTCAGTAATTCGTAATTCATTTTGTTTTTCTCTTTTCGTTGTTTGCTGTCCTGATAATTGCAACCGTCGTGCCAGAAAATTAAACAAGCAGCTTAAttt       <  1:1038936/150‑1 (MQ=255)

ACCGCCGCCGGAATAATCGCCCCTTTGGTCGGGATCACGCTACCAATGGTGGAACCTTTACCCAGGTGTACATCAGGCATTACCGCAATATGTTTGAAAATAAACGGCATCTTCGCCGTATTAATAAGTTGCTGACGCGCATCGGCCTCTACCGGCACGCCTTTGGTCCACATTTTTACCGGGGCATTTTCAGTGGTCAGTAATTCGTAATTCATTTTGTTTTTCTCTTTTCGTTGTTTGCTGTCCTGATAATTGCAACCGTCGTGCCAGAAAATTAAATAAGCAGCTTAATTTTTTAA  >  CP009273/3551224‑3551522

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`