Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,625,721 A→T 21.6% I77N (ATT→AAT)  yhiJ ← DUF4049 family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,625,7210AT21.6% 500.2 / 137.5 213I77N (ATT→AAT) yhiJDUF4049 family protein
Reads supporting (aligned to +/- strand):  ref base A (93/74);  new base T (20/26);  total (113/100)
Fisher's exact test for biased strand distribution p-value = 1.82e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.59e-01

Only 100 of 215 total aligned reads displayed.

AATATCGGTATTATCAAGAGAGCAACCGTAATTTTTGCTATTAATTGTCACAATAAACTGATAATTATTATTCCTACGTAGAATATGAGCACGCATGCCCGATAGCATACTATATTTTTCTGAATGATCCGTTTGTATGGTAAATGAAATTTTGTCATCTTGAATAGATTGTTGGAACCGTTCTATTTGCCAATCATCTATTTTTTCCACAACCTCACCGAGCATTGTCCTGGCAATACTTGATATATCATCACCTATACCATTTGCAATATACCCAAACAGTTCTTTAGTGAAGTT  >  CP009273/3625573‑3625869
                                                                                                                                                    |                                                                                                                                                    
aaTATCGGTATTATCAAGAGAGCAACCGTAATTTTTGCTATTAATTGTCACAATAAACTGATAATTATTATTCCTACGTAGAATATGAGCACGCATGCCCGATAGCATACTATATTTTTCTGAATGATCCGTTTGTATGGTAAATGAAAt                                                                                                                                                     >  1:247277/1‑150 (MQ=255)
 atatCGGTATTATCGAGAGAGCAACCGTAATTTTTGCTATTAATTGTCACAATAAACTGATAATTATTATTCCTACGTAGAATATGAGCACGCATGCCCGATAGCATACTATATTTTTCTGAATGATCCGTTTGTATGGTAAATGAAAtt                                                                                                                                                    <  1:1185775/150‑1 (MQ=255)
  tatCGGTATTATCAAGAGAGCAACCGTAATTTTTGCTATTAATTGTCACAATAAACTGATAATTATTATTCCTACGTAGAATATGAGCACGCATGCCCGATAGCATACTATATTTTTCTGAATGATCCGTTTGTATGGTAAATGAAAttt                                                                                                                                                   >  1:1758853/1‑150 (MQ=255)
       gTATTATCAAGAGAGCAACCGTAATTTTTGCTATTAATTGTCACAATAAACTGATAATTATTATTCCTACGTAGAATATGAGCACGCATGCCCGATAGCATACTATATTTTTCTGAATGATCCGTTTGTATGGTAAATGAAATTTTGTCa                                                                                                                                              <  2:1601469/150‑1 (MQ=255)
       gTATTATCAAGAGAGCAACCGTAATTTTTGCTATTAATTGTCACAATAAACTGATAATTATTATTCCTACGTAGAATATGAGCACGCATGCCCGATAGCATACTATATTTTTCTGAATGATCCGTTTGTATGGTAAATGAAATTTTGTCa                                                                                                                                              >  1:2193146/1‑150 (MQ=255)
         attatCAAGAGAGCAACCGTAATTTTTGCTATTAATTGTCACAATAAACTGATAATTATTATTCCTACGTAGAATATGAGCACGCATGCCCGATAGCATACTATATTTTTCTGAATGATCCGTTTGTATGGTAAATGAAATTTTGTCATc                                                                                                                                            >  1:3777910/1‑150 (MQ=255)
          ttatCAAGAGAGCAACCGTAATTTTTGCTATTAATTGTCACAATAAACTGATAATTATTATTCCTACGTAGAATATGAGCACGCATGCCCGATAGCATACTATATTTTTCTGAATGATCCGTTTGTATGGTAAATGAAATTTTGTCATCt                                                                                                                                           >  2:183674/1‑150 (MQ=255)
                 gagagCAACCGTAATTTTTGCTATTAATTGTCACAATAAACTGATAATTATTATTCCTACGTAGAATATGAGCACGCATGCCCGATAGCATACTATATTTTTCTGAATGATCCGTTTGTATGGTAAATGAATTTTTGTCATCTTGAATAg                                                                                                                                    <  1:986386/150‑1 (MQ=255)
                  agagCAACCGTAATTTTTGCTATTAATTGTCACAATAAACTGATAATTATTATTCCTACGTAGAATATGAGCACGCATGCCCGATAGCATACTATATTTTTCTGAATGATCCGTTTGTATGGTAAATGAAATTTTGTCATCTTGAATAGa                                                                                                                                   >  1:1955578/1‑150 (MQ=255)
                    agCAACCGTAATTTTTGCTATTAATTGTCACAATAAACTGATAATTATTATTCCTACGTAGAATATGAGCACGCATGCCCGATAGCATACTATATTTTTCTGAATGATCCGTTTGTATGGTAAATGAAATTTTGTCATCTTGAATAGAtt                                                                                                                                 >  2:4379917/1‑150 (MQ=255)
                    agCAACCGTAATTTTTGCTATTAATTGTCACAATAAACTGATAATTATTATTCCTACGTAGAATATGAGCACGCATGCCCGATAGCATACTATATTTTTCTGAATGATCAGTTTGTATGGTAAATGAAATTTTGTCATCTTGAATAGAtt                                                                                                                                 >  2:2375327/1‑150 (MQ=255)
                     gCAACCGTAATTTTTGCTATTAATTGTCACAATAAACTGATAATTATTATTCCTACGTAGAATATGAGCACGCATGCCCGATAGCATACTATATTTTTCTGAATGATCCGTTTGTATGGTAAATGAATTTTTGTCATCTTGAATAGAttg                                                                                                                                <  2:334444/150‑1 (MQ=255)
                       aaCCGTAATTTTTGCTATTAATTGTCACAATAAACTGATAATTATTATTCCTACGTAGAATATGAGCACGCATGCCCGATAGCATACTATATTTTTCTGAATGATCCGTTTGTATGGTAAATGAAATTTTGTCATCTTGAATAGAttgtt                                                                                                                              >  2:2736052/1‑150 (MQ=255)
                       aaCCGTAATTTTTGCTATTAATTGTCACAATAAACTGATAATTATTATTCCTACGTAGAATATGAGCACGCATGCCCGATAGCATACTATATTTTTCTGAATGATCCGTTTGTATGGTAAATGAAATTTTGTCATCTTGAATAGAttgtt                                                                                                                              >  1:2407295/1‑150 (MQ=255)
                        aCCGTAATTTTTGCTATTAATTGTCACAATAAACTGATAATTATTATTCCTACGTAGAATATGAGCACGCATGCCCGATAGCATACTATATTTTTCTGAATGATCCGTTTGTATGGTAAATGAATTTTTGTCATCTTGAATAGAttgttg                                                                                                                             <  1:778923/150‑1 (MQ=255)
                        aCCGTAATTTTTGCTATTAATTGTCACAATAAACTGATAATTATTATTCCTACGTAGAATATGAGCACGCATGCCCGATAGCATACTATATTTTTCTGAATGATCCGTTTGTATGGTAAATGAAATTTTGTCATCTTGAATAGAttgttg                                                                                                                             <  1:53060/150‑1 (MQ=255)
                        aCCGTAATTTTTGCTATTAATTGTCACAATAAACTGATAATTATTATTCCTACGTAGAATATGAGCACGCATGCCCGATAGCATACTATATTTTTCTGAATGATCCGTTTGTATGGTAAATGAAATTTTGTCATCTTGAATAGAttgttg                                                                                                                             <  2:3720429/150‑1 (MQ=255)
                         ccGTAATTTTTGCTATTAATTGTCACAATAAACTGATAATTATTATTCCTACGTAGAATATGAGCACGCATGCCCGATAGCATACTATATTTTTCTGAATGATCCGTTTGTATGGTAAATGAAATTTTGTCATCTTGAATAGATTGTTgg                                                                                                                            <  2:3014864/150‑1 (MQ=255)
                          agTAATTTTTGCTATTAATTGTCACAATAAACTGATAATTATTATTCCTACGTAGAATATGAGCACGCATGCCCGATAGCATACTATATTTTTCTGAATGATCCGTTTGTATGGTAAATGAAATTTTGTCATCTTGAATAGATTGTTGGa                                                                                                                           <  2:2365852/149‑1 (MQ=255)
                           gTAATTTTTGCTATTAATTGTCACAATAAACTGATAATTATTATTCCTACGTAGAATATGAGCACGCATGCCCGATAGCATACTATATTTTTCTGAATGATCCGTTTGTATGGTAAATGAAATTTTGTCATCTTGAATAGATTGTTGGaa                                                                                                                          >  2:391763/1‑150 (MQ=255)
                             aaTTTTTGCTATTAATTGTCACAATAAACTGATAATTATTATTCCTACGTAGAATATGAGCACGCATGCCCGATAGCATACTATATTTTTCTGAATGATCCGTTTGTATGGTAAATGAAATTTTGTCATCTTGAATAGATTGTTGGAAcc                                                                                                                        >  1:3960995/1‑150 (MQ=255)
                               tttttGCTATTAATTGTCACAATAAACTGATAATTATTATTCCTACGTAGAATATGAGCACGCATGCCCGATAGCATACTATATTTTTCTGAATGATCCGTTTGTATGGTAAATGAAATTTTGTCATCTTGAATAGATTGTTGGAACCGt                                                                                                                      <  1:4424929/150‑1 (MQ=255)
                                 tttGCTATTAATTGTCACAATAAACTGATAATTATTATTCCTACGTAGAATATGAGCACGCATGCCCGATAGCATACTATATTTTTCTGAATGATCCGTTTGTATGGTAAATGAATTTTTGTCATCTTGAATAGATTGTTGGAACCGTTc                                                                                                                    >  2:4249596/1‑150 (MQ=255)
                                 tttGCTATTAATTGTCACAATAAACTGATAATTATTATTCCTACGTAGAATATGAGCACGCATGCCCGATAGCATACTATATTTTTCTGAATGATCCGTTTGTATGGTAAATGAAATTTTGTCATCTTGAATAGATTGTTGGAACCGTTc                                                                                                                    >  1:4131714/1‑150 (MQ=255)
                                 tttGCTATTAATTGTCACAATAAACTGATAATTACTATTCCTACGTAGAATATGAGCACGCATGCCCGATAGCATACTATATTTTTCTGAATGATCCGTTTGTATGGTAAATGAATTTTTGTCATCTTGAATAGATTGTTGGAACCGTTc                                                                                                                    >  1:2120515/1‑150 (MQ=255)
                                 tttGCTAGTCATTGTCACAATAAACTGAGAATTATTATTCCTACGTAGAATATGAGCACGCATGCCCGATAGCATACTATATTTTTCTGAATGATCCGTTTGTATGGTAAATGAAATTTTGTCATCTTGAATAGATTGTTGGAACCGTTc                                                                                                                    <  2:3501687/150‑1 (MQ=255)
                                   tGCTATTAATTGTCACAATAAACTGATAATTATTATTCCTACGTAGAATATGAGCACGCATGCCCGATAGCATACTATATTTTTCTGAATGATCCGTTTGTATGGTAAATGAATTTTTGTCATCTTGAATAGATTGTTGGAACCGTTCTa                                                                                                                  <  2:124007/150‑1 (MQ=255)
                                   tGCTATTAATTGTCACAATAAACTGATAATTATTATTCCTACGTAGAATATGAGCACGCATGCCCGATAGCATACTATATTTTTCTGAATGATCCGTTTGTATGGTAAATGAAATTTTGTCATCTTGAATAGATTGTTGGAACCGTTCTa                                                                                                                  >  1:3116732/1‑150 (MQ=255)
                                      tATTAATTGTCACAATAAACTGATAATTATTATTCCTACGTAGAATATGAGCACGCATGCCCGATAGCATACTATATTTTTCTGAATGATCCGTTTGTATGGTAAATGAAATTTTGTCATCTTGAATAGATTGTTGGAACCGTTCTAttt                                                                                                               <  2:1684341/150‑1 (MQ=255)
                                      tATTAATTGTCACAATAAACTGATAATTATTATTCCTACGTAGAATATGAGCACGCATGCCCGATAGCATACTATATTTTTCTGAATGATCCGTTTGTATGGTAAATGAAATTTTGTCATCTTGAATAGATTGTTGGAACCGTTCTAttt                                                                                                               >  2:3776891/1‑150 (MQ=255)
                                           aTTGTCACAATAAACTGATAATTATTATTCCTACGTAGAATATGAGCACGCATGCCCGATAGCATACTATATTTTTCTGAATGATCCGTTTGTATGGTAAATGAATTTTTGTCATCTTGAATAGATTGTTGGAACCGTTCTATTTGCCaa                                                                                                          >  1:201160/1‑150 (MQ=255)
                                              gTCACAATAAACTGATAATTATTATTCCTACGTAGAATATGAGCACGCATGCCCGATAGCATACTATATTTTTCTGAATGATCCGTTTGTATGGTAAATGAAATTTTGTCATCTTGAATAGATTGTTGGAACCGTTCTATTTGCCAatca                                                                                                       <  1:1608094/150‑1 (MQ=255)
                                                cacaATAAACTGATAATTATTATTCCTACGTAGAATATGAGCACGCATGCCCGATAGCATACTATATTTTTCTGAATGATCCGTTTGTATGGTAAATGAAATTTTGTCATCTTGAATAGATTGTTGGAACCGTTCTATTTGCCAatcatc                                                                                                     >  1:4317367/1‑150 (MQ=255)
                                                  caATAAACTGATAATTATTATTCCTACGTAGAATATGAGCACGCATGCCCGATAGAATACTATATTTTTCTGAATGATCCGTTTGTATGGTAAATGAAATTTTGTCATCTTGAATAGATTGTTGGAACCGTTCTATTTGCCAATCATCTa                                                                                                   >  2:2426150/1‑150 (MQ=255)
                                                   aaTAAACTGATAATTATTATTCCTACGTAGAATATGAGCACGCATGCCCGATAGCATACTATATTTTTCTGAATGATCCGTTTGTATGGTAAATGAAATTTTGTCATCTTGAATAGATTGTTGGAACCGTTCTATTTGCCAATCATCTAt                                                                                                  >  2:4093752/1‑150 (MQ=255)
                                                   aaTAAACTGATAATTATTATTCCTACGTAGAATATGAGCACGCATGCCCGATAGCATACTATATTTTTCTGAATGATCCGTTTGTATGGTAAATGAAATTTTGTCATCTTGAATAGATTGTTGGAACCGTTCTATTTGCCAATCATCTAt                                                                                                  >  2:70366/1‑150 (MQ=255)
                                                    aTAAACTGATAATTATTATTCCTACGTAGAATATGAGCACGCATGCCCGATAGCATACTATATTTTTCTGAATGATCCGTTTGTATGGTAAATGAAATTTTGTCATCTTGAATAGATTGTTGGAACCGTTCTATTTGCCAATCATCTAtt                                                                                                 <  2:4277650/150‑1 (MQ=255)
                                                     tAAACTGATAATTATTATTCCTACGTAGAATATGAGCACGCATGCCCGATAGCATACTATATTTTTCTGAATGATCCGTTTGTATGGTAAATGAAATTTTGTCATCTTGAATAGATTGTTGGAACCGTTCTATTTGCCAATCATCTAttt                                                                                                <  2:3536396/150‑1 (MQ=255)
                                                      aaaCTGATAATTATTATTCCTACGTAGAATATGAGCACGCATGCCCGATAGCATACTATATTTTTCTGAATGATCCGTTTGTATTGTAAATGAAATTTTGTCATCTTGAATAGATTGTTGGAACCGTTCTATTTGCCAATCATCTAtttt                                                                                               <  1:1342381/150‑1 (MQ=255)
                                                       aaCTGATAATTATTATTCCTACGTAGAATATGAGCACGCATGCCCGATAGCATACTATATTTTTCTGAATGATCCGTTTGTATGGTAAATGAAATTTTGTCATCTTGAATAGATTGTTGGAACCGTTCTATTTGCCAATCATCTAttttt                                                                                              <  2:1163291/150‑1 (MQ=255)
                                                        aCTGATAATTATTATTCCTACGTAGAATATGAGCACGCATGCCCGATAGCATACTATATTTTTCTGAATGATCCGTTTGTATGGTAAATGAAATTTTGTCATCTTGAATAGATTGTTGGAACCGTTCTATTTGCCAATCATCTAtttttt                                                                                             >  2:2285314/1‑150 (MQ=255)
                                                            aTAATTATTATTCCTACGTAGAATATGAGCACGCATGCCCGATAGCATACTATATTTTTCTGAATGATCCGTTTGTATGGTAAATGAAATTTTGTCATCTTGAATAGATTGTTGGAACCGTTCTATTTGCCAATCATCTATTTTTTCcac                                                                                         <  1:658185/150‑1 (MQ=255)
                                                            aTAATTATTATTCCTACGTAGAATATGAGCACGCATGCCCGATAGCATACTATATTTTTCTGAATGATCCGTTTGTATGGTAAATGAAATTTTGTCATCTTGAATAGATTGTTGGAACCGTTCTATTTGCCAATCATCTATTTTTTCcac                                                                                         <  2:1058556/150‑1 (MQ=255)
                                                             tAATTATTATTCCTACGTAGAATATGAGCACGCATGCCCGATAGCATACTATATTTTTCTGAATGATCCGTTTGTATGGTAAATGAAATTTTGTCATCTTGAATAGATTGTTGGAACCGTTCTATTTGCCAATCATCTATTTTTTCcaca                                                                                        >  1:242567/1‑150 (MQ=255)
                                                                 tattattCCTACGTAGAATATGAGCACGCATGCCCGATAGCATACTATATTTTTCTGAATGATCCGTTTGTATGGTAAATGAAATTTTGTCATCTTGAATAGATTGTTGGAACCGTTCTATTTGCCAATCATCTATTTTTTCCACAACCt                                                                                    >  2:4043671/1‑150 (MQ=255)
                                                                    tattCCTACGTAGAATATGAGCACGCATGCCCGATAGCATACTATATTTTTCTGAATGATCCGTTTGTATGGTAAATGAAATTTTGTCATCTTGAATAGATTGTTGGAACCGTTCTATTTGCCAATCATCTATTTTTTCCACAACCTCAc                                                                                 <  2:3461459/150‑1 (MQ=255)
                                                                      ttCCTACGTAGAATATGAGCACGCATGCCCGATAGCATACTATATTTTTCTGAATGATCCGTTTGTATGGTAAATGAAATTTTGTCATCTTGAATAGATTGTTGGAACCGTTCTATTTGCCAATCATCTATTTTTTCCACAACCTCACCg                                                                               <  2:1439617/150‑1 (MQ=255)
                                                                      ttCCTACGTAGAATATGAGCACGCATGCCCGATAGCATACTATATTTTTCTGAATGATCCGTTTGTATGGTAAATGAAATTTTGTCATCTTGAATAGATTGTTGGAACCGTTCTATTTGCCAATCATCTATTTTTTCCACAACCTCACCg                                                                               <  2:2994950/150‑1 (MQ=255)
                                                                       tCCTACGTAGAATATGAGCACGCATGCCCGATAGCATACTATATTTTTCTGAATGATCCGTTTGTATGGTAAATGAAATTTTGTCATCTTGAATAGATTGTTGGAACCGTTCTATTTGCCAATCATCTATTTTTTCCACAACCTCACCGa                                                                              >  2:2567408/1‑150 (MQ=255)
                                                                        ccTACGTAGAATATGAGCACGCATGCCCGATAGCATACTATATTTTTCTGAATGATCCGTTTGTATGGTAAATGAAATTTTGTCATCTTGAATAGATTGTTGGAACCGTTCTATTTGCCAATCATCTATTTTTTCCACAACCTCACCGAg                                                                             >  1:3167678/1‑150 (MQ=255)
                                                                           aCGTAGAATATGAGCACGCATGCCCGATAGCATACTATATTTTTCTGAATGATCCGTTTGTATGGTAAATGAAATTTTGTCATCTTGAATAGATTGTTGGAACCGTTCTATTTGCCAATCATCTATTTTTTCCACAACCTCACCGAGCAt                                                                          <  2:3940206/150‑1 (MQ=255)
                                                                            cGTAGAATATGAGCACGCATGCCCGATAGCATACTATATTTTTCTGAATGATCCGTTTGTATGGTAAATGAAATTTTGTCATCTTGAATAGATTGTTGGAACCGTTCTATTTGCCAATCATCTATTTTTTCCACAACCTCACCGAGCAtt                                                                         <  2:4131714/150‑1 (MQ=255)
                                                                               aGAATATGAGCACGCATGCCCGATAGCATACTATATTTTTCTGAATGATCCGTTTGTATGGTAAATGAAATTTTGTCATCTTGAATAGATTGTTGGAACCGTTCTATTTGCCAATCATCTATTTTTTCCACAACCTCACCGAGCATTGTc                                                                      <  1:2887723/150‑1 (MQ=255)
                                                                                 aaTATGAGCACGCATGCCCGATAGCATACTATATTTTTCTGAATGATCCGTTTGTATGGTAAATGAAATTTTGTCATCTTGAATAGATTGTTGGAACCGTTCTATTTGCCAATCATCTATTTTTTCCACAACCTCACCGAGCATTGTCCt                                                                    <  2:3626432/150‑1 (MQ=255)
                                                                                  atatGAGCACGCATGCCCGATAGCATACTATATTTTTCTGAATGATCCGTTTGTATGGTAAATGAAATTTTGTCATCTTGAATAGATTGTTGGAACCGTTCTATTTGCCAATCATCTATTTTTTCCACAACCTCACCGAGCATTGTCCTg                                                                   >  1:1077842/1‑150 (MQ=255)
                                                                                    atGAGCACGCATGCCCGATAGCATACTATATTTTTCTGAATGATCCGTTTGTATGGTAAATGAAATTTTGTCATCTTGAATAGATTGTTGGAACCGTTCTATTTGCCAATCATCTATTTTTTCCACAACCTCACCGAGCATTGTCCTGGc                                                                 >  1:2296730/1‑150 (MQ=255)
                                                                                    atGAGCACGCATGCCCGATAGCATACTATATTTATCTGAATGATCCGTTTGTATGGAAAATTAAATTTTGTAATATTGAATAGATTGTTGGAACCGTTCTATTTGACAATCATCTATTTTTTCCACAACCTCACCTAGCATTGTCCTGGc                                                                 >  1:2296465/1‑150 (MQ=255)
                                                                                       aGCACGCATGCCCGATAGCATACTATATTTTTCTGAATGATCCGTTTGTATGGTAAATGAATTTTTGTCATCTTGAATAGATTGTTGGAACCGTTCTATTTGCCAATCATCTATTTTTTCCACAACCTCACCGAGCATTGTCCTGGCAAt                                                              >  1:3189365/1‑150 (MQ=255)
                                                                                        gCACGCATGCCCGATAGCATACTATATTTTTCTGAATGATCCGTTTGTATGGTAAATGAAATTTTGTCATCTTGAATAGATTGTTGGAACCGTTCTATTTGCCAATCATCTATTTTTTCCACAACCTCACCGAGCATTGTCCTGGCAATa                                                             <  1:2628803/150‑1 (MQ=255)
                                                                                         cACGCATGCCCGATAGCATACTATATTTTTCTGAATGATCCGTTTGTATGGTAAATGAAATTTTGTCATCTTGAATAGATTGTTGGAACCGTTCTATTTGCCAATCATCTATTTTTTCCACAACCTCACCGAGCATTGTCCTGGCAATAc                                                            <  2:792251/150‑1 (MQ=255)
                                                                                            gCATGCCCGATAGCATACTATATTTTTCTGAATGATCCGTTTGTATGGTAAATGAAATTTTGTCATCTTGAATAGATTGTTGGAACCGTTCTATTTGCCAATCATCTATTTTTTCCACAACCTCACCGAGCATTGTCCTGGCAATACTTg                                                         <  1:473635/150‑1 (MQ=255)
                                                                                             cATGCCCGATAGCATACTATATTTTTCTGAATGATCCGTTTGTATGGTAAATGAAATTTTGTCATCTTGAATAGATTGTTGGAACCGTTCTATTTGCCAATCATCTATTTTTTCCACAACCTCACCGAGCATTGTCCTGGCAATACTTGa                                                        >  1:3856069/1‑150 (MQ=255)
                                                                                                 cccGATAGCATACTATATTTTTCTGAATGATCCGTTTGTATGGTAAATGAATTTTTGTCATCTTGAATAGATTGTTGGAACCGTTCTATTTGCCAATCATCTATTTTTTCCACAACCTCACCGAGCATTGTCCTGGCAATACTTGatata                                                    <  1:3459224/150‑1 (MQ=255)
                                                                                                 cccGATAGCATACTATATTTTTCTGAATGATCCGTTTGTATGGTAAATGAATTTTTGTCATCTTGAATAGATTGTTGGAACCGTTCTATTTGCCAATCATCTATTTTTTCCACAACCTCACCGAGCATTGTCCTGGCAATACTTGatata                                                    <  1:978399/150‑1 (MQ=255)
                                                                                                  ccGATAGCATACTATATTTTTCTGAATGATCCGTTTGTATGGTAAATGAAATTTTGTCATCTTGAATAGATTGTTGGAACCGTTCTATTTGCCAATCATCTATTTTTTCCACAACCTCACCGAGCATTGTCCTGGCAATACTTGatatat                                                   >  2:1312423/1‑150 (MQ=255)
                                                                                                     aTAGCATACTATATTTTTCTGAATGATCCGTTTGTATGGTAAATGAATTTTTGTCATCTTGAATAGATTGTTGGAACCGTTCTATTTGCCAATCATCTATTTTTTCCACAACCTCACCGAGCATTGTCCTGGCAATACTTGATATatcat                                                >  2:4492763/1‑150 (MQ=255)
                                                                                                     aTAGCATACTATATTTTTCTGAATGATCCGTTTGTATGGTAAATGAATTTTTGTCATCTTGAATAGATTGTTGGAACCGTTCTATTTGCCAATCATCTATTTTTTCCACAACCTCACCGAGCATTGTCCTGGCAATACTTGATATatcat                                                <  2:553714/150‑1 (MQ=255)
                                                                                                      tAGCATACTATATTTTTCTGAATGATCCGTTTGTATGGTAAATGAAATTTTGTCATCTTGAATAGATTGTTGGAACCGTTCTATTTGCCAATCATCTATTTTTTCCACAACCTCACCGAGCATTGTCCTGGCAATACTTGATATatcatc                                               >  2:4238615/1‑150 (MQ=255)
                                                                                                        gCATACTATATTTTTCTGAATGATCCGTTTGTATGGTAAATGAAATTTTGTCATCTTGAATAGATTGTTGGAACCGTTCTATTTGCCAATCATCTATTTTTTCCACAACCTCACCGAGCATTGTCCTGGCAATACTTTATATATCATCAc                                             >  1:1509496/1‑150 (MQ=255)
                                                                                                        gCATACTATATTTTTCTGAATGATCCGTTTGTATGGTAAATGAAATTTTGTCATCTTGAATAGATTGTTGGAACCGTTCTATTTGCCAATCATCTATTTTTTCCACAACCTCACCGAGCATTGTCCTGGCAATACTTGATATATCATCAc                                             >  2:674429/1‑150 (MQ=255)
                                                                                                         cATACTATATTTTTCTGAATGATCCGTTTGTATGGTAAATGAATTTTTGTCATCTTGAATAGATTGTTGGAACCGTTCTATTTGCCAATCATCTATTTTTTCCACAACCTCACCGAGCATTGTCCTGGCAATACTTGATATATCATCAcc                                            <  1:4247011/150‑1 (MQ=255)
                                                                                                            aCTATATTTTTCTGAATGATCCGTTTGTATGGTAAATGAAATTTTGTCATCTTGAATAGATTGTTGGAACCGTTCTATTTGCCAATCATCTATTTTTTCCACAACCTCACCGAGCATTGTCCTGGCAATACTTGATATATCATCACCtat                                         >  2:3013032/1‑150 (MQ=255)
                                                                                                              tataTTTTTCTGAATGATCCGTTTGTATGGTAAATGAATTTTTGTCATCTTGAATAGATTGTTGGAACCGTTCTATTTGCCAATCATCTATTTTTTCCACAACCTCACCGAGCATTGTCCTGGCAATACTTGATATATCATCACCTATAc                                       <  2:3102521/150‑1 (MQ=255)
                                                                                                               atatTTTTCTGAATGATCCGTTTGTATGGTAAATGAAATTTTGTCATCTTGAATAGATTGTTGGAACCGTTCTATTTGCCAATCATCTATTTTTTCCACAACCTCACCGAGCATTGTCCTGGCAATACTTGATATATCATCACCTATAcc                                      >  1:2049407/1‑150 (MQ=255)
                                                                                                               atatTTTTCTGAATGATCCGTTTGTATGGTAAATGAAATTTTGTCATCTTGAATAGATTGTTGGAACCGTTCTATTTGCCAATCATCTATTTTTTCCACAACCTCACCGAGCATTGTCCTGGCAATACTTGATATATCATCACCTATAcc                                      <  1:2003455/150‑1 (MQ=255)
                                                                                                                 atTTTTCTGAATGATCCGTTTGTATGGTAAATGAATTTTTGTCATCTTGAATAGATTGTTGGAACCGTTCTATTTGCCAATCATCTATTTTTTCCACAACCTCACCGAGCATTGTCCTGGCAATACTTGATATATCATCACCTATACCAt                                    >  2:2580139/1‑150 (MQ=255)
                                                                                                                    tttCTGAATGATCCGTTTGTATGGTAAATGAATTTTTGTCATCTTGAATAGATTGTTGGAACCGTTCTATTTGCCAATCATCTATTTTTTCCACAACCTCACCGAGCATTGTCCTGGCAATACTTGATATATCATCACCTATACCATTTg                                 <  1:291616/150‑1 (MQ=255)
                                                                                                                     ttCTGAATGATCCGTTTGTATGGTAAATGAATTTTTGTCATCTTGAATAGATTGTTGGAACCGTTCTATTTGCCAATCATCTATTTTTTCCACAACCTCACCGAGCATTGTCCTGGCAATACTTGATATATCATCACCTATACCATTTGc                                <  2:2193055/150‑1 (MQ=255)
                                                                                                                       cTGAATGATCCGTTTGTATGGTAAATGAATTTTTGTCATCTTGAATAGATTGTTGGAACCGTTCTATTTGCCAATCATCTATTTTTTCCACAACCTCACCGAGCATTGTCCTGGCAATACTTGATATATCATCACCTATACCATTTGCaa                              >  2:1737935/1‑150 (MQ=255)
                                                                                                                        tGAATGATCCGTTTGTATGGTAAATGAAATTTTGTCATCTTGAATAGATTGTTGGAACCGTTCTATTTGCCAATCATCTATTTTTTCCACAACCTCACCGAGCATTGTCCTGGCAATACTTGATATATCATCACCTATACCATTTGCAat                             <  2:269272/150‑1 (MQ=255)
                                                                                                                         gAATGATCCGTTTGTATGGTAAATGAAATTTTGTCATCTTGAATAGATTGTTGGAACCGTTCTATTTGCCAATCATCTATTTTTTCCACAACCTCACCGAGCATTGTCCTGGCAATACTTGATATATCATCACCTATACCATTTGCAata                            <  2:4431066/150‑1 (MQ=255)
                                                                                                                          aaTGATCCGTTTGTATGGTAAATGAAATTTTGTCATCTTGAATAGATTGTTGGAACCGTTCTATTTGCCAATCATCTATTTTTTCCACAACCTCACCGAGCATTGTCCTGGCAATACTTGATATATCATCACCTATACCATTTGCAatat                           <  1:392121/150‑1 (MQ=255)
                                                                                                                           aTGATCCGTTTGTATGGTAAATGAAATTTTGTCATCTTGAATAGATTGTTGGAACCGTTCTATTTGCCAATCATCTATTTTTTCCACAACCTCACCGAGCATTGTCCTGGCAATACTTGATATATCATCACCTATACCATTTGCAAtata                          >  2:2714311/1‑150 (MQ=255)
                                                                                                                             gATCCGTTTGTATGGTAAATGAAATTTTGTCATCTTGAATAGATTGTTGGAACCGTTCTATTTGCCAATCATCTATTTTTTCCACAACCTCACCGAGCATTGTCCTGGCAATACTTGATATATCATCACCTATACCATTTGCAATATAcc                        >  2:1965741/1‑150 (MQ=255)
                                                                                                                              aTCCGTTTGTATGGTAAATGAAATTTTGTCATCTTGAATAGATTGTTGGAACCGTTCTATTTGCCAATCATCTATTTTTTCCACAACCTCACCGAGCATTGTCCTGGCAATACTTGATATATCATCACCTATACCATTTGCAATATAccc                       >  2:3960500/1‑150 (MQ=255)
                                                                                                                              aTCCGTTTGTATGGTAAATGAAATTTTGTCATCTTGAATAGATTGTTGGAACCGTTCTATTTGCCAATCATCTATTTTTTCCACAACCTCACCGAGCATTGTCCTGGCAATACTTGATATATCATCACCTATACCATTTGCAATATAccc                       >  2:2240126/1‑150 (MQ=255)
                                                                                                                                  gTTTGTATGGTAAATGAAATTTTGTCATCTTGAATAGATTGTTGGAACCGTTCTATTTGCCAATCATCTATTTTTTCCACAACCTCACCGAGCATTGTCCTGGCAATACTTGATATATCATCACCTATACCATTTGCAATATACCCAAAc                   >  2:3416101/1‑150 (MQ=255)
                                                                                                                                     tGTATGGTAAATGAAATTTTGTCATCTTGAATAGATTGTTGGAACCGTTCTATTTGCCAATCATCTATTTTTTCCACAACCTCACCGAGCATTGTCCTGGCAATACTTGATATATCATCACCTATACCATTTGCAATATACCCAAACAGt                >  2:2659753/1‑150 (MQ=255)
                                                                                                                                      gTATGGTAAATGAAATTTTGTCATCTTGAATAGATTGTTGGAACCGTTCTATTTGCCAATCATCTATTTTTTCCACAACCTCACCGAGCATTGTCCTGGCAATACTTGATATATCATCACCTATACCATTTGCAATATACCCAAACAGtt               <  2:1772757/150‑1 (MQ=255)
                                                                                                                                         tGGTAAATGAATTTTTGTCATCTTGAATAGATTGTTGGAACCGTTCTATTTGCCAATCATCTATTTTTTCCACAACCTCACCGAGCATTGTCCTGGCAATACTTGATATATCATCACCTATACCATTTGCAATATACCCAAACAGTTCtt            >  1:4491447/1‑150 (MQ=255)
                                                                                                                                         tGGTAAATGAATTTTTGTCATCTTGAATAGATTGTTGGAACCGTTCTATTTGCCAATCATCTATTTTTTCCACAACCTCACCGAGCATTGTCCTGGCAATACTTGATATATCATCACCTATACCATTTGCAATATACCCAAACAGTTCtt            <  2:3189365/150‑1 (MQ=255)
                                                                                                                                           gTAAATGAAATTTTGTCATCTTGAATAGATTGTTGGAACCGTTCTATTTGCCAATCATCTATTTTTTCCACAACCTCACCGAGCATTGTCCTGGCAATACTTGATATATCATCACCTATACCATTTGCAATATACCCAAACAGTTCTTTa          <  1:2736052/150‑1 (MQ=255)
                                                                                                                                            tAAATGAAATTTTGTCATCTTGAATAGATTGTTGGAACCGTTCTATTTGCCAATCATCTATTTTTTCCACAACCTCACCGAGCATTGTCCTGGCAATACTTGATATATCATCACCTATACCATTTGCAATATACCCAAACAGTTCTTTAg         >  2:3759637/1‑150 (MQ=255)
                                                                                                                                             aaaTGAAATTTTGTCATCTTGAATAGATTGTTGGAACCGTTCTATTTGCCAATCATCTATTTTTTCCACAACCTCACCGAGCATTGTCCTGGCAATACTTGATATATCATCACCTATACCATTTGCAATATACCCAAACAGTTCTTTAGt        >  2:1473039/1‑150 (MQ=255)
                                                                                                                                              aaTGAATTTTTGTCATCTTGAATAGATTGTTGGAACCGTTCTATTTGCCAATCATCTATTTTTTCCACAACCTCACCGAGCATTGTCCTGGCAATACTTGATATATCATCACCTATACCATTTGCAATATACCCAAACAGTTCTTTAGTg       <  1:2600707/150‑1 (MQ=255)
                                                                                                                                              aaTGAATTTTTGTCATCTTGAATAGATTGTTGGAACCGTTCTATTTGCCAATCATCTATTTTTTCCACAACCTCACCGAGCATTGTCCTGGCAATACTTGATATATCATCACCTATACCATTTGCAATATACCCAAACAGTTCTTTAGTg       <  2:3069528/150‑1 (MQ=255)
                                                                                                                                               aTGAAATTTTGTCATCTTGAATAGATTGTTGGAACCGTTCTATTTGCCAATCATCTATTTTTTCCACAACCTCACCGAGCATTGTCCTGGCAATACTTGATATATCATCACCTATACCATTTGCAATATACCCAAACAGTTCTTTAGTGa      >  2:3843759/1‑150 (MQ=255)
                                                                                                                                                tGAAATTTTGTCATCTTGAATAGATTGTTGGAACCGTTCTATTTGCCAATCATCTATTTTTTCCACAACCTCACCGAGCATTGTCCTGGCAATACTTGATATATCATCACCTATACCATTTGCAATATACCCAAACAGTTCTTTAGTGaa     >  2:2500353/1‑150 (MQ=255)
                                                                                                                                                 gAATTTTTGTCATCTTGAATAGATTGTTGGAACCGTTCTATTTGCCAATCATCTATTTTTTCCACAACCTCACCGAGCATTGTCCTGGCAATACTTGATATATCATCACCTATACCATTTGCAATATACCCAAACAGTTCTTTAGTGAAg    <  2:3791965/150‑1 (MQ=255)
                                                                                                                                                 gAAATTTTGTCATCTTGAATAGATTGTTGGAACCGTTCTATTTGCCAATCATCTATTTTTTCCACAACCTCACCGAGCATTGTCCTGGCAATACTTGATATATCATCACCTATACCATTTGCAATATACCCAAACAGTTCTTTAGTGAAg    <  1:3092219/150‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
AATATCGGTATTATCAAGAGAGCAACCGTAATTTTTGCTATTAATTGTCACAATAAACTGATAATTATTATTCCTACGTAGAATATGAGCACGCATGCCCGATAGCATACTATATTTTTCTGAATGATCCGTTTGTATGGTAAATGAAATTTTGTCATCTTGAATAGATTGTTGGAACCGTTCTATTTGCCAATCATCTATTTTTTCCACAACCTCACCGAGCATTGTCCTGGCAATACTTGATATATCATCACCTATACCATTTGCAATATACCCAAACAGTTCTTTAGTGAAGTT  >  CP009273/3625573‑3625869

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑