Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,695,997 C→A 19.9% E78* (GAG→TAG)  dppF ← dipeptide transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,695,9970CA19.9% 849.5 / 153.0 307E78* (GAG→TAG) dppFdipeptide transporter
Reads supporting (aligned to +/- strand):  ref base C (117/128);  new base A (32/30);  total (149/158)
Fisher's exact test for biased strand distribution p-value = 6.70e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 311 total aligned reads displayed.

CAGCAGCGGCTCTTCAAGAATTTGCCCGACTTTTTTACGCGGATTCAGCGAACCGTAAGGGTTCTGGAAGACGATCTGGATTTTCTGCCGACGCAGCTTCTGCGCCTGCGGATCGTGCTTAAGCAGATCCTGCCCCTGGTAATACAGCTCGCCACCGGTGGGCATTTCAATCATCGTCAGCAACCGACCGAGGGTCGATTTACCGCAGCCAGATTCGCCCACTACTGCCAGCGTTTTGCCACGTTCAAGGTTAAACGAAACGCCATCCAGCGCTTTAACCAGACGTTCCGGCGCGAA  >  CP009273/3695848‑3696144
                                                                                                                                                     |                                                                                                                                                   
cagcagCGGCTCTTCAAGAATTTGCCCGACTTTTTTACGCGGATTCAGCGAACCGTAAGGGTTCTGGAAGACGATCTGGATTTTCTGCCGACGCAGCTTCTGCGCCTGCGGATCGTGCTTAAGCAGATCCTGCCCCTGGTAATACAGCTc                                                                                                                                                     <  1:3345926/150‑1 (MQ=255)
cagcagCGGCTCTTCAAGAATTTGCCCGACTTTTTTACGCGGATTCAGCGAACCGTAAGGGTTCTGGAAGACGATCTGGATTTTCTGCCGACGCAGCTTCTGCGCCTGCGGATCGTGCTTAAGCAGATCCTGCCCCTGGTAATACAGCTc                                                                                                                                                     >  2:225699/1‑150 (MQ=255)
  gcagcGGCTCTTCAAGAATTTGCCCGACTTTTTTACGCGGATTCAGCGAACCGTAAGGGTTCTGGAAGACGATCTGGATTTTCTGCCGACGCAGCTTCTGCGCCTGCGGATCGTGCTTAAGCAGATCCTGCCCCTGGTAATACAGCTAGc                                                                                                                                                   <  1:3852119/150‑1 (MQ=255)
   cagcGGCTCTTCAAGAATTTGCCCGACTTTTTTACGCGGATTCAGCGAACCGTAAGGGTTCTGGAAGACGATCTGGATTTTCTGCCGACGCAGCTTCTGCGCCTGCGGATCGTGCTTAAGCAGATCCTGCCCCTGGTAATACAGCTAGcc                                                                                                                                                  <  2:203592/150‑1 (MQ=255)
    agcGGCTCTTCAAGAATTTGCCCGACTTTTTTACGCGGATTCAGCGAACCGTAAGGGTTCTGGAAGACGATCTGGATTTTCTGCCGACGCAGCTTCTGCGCCTGCGGATCGTGCTTAAGCAGATCCTGCCCCTGGTAATACAGCTCGCCa                                                                                                                                                 <  2:2965594/150‑1 (MQ=255)
    agcGGCTCTTCAAGAATTTGCCCGACTTTTTTACGCGGATTCAGCGAACCGTAAGGGTTCTGGAAGACGATCTGGATTTTCTGCCGACGCAGCTTCTGCGCCTGCGGATCGTGCTTAAGCAGATCCTGCCCCTGGTAATACAGCTCGCCa                                                                                                                                                 <  1:4253742/150‑1 (MQ=255)
    agcGGCTCTTCAAGAATTTGCCCGACTTTTTTACGCGGATTCAGCGAACCGTAAGGGTTCTGGAAGACGATCTGGATTTTCTGCCGACGCAGCTTCTGCGCCTGCGGATCGTGCTTAAGCAGATCCTGCCCCTGGTAATACAGCTAGCCa                                                                                                                                                 >  1:888555/1‑150 (MQ=255)
       ggCTCTTCAAGAATTTGCCCGACTTTTTTACGCGGATTCAGCGAACCGTAAGGGTTCTGGAAGACGATCTGGATTTTCTGCCGACGCAGCTTCTGCGCCTGCGGATCGTGCTTAAGCAGATCCTGCCCCTGGTAATACAGCTCGCCACCg                                                                                                                                              >  2:2841807/1‑150 (MQ=255)
        gCTCTTCAAGAATTTGCCCGACTTTTTTACGCGGATTCAGCGAACCGTAAGGGTTCTGGAAGACGATCTGGATTTTCTGCCGACGCAGCTTCTGCGCCTGCGGATCGTGCTTAAGCAGATCCTGCCCCTGGTAATACAGCTCGCCACCgg                                                                                                                                             <  1:2206007/150‑1 (MQ=255)
           ctTCAAGAATTTGCCCGACTTTTTTACGCGGATTCAGCGAACCGTAAGGGTTCTGGAAGACGATCTGGATTTTCTGCCGACGCAGCTTCTGCGCCTGCGGATCGTGCTTAAGCAGATCCTGCCCCTGGTAATACAGCTCGCCACCGGTgg                                                                                                                                          >  2:2237024/1‑150 (MQ=255)
           ctTCAAGAATTTGCCCGACTTTTTTACGCGGATTCAGCGAACCGTAAGGGTTCTGGAAGACGATCTGGATTTTCTGCCGACGCAGCTTCTGCGCCTGCGGATCGTGCTTAAGCAGATCCTGCCCCTGGTAATACAGCTCGCCACCGGTgg                                                                                                                                          <  2:2480723/150‑1 (MQ=255)
            ttCAAGAATTTGCCCGACTTTTTTACGCGGATTCAGCGAACCGTAAGGGTTCTGGAAGACGATCTGGATTTTCTGCCGACGCAGCTTCTGCGCCTGCGGATCGTGCTTAAGCAGATCCTGCCCCTGGTAATACAGCTCGCCACCGGTggg                                                                                                                                         >  1:2403839/1‑150 (MQ=255)
            ttCAAGAATTTGCCCGACTTTTTTACGCGGATTCAGCGAACCGTAAGGGTTCTGGAAGACGATCTGGATTTTCTGCCGACGCAGCTTCTGCGCCTGCGGATCGTGCTTAAGCAGATCCTGCCCCTGGTAATACAGCTCGCCACCGGTggg                                                                                                                                         >  2:106896/1‑150 (MQ=255)
              cAAGAATTTGCCCGACTTTTTTACGCGGATTCAGCGAACCGTAAGGGTTCTGGAAGACGATCTGGATTTTCTGCCGACGCAGCTTCTGCGCCTGCGGATCGTGCTTAAGCAGATCCTGCCCCTGGTAATACAGCTCGCCACCGGTGGGCa                                                                                                                                       >  1:3583526/1‑150 (MQ=255)
              cAAGAATTTGCCCGACTTTTTTACGCGGATTCAGCGAACCGTAAGGGTTCTGGAAGACGATCTGGATTTTCTGCCGACGCAGCTTCTGCGCCTGCGGATCGTGCTTAAGCAGATCCTGCCCCTGGTAATACAGCTAGCCACCGGTGGGCa                                                                                                                                       <  1:395917/150‑1 (MQ=255)
                aGAATTTGCCCGACTTTTTTACGCGGATTCAGCGAACCGTAAGGGTTCTGGAAGACGATCTGGATTTTCTGCCGACGCAGCTTCTGCGCCTGCGGATCGTGCTTAAGCAGATCCTGCCCCTGGTAATACAGCTCGCCACCGGTGGGCAtt                                                                                                                                     >  1:3115433/1‑150 (MQ=255)
                 gAATTTGCCCGACTTTTTTACGCGGATTCAGCGAACCGTAAGGGTTCTGGAAGACGATCTGGATTTTCTGCCGACGCAGCTTCTGCGCCTGCGGATCGTGCTTAAGCAGATCCTGCCCCTGGTCATACAGCTCGCCACCGGTGGGCAttt                                                                                                                                    >  2:1190826/1‑150 (MQ=255)
                 gAATTTGCCCGACTTTTTTACGCGGATTCAGCGAACCGTAAGGGTTCTGGAAGACGATCTGGATTTTCTGCCGACGCAGCTTCTGCGCCTGCGGATCGTGCTTAAGCAGATCCTGCCCCTGGTAATACAGCTAGCCACCGGTGGGCAttt                                                                                                                                    <  1:970024/150‑1 (MQ=255)
                  aaTTTGCCCGACTTTTTTACGCGGATTCAGCGAACCGTAAGGGTTCTGGAAGACGATCTGGATTTTCTGCCGACGCAGCTTCTGCGCCTGCGGATCGTGCTTAAGCAGATCCTGCCCCTGGTAATACAGCTAGCCACCGGTGGGCATTTc                                                                                                                                   <  2:1541609/150‑1 (MQ=255)
                     ttGCCCGACTTTTTTACGCGGATTCAGCGAACCGTAAGGGTTCTGGAAGACGATCTGGATTTTCTGCCGACGCAGCTTCTGCGCCTGCGGATCGTGCTTAAGCAGATCCTGCCCCTGGTAATACAGCTCGCCACCGGTGGGCATTTCAat                                                                                                                                <  2:4170108/150‑1 (MQ=255)
                      tGCCCGACTTTTTTACGCGGATTCAGCGAACCGTAAGGGTTCTGGAAGACGATCTGGATTTTCTGCCGACGCAGCTTCTGCGCCTGCGGATCGTGCTTAAGCAGATCCTGCCCCTGGTAATACAGCTCGCCACCGGTGGGCATTTCAatc                                                                                                                               <  1:1127275/150‑1 (MQ=255)
                      tGCCCGACTTTTTTACGCGGATTCAGCGAACCGTAAGGGTTCTGGAAGACGATCTGGATTTTCTGCCGACGCAGCTTCTGCGCCTGCGGATCGTGCTTAAGCAGATCCTGCCCCTGGTAATACAGCTCGCCACCGGTGGGCATTTCAatc                                                                                                                               >  1:957089/1‑150 (MQ=255)
                        cccGACTTTTTTACGCGGATTCAGCGAACCGTAAGGGTTCTGGAAGACGATCTGGATTTTCTGCCGACGCAGCTTCTGCGCCTGCGGATCGTGCTTAAGCAGATCCTGCCCCTGGTAATACAGCTCGCCACCGGTGGGCATTTCAatcat                                                                                                                             <  1:1282900/150‑1 (MQ=255)
                        cccGACTTTTTTACGCGGATTCAGCGAACCGTAAGGGTTCTGGAAGACGATCTGGATTTTCTGCCGACGCAGCTTCTGCGCCTGCGGATCGTGCTTAAGCAGATCCTGCCCCTGGTAATACAGCTCGCCACCGGTGGGCATTTCAatcat                                                                                                                             <  1:1910461/150‑1 (MQ=255)
                         ccGACTTTTTTACGCGGATTCAGCGAACCGTAAGGGTTCTGGAAGACGATCTGGATTTTCTGCCGACGCAGCTTCTGCGCCTGCGGATCGTGCTTAAGCAGATCCTGCCCCTGGTAATACAGCTAGCCACCGGTGGGCATTTCAatcatc                                                                                                                            <  2:3677233/150‑1 (MQ=255)
                          cGACTTTTTTACGCGGATTCAGCGAACCGTAAGGGTTCTGGAAGACGATCTGGATTTTCTGCCGACGCAGCTTCTGCGCCTGCGGATCGTGCTTAAGCAGATCCTGCCCCTGGTAATACAGCTCGCCACCGGTGGGCATATCAATCATCg                                                                                                                           >  1:2315854/1‑150 (MQ=255)
                           gACTTTTTTACGCGGATTCAGCGAACCGTAAGGGTTCTGGAAGACGATCTGGATTTTCTGCCGACGCAGCTTCTGCGCCTGCGGATCGTGCTTAAGCAGATCCTGCCCCTGGTAATACAGCTAGCCACCGGTGGGCATTTCAATCATCGt                                                                                                                          >  1:1469524/1‑150 (MQ=255)
                             cTTTTTTACGCGGATTCAGCGAACCGTAAGGGTTCTGGAAGACGATCTGGATTTTCTGCCGACGCAGCTTCTGCGCCTGCGGATCGTGCTTAAGCAGATCCTGCCCCTGGTACTACAGCTAGCCACCGGTGGGCATTTCAATCATCGTCa                                                                                                                        >  2:614189/1‑150 (MQ=255)
                              ttttttACGCGGATTCAGCGAACCGTAAGGGTTCTGGAAGACGATCTGGATTTTCTGCCGACGCAGCTTCTGCGCCTGCGGATCGTGCTTAAGCAGATCCTGCCCCTGGTAATACAGCTCGCCACCGGTGGGCATTTCAATCATCGTCAg                                                                                                                       <  1:4164851/150‑1 (MQ=255)
                                  ttccgcgGATTCAGCGAACCGTAAGGGTTCTGGAAGACGATCTGGATTTTCTGCCGACGCAGCTTCTGCGCCTGCGGATCGTGCTTAAGCAGATCCTGCCCCTGGTAATACAGCTCGCCACCGGTGGGCATTTCAATCATCGTCAGCAac                                                                                                                   <  2:2098593/147‑1 (MQ=255)
                                  ttACGCGGATTCAGCGAACCGTAAGGGTTCTGGAAGACGATCTGGATTTTCTGCCGACGCAGCTTCTGCGCCTGCGGATCGTGCTTAAGCAGATCCTGCCCCTGGTAATACAGCTCGCCACCGGTGGGCATTTCAATCATCGTCAGCAac                                                                                                                   <  2:2669269/150‑1 (MQ=255)
                                     cgcgGATTCAGCGAACCGTAAGGGTTCTGGAAGACGATCTGGATTTTCTGCCGACGCAGCTTCTGCGCCTGCGGATCGTGCTTAAGCAGATCCTGCCCCTGGTAATACAGCTAGCCACCGGTGGGCATTTCACTCATCGTCAGCAaccga                                                                                                                >  1:4077023/1‑150 (MQ=255)
                                      gcgGATTCAGCGAACCGTAAGGGTTCTGGAAGACGATCTGGATTTTCTGCCGACGCAGCTTCTGCGCCTGCGGATCGTGCTTAAGCAGATCCTGCCCCTGGTAATACAGCTCGCCACCGGTGGGCATTTCAATCATCGTCAGCAaccgac                                                                                                               <  1:2870201/150‑1 (MQ=255)
                                       cgGATTCAGCGAACCGTAAGGGTTCTGGAAGACGATCTGGATTTTCTGCCGACGCAGCTTCTGCGCCTGCGGATCGTGCTTAAGCAGATCCTGCCCCTGGTAATACAGCTCGCCACCGGTGGGCATTTCAATCATCGTCAGCAaccgacc                                                                                                              <  1:653462/150‑1 (MQ=255)
                                       cgGATTCAGCGAACCGTAAGGGTTCTGGAAGACGATCTGGATTTTCTGCCGACGCAGCTTCTGCGCCTGCGGATCGTGCTTAAGCAGATCCTGCCCCTGGTAATACAGCTCGCCACCGGTGGGCATTTCAATCATCGTCAGCAaccgacc                                                                                                              <  1:1056502/150‑1 (MQ=255)
                                       cgGATTCAGCGAACCGTAAGGGTTCTGGAAGACGATCTGGATTTTCTGCCGACGCAGCTTCTGCGCCTGCGGATCGTGCTTAAGCAGATCCTGCCCCTGGTAATACAGCTCGCCACCGGTGGGCATTTCAATCATCGTCAGCAaccgacc                                                                                                              <  2:186977/150‑1 (MQ=255)
                                         gATTCAGCGAACCGTAAGGGTTCTGGAAGACGATCTGGATTTTCTGCCGACGCAGCTTCTGCGCCTGCGGATCGTGCTTAAGCAGATCCTGCCCCTGGTAATACAGCTAGCCACCGGTGGGCATTTCAATCATCGTCAGCAAccgaccga                                                                                                            >  2:2795669/1‑150 (MQ=255)
                                            tCAGCGAACCGTAAGGGTTCTGGAAGACGATCTGGATTTTCTGCCGACGCAGCTTCTGCGCCTGCGGATCGTGCTTAAGCAGATCCTGCCCCTGGTAATACAGCTCGCCACCGGTGGGCATTTCAATCATCGTCAGCAACCGACCGAggg                                                                                                         >  2:4214286/1‑150 (MQ=255)
                                            tCAGCGAACCGTAAGGGTTCTGGAAGACGATCTGGATTTTCTGCCGACGCAGCTTCTGCGCCTGCGGATCGTGCTTAAGCAGATCCTGCCCCTGGTAATACAGCTCGCCACCGGTGGGCATTTCAATCATCGTCAGCAACCGACCGAggg                                                                                                         >  2:3092048/1‑150 (MQ=255)
                                              aGCGAACCGTAAGGGTTCTGGAAGACGATCTGGATTTTCTGCCGACGCAGCTTCTGCGCCTGCGGATCGTGCTTAAGCAGATCCTGCCCCTGGTAATACAGCTCGCCACCGGTGGGAATTTCAATCAGCGTCAGCAACCGACCGAGGGTc                                                                                                       <  1:926039/150‑1 (MQ=255)
                                              aGCGAACCGTAAGGGTTCTGGAAGACGATCTGGATTTTCTGCCGACGCAGCTTCTGCGCCTGCGGATCGTGCTTAAGCAGATCCTGCCCCTGGTAATACAGCTAGCCACCGGTGGGCATTTCAATCATCGTCAGCAACCGACCGAGGGTc                                                                                                       <  2:3522306/150‑1 (MQ=255)
                                                cGAACCGTAAGGGTTCTGGAAGACGATCTGGATTTTCTGCCGACGCAGCTTCTGCGCCTGCGGATCGTGCTTAAGCAGATCCTGCCCCTGGTAATACAGCTCGCCACCGGTGGGCATTTCAATCATCGTCAGCAACCGACCGAGGGTCGa                                                                                                     >  2:4398273/1‑150 (MQ=255)
                                                cGAACCGTAAGGGTTCTGGAAGACGATCTGGATTTTCTGCCGACGCAGCTTCTGCGCCTGCGGATCGTGCTTAAGCAGATCCTGCCCCTGGTAATACAGCTCGCCACCGGTGGGCATTTCAATCATCGTCAGCAACCGACCGAGGGTCGa                                                                                                     >  2:2124059/1‑150 (MQ=255)
                                                 gAACCGTAAGGGTTCTGGAAGACGATCTGGATTTTCTGCCGACGCAGCTTCTGCGCCTGCGGATCGTGCTTAAGCAGATCCTGCCCCTGGTAATACAGCTAGCCACCGGTGGGCATTTCAATCATCGTCAGCAACCGACCGAGGGTCGAt                                                                                                    >  1:400807/1‑150 (MQ=255)
                                                   aCCGTAAGGGTTCTGGAAGACGATCTGGATTTTCTGCCGACGCAGCTTCTGCGCCTGCGGATCGTGCTTAAGCAGATCCTGCCCCTGGTAATACAGCTCGCCACCGGTGGGCATTTCAATCATCGTCAGCAACCGACCGAGGGTCGAttt                                                                                                  >  1:1352548/1‑150 (MQ=255)
                                                    ccGTAAGGGTTCTGGAAGACGATCTGGATTTTCTGCCGACGCAGCTTCTGCGCCTGCGGATCGTGCTTAAGCAGATCCTGCCCCTGGTAATACAGCTCGCCACCGGTGGGCATTTCAATCATCGTCAGCAACCGACCGAGGGTCGATTTa                                                                                                 >  1:1660061/1‑150 (MQ=255)
                                                         aGGGTTCTGGAAGACGATCTGGATTTTCTGCCGACGCAGCTTCTGCGCCTGCGGATCGTGCTTAAGCAGATCCTGCCCCTGGTAATACAGCTCGCCACCGGTGGGCATTTCAATCATCGTCAGCAACCGACCGAGGGTCGATTTACCGCa                                                                                            >  1:2620241/1‑150 (MQ=255)
                                                            gTTCTGGAAGACGATCTGGATTTTCTGCCGACGCAGCTTCTGCGCCTGCGGATCGTGCTTAAGCAGATCCTGCCCCTGGTAATACAGCTCGCCACCGGTGGGCATTTCAATCATCGTCAGCAACCGACCGAGGGTCGATTTACCGCAGcc                                                                                         >  2:456918/1‑150 (MQ=255)
                                                            gTTCTGGAAGACGATCTGGATTTTCTGCCGACGCAGCTTCTGCGCCTGCGGATCGTGCTTAAGCAGATCCTGCCCCTGGTAATACAGCTCGCCACCGGTGGGCATTTCAATCATCGTCAGCAACCGACCGAGGGTCGATTTACCGCAGcc                                                                                         <  1:2237572/150‑1 (MQ=255)
                                                               cTGGAAGACGATCTGGATTTTCTGCCGACGCAGCTTCTGCGCCTGCGGATCGTGCTTAAGCAGATCCTGCCCCTGGTAATACAGCTCGCCACCGGTGGGCATTTCAATCATCGTCAGCAACCGACCGAGGGTCGATTTACCGCAGCCAGa                                                                                      >  2:4308296/1‑150 (MQ=255)
                                                                tGGAAGACGATCTGGATTTTCTGCCGACGCAGCTTCTGCGCCTGCGGATCGTGCTTAAGCAGATCCTGCCCCTGGTAATACAGCTCGCCACCGGTGGGCATTTCAATCATCGTCAGCAACCGACCGAGGGTCGATTTACCGCAGCCAGAt                                                                                     <  2:4175971/150‑1 (MQ=255)
                                                                 ggAAGACGATCTGGATTTTCTGCCGACGCAGCTTCTGCGCCTGCGGATCGTGCTTAAGCAGATCCTGCCCCTGGTAATACAGCTCGCCACCGGTGGGCATTTCAATCATCGTCAGCAACCGACCGAGGGTCGATTTACCGCAGCCAGAtt                                                                                    >  2:3341313/1‑150 (MQ=255)
                                                                 ggAAGACGATCTGGATTTTCTGCCGACGCAGCTTCTGCGCCTGCGGATCGTGCTTAAGCAGATCCTGCCCCTGGTAATACAGCTCGCCACCGGTGGGCATTTCAATCATCGTCAGCAACCGACCGAGGGTCGATTTACCGCAGCCAGAtt                                                                                    >  1:3895229/1‑150 (MQ=255)
                                                                       cGATCTGGATTTTCTGCCGACGCAGCTTCTGCGCCTGCGGATCGTGCTTAAGCAGATCCTGCCCCTGGTAATACAGCTAGCCACCGGTGGGCATTTCAATCATCGTCAGCAACCGACCGAGGGTCGATTTACCGCAGCCAGATTCGCCCa                                                                              >  1:1408313/1‑150 (MQ=255)
                                                                       cGATCTGGATTTTCTGCCGACGCAGCTTCTGCGCCTGCGGATCGTGCTTAAGCAGATCCTGCCCCTGGTAATACAGCTAGCCACCGGTGGGCATTTCAATCATCGTCAGCAACCGACCGAGGGTCGATTTACCGCAGCCAGATTCGCCCa                                                                              >  1:3663784/1‑150 (MQ=255)
                                                                        gATCTGGATTTTCTGCCGACGCAGCTTCTGCGCCTGCGGATCGTGCTTAAGCAGATCCTGCCCCTGGTAATACAGCTCGCCACCGGTGGGCATTTCAATCATCGTCAGCAACCGACCGAGGGTCGATTTACCGCAGCCAGATTCGCCCac                                                                             >  1:2440586/1‑150 (MQ=255)
                                                                        gATCTGGATTTTCTGCCGACGCAGCTTCTGCGCCTGCGGATCGTGCTTAAGCAGATCCTGCCCCTGGTAATACAGCTAGCCACCGGTGGGCATTTCAATCATCGTCAGCAACCGACCGAGGGTCGATTTACCGCAGCCAGATTCGCCCac                                                                             >  1:470841/1‑150 (MQ=255)
                                                                        gATCTGGATTTTCTGCCGACGCAGCTTCTGCGCCTGCGGATCGTGCTTAAGCAGATCCTGCCCCTGGTAATACAGCTAGCCACCGGTGGGCATTTCAATCATCGTCAGCAACCGACCGAGGGTCGATTTACCGCAGCCAGATTCGCCCac                                                                             >  1:4503874/1‑150 (MQ=255)
                                                                          tCTGGATTTTCTGCCGACGCAGCTTCTGCGCCTGCGGATCGTGCTTAAGCAGATCCTGCCCCTGGTAATACAGCTCGCCACCGGTGGGCATTTCAATCATCGTCAGCAACCGACCGAGGGTCGATTTACCGCAGCCAGATTCGCCCacta                                                                           >  1:804043/1‑150 (MQ=255)
                                                                          tCTGGATTTTCTGCCGACGCAGCTTCTGCGCCTGCGGATCGTGCTTAAGCAGATCCTGCCCCTGGTAATACAGCTCGCCACCGGTGGGCATTTCAATCATCGTCAGCAACCGACCGAGGGTCGATTTACCGCAGCCAGATTCGCCCacta                                                                           <  1:4228795/150‑1 (MQ=255)
                                                                           cTGGATTTTCTGCCGACGCAGCTTCTGCGCCTGCGGATCGTGCTTAAGCAGATCCTGCCCCTGGTAATACAGCTCGCCACCGGTGGGCATTTCAATCATCGTCAGCAACCGACCGAGGGTCGATTTACCGCAGCCAGATTCGCCCactac                                                                          >  2:166689/1‑150 (MQ=255)
                                                                           cTGGATTTTCTGCCGACGCAGCTTCTGCGCCTGCGGATCGTGCTTAAGCAGATCCTGCCCCTGGTAATACAGCTCGCCACCGGTGGGCATTTCAATCATCGTCAGCAACCGACCGAGGGTCGATTTACCGCAGCCAGATTCGCCCactac                                                                          <  1:1022597/150‑1 (MQ=255)
                                                                               aTTTTCTGCCGACGCAGCTTCTGCGCCTGCGGATCGTGCTTAAGCAGATCCTGCCCCTGGTAATACAGCTCGCCACCGGTGGGCATTTCAATCATCGTCAGCAACCGACCGAGGGTCGATTTACCGCAGCCAGATTCGCCCACTACTGcc                                                                      <  1:1568324/150‑1 (MQ=255)
                                                                                ttttCTGCCGACGCAGCTTCTGCGCCTGCGGATCGTGCTTAAGCAGATCCTGCCCCTGGTAATACAGCTCGCCACCGGTGGGCATTTCAATCATCGTCAGCAACCGACCGAGGGTCGATTTACCGCAGCCAGATTCGCCCACTACTGCCa                                                                     <  1:77222/150‑1 (MQ=255)
                                                                                  ttCTGCCGACGCAGCTTCTGCGCCTGCGGATCGTGCTTAAGCAGATCCTGCCCCTGGTAATACAGCTCGCCACCGGTGGGCATTTCAATCATCGTCAGCAACCGACCGAGGGTCGATTTACCGCAGCCAGATTCGCCCACTACTGCCAGc                                                                   <  1:1967493/150‑1 (MQ=255)
                                                                                   tCTGCCGACGCAGCTTCTGCGCCTGCGGATCGTGCTTAAGCAGATCCTGCCCCTGGTAATACAGCTCGCCACCGGTGGGCATTTCAATCATCGTCAGCAACCGACCGAGGGTCGATTTACCGCAGCCAGATTCGCCCACTACTGCCAGCg                                                                  >  1:33235/1‑150 (MQ=255)
                                                                                      gCCGACGCAGCTTCTGCGCCTGCGGATCGTGCTTAAGCAGATCCTGCCCCTGGTAATACAGCTCGCCACCGGTGGGCATTTCAATCATCGTCAGCAACCGACCGAGGGTCGATTTACCGCAGCCAGATTCGCCCACTACTGCCAGCGttt                                                               <  2:3759686/150‑1 (MQ=255)
                                                                                      gCCGACGCAGCTTCTGCGCCTGCGGATCGTGCTTAAGCAGATCCTGCCCCTGGTAATACAGCTCGCCACCGGTGGGCATTTCAATCATCGTCAGCAACCGACCGAGGGTCGATTTACCGCAGCCAGATTCGCCCACTACTGCCAGCGttt                                                               >  1:215907/1‑150 (MQ=255)
                                                                                      gCCGACGCAGCTTCTGCGCCTGCGGATCGTGCTTAAGCAGATCCTGCCCCTGGTAATACAGCTCGCCACCGGTGGGCATTTCAATCATCGTCAGCAACCGACCGAGGGTCGATTTACCGCAGCCAGATTCGCCCACTACTGCCAGCGttt                                                               <  2:2319248/150‑1 (MQ=255)
                                                                                       ccGACGCAGCTTCTGCGCCTGCGGATCGTGCTTAAGCAGATCCTGCCCCTGGTAATACAGCGAGCCACCGGTGGGCATTTCAATCATCGTCAGCAACCGACCGAGGGTCGATTTACCGCAGCCAGATTCGCCCACTACTGCCAGCGtttt                                                              <  2:2264631/150‑1 (MQ=255)
                                                                                        cGACGCAGCTTCTGCGCCTGCGGATCGTGCTTAAGCAGATCCTGCCCCTGGTAATACAGCTCGCCACCGGTGGGCATTTCAATCATCGTCAGCAACCGACCGAGGGTCGATTTACCGCAGCCAGATTCGCCCACTACTGCCAGCGTTTTg                                                             >  2:1404251/1‑150 (MQ=255)
                                                                                            gCAGCTTCTGCGCCTGCGGATCGTGCTTAAGCAGATCCTGCCCCTGGTAATACAGCTCGCCACCGGTGGGCATTTCAATCATCGTCAGCAACCGACCGAGGGTCGATTTACCGCAGCCAGATTCGCCCACTACTGCCAGCGTTTTGCCAc                                                         <  2:4162980/150‑1 (MQ=255)
                                                                                                         cTGCGGATCGTGCTTAAGCAGATCCTGCCCCTGGTAATACAGCTCGCCACCGGTGGGCATTTCAATCATCGTCAGCAACCGACCGAGGGTCGATTTACCGCAGCCAGATTCGCCCACTACTGCCAGCGTTTTGCCACGTTCAAGGTTaaa                                            >  2:701312/1‑150 (MQ=255)
                                                                                                         cTGCGGATCGTGCTTAAGCAGATCCTGCCCCTGGTAATACAGCTAGCCACCGGTGGGCATTTCAATCATCGTCAGCAACCGACCGAGGGTCGATTTACCGCAGCCAGATTCGCCCACTACTGCCAGCGTTTTGCCACGTTCAAGGTTaaa                                            >  1:576012/1‑150 (MQ=255)
                                                                                                         cTGCGGATCGTGCTTAAGCAGATCCTGCCCCTGGTAATACAGCTAGCCACCGGTGGGCATTTCAATCATCGTCAGCAACCGACCGAGGGTCGATTTACCGCAGCCAGATTCGCCCACTACTGCCAGCGTTTTGCCACGTTCAAGGTTaaa                                            >  2:1232066/1‑150 (MQ=255)
                                                                                                          tGCGGATCGTGCTTAAGCAGATCCTGCCCCTGGTAATACAGCTCGCCACCGGTGGGCATTTCAATCATCGTCAGCAACCGACCGAGGGTCGATTTACCGCAGCCAGATTCGCCCACTACTGCCAGCGTTTTGCCACGTTCAAGGTTaaac                                           <  2:1360160/150‑1 (MQ=255)
                                                                                                                tCGTGCTTAAGCAGATCCTGCCCCTGGTAATACAGCTCGCCACCGGTGGGCATTTCAATCATCGTCAGCAACCGACCGAGGGTCGATTTACCGCAGCCAGATTCGCCCACTACTGCCAGCGTTTTGCCACGTTCAAGGTTaaacgaaacg                                     <  2:2692067/150‑1 (MQ=255)
                                                                                                                  gTGCTTAAGCAGATCCTGCCCCTGGTAATACAGCTCGCCACCGGTGGGCATTTCAATCATCGTCAGCAACCGACCGAGGGTCGATTTACCGCAGCCAGATTCGCCCACTACTGCCAGCGTTTTGCCACGTTCAAGGTTAAACGAAACGcc                                   >  2:2551834/1‑150 (MQ=255)
                                                                                                                  gTGCTTAAGCAGATCCTGCCCCTGGTAATACAGCTCGCCACCGGTGGGCATTTCAATCATCGTCAGCAACCGACCGAGGGTCGATTTACCGCAGCCAGATTCGCCCACTACTGCCAGCGTTTTGCCACGTTCAAGGTTAAACGAAACGcc                                   <  1:1759164/150‑1 (MQ=255)
                                                                                                                   tGCTTAAGCAGATCCTGCCCCTGGTAATACAGCTCGCCACCGGTGGGCATTTCAATCATCGTCAGCAACCGACCGAGGGTCGATTTACCGCAGCCAGATTCGCCCACTACTGCCAGCGTTTTGCCACGTTCAAGGTTAAACGAAACGCCa                                  <  1:4380671/150‑1 (MQ=255)
                                                                                                                   tGCTTAAGCAGATCCTGCCCCTGGTAATACAGCTCGCCACCGGTGGGAATTTCAATCATCGTCAGCAACCGACCGAGGGTCGATTTACCGCAGCCAGATTCGCCCACTACTGCCAGCGTTTTGCCACGTTCAAGGTTAAACGAAACGCCa                                  <  1:1446521/150‑1 (MQ=255)
                                                                                                                      ttAAGCAGATCCTGCCCCTGGTAATACAGCTCGCCACCGGTGGGCATTTCAATCATCGTCAGCAACCGACCGAGGGTCGATTTACCGCAGCCAGATTCGCCCACTACTGCCAGCGTTTTGCCACGTTCAAGGTTAAACGAAACGCCATcc                               <  1:2604160/150‑1 (MQ=255)
                                                                                                                       tAAGCAGATCCTGCCCCTGGTAATACAGCTCGCCACCGGTGGGCATTTCAATCATCGTCAGCAACCGACCGAGGGTCGATTTACCGCAGCCAGATTCGCCCACTACTGCCAGCGTTTTGCCACGTTCAAGGTTAAACGAAACGCCATCCa                              <  2:2928671/150‑1 (MQ=255)
                                                                                                                       tAAGCAGATCCTGCCCCTGGTAATACAGCTAGCCACCGGTGGGCATTTCAATCATCGTCAGCAACCGACCGAGGGTCGATTTACCGCAGCCAGATTCGCCCACTACTGCCAGCGTTTTGCCACGTTCAAGGTTAAACGAAACGCCATCCa                              <  2:3173395/150‑1 (MQ=255)
                                                                                                                          gCAGATCCTGCCCCTGGTAATACAGCTCGCCACCGGTGGGCATTTCATTCATCGTCAGCAACCGACCGAGGGTCGATTTACCGCAGCCAGATTCGCCCACTACTGCCAGCGTTTTGCCACGTTCAAGGTTAAACGAAACGCCATCCAgcg                           <  1:3397379/150‑1 (MQ=255)
                                                                                                                          gCAGATCCTGCCCCTGGTAATACAGCTCGCCACCGGTGGGCATTTCAATCATCGTCAGCAACCGACCGAGGGTCGATTTACCGCAGCCAGATTCGCCCACTACTGCCAGCGTTTTGCCACGTTCAAGGTTAAACGAAACGCCATCCAgcg                           <  1:3708252/150‑1 (MQ=255)
                                                                                                                            aGATCCTGCCCCTGGTAATACAGCTCGCCACCGGTGGGCATTTCAATCATCGTCAGCAACCGACCGAGGGTCGATTTACCGCAGCCAGATTCGCCCACTACTGCCAGCGTTTTGCCACGTTCAAGGTTAAACGAAACGCCATCCAGCGCt                         <  2:4538003/150‑1 (MQ=255)
                                                                                                                             gATCCTGCCCCTGGTAATACAGCTCGCCACCGGTGGGCATTTCAATCATCGTCAGCAACCGACCGAGGGTCGATTTACCGCAGCCAGATTCGCCCACTACTGCCAGCGTTTTGCCACGTTCAAGGTTAAACGAAACGCCATCCAGCGCtt                        >  1:2833059/1‑150 (MQ=255)
                                                                                                                               tCCTGCCCCTGTTAATACAGCTCGCCACCGGTGGGAATTTCAATCATCGTCAGCAACCGACCGAGGGTCGATTTACCGCAGCCAGATTCGCCCACTACTGCCAGCGTTTTGCCACGTTCAAGGTTAAACGAAACGCCATCCAGCGCTTTa                      <  1:1146154/150‑1 (MQ=255)
                                                                                                                                 cTGCCCCTGGTAATACAGCTAGCCACCGGTGGGCATTTCAATCATCGTCAGCAACCGACCGAGGGTCGATTTACCGCAGCCAGATTCGCCCACAACTGCCAGCGTTTTGCCACGTTCAAGGTTAAACGAAACGCCATCCAGCGCTTTAAc                    >  1:3042567/1‑150 (MQ=255)
                                                                                                                                  tGCCCCTGGTAATACAGCTCGCCACCGGTGGGCATTTCAATCATCGTCAGCAACCGACCGAGGGTCGATTTACCGCAGCCAGATTCGCCCACTACTGCCAGCGTTTTGCCACGTTCAAGGTTAAACGAAACGCCATCCAGCGCTTTAAcc                   >  1:3215123/1‑150 (MQ=255)
                                                                                                                                    ccccTGGTAATACAGCTCGCCACCGGTGGGCATTTCAATCATCGTCAGCAACCGACCGAGGGTCGATTTACCGCAGCCAGATTCGCCCACTACTGCCAGCGTTTTGCCACGTTCAAGGTTAAACGAAACGCCATCCAGCGCTTTAACCAg                 <  2:1998433/150‑1 (MQ=255)
                                                                                                                                    ccccTGGTAATACAGCTAGCCACCGGTGGGCATTTCCATCATCGTCAGCAACCGACCGAGGGTCGATTTACCGCAGCCAGATTCGCCCACTACTGCCAGCGTTTTGCCACGTTCAAGGTTAAACGAAACGCCATCCAGCGCTTTAACCAg                 <  2:177622/150‑1 (MQ=255)
                                                                                                                                         ggTAATACAGCTCGCCACCGGTGGGCATTTCAATCATCGTCAGCAACCGACCGAGGGTCGATTTACCGCAGCCAGATTCGCCCACTACTGCCAGCGTTTTGCCACGTTCAAGGTTAAACGAAACGCCATCCAGCGCTTTAACCAGACGtt            <  2:3892986/150‑1 (MQ=255)
                                                                                                                                           tAATACAGCTCGCCACCGGTGGGCATTTCAATCATCGTCAGCAACCGACCGAGGGTCGATTTACCGCAGCCAGATTCGCCCACTACTGCCAGCGTTTTGCCACGTTCAAGGTTAAACGAAACGCCATCCAGCGCTTTAACCAGACGTTcc          <  1:628492/150‑1 (MQ=255)
                                                                                                                                            aaTACAGCTAGCCACCGGTGGGCATTTCAATCATCGTCAGCAACCGACCGAGGGTCGATTTACCGCAGCCAGATTCGCCCACTACTGCCAGCGTTTTGCCACGTTCAAGGTTAAACGAAACGCCATCCAGCGCTTTAACCAGACGTTCCg         <  2:1469524/150‑1 (MQ=255)
                                                                                                                                              tACAGCTCGCCACCGGTGGGCATTTCAATCATCGTCAGCAACCGACCGAGGGTCGATTTACCGCAGCCAGATTCGCCCACTACTGCCAGCGTTTTGCCACGTTCAAGGTTAAACGAAACGCCATCCAGCGCTTTAACCAGACGTTCCGgc       >  2:3438973/1‑150 (MQ=255)
                                                                                                                                              tACAGCTCGCCACCGGTGGGCATTTCAATCATCGTCAGCAACCGACCGAGGGTCGATTTACCGCAGCCAGATTCGCCCACTACTGCCAGCGTTTTGCCACGTTCAAGGTTAAACGAAACGCCATCCAGCGCTTTAACCAGACGTTCCGgc       >  1:4327426/1‑150 (MQ=255)
                                                                                                                                                cAGCTCGCCACCGGTGGGCATTTCAATCATCGTCAGCAACCGACCGAGGGTCGATTTACCGCAGCCAGATTCGCCCACTACTGCCAGCGTTTTGCCACGTTCAAGGTTAAACGAAACGCCATCCAGCGCTTTAACCAGACGTTCCGgcgc     >  1:2282134/1‑150 (MQ=255)
                                                                                                                                                   cTCGCCACCGGTGGGCATTTCAATCATCGTCAGCAACCGACCGAGGGTCGATTTACCGCAGCCAGATTCGCCCACTACTGCCAGCGTTTTGCCACGTTCAAGGTTAAACGAAACGCCATCCAGCGCTTTAACCAGACGTTCCGGCGCGaa  >  2:4059162/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                   
CAGCAGCGGCTCTTCAAGAATTTGCCCGACTTTTTTACGCGGATTCAGCGAACCGTAAGGGTTCTGGAAGACGATCTGGATTTTCTGCCGACGCAGCTTCTGCGCCTGCGGATCGTGCTTAAGCAGATCCTGCCCCTGGTAATACAGCTCGCCACCGGTGGGCATTTCAATCATCGTCAGCAACCGACCGAGGGTCGATTTACCGCAGCCAGATTCGCCCACTACTGCCAGCGTTTTGCCACGTTCAAGGTTAAACGAAACGCCATCCAGCGCTTTAACCAGACGTTCCGGCGCGAA  >  CP009273/3695848‑3696144

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑