Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,700,154 G→T 18.4% L304L (CTC→CTA)  dppA ← dipeptide transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,700,1540GT18.4% 743.1 / 110.1 255L304L (CTC→CTA) dppAdipeptide transporter
Reads supporting (aligned to +/- strand):  ref base G (100/107);  new base T (24/24);  total (124/131)
Fisher's exact test for biased strand distribution p-value = 8.74e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 261 total aligned reads displayed.

TAGGTGTAGTCCTGAACGTCGTCGTTATAGCCCCACATGGTTGGCGGGATCAGGTTTTTCGCTGATACGCCCGCGCCCTGATAAACCGCTTTGATGATCGCGTCTTTGTTCACCGCGTAGGTCAGAGCCTGGCGAACTTTCACGTCATCGAGTGGTTTTTTCTGCACGTTATACGAGAGATAACCGACGTTCAGCCCCGGCATTTCCATCAGATTGATGGATTTATCCTGCTTCATGCGAGCGATATCTGCCGGGTTCGGGTACGGCATCACCTGGCATTCATTCTTCTGCAATTTCGC  >  CP009273/3700005‑3700303
                                                                                                                                                     |                                                                                                                                                     
tAGGTGTAGTCCTGAACGTCGTCGTTATAGCCCCACATGGTTGGCGGGATCAGGTTTTTCGCTGATACGCCCGCGCCCTGATAAACCGCTTTGATGATCGCGTCTTTGTTCACCGCGTAGGTCAGAGCCTGGCGAACTTTCACGTCATCg                                                                                                                                                       >  1:4224653/1‑150 (MQ=255)
tAGGTGTAGTCCTGAACGTCGTCGTTATAGCCCCACATGGTTGGCGGGATCAGGTTTTTCGCTGATACGCCCGCGCCCTGATAAACCGCTTTGATGATCGCGTCTTTGTTCACCGCGTAGGTCAGAGCCTGGCGAACTTTCACGTCATCg                                                                                                                                                       >  1:414170/1‑150 (MQ=255)
tAGGTGTAGTCCTGAACGTCGTCGTTATAGCCCCACATGGTTGGCGGGATCAGGTTTTTCGCTGATACGCCCGCGCCCTGATAAACCGCTTTGATGATCGCGTCTTTGTTCACCGCGTAGGTCAGAGCCTGGCGAACTTTCACGTCATCg                                                                                                                                                       <  2:669938/150‑1 (MQ=255)
 aGGTGTAGTCCTGAACGTCGTCGTTATAGCCCCACATGGTTGGCGGGATCAGGTTTTTCGCTGATACGCCCGCGCCCTGATAAACCGCTTTGATGATCGCGTCTTTGTTCACCGCGTAGGTCAGAGCCTGGCGAACTTTCACGTCATCGa                                                                                                                                                      >  1:1380000/1‑150 (MQ=255)
  ggTGTAGTCCTGAACGTCGTCGTTATAGCCCCACATGGTTGGCGGGATCAGGTTTTTCGCTGATACGCCCGCGCCCTGATAAACCGCTTTGATGATCGCGTCTTTGTTCACCGCGTAGGTCAGAGCCTGGCGAACTTTCACGTCATCGAg                                                                                                                                                     >  2:203617/1‑150 (MQ=255)
  ggTGTAGTCCTGAACGTCGTCGTTAGAGCCCCACAGGGTTGGCGGGATCAGGTTTTTCGCTGATACGCCCGCGCCCTGATAAACCGCTTTGATGATCGCGTCTTTGTTCACCGCGTAGGTCAGAGCCTGGCGAACTTTCACGTCATCGAg                                                                                                                                                     <  2:3140263/150‑1 (MQ=255)
     gtAGTCCTGAACGTCGTCGTTATAGCCCCACATGGTTGGCGGGATCAGGTTTTTCGCTGATACGCCCGCGCCCTGATAAACCGCTTTGATGATCGCGTCTTTGTTCACCGCGTAGGTCAGAGCCTGGCGAACTTTCACGTCATCGAGTgg                                                                                                                                                  >  1:521993/1‑150 (MQ=255)
       aGTCCTGAACGTCGTCGTTATAGCCCCACATGGTTGGCGGGATCAGGTTTTTCGCTGATACGCCCGCGCCCTGATAAACCGCTTTGATGATCGCGTCTTTGTTCACCGCGTAGGTCAGAGCCTGGCGAACTTTCACGTCATCTAGTGGtt                                                                                                                                                >  1:1210858/1‑150 (MQ=255)
       aGTCCTGAACGTCGTCGTTATAGCCCCACATGGTTGGCGGGATCAGGTTTTTCGCTGATACGCCCGCGCCCTGATAAACCGCTTTGATGATCGCGTCTTTGTTCACCGCGTAGGTCAGAGCCTGGCGAACTTTCACGTCATCTAGTGGtt                                                                                                                                                >  1:3828778/1‑150 (MQ=255)
       aGTCCTGAACGTCGTCGTTATAGCCCCACATGGTTGGCGGGATCAGGTTTTTCGCTGATACGCCCGCGCCCTGATAAACCGCTTTGATGATCGCGTCTTTGTTCACCGCGTAGGTCAGAGCCTGGCGAACTTTCACGTCATCGAGTGGtt                                                                                                                                                <  1:3015644/150‑1 (MQ=255)
        gTCCTGAACGTCGTCGTTATAGCCCCACATGGTTGGCGGGATCAGGTTTTTCGCTGATACGCCCGCGCCCTGATAAACCGCTTTGATGATCGCGTCTTTGTTCACCGCGTAGGTCAGAGCCTGGCGAACTTTCACGTCATCGAGTGGttt                                                                                                                                               <  2:1468930/150‑1 (MQ=255)
         tCCTGAACGTCGTCGTTATAGCCCCACATGGTTGGCGGGATCAGGTTTTTCGCTGATACGCCCGCGCCCTGATAAACCGCTTTGATGATCGCGTCTTTGTTCACCGCGTAGGTCAGAGCCTGGCGAACTTTCACGTCATCTAGTGGtttt                                                                                                                                              >  1:4447883/1‑150 (MQ=255)
         tCCTGAACGTCGTCGTTATAGCCCCACATGGTTGGCGGGATCAGGTTTTTCGCTGATACGCCCGCGCCCTGATAAACCGCTTTGATGATCGCGTCTTTGTTCACCGCGTAGGTCAGAGCCTGGCGAACTTTCACGTCATCGAGTGGtttt                                                                                                                                              <  2:1478363/150‑1 (MQ=255)
           cTGAACGTCGTCGTTATAGCCCCACATGGTTGGCGGGATCAGGTTTTTCGCTGATACGCCCGCGCCCTGATAAACCGCTTTGATGATCGCGTCTTTGTTCACCGCGTAGGTCAGAGCCTGGCGAACTTTCACGTCATCGAGTGGtttttt                                                                                                                                            >  2:2198486/1‑150 (MQ=255)
            tGAACGTCGTCGTTATAGCCCCACATGGTTTGCGGGATCAGGTTTTTCGCTGATACGCCCGCGCCCTGATAAAACGCTTTGAAGATCGCGTCTTTTTTCACCGCGTAGTTCAGAGCCTGGCGAACATTCACGTCATCGAGTGGtttttta                                                                                                                                           >  1:3539587/1‑149 (MQ=255)
               aCGTCGTCGTTATAGCCCCACATGGTTGGCGGGATCAGGTTTTTCGCTGATACGCCCGCGCCCTGATAAACCGCTTTGATGATCGCGTCTTTGTTCACCGCGTAGGTCAGAGCCTGGCGAACTTTCACGTCATCGAGTGGTTTTTTCTGc                                                                                                                                        <  2:606310/150‑1 (MQ=255)
                cgtcgtcgtTATAGCCCCACATGGTTGGCGGGATCAGGTTTTTCGCTGATACGCCCGCGCCCTGATAAACCGCTTTGATGATCGCGTCTTTGTTCACCGCGTAGGTCAGAGCCTGGCGAACTTTCACGTCATCGAGTGGTTTTTTCTGCa                                                                                                                                       <  2:4428790/150‑1 (MQ=255)
                    gtcgtTATAGCCCCACATGGTTGGCGGGATCAGGTTTTTCGCTGATACGCCCGCGCCCTGATAAACCGCTTTGATGATCGCGTCTTTGTTCACCGCGTAGGTCAGAGCCTGGCGAACTTTCACGTCATCGAGTGGTTTTTTCTGCACGtt                                                                                                                                   <  2:1877287/150‑1 (MQ=255)
                      cgtTATAGCCCCACATGGTTGGCGGGATCAGGTTTTTCGCTGATACGCCCGCGCCCTGATAAACCGCTTTGATGATCGCGTCTTTGTTCACCGCGTAGGTCAGAGCCTGGCGAACTTTCACGTCATCGAGTGGTTTTTTCTGCACGTtat                                                                                                                                 <  2:2341239/150‑1 (MQ=255)
                       gtTATAGCCCCACATGGTTGGCGGGATCAGGTTTTTCGCTGATACGCCCGCGCCCTGATAAACCGCTTTGATGATCGCGTCTTTGTTCACCGCGTAGGTCAGAGCCTGGCGAACTTTCACGTCATCGAGTGGTTTTTTCTGCACGTtata                                                                                                                                <  2:2218703/150‑1 (MQ=255)
                       gtTATAGCCCCACATGGTTGGCGGGATCAGGTTTTTCGCTGATACGCCCGCGCCCTGATAAACCGCTTTGATGATCGCGTCTTTGTTCACCGCGTAGGTCAGAGCCTGGCGAACTTTCACGTCATCGAGTGGTTTTTTCTGCACGTtata                                                                                                                                >  2:338659/1‑150 (MQ=255)
                       gtTATAGCCCCACATGGTTGGCGGGATCAGGTTTTTCGCTGATACGCCCGCGCCCTGATAAACCGCTTTGATGATCGCGTCTTTGTTCACCGCGTAGGTCAGAGCCTGGCGAACTTTCACGTCATCGAGTGGTTTTTTCTGCACGTtata                                                                                                                                >  1:4519859/1‑150 (MQ=255)
                        ttATAGCCCCACATGGTTGGCGGGATCAGGTTTTTCGCTGATACGCCCGCGCCCTGATAAACCGCTTTGATGATCGCGTCTTTGTTCACCGCGTAGGTCAGAGCCTGGCGAACTTTCACGTCATCGAGTGGTTTTTTCTTCACGTTATAc                                                                                                                               >  1:3083139/1‑150 (MQ=255)
                          ataGCCCCACATGGTTGGCGGGATCAGGTTTTTCGCTGATACGCCCGCGCCCTGATAAACCGCTTTGATGATCGCGTCTTTGTTCACCGCGTAGGTCAGAGCCTGGCGAACTTTCACGTCATCGAGTGGTTTTTTCTGCACGTTATACga                                                                                                                             >  2:2629646/1‑150 (MQ=255)
                           taGCCCCACATGGTTGGCGGGATCAGGTTTTTCGCTGATACGCCCGCGCCCTGATAAACCGCTTTGATGATCGCGTCTTTGTTCACCGCGTAGGTCAGAGCCTGGCGAACTTTCACGTCATCTAGTGGTTTTTTCTGCACGTTATACgag                                                                                                                            >  2:3768800/1‑150 (MQ=255)
                           taGCCCCACATGGTTGGCGGGATCAGGTTTTTCGCTGATACGCCCGCGCCCTGATAAACCGCTTTGATGATCGCGTCTTTGTTCACCGCGTAGGTCAGAGCCTGGCGAACTTTCACGTCATCTAGTGGTTTTTTCTGCACGTTATACgag                                                                                                                            >  2:3473867/1‑150 (MQ=255)
                             gCCCCACATGGTTGGCGGGATCAGGTTTTTCGCTGATACGCCCGCGCCCTGATAAACCGCTTTGATGATCGCGTCTTTGTTCACCGCGTAGGTCAGAGCCTGGCGAACTTTCACGTCATCGAGTGGTTTTTTCTGCACGTTATACgagag                                                                                                                          <  1:2645444/150‑1 (MQ=255)
                               cccACATGGTTGGCGGGATCAGGTTTTTCGCTGATACGCCCGCGCCCTGATAAACCGCTTTGATGATCGCGTCTTTGTTCACCGCGTAGGTCAGAGCCTGGCGAACTTTCACGTCATCGAGTGGTTTTTTCTGCACGTTATACGAGAGAt                                                                                                                        <  2:4523284/150‑1 (MQ=255)
                                 cacaTGGTTGGCGGGATCAGGTTTTTCGCTGATACGCCCGCGCCCTGATAAACCGCTTTGATGATCGCGTCTTTGTTCACCGCGTAGGTCAGAGCCTGGCGAACTTTCACGTCATCGAGTGGTTTTTTCTGCACGTTATACGAGAGATaa                                                                                                                      >  1:3360352/1‑150 (MQ=255)
                                  acaTGGTTGGCGGGATCATGTTTTTCGCTGATACGCCCGCGCCCTGATAATCCGATTTGATGATCGCGTCTTTGTTCACCGCGTAGGTCAGAGACTGGCGAACTTTCACGTCATCTAGTTGTTTTTTCTGCACGTTATACGAGAGATAAc                                                                                                                     <  1:4007501/150‑1 (MQ=255)
                                     tGGTTGGCGGGATCAGGTTTTTCGCTGATACGCCCGCGCCCTGATAAACCGCTTTGATGATCGCGTCTTTGTTCACCGCGTAGGTCAGAGCCTGGCGAACTTTCACGTCATCGAGTGGTTTTTTCTGCACGTTATACGAGAGATAACCGa                                                                                                                  <  2:3278620/150‑1 (MQ=255)
                                      ggTTGGCGGGATCAGGTTTTTCGCTGATACGCCCGCGCCCTGATAAACCGCTTTGATGATCGCGTCTTTGTTCACCGCGTAGGTCAGAGCCTGGCGAACTTTCACGTCATCTAGTGGTTTTTTCTGCACGTTATACGAGAGATAACCGAc                                                                                                                 <  2:507704/150‑1 (MQ=255)
                                        ttGGCGGGATCAGGTTTTTCGCTGATACGCCCGCGCCCTGATAAACCGCTTTGATGATCGCGTCTTTGTTCACCGCGTAGGTCAGAGCCTGGCGAACTTTCACGTCATCGAGTGGTTTTTTCTGCACGTTATACGAGAGATAACCGACGt                                                                                                               <  2:577005/150‑1 (MQ=255)
                                         gggCGGGATCAGGTTTTTCGCTGATACGCCCGCGCCCTGATAAACCGCTTTGATGATCGCGTCTTTGTTCACCGCGTAGGTCAGAGCCTGGCGAACTTTCACGTCATCGAGTGGTTTTTTCTGCACGTTATACGAGAGATAACCGACGtt                                                                                                              <  2:3624504/149‑1 (MQ=255)
                                          ggCGGGATCAGGTTTTTCGCTGATACGCCCGCGCCCTGATAAACCGCTTTGATGATCGCGTCTTTGTTCACCGCGTAGGTCAGAGCCTGGCGAACTTTCACGTCATCTAGTGGTTTTTTCTGCACGTTATACGAGAGATAACCGACGTTc                                                                                                             <  2:3479330/150‑1 (MQ=255)
                                           gCGGGATCAGGTTTTTCGCTGATACGCCCGCGCCCTGATAAACCGCTTTGATGATCGCGTCTTTGTTCACCGCGTAGGTCAGAGCCTGGCGAACTTTCACGTCATCGAGTGGTTTTTTCTGCACGTTATACGAGAGATAACCGACGTTCa                                                                                                            <  2:2281624/150‑1 (MQ=255)
                                            cGGGATCAGGTTTTTCGCTGATACGCCCGCGCCCTGATAAACCGCTTTGATGATCGCGTCTTTGTTCACCGCGTAGGTCAGAGCCTGGCGAACTTTCACGTCATCGAGTGGTTTTTTCTGCACGTTATACGAGAGATAACCGACGTTCAg                                                                                                           <  2:4387236/150‑1 (MQ=255)
                                             gggATCAGGTTTTTCGCTGATACGCCCGCGCCCTGATAAACCGCTTTGATGATCGCGTCTTTGTTCACCGCGTAGGTCAGAGCCTGGCGAACTTTCACGTCATCGAGTGGTTTTTTATGCACGTTATACGAGAGATAACCGACGGTCAGc                                                                                                          >  2:1122180/1‑150 (MQ=255)
                                                aTCAGGTTTTTCGCTGATACGCCCGCGCCCTGATAAACCGCTTTGATGATCGCGTCTTTGTTCACCGCGTAGGTCAGAGCCTGGCGAACTTTCACGTCATCTAGTGGTTTTTTCTGCACGTTATACGAGAGATAACCGACGTTCAGcccc                                                                                                       >  2:517252/1‑150 (MQ=255)
                                                   aGGTTTTTCGCTGATACGCCCGCGCCCTGATAAACCGCTTTGATGATCGCGTCTTTGTTCACCGCGTAGGTCAGAGCCTGGCGAACTTTCACGTCATCGAGTGGTTTTTTCTGCCCGTTATACGAGAGAGAACCGCCGGTCAGCCCCCGc                                                                                                    >  1:2781811/1‑150 (MQ=255)
                                                    ggTTTTTCGCTGATACGCCCGCGCCCTGATAAACCGCTTTGATGATCGCGTCTTTGTTCACCGCGTAGGTCAGAGCCTGGCGAACTTTCACGTCATCGAGTGGTTTTTTCTGCACGTTATACGAGAGATAACCGACGTTCAGCCCCGGCa                                                                                                   >  2:2212166/1‑150 (MQ=255)
                                                     gTTTTTCGCTGATACGCCCGCGCCCTGATAAACCGCTTTGATGATCGCGTCTTTGTTCACCGCGTAGGTCAGAGCCTGGCGAACTTTCACGTCATCGAGTGGTTTTTTCTGCACGTTATACGAGTGATAACCGACGTTCAGCCCCGGCAt                                                                                                  >  1:2505045/1‑150 (MQ=255)
                                                     gTTTTTCGCTGATACGCCCGCGCCCTGATAAACCGCTTTGATGATCGCGTCTTTGTTCACCGCGTAGGTCAGAGCCTGGCGAACTTTCACGTCATCGAGTGGTTTTTTCTGCACGTTATACGAGAGATAACCGACGTTCAGCCCCGGCAt                                                                                                  <  1:361303/150‑1 (MQ=255)
                                                     gTTTTGCGCTGATACGCCCGCGCCCGGATAAACCGCTTTGATGATCGCGTCTTTGTTCACCGCGTAGGTCAGAGCCTGGCGAACTTTCACGTCATCGAGTGGTTTTTTCTGCACGTTATACGAGAGATAACCGACGTTCAGCCCCGGCAt                                                                                                  <  2:4009536/150‑1 (MQ=255)
                                                         ttCGCTGATACGCCCGCGCCCTGATAAACCGCTTTGATGATCGCGTCTTTGTTCACCGCGTAGGTCAGAGCCTGGCGAACTTTCACGTCATCGAGTGGTTTTTTCTGCACGTTATACGAGAGATAACCGACGTTCAGCCCCGGCATTTcc                                                                                              <  1:176423/150‑1 (MQ=255)
                                                           cGCTGATACGCCCGCGCCCTGATAAACCGCTTTGATGATCGCGTCTTTGTTCACCGCGTAGGTCAGAGCCTGGCGAACTTTCACGTCATCGAGTGGTTTTTTCTGCACGTTATACGAGAGATAACCGACGTTCAGCCCCGGCATTTccct                                                                                            >  1:2929248/1‑148 (MQ=255)
                                                             cTGATACGCCCGCGCCCTGATAAACCGCTTTGATGATCGCGTCTTTGTTCACCGCGTAGGTCAGAGCCTGGCGAACTTTCACGTCATCGAGTGGTTTTTTCTGCACGTTATACGAGAGATAACCGACGTTCAGCCCCGGCATTTCCATCa                                                                                          <  1:4262703/150‑1 (MQ=255)
                                                             cTGATACGCCCGCGCCCTGATAAACCGCTTTGATGATCGCGTATTTGTTCACCGCGTAGGTCAGAGCCTGGCGAACTTTCACGTCATCGAGTGGTTTTTTCTTCACGTTTTACGTGAGATAACCGACGTTCAGCCCCGGCATTTCCATCa                                                                                          <  1:609491/150‑1 (MQ=255)
                                                                  aCGCCCGCGCCCTGATAAACCGCTTTGATGATCGCGTCTTTGTTCACCGCGTAGGTCAGAGCCTGGCGAACTTTCACGTCATCGAGTGGTTTTTTCTGCACGTTATACGAGAGATAACCGACGTTCAGCCCCGGCATTTCCATCAGATTg                                                                                     <  1:660156/150‑1 (MQ=255)
                                                                    gCCCGCGCCCTGATAAACCGCTTTGATGATCGCGTCTTTGTTCACCGCGTAGGTCAGAGCCTGGCGAACTTTCACGTCATCTAGTGGTTTTTTCTGCACGTTATACGAGAGATAACCGACGTTCAGCCCCGGCATTTCCATCAGATTGAt                                                                                   <  2:1797807/150‑1 (MQ=255)
                                                                    gCCCGCGCCCTGATAAACCGCTTTGATGATCGCGTCTTTGTTCACCGCGTAGGTCAGAGCCTGGCGAACTTTCACGTCATCTAGTGGTTTTCTCTGCACGTTATACGAGAGATAACCGACGTTCAGCCCCGGCATTTCCATCAGATTGAt                                                                                   <  2:1797839/150‑1 (MQ=255)
                                                                        gcgcCCTGATAAACCGCTTTGATGATCGCGTCTTTGTTCACCGCGTAGGTCAGAGCCTGGCGAACTTTCACGTCATCGAGTGGTTTTTTCTGCACGTTATACGAGAGATAACCGACGTTCAGCCCCGGCATTTCCATCAGATTGATGGAt                                                                               <  1:2979982/150‑1 (MQ=255)
                                                                         cgcCCTGATAAACCGCTTTGATGATCGCGTCTTTGTTCACCGCGTAGGTCAGAGCCTGGCGAACTTTCACGTCATCGAGTGGTTTTTTCTGCACGTTATACGAGAGATAACCGACGTTCAGCCCCGGCATTTCCATCAGATTGATGGAtt                                                                              >  2:296505/1‑150 (MQ=255)
                                                                         cgcCCTGATAAACCGCTTTGATGATCGCGTCTTTGGTCACCGCGTAGGTCAGCGCCTGGCGAACTTTCACGTCATCGAGTGGTTTTTTCTGCACGTTATACGAGAGATAACCGACGTTCAGCCCCGGCATTTCCATCAGATTGAGGGAtt                                                                              <  2:2386492/150‑1 (MQ=255)
                                                                            ccTGATAAACCGCTTTGATGATCGCGTCTTTGTTCACCGCGTAGGTCAGAGCCTGGCGAACTTTCACGTCATCGAGTGGTTTTTTCTGCACGTTATACGAGAGATAACCGACGTTCAGCCCCGGCATTTCCATCAGATTGATGGATTTAt                                                                           <  2:3314223/150‑1 (MQ=255)
                                                                            ccTGATAAACCGCTTTGATGATCGCGTCTTTGTTCACCGCGTAGGTCAGAGCCTGGCGAACTTTCACGTCATCGAGTGGTTTTTTCTGCACGTTATACGAGAGATAACCGACGTTCAGCCCCGGCATTTCCATCAGATTGATGGATTTAt                                                                           <  1:3324011/150‑1 (MQ=255)
                                                                             cTGATAAACCGCTTTGATGATCGCGTCTTTGTTCACCGCGTAGGTCAGAGCCTGGCGAACTTTCACGTCATCGAGTGGTTTTTTCTGCACGTTATACGAGAGATAACCGACGTTCAGCCCCGGCATTTCCATCAGAGTGATGGATTTATc                                                                          >  2:337896/1‑150 (MQ=255)
                                                                                aTAAACCGCTTTGATGATCGCGTCTTTGTTCACCGCGTAGGTCAGAGCCTGGCGAACTTTCACGTCATCGAGTGGTTTTTTCTGCACGTTATACGAGAGATAACCGACGTTCAGCCCCGGCATTTCCATCAGATTGATGGATTTATCCTg                                                                       <  2:1088284/150‑1 (MQ=255)
                                                                                aTAAACCGCTTTGATGATCGCGTCTTTGTTCACCGCGTAGGTCAGAGCCTGGCGAACTTTCACGTCATCGAGTGGTTTTTTCTGCACGTTATACGAGAGATAACCGACGTTCAGCCCCGGCATTTCCATCAGATTGATGGATTTATCCTg                                                                       <  1:4314972/150‑1 (MQ=255)
                                                                                  aaaCCGCTTTGATGATCGCGTCTTTGTTCACCGCGTAGGTCAGAGCCTGGCGAACTTTCACGTCATCGAGTGGTTTTTTCTGCACGTTATACGAGAGATAACCGACGTTCAGCCCCGGCATTTCCATCAGATTGATGGATTTATCCTGCt                                                                     <  1:1673005/150‑1 (MQ=255)
                                                                                     ccGCTTTGATGATCGCGTCTTTGTTCACCGCGTAGGTCAGAGCCTGGCGAACTTTCACGTCATCTAGTGGTTTTTTCTGCACGTTATACGAGAGATAACCGACGTTCAGCCCCGGCATTTCCATCAGATTGATGGATTTATCCTGCTTCa                                                                  >  1:2880019/1‑150 (MQ=255)
                                                                                         tttGATGATCGCGTCTTTGTTCACCGCGTAGGTCAGAGCCTGGCGAACTTTCACGTCATCTAGTGGTTTTTTCTGCACGTTATACGAGAGATAACCGACGTTCAGCCCCGGCATTTCCATCAGATTGATGGATTTATCCTGCTTCATgcg                                                              <  2:1777587/150‑1 (MQ=255)
                                                                                            gatgatCGCGTCTTTGTTCACCGCGTAGGTCAGAGCCTGGCGAACTTTCACGTCATCGAGTGGTTTTTTCTGCACGTTATACGAGAGATAACCGACGTTCAGCCCCGGCATTTCCATCAGATTGATTGATTTATCCTGCTTCATgcgagc                                                           >  1:724401/1‑150 (MQ=255)
                                                                                                  cgcgTCTTTGTTCACCGCGTAGGTCAGAGCCTGGCGAACTTTCACGTCATCGAGTGGTTTTTTATTCACCTTATAAGAGAGATAACCGACGTTCAGCCCCGGCATTTACATCAGATTGATGGATTTATCCTGCTTAATTCGAGCGAGATc                                                     >  1:2843563/1‑150 (MQ=255)
                                                                                                   gcgTCTTTGTTCACCGCGTAGGTCAGAGCCTGGCGAACTTTCACGTCATCGAGTGGTTTTTTCTGCACGTTATACGAGAGATAACCGACGTTCAGCCCCGGCATTTCCATCAGATTGATGGATTTATCCTGCTTCATGCGAGCGATATCt                                                    <  1:3921820/150‑1 (MQ=255)
                                                                                                    cgTCTTTGTTCACCGCGTAGGTCAGAGCCTGGCGAACTTTCACGTCATCGAGTGGTTTTTTCTGCACGTTATACGAGAGATAACCGACGTTCAGCCCCGGCATTTCCATCAGATTGATGGATTTATCCTGCTTCATGCGAGCGATATCTg                                                   <  2:891184/150‑1 (MQ=255)
                                                                                                    cgTCTTTGTTCACCGCGTAGGTCAGAGCCTGGCGAACTTTCACGTCATCGAGTGGTTTTTTCTGCACGTTATACGAGAGATAACCGACGTTCAGCCCCGGCATTTCCATCAGATTGATGGATTTATCCTGCTTCATGCGAGCGATATCTg                                                   <  2:4221455/150‑1 (MQ=255)
                                                                                                     gTCTTTGTTCACCGCGTAGGTCAGAGCCTGGCGAACTTTCACGTCATCTAGTGGTTTTTTCTGCACGTTATACGAGAGATAACCGACGTTCAGCCCCGGCATTTCCATCAGATTGATGGATTTATCCTGCTTCATGCGAGCGATATCTGc                                                  <  2:1731347/150‑1 (MQ=255)
                                                                                                     gTCTTTGTTCACCGCGTAGGTCAGAGCCTGGCGAACTTTCACGTCATCGAGTGGTTTTTTCTGCACGTTATACGAGAGATAACCGACGTTCAGCCCCGGCATTTCCATCAGATTGATGGATTTATCCTGCTTCATGCGCGCGATATCTGc                                                  >  2:1225847/1‑150 (MQ=255)
                                                                                                       cTTTGTTCACCGCGTAGGTCAGAGCCTGGCGAACTTTCACGTCATCGAGTGGTTTTTTCTGCACGTTATAAGAGAGATAACCGACGTTAAGCCCCGGCAATTCCATCAGATTGATGGATTTATCCTGCTTCATGCGAGCGATATCTGCCg                                                >  1:1052086/1‑150 (MQ=255)
                                                                                                        tttGTTCACCGCGTAGGTCAGAGCCTGGCGAACTTTCACGTCATCTAGTGGTTTTTTCTGCACGTTATACGAGAGATAACCGACGTTCAGCCCCGGCATTTCCATCAGATTGATGGATTTATCCTGCTTCATGCGAGCGATATCTGCCgg                                               <  2:250145/150‑1 (MQ=255)
                                                                                                         ttGTTCACCGCGTAGGTCAGAGCCTGGCGAACTTTCACGTCATCGAGTGGTTTTTTCTGCACGTTATACGAGAGATAACCGACGTTCAGCCCCGGCATTTCCATCAGATTGATGGATTTATACTGCTTCATGCGAGCGCTATCTGCCggg                                              >  2:4280341/1‑150 (MQ=255)
                                                                                                          tggtCACCGCGTAGGTCAGAGCCTGGCGAACTTTCACGTCATCGAGTGGTTTTTTCTGCACGTTATACGAGAGATAACCGACGTTCAGCCCCGGCATTTCCATCAGATTGATGGATTTATCCTGCTTCATGCGAGCGATATCTGCCGGGt                                             <  2:3009992/147‑1 (MQ=255)
                                                                                                          tGTTCACCGCGTAGGTCAGAGCCTGGCGAACTTTCACGTCATCGAGTGGTTTTTTGTGGACGTTATAAGAGAGATAAACCACGTTCAGCCCCCGCAATTCCATCAGATTGATGGATTTATAATGCTTCATGCGAGCGATATATTCCgggg                                             >  1:438161/1‑149 (MQ=255)
                                                                                                            ttCACCGCGTAGGTCAGAGCCTGGCGAACTTTCACGTCATCTAGTGGTTTTTTCTGCACGTTATACGAGAGATAACCGACGTTCAGCCCCGGCATTTCCATCAGATTGATGGATTTATCCTGCTTCATGCGAGCGATATCTGCCGGGTTc                                           >  2:3242492/1‑150 (MQ=255)
                                                                                                               aCCGCGTAGGTCAGAGCCTGGCGAACTTTCACGTCATCGAGTGGTTTTTTCTGCACGTTATACGAGAGATAACCGACGTTCAGCCCCGGCATTTCCATCAGATTGATGGATTTATCCTGCTTCATGCGAGCGATATCTGCCGGGTTCggg                                        >  2:2553521/1‑150 (MQ=255)
                                                                                                                ccGCGTAGGTCAGAGCCTGGCGAACTTTCACGTCATCGAGTGGTTTTTTCTGCACGTTATACGAGAGATAACCGACGTTCAGCCCCGGCATTTCCATCAGATTGATGGATTTATCCTGCTTCATGCGAGCGATATCTGCCGGGTTCGGGt                                       <  1:4187362/150‑1 (MQ=255)
                                                                                                                         tCAGAGCCTGGCGAACTTTCACGTCATCGAGTGGTTTTTTCTGGACGTTATAAGAGAGATAAACGACGTTAAGCCCCCGCATTTCCAAAAGATTTATGGATTTTTACTGCTTCAAGAGAGCGATATCTGCCGGGGTCGGGTACGGCaaaa                              >  1:2033003/1‑147 (MQ=255)
                                                                                                                         tCAGAGCCTGGCGAACTTTCACGTCATCGAGTGGTTTTTTCTGCACGTTATACGAGAGATAACCGACGTTCAGCCCCGGCATTTCCATCAGATTGATGGATTTATCCTGCTTCATGCGAGCGATATCTGCCGGGTTCGGGTACGGCATCa                              <  1:2536485/150‑1 (MQ=255)
                                                                                                                         tCAGAGCCTGGCGAACTTTCACGTCATCGAGTGGTTTTTTATTCACGTTATAAGAGAGATAACCCACGTTAAGCCCCGGCAGTTGCATAAGATTGATTGATTTATACTGCTTCATGCGAGCGATATCTTCCGGGTTCGGGTACGGCATCa                              >  1:1633755/1‑150 (MQ=255)
                                                                                                                           agagCCTGGCGAACTTTCACGTCATCGAGTGGTTTTTTATGCAACTTATAAGAGAGATAACCGCCGGTAAGCCCCCGCAATTACAACCCAATGATTGATTTATACTGCTTAAAGAGAGCGATATATGCCGGGTTCGGGTACCGCATCAcc                            >  1:1242187/1‑150 (MQ=255)
                                                                                                                            gagCCTGGCGAACTTTCACGTCATCGAGTGGTTTTTTCTGCACGTTATACGAGAGATAACCGACGTTCAGCCCCGGCATTACCATCAGATTGATGGATTTATCCTGCTTCATGCGAGCGATATCTGCCGGGTTCGGGTACGGCATCACCt                           <  1:248335/150‑1 (MQ=255)
                                                                                                                             agCCTGGCGAACTTTCACGTCATCTAGTGGTTTTTTCTGCACGTTATACGAGAGATAACCGACGTTCAGCCCCGGCATTTCCATCAGGTTGATGGATTTATCCTGCTTCATGCGAGCGATATCTGCCGGGTTCGGGTACGGCATCACCTg                          <  1:3877175/150‑1 (MQ=255)
                                                                                                                             agCCTGGCGAACTTTCACGTCATCGAGTGGTTTTTTCTGCACGTTATACGAGAGATAACCGACGTTCAGCCCCGGCATTTCCATCAGATTGATGGATTTATCCTGCTTCATGCGAGCGATATCTGCCGGGTTCGGGTACGGCATCACCTg                          <  2:131239/150‑1 (MQ=255)
                                                                                                                              gCCTGGCGAACTTTCACGTCATCGAGTGGTTTTTTCTGCACGTTATACGAGAGATAACCGACGTTCAGCCCCGGCATTTCCATCAGATTGATGGATTTATCCTGCTTCATGCGAGCGATATCTGCCGGGTTCGGGTACGGCATCACCTgg                         <  2:285536/150‑1 (MQ=255)
                                                                                                                                cTGGCGAACTTTCACGTCATCTAGTGGTTTTTTCTGCACGTTATACGAGAGATAACCGACGTTCAGCCCCGGCATTTCCATCAGATTGATGGATTTATCCTGCTTCATGCGAGCGATATCTGCCGGGTTCGGGTACGGCATCACCTGGca                       <  1:3525379/150‑1 (MQ=255)
                                                                                                                                cTGGCGAACTTTCACGTCATCGAGTGGTTTTTTCTGCACGTTATACGAGAGATAACCGACGTTCAGCCCCGGCATTTACATCAGATTTATGGATTTATCCTGCTTCATGCGAGCGATATCTGCCGGGTTCGGGTACGGCATCACCTGGcc                       >  1:2167915/1‑149 (MQ=255)
                                                                                                                                 tGGCGAACTTTCACGTCATCGAGTGGTTTTTTATGCACGTTATAAGAGAGATAACCGACGTTCAGCCCCCGCATTTACATAAGATTGATGGATTTATCCTGCTTAATTCGAGCGATATCTGCCGGGTTCGGGTACGGCATCACCAGGcat                      >  2:3424851/1‑150 (MQ=255)
                                                                                                                                  ggCGAACTTTCACGTCATCGAGTGGTTTTTTCTGCACGTTATACGAGAGATAACCGACGTTCAGCCCCGGCATTTCCATCAGATTGATGGATTTATCCTGCTTCATGCGAGCGATATCTGCCGGGTTCGGGTACGGCATCACCTGGcatt                     <  2:1514342/150‑1 (MQ=255)
                                                                                                                                    cGAACTTTCACGTCATCGAGTGGTTTTTTCTGCACGTTATAAGAGAGATAAACGACGTTCAGCCCCGGCATTTCCATCAGATTGATGGATTTATACTGCTTCATGCGAGCGATATCTGCCGGGTTCGGGTACGGCATCACCAGGcattca                   >  1:313557/1‑150 (MQ=255)
                                                                                                                                      aaCTTTCACGTCATCGAGTGGTTTTTTCTGCACGTTATACGAGAGCTAACCGACGTTCAGCCCCGGCATATCAATCAGATGGATGGATTTAAACTGCTTCATGATAGCCAAAACTGCCGGGTTCGGGTACGGCATCACCTGGcattcatt                 >  2:1399249/1‑150 (MQ=255)
                                                                                                                                         tttCACGTCATCGAGTGGTTTTTTCTGCACGTTATACGAGAGATAACCGACGTTCAGCCCCGGCATTTCCATCAGATTGATGGATTTATCCTGCTTCATGCGAGCGATATCTGCCGGGTTCGGGTACGGCATCACCTGGCATTCAttctt              <  2:4288368/150‑1 (MQ=255)
                                                                                                                                         tttCACGTCATCGAGTGGTTTTTTCTGCACGTTATACGAGAGATAACCGACGTTCAGCCCCGGCATTTCCATCAGATTGATGGATTTATCCTGCTTCATGCGAGCGATATCTGCCGGGTTCGGGTACGGCATCACCTGGCATTCAttctt              <  1:3438649/150‑1 (MQ=255)
                                                                                                                                          ttCACGTCATCGTGTGGTTTTTTCTGCACGTTATACGAGAGATAACCGACGTTCAGCCCCGGCATTTCCATCAGATTGATGGATTTATCCTGCTTCATGCGAGCGATATCTGCCGGGTTCGGGTACGGCATCACCTGGCATTCAttcttc             <  1:1122180/150‑1 (MQ=255)
                                                                                                                                           tCACGTCATCGAGTGGTTTTTTCTGCACGTTATACGAGAGATAACCGACGTTCAGCCCCGGCATTTCCATCAGATTGATGGATTTATCCTGCTTCATGCGAGCGATATCTGCCGGGTTCGGGTACGGCATCACCAGGCATTCATtcttct            >  2:1873602/1‑150 (MQ=255)
                                                                                                                                            cACGTCATCTAGTGGTTTTTTCTGCACGTTATACGAGAGATAACCGACGTTCAGCCCCGGCATTTCCATCAGATTGATGGATTTATCCTGCTTCATGCGAGCGCTATCTGCCGGGTTCGGGTACGGCATCACCTGGCATTCATTCTTCTg           >  2:276377/1‑150 (MQ=255)
                                                                                                                                              cGTCATCGAGTGGTTTTTTCTGCACGTTATACGAGAGATAACCGACGTTCAGCCCCGGCATTTCCATCAGATTGATGGATTTATCCTGCTTCATGCGAGCGATATCTGCCGGGTTCGGGTACGGCATCACCTGGCATTCATTCTTCTGCa         >  2:361612/1‑150 (MQ=255)
                                                                                                                                               gTCATCGAGTGGTTTTTTCTGCACGTTATACGAGAGATAACCGACGTTCAGCCCCGGCATTTCCAACAGATTGATGGATTTAACCTGCTTAAAGCGAGCGATATCTGCCGGGTTCGGGTACGGCATCACCTGGCATTCATTCTTCTGCaa        >  2:2490970/1‑150 (MQ=255)
                                                                                                                                                tCATCGAGTGGTTTTTTCTGCACGTTATACGAGAGATAACCGACGTTCAGCCCCGGCATTTCCATCAGATTGATGGATTTATCCTGCTTCATGCGAGCGATATCTGCCGGGTTCGGGTACGGCATCACCTGGCATTCATTCTTCTGCAAt       <  1:2401138/150‑1 (MQ=255)
                                                                                                                                                     gAGTGGTTTTTTCTGCACGTTATACGAGAGATAACCGACGTTCAGCCCCGGCATTTCCATCAGATTGATGGATTTATCCTGCTTCATGCGAGCGATATCTGCCGGGTTCGGGTACGGCATCACCTGGCATTCATTCTTCTGCAATTTcgc  <  1:2417373/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
TAGGTGTAGTCCTGAACGTCGTCGTTATAGCCCCACATGGTTGGCGGGATCAGGTTTTTCGCTGATACGCCCGCGCCCTGATAAACCGCTTTGATGATCGCGTCTTTGTTCACCGCGTAGGTCAGAGCCTGGCGAACTTTCACGTCATCGAGTGGTTTTTTCTGCACGTTATACGAGAGATAACCGACGTTCAGCCCCGGCATTTCCATCAGATTGATGGATTTATCCTGCTTCATGCGAGCGATATCTGCCGGGTTCGGGTACGGCATCACCTGGCATTCATTCTTCTGCAATTTCGC  >  CP009273/3700005‑3700303

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑