Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,774,382 T→G 12.7% intergenic (+5/‑193) lldD → / → trmL L‑lactate dehydrogenase, FMN‑linked/tRNA Leu mC34,mU34 2'‑O‑methyltransferase, SAM‑dependent

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,774,3820TG12.7% 346.0 / 13.2 142intergenic (+5/‑193)lldD/trmLL‑lactate dehydrogenase, FMN‑linked/tRNA Leu mC34,mU34 2'‑O‑methyltransferase, SAM‑dependent
Reads supporting (aligned to +/- strand):  ref base T (11/109);  new base G (19/3);  total (30/112)
Fisher's exact test for biased strand distribution p-value = 3.53e-13
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 145 total aligned reads displayed.

CTGATCGAAAAAGAGATGAAAGTGGCGATGACGCTGACTGGCGCGAAATCGATCAGCGAAATTACGCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGC‑G‑CCCCCTC‑ACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCAC  >  CP009273/3774234‑3774528
                                                                                                                                                    |                                                                                                                                                     
cTGATCGAAAAAGAGATGAAAGTGGCGATGACGCTGACTGGCGCGAAATCGATCAGCGAAATTACGCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGttt                                                                                                                                                      >  2:2637370/1‑150 (MQ=255)
  gATCGAAAAAGAGATGAAAGTGGCGATGACGCTGACTGGCGCGAAATCGATCAGCGAAATTACGCAAGAGTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTgc                                                                                                                                                    <  2:248065/150‑1 (MQ=255)
        aaaaGAGATGAAAGTGGCGATGACGCTGACTGGCGCGAAATCGATCAGCGAAATTACGCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGC‑c‑ccccc                                                                                                                                            >  2:1851215/1‑150 (MQ=255)
        aaaaGAGATGAAAGTGGCGATGACGCTGACTGGCGCGAAATCGATCAGCGAAATTACGCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTGTGC‑G‑ccccc                                                                                                                                            >  2:220424/1‑150 (MQ=255)
         aaaGAGATGAAAGTGGCGATGACGCTGACTGGCGCGAAATCGATCAGCGAAATTACGCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGC‑G‑CCCCCt                                                                                                                                           <  1:4224147/150‑1 (MQ=255)
         aaaGAGATGAAAGTGGCGATGACGCTGACTGGCGCGAAATCGATCAGCGAAATTACGCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGC‑G‑CCCCCt                                                                                                                                           <  1:4223963/150‑1 (MQ=255)
          aaGAGATGAAAGTGGCGATGACGCTGACTGGCGCGAAATCGATCAGCGAAATTACGCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTTC‑G‑ccccccc                                                                                                                                          >  1:1677729/1‑148 (MQ=255)
           agagATGAAAGTGGCGATGACGCTGACTGGCGCGAAATCGATCAGCGAAATTACGCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGC‑G‑CCCCCTC‑a                                                                                                                                        <  1:28121/150‑1 (MQ=255)
           agagATGAAAGTGGCGATGACGCTGACTGGCGCGAAATCGATCAGCGAAATTACGCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTGTGG‑G‑CCCCCCC‑a                                                                                                                                        >  1:4148091/1‑150 (MQ=255)
           agagATGAAAGTGGCGATGACGCTGACTGGCGCGAAATCGATCAGCGAAATTACGCAAGAATCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGC‑G‑CCCCCCC‑a                                                                                                                                        >  1:3347842/1‑150 (MQ=255)
             agaTGAAAGTGGCGATGACGCTGACTGGCGCGAAATCGATCAGCGAAATTACGCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGC‑G‑CCCCCTC‑Acc                                                                                                                                      >  2:4336808/1‑150 (MQ=255)
              gaTGAAAGTGGCGATGACGCTGACTGGCGCGAAATCGATCAGCGAAATTACGCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGC‑G‑CCCCCTC‑Accc                                                                                                                                     <  2:2940687/150‑1 (MQ=255)
                tGAAAGTGGCGATGACGCTGACTGGCGCGAAATCGATCAGCGAAATTACGCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGC‑G‑CCCCCTC‑ACCCTa                                                                                                                                   <  1:4495059/150‑1 (MQ=255)
                tGAAAGTGGCGATGACGCTGACTGGCGCGAAATCGATCAGCGAAATTACGCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGC‑G‑CCCCCTC‑ACCCTa                                                                                                                                   <  1:4336808/150‑1 (MQ=255)
                   aaGTGGCGATGACGCTGACTGGCGCGAAATCGATCAGCGAAATTACGCAAGCTTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGC‑G‑CCCCCTC‑ACCCTAAcc                                                                                                                                <  1:825176/150‑1 (MQ=255)
                   aaGTGGCGATGACGCTGACTGGCGCGAAATCGATCAGCGAAATTACGCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGC‑G‑CCCCCTC‑ACCCTAAcc                                                                                                                                <  2:1919340/150‑1 (MQ=255)
                   aaGTGGCGATGACGCTGACTGGCGCGAAATCGATCAGCGAAATTACGCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGC‑G‑CCCCCTC‑ACCCTAAcc                                                                                                                                <  2:3490502/150‑1 (MQ=255)
                   aaGTGGCGATGACGCTGACTGGCGCGAAATCGATCAGCGAAATTACGCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGC‑G‑CCCCCTC‑ACCCTAAcc                                                                                                                                <  2:1204795/150‑1 (MQ=255)
                    aGTGGCGATGACGCTGACTGGCGCGAAATCGATCAGCGAAATTACGCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGC‑G‑CCCCCTC‑ACCCTAAccc                                                                                                                               <  2:273618/150‑1 (MQ=255)
                    aGTGGCGATGACGCTGACTGGCGCGAAATCGATCAGCGAAATTACGCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGC‑G‑CCCCCTC‑ACCCTAAccc                                                                                                                               <  2:273675/150‑1 (MQ=255)
                    aGTGGCGATGACGCTGACTGGCGCGAAATCGATCAGCGAAATTACGCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTGTGG‑G‑Gcccccc‑cccccacaac                                                                                                                               >  1:1619656/1‑144 (MQ=255)
                     gTGGCGATGACGCTGACTGGCGCGAAATCGATCAGCGAAATTACGCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTGTGG‑G‑CCCCCCCAACCCAAAccc                                                                                                                               >  2:1924605/1‑150 (MQ=255)
                     gTGGCGATGACGCTGACTGGCGCGAAATCGATCAGCGAAATTACGCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTGTGC‑G‑CCCCCCC‑CACCAAAccca                                                                                                                              >  2:1924608/1‑149 (MQ=255)
                        gCGATGACGCTGACTGGCGCGAAATCGATCAGCGAAATTACGCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGC‑G‑CCCCCTC‑ACCCTAACCCtctc                                                                                                                           <  1:2797214/150‑1 (MQ=255)
                        gCGATGACGCTGACTGGCGCGAAATCGATCAGCGAAATTACGCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGC‑G‑CCCCCTC‑ACCCTAACCCtctc                                                                                                                           <  1:3677078/150‑1 (MQ=255)
                         cGATGACGCTGACTGGCGCGAAATCGATCAGCGAAATTACGCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGC‑G‑CCCCCTC‑ACCCTAACCCTCTcc                                                                                                                          <  1:162454/150‑1 (MQ=255)
                         cGATGACGCTGACTGGCGCGAAATCGATCAGCGAAATTACGCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGC‑G‑CCCCCAC‑ACCCGAAaccacgcc                                                                                                                          >  1:706332/1‑145 (MQ=255)
                         cGATGACGCTGACTGGCGCGAAATCGATCAGCGAAATTACGCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTGTGG‑G‑GCCCCCC‑CCCCCCAcccgcacc                                                                                                                          >  1:2725960/1‑145 (MQ=255)
                          gATGACGCTGACTGGCGCGAAATCGATCAGCGAAATTACGCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTGTGG‑G‑CCCCCAC‑CCCCAAACCCCCAccc                                                                                                                         >  2:2198742/1‑150 (MQ=255)
                           aTGACGCTGACTGGCGCGAAATCGATCAGCGAAATTACGCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGC‑G‑CCCCCTC‑ACCCTAACCCTCTCCCt                                                                                                                        <  2:1113192/150‑1 (MQ=255)
                           aTGACGCTGACTGGCGCGAAATCGATCAGCGAAATTACGCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTGTGCGG‑CCCCCCC‑CCCCAAACcccccccc                                                                                                                         >  2:1533170/1‑150 (MQ=255)
                           aTGACGCTGACTGGCGCGAAATCGATCAGCGAAATTACGCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTGTGC‑G‑CCCCCCC‑ACCCAAAACCCCAcccc                                                                                                                        >  2:4147156/1‑149 (MQ=255)
                                gCTGACTGGCGCGAAATCGATCAGCGAAATTACGCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGC‑G‑CCCCCTC‑ACCCTAACCCTCTCCCTCAggg                                                                                                                   <  2:509164/150‑1 (MQ=255)
                                   gACTGGCGCGAAATCGATCAGCGAAATTACGCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGC‑G‑CCCCCTC‑ACCCTAACCCTCTCCCTCAGGgaga                                                                                                                <  2:3459455/150‑1 (MQ=255)
                                     cTGGCGCGAAATCGATCAGCGAAATTACGCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGC‑G‑CCCCCTC‑ACCCTAACCCTCTCCCTCAGGGAGAgg                                                                                                              <  2:228921/150‑1 (MQ=255)
                                     cTGGCGCGAAATCGATCAGCGAAATTACGCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGC‑G‑CCCCCTC‑ACCCTAACCCTCTCCCTCAGGGAGAgg                                                                                                              <  2:3275493/150‑1 (MQ=255)
                                      tGGCGCGAAATCGATCAGCGAAATTACGCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGC‑G‑CCCCCTC‑ACCCTAACCCTCTCCCTCAGGGAGAggg                                                                                                             <  2:357738/150‑1 (MQ=255)
                                       ggCGCGAAATCGATCAGCGAAATTACGCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGC‑G‑CCCCCTC‑ACCCTAACCCTCTCCCTCAGGGAGAgggg                                                                                                            <  2:2327237/150‑1 (MQ=255)
                                       ggCGCGAAATCGATCAGCGAAATTACGCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGC‑G‑CCCCCTC‑ACCCTAACCCTCTCCCTCAGGGAGAgggg                                                                                                            <  2:2328167/150‑1 (MQ=255)
                                       ggCGCGAAATCGATCAGCGAAATTACGCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGC‑G‑CCCCCTC‑ACCCTAACCCTCTCCCTCAGGGAGAgggg                                                                                                            <  2:4137442/150‑1 (MQ=255)
                                        gcgcGAAATCGATCAGCGAAATTACGCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGC‑G‑CCCCCTC‑ACCCTAACCCTCTCCCTCAGGGAGAGGGGa                                                                                                           <  2:4281336/150‑1 (MQ=255)
                                        gcgcGAAATCGATCAGCGAAATTACGCAAGAGTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGC‑G‑CCCCCTC‑ACCCTAACCCTCTCCCTCAGGGAGAGGGGa                                                                                                           <  2:4281314/150‑1 (MQ=255)
                                         cgcgAAATCGATCAGCGAAATTACGCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGC‑G‑CCCCCTC‑ACCCTAACCCTCTCCCTCAGGGAGAGGGGAc                                                                                                          <  2:424626/150‑1 (MQ=255)
                                         cgcgAAATCGATCAGCGAAATTACGCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGC‑G‑CCCCCTC‑ACCCTAACCCTCTCCCTCAGGGAGAGGGGAc                                                                                                          <  2:424627/150‑1 (MQ=255)
                                          gcgAAATCGATCAGCGAAATTACGCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGC‑G‑CCCCCTC‑ACCCTAACCCTCTCCCTCAGGGAGAGGGGAcc                                                                                                         <  1:1344687/150‑1 (MQ=255)
                                            gAAATCGATCAGCGAAATTACGCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTGTTC‑G‑CCCCCCC‑ACCCAAACCCCCTCCCACAGGGGGCCGGGCCCGt                                                                                                       >  2:2715655/1‑150 (MQ=255)
                                             aaaTCGATCAGCGAAATTACGCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGC‑G‑CCCCCTC‑ACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGtt                                                                                                      <  1:1332419/150‑1 (MQ=255)
                                               aTCGATCAGCGAAATTACGCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGC‑G‑CCCCCTC‑ACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCg                                                                                                    <  1:2954993/150‑1 (MQ=255)
                                                  gATCAGCGAAATTACGCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGCATGCGGCATAGTCGTGTGC‑GCCCCCCCC‑CCCCAAACCCCCCCCCCCCGGGGGGGGGGCCCggagggg                                                                                                  >  1:4450574/1‑144 (MQ=255)
                                                  gATCAGCGAAATTACGCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTGTGC‑G‑CCCCCCC‑ACCCAAAACCACACCCACCCGGTGAGGGGGCCCGTCgggg                                                                                                 >  2:1833360/1‑148 (MQ=255)
                                                           aaTTACGCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGC‑G‑CCCCCTC‑ACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCtgtatgta                                                                                        <  2:4540510/150‑1 (MQ=255)
                                                              tACGCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGC‑G‑CCCCCTA‑AACCAAACCCACACCCACACGGAGAGGGGACCCTTAGGGGCggtatttaata                                                                                     >  2:1065401/1‑147 (MQ=255)
                                                               aCGCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGC‑G‑CCCCCTC‑ACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTcc                                                                                    <  1:1317863/150‑1 (MQ=255)
                                                               aCGCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGC‑G‑CCCCCTC‑ACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTcc                                                                                    <  1:1238254/150‑1 (MQ=255)
                                                                cGCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGC‑G‑CCCCCTC‑ACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTccc                                                                                   <  2:3246197/150‑1 (MQ=255)
                                                                 gCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGC‑G‑CCCCCTC‑ACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCt                                                                                  <  1:3650132/150‑1 (MQ=255)
                                                                      aTTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGC‑G‑CCCCCTC‑ACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCActc                                                                             <  1:3369662/150‑1 (MQ=255)
                                                                      aTTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGC‑G‑CCCCCTC‑ACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCActc                                                                             <  2:1014387/150‑1 (MQ=255)
                                                                         cGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGC‑G‑CCCCCTC‑ACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGa                                                                          <  2:434067/150‑1 (MQ=255)
                                                                          gCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGC‑G‑CCCCCTC‑ACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGaa                                                                         <  1:1225043/150‑1 (MQ=255)
                                                                          gCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGC‑G‑CCCCCTC‑ACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGaa                                                                         <  1:1225011/150‑1 (MQ=255)
                                                                          gCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTGTGC‑G‑CCCCCCC‑ACCCAAAACCACACCCACAAGGCGAAGGGACCGCTACGCCCGGGAAGTACTCCCACACACGGaa                                                                         >  2:3395126/1‑150 (MQ=255)
                                                                            tGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTGTGC‑G‑CCCCCGC‑ACCCGCAACCCCTACCCCCGGGGGCGGGGACCGTGCGGCGGGGTATGTGATCCCGCACACTGAAAc                                                                       >  2:4447620/1‑150 (MQ=255)
                                                                                 cAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATTGCGAAAGGGAATGCGGCTTAGTCGTTTGC‑G‑CCCCCTC‑ACCCTAACCCTCTCCCTCAGGGTGAGGGGACCGTTCGGCGCTGTATTTACTCCCTCACTCTGAAACGacac                                                                  <  2:2648371/150‑1 (MQ=255)
                                                                                 cAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGC‑G‑CCCCCTC‑ACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGacac                                                                  <  2:3826863/150‑1 (MQ=255)
                                                                                  tggggCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAATGGGAATGCGGCATAGTCGTTTGC‑G‑CCCCCTC‑ACCCTAACCCTCTCCCACAGGGAGAGGGTACCGTTCGGCGCTGTTTGTACTCCCTCACTCGGAAACGACAcc                                                                 <  1:2536271/149‑1 (MQ=255)
                                                                                  aGGGGCTGGTTAAAGAGTTGCCTGCGGCACTGGCTCCCTTGGCGAATGGGATTGCGGAATTGTCGTTTGC‑G‑CCCCCTC‑ACCCTAACCCTCTCCCTCAGGGTGTGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACAcc                                                                 <  1:2535208/150‑1 (MQ=255)
                                                                                   ggggCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCTTAGTCGTTTGC‑G‑CCCCCTC‑ACCCTAACCCTCTCCCTCAGTGAGATGGTACCGTTCGGCGCTGTATGTACTACCTCCCTCTGAAACGACACCg                                                                <  2:857710/150‑1 (MQ=255)
                                                                                       cTGGGTAAAGAGGTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGC‑G‑CCCCCTC‑ACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCAct                                                            <  2:2875494/150‑1 (MQ=255)
                                                                                         gggTAATGTGTTGCCTGCGGCACTGGCTCCCTTGGCGAATGGGAATGCGGCATAGTCGTTTGC‑G‑CCCCCTC‑ACCCTAACCCTCTCCCTCAGGGAGTGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCActct                                                          <  1:93565/150‑1 (MQ=255)
                                                                                         gggTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGC‑G‑CCCCCTC‑ACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTTTATGTACTCCCTCACTCTGAAACGACACCGCActct                                                          <  1:4364665/150‑1 (MQ=255)
                                                                                           gTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGC‑G‑CCCCCTC‑ACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCttt                                                        <  2:4069735/150‑1 (MQ=255)
                                                                                            tAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGC‑G‑CCCCCTC‑ACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCtttt                                                       <  1:3521190/150‑1 (MQ=255)
                                                                                             aaaGAGTTGCCTGCGGCACTGGCTCCCAGGGCGAAAGGGAATGCGGCATAGTCGTTTGC‑G‑CCCCCTC‑ACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCttttt                                                      <  2:2315525/150‑1 (MQ=255)
                                                                                                gagTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGC‑G‑CCCCCTC‑ACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTtc                                                   <  2:3089734/150‑1 (MQ=255)
                                                                                                gagTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGC‑G‑CCCCCTC‑ACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTtc                                                   <  2:3089733/150‑1 (MQ=255)
                                                                                                   ttGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGC‑G‑CCCCCTC‑ACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTcc                                                <  1:3972759/150‑1 (MQ=255)
                                                                                                    tGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGC‑G‑CCCCCTC‑ACCCTACCCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTccc                                               <  2:91143/150‑1 (MQ=255)
                                                                                                      ccTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGC‑G‑CCCCCCC‑ACCCCAAACCCCTACCACCGGGGGAGGGGGCCGTTGGGGGGGGGTTGTACTCCCACACAAAGTAACCACACCCCACACGTTTTTTATcccac                                             >  2:3000250/1‑148 (MQ=255)
                                                                                                           ggCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGC‑G‑CCCCCTC‑ACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGcccc                                        <  2:2134695/150‑1 (MQ=255)
                                                                                                            gCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGC‑G‑CCCCCTC‑ACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCt                                       <  2:3556825/150‑1 (MQ=255)
                                                                                                             cACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGC‑G‑CCCCCTC‑ACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTc                                      <  2:2924992/150‑1 (MQ=255)
                                                                                                             cACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGC‑G‑CCCCCTC‑ACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTc                                      <  2:2925273/150‑1 (MQ=255)
                                                                                                              aCTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGC‑G‑CCCCCTC‑ACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTcc                                     <  1:2254605/150‑1 (MQ=255)
                                                                                                                      ccATGGCGACAGGGAAGGCGGCATAGCCGTTTGC‑G‑CCCCCTC‑ACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGagag                             <  1:3715952/150‑1 (MQ=255)
                                                                                                                       cATGGCGAAAGGGAATGCGGCATAGTCGTTTGC‑G‑CCCCCTC‑ACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAgg                            <  1:662323/150‑1 (MQ=255)
                                                                                                                              aacgggAATGCGGCAGAGTCGTTGGC‑G‑CCCCCTC‑CCCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCgggg                     <  1:868988/147‑1 (MQ=255)
                                                                                                                              aaaGGGAATGCGGCATAGTCGTTTGC‑G‑CCCCCTC‑ACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCgggg                     <  2:3347842/150‑1 (MQ=255)
                                                                                                                                aGGGAATGCGGCATAGTCGTTTGC‑G‑CCCCCTC‑ACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTg                   <  1:4452308/150‑1 (MQ=255)
                                                                                                                                   gAATGCGGCATAGTCGTTTGC‑G‑CCCCCTC‑ACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAgg                <  2:2137355/150‑1 (MQ=255)
                                                                                                                                   gAATGCGGCATAGTCGTTTGC‑G‑CCCCCTC‑ACCCTAACCCACTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAgg                <  2:401003/150‑1 (MQ=255)
                                                                                                                                    aaTGCGGCATAGTCGTTTGC‑G‑CCCCCTC‑ACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAggg               <  2:1716405/150‑1 (MQ=255)
                                                                                                                                     aTGCGGCATAGTCGTGTGC‑G‑CCCCCTC‑ACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAgggg              <  2:1244073/150‑1 (MQ=255)
                                                                                                                                     aTGCGGCATAGTCGTGGGC‑G‑CCCCCTC‑ACCCTACCCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAgggg              <  2:3528660/150‑1 (MQ=255)
                                                                                                                                       gCGGCATTGGGGTTTGC‑G‑CCCCCTC‑ACCCTACCCCTCGCCCTCCGGGAGAGGGGACCGTGCGGCGCTTTTTGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGTAGAGGGCGGGGGTGAGGGGaa            <  2:2306809/150‑1 (MQ=255)
                                                                                                                                         ggCATCGTCGTGTGC‑G‑CCCCCTC‑ACCCTAACCCTCTCCCTCCGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGaaaa          <  2:130665/150‑1 (MQ=255)
                                                                                                                                          gCATAGTCGTTTGC‑G‑CCCCCTC‑ACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTTTGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAg         <  1:3644101/150‑1 (MQ=255)
                                                                                                                                          gCATAGTCGTTTGC‑G‑CCCCCTC‑ACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAg         <  1:3643692/150‑1 (MQ=255)
                                                                                                                                          gCATAGTCGTTTGC‑G‑CCCCCTC‑ACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAg         <  1:4125553/150‑1 (MQ=255)
                                                                                                                                                 cGTTTGC‑G‑CCCCCGC‑ACCCTAACCCACACCCACAGGGAGAGGGGACCGTTCGGCGCGGTATGTACTCCCTCACACAGAAAAGAAACCGCACACTTTTTTTCTCCCACGCCCCTCCGGGGAGAGGGCCGGGGGGAGGGGAAAAGGCCGCAc  >  2:4096058/1‑150 (MQ=255)
                                                                                                                                                    |                                                                                                                                                     
CTGATCGAAAAAGAGATGAAAGTGGCGATGACGCTGACTGGCGCGAAATCGATCAGCGAAATTACGCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGC‑G‑CCCCCTC‑ACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCAC  >  CP009273/3774234‑3774528

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑