Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,802,238 A→T 20.3% A113A (GCA→GCT)  waaA → 3‑deoxy‑D‑manno‑octulosonic‑acid transferase (KDO transferase)

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,802,2380AT20.3% 679.8 / 171.2 271A113A (GCA→GCT) waaA3‑deoxy‑D‑manno‑octulosonic‑acid transferase (KDO transferase)
Reads supporting (aligned to +/- strand):  ref base A (113/103);  new base T (28/27);  total (141/130)
Fisher's exact test for biased strand distribution p-value = 8.81e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.33e-01

Only 100 of 277 total aligned reads displayed.

GCAATCCCGTTGGTGCGCGCGCTGCGTCATCGTTATCCTGATTTACCGATTACCGTAACAACCATGACGCCAACCGGTTCGGAGCGCGTACAATCGGCTTTCGGGAAGGATGTTCAGCACGTTTATCTGCCGTATGATCTGCCCGATGCACTCAACCGTTTCCTGAATAAAGTCGACCCTAAACTGGTGTTGATTATGGAAACCGAACTATGGCCTAACCTGATTGCGGCGCTACATAAACGTAAAATTCCGCTGGTGATCGCTAACGCGCGACTCTCTGCCCGCTCGGCCGCAGGTTA  >  CP009273/3802089‑3802387
                                                                                                                                                     |                                                                                                                                                     
gcAATCCCGGTGGTGCGCGCGCTGCGTCATCGTTATCCTGATTTACCGATTACCGTAACAACCATGACGCCAACCGGTTCGGAGCGCGTACAATCGGCTTTCGGGAAGGATGTTCAGCACGTTTATCTGCCGTATGATCTGCCCGATGCa                                                                                                                                                       <  2:2857660/150‑1 (MQ=255)
  aaTCCCGTTGGTGCGCGCGCTGCGTCATCGTTATCCTGATTTACCGATTACCGTAACAACCATGACGCCAACCGGTTCGGAGCGCGTACAATCGGCTTTCGGGAAGGATGTTCAGCACGTTTATCTGCCGTATGATCTGCCCGATGCACt                                                                                                                                                     <  1:888527/150‑1 (MQ=255)
   aTCCCGTTGGTGCGCGCGCTGCGTCATCGTTATCCTGATTTACCGATTACCGTAACAACCATGACGCCAACCGGTTCGGAGCGCGTACAATCGGCTTTCGGGAAGGATGTTCAGCACGTTTATCTGCCGTATGATCTGCCCGATGCACTc                                                                                                                                                    >  1:2946405/1‑150 (MQ=255)
       cGTTGGTGCGCGCGCTGCGTCATCGTTATCCTGATTTACCGATTACCGTAACAACCATGACGCCAACCGGTTCGGAGCGCGTACAATCGGCTTTCGGGAAGGATGTTCAGCACGTTTATCTGCCGTATGATCTGCCCGATGCACTCAAcc                                                                                                                                                <  2:838009/150‑1 (MQ=255)
             tGCGCGCGCTGCGTCATCGTTATCCTGATTTACCGATTACCGTAACAACCATGACGCCAACCGGTTCGGAGCGCGTACAATCGGCTTTCGGGAAGGATGTTCAGCACGTTTATCTGCCGTATGATCTGCCCGATGCTCTCAACCGTTTcc                                                                                                                                          <  2:425572/150‑1 (MQ=255)
             tGCGCGCGCTGCGTCATCGTTATCCTGATTTACCGATTACCGTAACAACCATGACGCCAACCGGTTCGGAGCGCGTACAATCGGCTTTCGGGAAGGATGTTCAGCACGTTTATCTGCCGTATGATCTGCCCGATGCACTCAACCGTTTcc                                                                                                                                          <  2:2755611/150‑1 (MQ=255)
               cgcgcgcTGCGTCATCGTTATCCTGATTTACCGATTACCGTAACAACCATGACGCCAACCGGTTCGGAGCGCGTACAATCGGCTTTCGGGAAGGATGTTCAGCACGTTTATCTGCCGTATGATCTGCCCGATGCTCTCAACCGTTTCCTg                                                                                                                                        <  1:3830948/150‑1 (MQ=255)
               cgcgcgcTGCGTCATCGTTATCCTGATTTACCGATTACCGTAACAACCATGACGCCAACCGGTTCGGAGCGCGTACAATCGGCTTTCGGGAAGGATGTTCAGCACGTTTATCTGCCGTATGATCTGCCCGATGCTCTCAACCGTTTCCTg                                                                                                                                        <  1:3830683/150‑1 (MQ=255)
               cgcgcgcTGCGTCATCGTTATCCTGATTTACCGATTACCGTAACAACCATGACGCCAACCGGTTCGGAGCGCGTACAATCGGCTTTCGGGAAGGATGTTCAGCACGTTTATCTGCCGTATGATCTGCCCGATGCACTCAACCGTTTCCTg                                                                                                                                        <  2:1213168/150‑1 (MQ=255)
                     cTGCGTCATCGTTATCCTGATTTACCGATTACCGTAACAACCATGACGCCAACCGGTTCGGAGCGCGTACAATCGGCTTTCGGGAAGGATGTTCAGCACGTTTATCTGCCGTATGATCTGCCCGATGCACTCAACCGTTTCCTGAATaaa                                                                                                                                  <  2:2637259/150‑1 (MQ=255)
                       gCGTCATCGTTATCCTGATTTACCGATTACCGTAACAACCATGACGCCAACCGGTTCGGAGCGCGTACAATCGGCTTTCGGGAAGGATGTTCAGCACGTTTATCTGCCGTATGATCTGCCCGATGCTCTCAACCGTTTCCTGAATAAAGt                                                                                                                                <  1:4529194/150‑1 (MQ=255)
                        cGTCATCGTTATCCTGATTTACCGATTACCGTAACAACCATGACGCCAACCGGTTCGGAGCGCGTACAATCGGCTTTCGGGAAGGATGTTCAGCACGTTTATCTGCCGTATGATCTGCCCGATGCACTCAACCGTTTCCTGAATAAAGTc                                                                                                                               >  1:3306297/1‑150 (MQ=255)
                        cGTCATCGTTATCCTGATTTACCGATTACCGTAACAACCATGACGCCAACCGGTTCGGAGCGCGTACAATCGGCTTTCGGGAAGGATGTTCAGCACGTTTATCTGCCGTATGATCTGCCCGATGCACTCAACCGTTTCCTGAATAAAGTc                                                                                                                               <  2:1044477/150‑1 (MQ=255)
                           cATCGTTATCCTGATTTACCGATTACCGTAACAACCATGACGCCAACCGGTTCGGAGCGCGTACAATCGGCTTTCGGGAAGGATGTTCAGCACGTTTATCTGCCGTATGATCTGCCCGATGCTCTCAACCGTTTCCTGAATAAAGTCGAc                                                                                                                            <  2:3838106/150‑1 (MQ=255)
                            aTCGTTATCCTGATTTACCGATTACCGTAACAACCATGACGCCAACCGGTTCGGAGCGCGTACAATCGGCTTTCGGGAAGGATGTTCAGCACGTTTATCTGCCGTATGATCTGCCCGATGCACTCAACCGTTTCCTGAATAAAGTCGAcc                                                                                                                           >  2:3095483/1‑150 (MQ=255)
                                 tATCCTGATTTACCGATTACCGTAACAACCATGACGCCAACCGGTTCGGAGCGCGTACAATCGGCTTTCGGGAAGGATGTTCAGCACGTTTATCTGCCGTATGATCTGCCCGATGCACTCAACCGTTTCCTGAATAAAGTCGACCCTaaa                                                                                                                      >  1:721610/1‑150 (MQ=255)
                                   tCCTGATTTACCGATTACCGTAACAACCATGACGCCAACCGGTTCGGAGCGCGTACAATCGGCTTTCGGGAAGGATGTTCAGCACGTTTATCTGCCGTATGATCTGCCCGATGCACTCAACCGTTTCCTGAATAAAGTCGACCCTAAACt                                                                                                                    >  1:1055715/1‑150 (MQ=255)
                                     cTGATTTACCGATTACCGTAACAACCATGACGCCAACCGGTTCGGAGCGCGTACAATCGGCTTTCGGGAAGGATGTTCAGCACGTTTATCTGCCGTATGATCTGCCCGATGCACTCAACCGTTTCCTGAATAAAGTCGACCCTACACTgg                                                                                                                  <  2:1518047/150‑1 (MQ=255)
                                      tGATTTACCGATTACCGTAACAACCATGACGCCAACCGGTTCGGAGCGCGTACAATCGGCTTTCGGGAAGGATGTTCAGCACGTTTATCTGCCGTATGATCTGCCCGATGCTCTCAACCGTTTCCTGAATAAAGTCGACCCTAAACTGgt                                                                                                                 <  1:17354/150‑1 (MQ=255)
                                        aTTTACCGATTACCGTAACAACCATGACGCCAACCGGTTCGGAGCGCGTACAATCGGCTTTCGGGAAGGATGTTCAGCACGTTTATCTGCCGTATGATCTGCCCGATGCACTCCACCGTTTCCTGAATAAAGTCGACCCTAAACTGgtgt                                                                                                               >  2:2436197/1‑150 (MQ=255)
                                        aTTTACCGATTACCGTAACAACCATGACGCCAACCGGTTCGGAGCGCGTACAATCGGCTTTCGGGAAGGATGTTCAGCACGTTTATCTGCCGTATGATCTGCCCGATGCACTCAACCGTTTCCTGAATAAAGTCGACCCTAAACTGgtgt                                                                                                               >  1:1931344/1‑150 (MQ=255)
                                          ttACCGATTACCGTAACAACCATGACGCCAACCGGTTCGGAGCGCGTACAATCGGCTTTCGGGAAGGATGTTCAGCACGTTTATCTGCCGTATGATCTGCCCGATGCTCTCAACCGTTTCCTGAATAAAGTCGACCCTAAACTGGTGTTg                                                                                                             >  1:3466167/1‑150 (MQ=255)
                                          ttACCGATTACCGTAACAACCATGACGCCAACCGGTTCGGAGCGCGTACAATCGGCTTTCGGGAAGGATGTTCAGCACGTTTATCTGCCGTATGATCTGCCCGATGCACTCAACCGTTTCCTGAATAAAGTCGACCCTAAACTGGTGTTg                                                                                                             <  1:1160410/150‑1 (MQ=255)
                                              cGATTACCGTAACAACCATGACGCCAACCGGTTCGGAGCGCGTACAATCGGCTTTCGGGAAGGATGTTCAGCACGTTTATCTGCCGTATGATCTGCCCGATGCTCTCAACCGTTTCCTGAATAAAGTCGACCCTAAACTGGTGTTGATTa                                                                                                         <  1:1636299/150‑1 (MQ=255)
                                                  tACCGTAACAACCATGACGCCAACCGGTTCGGAGCGCGTACAATCGGCTTTCGGGAAGGATGTTCAGCACGTTTATCTGCCGTATGATCTGCCCGATGCACTCAACCGTTTCCTGAATAAAGTCGACCCTAAACTGGTGTTGATTATGGa                                                                                                     <  1:2881572/150‑1 (MQ=255)
                                                     cGTAACAACCATGACGCCAACCGGTTCGGAGCGCGTACAATCGGCTTTCGGGAAGGATGTTCAGCACGTTTATCTGCCGTATGATCTGCCCGATGCACTCAACCGTTTCCTGAATAAAGTCGACCCTAAACTGGTGTTGATTATGGAAAc                                                                                                  >  1:2754148/1‑150 (MQ=255)
                                                      gTAACAACCATGACGCCAACCGGTTCGGAGCGCGTACAATCGGCTTTCGGGAAGGATGTTCAGCACGTTTATCTGCCGTATGATCTGCCCGATGCACTCAACCGTTTCCTGAATAAAGTCGACCCTAAACTGGTGTTGATTATGGAAAcc                                                                                                 <  2:2537040/150‑1 (MQ=255)
                                                          caacCATGACGCCAACCGGTTCGGAGCGCGTACAATCGGCTTTCGGGAAGGATGTTCAGCACGTTTATCTGCCGTATGATCTGCCCGATGCACTCAACCGTTTCCTGAATAAAGTCGACCCTAAACTGGTGTTGATTATGGAAACCGAAc                                                                                             <  1:2279419/150‑1 (MQ=255)
                                                          caacCATGACGCCAACCGGTTCGGAGCGCGTACAATCGGCTTTCGGGAAGGATGTTCAGCACGTTTATCTGCCGTATGATCTGCCCGATGCACTCAACCGTTTCCTGAATAAAGTCGACCCTAAACTGGTGTTGATTATGGAAACCGAAc                                                                                             >  1:4217493/1‑150 (MQ=255)
                                                          caacCATGACGCCAACCGGTTCGGAGCGCGTACAATCGGCTTTCGGGAAGGATGTTCAGCACGTTTATCTGCCGTATGATCTGCCCGATGCACTCAACCGTTTCCTGAATAAAGTCGACCCTAAACTGGTGTTGATTATGGAAACCGAAc                                                                                             >  1:1360704/1‑150 (MQ=255)
                                                          caacCATGACGCCAACCGGTTCGGAGCGCGTACAATCGGCTTTCGGGAAGGATGTTCAGCACGTTTATCTGCCGTATGATCTGCCCGATGCACTCAACCGTTTCCTGAATAAAGTCGACCCTAAACTGGTGTTGATTATGGAAACCGAAc                                                                                             >  1:508601/1‑150 (MQ=255)
                                                          caacCATGACGCCAACCGGTTCGGAGCGCGTACAATCGGCTTTCGGGAAGGATGTTCAGCACGTTTATCTGCCGTATGATCTGCCCGATGCACTCAACCGTTTCCTGAATAAAGTCGACCCTAAACTGGTGTTGATTATGGAAACCGAAc                                                                                             >  1:355543/1‑150 (MQ=255)
                                                           aacCATGACGCCAACCGGTTCGGAGCGCGTACAATCGGCTTTCGGGAAGGATGTTCAGCACGTTTATCTGCCGTATGATCTGCCCGATGCACTCAACCGTTTCCTGAATAAAGTCGACCCTAAACTGGTGTTGATTATGGAAACCGAACt                                                                                            >  1:508926/1‑150 (MQ=255)
                                                            acCATGACGCCAACCGGTTCGGAGCGCGTACAATCGGCTTTCGGGAAGGATGTTCAGCACGTTTATCTGCCGTATGATCTGCCCGATGCTCTCAACCGTTTCCTGAATAAAGTCGACCCTAAACTGGTGTTGATTATGGAAACCGAACTa                                                                                           >  2:2611031/1‑150 (MQ=255)
                                                            acCATGACGCCAACCGGTTCGGAGCGCGTACAATCGGCTTTCGGGAAGGATGTTCAGCACGTTTATCTGCCGTATGATCTGCCCGATGCACTCAACCGTTTCCTGAATAAAGTCGACCCTAAACTGGTGTTGATTATGGAAACCGAACTa                                                                                           <  2:4391905/150‑1 (MQ=255)
                                                             ccATGACGCCAACCGGTTCGGAGCGCGTACAATCGGCTTTCGGGAAGGATGTTCAGCACGTTTATCTGCCGTATGATCTGCCCGATGCACTCAACCGTTTCCTGAATAAAGTCGACCCTAAACTGGTGTTGATTATGGAAACCGAACTAt                                                                                          <  1:2680720/150‑1 (MQ=255)
                                                              cATGACGCCAACCGGTTCGGAGCGCGTACAATCGGCTTTCGGGAAGGATGTTCAGCACGTTTATCTGCCGTATGATCTGCCCGATGCTCTCAACCGTTTCCTGAATAAAGTCGACCCTAAACTGGTGTTGATTATGGAAACCGAACTATg                                                                                         >  1:3710340/1‑150 (MQ=255)
                                                                    gCCAACCGGTTCGGAGCGCGTACAATCGGCTTTCGGGAAGGATGTTCAGCACGTTTATCTGCCGTATGATCTGCCCGATGCACTCAACCGTTTCCTGAATAAAGTCGACCCTAAACTGGTGTTGATTATGGAAACCGAACTATGGCCTaa                                                                                   >  2:1783185/1‑150 (MQ=255)
                                                                     ccAACCGGTTCGGAGCGCGTACAATCGGCTTTCGGGAAGGATGTTCAGCACGTTTATCTGCCGTATGATCTGCCCGATGCACTCAACCGTTTCCTGAATAAAGTCGACCCTAAACTGGTGTTGATTATGGAAACCGAACTATGGCCTAAc                                                                                  <  2:4039421/150‑1 (MQ=255)
                                                                      cAACCGGTTCGGAGCGCGTACAATCGGCTTTCGGGAAGGATGTTCAGCACGTTTATCTGCCGTATGATCTGCCCGATGCACTCAACCGTTTCCTGAATAAAGTCGACCCTAAACTGGTGTTGATTATGGAAACCGAACTATGGCCTAAcc                                                                                 >  2:3940538/1‑150 (MQ=255)
                                                                         ccGGTTCGGAGCGCGTACAATCGGCTTTCGGGAAGGATGTTCAGCACGTTTATCTGCCGTATGATCTGCCCGATGCACTCAACCGTTTCCTGAATAAAGTCGACCCTAAACTGGTGTTGATTATGGAAACCGAACTATGGCCTAACCTGa                                                                              <  2:369351/150‑1 (MQ=255)
                                                                           ggTTCGGAGCGCGTACAATCGGCTTTCGGGAAGGATGTTCAGCACGTTTATCTGCCGTATGATCTGCCCGATGCACTCAACCGTTTCCTGAATAAAGTCGACCCTAAACTGGTGTTGATTATGGAAACCGAACTATGGCCTAACCTGAtt                                                                            >  1:1570805/1‑150 (MQ=255)
                                                                            gTTCGGAGCGCGTACAATCGGCTTTCGGGAAGGATGTTCAGCACGTTTATCTGCCGTATGATCTGCCCGATGCACTCAACCGTTTCCTGAATAAAGTCGACCCTAAACTGGTGTTGATTATGGAAACCGAACTATGGCCTAACCTGATTg                                                                           <  1:4335833/150‑1 (MQ=255)
                                                                             ttCGGAGCGCGTACAATCGGCTTTCGGGAAGGATGTTCAGCACGTTTATCTGCCGTATGATCTGCCCGATGCACTCAACCGTTTCCTGAATAAAGTCGACCCTAAACTGGTGTTGATTATGGAAACCGAACTATGGCCTAACCTGATTgc                                                                          >  2:3318289/1‑150 (MQ=255)
                                                                               cGGAGCGCGTACAATCGGCTTTCGGGAAGGATGTTCAGCACGTTTATCTGCCGTATGATCTGCCCGATGCTCTCAACCGTTTCCTGAATAAAGTCGACCCTAAACTGGTGTTGATTATGGAAACCGAACTATGGCCTAACCTGATTgcgg                                                                        >  2:2610187/1‑150 (MQ=255)
                                                                                  aGCGCGTACAATCGGCTTTCGGGAAGGATGTTCAGCACGTTTATCTGCCGTATGATCTGCCCGATGCTCTCAACCGTTTCCTGAATAAAGTCGACCCTAAACTGGTGTTGATTATGGAAACCGAACTATGGCCTAACCTGATTGCGgcgc                                                                     >  2:1420181/1‑150 (MQ=255)
                                                                                  aGCGCGTACAATCGGCTTTCGGGAAGGATGTTCAGCACGTTTATCTGCCGTATGATCTGCCCGATGCACTCAACCGTTTCCTGAATAAAGTCGACCCTAAACTGGTGTTGATTATGGAAACCGAACTATGGCCTAACCTGATTGCGgcgc                                                                     >  1:3253355/1‑150 (MQ=255)
                                                                                   gcgcGTACAATCGGCTTTCGGGAAGGATGTTCAGCACGTTTATCTGCCGTATGATCTGCCCGATGCACTCAACCGTTTCCTGAATAAAGTCGACCCTAAACTGGTGTTGATTATGGAAACCGAACTATGGCCTAACCTGATTGCGGCGCt                                                                    <  2:3470884/150‑1 (MQ=255)
                                                                                   gcgcGTACAATCGGCTTTCGGGAAGGATGTTCAGCACGTTTATCTGCCGTATGATCTGCCCGATGCACTCAACCGTTTCCTGAATAAAGTCGACCCTAAACTGGTGTTGATTATGGAAACCGAACTATGGCCTAACCTGATTGCGGCGCt                                                                    <  2:1005439/150‑1 (MQ=255)
                                                                                    cgcgTACAATCGGCTTTCGGGAAGGATGTTCAGCACGTTTATCTGCCGTATGATCTGCCCGATGCACTCAACCGTTTCCTGAATAAAGTCGACCCTAAACTGGTGTTGATTATGGAAACCGAACTATGGCCTAACCTGATTGCGGCGCTa                                                                   <  2:3980385/150‑1 (MQ=255)
                                                                                    cgcgTACAATCGGCTTTCGGGAAGGATGTTCAGCACGTTTATCTGCCGTATGATCTGCCCGATGCACTCAACCGTTTCCTGAATAAAGTCGACCCTAAACTGGTGTTGATTATGGAAACCGAACTATGGCCTAACCTGATTGCGGCGCTa                                                                   <  2:727701/150‑1 (MQ=255)
                                                                                    cgcgTACAATCGGCTGTCGGGAAGGATGTTCAGCACGTTTATCTGCCGTATGATCTGCCCGATGCACTCCACCGTTTCCTGAATAAAGTCGACCCTAAACTGGTGTTGATTATGGAAACCGAACTATGGCCTAACCTGATTGCGGCGCTa                                                                   <  2:1605917/150‑1 (MQ=255)
                                                                                    cgcgGACAATCGGCTATCGGGAAGGAAGTTCAGCACGTTTAACTGCCGTATGATCTGCCCGATGCACTCAACCGTTACCTGAATAAAGTCGAACCTAAACTGGAGTGGATTATGGCAACCGAACTATGGCCTAACCTGATTGCGGCGCTa                                                                   >  2:937662/1‑150 (MQ=255)
                                                                                     gcgTACAATCGGCTTTCGGGAAGGATGTTCAGCACGTTTATCTGCCGTATGATCTGCCCGATGCACTCAACCGTTTCCTGAATAAAGTCGACCCTAAACTGGTGTTGATTATGGAAACCGAACTATGGCCTAACCTGATTGCGGCGCTAc                                                                  <  2:1055724/150‑1 (MQ=255)
                                                                                       gTACAATCGGCTTTCGGGAAGGATGTTCAGCACGTTTATCTGCCGTATGATCTGCCCGATGCACTCAACCGTTTCCTGAATAAAGTCGACCCTAAACTGGTGTTGATTATGGAAACCGAACTATGGCCTAACCTGATTGCGGCGCTACAt                                                                >  1:402905/1‑150 (MQ=255)
                                                                                       gTACAATCGGCTTTCGGGAAGGATGTTCAGCACGTTTATCTGCCGTATGATCTGCCCGATGCACTCAACCGTTTCCTGAATAAAGTCGACCCTAAACTGGTGTTGATTATGGAAACCGAACTATGGCCTAACCTGATTGCGGCGCTACAt                                                                <  1:3729799/150‑1 (MQ=255)
                                                                                          cAATCGGCTTTCGGGAAGGATGTTCAGCACGTTTATCTGCCGTATGATCTGCCCGATGCACTCAACCGTTTCCTGAATAAAGTCGACCCTAAACTGGTGTTGATTATGGAAACCGAACTATGGCCTAACCTGATTGCGGCGCTACATaaa                                                             >  1:1439557/1‑150 (MQ=255)
                                                                                            aTCGGCTTTCGGGAAGGATGTTCAGCACGTTTATCTGCCGTATGATCTGCCCGATGCACTCAACCGTTTCCTGAATAAAGTCGACCCTAAACTGGTGTTGATTATGGAAACCGAACTATGGCCTAACCTGATTGCGGCGCTACATAAACg                                                           <  1:2550691/150‑1 (MQ=255)
                                                                                             tCGGCTTTCGGGAAGGATGTTCAGCACGTTTATCTGCCGTATGATCTGCCCGATGCACTCAACCGTTTCCTGAATAAAGTCGACCCTAAACTGGTGTTGATTATGGAAACCGAACTATGGCCTAACCTGATTGCGGCGCTACATAAACGt                                                          <  2:1273591/150‑1 (MQ=255)
                                                                                              cGGCTTTCGGGAAGGATGTTCAGCACGTTTATCTGCCGTATGATCTGCCCGATGCACTCAACCGTTTCCTGAATAAAGTCGACCCTAAACTGGTGTTGATTATGGAAACCGAACTATGGCCTAACCTGATTGCGGCGCTACATAAACGTa                                                         <  2:4103606/150‑1 (MQ=255)
                                                                                               ggCTTTCGGGAAGGATGTTCAGCACGTTTATCTGCCGTATGATCTGCCCGATGCACTCAACCGTTTCCTGAATAAAGTCGACCCTAAACTGGTGTTGATTATGGAAACCGAACTATGGCCTAACCTGATTGCGGCGCTACATAAACGTaa                                                        >  1:1881736/1‑150 (MQ=255)
                                                                                               ggCTTTCGGGAAGGATGTTCAGCACGTTTATCTGCCGTATGATCTGCCCGATGCACTCAACCGTTTCCTGAATAAAGTCGACCCTAAACTGGTGTTGATTATGGAAACCGAACTATGGCCTAACCTGATTGCGGCGCTACATAAACGTaa                                                        <  1:1631468/150‑1 (MQ=255)
                                                                                                 cTTTCGGGAAGGATGTTCAGCACGTTTATCTGCCGTATGATCTGCCCGATGCACTCAACCGTTTCCTGAATAAAGTCGACCCTAAACTGGTGTTGATTATGGAAACCGAACTATGGCCTAACCTGATTGCGGCGCTACATAAACGTaaaa                                                      >  2:3507927/1‑150 (MQ=255)
                                                                                                     cGGGAAGGATGTTCAGCACGTTTATCTGCCGTATGATCTGCCCGATGCACTCAACCGTTTCCTGAATAAAGTCGACCCTAAACTGGTGTTGATTATGGAAACCGAACTATGGCCTAACCTGATTGCGGCGCTACATAAACGTAAAATTcc                                                  >  2:4530772/1‑150 (MQ=255)
                                                                                                     cGGGAAGGATGTTCAGCACGTTTATCTGCCGTATGATCTGCCCGATGCACTCAACCGTTTCCTGAATAAAGTCGACCCTAAACTGGTGTTGATTATGGAAACCGAACTATGGCCTAACCTGATTGCGGCGCTACATAAACGTAAAATTcc                                                  >  2:3207448/1‑150 (MQ=255)
                                                                                                     cGGGAAGGATGTTCAGCACGTTTATCTGCCGTATGATCTGCCCGATGCACTCAACCGTTTCCTGAATAAAGTCGACCCTAAACTGGTGTTGATTATGGAAACCGAACTATGGCCTAACCTGATTGCGGCGCTACATAAACGTAAAATTcc                                                  >  2:4191024/1‑150 (MQ=255)
                                                                                                      gggAAGGATGTTCAGCACGTTTATCTGCCGTATGATCTGCCCGATGCACTCAACCGTTTCCTGAATAAAGTCGACCCTAAACTGGTGTTGATTATGGAAACCGAACTATGGCCTAACCTGATTGCGGCGCTACATAAACGTAAAATTCCg                                                 >  2:482599/1‑150 (MQ=255)
                                                                                                       ggAAGGATGTTCAGCACGTTTATCTGCCGTATGATCTGCCCGATGCTCTCAACCGTTTCCTGAATAAAGTCGACCCTAAACTGGTGTTGATTATGGAAACCGAACTATGGCCTAACCTGATTGCGGCGCTACATAAACGTAAAATTCCGc                                                >  1:2155271/1‑150 (MQ=255)
                                                                                                       ggAAGGATGTTCAGCACGTTTATCTGCCGTATGATCTGCCCGATGCACTCAACCGTTTCCTGAATAAAGTCGACCCTAAACTGGTGTTGATTATGGAAACCGAACTATGGCCTAACCTGATTGCGGCGCTACATAAACGTAAAATTCCGc                                                >  1:2312762/1‑150 (MQ=255)
                                                                                                       ggAAGGATGTTCAGCACGTTTATCTGCCGTATGATCTGCCCGATGCACTCAACCGTTTCCTGAATAAAGTCGACCCTAAACTGGTGTTGATTATGGAAACCGAACTATGGCCTAACCTGATTGCGGCGCTACATAAACGTAAAATTCCGc                                                >  1:1086324/1‑150 (MQ=255)
                                                                                                             aTGTTCAGCACGTTTATCTGCCGTATGATCTGCCCGATGCTCTCAACCGTTTCCTGAATAAAGTCGACCCTAAACTGGTGTTGATTATGGAAACCGAACTATGGCCTAACCTGATTGCGGCGCTACATAAACGTAAAATTCCGCTGGTGa                                          >  1:1133347/1‑150 (MQ=255)
                                                                                                              tGTTCAGCACGTTTATCTGCCGTATGATCTGCCCGATGCACTCAACCGTTTCCTGAATAAAGTCGACCCTAAACTGGTGTTGATTATGGAAACCGAACTATGGCCTAACCTGATTGCGGCGCTACATAAACGTAAAATTCCGCTGGTGAt                                         <  1:1147059/150‑1 (MQ=255)
                                                                                                              tGTTCAGCACGTTTATCTGCCGTATGATCTGCCCGATGCACTCAACCGTTTCCTGAATAAAGTCGACCCTAAACTGGTGTTGATTATGGAAACCGAACTATGGCCTAACCTGATTGCGGCGCTACATAAACGTAAAATTCCGCTGGTGAt                                         >  1:147894/1‑150 (MQ=255)
                                                                                                              tGTTCAGCACGTTTATCTGCCGTATGATCTGCCCGATGCACTCAACCGTTTCCTGAATAAAGTCGACCCTAAACTGGTGTTGATTATGGAAACCGAACTATGGCCTAACCTGATTGCGGCGCTACATAAACGTAAAATTCCGCTGGTGAt                                         <  1:862930/150‑1 (MQ=255)
                                                                                                                   aGCACGTTTATCTGCCGTATGATCTGCCCGATGCACTCAACCGTTTCCTGAATAAAGTCGACCCTAAACTGGTGTTGATTATGGAAACCGAACTATGGCCTAACCTGATTGCGGCGCTACATAAACGTAAAATTCCGCTGGTGATCGCTa                                    >  2:637154/1‑150 (MQ=255)
                                                                                                                      aCGTTTATCTGCCGTATGATCTGCCCGATGCACTCAACCGTTTCCTGAATAAAGTCGACCCTAAACTGGTGTTGATTATGGAAACCGAACTATGGCCTAACCTGATTGCGGCGCTACATAAACGTAAAATTCCGCTGGTGATCGCTAAcg                                 <  1:3505212/150‑1 (MQ=255)
                                                                                                                       cGTTTATCTGCCGTATGATCTGCCCGATGCACTCAACCGTTTCCTGAATAAAGTCGACCCTAAACTGGTGTTGATTATGGAAACCGAACTATGGCCTAACCTGATTGCGGCGCTACATAAACGTAAAATTCCGCTGGTGATCGCTAAcgc                                >  1:3086644/1‑150 (MQ=255)
                                                                                                                       cGTTTATCTGCCGTATGATCTGCCCGATGCACTCAACCGTTTCCTGAATAAAGTCGACCCTAAACTGGTGTTGATTATGGAAACCGAACTATGGCCTAACCTGATTGCGGCGCTACATAAACGTAAAATTCCGCTGGTGATCGCTAAcgc                                >  1:822760/1‑150 (MQ=255)
                                                                                                                        gTTTATCTGCCGTATGATCTGCCCGATGCACTCAACCGTTTCCTGAATAAAGTCGACCCTAAACTGGTGTTGATTATGGAAACCGAACTATGGCCTAACCTGATTGCGGCGCTACATAAACGTAAAATTCCGCTGGTGATCGCTAAcgcg                               <  1:4085192/150‑1 (MQ=255)
                                                                                                                        gTTTATCTGCCGTATGATCTGCCCGATGCACTCAACCGTTTCCTGAATAAAGTCGACCCTAAACTGGTGTTGATTATGGAAACCGAACTATGGCCTAACCTGATTGCGGCGCTACATAAACGTAAAATTCCGCTGGTGATCGCTAAcgcg                               >  1:447660/1‑150 (MQ=255)
                                                                                                                          ttATCTGCCGTATGATCTGCCCGATGCACTCAACCGTTTCCTGAATAAAGTCGACCCTAAACTGGTGTTGATTATGGAAACCGAACTATGGCCTAACCTGATTGCGGCGCTACATAAACGTAAAATTCCGCTGGTGATCGCTAAcgcgcg                             >  1:1010962/1‑150 (MQ=255)
                                                                                                                            aTCTGCCGTATGATCTGCCCGATGCTCTCAACCGTTTCCTGAATAAAGTCGACCCTAAACTGGTGTTGATTATGGAAACCGAACTATGGCCTAACCTGATTGCGGCGCTACATAAACGTAAAATTCCGCTGGTGATCGCTAACGCGCGAc                           <  2:1910329/150‑1 (MQ=255)
                                                                                                                            aTCTGCCGTATGATCTGCCCGATGCACTCAACCGTTTCCTGAATAAAGTCGACCCTAAACTGGTGTTGATTATGGAAACCGAACTATGGCCTAACCTGATTGCGGCGCTACATAAACGTAAAATTCCGCTGGTGATCGCTAACGCGCGAc                           >  1:1705375/1‑150 (MQ=255)
                                                                                                                             tCTGCCGTATGATCTGCCCGATGCACTCAACCGTATCCTGAAAAAAGTCGACCCTAAACTGGTGATCATTATGGAAACCGAACAATGGCCTAACCTGATTGCGGCGCTACATAAACGAAAAATTCCGCTGGTGATCGCTAACGCGCGAct                          >  1:2014597/1‑150 (MQ=255)
                                                                                                                              cTGCCGTATGATCTGCCCGATGCACTCAACCGTTTCCTGAATAAAGTCGACCCTAAACTGGTGTTGATTATGGAAACCGAACTATGGCCTAACCTGATTGCGGCGCTACATAAACGTAAAATTCCGCTGGTGATCGCTAACGCGCGActc                         >  1:1717393/1‑150 (MQ=255)
                                                                                                                               tGCCGTATGATCTGCCCGATGCACTCAACCGTTTCCTGAATAAAGTCGACCCTAAACTGGTGTTGATTATGGAAACCGAACTATGGCCTAACCTGATTGCGGCGCTACATACACGTAAAATTCCGCTGGTGATCGCTAACGCGCGActct                        >  2:4237662/1‑150 (MQ=255)
                                                                                                                               tGCCGTATGATCTGCCCGATGCACTCAACCGTTTCCTGAATAAAGTCGACCCTAAACTGGTGTTGATTATGGAAACCGAACTATGGCCTAACCTGATTGCGGCGCTACATAAACGTAAAATTCCGCTGGTGATCGCTAACGCGCGActct                        <  2:4301202/150‑1 (MQ=255)
                                                                                                                                  cGTATGATCTGCCCGATGCTCTCAACCGTTTCCTGAATAAAGTCGACCCTAAACTGGTGTTGATTATGGAAACCGAACTATGGCCTAACCTGATTGCGGCGCTACATAAACGTAAAATTCCGCTGGTGATCGCTAACGCGCGACTCTCTg                     <  2:1566409/150‑1 (MQ=255)
                                                                                                                                  cGTATGATCTGCCCGATGCACTCAACCGTTTCCTGAATAAAGTCGACCCTAAACTGGTGTTGATTATGGAAACCGAACTATGGCCTAACCTGATTGCGGCGCTACATAAACGTAAAATTCCGCTGGTGATCGCTAACGCGCGACTCTCTg                     <  2:299992/150‑1 (MQ=255)
                                                                                                                                      tGATCTGCCCGATGCACTCAACCGTTTCCTGAATAAAGTCGACCCTAAACTGGTGTTGATTATGGAAACCGAACTATGGCCTAACCTGATTGCGGCGCTACATAAACGTAAAATTCCGCTGGTGATCGCTAACGCGCGACTCTCTGCCCg                 <  1:3095483/150‑1 (MQ=255)
                                                                                                                                        aTCTGCCCGATGCTCTCAACCGTTTCCTGAATAAAGTCGACCCTAAACTGGTGTTGATTATGGAAACCGAACTATGGCCTAACCTGATTGCGGCGCTACATAAACGTAAAATTCCGCTGGTGATCGCTAACGCGCGACTCTCTGCCCGCt               >  2:2625378/1‑150 (MQ=255)
                                                                                                                                         tCTGCCCGATGCTCTCAACCGTTTCCTGAATAAAGTCGACCCTAAACTGGTGTTGATTATGGAAACCGAACTATGGCCTAACCTGATTGCGGCGCTACATAAACGTAAAATTCCGCTGGTGATCGCTAACGCGCGACTCTCTGCCCGCTc              >  1:1305808/1‑150 (MQ=255)
                                                                                                                                         tCTGCCCGATGCACTCAACCGTTTCCTGAATAATGTCGACCCTAACCTGGTGTTGATTATGGAAACCGAACTATGGCCTAACCTGATTGCGGCGCTACATAAACGTAAAATTCCGCTGGTGATCGCTAACGCGCGACTCTCTGCCCGCTc              <  1:1995350/150‑1 (MQ=255)
                                                                                                                                         tCTGCCCGATGCACTCAACCGTTTCCTGAATAAAGTCGACCCTAAACTGGTGTTTATTATGGAAACCGAACTATGTCATAACCTGATTGCGGCGCTACATAAACGTAAAATTCCGCTGGTGATCGCTAACGCGCGACTCTCTGCCCGCTc              <  1:1777831/150‑1 (MQ=255)
                                                                                                                                         tCTGCCCGATGCACTCAACCGTTTCCTGAATAAAGTCGACCCTAAACTGGTGTTGATTATGGAAACCGAACTATGGCCTAACCTGATTGCGGCGCTACATAAACGTAAAATTCCGCTGGTGATCGCTAACGCGCGACTCTCTGCCCGCTc              <  1:4188968/150‑1 (MQ=255)
                                                                                                                                               cGATGCACTCAACCGTTTCCTGAATAAAGTCGACCCTAAACTGGTGTTGATTATGGAAACCGAACTATGGCCTAACCTGATTGCGGCGCTACATAAACGTAAAATTCCGCTGGTGATCGCTAACGCGCGACTCTCTGCCCGCTCGGCCGc        >  2:4006536/1‑150 (MQ=255)
                                                                                                                                                  tGCACTCAACCGTTTCCTGAATAAAGTCGACCCTAAACTGGTGTTGATTATGGAAACCGAACTATGGCCTAACCTGATTGCGGCGCTACATAAACGTAAAATTCCGCTGGTGATCGCTAACGCGCGACTCTCTGCCCGCTCGGCCGCAgg     <  1:249941/150‑1 (MQ=255)
                                                                                                                                                  tGCACTCAACCGTTTCCTGAATAAAGTCGACCCTAAACTGGTGTTGATTATGGAAACCGAACTATGGCCTAACCTGATTGCGGCGCTACATAAACGTAAAATTCCGCTGGTGATCGCTAACGCGCGACTCTCTGCCCGCTCGGCCGCAgg     <  2:1718318/150‑1 (MQ=255)
                                                                                                                                                  tGCACTCAACCGTTTCCTGAATAAAGTCGACCCTAAACTGGTGTTGATTATGGAAACCGAACTATGGCCTAACCTGATTGCGGCGCTACATAAACGTAAAATTCCGCTGGTGATCGCTAACGCGCGACTCTCTGCCCGCTCGGCCGCAgg     >  1:670945/1‑150 (MQ=255)
                                                                                                                                                    cACTCAACCGTTTCCTGAATAAAGTCGACCCTAAACTGGTGTTGATTATGGAAACCGAACTATGGCCTAACCTGATTGCGGCGCTACATAAACGTAAAATTCCGCTGGTGATCGCTAACGCGCGACTCTCTGCCCGCTCGGCCGCAGGtt   <  2:2258804/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
GCAATCCCGTTGGTGCGCGCGCTGCGTCATCGTTATCCTGATTTACCGATTACCGTAACAACCATGACGCCAACCGGTTCGGAGCGCGTACAATCGGCTTTCGGGAAGGATGTTCAGCACGTTTATCTGCCGTATGATCTGCCCGATGCACTCAACCGTTTCCTGAATAAAGTCGACCCTAAACTGGTGTTGATTATGGAAACCGAACTATGGCCTAACCTGATTGCGGCGCTACATAAACGTAAAATTCCGCTGGTGATCGCTAACGCGCGACTCTCTGCCCGCTCGGCCGCAGGTTA  >  CP009273/3802089‑3802387

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑