BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	3,822,339	A→C	10.0%	Q12P (CAA→CCA) ‡	xanP →	xanthine permease

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	3,822,339	0	A	C	10.0%	408.7 / 4.2	209	Q12P (CAA→CCA) ‡	xanP	xanthine permease
Reads supporting (aligned to +/- strand): ref base A (101/79); new base C (27/2); total (128/81)
Fisher's exact test for biased strand distribution p-value = 6.14e-05
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 214 total aligned reads displayed.

TATAAAAAAGTGCATTTATCTCATTTAAATATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACC‑GGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCGGTGATCACGCCAGCGCTATTAATCTGCCAGGCGCTGG  >  CP009273/3822192‑3822488

tataAAAAAGTGCATTTATCTCATTTAAATATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAAc                                                                                                                                                      >  2:268813/1‑150 (MQ=255)

   aaaaaaGTGCATTTATCTCATTTAAATATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAAAGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACC‑gg                                                                                                                                                  >  1:3640991/1‑150 (MQ=255)

    aaaaaGTGCATTTATCTCATTTATATATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCAAGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACC‑GGt                                                                                                                                                 <  2:2216760/150‑1 (MQ=255)

      aaaGTGCATTTATCTCATTTAAATATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACC‑GGTTg                                                                                                                                               <  2:2705326/150‑1 (MQ=255)

      aaaGTGCATTTATCTCATTTAAATATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACC‑GGTTg                                                                                                                                               <  1:3616222/150‑1 (MQ=255)

         gTGCATTTATCTCATTTAAATATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACC‑GGGTgcgc                                                                                                                                            >  1:2511830/1‑150 (MQ=255)

           gCATTTATCTCATTTAAATATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACC‑CGTTgcgccg                                                                                                                                          >  1:1340241/1‑148 (MQ=255)

           gCATTTATCTCATTTAAATATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCCTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCCACC‑GGGTGCGCAg                                                                                                                                          >  2:2802898/1‑150 (MQ=255)

             aTTTATCTCATTTAAATATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACC‑CGTTGCGCAGAc                                                                                                                                        >  2:389066/1‑150 (MQ=255)

              tttATCTCATTTAAATATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACC‑GGTTGCGCAGACt                                                                                                                                       <  2:688140/150‑1 (MQ=255)

                 aTCTCATTTAAATATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGACAAACC‑GGTTTCGCAGACTaaa                                                                                                                                    >  2:2919523/1‑150 (MQ=255)

                  tctcATTTAAATATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTGTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCCCCCTCGAGTCAGAAAATGCGCAACC‑GGTTGCGCAGACTCaaa                                                                                                                                   >  2:2417146/1‑150 (MQ=255)

                   ctcATTTAATTATTTAGATGAGGATGTGGCAAACGTTTGATGTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACC‑GGTTGCGCAGACTCaaaa                                                                                                                                  <  2:1650640/150‑1 (MQ=255)

                   ctcATTTAAATATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACC‑GGTTGCGCAGACTCaaaa                                                                                                                                  >  2:1490783/1‑150 (MQ=255)

                    tcATTTAAATATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCCCCC‑GGGTGCGCAGACACAAAAc                                                                                                                                 >  1:140987/1‑150 (MQ=255)

                      aTTTAAATATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCCACC‑CGTTGCGCACACACAAAAAAg                                                                                                                               >  1:2963175/1‑150 (MQ=255)

                       tttAAATATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACC‑CGGTGCGCAGACTCAAAAAAga                                                                                                                              >  1:3914957/1‑149 (MQ=255)

                           attatTTAGATGAGTATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAATTGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACC‑GGTTGCGCAGACTCAAAACAGCGAAc                                                                                                                          <  2:2969083/148‑1 (MQ=255)

                           attatTTAGATGAGGTTGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACC‑GGTTGCGCAGACTCAAAACAGCGAAc                                                                                                                          <  2:351701/148‑1 (MQ=255)

                                 tAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCCCCC‑CCTTGCGCACACACAAAACAGCCAACAGAttt                                                                                                                    >  1:1027303/1‑150 (MQ=255)

                                  aGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCACCC‑GGTTGCGCAGACTCAAAACAGCGAACTGATTTa                                                                                                                   >  2:4471254/1‑150 (MQ=255)

                                  aGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACC‑GGTTGCGCAGACTCAAAACAGCGAACTGATTTa                                                                                                                   >  2:1416805/1‑150 (MQ=255)

                                   gATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACC‑GGTTGCGCAGACTCAAAACAGCGAACTGATTTAc                                                                                                                  <  2:2764697/150‑1 (MQ=255)

                                    aTGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACC‑CGGTGCGCACAAACAAAACACCGAACAGAttttaa                                                                                                                 >  1:40158/1‑147 (MQ=255)

                                     tGAGGATGTGGCACACGTTTGCTTTTCTCTTTCGGTCAGCTAAAAAGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACG‑GGTTGCGCAGACTCAAAACAGCGAACTGATTTACCg                                                                                                                <  2:3519193/150‑1 (MQ=255)

                                     tGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAAAGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACC‑GGTTGCGCAGACTCAAAACAGCGAACTGATTTACCg                                                                                                                >  2:1057359/1‑150 (MQ=255)

                                      ttggATGTGGAAATCTTTTGCTTTTATCTTTCTGTCAGCTAAAATGCCCGTTTTGCTACCTGGGTTTTTTTTTCTTTGTTTTTTTCCACCCTCGAGACACAAAATGCCAAAGG‑TGTTGCCCAGACTCAAAACAGCGAACTGATTTACCGt                                                                                                               <  1:23976/148‑1 (MQ=255)

                                                  aaCGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACC‑GGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATAGt                                                                                                   >  2:1234498/1‑150 (MQ=255)

                                                   tcGTTTGCTTTTCTCTTTCGTTCAGATAAAATGCCCGCTTTTCTACCAGGTGTTTTTTTTCTTTGTTTTTTACCACCCTCGAGTCATAAAATGCGCACCG‑GGTGGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTc                                                                                                  <  1:2177427/149‑1 (MQ=255)

                                                    cGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACC‑GGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGtac                                                                                                 >  2:481880/1‑148 (MQ=255)

                                                    cGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACC‑GGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTcc                                                                                                 >  2:2929308/1‑150 (MQ=255)

                                                    cGTTTGCTTTTCTCTTTCGGTCAGCTAAAAAGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACC‑GGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCGTGAAGATCGTcc                                                                                                 >  2:3246385/1‑150 (MQ=255)

                                                          cTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACC‑GGTTGCGCAGACTCAAAACAGCGAACAGATTTAACGTGTTGTATATAGTCcgccgcg                                                                                           >  2:2320473/1‑149 (MQ=255)

                                                           ttttCTCTTTCGGACCTATAAATGGCCCTATTGGCTACCCCGGTTTTTTTTTCGTTGTGTTTTCCCCCCCTCTTGACCTAAATTGCCCACCC‑GGTCGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCtt                                                                                          <  1:986287/150‑1 (MQ=255)

                                                            tttCTGTTGCGGTCAGCTAAACGGCCCGGTTTGCTACCCCGGGTTTGTTTTCGTGGTCTGTTTCCCCCCTCGCGGCAGAAATGGCGCACCG‑GGGGGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTc                                                                                         <  1:3728563/150‑1 (MQ=255)

                                                            tttCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACC‑GGTTGCGCAGACTCAAAAAAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTc                                                                                         >  2:2261340/1‑150 (MQ=255)

                                                              tctcTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCCACC‑GGTTGCGCAGACTCAAAACAGCGAACAGATTTACAGTCTTTAAGATCGTCcgccgcgtact                                                                                       >  1:3692771/1‑145 (MQ=255)

                                                              tctcTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACC‑GGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCt                                                                                       >  2:1977801/1‑150 (MQ=255)

                                                              tctcTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACC‑GGTTGCGCAGACACAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCt                                                                                       >  1:1810419/1‑150 (MQ=255)

                                                              tctcTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACC‑CGTTGCGCAGACACAAAACACCCAACAGATTTACCGTCATGAAGAACGTCccccgcgacca                                                                                       >  1:2574385/1‑145 (MQ=255)

                                                                  tttCGGTCAGCTAAAATGCCCGCTTTCCTACCAGGTTTTTGTTTTCGTTTTCTGTTACCCCCCTCGATTCATAAAATGCGCAACC‑GGTGGCCCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCaaa                                                                                   <  1:1470949/150‑1 (MQ=255)

                                                                    tgggTCCGCTAAATGGCCGGCTTGGCTACCAGGGTTTTTTTTTCGTTGTGTGTGTCCCCCCCCGAGTCAGAAACGGCGCACGG‑GGGGGCCCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAAcc                                                                                 <  1:2324600/148‑1 (MQ=255)

                                                                    tCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGGGCACCC‑GGTTTGGCCGACTCAAAAAAGCGAAATGATTTACCGGCTTGACTTTAGTCCGCCCCTGCCTCAAAcc                                                                                 >  1:3503596/1‑150 (MQ=255)

                                                                    tCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCCACC‑GGTTGCGCACACAAAAAAAACCGAACTGATTTTACTAGTTTTAGAAACTACGCCGCTTCCTCAAAca                                                                                 >  1:3084859/1‑149 (MQ=255)

                                                                    tCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCCACC‑CGTTGCGCAGACTCAAAAAAGCGAACTGATTTACCGTCTTGTAGATAGTCCGCCGCTTCCTCACAcc                                                                                 >  2:1501367/1‑150 (MQ=255)

                                                                    tCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACC‑GGTTGCGCAGACTCAAAACAGCGAACAGATTTACCGTCTTGAAGATCGTCCGCCGCGTCCGCAAAcc                                                                                 >  1:304993/1‑150 (MQ=255)

                                                                     cGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACC‑GGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCGTGAAGATCGTCCGCCGCTTCCTCAAAccc                                                                                >  2:2379157/1‑150 (MQ=255)

                                                                         cAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAAAC‑CGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGTAGATCTTCCGCCGCTTCCACAAACCCTGtt                                                                            >  2:2579241/1‑150 (MQ=255)

                                                                             tAAAAAGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACC‑GGGTGCGCCGACTCAAAAAAGCGAACAGATTTAGCGTGTTGTAGATCGTCCGCCGCGTGCGCAAAACCAGtgtggc                                                                        >  1:3826262/1‑148 (MQ=255)

                                                                               aaaTGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCCACC‑GGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTgccgc                                                                      >  2:284315/1‑150 (MQ=255)

                                                                                aaagCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCCACC‑GGTTGCGCAGACACAAAAAAGCGAAATGATTTACAGTCGTTAAGATCGTCCGCCGCGTCCTCAAAACCTGTTTgccgcc                                                                     >  1:691182/4‑150 (MQ=255)

                                                                                aaTGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACC‑GGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTgccgcc                                                                     >  2:4288257/1‑150 (MQ=255)

                                                                                    cccGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACC‑GGGTGCGCAGACTCAAAAAAGCGAACAGATTTAACGTCTTGAAGATAGTCCACCGCATCCTCAAAACCAGTTTGCCGCCTGTc                                                                 >  1:851210/1‑150 (MQ=255)

                                                                                    cccGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCCCAACC‑GGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCACAAAACCTGTTTGCCCCCAGTc                                                                 >  2:4361807/1‑150 (MQ=255)

                                                                                     cctcTTTGCTACCAGTGTTTTGTTTTCGTTGTCTTTCACCCCCCTCTAGACACAAAAGGCGCAACC‑GGTTGCCCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCa                                                                <  1:4203238/147‑1 (MQ=255)

                                                                                      ggCTTTGCTACCTGGGGTTTGTTTTGGTTGTGTGTTACCACCCTCGAGTCAGAAAATGCGCACCG‑GGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAg                                                               <  1:1659479/149‑1 (MQ=255)

                                                                                      cGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACC‑GGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAg                                                               >  2:3017337/1‑150 (MQ=255)

                                                                                        cTTTGATACCCCGGGATTGTTTTCGATGTCTGTTTCCCCCCTCGAGTCAGAAAATGCGCAACC‑GGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCa                                                             <  2:4009087/150‑1 (MQ=255)

                                                                                         tttGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCCACC‑CGGTGCGCAGACACAAAACACCGAACAGATTTACCCTCATGAAGATCGTCCGCCCCGTCCACAAACCCGGTTTGCCGCCAGTCCGCAt                                                            >  2:421890/1‑150 (MQ=255)

                                                                                           tGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCCACC‑GGTTGCGCAGACTCAAAACAGCGAACGGATTTACCGTCATGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATct                                                          >  2:1845481/1‑150 (MQ=255)

                                                                                           tGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACC‑GGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATct                                                          <  2:921100/150‑1 (MQ=255)

                                                                                           tGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACC‑GGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCGGTTTGCCGCCTGTCAGCATct                                                          >  2:3856230/1‑150 (MQ=255)

                                                                                           tGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACC‑GGGTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTTTTTGCCGCCTGTCAGCATct                                                          <  2:1033226/150‑1 (MQ=255)

                                                                                                ccccGGGAGTGTTTTCGATTGCTGTTACCCCCCTCGAGTCAGAAATTGCGCACCG‑GGTGGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTgg                                                     <  1:3232439/147‑1 (MQ=255)

                                                                                                ccACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACC‑GGTTGCGCAGACTCAAAACAGCGAACTGATTTAACGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTAGCCGCCTGTCAGCATCTGCTgg                                                     >  2:2195744/1‑150 (MQ=255)

                                                                                                    gggATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCACCC‑GGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGAt                                                 <  2:4152074/150‑1 (MQ=255)

                                                                                                     gtaTTGTTTTCGATGTCTGTTTCCCCCCTCGAGACAGAAATGGCGCACCC‑GGTCGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCGTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATg                                                <  1:381321/148‑1 (MQ=255)

                                                                                                     ggATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCACCC‑GGTTGCGCCCACTCAAAACAGCGAACAGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCAGTTTGCCGCCAGTCAGCAACAGCTGGCGATg                                                >  1:3383512/1‑150 (MQ=255)

                                                                                                        tttttttCTTTGTCTGTTACCACCCTCGAGTCACAAATTGCGCAACC‑GGTCGCCCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTc                                             <  1:1980959/147‑1 (MQ=255)

                                                                                                        ttGTTTTCGATGTCTGTTCCCCCCCTCGAGTCAGAAATTGCGCACCC‑GGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTc                                             <  1:3741794/150‑1 (MQ=255)

                                                                                                         tGTTTTCTCTTTCTGTTCCCACCCGCGAGTCATAAAATGCGCACCG‑GGGTGCCCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCg                                            <  1:813626/150‑1 (MQ=255)

                                                                                                          gTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACC‑GGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGt                                           >  2:2540681/1‑150 (MQ=255)

                                                                                                          gTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACC‑GGTTGCGCAGACACAAAACAGCGAACTGTTTTAAAGTCTTTAATATCGTCCGCCGCTTCCTCAAACCCAGTTTTGCGCCGGTCAGCATCTGCTGGCGATGTTCGt                                           >  1:1098981/1‑150 (MQ=255)

                                                                                                           ttttCGATGTCTGTTTCCACCCTCGAGTCAGAAACTGCGCAACC‑GGTTGCGCTGACTCAAAACAGCGAAATGATTTACCGTCTTGAAGTTCGTCCGCCGCTTCCTCCAGCCCTGGTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGtt                                          >  2:1810991/1‑150 (MQ=255)

                                                                                                           ttttCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACC‑GGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCGCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGtt                                          >  2:1810881/1‑150 (MQ=255)

                                                                                                            tttCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACC‑GGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTg                                         >  2:4108805/1‑150 (MQ=255)

                                                                                                             ttCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCCACC‑GGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCGGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGc                                        >  2:961269/1‑150 (MQ=255)

                                                                                                             ttCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCCACC‑GGTTGCGCAGACACAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCATCCTCAAACCCGGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGc                                        >  2:808429/1‑150 (MQ=255)

                                                                                                                gATGTGTGTTTCCACCCTCGAGTCAGAAAATGCGCACCC‑GGTGGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCGGt                                     <  2:2319368/150‑1 (MQ=255)

                                                                                                                gATGTCTGTTTCCACCCTCGAGTCAGAAAAGGCGCCACC‑GGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCGGt                                     <  2:4173100/150‑1 (MQ=255)

                                                                                                                 aTGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCACCC‑GGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCGGTg                                    <  2:1850162/150‑1 (MQ=255)

                                                                                                                  tGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACC‑GGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTTCGGTGa                                   >  2:3769627/1‑150 (MQ=255)

                                                                                                                     ctgtcaccACCCTCGAGACCGAACATGCGCCACC‑GGGCGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCGGTGATCa                                <  1:1898075/144‑1 (MQ=255)

                                                                                                                      tGTTCCCCCCCTCGAGTCAGAAACTGCGCACCG‑GGGGGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCGGTGATCAc                               <  1:2939514/150‑1 (MQ=255)

                                                                                                                         ttCCACCCTCGAGTCAGAAAATGCGCAACC‑GGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCGGTGATCACGcc                            >  2:3314270/1‑150 (MQ=255)

                                                                                                                         ttCCACCCTCGAGTCAGAAAATGCGCAACC‑GGTTGCGCAGACACAAAACAGCGAACTGATTTACCGTCTTGAAGATAGTCCGCCGCGTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGAAGTTCGTTGCGGTGATCACGcc                            >  2:3209740/1‑150 (MQ=255)

                                                                                                                          tCCACCCGCGAGTCAGAAAATGCGCAACC‑GGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCGGTGATCACGCCa                           <  2:3392811/150‑1 (MQ=255)

                                                                                                                             aCCCGCGAGTCAGAAAATGCGCAACC‑TGTGGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCGGTGATCACGCCAgcg                        <  1:653781/150‑1 (MQ=255)

                                                                                                                                  cGAGTCAGAAATTGCGCAACC‑GGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCGGTGATCACGCCAGCGCTAtt                   <  2:36834/150‑1 (MQ=255)

                                                                                                                                      tCAGAAAAGGCGCACCC‑GGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCGGTGATCACGCCAGCGCTATTAATc               <  2:3154749/150‑1 (MQ=255)

                                                                                                                                       actaaatTGCGCAACG‑GGTGGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCGGTGATCACGCCAGCGCTATTAATCt              <  1:4379187/147‑1 (MQ=255)

                                                                                                                                        aGAAAATGCGCACCC‑GTTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCGGTGATCACGCCAGCGCTATTAATCTg             <  2:4529658/150‑1 (MQ=255)

                                                                                                                                        aGAAAATGCGCAACG‑GGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCGGTGATCACGCCAGCGCTATTAATCTg             <  2:1340241/150‑1 (MQ=255)

                                                                                                                                           ataggcgcAACG‑GGGGCCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCGGTGATCACGCCAGCGCTATTAATCTGCCa          <  1:1634254/146‑1 (MQ=255)

                                                                                                                                           aattGCGCACCG‑GGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCGGTGATCACGCCAGCGCTATTAATCTGCCa          <  1:2458852/147‑1 (MQ=255)

                                                                                                                                            atatgccAACCGGGTGGCCCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCGGTGATCACGCCAGCGCTATTAATCTGCCa          <  1:2703118/144‑1 (MQ=255)

                                                                                                                                             aggcgcAACC‑GGTGGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCATCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCGGTGATCACGCCAGCGCTATTAATCTGCCAgt        <  2:2602449/148‑2 (MQ=255)

                                                                                                                                             aTGCGCAACC‑GGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCGGTGATCACGCCAGCGCTATTAATCTGCCAgg        >  1:1401222/1‑150 (MQ=255)

                                                                                                                                                   aaCG‑GGTTGCCCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCGGTGATCACGCCAGCGCTATTAATCTGCCAGGCGCTgg  <  1:3391272/150‑1 (MQ=255)

                                                                                                                                                   aaCC‑GGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCGGTGATCACGCCAGCGCTATTAATCTGCCAGGCGCTgg  >  2:3834942/1‑150 (MQ=255)

TATAAAAAAGTGCATTTATCTCATTTAAATATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACC‑GGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCGGTGATCACGCCAGCGCTATTAATCTGCCAGGCGCTGG  >  CP009273/3822192‑3822488

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`