BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	3,822,345	T→G	9.7%	V14G (GTT→GGT)	xanP →	xanthine permease

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	3,822,345	0	T	G	9.7%	451.4 / 2.9	213	V14G (GTT→GGT)	xanP	xanthine permease
Reads supporting (aligned to +/- strand): ref base T (107/78); new base G (19/9); total (126/87)
Fisher's exact test for biased strand distribution p-value = 4.10e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 219 total aligned reads displayed.

AAAAAGTGCATTTATCTCATTTAAATATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCGGTGATCACGCCAGCGCTATTAATCTGCCAGGCGCTGGGTTTAC  >  CP009273/3822196‑3822494

acacaGTGCATTTATCTCATTTAAATATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGt                                                                                                                                                       <  2:4490085/146‑1 (MQ=255)

  aaaGTGCATTTATCTCATTTAAATATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTg                                                                                                                                                     <  1:3616222/150‑1 (MQ=255)

  aaaGTGCATTTATCTCATTTAAATATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTg                                                                                                                                                     <  2:2705326/150‑1 (MQ=255)

   aaGTGCATTTATCTCATTTAAATATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTgc                                                                                                                                                    >  2:2201118/1‑150 (MQ=255)

     gTGCATTTATCTCATTTAAATATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCACCCGGTTgcgc                                                                                                                                                  >  2:1986582/1‑150 (MQ=255)

         aTTTATCTCATTTAAATATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCCGTTGCGCAGAc                                                                                                                                              >  2:389066/1‑150 (MQ=255)

          tttATCTCATTTAAATATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACt                                                                                                                                             <  2:688140/150‑1 (MQ=255)

            tATCTCATTTAAATATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCa                                                                                                                                           >  2:1015936/1‑150 (MQ=255)

             aTCTCATTTAAATATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCCGTTGCGCACACTCaa                                                                                                                                          >  1:139228/1‑150 (MQ=255)

             aTCTCATTTAAATATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCCGGTGAGCAGACTCaa                                                                                                                                          >  1:2406059/1‑150 (MQ=255)

             aTCTCATTTAAATATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGACAAACCGGTTTCGCAGACTaaa                                                                                                                                          >  2:2919523/1‑150 (MQ=255)

              tctcATTTAAATATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGGTGCGCAGACTCaaa                                                                                                                                         <  2:1466969/150‑1 (MQ=255)

                  aTTTAAATATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCCACCCGTTGCGCACACACAAAAAAg                                                                                                                                     >  1:2963175/1‑150 (MQ=255)

                   tttAAATATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCCGGTGCGCAGACTCAAAAAAga                                                                                                                                    >  1:3914957/1‑149 (MQ=255)

                       attatTTAGATGTGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTGTGCTACCTCGGTATTGTTTTCGTTGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAAc                                                                                                                                <  2:2967088/148‑1 (MQ=255)

                       aaTATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGATTTGCTACCAGGGGTTTTTTTTCGATGTCTGTTTCCACCCTCGAGTCTGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAAc                                                                                                                                <  2:351709/150‑1 (MQ=255)

                          atTTTGCTGTGGTTGTGGCATTGGTTTGCTTTTCTCTTTGGGTCAGATAACCTGCCGGTTTTGCTACCTGGGTTTTTTTTTCGTTGTCTGTTTCCCCCCTGGAGTAAGAAAAGGCGCACCGGGTTGCGCAGACTCAAAACAGCGAACTGa                                                                                                                             <  1:2779557/150‑1 (MQ=255)

                             tAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCCCCCCCTTGCGCACACACAAAACAGCCAACAGAttt                                                                                                                          >  1:1027303/1‑150 (MQ=255)

                             tAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCCGTTGCGCAGACTCAAAACAACGAACAGAttt                                                                                                                          >  1:3730493/1‑150 (MQ=255)

                              aGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACAGATTTa                                                                                                                         >  2:4470651/1‑150 (MQ=255)

                                aTGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCCGGTGCGCACAAACAAAACACCGAACAGAttttaa                                                                                                                       >  1:40158/1‑147 (MQ=255)

                                 tGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAAAGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCg                                                                                                                      >  2:1057359/1‑150 (MQ=255)

                                  ttggATGTGGAAATCTTTTGCTTTTATCTTTCTGTCAGCTAAAATGCCCGTTTTGCTACCTGGGTTTTTTTTTCTTTGTTTTTTTCCACCCTCGAGACACAAAATGCCAAAGGTGTTGCCCAGACTCAAAACAGCGAACTGATTTACCGt                                                                                                                     <  1:23976/148‑1 (MQ=255)

                                             aaaCGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCg                                                                                                          <  2:1752379/150‑1 (MQ=255)

                                              aaCGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATAGt                                                                                                         >  2:1234498/1‑150 (MQ=255)

                                              aaCGTTTGCTTTTCTCTTTCGGTCAGCTAAAAAGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGt                                                                                                         >  2:2623325/1‑150 (MQ=255)

                                               tcGTTTGCTTTTCTCTTTCGTTCAGATAAAATGCCCGCTTTTCTACCAGGTGTTTTTTTTCTTTGTTTTTTACCACCCTCGAGTCATAAAATGCGCACCGGGTGGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTc                                                                                                        <  1:2177427/149‑1 (MQ=255)

                                                cGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCCACCGGTTGCGCAGACACAAAAAAGCGAACAGATTTACCGGCTTTAAAATCGTcc                                                                                                       >  1:1431784/1‑150 (MQ=255)

                                                cGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGtac                                                                                                       >  2:481880/1‑148 (MQ=255)

                                                cGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTcc                                                                                                       >  2:2929308/1‑150 (MQ=255)

                                                cGTTTGCTTTTCTCTTTCGGTCAGCTAAAAAGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCGTGAAGATCGTcc                                                                                                       >  2:3246385/1‑150 (MQ=255)

                                                      cTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCCCCCGGGTGCGCCGACTCAAAACAGCGAACTGGTTTAACGTCTTGTAGATCGTCcgccgca                                                                                                 >  2:2319741/1‑149 (MQ=255)

                                                        tttCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAAAAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTc                                                                                               >  2:2261340/1‑150 (MQ=255)

                                                          tctcTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCCACCGGTTGCGCAGACTCAAAACAGCGAACAGATTTACAGTCTTTAAGATCGTCcgccgcgtact                                                                                             >  1:3692771/1‑145 (MQ=255)

                                                          tctcTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCACCCCGTAGCGCACACACAAAACAGCGACCACAGATACCCACTTGAAGAACGACccccgcgacca                                                                                             >  1:2574338/1‑145 (MQ=255)

                                                          tctcTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACACAAAACAGCGAACTGATTTAACGTCTTGTATATAGTCCGCCGCTTcca                                                                                             >  1:2574681/1‑149 (MQ=255)

                                                          tctcTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGCGTGCGCAGCAACAAAAAAGCGAACTGATTTAACGTCTTGAAGATCATCCGCCGCATcca                                                                                             >  1:1810794/1‑149 (MQ=255)

                                                          tctcTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCCGTTGCGCAGACTCAAAACAACGACAAGATTTACCGTCTTTGAGATCGGCCGCCGCGTcca                                                                                             >  1:1409267/1‑149 (MQ=255)

                                                                tgggTCCGCTAAATGGCCGGCTTGGCTACCAGGGTTTTTTTTTCGTTGTGTGTGTCCCCCCCCGAGTCAGAAACGGCGCACGGGGGGGCCCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAAcc                                                                                       <  1:2324600/148‑1 (MQ=255)

                                                                tCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCCCCCTCGAGTCAGACAATGCGCCCCCGGTTGCGCCGACTCAAAACAGCGAACGGATTTACCGTCGTGTAGATCGTCCGCCGCGTCCGCCAAcc                                                                                       >  2:3782459/1‑150 (MQ=255)

                                                                tCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGGGCACCCGGTTTGGCCGACTCAAAAAAGCGAAATGATTTACCGGCTTGACTTTAGTCCGCCCCTGCCTCAAAcc                                                                                       >  1:3503596/1‑150 (MQ=255)

                                                                tCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAAAAACGAACAGATTTAACGTCTTGAAGATAGTCCGCCGCTTCCACAAAcc                                                                                       >  1:304023/1‑150 (MQ=255)

                                                                  ggTCTGCAAAAATGCCCGCTTTTCTACCACGGGATTGTGTTCGATGTCTTTTTCCACCCTCGAGTAAGAAAAGGCGCAACCGGTTGCGAAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCt                                                                                     <  2:3839413/150‑1 (MQ=255)

                                                                         tAAAAAGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGGTGCGCCGACTCAAAAAAGCGAACAGATTTAGCGTGTTGTAGATCGTCCGCCGCGTGCGCAAAACCAGtgtggc                                                                              >  1:3826262/1‑148 (MQ=255)

                                                                           aaaTGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCCACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTgccgc                                                                            >  2:284315/1‑150 (MQ=255)

                                                                            aaagCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCCACCGGTTGCGCAGACACAAAAAAGCGAAATGATTTACAGTCGTTAAGATCGTCCGCCGCGTCCTCAAAACCTGTTTgccgcc                                                                           >  1:691182/4‑150 (MQ=255)

                                                                            aaTGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTgccgcc                                                                           >  2:4288257/1‑150 (MQ=255)

                                                                                cccGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGGTGCGCAGACTCAAAAAAGCGAACAGATTTAACGTCTTGAAGATAGTCCACCGCATCCTCAAAACCAGTTTGCCGCCTGTc                                                                       >  1:851210/1‑150 (MQ=255)

                                                                                cccGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCCCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCACAAAACCTGTTTGCCCCCAGTc                                                                       >  2:4361807/1‑150 (MQ=255)

                                                                                 cctcTTTGCTACCAGTGTTTTGTTTTCGTTGTCTTTCACCCCCCTCTAGACACAAAAGGCGCAACCGGTTGCCCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCa                                                                      <  1:4203238/147‑1 (MQ=255)

                                                                                  ggCTTTGCTACCTGGGGTTTGTTTTGGTTGTGTGTTACCACCCTCGAGTCAGAAAATGCGCACCGGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAg                                                                     <  1:1659479/149‑1 (MQ=255)

                                                                                     tttGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCCACCCGGTGCGCAGACACAAAACACCGAACAGATTTACCCTCATGAAGATCGTCCGCCCCGTCCACAAACCCGGTTTGCCGCCAGTCCGCAt                                                                  >  2:421890/1‑150 (MQ=255)

                                                                                       tGCTACCACGGTATTGTTTTCGATGTCTGTTTCCACCCTCGAGAAATAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATct                                                                <  2:3472043/150‑1 (MQ=255)

                                                                                       tGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCCGAAAATGCGCAACCGGTTGCGCAGACTCAAAACATCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATca                                                                >  2:3723263/1‑149 (MQ=255)

                                                                                       tGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATct                                                                <  2:921100/150‑1 (MQ=255)

                                                                                       tGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACGGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATct                                                                >  2:1845698/1‑150 (MQ=255)

                                                                                           aCCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCCACCGGTTGCGCAGACTCAAAAAAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCGCAAACCCCGGTTGCCGCCTGTCAGCAActgctg                                                            >  1:2484555/1‑150 (MQ=255)

                                                                                           aCCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACACAAAAAACCGAACAGATTTACAGTCTTGAAGATAGTACGCCGCTTACTCAAAACAAGGTTTCCGCCGGTCAGCAtcttctt                                                            >  1:1724210/1‑149 (MQ=255)

                                                                                            ccccGGGAGTGTTTTCGATTGCTGTTACCCCCCTCGAGTCAGAAATTGCGCACCGGGTGGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTgg                                                           <  1:3232439/147‑1 (MQ=255)

                                                                                              aCGGGATTGTTTTCGATGTCTGTTTCCCCCCTCGAGACAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCg                                                         <  2:1500374/150‑1 (MQ=255)

                                                                                                 gtaTTGTTTTCGATGTCTGTTTCCCCCCTCGAGACAGAAATGGCGCACCCGGTCGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCGTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATg                                                      <  1:381321/148‑1 (MQ=255)

                                                                                                 ggATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCACCCGGTTGCGCCCACTCAAAACAGCGAACAGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCAGTTTGCCGCCAGTCAGCAACAGCTGGCGATg                                                      >  1:3383512/1‑150 (MQ=255)

                                                                                                  gtttGTTTTCGTTGTCTGTTTCCCCCCTCGAGTCAGAAATGGCGCACCGGGTTGCCCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGt                                                     <  1:3699805/148‑1 (MQ=255)

                                                                                                  gATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCCACCGGTTGCGCAGACTCAAAAAAGCGAACTGATTTACCGTCTTGAAGATAGTCCGCCGCTTCCTCAAACACTGTTTGACGCCTGTCAGCATCTGCTGGCGATGt                                                     >  2:23159/1‑150 (MQ=255)

                                                                                                    tttttttCGTTGTTTGTGACCACCCTCTAGTCATAAAATGCGCAACGGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTc                                                   <  1:1980872/147‑1 (MQ=255)

                                                                                                     tGTTTTCTCTTTCTGTTCCCACCCGCGAGTCATAAAATGCGCACCGGGGTGCCCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCg                                                  <  1:813626/150‑1 (MQ=255)

                                                                                                      gTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGt                                                 >  2:2540681/1‑150 (MQ=255)

                                                                                                      gTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACACAAAACAGCGAACTGTTTTAAAGTCTTTAATATCGTCCGCCGCTTCCTCAAACCCAGTTTTGCGCCGGTCAGCATCTGCTGGCGATGTTCGt                                                 >  1:1098981/1‑150 (MQ=255)

                                                                                                       ttttCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGtt                                                >  2:1812046/1‑150 (MQ=255)

                                                                                                       ttttCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCGCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGtt                                                >  2:1810881/1‑150 (MQ=255)

                                                                                                        tttctttGTCTTTTCCCACCCTCGATTCATAAAATGCCCAACCGGTGGCCCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTg                                               <  1:307331/144‑1 (MQ=255)

                                                                                                        tttCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGTAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTg                                               >  2:4107913/1‑150 (MQ=255)

                                                                                                         ttCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCCACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCGGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGc                                              >  2:961269/1‑150 (MQ=255)

                                                                                                         ttCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCCACCGGTTGCGCAGACACAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCATCCTCAAACCCGGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGc                                              >  2:808429/1‑150 (MQ=255)

                                                                                                             ttGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCGGTg                                          <  2:1850881/149‑1 (MQ=255)

                                                                                                              tGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCGGTGa                                         >  2:2726996/1‑150 (MQ=255)

                                                                                                              tGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCGGTGa                                         <  2:259257/150‑1 (MQ=255)

                                                                                                              tGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCAGTCAGCATCTGCTGGCGATGTTCGTTGCGGTGa                                         >  2:3954676/1‑150 (MQ=255)

                                                                                                                  tGTTCCCCCCCTCGAGTCAGAAACTGCGCACCGGGGGGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCGGTGATCAc                                     <  1:2939514/150‑1 (MQ=255)

                                                                                                                     ttCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCGGTGATCACGcc                                  >  2:3314270/1‑150 (MQ=255)

                                                                                                                     ttCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACACAAAACAGCGAACTGATTTACCGTCTTGAAGATAGTCCGCCGCGTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGAAGTTCGTTGCGGTGATCACGcc                                  >  2:3209740/1‑150 (MQ=255)

                                                                                                                     ttCCACCCTCGAGACAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCGGTGATCACGcc                                  <  2:3569270/150‑1 (MQ=255)

                                                                                                                      tCCACCCGCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCGGTGATCACGCCa                                 <  2:3392811/150‑1 (MQ=255)

                                                                                                                      accACCCTCGAGTCATAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCGGTGATCACGCCa                                 <  2:4114413/149‑1 (MQ=255)

                                                                                                                         aCCCGCGAGTCAGAAAATGCGCAACCTGTGGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCGGTGATCACGCCAgcg                              <  1:653781/150‑1 (MQ=255)

                                                                                                                            cTCGAGTCAGAAAATGCGCAAACGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTTAAGATCGTCCGCCCCTTCCTCAAACCCTGGTTTGCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCGGTGATCACGCCAGCGCtc                           >  2:4161912/1‑149 (MQ=255)

                                                                                                                                  tCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATCTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCGGTGATCACGCCAGCGCTATTAATc                     <  2:2778126/150‑1 (MQ=255)

                                                                                                                                  tCAGAAAAGGCGCACCCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCGGTGATCACGCCAGCGCTATTAATc                     <  2:3154749/150‑1 (MQ=255)

                                                                                                                                   cAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCACACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCGGTGATCACGCCAGCGCTATTAATCt                    <  2:1599204/150‑1 (MQ=255)

                                                                                                                                   actaaatTGCGCAACGGGTGGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCGGTGATCACGCCAGCGCTATTAATCt                    <  1:4379187/147‑1 (MQ=255)

                                                                                                                                    aGAAAATGCGCAACGGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCGGTGATCACGCCAGCGCTATTAATCTg                   <  2:1340241/150‑1 (MQ=255)

                                                                                                                                    aGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCGGTGATCACGCCAGCGCTATTAATCTg                   <  2:1039938/150‑1 (MQ=255)

                                                                                                                                    aGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCGGTGATCACGCCAGCGCTATTAATCTg                   <  2:2995722/150‑1 (MQ=255)

                                                                                                                                       ataggcgcAACGGGGGCCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCGGTGATCACGCCAGCGCTATTAATCTGCCa                <  1:1634254/146‑1 (MQ=255)

                                                                                                                                        aaTGCGCAACCGGTTGCGCAGACTCAAAACAGTGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCGGTGATCACGCCAGCGCTATTAATCTGCCAg               >  2:2383944/1‑150 (MQ=255)

                                                                                                                                              cAACCGGGTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCGGTGATCACGCCAGCGCTATTAATCTGCCAGGCGCTg         <  1:3133497/150‑1 (MQ=255)

                                                                                                                                               aaCCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCGGTGATCACGCCAGCGCTATTAATCTGCCAGGCGCTgg        <  1:3390663/150‑1 (MQ=255)

                                                                                                                                                 ccGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCGGTGATCACGCCAGCGCTATTAATCTGCCAGGCGCTGGGt      <  2:234257/150‑1 (MQ=255)

                                                                                                                                                   ggTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCGGTGATCACGCCAGCGCTATTAATCTGCCAGGCGCTGGGttt    >  1:828082/1‑150 (MQ=255)

                                                                                                                                                    gTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCGGTGATCACGCCAGCGCTATTAATCTGCCAGGCGCTGGGTTTa   <  1:1298275/150‑1 (MQ=255)

AAAAAGTGCATTTATCTCATTTAAATATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCGGTGATCACGCCAGCGCTATTAATCTGCCAGGCGCTGGGTTTAC  >  CP009273/3822196‑3822494

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`