BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	3,860,159	C→A	87.9%	P33P (CCG→CCT)	ibpB ←	heat shock chaperone

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	3,860,159	0	C	A	87.9%	458.4 / 67.3	221	P33P (CCG→CCT)	ibpB	heat shock chaperone
Reads supporting (aligned to +/- strand): ref base C (13/13); new base A (86/102); total (106/115)
Fisher's exact test for biased strand distribution p-value = 8.34e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.69e-01

Only 100 of 228 total aligned reads displayed.

TTTTTTCTCTTCTTTTGGCTGCTCCGGCGTGCCTTTTACGCTCAGGCGCGTACCTTCCAGTTGAATCTCTAAATCTTCCTGACGGAAACCTGCCAGCGCAAGGGTAATGCGGTAGTGGTTATCGTCGCTTTTCTCAATGTTGTACGGCGGGAAGCTCTGGCTTTCACCGGCGTTTTGCAGTGCGTTGGCCAGTTTGTCAAAACCGATCCATTGACGCATCAGTGGGGATAAATCGAAGTTACGCATAGTCATTTCTCCTTCTAAGAAGCGAGTAAGTACCTGCAAATCCGAAGATTC  >  CP009273/3860012‑3860308

ttttttCTCTTCTTTTGGCTGCTCCGGCGTGCCTTTTACGCTCAGGCGCGTACCTTCCAGTTGAATCTCTAAATCTTCCTGACGGAAACCTGCCAGCGCAAGGGTAATGCGGTAGTGGTTATCGTCGCTTTTCTCAATGTTGTAcggagg                                                                                                                                                     <  2:2538672/150‑1 (MQ=255)

 tttttCTCTTCTTTTGGCTGCTCCGGCGTGCCTTTTACGCTCAGGCGCGTACCTTCCAGTTGAATCTCTAAATCTTCCTGACGGAAACCTGCCAGCGCAAGGGTAATGCGGTAGTGGTTATCGTCGCTTTTATCAATTTTGTAAGGAggg                                                                                                                                                    >  1:1332086/1‑150 (MQ=255)

  ttttCTCTTCTTTTGGCTGCTCCGGCGTGCCTTTTACGCTCAGGCGCGTACCTTCCAGTTGAATCTCTAAATCTTCCTGACGGAAACCTGCCAGCGCAAGGGTAATGCGGTAGTGGTTATCGTCGCTTTTCTCAATGTTGTACGGCGGGa                                                                                                                                                   <  1:2536073/150‑1 (MQ=255)

   tttCTCTTCTTTTGGCTGCTCCGGCGTGCCTTTTACGCTCAGGCGCGTACCTTCCAGTTGAATCTCTAAATCTTCCTGACGGAAACCTGCCCGCGCAAGGGTAATGCGGTAGTGGTTATCGTCGCTTTTCTCAATGTTGTACGGAGGGaa                                                                                                                                                  <  2:1765989/150‑1 (MQ=255)

     tctcTTCTTTTGGCTGCTCCGGCGTGCCTTTTACGCTCAGGCGCGTACCTTCCAGTTGAATCTCTAAATCTTCCTGACGGAAACCTGCCAGCGCAAGGGTAATGCGGTAGTGGTTATCGTCGCTTTTCTCAATGTTGTACGGAGGGAAGc                                                                                                                                                <  1:3378869/150‑1 (MQ=255)

      ctctTCTTTTGGCTGCTCCGGCGTGCCTTTTACGCTCAGGCGCGTACCTTCCAGTTGAATCTCTAAATCTTCCTGACGGAAACCTGCCAGCGCAAGGGTAATGCGGTAGTGGTTATCGTCGCTTTTCTAAATGTTGTAAGGAGGGAAGct                                                                                                                                               >  1:3301899/1‑150 (MQ=255)

       tcttctTTTGGCTGCTCCGGCGTGCCTTTTACGCTCAGGCGCGTACCTTCCAGTTGAATCTCTAAATCTTCCTGACGGAAACCTGCCAGCGCAAGGGTAATGCGGTAGTGGTTATCGTCGCTTTTCTCAATTTTGTAAGGAGGGTAGcgc                                                                                                                                              >  1:3948440/1‑148 (MQ=255)

       tcttctTTTGGCTGCTCCGGCGTGCCTTTTACGCTCAGGCGCGTACCTTCCAGTTGAATCTCTAAATCTTCCTGACGGAAACCTGCCAGCGCAAGGGTAATGCGGTAGTGGTTATCGTCGCTTTTCTCAATGTTGTACGGAGGGAAGctc                                                                                                                                              <  1:3023049/150‑1 (MQ=255)

       tcttctTTTGGCTGCTCCGGCGTGCCTTTTACGCTCAGGCGCGTACCTTCCAGTTGAATCTCTAAATCTTCCTGACGGAAACCTGCCAGCGCAAGGGTAATGCGGTAGTGGTTATCGTCGCGTTTATAAATTTTTTTCGGAGGGAAGctc                                                                                                                                              >  1:531371/1‑150 (MQ=255)

           cttTTGGCTGCTCCGGCGTGCCTTTTACGCTCAGGCGCGTACCTTCCAGTTGAATCTCTAAATCTTCCTGACGGAAACCTGCCAGCGCAAGGGTAATGCGGTAGTGGTTATCGTCGCTTTTGTCAATGTTTTTAGGAGGGACGCGCTggg                                                                                                                                          >  1:2075013/1‑149 (MQ=255)

            ttttGGCTGCTCCGGCGTGCCTTTTACGCTCAGGCGCGTACCTTCCAGTTGAATCTCTAAATCTTCCTGACGGAAACCTGCCAGCGCAAGGGTAATGCGGTAGTGGTTATCGTCGCTTTTCTCAATGTTGTACGGAGGGAAGCTCTGGCt                                                                                                                                         <  2:4472079/150‑1 (MQ=255)

            ttttGGCTGCTCCGGCGTGCCTTTTACGCTCAGGCGCGTACCTTCCAGTTGAATCTCTAAATCTTCCTGACGGAAACCTGCCAGCGCAAGGGTAATGCGGTAGTGGTTATCGTCGCTTTTCTAAATGTTGTAAGGAGGGAAGCTCTGGCt                                                                                                                                         >  2:457370/1‑150 (MQ=255)

              ttGGCTGCTCCGGCGTGCCTTTTACGCTCAGGCGCGTACCTTCCAGTTGAATCTCTAAATCTTCCTGACGGAAACCTGCCAGCGCAAGGGTAATGCGGTAGTGGTTATCGTCGCTTTTCTCAATGTTGTACGGAGGGAAGCTCTGGCttt                                                                                                                                       <  1:4506680/150‑1 (MQ=255)

              ttGGCTGCTCCGGCGTGCCTTTTACGCTCAGGCGCGTACCTTCCAGTTGAATCTCTAAATCTTCCTGACGGAAACCTGCCAGCGCAAGGGTAATGCGGTAGTGGTTATCGTCGCTTTTCTCAATGTTGTACGGAGGGAAGCTCTGGCttt                                                                                                                                       <  1:3136775/150‑1 (MQ=255)

               tGGCTGCTCCGGCGTGCCTTTTACGCTCAGGCGCGTACCTTCCAGTTGAATCTCTAAATCTTCCTGACGGAAACCTGCCAGCGCAAGGGTAATGCGGTAGTGGTTATCGTCGCTTTTCTCAATGTTGTACGGAGGGAAGCTCTGGCttta                                                                                                                                      >  2:4325236/1‑149 (MQ=255)

                   tgctCCGGCGTGCCTTTTACGCTCAGGCGCGTACCTTCCAGTTGAATCTCTAAATCTTCCTGACGGAAACCTGCCAGCGCAAGGGTAATGCGGTAGTGGTTATCGTCGCTTTTATCCATGTTGTAAGGAGGGAAGCTCTGGCTTGAACCg                                                                                                                                  >  1:2793364/1‑150 (MQ=255)

                     ctCCGGCGTGCCTTTTACGCTCAGGCGCGTACCTTCCAGTTGAATCTCTAAATCTTCCTGACGGAAACCTGCCAGCGCAAGGGTAATGCGGTAGTGGTTATCGTCGCTTTTCTCAAAGGTGTTCGGATGGAAGCACTTGCGTTCACCGGc                                                                                                                                >  2:1130208/1‑150 (MQ=255)

                        cGGCGTGCCTTTTACGCTCAGGCGCGTACCTTCCAGTTGAATCTCTAAATCTTCCTGACGGAAACCTGCCAGCGCAAGGGTAATGCGGTAGTGGTTATCGTCGCTTTTCTCAATGTTGTACGGAGGGAAGCTCTGGCTTTCACCGGCGtt                                                                                                                             <  1:2925839/150‑1 (MQ=255)

                        cGGCGTGCCTTTTACGCTCAGGCGCGTACCTTCCAGTTGAATCTCTAAATCTTCCTGACGGAAACCTGCCAGCGCAAGGGTAATGCGGTAGTGGTTATCGTCGCTTTTCTCAATGTTGTACGGAGGGAAGCTCTGGCTTTCACCGGCGtt                                                                                                                             <  2:59613/150‑1 (MQ=255)

                         ggCGTGCCTTTTACGCTCAGGCGCGTACCTTCCAGTTGAATCTCTAAATCTTCCTGACGGAAACCTGCCAGCGCAAGGGTAATGCGGTAGTGGTTATCGTCGCTTTTCTCAAATTTGTTAGGTGGGAAGCTCTGGCTTTAAACGGCGgtt                                                                                                                            >  2:1194142/1‑150 (MQ=255)

                           cGTGCCTTTTACGCTCAGGCGCGTACCTTCCAGTTGAATCTCTAAATCTTCCTGACGGAAACCTGCCAGCGCAAGGGTAATGCGGTAGTGGTTATCGTCGCTTTTCTCAATGTTGTACGGAGGGAAGCTCTGGCTTTCACCGGCGTTTTg                                                                                                                          <  2:3626330/150‑1 (MQ=255)

                             tGCCTTTTACGCTCAGGCGCGTACCTTCCAGTTGAATCTCTAAATCTTCCTGACGGAAACCTGCCAGCGCAAGGGTAATGCGGTAGTGGTTATCGTCGCTTTTCTCAATGTTGTACGGAGGGAAGCTCTGGCTTTCACCGGCGGTTTGCa                                                                                                                        >  2:2933446/1‑150 (MQ=255)

                             tGCCTTTGACGCTCAGGCGCGGACCTTCCAGTTGAATCTCTAAATCTTCCTGACGGAAACCTGCCAGCGCAAGGGTAATGCGGTAGTGGTTATCGTCGCTTTTCTCAATGTTGTACGGAGGGAAGCTCTGGCTTTCACCGGCGTTTTGCa                                                                                                                        <  2:3980324/150‑1 (MQ=255)

                              gCCTTTTACGCTCAGGCGCGTACCTTCCAGTTGAATCTCTAAATCTTCCTGACGGAAACCTGCCAGCGCAAGGGTAATGCGGTAGTGGTTATCGTCGCTTTTATCAAAGTTTTACGGAGGGAAGCTCTGGCTTTCACCGGCGTTTTTCAg                                                                                                                       >  2:1646112/1‑150 (MQ=255)

                               ccTTTTACGCTCAGGCGCGTACCTTCCAGTTGAATCTCTAAATCTTCCTGACGGAAACCTGCCAGCGCAAGGGTAATGCGGTAGTGGTTATCGTCGCTTTTCTCAAAGTTGTAAGGAGGGAAGCTCTGGCTTTAAACGGCGGTTTGCAGt                                                                                                                      >  2:2761561/1‑150 (MQ=255)

                                cTTTTACGCTCAGGCGCGTACCTTCCAGTTGAATCTCTAAATCTTCCTGACGGAAACCTGCCAGCGCAAGGGTAATGCGGTAGTGGTTATCGTCGCTTTTCTCAATGTTGTACGGAGGGAAGCTCTGGCTTTCACCGGCGTTTTTCAGTg                                                                                                                     >  2:4253653/1‑150 (MQ=255)

                                    tACGCTCAGGCGCGTACCTTCCAGTTGAATCTCTAAATCTTCCTGACGGAAACCTGCCAGCGCAAGGGTAATGCGGTAGTGGTTATCGTCGCTTTTCTCAATGTTGTACGGAGGGAAGCTCTGGCTTTCACCGGCGTTTTGCAGTGCGtt                                                                                                                 <  2:2936896/150‑1 (MQ=255)

                                       gCTCAGGCGCGTACCTTCCAGTTGAATCTCTAAATCTTCCTGACGGAAACCTGCCAGCGCAAGGGTAATGCGGTAGTGGTTATCGTCGCTTTTCTCAATGTTGTACGGAGGGAAGCTCTGGCTTTCACCGGCGTTTTGCAGTGCGTTGGc                                                                                                              <  1:1338460/150‑1 (MQ=255)

                                        cTCAGGCGCGTACCTTCCAGTTGAATCTCTAAATCTTCCTGACGGAAACCTGCCAGCGCAAGGGTAATGCGGTAGTGGTTATCGTCGCTTTTCTCAATGTTGTACGGCGGGAAGCTCTGGCTTTCACCGGCGTTTTGCAGTGCGTTGGcc                                                                                                             <  2:967736/150‑1 (MQ=255)

                                         tCAGGCGCGTACCTTCCAGTTGAATCTCTAAATCTTCCTGACGGAAACCTGCCAGCGCAAGGGTAATGCGGTAGTGGTTATCGTCGCGTTTCTCAATGTTGTTCGGAGGGTAGCGCTGGGTTTAACCGGCGTTGTGAAGGGCGTTGGCCa                                                                                                            >  2:2152757/1‑150 (MQ=255)

                                           aGGCGCGTACCTTCCAGTTGAATCTCTAAATCTTCCTGACGGAAACCTGCCAGCGCAAGGGTAATGCGGTAGTGGTTATCGTCGCTTTTGTAAATTTTTTACGGAGGGAAGCTCTGGCGTTCACCGGCGGGTTTCACTGCGTTGGccccg                                                                                                          >  1:2462861/1‑147 (MQ=255)

                                           aGGCGCGTACCTTCCAGTTGAATCTCTAAATCTTCCTGACGGAAACCTGCCAGCGCAAGGGTAATGCGGTAGTGGTTATCGTCGCTTTTCTCAATGTTGTACGGAGGGAAGCTCTGGCTTTCACCGGCGTTTTGCAGTGCGTTGGCCAGt                                                                                                          <  1:4011388/150‑1 (MQ=255)

                                           aGGCGCGTACCTTCCAGTTGAATCTCTAAATCTTCCTGACGGAAACCTGCCAGCGCAAGGGTAATGCGGTAGTGGTTATCGTCGCTTTTCTCAATGTTGTACGGAGGGAAGCTCTGGCTTTCACCGGCGTTTTGCAGTGCGTTGGCCAGt                                                                                                          <  1:3663273/150‑1 (MQ=255)

                                            ggCGCGTACCTTCCAGTTGAATCTCTAAATCTTCCTGACGGAAACCTGCCAGCGCAAGGGTAATGCGGTAGTGGTTATCGTCGCGTTTCTCAATGTTGTAAGGTGGGAAGCTCTGGGTTTGACCGGCGGTTTTCAGTGCGTTGGCCAGtt                                                                                                         >  1:2973206/1‑150 (MQ=255)

                                               gcgTACCTTCCAGTTGAATCTCTAAATCTTCCTGACGGAAACCTGCCAGCGCAAGGGTAATGCGGTAGTGGTTATCGTCGCTTTTCTCAATGTTGTACGGAGGGAAGCTCTGGCTTTCACCGGCGTTTTGCAGTGCGTTGGCCAGTTTGt                                                                                                      <  1:4144232/150‑1 (MQ=255)

                                                cgTACCTTCCAGTTGAATCTCTAAATCTTCCTGACGGAAACCTGCCAGCGCAAGGGTAATGCGGTAGTGGTTATCGTCGCTTTTCTCAATGTTGTACGGAGGGAAGCTCTGGCTTTCACCGGCGTTTTGCAGTGCGTTGGCCAGTTTGTc                                                                                                     <  1:3759790/150‑1 (MQ=255)

                                                cgTACCTTCCAGTTGAATCTCTAAATCTTCCTGACGGAAACCTGCCAGCGCAAGGGTAATGCGGTAGTGGTTATCGTCGCTTTTCTCAATGTTGTACGGAGGGAAGCTCTGGCTTTCACCGGCGTTTTGCAGTGCGTTGGCCAGTTTGTc                                                                                                     <  1:3595705/150‑1 (MQ=255)

                                                  tACCTTCCAGTTGAATCTCTAAATCTTCCTGACGGAAACCTGCCAGCGCAAGGGTAATGCGGTAGTGGTTATCGTCGCTTTTGTCAATGGTTTTCGGAGGGAACCGCTGGCTTTCACCCGCCGGTTGCAGTGCGGGTGGCAGTTTGTGaa                                                                                                   >  1:1312985/1‑150 (MQ=255)

                                                  tACCTTCCAGTTGAATCTCTAAATCTTCCTGACGGAAACCTGCCAGCGCAAGGGTAATGCGGTAGTGGTTATCGTCGCTTTTATCAATGTTGTAAGGAGGGAAGATCTGGCGTTAAACGGCGGTTTTCAGTGCGTTGGCCAGGTTGTCaa                                                                                                   >  2:644638/1‑150 (MQ=255)

                                                     cTTCCAGTTGAATCTCTAAATATTCCTGACGGAAACCTGCCAGCGCAAGGGTAATGCGGTAGTGGTTATCGTCGCTTTTCTCAATGTTTTACGGAGGGAAGCTCTGGCTTTCACCGGCGTTTTTCAGTGCGTTGGCCAGTTTGTAaaaaa                                                                                                >  2:1781238/1‑149 (MQ=255)

                                                      ttCCAGTTGAATCTCTAACTCTTCCTGACGGAAACCTGCCAGCGCAAGGGTAATGCGGTAGTGGTTATCGTCGCTTTTCTCAATGTTGTACGGAGGGAAGCTCTGGCTTTCACCGGCGTTTTGCAGTGCGTTGGCCAGTTTGTCAAAAcc                                                                                               <  2:1145427/150‑1 (MQ=255)

                                                       tCCAGTTGAATCTCTAAATCTTCCTGACGGAAACCTGCCAGCGCAAGGGTAATGCGGTAGTGGTTATCGTCGCTTTTCTCAATGGTGTACGGAGGGAAGCGCTGGCGTTAACCGGCGTTTTGCAGTGCGTTTGCCAGTTTGTCaaaaacc                                                                                              >  2:4291185/1‑147 (MQ=255)

                                                          aGTTGAATCTCTAAATCTTCCTGACGGAAACCTGCCAGCGCATGTGTAATGCGGTAGTGGTTATCGTCGCTTTTCTCAATGTTGTACGGCGGGAAGCTCTGGCTTTCACCGGCGTTTTGCAGTGCGTTGGCCAGTTTGTCAAACCCGATc                                                                                           <  1:983501/150‑1 (MQ=255)

                                                             tGAATCTCTAAATCTTCCTGACGGAAACCTGCCAGCGCAAGGGTAATGCGGTAGTGGTTATCGTCGCTTTTCTCAATGTTGTACGGAGGGAAGCTCTGGCTTTCACCGGCGTTTTGCAGTGCGTTGGCCAGTTTGTCAAAACCGATCCAt                                                                                        <  1:4460563/150‑1 (MQ=255)

                                                                aTCTCTAAATCTTCCTGACGGAAACCTGCCAGCGCAAGGGTAATGCGGTAGTGGTTATCGTCGCGTTTCTCAATGTTGTACGGAGGGGAGCTCTGGCTTTCAACGGCGGTTTTCAGTGCGGTTGCCACGTTGTCAAAACCCATACAATGa                                                                                     >  1:3746900/1‑150 (MQ=255)

                                                                 tctcTAAATCTTCCTGACGGAAACCTGCCAGCGCAAGGGTAATGCGGTAGTGGTTATCGTCGCTTTTCTCAATGTTGTACGGAGGGAAGCTCTGGCTTTCACCGGCGTTTTGCAGTGCGTTGGCCAGTTTGTCAAAACCGATCCATTGAc                                                                                    <  1:143742/150‑1 (MQ=255)

                                                                  ctctAAATCTTCCTGACGGAAACCTGCCAGCGCAAGGGTAATGCGGTAGTGGTTATCGTCGCTTTTCTCAATGTTGTACGGAGGGAAGCTCTGGCTTTCACCGGCGTTTTGCAGTGCGTTGGCCAGTTTGTCAAAACCGATCCATTGACg                                                                                   <  1:3921707/150‑1 (MQ=255)

                                                                  ctctAAATCTTCCTGACGGAAACCTGCCAGCGCAAGGGTAATGCGGTAGTGGTTATCGTCGCTTTTCTCAATGTTGTACGGAGGGAAGCTCTGGCTTTCACCGGCGTTTTGCAGTGCGTTGGCCAGTTTGTCAAAACCGATCCATTGACg                                                                                   <  1:331231/150‑1 (MQ=255)

                                                                    ctAAATCTTCCTGACGGAAACCTGCCAGCGCAAGGGTAATGCGGTAGTGGTTATCGTCGCTTTTATAAATGTTGTAAGGAGGGTAGGTCTGGCTTTGACCGGCGGTTTTGAGTGCGGTGGGCAGGGTGTGAAAAACCATACAttttcgcc                                                                                 >  1:601100/1‑144 (MQ=255)

                                                                       aaTCTTCCTGACGGAAACCTGCCAGCGCAAGGGTAATGCGGTAGTGGTTATCGTCGCTTTTCTCAATGTTGTACGGAGGGAAGCTCTGGCTTTCACCGGCGTTTTGCAGTGCGTTGGCCAGTTTGTCAAAACCGATCCATTGACGCATCa                                                                              <  1:1039953/150‑1 (MQ=255)

                                                                        aTCTTCCTGACGGAAACCTGCCAGCGCAAGGGTAATGCGGTAGTGGTTATCGTCGCTTTTCTCAATGTTGTACGGAGGGAAGCTCTGGCTTTAACCGGCGTTTTGCAGTGCGTTTGCCCGTTTGTCAAAACCGATCCAATGACGCATCAg                                                                             >  2:1023001/1‑150 (MQ=255)

                                                                          cTTCCTGACGGAAACCTGCCAGCGCAAGGGTAATGCGGTAGTGGTTATCGTCGCTTTTCTCAATGGTGTACGGAGGGAAGCTCTGGCTTTCACCGGCGTTTTGCAGTGCGTTGGCCAGTTTTTCAAAACCGATCCATTGACGCATCAGTg                                                                           >  2:2948912/1‑150 (MQ=255)

                                                                           ttCCTGACGGAAACCTGCCAGCGCAAGGGTAATGCGGTAGTGGTTATCGTCGCTTTTCTCCAAGTTTTAAGGCGGGCAGCTCAGGCTTTCACCCGCGGTTTGCAGTGCGGTGGGCAGGTTTTCAAAAACCAAACAAAGACGCCTCAGTgg                                                                          >  2:1196434/1‑150 (MQ=255)

                                                                            tCCTGACGGAAACCTGCCAGCGCAAGGGTAATGCGGTAGTGGTTATCGTCGCTTTTCTCAATGTTGTACGGAGGGAAGCTCTGGCTTTCACCGGCGTTTTGCAGTGCGTTGGCCAGTTTGTCAAAACCGATCCATTGACGCATCAGTggg                                                                         <  2:2379561/150‑1 (MQ=255)

                                                                             ccTGACGGAAACCTGCCAGCGCAAGGGTAATGCGGTAGTGGTTATCGTCGCTTTTCTCAATGTTGTACGGAGGGAAGCTCTGGCTTTCACCGGCGTTTTTCAGTGCGTTGGCCAGTTTGTCAAAACCGATACATTGACGCATCAGTgggg                                                                        >  2:3272127/1‑150 (MQ=255)

                                                                                 aCGGAAACCTGCCAGCGCAAGGGTAATGCGGTAGTGGTTATCGTCGCTTTTCTCAATGTTGTACGGCGGGAAGCTCTGGCTTTCACCGGCGTTTTGCAGTGCGTTGGCCAGTTTGTCAAAACCGATCCATTGACGCATCAGTGGGGATaa                                                                    <  2:2387746/150‑1 (MQ=255)

                                                                                 aCGGAAACCTGCCAGCGCAAGGGTAATGCGGTAGTGGTTATCGTCGCTTTTCTCAATGTTGTACGGAGGGAAGCTCTGGCTTTCACCGGCGTTTTGCAGTGCGTTGGCCAGTTTGTCAAAACCGATCCATTGACGCATCAGTGGGGATaa                                                                    <  2:1713894/150‑1 (MQ=255)

                                                                                  cGGAAACCTGCCAGCGCAAGGGTAATGCGGTAGTGGTTATCGTCGCGTTTCGGCATGTTGGACCGGGGGTAGCTCTGGCGTTCACCGGCGTTTTGCAGTGCGGTGGCCAGGTTGTCCAAAACGCTACCTTGGCGCAGCCggggggctcaa                                                                   >  2:3099325/1‑145 (MQ=255)

                                                                                   ggAAACCTGCCAGCGCAAGGGTAATGCGGTAGTGGTTATCGTCGCTTTTCTCAATGTTGTACGGTGGGAAGCTCTGGCTTTCACCGGCGTTTTGCAGTGCGGTGGCCAGTTTGTCAAAACCGATACATTGTCGCCACAGGGGGGATAAAt                                                                  >  2:4144109/1‑150 (MQ=255)

                                                                                    gAAACCTGCCAGCGCAAGGGTAATGCGGTAGTGGTTATCGTCGCTTTTCTCAATGTTGTACGGAGGGAAGCTCTGGCTTTCACCGGCGTTTTGCAGTGCGTTGGCCAGTTTGTCAAAACCGATCCATTGACGCATCAGTGGGGATAAATc                                                                 <  2:131381/150‑1 (MQ=255)

                                                                                    gAAACCTGCCAGCGCAAGGGTAATGCGGTAGTGGTTATCGTCGCTTTTCTCAATGTTGTACGGAGGGAAGCTCTGGCTTTCACCGGCGTTTTGCAGTGCGTTGGCCAGTTTGTCAAAACCGATCCATTGACGCATCAGTGGGGATAAATc                                                                 <  2:4178858/150‑1 (MQ=255)

                                                                                       aCCTGCCAGCGCAAGGGTAATGCGGTAGTGGTTATCGTCGCGTTTATCAAAGTTGTACGGAGGGAAGCACAGGCGGTCACCCGCGGTTTGCAGTGCGGTGGGCCCGTTGTCAAAACCCAACCATTGACGCCTCAGGGGGGGTAAATAGaa                                                              >  2:450428/1‑150 (MQ=255)

                                                                                         cTGCCAGCGCAAGGGTAATGCGGTAGTGGTTATCGTCGCTTTTCTCAATGTTGTACGGAGGGAAGCTCTGGCGTTCACCGGCGGTTTTCAGTTCGTTGGCCAGTTTTTCAAAAACGATCCAATGAAGCATCAGGGGGGTTAAATCGAAGt                                                            >  2:3021549/1‑150 (MQ=255)

                                                                                          tGCCAGCGCAAGGGTAATGCGGTAGTGGTTATCGTCGCTTTTCTCAATGTTGTACGGAGGGAAGCTCTGGCTTTCACCGGCGTTTTGCAGTGCGTTGGCCAGTTTGTCAAAACCGATCCATTGACGCATCAGTGGGGATAAATCGAAGtt                                                           <  2:4350877/150‑1 (MQ=255)

                                                                                              aGCGCAAGGGTAATGCGGTAGTGGTTATCGTCGCTTTTCTCAATGTTGTACGGAGGGAAGCTCTGGCTTTCACCGGCGTTTTTCAGTGCGGTGGCCAGGTTGTCAAAAACGATACATTGACGCATCCGTGGGGATAAATAGAAGTTAAGc                                                       >  2:3659681/1‑150 (MQ=255)

                                                                                              aGCGCAAGGGTAATGCGGTAGTGGTTATCGTCGCTTTTATCAATTTTTTAAGGAGGGAAGCTCTGGGTTTAACCGGCGTTTTTCAGTGCGTTTGCCAGTTTTTCAAAAACCATCCATTTACGCATCAGTGGGGGTAAATCGAAGTTAAGc                                                       >  2:2867846/1‑150 (MQ=255)

                                                                                                cgcAAGGGTAATGCGGTAGTGGTTATCGTCGCTTTTCTCAATGGTGTACGGAGGGAAGCTCTGGCTTTCACCGGCGTTTTGCAGTGCGTTGGCCAGTTTGTCAAAACCGATCCATTGACGCATCAGTGGGGATAAATCGAAGTTACGCAt                                                     <  2:2948299/150‑1 (MQ=255)

                                                                                                  cAAGGGTAATGCGGTAGTGGTTATCGTCGCTTTTCTCAATGTTGTACGGCGGGAAGCTCTGGCTTTCACCGGCGTTTTGCAGTGCGTTGGCCAGTTTGTCAAAACCGATCCATTGACGCATCAGTGGGGATAAATCGAAGTTACGCATAg                                                   <  1:1567799/150‑1 (MQ=255)

                                                                                                   aaGGGTAATGCGGTAGTGGTTATCGTCGCTTTTCTCAATGTTGTACGGAGGGAAGCTCTGGCTTTCACCGGCGTTTTGCAGTGCGTTGGCCAGTTTGTCAAAACCGATCCATTGACGCATCAGTGGGGATAAATCGAAGTTACGCATAGt                                                  <  1:207091/150‑1 (MQ=255)

                                                                                                      ggTAATGCGGTAGTGGTTATCGTCGCTTTTCTCAATTTTTTAAGGAGGGAAGCTCTGGCTTTCAACGGCGTTTTTCAGTGGGTTGGGCAGTTTGTCAAAAACGATCCATTGAAGCAAAAGTGGGGTTAAATAGAAGTTAAGCAAAGtaaa                                               >  2:1352767/1‑147 (MQ=255)

                                                                                                      ggTAATGCGGTAGTGGTTATCGTCGCTTTTCTCAATGTTGTACGGAGGGAAGCTCTGGCTTTCACCGGCGTTTTGCAGTGCGTTGGCCAGTTTGTCAAAACCGATCCATTGACGCATCAGTGGGGATAAATCGAAGTTACGCATAGTCAt                                               <  1:2479458/150‑1 (MQ=255)

                                                                                                      ggTAATGCGGTAGTGGTTATCGTCGCTTTTATAAATGTTTTACCGAGGGAAGCTCTGGCTTTCACCCGCGGTTTGCCGTGCGTTGGCCCGTTTGTGAAAAACGATCCAATTACGCATCAGTGGGGATAAAACGAAGTTTAGCAAAGTAAt                                               >  2:3342195/1‑150 (MQ=255)

                                                                                                       gTAATGCGGTAGTGGTTATCGTCGCTTTTCTCAATGTTGTACGGAGGGAAGCTCAGGCGTTCACCGGCGTTTTGCAGTGCCTTGGCCCGTTTGTCAAAACCGATCCCTTGACGCCTCACTGGGGATAAAACGAAGTTACCCAAAGTCaat                                              >  2:2219274/1‑148 (MQ=255)

                                                                                                        tAATGCGGTAGTGGTTATCGTCGCTTTTCTCAATGTTGTACGGAGGGAAGCTCTGGCTTTCACCGGCGTTTTGCAGTGCGTTGGCCAGTTTGTCAAAACCGATCCATTGACGCATCAGTGGGGATAAATCGAAGTTACGCATAGTCAttt                                             <  2:4231378/150‑1 (MQ=255)

                                                                                                        tAATGCGGTAGTGGTTATCGTCGCTTTTCTCAATGTTGTACGGAGGGAAGCTCTGGCTTTCACCGGCGTTTTGCAGTGCGTTGGCCAGTTTGTCAAAACCGATCCATTGACGCATCAGTGGGGATAAATCGAAGTTACGCATAGTCAttt                                             <  1:1194131/150‑1 (MQ=255)

                                                                                                         aaTGCGGTAGTGGTTATCGTCGCTTTTCTCAATGTTGTACGGAGGGAAGCTCTGGCTTTCACCGGCGTTTTGCAGTGCGTTGGCCAGTTTGTCAAAACCGATCCATTGACGCATCAGTGGGGATAAATCGAAGTTACGCATAGTCATTtc                                            <  2:1839098/150‑1 (MQ=255)

                                                                                                            gCGGTAGTGGTTATCGTCGCTTTTCTCAATGTTGTACGGAGGGAAGCTCTGGCTTTCACCGGCGTTTTGCAGTGCGTTGGCCAGTTTGTCAAAACCGATCCATTGACGCATCAGTGGGGATAAATCGAAGTTACGCATAGTCATTTCTcc                                         <  2:4152852/150‑1 (MQ=255)

                                                                                                              ggTAGTGGTTATCGTCGCTTTTCTCAATGTTGTACGGAGGGAAGCTCTGGCTTTCACCGGCGTTTTGCAGTGCGTTGGCCAGTTTGTCAAAACCGATCCATTGACGCATCAGTGGGGATAAATCGAAGTTACGCATAGTCATTTCTCCtt                                       <  2:2441095/150‑1 (MQ=255)

                                                                                                                  gTGGTTATCGTCGCTTTTCTCAATGTTGTACGGAGGGAAGCTCTGGCTTTCACCGGCGTTTTGCAGTGCGTTGGCCAGTTTGTCAAAACCGATCCATTGACGCATCAGTGGGGATAAATCGAAGTTACGCATAGTCATTTCTCCTTCTaa                                   <  1:3988925/150‑1 (MQ=255)

                                                                                                                  gTGGTTATCGTCGCTTTTCTAAATTTTGTAAGGAGGGAATCTCTGGCTTTAACCGGCGTTTTGCAGTGCGTTTGCCAGTTTTTAAAAAACGATCCATTTACCCATCAGTGGGGAAAAAAAGAAGTTACGCATAGTCATTTATCCTTCTaa                                   >  2:2568138/1‑150 (MQ=255)

                                                                                                                     gTTATCGTCGCTTTTCTCAATGTTGTACGGAGGGAAGCTCTGGCTTTCACCGGCGTTTTGCAGTGCGTTGGCCAGTTTGTCAAAACCGATCCATTGACGCATCAGTGGGGATAAATCGAAGTTACGCATAGTCATTTCTCCTTCTaagaa                                <  1:4157822/150‑1 (MQ=255)

                                                                                                                          cgtcgCTTTTCTCAATGTTGTACGGAGGGAAGCTCTGGCTTTCACCGGCGTTTTGCAGTGCGTTGGCCAGTTTGTCAAAACCGATCCATTGACGCATCAGTGGGGATAAATCGAAGTTACGCATAGTCATTTCTCCTTCTAAGAAGCGag                           >  2:1827914/1‑150 (MQ=255)

                                                                                                                          cgtcgCTTTTCTCAATGTTGTACGGAGGGAAGCTCTGGCTTTCACCGGCGTTTTGCAGTGCGTTGGCCAGTTTGTCAAAACCGATCCATTGACGCATCAGTGGGGATAAATCGAAGTTACGCATAGTCATTTCTCCTTCTAAGAAGCGag                           <  2:948537/150‑1 (MQ=255)

                                                                                                                          cgtcgCTTTTCTCAATGTTGTACGGAGGGAAGCTCTGGCTTTCACCGGCGTTTTGCAGTGCGTTGGCCAGTTTGTCAAAAACGATACATTTAAGCATCAGTGGGGATAAATAGAAGTTACCCAAACTCAATTATACATCTAAGAAGCGag                           >  2:3210757/1‑150 (MQ=255)

                                                                                                                           gtcgCTTTTCTCAATGTTGTACGGCGGGAAGCTCTGGCTTTCACCGGCGTTTTGCAGTGCGTTGGCCAGTTTGTCAAAACCGATCCATTGAAGCATCAGTGGGGATAAATAGAAGTTAAGCATAGTCATTTATCCTTCTAATAAGCGagt                          >  2:844848/1‑150 (MQ=255)

                                                                                                                           gtcgCTTTTCTCAATGTTGTACGGAGGGAAGCTCTGGCTTTCACCGGCGTTTTGCAGTGCGTTGGCCAGTTTGTCAAAACCGATCCATTGACGCATCAGTGGGGATAAATCGAAGTTACGCATAGTCATTTCTCCTTCTAAGAAGCGagt                          >  2:3563650/1‑150 (MQ=255)

                                                                                                                              gCTTTTCTCAATGTTGTACGGCGGGAAGCTCTGGCTTTCACCGGCGTTTTGCAGTGCGTTGGCCAGTTTGTCAAAACCGATCCATTGACGCATCAGTGGGGATAAATCGAAGTTACGCATAGTCATTTCTCATTCTAAGAATCGagtaag                       >  2:3688466/1‑150 (MQ=255)

                                                                                                                              gCTTTTCTCAATGTTGTACGGAGGGAAGCTCTGGCTTTCACCGGCGTTTTGCAGTGCGTTGGCCAGTTTGTCAAAACCGATCCATTGACGCATCAGTGGGGATAAATCGAAGTTACGCATAGTCATTTCTCCTTCTAAGAAGCGagtaag                       <  2:3437320/150‑1 (MQ=255)

                                                                                                                              gCTTTTCTCAATGTTGTACGGAGGGAAGCTCTGGCTTTCACCGGCGTTTTGCAGTGCGTTGGCCAGTTTGTCAAAACCGATCCATTGACGCATCAGTGGGGATAAATCGAAGTTACGCATAGTCATTTCTCCTTCTAAGAAGCGagtaag                       >  1:2570798/1‑150 (MQ=255)

                                                                                                                               cTTTTCTCAATGTTGTACGGCGGGAAGCTCTGGCTTTCACCGGCGTTTTGCAGTGCGTTGGCCAGTTTGTCAAAACCGATCCATTGACGCATCAGTGGGGATAAATCGAAGTTACGCATAGTCATTTCTCCTTCTAAGAAGCGagtaagt                      >  1:4159259/1‑150 (MQ=255)

                                                                                                                                  ttCTCAATGTTGTACGGAGGGAAGCTCTGGCTTTCACCGGCGTTTTGCAGTGCGTTGGCCAGTTTGTCAAAACCGATCCATTGACGCATCAGTGGGGATAAATCGAAGTTACGCATAGTCATTTCTCCTTCTAAGAAGCGAGTAAGTAcc                   >  1:1071875/1‑150 (MQ=255)

                                                                                                                                   tctcAATGTTGTACGGAGGGAAGCTCTGGCTTTCACCGGCGTTTTGCAGTGCGTTGGCCAGTTTGTCAAAACCGATCCATTGACGCATCAGTGGGGATAAATCGAAGTTACGCATAGTCATTTCTCCTTCTAAGAAGCGAGTAAGTACCt                  <  1:3282686/150‑1 (MQ=255)

                                                                                                                                   tctcAATGTTGTACGGAGGGAAGCTCTGGCTTTCACCGGCGTTTTGCAGTGCGTTGGCCAGTTTGTCAAAACCGATCCATTGACGCATCAGTGGGGATAAATCGAAGTTACGCATAGTCATTTCTCCTTCTAAGAAGCGAGTAAGTACCt                  <  2:263524/150‑1 (MQ=255)

                                                                                                                                   tctcAATGTTGTACGGAGGGAAGCTCTGGCTTTCACCGGCGTTTTGCAGTGCGTTGGCCAGTTTGTCAAAACCGATCCATTGACGCATCAGTGGGGATAAATCGAAGTTACGCATAGTCATTTCTCCTTCTAAGAAGCGAGTAAGTACCt                  <  1:1005113/150‑1 (MQ=255)

                                                                                                                                    ctcAATGTTGTACGGAGGGAAGCTCTGGCTTTCACCGGCGTTTTGCAGTGCGTTGGCCAGTTTGTCAAAACCGATCCATTGACGCATCAGTGGGGATAAATCGAAGTTACGCATAGTCATTTCTCCTTCTAAGAAGCGAGTAAGTACCTg                 <  1:3255858/150‑1 (MQ=255)

                                                                                                                                           ttgtACGGAGGGAAGCTCTGGCTTTCACCGGCGTTTTGCAGTGCGTTGGCCAGTTTGTCAAAACCGATCCATTGACGCATCAGTGGGGATAAATCGAAGTTACGCATAGTCATTTCTCCTTCTAAGAAGCGAGTAAGTACCTGCAAATcc          <  2:736178/150‑1 (MQ=255)

                                                                                                                                           ttgtACGGAGGGAAGCTCTGGCTTTCACCGGCGTTTTGCAGTGCGTTGGCCAGTTTGTCAAAACCGATCCATTGACGCATCAGTGGGGATAAATCGAAGTTACGCATAGTCATTTCTCCTTCTAAGAAGCGAGTAAGTACCTGCAAATcc          <  2:1029948/150‑1 (MQ=255)

                                                                                                                                           ttgtACGGAGGGAAGCTCTGGCTTTCACCGGCGTTTTGCAGTGCGTTGGCCAGTTTGTCAAAACCGATCCATTGACGCATCAGTGGGGATAAATCGAAGTTACGCATAGTCATTTCTCCTTCTAAGAAGCGAGTAAGTACCTGCAAATcc          <  2:1795888/150‑1 (MQ=255)

                                                                                                                                                cggcggGAAGCTCTGGCTTTCACCGGCGTTTTGCAGTGCGTTGGCCAGTTTGTCAAAACCGATCCATTGACGCATCAGTGGGGATAAATCGAAGTTACGCCTAGTCATTTCTCCTTCTAAGAAGCGAGTAAGTACCTGCAAATCCGAAGa     >  2:783187/1‑150 (MQ=255)

                                                                                                                                                cggaggGAAGCTCTGGCTTTCACCGGCGTTTTGCAGTGCGTTGGCCAGTTTGTCAAAACCGATCCATTGACGCATCAGTGGGGATAAATCGAAGTTACGCATAGTCATTTCTCCTTCTAAGAAGCGAGTAAGTACCTGCAAATCCGAAGa     >  2:3481171/1‑150 (MQ=255)

TTTTTTCTCTTCTTTTGGCTGCTCCGGCGTGCCTTTTACGCTCAGGCGCGTACCTTCCAGTTGAATCTCTAAATCTTCCTGACGGAAACCTGCCAGCGCAAGGGTAATGCGGTAGTGGTTATCGTCGCTTTTCTCAATGTTGTACGGCGGGAAGCTCTGGCTTTCACCGGCGTTTTGCAGTGCGTTGGCCAGTTTGTCAAAACCGATCCATTGACGCATCAGTGGGGATAAATCGAAGTTACGCATAGTCATTTCTCCTTCTAAGAAGCGAGTAAGTACCTGCAAATCCGAAGATTC  >  CP009273/3860012‑3860308

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`