Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,960,036 G→A 92.9% R87H (CGC→CAC)  rho → transcription termination factor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,960,0360GA92.9% 913.1 / 41.8 273R87H (CGC→CAC) rhotranscription termination factor
Reads supporting (aligned to +/- strand):  ref base G (10/9);  new base A (125/127);  total (135/138)
Fisher's exact test for biased strand distribution p-value = 8.17e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.67e-01

Only 100 of 276 total aligned reads displayed.

TCCTGAAGCAGCACGCAAAGAGTGGCGAAGATATCTTTGGTGATGGCGTACTGGAGATATTGCAGGATGGATTTGGTTTCCTCCGTTCCGCAGACAGCTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCCGCCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGAACGCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGCAACAAAATCCTCTTTGAGAACTTA  >  CP009273/3959889‑3960184
                                                                                                                                                   |                                                                                                                                                    
tCCTGAAGCAGCACGCAAAGAGTGGCGAAGATATCTTTGGTGATGGCGTACTGGAGATATTGCAGGATGGATTTGGTTTCCTCCGTTCCGCAGACAGCTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATccacc                                                                                                                                                    <  1:1205701/150‑1 (MQ=255)
   tGAAGCAGCACGCAAAGAGTGGCGAAGATATCTTTGGTGATGGCGTACTGGAGATATTGCAGGATGGATTTGGTTTCCTCCGTTCCGCAGACAGCTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCCACCGtt                                                                                                                                                 >  1:3809654/1‑150 (MQ=255)
   tGAAGCAGCACGCAAAGAGTGGCGAAGATATCTTTGGTGATGGCGTACTGGAGATATTGCAGGATGGATTTGGTTTCCTCCGTTCCGCAGACAGCTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCCACCGtt                                                                                                                                                 >  1:2042940/1‑150 (MQ=255)
    gAAGCAGCACGCAAAGAGTGGCGAAGATATCTTTGGTGATGGCGTACTGGAGATATTGCAGGATGGATTTGGTTTCCTCCGTTCCGCAGACAGCTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCCACCGttt                                                                                                                                                >  2:785563/1‑150 (MQ=255)
    gAAGCAGCACGCAAAGAGTGGCGAAGATATCTTTGGTGATGGCGTACTGGAGATATTGCAGGATGGATTTGGTTTCCTCCGTTCCGCAGACAGCTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCCACCGttt                                                                                                                                                >  1:1673278/1‑150 (MQ=255)
     aaGCAGCACGCAAAGAGTGGCGAAGATATCTTTGGTGATGGCGTACTGGAGATATTGCAGGATGGATTTGGTTTCCTCCGTTCCGCAGACAGCTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCCACCGTTTc                                                                                                                                               <  1:2362110/150‑1 (MQ=255)
         agcaCGCAAAGAGTGGCGAAGATATCTTTGGTGATGGCGTACTGGAGATATTGCAGGATGGATTTGGTTTCCTCCGTTCCGCAGACAGCTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCCACCGTTTCAAcc                                                                                                                                           >  2:1611759/1‑150 (MQ=255)
          gcaCGCAAAGAGTGGCGAAGATATCTTTGGTGATGGCGTACTGGAGATATTGCAGGATGGATTTGGTTTCCTCCGTTCCGCAGACAGCTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCCACCGTTTCAACCt                                                                                                                                          <  1:1036527/150‑1 (MQ=255)
              gCAAAGAGTGGCGAAGATATCTTTGGTGATGGCGTACTGGAGATATTGCAGGATGGATTTGGTTTCCTCCGTTCCGCAGACAGCTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCCACCGTTTCAACCTCCGc                                                                                                                                      >  1:1462846/1‑150 (MQ=255)
              gCAAAGAGTGGCGAAGATATCTTTGGTGATGGCGTACTGGAGATATTGCAGGATGGATTTGGTTTCCTCCGTTCCGCAGACAGCTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCCACCGTTTCAACCTCCGc                                                                                                                                      >  1:3982907/1‑150 (MQ=255)
               cAAAGAGTGGCGAAGATATCTTTGGTGATGGCGTACTGGAGATATTGCAGGATGGATTTGGTTTCCTCCGTTCCGCAGACAGCTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCCACCGTTTCAACCTCCGCa                                                                                                                                     >  2:2839190/1‑150 (MQ=255)
                 aaGAGTGGCGAAGATATCTTTGGTGATGGCGTACTGGAGATATTGCAGGATGGATTTGGTTTCCTCCGTTCCGCAGACAGCTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCCACCGTTTCAACCTCCGCACt                                                                                                                                   <  1:1070956/150‑1 (MQ=255)
                   gagTGGCGAAGATATCTTTGGTGATGGCGTACTGGAGATATTGCAGGATGGATTTGGTTTCCTCCGTTCCGCAGACAGCTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCCACCGTTTCAACCTCCGCACTgg                                                                                                                                 <  2:1470219/150‑1 (MQ=255)
                    agTGGCGAAGATATC‑TTGGTGATGGCGTACTGGAGATATTGCAGGATGGATTTGGTTTCCTCCGTTCCGCAGACAGCTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCCACCGTTTCAACCTCCGCACTGGTg                                                                                                                               >  1:1324580/1‑150 (MQ=255)
                     gTGGCGAAGATATCTTTGGTGATGGCGTACTGGAGATATTGCAGGATGGATTTGGTTTCCTCCGTTCCGCAGACAGCTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCCACCGTTTCAACCTCCGCACTGGTg                                                                                                                               >  1:599995/1‑150 (MQ=255)
                      tGGCGAAGATATCTTTGGTGATGGCGTACTGGAGATATTGCAGGATGGATTTGGTTTCCTCCGTTCCGCAGACAGCTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCCACCGTTTCAACCTCCGCACTGGTGa                                                                                                                              <  2:3627942/150‑1 (MQ=255)
                       ggCGAAGATATCTTTGGTGATGGCGTACTGGAGATATTGCAGGATGGATTTGGTTTCCTCCGTTCCGCAGACAGCTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCCACCGTTTCAACCTCCGCACTGGTGAt                                                                                                                             <  1:169764/150‑1 (MQ=255)
                        gCGAAGATATCTTTGGTGATGGCGTACTGGAGATATTGCAGGATGGATTTGGTTTCCTCCGTTCCGCAGACAGCTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCCACCGTTTCAACCTCCGCACTGGTGATa                                                                                                                            <  1:1501789/150‑1 (MQ=255)
                        gCGAAGATATCTTTGGTGATGGCGTACTGGAGATATTGCAGGATGGATTTGGTTTCCTCCGTTCCGCAGACAGCTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCCACCGTTTCAACCTCCGCACTGGTGATa                                                                                                                            <  1:2381/150‑1 (MQ=255)
                          gAAGATATCTTTGGTGATGGCGTACTGGAGATATTGCAGGATGGATTTGGTTTCCTCCGTTCCGCAGACAGCTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCCACCGTTTCAACCTCCGCACTGGTGATAcc                                                                                                                          <  2:3880919/150‑1 (MQ=255)
                            aGATATCTTTGGTGATGGCGTACTGGAGATATTGCAGGATGGATTTGGTTTCCTCCGTTCCGCAGACAGCTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCCACCGTTTCAACCTCCGCACTGGTGATTCCAt                                                                                                                        <  2:4235785/150‑1 (MQ=255)
                                atCTTTGGTGATGGCGTACTGGAGATATTGCAGGATGGATTTGGTTTCCTCCGTTCCGCAGACAGCTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCCACCGTTTCAACCTCCGCACTGGTGATACCATctct                                                                                                                    >  2:3957553/1‑150 (MQ=255)
                                atCTTTGGTGATGGCGTACTGGAGATATTGCAGGATGGATTTGGTTTCCTCCGTTCCGCAGACAGCTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCCACCGTTTCAACCTCCGCACTGGTGATACCATctct                                                                                                                    <  1:1268610/150‑1 (MQ=255)
                                  cTTTGGTGATGGCGTACTGGAGATATTGCAGGATGGATTTGGTTTCCTCCGTTCCGCAGACAGCTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCCGCCGTTTCAACCTCCGCACTGGTGATACCATCTCTgg                                                                                                                  <  1:2629517/150‑1 (MQ=255)
                                   tttGGTGATGGCGTACTGGAGATATTGCAGGATGGATTTGGTTTCCTCCGTTCCGCAGACAGCTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCCACCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGt                                                                                                                 >  1:1478670/1‑150 (MQ=255)
                                      ggTGATGGCGTACTGGAGATATTGCAGGATGGATTTGGTTTCCTCCGTTCCGCAGACAGCTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCCACCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAg                                                                                                              >  1:4383940/1‑150 (MQ=255)
                                       gTGATGGCGTACTGGAGATATTGCAGGATGGATTTGGTTTCCTCCGTTCCGCAGACAGCTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCCACCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGa                                                                                                             <  2:2484974/150‑1 (MQ=255)
                                       gTGATGGCGTACTGGAGATATTGCAGGATGGATTTGGTTTCCTCCGTTCCGCAGACAGCTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCCACCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGa                                                                                                             <  1:3962909/150‑1 (MQ=255)
                                        tGATGGCGTACTGGAGATATTGCAGGATGGATTTGGTTTCCTCCGTTCCGCAGACAGCTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCCACCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGAt                                                                                                            <  2:839506/150‑1 (MQ=255)
                                        tGATGGCGTACTGGAGATATTGCAGGATGGATTTGGTTTCCTCCGTTCCGCAGACAGCTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCCACCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGAt                                                                                                            >  2:33128/1‑150 (MQ=255)
                                        tGATGGCGTACTGGAGATATTGCAGGATGGATTTGGTTTCCTCCGTTCCGCAGACAGCTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCCACCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGAt                                                                                                            >  2:602866/1‑150 (MQ=255)
                                         gATGGCGTACTGGAGATATTGCAGGATGGATTTGGTTTCCTCCGTTCCGCAGACAGCTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCCACCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGAtt                                                                                                           <  2:146963/150‑1 (MQ=255)
                                         gATGGCGTACTGGAGATATTGCAGGATGGATTTGGTTTCCTCCGTTCCGCAGACAGCTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCCACCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGAtt                                                                                                           <  1:458712/150‑1 (MQ=255)
                                          aTGGCGTACTGGAGATATTGCAGGATGGATTTGGTTTCCTCCGTTCCGCAGACAGCTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCCACCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTc                                                                                                          >  1:1989443/1‑150 (MQ=255)
                                           tGGCGTACTGGAGATATTGCAGGATGGATTTGGTTTCCTCCGTTCCGCAGACAGCTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCCACCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTcg                                                                                                         >  1:696602/1‑150 (MQ=255)
                                           tGGCGTACTGGAGATATTGCAGGATGGATTTGGTTTCCTCCGTTCCGCAGACAGCTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCCACCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTcg                                                                                                         >  1:2954404/1‑150 (MQ=255)
                                              cGTACTGGATATATTGCAGGATGGATTTGGTTTCCTCCGTTCCGCAGACAGCTCCGACCTCGCCGGTCCTGATGACATCTACTTTTCCCCTAGCCAAATCCACCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTcgccc                                                                                                      <  2:1310408/150‑1 (MQ=255)
                                               gTACTGGAGATATTGCAGGATGGATTTGGTTTCCTCCGTTCCGCAGACAGCTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCCGCCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTcgcccg                                                                                                     <  2:887784/150‑1 (MQ=255)
                                               gTACTGGAGATATTGCAGGATGGATTTGGTTTCCTCCGTTCCGCAGACAGCTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCCACCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTcgcccg                                                                                                     >  1:3055271/1‑150 (MQ=255)
                                               gTACTGGAGATATTGCAGGATGGATTTGGTTTCCTCCGTTCCGCAGACAGCTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCCACCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTcgcccg                                                                                                     >  1:3563543/1‑150 (MQ=255)
                                                tACTGGAGATATTGCAGGATGGATTTGGTTTCCTCCGTTCCGCAGACAGCTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCCACCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTcgcccgc                                                                                                    >  2:2180180/1‑150 (MQ=255)
                                                tACTGGAGATATTGCAGGATGGATTTGGTTTCCTCCGTTCCGCAGACAGCTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCCACCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTcgcccgc                                                                                                    >  2:2180363/1‑150 (MQ=255)
                                                   tGGAGATATTGCAGGATGGATTTGGTTTCCTCCGTTCCGCAGACAGCTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCCACCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGa                                                                                                 <  2:850999/150‑1 (MQ=255)
                                                   tGGAGATATTGCAGGATGGATTTGGTTTCCTCCGTTCCGCAGACAGCTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCCACCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGa                                                                                                 >  2:4441742/1‑150 (MQ=255)
                                                     gagaTATTGCAGGATGGATTTGGTTTCCTCCGTTCCGCAGACAGCTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCCACCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGaaa                                                                                               >  1:2209964/1‑150 (MQ=255)
                                                      agaTATTGCAGGATGGATTTGGTTTCCTCCGTTCCGCAGACAGCTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCCACCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAaag                                                                                              <  1:987971/150‑1 (MQ=255)
                                                       gaTATTGCAGGATGGATTTGGTTTCCTCCGTTCCGCAGACAGCTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCCACCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAaaga                                                                                             >  1:3441611/1‑150 (MQ=255)
                                                           ttGCAGGATGGATTTGGTTTCCTCCGTTCCGCAGACAGCTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCCACCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGt                                                                                         <  1:814076/150‑1 (MQ=255)
                                                               aGGATGGATTTGGTTTCCTCCGTTCCGCAGACAGCTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCCACCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGACGGTGAAc                                                                                     >  2:525398/1‑150 (MQ=255)
                                                               aGGATGGATTTGGTTTCCTCCGTTCCGCAGACAGCTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCCACCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGAAc                                                                                     >  2:2789786/1‑150 (MQ=255)
                                                               aGGATGGATTTGGTTTCCTCCGTTCCGCAGACAGCTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCCACCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGAAc                                                                                     <  2:3766495/150‑1 (MQ=255)
                                                               aGGATGGATTTGGTTTCCTCCGTTCCGCAGACAGCTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCCACCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGAAc                                                                                     <  2:4043962/150‑1 (MQ=255)
                                                                ggatggatTTGGTTTCCTCCGTTCCGCAGACAGCTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCCACCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGAACg                                                                                    >  1:4223734/1‑150 (MQ=255)
                                                                      atTTGGTTTCCTCCGTTCCGCAGACAGCTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCCGCCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGAACGCTAttt                                                                              >  1:2274693/1‑150 (MQ=255)
                                                                      atTTGGTTTCCTCCGTTCCGCAGACAGCTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCCGCCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGAACGCTAttt                                                                              >  1:2274938/1‑150 (MQ=255)
                                                                      atTTGGTTTCCTCCGTTCCGCAGACAGCTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCCACCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGAACGCTAttt                                                                              >  1:3290430/1‑150 (MQ=255)
                                                                      atTTGGTTTCCTCCGTTCCGCAGACAGCTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCCACCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGAACGCTAttt                                                                              >  1:3462737/1‑150 (MQ=255)
                                                                        ttGGTTTCCTCCGTTCCGCAGACAGCTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCCACCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGAACGCTATTTTg                                                                            >  2:2525791/1‑150 (MQ=255)
                                                                         tGGTTTCCTCCGTTCCGCAGACAGCTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCCACCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGAACGCTATTTTgc                                                                           <  2:3721630/150‑1 (MQ=255)
                                                                           gTTTCCTCCGTTCCGCAGACAGCTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCCACCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGAACGCTATTTTgcgc                                                                         >  1:3180602/1‑150 (MQ=255)
                                                                            tttCCTCCGTTCCGCAGACAGCTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCCACCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGAACGCTATTTTGCgct                                                                        <  2:1754415/150‑1 (MQ=255)
                                                                              tcctccGTTCCGCAGACAGCTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCCACCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGAACGCTATTTTGCgctgc                                                                      >  1:689328/1‑150 (MQ=255)
                                                                               cctccGTTCCGCAGACAGCTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCCACCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGAACGCTATTTTGCgctgct                                                                     <  1:233065/150‑1 (MQ=255)
                                                                               cctccGTTCCGCAGACAGCTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCCACCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGAACGCTATTTTGCgctgct                                                                     <  2:4532262/150‑1 (MQ=255)
                                                                               cctccGTTCCGCAGACAGCTCCGACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCCACCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGAACGCTATTTTGCgctgct                                                                     <  2:1646868/150‑1 (MQ=255)
                                                                                ctccGTTCCGCAGACAGCTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCCACCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGAACGCTATTTTGCGctgctg                                                                    <  1:2945630/150‑1 (MQ=255)
                                                                                      tCCGCAGACAGCTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCCACCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGAACGCTATTTTGCGCTGCTGAAAGtt                                                              <  1:2763535/150‑1 (MQ=255)
                                                                                      tCCGCAGACAGCTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCCACCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGAACGCTATTTTGCGCTGCTGAAAGtt                                                              <  1:3589349/150‑1 (MQ=255)
                                                                                       ccGCAGACAGCTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCCACCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGAACGCTATTTTGCGCTGCTGAAAGTTa                                                             <  2:2047875/150‑1 (MQ=255)
                                                                                        cGCAGACAGCTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCCACCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGAACGCTATTTTGCGCTGCTGAAAGTTaa                                                            >  2:4332630/1‑150 (MQ=255)
                                                                                        cGCAGACAGCTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCCACCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGAACGCTATTTTGCGCTGCTGAAAGTTaa                                                            <  1:2543254/150‑1 (MQ=255)
                                                                                         gCAGACAGCTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCCGCCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGAACGCTATTTTGCGCTGCTGAAAGTTAAc                                                           <  2:3243685/150‑1 (MQ=255)
                                                                                             aCAGCTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCCACCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGAACGCTATTTTGCGCTGCTGAAAGTTAACGAAg                                                       >  2:3651629/1‑150 (MQ=255)
                                                                                               aGCTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCCACCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGAACGCTATTTTGCGCTGCTGAAAGTTAACGAAGtt                                                     <  1:955285/150‑1 (MQ=255)
                                                                                                gCTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCCACCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGAACGCTATTTTGCTCTGCTGAAAGTTAACGAAGTTa                                                    <  2:3150112/150‑1 (MQ=255)
                                                                                                gCTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCCACCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGAACGCTATTTTGCGCTGCTGAAAGTTAACGAAGTTa                                                    >  2:4208691/1‑150 (MQ=255)
                                                                                                  tCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCCACCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGAACGCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAAc                                                  >  1:4151768/1‑150 (MQ=255)
                                                                                                     tACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCCACCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGAACGCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTc                                               >  1:1002827/1‑150 (MQ=255)
                                                                                                      aCCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCCACCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGAACGCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCg                                              <  1:394371/150‑1 (MQ=255)
                                                                                                         tCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCCACCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGAACGCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACa                                           >  1:44451/1‑150 (MQ=255)
                                                                                                          cGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCCACCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGAACGCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACaa                                          <  1:967919/150‑1 (MQ=255)
                                                                                                           gCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCCACCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGAACGCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACaaa                                         >  1:319488/1‑150 (MQ=255)
                                                                                                             cGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCCACCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGAACGCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAAcc                                       <  1:3134179/150‑1 (MQ=255)
                                                                                                              ggTCCTGATGACATCTACGTTTCCCCTAGCCAAATCCACCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGATCGCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCt                                      >  2:3052187/1‑150 (MQ=255)
                                                                                                               gTCCTGATGACATCTACGTTTCCCCTAGCCAAATCCACCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGAACGCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTg                                     <  2:771657/150‑1 (MQ=255)
                                                                                                                     atgaCATCTACGTTTCCCCTAGCCAAATCCGCCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGAACGCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACg                               >  2:1195878/1‑150 (MQ=255)
                                                                                                                        aCATCTACGTTTCCCCTAGCCAAATCCACCGTTTCAACCTCCGCACTGGTTATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGAACGATATTTTGCGCTGCTGAATGTTAACGAAGTTAACTTCGACAAACCTGAAAACGccc                            <  1:1116248/150‑1 (MQ=255)
                                                                                                                             tACGTTTCCCCTAGCCAAATCCACCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGAACGCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGcaac                       >  2:1839297/1‑150 (MQ=255)
                                                                                                                             tACGTTTCCCCTAGCCAAATCCACCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGAACGCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGcaac                       >  2:4134422/1‑150 (MQ=255)
                                                                                                                                  ttCCCCTCGCCAAATCCACCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGAACGATATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGCAACAAAAt                  <  2:3381410/150‑1 (MQ=255)
                                                                                                                                    ccccTAGCCAAATCCACCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGAACGCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGCAACAAAATcc                >  1:2239241/1‑150 (MQ=255)
                                                                                                                                        tAGCCAAATCCACCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGAACGCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGCAACAAAATCCTCtt            <  2:4159742/150‑1 (MQ=255)
                                                                                                                                        tAGCCAAATCCACCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGAACGCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGCAACAAAATCCTCtt            <  2:2041032/150‑1 (MQ=255)
                                                                                                                                        tAGCAAAATCCACCGTTTCAACCTCCGCACTGGTGATACCTTCTCTGTTAAGTTTCGCCCGCCGAAAGATGGTTAACGCTATTTTGCGCTGCTGAAAGTTAACGAATTTAACTTCGACAAACCTGAAAACGCCCGCAACAAAATCCTCtt            <  1:3009273/150‑1 (MQ=255)
                                                                                                                                          gCCAAATCCACCGTTTCAACCTCCGCACTGTTGATACCATCTATGGTAAGATTCGCCCGCCTAAATAAGGTTAACGCTATTTTGCGCTGCTTAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGCAACAAAATCCTCTTTg          <  2:3983980/150‑1 (MQ=255)
                                                                                                                                             aaaTCCACCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGAACGCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGCAACAAAATCCTCTTTgaga       >  1:3345126/1‑150 (MQ=255)
                                                                                                                                             aaaTCCACCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGAACGCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGCAACAAAATCCTCTTTgaga       >  1:4278252/1‑150 (MQ=255)
                                                                                                                                             aaaTCCACCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGAACGCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGCAACAAAATCCTCTTTgaga       >  1:1995402/1‑150 (MQ=255)
                                                                                                                                                tCCGCCTTTTAAACCTCCGAATTGGTTATAACATCTCTTGTAATTTTCGCCCGCCGAAAGATGTTTAACGATATTTTGCGCTGCTTAAATTTAACTAATTTAACTTCGAAAAACCTGAACACGCCCGCAACAAAATCCTCTTTGAGAACt    <  1:3291762/150‑1 (MQ=255)
                                                                                                                                                  cgccgTTTCAACCTCCGCACTGGTTATACAATCTCTGGTAAGATTCGCCCGCCGAAAGATGGTGAACGCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGCAACAAAATCCTCTTTGAGAACTTa  <  2:949989/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                    
TCCTGAAGCAGCACGCAAAGAGTGGCGAAGATATCTTTGGTGATGGCGTACTGGAGATATTGCAGGATGGATTTGGTTTCCTCCGTTCCGCAGACAGCTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCCGCCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGAACGCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGCAACAAAATCCTCTTTGAGAACTTA  >  CP009273/3959889‑3960184

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑