Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 4,068,208 C→A 88.9% G60G (GGC→GGA)  yihW → DeoR family putative transcriptional regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092734,068,2080CA88.9% 642.8 / 71.1 246G60G (GGC→GGA) yihWDeoR family putative transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base C (8/20);  new base A (114/102);  total (122/124)
Fisher's exact test for biased strand distribution p-value = 2.57e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 248 total aligned reads displayed.

CACGACCAACTCCTCATGCTGATCGCCGAGCGTGGGTATATGAATATTGATGAGCTGGCAAATCTGCTGGATGTCTCCACGCAGACGGTCCGCCGGGATATTCGTAAATTAAGCGAGCAAGGCCTGATTACGCGCCATCACGGTGGCGCGGGTCGGGCTTCCAGCGTCGTTAATACGGCGTTCGAGCAGCGTGAGGTTTCGCAAACCGAGGAAAAAAAAGCGATTGCCGAAGCGGTGGCAGACTATATTCCTGATGGTTCAACAATATTTATCACCATTGGTACGACTGTTGAG  >  CP009273/4068062‑4068355
                                                                                                                                                  |                                                                                                                                                   
cACGACCAACTCCTCATGCTGCTCGCCGAGCGTGGGTATATGAATATTGATGAGCTGGCAAATCTGCTGGATGTCTCCACGCAGACGGTCCGCCGGGATATTCGTAAATTAAGCGAGCAAGGCCTGATTACGCGCCATCACGGTGGcgcg                                                                                                                                                  <  1:1264349/150‑1 (MQ=255)
  cGACCAACTCCTCATGCTGATCGCCGAGCGTGGGTATATGAATATTGATGAGCTGGCAAATCTGCTGGATGTCTCCACGCAGACGGTCCGCCGGGATATTCGTAAATTAAGCGAGCAAGGCCTGATTACGCGCCATCACGGTGGAGCggg                                                                                                                                                <  1:4160344/150‑1 (MQ=255)
     ccAACTCCTCATGCTGATCGCCGAGCGTGGGTATATGAATATTGATGAGCTGGCAAATCTGCTGGATGTCTCCACGCAGACGGTCCGCCGGGATATTCGTAAATTAAGCGAGCAAGGCCTGATTACGCGCCATCACGGTGGAGCGGGTca                                                                                                                                             <  2:214324/150‑2 (MQ=255)
     ccAACTCCTCATGCTGATCGCCGAGCGTGGGTATATGAATATTGATGAGCTGGCAAATCTGCTGGATGTCTCCACGCAGACGGTCCGCCGGGATATTCGTAAATTAAGCGAGCAAGGCCTGATTACGCGCCATCACGGTGGAGCGGGTCg                                                                                                                                             >  1:4396061/1‑150 (MQ=255)
      cAACTCCTCATGCTGATCGCCGAGCGTGGGTATATGAATATTGATGAGCTGGCAAATCTGCTGGATGTCTCCACGCAGACGGTCCGCCGGGATATTCGTAAATTAAGCGAGCAAGGCCTGATTACGCGCCATCACGGTGGAGCGGGTCgg                                                                                                                                            >  2:4179302/1‑150 (MQ=255)
      cAACTCCTCATGCTGATCGCCGAGCGTGGGTATATGAATATTGATGAGCTGGCAAATCTGCTGGATGTCTCCACGCAGACGGTCCGCCGGGATATTCGTAAATTAAGCGAGCAAGGCCTGATTACGCGCCATCACGGTGGAGCGGGTCgg                                                                                                                                            >  1:3085439/1‑150 (MQ=255)
           cctcATGCTGATCGCCGAGCGTGGGTATATGAATATTGATGAGCTGGCAAATCTGCTGGATGTCTCCACGCAGACGGTCCGCCGGGATATTCGTAAATTAAGCGAGCAAGGCCTGATTACGCGCCATCACGGTGGAGCGGGTCGGGCTTc                                                                                                                                       <  2:1284410/150‑1 (MQ=255)
             tcATGCTGATCGCCGAGCGTGGGTATATGAATATTGATGAGCTGGCAAATCTGCTGGATGTCTCCACGCAGACGGTCCGCCGGGATATTCGTAAATTAAGCGAGCAAGGCCTGATTACGCGCCATCACGGTGGCGCGGGTCGGGCTTCCa                                                                                                                                     >  2:1958756/1‑150 (MQ=255)
             tcATGCTGATCGCCGAGCGTGGGTATATGAATATTGATGAGCTGGCAAATCTGCTGGATGTCTCCACGCAGACGGTCCGCCGGGATATTCGTAAATTAAGCGAGCAAGGCCTGATTACGCGCCATCACGGTGGCGCGGGTCGGGCTTCCa                                                                                                                                     >  2:781887/1‑150 (MQ=255)
             tcATGCTGATCGCCGAGCGTGGGTATATGAATATTGATGAGCTGGCAAATCTGCTGGATGTCTCCACGCAGACGGTCCGCCGGGATATTCGTAAATTAAGCGAGCAAGGCCTGATTACGCGCCATCACGGTGGAGCGGGTCGGGCTTCCa                                                                                                                                     <  1:429413/150‑1 (MQ=255)
                tGCTGATCGCCGAGCGTGGGTATATGAATATTGATGAGCTGGCAAATCTGCTGGATGTCTCCACGCAGACGGTCCGCCGGGATATTCGTAAATTAAGCGAGCAAGGCCTGATTACGCGCCATCACGGTGGAGCGGGTCGGGCTTCCAGcg                                                                                                                                  >  2:3820014/1‑150 (MQ=255)
                  cTGATCGCCGAGCGTGGGTATATGAATATTGATGAGCTGGCAAATCTGCTGGATGTCTCCACGCAGACGGTCCGCCGGGATATTCGTAAATTAAGCGAGCAAGGCCTGATTACGCGCCATCACGGTGGAGCGGGTCGGGCTTCCAGcgtc                                                                                                                                <  1:1692037/150‑1 (MQ=255)
                   tGATCGCCGAGCGTGGGTATATTAATATTGATGAGCTGGCAAATCTGCTGGATGTCTCCACGCAGACGGTCCGCCGGGATATTCGTAAATTAAGCGAGCAAGGCCTGATTACGCGCCATCACGGTGGAGCGGGTCGGGCTTCCAGcgtcg                                                                                                                               >  1:1843545/1‑150 (MQ=255)
                    gATCGCCGAGCGTGGGTATATGAATATTGATGAGCTGGCAAATCTGCTGGATGTCTCCACGCAGACGGTCCGCCGGGATATTCGTAAATTAAGCGAGCAAGGCCTGATTACGCGCCATCACGGTGGAGCGGGTCGGGCTTCCAGcgtcgt                                                                                                                              >  1:3902121/1‑150 (MQ=255)
                     aTCGCCGAGCGTGGGTATATGAATATTGATGAGCTGGCAAATCTGCTGGATGTCTCCACGCAGACGGTCCGCCGGGATATTCGTAAATTAAGCGAGCAAGGCCTGATTACGCGCCATCACGGTGGAGCGGGTCGGGCTTCCAGCGTCGtt                                                                                                                             >  2:2858209/1‑150 (MQ=255)
                      tCGCCGAGCGTGGGTATATGAATATTGATGAGCTGGCAAATCTGCTGGATGTCTCCACGCAGACGGTCCGCCGGGATATTCGTAAATTAAGCGAGCAAGGCCTGATTACGCGCCATCACGGTGGAGCGGGTCGGGCTTCCAGCGTCGTTa                                                                                                                            <  2:2540268/150‑1 (MQ=255)
                        gCCGAGCGTGGGTATATGAATATTGATGAGCTGGCAAATCTGCTGGATGTCTCCACGCAGACGGTCCGCCGGGATATTCGTAAATTAAGCGAGCAAGGCCTGATTACGCGCCATCACGGTGGCGCGGGTCGGGCTTCCAGCGTCGTTAAt                                                                                                                          <  2:4405156/150‑1 (MQ=255)
                         ccGAGCGTGGGTATATGAATATTGATGAGCTGGCAAATCTGCTGGATGTCTCCACGCAGACGGTCCGCCGGGATATTCGTAAATTAAGCGAGCAAGGCCTGATTACGCGCCATCACGGTGGAGCGGGTCGGGCTTCCAGCGTCGTTAATa                                                                                                                         <  1:3800916/150‑1 (MQ=255)
                           gAGCGTGGGTATATGAATATTGATGAGCTGGCAAATCTGCTGGATGTCTCCACGCAGACGGTCCGCCGGGATATTCGTAAATTAAGCGAGCAAGGCCTGATTACGCGCCATCACGGTGGAGCGGGTCGGGCTTCCAGCGTCGTTAATACg                                                                                                                       <  2:3983413/150‑1 (MQ=255)
                            aGCGTGGGTATATGAATATTGATGAGCTGGCAAATCTGCTGGATGTCTCCACGCAGACGGTCCGCCGGGATATTCGTAAATTAAGCGAGCAAGGCCTGATTACGCGCCATCACGGTGGAGCGGGTCGGGCTTCCAGCGTCGTTAATACgg                                                                                                                      >  2:1022103/1‑150 (MQ=255)
                             gCGTGGGTATATGAATATTGATGAGCTGGCAAATCTGCTGGATGTCTCCACGCAGACGGTCCGCCGGGATATTCGTAAATTAAGCGAGCAAGGCCTGATTACGCGCCATCACGGTGGAGCGGGTCGGGCTTCCAGCGTCGTTAATACGGc                                                                                                                     >  2:4364643/1‑150 (MQ=255)
                              cGTGGGTATATGAATATTGATGAGCTGGCAAATCTGCTGGATGTCTCCACGCAGACGGTCCGCCGGGATATTCGTAAATTAAGCGAGCAAGGCCTGATTACGCGCCATCACGGTGGAGCGGGTCGGGCTTCCAGCGTCGTTAATACGGCg                                                                                                                    >  1:1532605/1‑150 (MQ=255)
                              cGTGGGTATATGAATATTGATGAGCTGGCAAATCTGCTGGATGTCTCCACGCAGACGGTCCGCCGGGATATTCGTAAATTAAGCGAGCAAGGCCTGATTACGCGCCATCACGGTGGAGCGGGTCGGGCTTCCAGCGTCGTTAATACGGCg                                                                                                                    <  1:1673092/150‑1 (MQ=255)
                              cGTGGGTATATGAATATTGATGAGCTGGCAAATCTGCTGGATGTCTCCACGCAGACGGTCCGCCGGGATATTCGTAAATTAAGCGAGCAAGGCCTGATTACGCGCCATCACGGTGGAGCGGGTCGGGCTTCCAGCGTCGTTAATACGGCg                                                                                                                    >  1:3511558/1‑150 (MQ=255)
                               gTGGGTATATGAATATTGATGAGCTGGCAAATCTGCTGGATGTCTCCACGCAGACGGTCCGCCGGGATATTCGTAAATTAAGCGAGCAAGGCCTGATTACGCGCCATCACGGTGGAGCGGGTCGGGCTTCCAGCGTCGTTAATACGGCGt                                                                                                                   >  2:1689190/1‑150 (MQ=255)
                                tGGGTATATGAATATTGATGAGCTGGCAAATCTGCTGGATGTCTCCACGCAGACGGTCCGCCGGGATATTCGTAAATTAAGCGAGCAAGGCCTGATTACGCGCCATCACGGTGGAGCGGGTCGGGCTTCCAGCGTCGTTAATACGGCGtt                                                                                                                  >  2:1121206/1‑150 (MQ=255)
                                   gTATATGAATATTGATGAGCTGGCAAATCTGCTGGATGTCTCCACGCAGACGGTCCGCCGGGATATTCGTAAATTAAGCGAGCAAGGCCTGATTACGCGCCATCACGGTGGAGCGGGTCGGGCTTCCAGCGTCGTTAATACGGCGTTCGa                                                                                                               >  2:4529328/1‑150 (MQ=255)
                                   gTATATGAATATTGATGAGCTGGCAAATCTGCTGGATGTCTCCACGCAGACGGTCCGCCGGGATATTCGTAAATTAAGCGAGCAAGGCCTGATTACGCGCCATCACGGTGGAGCGGGTCGGGCTTCCAGCGTCGTTAATACGGCGTTCGa                                                                                                               >  2:4529319/1‑150 (MQ=255)
                                     atatGAATATTGATGAGCTGGCAAATCTGCTGGATGTCTCCACGCAGACGGTCCGCCGGGATATTCGTAAATTAAGCGAGCAAGGCCTGATTACGCGCCATCACGGTGGAGCGGGTCGGGCTTCCAGCGTCGTTAATACGGCGTTCGagc                                                                                                             <  1:657385/150‑1 (MQ=255)
                                         gAATATTGATGAGCTGGCAAATCTGCTGGATGTCTCCACGCAGACGGTCCGCCGGGATATTCGTAAATTAAGCGAGCAAGGCCTGATTACGCGCCATCACGGTGGAGCGGGTCGGGCTTCCAGCGTCGTTAATACGGCGTTCGAGCAGCg                                                                                                         >  2:2157759/1‑150 (MQ=255)
                                          aaTATTGATGAGCTGGCAAATCTGCTGGATGTCTCCACGCAGACGGTCCGCCGGGATATTCGTAAATTAAGCGAGCAAGGCCTGATTACGCGCCATCACGGTGGAGCGGGTCGGGCTTCCAGCGTCGTTAATACGGCGTTCGAGCAGCGt                                                                                                        >  1:864509/1‑150 (MQ=255)
                                           atatTGATGAGCTGGCAAATCTGCTGGATGTCTCCACGCAGACGGTCCGCCGGGATATTCGTAAATTAAGCGAGCAAGGCCTGATTACGCGCCATCACGGTGGAGCGGGTCGGGCTTCCAGCGTCGTTAATACGGCGTTCGAGCAGCGTg                                                                                                       <  2:4290337/150‑1 (MQ=255)
                                             atTGATGAGCTGGCAAATCTGCTGGATGTCTCCACGCAGACGGTCCGCCGGGATATTCGTAAATTAAGCGAGCAAGGCCTGATTACGCGCCATCACGGTGGAGCGGGTCGGGCTTCCAGCGTCGTTAATACGGCGTTCGAGCAGCGTGAg                                                                                                     >  2:534737/1‑150 (MQ=255)
                                                gatgaGCTGGCAAATCTGCTGGATGTCTCCACGCAGACGGTCCGCCGGGATATTCGTAAATTAAGCGAGCAAGGCCTGATTACGCGCCATCACGGTGGAGCGGGTCGGGCTTCCAGCGTCGTTAATACGGCGTTCGAGCAGCGTGAGGtt                                                                                                  >  1:2722075/1‑150 (MQ=255)
                                                gatgaGCTGGCAAATCTGCTGGATGTCTCCACGCAGACGGTCCGCCGGGATATTCGTAAATTAAGCGAGCAAGGCCTGATTACGCGCCATCACGGTGGAGCGGGTCGGGCTTCCAGCGTCGTTAATACGGCGTTCGAGCAGCGTGAGGtt                                                                                                  <  1:4179302/150‑1 (MQ=255)
                                                 atgaGCTGGCAAATCTGCTGGATGTCTCCACGCAGACGGTCCGCCGGGATATTCGTAAATTAAGCGAGCAAGGCCTGATTACGCGCCATCACGGTGGCGCGGGTCGGGCTTCCAGCGTCGTTAATACGGCGTTCGAGCAGCGTGAGGttt                                                                                                 <  1:2140738/150‑1 (MQ=255)
                                                 atgaGCTGGCAAATCTGCTGGATGTCTCCACGCAGACGGTCCGCCGGGATATTCGTAAATTAAGCGAGCAAGGCCTGATTACGCGCCATCACGGTGGAGCGGGTCGGGCTTCCAGCGTCGTTAATACGGCGTTCGAGCAGCGTGAGGttt                                                                                                 <  1:476977/150‑1 (MQ=255)
                                                  tgaGCTGGCAAATCTGCTGGATGTCTCCACGCAGACGGTCCGCCGGGATATTCGTAAATTAAGCGAGCAAGGCCTGATTACGCGCCATCACGGTGGAGCGGGTCGGGCTTCCAGCGTCGTTAATACGGCGTTCGAGCAGCGTGAGGTTTc                                                                                                <  2:596071/150‑1 (MQ=255)
                                                  tgaGCTGGCAAATCTGCTGGATGTCTCCACGCAGACGGTCCGCCGGGATATTCGTAAATTAAGCGAGCAAGGCCTGATTACGCGCCATCACGGTGGAGCGGGTCGGGCTTCCAGCGTCGTTAATACGGCGTTCGAGCAGCGTGAGGTTTc                                                                                                <  2:3104021/150‑1 (MQ=255)
                                                            aaTCTGCTGGATGTCTCCACGCAGACGGTCCGCCGGGATATTCGTAAATTAAGCGAGCAAGGCCTGATTACGCGCCATCACGGTGGAGCGGGTCGGGCTTCCAGCGTCGTTAATACGGCGTTCGAGCAGCGTGAGGTTTCGCAAACCGAg                                                                                      >  2:2245890/1‑150 (MQ=255)
                                                            aaTCTGCTGGATGTCTCCACGCAGACGGTCCGCCGGGATATTCGTAAATTAAGCGAGCAAGGCCTGATTACGCGCCATCACGGTGGAGCGGGTCGGGCTTCCAGCGTCGTTAATACGGCGTTCGAGCAGCGTGAGGTTTCGCAAACCGAg                                                                                      >  2:2898704/1‑150 (MQ=255)
                                                               ctgctgGATGTCTCCACGCAGACGGTCCGCCGGGATATTCGTAAATTAAGCGAGCAAGGCCTGATTACGCGCCATCACGGTGGAGCGGGTCGGGCTTCCAGCGTCGTTAATACGGCGTTCGAGCAGCGTGAGGTTTCGCAAACCGAGGaa                                                                                   <  2:4038667/150‑1 (MQ=255)
                                                                 gctgGATGTCTCCACGCAGACGGTCCGCCGGGATATTCGTAAATTAAGCGAGCAAGGCCTGATTACGCGCCATCACGGTGGCGCGGGTCGGGCTTCCAGCGTCGTTAATACGGCGTTCGCGCCGCGTGAGGTTTCGCACACCGAGGaaaa                                                                                 >  1:2648421/1‑150 (MQ=255)
                                                                    ggATGTCTCCACGCAGACGGTCCGCCGGGATATTCGTAAATTAAGCGAGCAAGGCCTGATTACGCGCCATCACGGTGGAGCGGGTCGGGCTTCCAGCGTCGTTAATACGGCGTTCGAGCAGCGTGAGGTTTCGCAAACCGAGGaaaaaaa                                                                              >  2:3287394/1‑150 (MQ=255)
                                                                      aTGTCTCCACGCAGACGGTCCGCCGGGATATTCGTAAATTAAGCGAGCAAGGCCTGATTACGCGCCATCACGGTGGAGCGGGTCGGGCTTCCAGCGTCGTTAATACGGCGTTCGAGCAGCGTGAGGTTTCGCAAACCGAGGAAAAAAAAg                                                                            <  2:3438719/150‑1 (MQ=255)
                                                                           tcCACGCAGACGGTCCGCCGGGATATTCGTAAATTAAGCGAGCAAGGCCTGATTACGCGCCATCACGGTGGAGCGGGTCGGGCTTCCAGCGTCGTTAATACGGCGTTCGAGCAGCGTGAGGTTTCGCAAACCGAGGAAAAAAAAGCGAtt                                                                       <  2:1067274/150‑1 (MQ=255)
                                                                            ccACGCAGACGGTCCGCCGGGATATTCGTAAATTAAGCGAGCAAGGCCTGATTACGCGCCATCACGGTGGAGCGGGTCGGGCTTCCAGCGTCGTTAATACGGCGTTCGAGCAGCGTGAGGTTTCGCAAACCGAGGAAAAAAAAGCGATTg                                                                      >  1:3916460/1‑150 (MQ=255)
                                                                                 cAGACGGTCCGCCGGGATATTCGTAAATTAAGCGAGCAAGGCCTGATTACGCGCCATCACGGTGGAGCGGGTCGGGCTTCCAGCGTCGTTAATACGGCGTTCGAGCAGCGTGAGGTTTCGCAAACCGAGGAAAAAAAAGCGATTGCCGaa                                                                 >  2:1863074/1‑150 (MQ=255)
                                                                                  aGACGGTCCGCCGGGATATTCGTAAATTAAGCGAGCAAGGCCTGATTACGCGCCATCACGGTGGAGCGGGTCGGGCTTCCAGCGTCGTTAATACGGCGTTCGAGCAGCGTGTGGTTTCGCAAACCGAGGAAAAAAAAGCGATTGCCGAAg                                                                <  1:331504/150‑1 (MQ=255)
                                                                                   gatggTCCGCCGGGATATTCGTAAATTAAGCGAGCAAGGCCTGATTACGCGCCATCACGGTGGAGCGGGTCGGGCTTCCAGCGTCGTTAATACGGCGTTCGAGCAGCGTGAGGTTTCGCAAACCGAGGAAAAAAAAGCGATTGCCGAAGc                                                               >  1:1559278/4‑150 (MQ=255)
                                                                                   gACGGTCCGCCGGGATATTCGTAAATTAAGCGAGCAAGGCCTGATTACGCGCCATCACGGTGGAGCGGGTCGGGCTTCCAGCGTCGTTAATACGGCGTTCGAGCAGCGTGAGGTTTCGCAAACCGAGGAAAAAAAAGCGATTGCCGAAGc                                                               >  1:1558418/1‑150 (MQ=255)
                                                                                    aCGGTCCGCCGGGATATTCGTAAATTAAGCGAGCCCGGCCTGATTACGCGCCATCCCGGTGGAGCGGGTCGGGCTGCCAGCGTCGTTAATACGGCGTTCGAGCAGCGTGAGGTTTCGCAAACCGAGGAAAAAAAAGCGCTTGCCGAAGCg                                                              <  2:2039675/150‑1 (MQ=255)
                                                                                     cGGTCCGCCGGGATATTCGTAAATTAAGCGAGCAAGGCCTGATTACGCGCCATCACGGTGGAGCGGGTCGGGCTTCCAGCGTCGTTAATACGGCGTTCGAGCAGCGTGAGGTTTCGCAAACCGAGGAAAAAAAAGCGATTGCCGAAGCgg                                                             >  1:3944089/1‑150 (MQ=255)
                                                                                      ggTCCGCCGGGATATTCGTAAATTAAGCGAGCAAGGCCTGATTACGCGCCATCACGGTGGAGCGGGTCGGGCTTCCAGCGTCGTTAATACGGCGTTCGAGCAGCGTGAGGTTTCGCAAACCGAGGAAAAAAAAGCGATTGCCGAAGCGGt                                                            >  2:1222504/1‑150 (MQ=255)
                                                                                          cgccgGGATATTCGTAAATTAAGCGAGCAAGGCCTGATTACGCGCCATCACGGTGGAGCGGGTCGGGCTTCCAGCGTCGTTAATACGGCGTTCGAGCTGCGTGAGGTTTCGCAAACCGAGTAAAAAAAAGCGATTGCCGAAGCGGTGGCa                                                        <  1:3624360/150‑1 (MQ=255)
                                                                                          cgccgGGATATTCGTAAATTAAGCGAGCAAGGCCTGATTACGCGCCATCACGGTGGAGCGGGTCGGGCTTCCAGCGTCGTTAATACGGCGTTCGAGCAGCGTGAGGTTTCGCAAACCGAGGAAAAAAAAGCGATTGCCGAAGCGGTGGCa                                                        <  2:3318876/150‑1 (MQ=255)
                                                                                           gccgGGATATTCGTAAATTAAGCGAGCAAGGCCTGATTACGCGCCATCACGGTGGAGCGGGTCGGGCTTCCAGCGTCGTTAATACGGCGTTCGAGCAGCGTGAGGTTTCGCAAACCGAGGAAAAAAAAGCGATTGCCGAAGCGGTGGCAg                                                       >  1:1195129/1‑150 (MQ=255)
                                                                                            ccgGGATATTCGTAAATTAAGCGAGCAAGGCCTGATTACGCGCCATCACGGTGGAGCGGGTCGGGCTTCCAGCGTCGTTAATACGGCGTTCGAGCAGCGTGAGGTTTCGCAAACCGAGGAAAAAAAAGAGATTGCCGAAGCGGTGGCAGa                                                      >  2:125090/1‑150 (MQ=255)
                                                                                              gggATATTCGTAAATTAAGCGAGCAAGGCCTGATTACGCGCCATCACGGTGGCGCGGGTCGGGCTTCCAGCGTCGTTAATACGGCGTTCGAGCAGCGTGAGGTTTCGCAAACCGAGGAAAAAAAAGCGATTGCCGAAGCGGTGGCAGACt                                                    <  1:3995441/150‑1 (MQ=255)
                                                                                               ggATATTCGTAAATTAAGCGAGCAAGGCCTGATTACGCGCCATCACGGTGGAGCGGGTCGGGCTTCCAGCGTCGTTAATACGGCGTTCGAGCAGCGTGAGGTTTCGCAAACCGAGGAAAAAAAAGCGATTGCCGAAGCGGTGGCAGACta                                                   <  2:3553550/150‑1 (MQ=255)
                                                                                                gATATTCGTAAATTAAGCGAGCAAGGCCTGATTACGCGCCATCACGGTGGAGCGGGTCGGGCTTCCAGCGTCGTTAATACGGCGTTCGAGCAGCGTGAGGTTTCGCAAACCGAGGAAAAAAAAGCGATTGCCGAAGCGGTGGCAGACtat                                                  >  2:4278166/1‑150 (MQ=255)
                                                                                                  tatTCGTAAATTAAGCGAGCAAGGCCTGATTACGCGCCATCACGGTGGAGCGGGTCGGGCTTCCAGCGTCGTTAATACGGCGTTCGAGCAGCGTGAGGTTTCGCAAACCGAGGAAAAAAAAGAGATTGCCGAAGCGGTGGCAGACtaaaa                                                >  1:2099532/1‑147 (MQ=255)
                                                                                                   atTCGTAAATTAAGCGAGCAAGGCCTGATTACGCGCCATCACGGTGGAGCGGGTCGGGCTTCCAGCGTCGTTAATACGGCGTTCGAGCAGCGTGAGGTTTCGCAAACCGAGGAAAAAAAAGCGATTGCCGAAGCGGTGGCAGACTATAtt                                               <  2:3397954/150‑1 (MQ=255)
                                                                                                      cGTAAATTAAGCGAGCAAGGCCTGATTACGCGCCATCACGGTGGAGCGGGTCGGGCTTCCAGCGTCGTTAATACGGCGTTCGAGCAGCGTGAGGTTTCGCAAACCGAGGAAAAAAAAGCGATTGCCGAAGCGGTGGCAGACTATATTCCt                                            <  1:922817/150‑1 (MQ=255)
                                                                                                      cGTAAATTAAGCGAGCAAGGCCTGATTACGCGCCATCACGGTGGAGCGGGTCGGGCTTCCAGCGTCGTTAATACGGCGTTCGAGCAGCGTGAGGTTTCGCAAACCGAGGAAAAAAAAGCGATTGCCGAAGCGGTGGCAGACTATATTCCt                                            >  2:63199/1‑150 (MQ=255)
                                                                                                      cGTAAATTAAGCGAGCAAGGCCTGATTACGCGCCATCACGGTGGAGCGGGTCGGGCTTCCAGCGTCGTTAATACGGCGTTCGAGCAGCGTGAGGTTTCGCAAACCGAGGAAAAAAAAGCGATTGCCGAAGCGGTGGCAGACTATATTCCt                                            <  2:268643/150‑1 (MQ=255)
                                                                                                      cGTAAATTAAGCGAGCAAGGCCTGATTACGCGCCATCACGGTGGAGCGGGTCGGGCTTCCAGCGTCGTTAATACGGCGTTCGAGCAGCGTGAGGTTTCGCAAACCGAGGAAAAAAAAGCGATTGCCGAAGCGGTGGCAGACTATATTCCt                                            <  1:260036/150‑1 (MQ=255)
                                                                                                        tAAATTAAGCGAGCAAGGCCTGATTACGCGCCATCACGGTGGAGCGGGTCGGGCTTCCAGCGTCGTTAATACGGCGTTCGAGCAGCGTGAGGTTTCGCAAACCGAGGAAAAAAAAGCGATTGCCGAAGCGGTGGCAGACTATATTCCTGa                                          >  2:3220955/1‑150 (MQ=255)
                                                                                                          aaTTAAGCGAGCAAGGCCTGATTACGCGCCATCACGGTGGAGCGGGTCGGGCTTCCAGCGTCGTTAATACGGCGTTCGAGCAGCGTGAGGTTTCGCAAACCGAGGAAAAAAAAGCGATTGCCGAAGCGGTGGCAGACTATATTCCTGATg                                        <  1:3626308/150‑1 (MQ=255)
                                                                                                          aaTTAAGCGAGCAAGGCCTGATTACGCGCCATCACGGTGGAGCGGGTCGGGCTTCCAGCGTCGTTAATACGGCGTTCGAGCAGCGTGAGGTTTCGCAAACCGAGGAAAAAAAAGCGATTGCCGAAGCGGTGGCAGACTATATTCCTGATg                                        >  1:358150/1‑150 (MQ=255)
                                                                                                           aTTAAGCGAGCAAGGCCTGATTACGCGCCATCACGGTGGAGCGGGTCGGGCTTCCAGCGTCGTTAATACGGCGTTCGAGCAGCGTGAGGTTTCGCAAACCGAGGAAAAAAAAGCGATTGCCGAAGCGGTGGCAGACTATATTCCTGATgg                                       >  1:4058578/1‑150 (MQ=255)
                                                                                                            ttAAGCGAGCAAGGCCTGATTACGCGCCATCACGGTGGAGGGGGTCGGGCTTCCAGCGTCGTTAATACGGCGTTCGAGCAGCGTGAGGTTTCGCAAACCGAGGAAAAAAAAGCGATTGCCGAAGCGGTGGCAGACTATATTCCTGATGGt                                      <  1:1289449/150‑1 (MQ=255)
                                                                                                            ttAAGCGAGAAAGGCCTGATTACGCGCCATCACGGTGGAGCGGGTCGGGCTTCCAGCGTCGTTAATACGGCGTTCGAGCAGCGTGAGGTTTCGCAAACCGAGGAAAAAAAAGCGATTGCCGAAGCGGTGGCAGACTATATTCCTGATGGt                                      <  1:1010365/150‑1 (MQ=255)
                                                                                                             tacgCGAGCACGGCCTGATTACGCGCCATCACGGTGGTGGGGGTCGGGCTTCCCGCGTCGTTAATACGGCGTGCGAGCAGCGTGAGGTTTCGCACACCGAGGAAAAAAAAGCGATTGCCGAACCGGTGGCAGACTATATTCCTGATGGtt                                     <  1:3067946/147‑1 (MQ=255)
                                                                                                              aaGCGAGCAAGGCCTGATTACGCGCCAACCCGGTGGAGCGGGTCGGGCTTCCAGCGTCGTTACTACGGCGTTCGAGCAGCGTGAGGTTTCGCAAACCGAGGATAAAAAAGCGATTGCCGAAGCGGTGGCAGACTATATTCCTGATGGTTc                                    <  2:1446649/150‑1 (MQ=255)
                                                                                                                gCGAGCAAGGCCTGATTACGCGCCATCACGGTGGAGCGGGTCGGGCTTCCAGCGTCGTTAATACGGCGTTCGAGCAGCGTGAGGTTTCGCAAACCGAGGAAAAAAAAGCGATTGCCGAAGCGGTGGCAGACTATATTCCTGATGGTTcaa                                  >  2:2717054/1‑150 (MQ=255)
                                                                                                                 cGAGCAAGGCCTGATTACGCGCCATCACGGTGGAGCGGGTCGGGCTTCCAGCGTCGTTAATACGGCGTTCGAGCAGCGTGAGGTTTCGCAAACCGAGGAAAAAAAAGCGATTGCCGAAGCGGTGGCAGACTATATTCCTGATGGTTcaac                                 >  1:4521847/1‑150 (MQ=255)
                                                                                                                 cGAGCAAGGCCTGATTACGCGCCATCACGGTGGAGCGGGTCGGGCTTCCAGCGTCGTTAATACGGCGTTCGAGCAGCGTGAGGTTTCGCAAACCGAGGAAAAAAAAGCGATTGCCGAAGCGGTGGCAGACTATATTCCTGATGGTTcaac                                 >  1:2248966/1‑150 (MQ=255)
                                                                                                                  gAGCAAGGCCTGATTACGCGCCATCACGGTGGAGCGGGTCGGGCTTCCAGCGTCGTTAATACGGCGTTCGAGCAGCGTGAGGTTTCGCAAACCGAGGAAAAAAAAGCGATTGCCGAAGCGGTGGCAGACTATATTCCTGATGGTTcaaca                                >  1:1016287/1‑150 (MQ=255)
                                                                                                                  gAGCAAGGCCTGATTACGCGCCATCACGGTGGAGCGGGTCGGGCTTCCAGCGTCGTTAATACGGCGTTCGAGCAGCGTGAGGTTTCGCAAACCGAGGAAAAAAAAGCGATTGCCGAAGCGGTGGCAGACTATATTCCTGATGGTTcaaca                                >  1:1187346/1‑150 (MQ=255)
                                                                                                                  gAGCAAGGCCTGATTACGCGCCATCACGGTGGAGCGGGTCGGGCTTCCAGCGTCGTTAATACGGCGTTCGAGCAGCGTGAGGTTTCGCAAACCGAGGAAAAAAAAGCGATTGCCGAAGCGGTGGCAGACTATATTCCTGATGGTTcaaca                                >  1:282082/1‑150 (MQ=255)
                                                                                                                  gAGCAAGGCCTGATTACGCGCCATCACGGTGGAGCGGGTCGGGCTTCCAGCGTCGTTAATACGGCGTTCGAGCAGCGTGAGGTTTCGCAAACCGAGGAAAAAAAAGCGATTGCCGAAGCGGTGGCAGACTATATTCCTGATGGTTcaaca                                >  1:2909476/1‑150 (MQ=255)
                                                                                                                   aGCAAGGCCTGATTACGCGCCATCACGGTGGAGCGGGTCGGGCTTCCAGCGTCGTTAATACGGCGTTCGAGCAGCGTGAGGTTTCGCAAACCGAGGAAAAAAAAGCGATTGCCGAAGCGGTGGCAGACTATATTCCTGATGGTTcaacaa                               >  2:1611262/1‑150 (MQ=255)
                                                                                                                   aGCAAGGCCTGATTACGCGCCATCACGGTGGAGCGGGTCGGGCTTCCAGCGTCGTTAATACGGCGTTCGAGCAGCGTGAGGTTTCGCAAACCGAGGAAAAAAAAGCGATTGCCGAAGCGGTGGCAGACTATATTCCTGATGGTTcaacaa                               >  2:3889181/1‑150 (MQ=255)
                                                                                                                       aGGCCTGATTACGCGCCATCACGGTGGAGCGGGTCGGGCTTCCAGCGTCGTTAATACGGCGTTCGAGCAGCGTGAGGTTTCGCAAACCGAGGAAAAAAAAGCGATTGCCGAAGCGGTGGCAGACTATATTCCTGATGGTTCAACAATAtt                           <  1:1022103/150‑1 (MQ=255)
                                                                                                                       aGGCCTGATTACGCGCCATCACGGTGGAGCGGGTCGGGCTTCCAGCGTCGTTAATACGGCGTTCGAGCAGCGTGAGGGTTCGCAAACCGCGGAAAAAAAAGCGATTGCCGAAGCGGTGGCCGCCTATATTCCTGATGGTTCAACAATAtt                           >  1:3437766/1‑150 (MQ=255)
                                                                                                                         gCCTGATTACGCGCCATCACGGTGGCGCGGGTCGGGCTTCCAGCGTCGTTAATACGGCGTTCGAGCAGCGTGAGGTTTCGCAAACCGAGGAAAAAAAAGCGATTGCCGAAGCGGTGGCAGAGAATATTCATGATGGTTAAACAAAATTTa                         >  1:1465962/1‑150 (MQ=255)
                                                                                                                            tGATTACGCGCCATCACGGTGGAGCGGGTCGGGCTTCCAGCGTCGTTAATACGGCGTTCGAGCAGCGTGAGGTTTCGCAAACCGAGGAAAAAAAAGCGATTGCCGAAGCGGTGGCAGACTATATTCCTGATGGTTCAACAATATTTATCa                      <  1:637464/150‑1 (MQ=255)
                                                                                                                             gATTACGCGCCATCCCGGTGGCGCGGGTCGGGCTTCCAGCGTCGTTAATACGGCGTTCGAGCAGCGTGAGGTTTCGCAACCCGAGGAAAAAAAAGCGATTGCCGAAGCGGTGGCAGACTATATTCCTGATGGTTCAACAATATTTATCAc                     <  1:2765769/150‑1 (MQ=255)
                                                                                                                             gATTACGCGCCATCCCGGTGGCGCGGGTCGGGCTTCCAGCGTCGTTAATACGGCGTTCGAGCAGCGTGAGGTTTCGCAAACCGAGGAAAAAAAAGCGATTGCCGAAGCGGTGGCAGACTATATTCCTGATGGTTCAACAATATTTATCAc                     <  1:2765725/150‑1 (MQ=255)
                                                                                                                               ttACGCGCCATCACGGTGGAGCGGGTCGGGCTTCCAGCGTCGTTAATACGGCGTTCGAGCAGCGTGAGGTTTCGCAAACCGAGGAAAAAAAAGCGATTGCCGAAGCGGTGGCAGACTATATTCCTGATGGTTCAACAATATTTATCACCa                   <  2:1532605/150‑1 (MQ=255)
                                                                                                                                 aCGCGCCATCACGGTGGAGCGGGTCGGGCTTCCAGCGTCGTTAATACGGCGTTCGAGCAGCGTGAGGTTTCGCAAACCGAGGAAAAAAAAGCGATTGCCGAAGCGGTGGCAGACTATATTCCTGATGGTTCAACAATATTTATCACCAtt                 <  1:679553/150‑1 (MQ=255)
                                                                                                                                  cgcgCCATCACGGTGGAGCGGGTCGGGCTTCCAGCGTCGTTAATACGGCGTTCGAGCAGCGTGAGGTTTCGCAAACCGAGGAAAAAAAAGCGATTGCCGAAGCGGTGGCAGACTATATTCCTGATGGTTCAACAATATTTATCACCATTg                <  1:534737/150‑1 (MQ=255)
                                                                                                                                    cgcCATCACGGTGGCGCGGGTCGGGCTTCCAGCGTCGTTAATACGGCGTTCGAGCAGCGTGAGGTTTCGCAAACCGAGGAAAAAAAAGCGATTGCCGAAGCGGTGGCAGACTATATTCCTGATGGTTCAACAATATTTATCACCATTGGt              <  2:3228699/150‑1 (MQ=255)
                                                                                                                                    cgcCATCACGGTGGAGCGGGTCGGGCTTCCAGCGTCGTTAATACGGCGTTCGAGCAGCGTGAGGTTTCGCAAACCGAGGAAAAAAAAGCGATTGCCGAAGCGGTGGCAGACTATATTCCTGATGGTTCAACAATATTTATCACCATTGGt              <  1:809361/150‑1 (MQ=255)
                                                                                                                                     gcCATCACGGTGGAGCGGGTCGGGCTTCCAGCGTCGTTAATACGGCGTTCGAGCAGCGTGAGGTTTCGCAAACCGAGGAAAAAAAAGCGATTGCCGAAGCGGTGGCAGACTATATTCCTGATGGTTCAACAATATTTATCACCATTGGTa             >  1:4109896/1‑150 (MQ=255)
                                                                                                                                     gcCATCACGGTGGAGCGGGTCGGGCTTCCAGCGTCGTTAATACGGCGTTCGAGCAGCGTGAGGTTTCGCAAACCGAGGAAAAAAAAGCGATTGCCGAAGCGGTGGCAGACTATATTCCTGATGGTTCAACAATATTTATCACCATTGGTa             <  1:3713164/150‑1 (MQ=255)
                                                                                                                                             ggTGGAGCGGGTCGGGCTTCCAGCGTCGTTAATACGGCGTTCGAGCAGCGTGAGGTTTCGCAAACCGAGGAAAAAAAAGCGATTGCCGAAGCGGTGGCAGACTATATTCCTGATGGTTCAACAATATTTATCACCATTTGTACGACTGtt     >  1:3147798/1‑150 (MQ=255)
                                                                                                                                              gtgtCGGGGGTCGGGGTTCCCGCGTCGTTAATACGTCGTTCGTTCTGCGTTTGGTTTCGAAACCCGTGTAAAAAAAAGCGATTGCCGAAGCGGTGGCAGACTATATTCCTGATGGTTCAACAATATTTATCACCATTGGTACGACTGTTg    <  1:1914877/150‑1 (MQ=255)
                                                                                                                                              gTGGAGCGGGTCGGGCTTCCAGCGTCTTTAATACGGCGTTCGAGCAGCGTGAGGTTTCGCAAACCGAGGAAAAAAAAGCGATTGCCGAAGCGGTGGCAGACTATATTCCTGATGGTTCAACAATATTTATCACCATTGGTACGACTGTTg    <  1:4193906/150‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                   
CACGACCAACTCCTCATGCTGATCGCCGAGCGTGGGTATATGAATATTGATGAGCTGGCAAATCTGCTGGATGTCTCCACGCAGACGGTCCGCCGGGATATTCGTAAATTAAGCGAGCAAGGCCTGATTACGCGCCATCACGGTGGCGCGGGTCGGGCTTCCAGCGTCGTTAATACGGCGTTCGAGCAGCGTGAGGTTTCGCAAACCGAGGAAAAAAAAGCGATTGCCGAAGCGGTGGCAGACTATATTCCTGATGGTTCAACAATATTTATCACCATTGGTACGACTGTTGAG  >  CP009273/4068062‑4068355

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑