Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 4,080,364 G→T 7.6% M1M (ATG→ATT) † yiiG → DUF3829 family lipoprotein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092734,080,3640GT 7.6% 718.5 / 8.9 211M1M (ATG→ATT) †yiiGDUF3829 family lipoprotein
Reads supporting (aligned to +/- strand):  ref base G (93/95);  new base T (19/4);  total (112/99)
Fisher's exact test for biased strand distribution p-value = 3.31e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 214 total aligned reads displayed.

TAAGTCCTGCGAAATATATTAAATTGCAGGACTTATTCATTTCGTGAATTTTATTATTTTATTTATAAACAAAATGCGTGTAGTGGCGTTTTCTTTTTTCATTTATTATGTTCACTCTCTTATCTATATATTTATAAGGCAATTAAATGAAAAGGAATTTATTATCTTCCGCTATTATAGTCGCCATCATGTCCCTCGGTCTGACGGGTTGTGATGATAAAAAAGCCGAAACAGAAACGCTCCCGCCTGCCAATAGTCAACCTGCCGCACCAGCTCCTGAAGCGAAACCTACTGAAGC  >  CP009273/4080216‑4080513
                                                                                                                                                    |                                                                                                                                                     
 aaGTCCTGCGAAATATATTAAATTGCAGGACTTATTCATTTCGTGAATTTTATTATTTTATTTATAAACAAAATGCGTGTAGTGGCGTTTTCTTTTTTCATTTATTATGTTCACTCTCTTATCTATATATTTATAAGGCAATTAAATGaa                                                                                                                                                     <  1:3525627/150‑1 (MQ=255)
  aGTCCTGCGAAATATATTAAATTGCAGGACTTATTCATTTCGTGAATTTTATTATTTTATTTATAAACAAAATGCGTGTAGTGGCGTGTTCTTTTTTAATTTATTTTTTTCAATATCTTATCTATATTTTTTTAAGGCAAttaaattaaa                                                                                                                                                    >  2:487511/1‑150 (MQ=255)
     ccTGCGAAATATATTAAATTGCAGGACTTATTCATTTCGTGAATTTTATTATTTTATTTATAAACAAAATGCGTGTAGTGGCGTTTTCTTTTTTCATTTATTATGTTCACTCTCTTATCTATATATTTATAAGGCAATTAAATGAAAAgg                                                                                                                                                 <  2:2395098/150‑1 (MQ=255)
     ccTGCGAAATATATTAAATTGCAGGACTTATTCATTTCGTGAATTTTATTATTTTATTTATAAACAAAATGCGTGTAGTGGCGTGTTGTTTTTTTATTGATTATTTTTACTCAAATATAAATTTATTTATAAAGCAAAtaaaataaaaag                                                                                                                                                 >  1:3955715/1‑148 (MQ=255)
         cGAAATATATTAAATTGCAGGACTTATTCATTTCGTGAATTTTATTATTTTATTTATAAACAAAATGCGTGTAGTGGCGTTTTCTTTTTTCATTTATTATGTTCACTCTCTTATCTATATATTTATAAGGCAATTAAATGAAAAGGAAtt                                                                                                                                             <  1:4370589/150‑1 (MQ=255)
         cGAAATATATTAAATTGCAGGACTTATTCATTTCGTGAATTTTATTATTTTATTTATAAACAAAATGCGTGTAGTGGCGTTTTCTTTTTTCATTTATTATGTTCACTCTCTTATCTATATATTTATAAGGCAATTAAATGAAAAGGAAtt                                                                                                                                             <  1:2097884/150‑1 (MQ=255)
         cGAAATATATTAAATTGCAGGACTTATTCATTTCGTGAATTTTATTATTTTATTTATAAACAAAATGCGTGTAGTGGCGTGTTATTTTTTCATTTATTATTTTAACTATCTTATCTATATATTTATAATGCAATTAAATGAAAAGTAAtt                                                                                                                                             >  2:1435009/1‑150 (MQ=255)
            aaTATATTAAATTGCAGGACTTATTCATTTCGTGAATTTTATTATTTTATTTATAAACAAAATGCGTGTAGTGGCGTTTTCTTTTTTCATTTATTATGTTCACTCTCTTATCTATATATTTATAAGGCAATTAAATGAAAAGGAATttat                                                                                                                                          <  1:4286472/150‑1 (MQ=255)
            aaTATATTAAATTGCAGGACTTATTCATTTCGTGAATTTTATTATTTTATTTATAAACAAAATGCGTGTAGTGGCGTTTTCTTTTTTCATTTATTATGTTCACTCTCTTATCTATATATTTATAAGGCAATTAAATGAAAAGGAATttat                                                                                                                                          <  1:2313992/150‑1 (MQ=255)
             atatatTAAATTGCAGGACTTATTCATTTCGTGAATTTTATTATTTTATTTATAAACAAAATGCGTGTAGTGGCGTGTTTTTTTTTTATTTATTTTTTTGACTATGTTTTATATATTTTTATAAGGGCATTAAATTAAAAAGAAAttaat                                                                                                                                         >  1:2092344/1‑148 (MQ=255)
                 atTAAATTGCAGGACTTATTCATTTCGTGAATTTTATTATTTTATTTATAAACAAAATGCGTGTAGTGGCGTTTTCTTTTTTAATTTTTTATGTTGACTCTCTTATCTATATATTTATAAGGCAAATTAATTAAAAGGAATTtattatat                                                                                                                                     >  2:2907015/1‑148 (MQ=255)
                  ttAAATTGCAGGACTTATTCATTTCGTGAATTTTATTATTTTATTTATAAACAAAATGCGTGTAGTGGCGTTTTCTTTTTTCATTTATTATGTTCACTCTCTTATCTATATATTTATAAGGCAATTAAATGAAAAGGAATTTATTATCtt                                                                                                                                    <  1:1445886/150‑1 (MQ=255)
                    aaaTTGCAGGACTTATTCATTTCGTGAATTTTATTATTTTATTTATAAACAAAATGCGTGTAGTGGCGTTTTGTTTTTTAAATTATGATTTTAAATCTCGTATAGATAAAGTTATAAGGCAAATAAAAGACAACGAATATATTATATTcc                                                                                                                                  >  2:3868916/1‑150 (MQ=255)
                     aaTTGCAGGACTTATTCATTTCGTGAATTTTATTATTTTATTTATAAACAAAATGCGTGTAGTGGCGTTTTCTTTTTTCATTTATTATGTTCACTCTCTTATCTATATATTTATAAGGCAATTAAATGAAAAGGAATTTATTATCTTCCg                                                                                                                                 <  2:767798/150‑1 (MQ=255)
                         gCAGGACTTATTCATTTCGTGAATTTTATTATTTTATTTATAAACAAAATGCGTGTAGTGGCGTTTTCTTTTTTCATTTATTATGTTCACTCTCTTATCTATATATTTATAAGGCAATTAAATGAAAAGGAATTTATTATCTTCCGCtat                                                                                                                             <  1:4104412/150‑1 (MQ=255)
                         gCAGGACTTATTCATTTCGTGAATTTTATTATTTTATTTATAAACAAAATGCGTGTAGTGGCGTTTTCTTTTTTCATTTATTATGTTCACTCTCTTATCTATATATTTATAAGGCAATTAAATGAAAAGGAATTTATTATCTTCCGCtat                                                                                                                             <  1:3298819/150‑1 (MQ=255)
                          cAGGACTTATTCATTTCGTGAATTTTATTATTTTATTTATAAACAAAATGCGTGTAGTGGCGTTTTCTTTTTTCATTTATTATGTTCACTCTCTTATCTATATATTTATAAGGCAATTAAATGAAAAGGAATTTATTATCTTCCGCtatt                                                                                                                            <  1:3753938/150‑1 (MQ=255)
                          cAGGACTTATTCATTTCGTGAATTTTATTATTTTATTTATAAACAAAATGCGTGTAGTGGCGTTTTCTTTTTTCATTTATTATGTTCACTCTCTTATCTATATATTTATAAGGCAATTAAATGAAAAGGAATTTATTATCTTCCGCtatt                                                                                                                            <  2:3150874/150‑1 (MQ=255)
                              aCTTATTCATTTCGTGAATTTTATTATTTTATTTATAAACAAAATGCGTGTAGTGGCGTTTTCTTTTTTCATTTATTATGTTCACTCTCTTATCTATATATTTATAAGGCAATTAAATGAAAAGGAATTTATTATCTTCCGCTATTATAg                                                                                                                        <  2:4430598/150‑1 (MQ=255)
                              aCTTATTCATTTCGTGAATTTTATTATTTTATTTATAAACAAAATGCGTGTAGTGGCGTTTTCTTTTTTCATTTATTATGTTCACTCTCTTATCTATATATTTATAAGGCAATTAAATGAAAAGGAATTTATTATCTTCCGCTATTATAg                                                                                                                        <  2:1691780/150‑1 (MQ=255)
                                ttATTCATTTCGTGAATTTTATTATTTTATTTATAAACAAAATGCGTGTAGTGGCGTTTTGTTTTTTGATTTTTTTTTTTGACTATGTTATCTATATATTTATTATGAAATTAAATGTAAATGAATTTATTATATTACGCGAGTATAGTc                                                                                                                      >  2:2065256/1‑150 (MQ=255)
                                ttATTCATTTCGTGAATTTTATGATTTTTTTTATAAACAATATGCGTGTAGTTGCGTTTTCTTTTTTCATTTATTATGTTCACTCTCTTATCTATATATTTATAAGGCAATTAAATGAAAAGGAATTTATTATCTTCCGCTATTATAGTc                                                                                                                      <  2:3139391/150‑1 (MQ=255)
                                           gTGAATTTTATTATTTTATTTATAAACAAAATGCGTGTAGTGGCGTTTTCTTTTTTCATTTATTATGTTCACTCTCTTATCTATATATTTATAAGGCAATTAAATGAAAAGGAATTTATTATCTTCCGCTATTATAGTCGCCATCATGTc                                                                                                           <  2:4321695/150‑1 (MQ=255)
                                            tGAATTTTATTATTTTATTTATAAACAAAATGCGTGTAGTGGCGTTTTCTTTTTTCATTTATTATGTTCACTCTCTTATCTATATATTTATAAGGCAATTAAATGAAAAGGAATTTATTATCTTCCGCTATTATAGTCGCCATCATGTcc                                                                                                          <  1:2223011/150‑1 (MQ=255)
                                               attttattattttattTATAAACAAAATGCGTGTAGTGGCGTTTTCTTTTTTCATTTATTATGTTCACTCTCTTATCTATATATTTATAAGGCAATTAAATGAAAAGGAATTTATTATCTTCCGCTATTATAGTCGCCATCATGTCCCTc                                                                                                       <  2:173334/150‑1 (MQ=255)
                                                  ttattattttattTATAAACAAAATGCGTGTAGTGGCGTGTTATTTTTTAATTTATTATGTTTACTATCTTATATAATTATTTTTAAAGCAAATAAATTAAAAGGTATTTATTATATTGCGCTAATATTGTCGCCATCAAGTcccaaggt                                                                                                    >  2:1766958/1‑145 (MQ=255)
                                                       attttatttATAAACAAAATGCGTGTATTGGCGTTTTCTTTTTTCATTTATTATGTTCACTCTCTTATCTATATATTTATAAGGCAATTAAATGAAAAGGAATTTATTATCTTCCGCTATTATAGTCGCCATCATGTCCCTCGGTCTGAc                                                                                               <  2:2731979/150‑1 (MQ=255)
                                                        ttttatttATAAACAAAATGCGTGTAGTGGCGTTTTCTTTTTTCATTTATTATGTTCACTCTCTTATCTATATATTTATAAGGCAATTAAATGAAAAGGAATTTATTATCTTCCGCTATTATAGTCGCCATCATGTCCCTCGGTCTGACg                                                                                              <  1:1034154/150‑1 (MQ=255)
                                                            atttatAAACAAAATGCGTGTAGTGGCGTTTTCTTTTTTCATTTATTATGTTCACTCTCTTATCTATATATTTATAAGGCAATTAAATGAAAAGGAATTTATTATCTTCCGCTATTATAGTCGCCATCATGTCCCTCGGTCTGACGGGtt                                                                                          <  2:723497/150‑1 (MQ=255)
                                                               tataAACAAAATGCGTGTAGTGGCGTTTTCTTTTTTCATTTATTATGTTCACTCTCTTATCTATATATTTATAAGGCAATTAAATGAAAAGGAATTTATTATCTTCCGCTATTATAGTCGCCATCATGTCCCTCGGTCTGACGGGTtgtg                                                                                       <  2:678237/150‑1 (MQ=255)
                                                                ataAACAAAATGCGTGTAGTGGCGTTTTCTTTTTTCATTTATTATGTTCACTCTCTTATCTATATATTTATAAGGCAATTAAATGAAAAGGAATTTATTATCTTCCGCTATTATAGTCGCCATCATGTCCCTCGGTCTGACGGGTTGtga                                                                                      <  1:3741036/150‑1 (MQ=255)
                                                                 taAACAAAATGCGTGTAGTGGCGTTTTCTTTTTTCATTTATTATGTTCACTCTCTTATCTATATATTTATAAGGCAATTAAATGAAAAGGAATTTATTATCTTCCGCTATTATAGTCGCCATCATGTCCCTCGGTCTGACGGGTTGtgat                                                                                     <  1:1901768/150‑1 (MQ=255)
                                                                  aaaCAAAATGCGTGTAGTGGCGTTTTATTTTTTGTTTTTTTATGTTGACTATCTTATGTATATATTTATAATGCAAATAAATGAAAAGGAAATTATTATATTACGCGAATATAGTCGCCCTCATGTCCCTCGGTCTGACGGGGTGtgttg                                                                                    >  2:2483757/1‑150 (MQ=255)
                                                                    aCAAAATGCGTGTAGTGGCGTTTTCTTTTTTCATTTATTATGTTCACTCTCTTATCTATATATTTATAAGGCAATTAAATGAAAAGGAATTTATTATCTTCCGCTATTATAGTCGCCATCATGTCCCTCGGTCTGACGGGTTGTgatgat                                                                                  <  1:4084785/150‑1 (MQ=255)
                                                                     cAAAATGCGTGTAGTGGCGTTTTCTTTTTTCATTTATTATGTTCACTCTCTTATCTATATATTTATAAGGCAATTAAATGAAAAGGAATTTATTATCTTCCGCTATTATAGTCGCCATCATGTCCCTCGGTCTGACGGGTTGTGATGATa                                                                                 <  1:2568898/150‑1 (MQ=255)
                                                                         aTGCGTGTAGTGGCGTTTTCTTTTTTAATTTATTATTTTCAATCTCTTATATATAAATTTATAATTCCATTAAAAGAAAATGTATTTTTTATATTACGCGAGTATATTAGCCAAAATTTCCCACGGTCTGTCGGGGTGTGATGTTaaaaa                                                                             >  2:3704983/1‑150 (MQ=255)
                                                                         aTGCGTGTAGTGGCGTTTTCTTTTTTAATTTATTATGTTGACTCTCTTATATATATATTTTTAATGCAAATAAATGAAAAGGAATTTATTATATTCAGCTATTATATTAGCCATCAAGTACCACGGTCTGACGGGTTTTGATGATaaaaa                                                                             >  2:3703090/1‑150 (MQ=255)
                                                                           gCGTGTAGTGGCGTTTTCTTTTTTCATTTATTATGTTCACTCTCTTATCTATATATTTATAAGGCAATTAAATGAAAAGGAATTTATTATCTTCCGCTATTATAGTCGCCATCATGTCCCTCGGTCTGACGGGTTGTGATGATAAAAAAg                                                                           <  2:4239666/150‑1 (MQ=255)
                                                                             gtgtAGTGGCGTTTTCTTTTTTCATTTATTATGTTCACTCTCTTATCTATATATTTATAAGGCAATTAAATGAAAAGGAATTTATTATCTTCCGCTATTATAGTCGCCATCATGTCCCTCGGTCTGACGGGTTGTGATGATAAAAAAGcc                                                                         <  1:3049000/150‑1 (MQ=255)
                                                                                 aGTGGCGTTTTCTTTTTTCATTTATTATGTTCACTCTCTTATCTATATATTTATAAGGCAATTAAATGAAAAGGAATTTATTATCTTCCGCTATTATAGTCGCCATCATGTCCCTCGGTCTGACGGGTTGTGATGATAAAAAAGCCGaaa                                                                     <  2:1796518/150‑1 (MQ=255)
                                                                                   tGGCGTTTTCTTTTTTCATTTATTATGTTCACTCTCTTATCTATATATTTATAAGGCAATTAAATGAAAAGGAATTTATTATCTTCCGCTATTATAGTCGCCATCATGTCCCTCGGTCTGACGGGTTGTGATGATAAAAAAGCCGAAACa                                                                   >  2:1073168/1‑150 (MQ=255)
                                                                                       gTTTTCTTTTTTCATTTATTATGTTCACTCTCTTATCTATATATTTATAAGGCAATTAAATGAAAAGGAATTTATTATCTTCCGCTATTATAGTCGCCATCATGTCCCTCGGTCTGACGGGTTGTGATGATAAAAAAGCCGAAACAGaaa                                                               >  2:2600590/1‑150 (MQ=255)
                                                                                        ttttCTTTTTTCATTTATTATGTTCACTCTCTTATCTATATATTTATAAGGCAATTAAATGAAAAGGAATTTATTATCTTCCGCTATTATAGTCGCCATCATGTCCCTCGGTCTGACGGGTTGTGATGATAAAAAAGCCGAAACAGAAAc                                                              >  1:4017029/1‑150 (MQ=255)
                                                                                         tttCTTTTTTCATTTATTATGTTCACTCTCTTATCTATATATTTATAAGGCAATTAAATGAAAAGGAATTTATTATCTTCCGCTATTATAGTCGCCATCATGTCCCTCGGTCTGACGGGTTGTGATGATAAAAAAGCCGAAACAGAAACg                                                             <  2:3054341/150‑1 (MQ=255)
                                                                                            cTTTTTTCATTTATTATGTTCACTCTCTTATCTATATATTTATAAGGCAATTAAATGAAAAGGAATTTATTATCTTCCGCTATTATAGTCGCCATCATGTCCCTCGGTCTGACGGGTTGTGATGATAAAAAAGCCGAAACAGAAACGCTc                                                          >  2:575481/1‑150 (MQ=255)
                                                                                            cTTTTTTCATTTATTATGTTCACTCTCTTATCTATATATTTATAAGGCAATTAAATGAAAAGGAATTTATTATCTTCCGCTATTATAGTCGCCATCATGTCCCTCGGTCTGACGGGTTGTGATGATAAAAAAGCCGAAACAGAAACGCTc                                                          >  2:575455/1‑150 (MQ=255)
                                                                                             ttttttCATTTATTATGTTCACTCTCTTATCTATATATTTATAAGGCAATTAAATGAAAAGGAATTTATTATCTTCCGCTATTATAGTCGCCATCATGTCCCTCGGTCTGACGGGTTGTGATGATAAAAAAGCCGAAACAGAAACGCTcc                                                         >  2:2654459/1‑150 (MQ=255)
                                                                                             ttttttCATTTATTATGTTCACTCTCTTATCTATATATTTATAAGGCAATTAAATGAAAAGGAATTTATTATCTTCCGCTATTATAGTCGCCATCATGTCCCTCGGTCTGACGGGTTGTGATGATAAAAAAGCCGAAACAGAAACGCTcc                                                         >  2:385748/1‑150 (MQ=255)
                                                                                              tttttAATTTATTATGTTCACTCTCTTATCTATATATTTATAAGGCAATTAAATGAAAAGGAATTTATTATCTTCCGCTATTATAGTCGCCATCATGTCCCTCGGTCTGACGGGTTGTGATGATAAAAAAGCCGAAACAGAAACGCTccc                                                        <  2:1636842/150‑1 (MQ=255)
                                                                                                tttCATTTATTATGTTCACTCTCTTATCTATATATTTATAAGGCAATTAAATGAAAAGGAATTTATTATCTTCCGCTATTATAGTCGCCATCATGTCCCTCGGTCTGACGGGTTGTGATGATAAAAAAGCCGAAACAGAAACGCTCCCGc                                                      >  2:1262551/1‑150 (MQ=255)
                                                                                                   cATTTATTATGTTCACTCTCTTATCTATATATTTATAAGGCAATTAAATGAAAAGGAATTTATTATCTTCCGCTATTATAGTCGCCATCATGTCCCTCGGTCTGACGGGTTGTGATGATAAAAAAGCCGAAACAGAAACGCTCCCGCCTg                                                   >  1:3401541/1‑150 (MQ=255)
                                                                                                     tttATTATGTTCACTCTCTTATCTATATATTTATAAGGCAATAAAATGAAAAGGAATTTATTATCTTCCGCTATTATAGTCGCCATCATGTCCCTCGGTCTGACGGGTTGTGATGATAAAAAAGCCGAAACAGAAACGCTCCCGCCTGcc                                                 >  1:3571699/1‑150 (MQ=255)
                                                                                                      ttattaTGTTCACTCTCTTATCTATATATTTATAAGGCAATTAAATGAAAAGGAATTTATTATCTTCCGCTATTATAGTCGCCATCATGTCCCTCGGTCTGACGGGTTGTGATGATAAAAAAGCCGAAACAGAAACGCTCCCGCCTGCCa                                                >  1:661569/1‑150 (MQ=255)
                                                                                                         ttatGTTCACTCTCTTATCTATATATTTATAAGGCAATTAAATGAAAAGGAATTTATTATCTTCCGCTATTATAGTCGCCATCATGTCCCTCGGTCTGACGGGTTGTGATGATAAAAAAGCCGAAACAGAAACGCTCCCGCCTGCCAATa                                             <  2:3183488/150‑1 (MQ=255)
                                                                                                           atGTTCACTCTCTTATCTATATATTTATAAGGCAATTAAATGAAAAGGAATTTATTATCTTCCGCTATTATAGTCGCCATCATGTCCCTCGGTCTGACGGGTTGTGATGATAAAAAAGCCGAAACAGAAACGCTCCCGCCTGCCAATAGt                                           >  2:1269731/1‑150 (MQ=255)
                                                                                                           atGTTCACTCTCTTATCTATATATTTATAAGGCAATTAAATGAAAAGGAATTTATTATCTTCCGCTATTATAGTCGCCATCATGTCCCTCGGTCTGACGGGTTGTGATGATAAAAAAGCCGAAACAGAAACGCTCCCGCCTGCCAATAGt                                           >  2:3555716/1‑150 (MQ=255)
                                                                                                            tGTTCACTCTCTTATCTATATATTTATAAGGCAATTAAATTAAAAGGAATTTATTATCTTCCGCTATTATAGTCGCCATCATGTCCCTCGGTCTGCCGGGTTGTGATTATAAAAAAGCCGAAACAGAAACGCTCCCGCCTGCCAATAGTc                                          <  1:868042/150‑1 (MQ=255)
                                                                                                            tGTTCACTCTCTTATCTATATATTTATAAGGCAATTAAATGAAAAGGAATTTATTATCTTCCGCTATTATAGTCGCCATCATGTCCCTCGGTCTGACGGGTTGTGATGATAAAAAAGCCGAAACAGAAACGCTCCCGCCTGCCAATAgac                                          >  2:4511823/1‑148 (MQ=255)
                                                                                                            tGTTCACTCTCTTATCTATATATTTATAAGGCAATTAAATGAAAAGGAATTTATTATCTTCCGCTATTATAGTCGCCATCATGTCCCTCGGTCTGACGGGTTGTGATGATAAAAAAGCCGAAACAGAAACGCTCCCGCCTGCCAATAGTc                                          >  1:1893918/1‑150 (MQ=255)
                                                                                                            tGTTCACTCTCTTATCTATATATTTATAAGGCAATTAAATGAAAAGGAATTTATTATCTTACGCTATTATAGTCGCAATCATGTCCCTCGGTCTGACGGGTTGTGATGATAAAAAAGCCGAAACAGAAAAGCTCCCGCCTGCCAATAGTc                                          >  2:4511806/1‑150 (MQ=255)
                                                                                                             gTTCACTCTCTTATCTATATATTTATAAGGCAATTAAATGAAAAGGAATTTATTATCTTCCGCTATTATAGTCGCCATCATGTCCCTCGGTCTGACGGGTTGTGATGATAAAAAAGCCGAAACAGAAACGCTCCCGCCTGCCAATAGTCa                                         >  1:1040110/1‑150 (MQ=255)
                                                                                                              ttCACTCTCTTATCTATATATTTATAAGGCAATTAAATGAAAAGGAATTTATTATCTTCCGCTATTATAGTCGCCATCATGTCCCTCGGTCTGACGGGTTGTGATGATAAAAAAGCCGAAACAGAAACGCTCCCGCCTGCCAATAGTCaa                                        >  1:3323409/1‑150 (MQ=255)
                                                                                                                cACTCTCTTATCTATATATTTATAAGGCAATTAAATGAAAAGGAATTTATTATCTTCCGCTATTATAGTCGCCATCATGTCCCTCGGTCTGACGGGTTGTGATGATAAAAAAGCCGAAACAGAAACGCTCCCGCCTGCCAATAGTCAAcc                                      >  2:2577961/1‑150 (MQ=255)
                                                                                                                cACTCTCTTATCTATATATTTATAAGGCAATTAAATGAAAAGGAATTTATTATCTTCCGCTATTATAGTCGCCATCATGTCCCTCGGTCTGACGGGTTGTGATGATAAAAAAGCCGAAACAGAAACGCTCCCGCCTGCCAATAGTCAAcc                                      >  1:1194892/1‑150 (MQ=255)
                                                                                                                   tctctTATCTATATATTTATAAGGCAATTAAATGAAAAGGAATTTATTATCTTCCGCTATTATAGTCGCCATCATGTCCCTCGGTCTGACGGGTTGTGATGATAAAAAAGCCGAAACAGAAACGCTCCCGCCTGCCAATAGTCAACCTGc                                   >  1:3591060/1‑150 (MQ=255)
                                                                                                                    ctctTATCTATATATTTATAAGGCAATTAATTTAAAAGTAATTTATTATCTTCCGCTTTTTTAGTCGCCATCATGTCCCTCGGTCTGACGGGTTGTGATGATAAAAAAGCCGAAACAGAAACGCTCCCGCCTGCCAATAGTCAACCTGcc                                  <  1:2349460/150‑1 (MQ=255)
                                                                                                                    ctctTATCTATATATTTATAAGGCAATTAAATGAAAAGGAATTTATTATCTTCCGCTATTATAGTCGCCATCATGCCCCTCGGTCTGACGGGTTGTGATGATAAAAAAGCCGAAACAGAAACGCTCCCGCCTGCCAATAGTCAACCTGcc                                  <  2:223626/150‑1 (MQ=255)
                                                                                                                     tctTATCTATATATTTATAAGGCAATTAAATGAAAAGGAATTTATTATCTTCCGCTATTATAGTCGCCATCATGTCCCTCGGTCTGACGGGTTGTGATGATAAAAAAGCCGAAACAGAAACGCTCCCGCCTGCCAATAGTCAACCTGCCg                                 >  2:3246469/1‑150 (MQ=255)
                                                                                                                      ctTATCTATATATTTATAAGGCAATTAAATGAAAAGGAATTTATTATCTTCCGCTATTATAGTCGCCATCATGTCCCTCGGTCTGACGGGTTGTGATGATAAAAAAGCCGAAACAGAAACGCTCCCGCCTGCCAATAGTCAACCTGCCGc                                >  2:2435343/1‑150 (MQ=255)
                                                                                                                         aTCTATATATTTATAAGGCAATTAAATTAAAAGTAATTTATTATCTTCCGCTATTATAGTCGCCATCATGTCCCTCGGTCTGACGGGTTGTGATGATAAAAAAGCCGAAACAGAAACGCTCCCGCCTGCCAATAGTCAACCTGCCGCAcc                             <  1:4264005/150‑1 (MQ=255)
                                                                                                                         aTCTATATATTTATAAGGCAATTAAATGAAAAGGAATTTATTATCTTCCGCTATTATAGTCGCCATCATGTCCCTCGGTCTGACGGGTTGTGATGATAAAAAAGCCGAAACAGAAACGCTCCCGCCTGCCAATAGTCAACCTGCCGCAcc                             >  2:2540728/1‑150 (MQ=255)
                                                                                                                         aTCTATATATTTATAAGGCAATTAAATGAAAAGGAATTTATTATCTTCCGCTATTATAGTCGCCATCATGTCCCTCGGTCTGACGGGTTGTGATGATAAAAAAGCCGAAACAGAAACGCTCCCGCCTGCCAATAGTCAACCTGCCGCAcc                             >  2:655594/1‑150 (MQ=255)
                                                                                                                          tCTATATATTTATAAGGCAATTAAATGAAAAGGAATTTATTATCTTCCGCTATTATAGTCGCCATCATGTCCCTCGGTCTGACGGGTTGTGATGATAAAAAAGCCGAAACAGAAACGCTCCCGCCTGCCAATAGTCAACCTGCCGCACCa                            >  2:2803861/1‑150 (MQ=255)
                                                                                                                          tCTATATATTTATAAGGCAATTAAATGAAAAGGAATTTATTATCTTCCGCTATTATAGTCGCCATCATGTCCCTCGGTCTGACGGGTTGTGATGATAAAAAAGCCGAAACAGAAACGCTCCCGCCTGCCAATAGTCAACCTGCCGCACCa                            <  1:2876441/150‑1 (MQ=255)
                                                                                                                          tCTATATATTTATAAGGCAATTAAATGAAAAGGAATTTATTATCTTCCGCTATTATAGTCGCCATCATGTCCCTCGGTCTGACGGGTTGTGATGATAAAAAAGCCGAAACAGAAACGCTCCCGCCTGCCAATAGTCAACCTGCCGCACCa                            <  1:3693828/150‑1 (MQ=255)
                                                                                                                            tatataTTTATAAGGCAATTAAATGAAAAGGAATTTATTATCTTCCGCTATTATAGTCGCCATCATGTCCCTCGGTCTGACGGGTTGTGATGATAAAAAAGCCGAAACAGAAACGCTCCCGCCTGCCAATAGTCAACCTGCCGCACCAGc                          >  2:2923945/1‑150 (MQ=255)
                                                                                                                             atatatTTATAAGGCAATTAAATGAAAAGGAATTTATTATCTTCCGCTATTATAGTCGCCATCATGTCCCTCGGTCTGACGGGTTGTGATGATAAAAAAGCCGAAACAGAAACGCTCCCGCCTGCCAATAGTCAACCTGCCGCACCAGCt                         >  2:820993/1‑150 (MQ=255)
                                                                                                                                tatTTATAAGGCAATTAAATGAAAAGGAATTTATTATCTTCCGCTATTATAGTCGCCATCATGTCCCTCGGTCTGACGGGTTGTGATGATAAAAAAGCCGAAACAGAAACGCTCCCGCCTGCCAATAGTCAACCTGCCGCACCAGCTCCt                      >  1:254035/1‑150 (MQ=255)
                                                                                                                                 atTTATAAGGCAATTAAATGAAAAGGAATTTATTATCTTCCGCTATTATAGTCGCCATCATGTCCCTCGGTCTGACGGGTTGTGATGATAAAAAAGCCGAAACAGAAACGCTCCCGCCTGCCAATAGTCAACCTGCCGCACCAGCTCCTg                     >  2:2046547/1‑150 (MQ=255)
                                                                                                                                  tttATAAGGCAATTAAATGAAAAGGAATTTATTATCTTCCGCTATTATAGTCGCCATCATGTCCCTCGGTCTGACGGGTTGTGATGATAAAAAAGCCGAAACAGAAACGCTCCCGCCTGCCAATAGTCAACCTGCCGCACCAGCTCCTGa                    <  2:3963109/150‑1 (MQ=255)
                                                                                                                                    tataAGGCAATTAAATGAAAAGGAATTTATTATCTTCCGCTATTATAGTCGCCATCATGTCCCTCGGTCTGACGGGTTGTGATGATAAAAAAGCCGAAACAGAAACGCTCCCGCCTGCCAATAGTCAACCTGCCGCACCAGCTCCTGAAg                  >  2:3353019/1‑150 (MQ=255)
                                                                                                                                    tataAGGCAATTAAATGAAAAGGAATTTATTATCTTCCGCTATTATAGTCGCCATCATGTCCCTCGGTCTGACGGGTTGTGATGATAAAAAAGCCGAAACAGAAACGCTCCCGCCTGCCAATAGTCAACCTGCCGCACCAGCTCCTGAAg                  <  1:2482071/150‑1 (MQ=255)
                                                                                                                                     ataAGGCAATTAAATGAAAAGGAATTTATTATCTTCCGCTATTATAGTCGCCATCATGTCCCTCGGTCTGACGGGTTGTGATGATAAAAAAGCCGAAACAGAAACGCTCCCGCCTGCCAATAGTCAACCTGCCGCACCAGCTCCTGAAGc                 <  1:1726435/150‑1 (MQ=255)
                                                                                                                                     ataAGGCAATTAAATGAAAAGGAATTTATTATCTTCCGCTATTATAGTCGCCATCATGTCCCTCGGTCTGACGGGTTGTGATGATAAAAAAGCCGAAACAGAAACGCTCCCGCCTGCCAATAGTCAACCTGCCGCACCAGCTCCTGAAGc                 <  1:4004857/150‑1 (MQ=255)
                                                                                                                                       aaGGCAATTAAATGAAAAGGAATTTATTATCTTCCGCTATTATAGTCGCCATCATGTCCCTCGGTCTGACGGGTTGTGATGATAAAAAAGCCGAAACAGAAACGCTCCCGCCTGCCAATAGTCAACCTGCCGCACCAGCTCCTGAAGCGa               >  2:3497428/1‑150 (MQ=255)
                                                                                                                                       aaGGCAATTAAATGAAAAGGAATTTATTATCTTCCGCTATTATAGTCGCCATCATGTCCCTCGGTCTGACGGGTTGTGATGATAAAAAAGCCGAAACAGAAACGCTCCCGCCTGCCAATAGTCAACCTGCCGCACCAGCTCCTGAAGCGa               >  1:1484943/1‑150 (MQ=255)
                                                                                                                                       aaGGCAATTAAATGAAAAGGAATTTATTATCTTCCGCTATTATAGTCGCCATCATGTCCCTCGGTCTGACGGGTTGTGATGATAAAAAAGCCGAAACAGAAACGCTCCCGCCTGCCAATAGTCAACCTGCCGCACCAGCTCCTGAAGCGa               >  2:1553595/1‑150 (MQ=255)
                                                                                                                                        aGGCAATTAAATGAAAAGGAATTTATTATCTTCCGCTATTATAGTCGCCATCATGTCCCTCGGTCTGACGGGTTGTGATGATAAAAAAGCCGAAACAGAAACGCTCCCGCCTGCCAATAGTCAACCTGCCGCACCAGCTCCTGAAGCGaa              <  2:1041829/150‑1 (MQ=255)
                                                                                                                                          gCAATTAAATGAAAAGGAATTTATTATCTTCCGCTATTATAGTCGCCATCATGTCCCTCGGTCTGACGGGTTGTGATGATAAAAAAGCCGAAACAGAAACGCTCCCGCCTGCCAATAGTCAACCTGCCGCACCAGCTCCTGAAGCGAAAc            >  1:2239533/1‑150 (MQ=255)
                                                                                                                                          gCAATTAAATGAAAAGGAATTTATTATCTTCCGCTATTATAGTCGCCATCATGTCCCTCGGTCTGACGGGTTGTGATGATAAAAAAGCCGAAACAGAAACGCTCCCGCCTGCCAATAGTCAACCTGCCGCACCAGCTCCTGAAGCGAAAc            >  2:2816184/1‑150 (MQ=255)
                                                                                                                                           cAATTAAATGAAAAGGAATTTATTATCTTCCGCTATTATAGTCGCCATCATGTCCCTCGGTCTGACGGGTTGTGATGATAAAAAAGCCGAAACAGAAACGCTCCCGCCTGCCAATAGTCAACCTGCCGCACCAGCTCCTGAAGCGAAAcc           >  2:3611217/1‑150 (MQ=255)
                                                                                                                                           cAATTAAATGAAAAGGAATTTATTATCTTCCGCTATTATAGTCGCCATCATGTCCCTCGGTCTGACGGGTTGTGATGATAAAAAAGCCGAAACAGAAACGCTCCCGCCTGCCAATAGTCAACCTGCCGCACCAGCTCCTGAAGCGAAAcc           >  2:1945179/1‑150 (MQ=255)
                                                                                                                                            aaTTAAATGAAAAGGAATTTATTATCTTCCGCTATTATAGTCGCCATCATGTCCCTCGGTCTGACGGGTTGTGATGATAAAAAAGCCGAAACAGAAACGCTCCCGCCTGCCAATAGTCAACCTGCCGCACCAGCTCCTGAAGCGAAACCt          >  2:163254/1‑150 (MQ=255)
                                                                                                                                            aaTTAAATGAAAAGGAATTTATTATCTTCCGCTATTATAGTCGCCATCATGTCCCTCGGTCTGACGGGTTGTGATGATAAAAAAGCCGAAACAGAAACGCTCCCGCCTGCCAATAGTCAACCTGCCGCACCAGCTCCTGAAGCGAAACCt          >  1:2945575/1‑150 (MQ=255)
                                                                                                                                             aTTAAATGAAAAGGAATTTATTATCTTCCGCTATTATAGTCGCCATCATGTCCCTCGGTCTGACGGGTTGTGATGATAAAAAAGCCGAAACAGAAACGCTCCCGCCTGCCAATAGTCAACCTGCCGCACCAGCTCCTGAAGCGAAACCTa         >  2:29503/1‑150 (MQ=255)
                                                                                                                                               tAAATGAAAAGGAATTTATTATCTTCCGCTATTATAGTCGCCATCATGTCCCTCGGTCTGACGGGTTGTGATGATAAAAAAGCCGAAACAGAAACGCTCCCGCCTGCCAATAGTCAACCTGCCGCACCAGCTCCTGAAGCGAAACCTACt       >  2:113873/1‑150 (MQ=255)
                                                                                                                                               tAAATGAAAAGGAATTTATTATCTTCCGCTATTATAGTCGCCATCATGTCCCTCGGTCTGACGGGTTGTGATGATAAAAAAGCCGAAACAGAAACGCTCCCGCCTGCCAATAGTCAACCTGCCGCACCAGCTCCTGAAGCGAAACCTACt       <  2:3822529/150‑1 (MQ=255)
                                                                                                                                               tAAATGAAAAGGAATTTATTATCTTCCGCTATTATAGTCGCCATCATGTCCCTCGGTCTGACGGGTTGTGATGATAAAAAAGCCGAAACAGAAACGCTCCCGCCTGCCAATAGTCAACCTGCCGCACCAGCTCCTGAAGCGAAACCTACt       >  2:1755885/1‑150 (MQ=255)
                                                                                                                                                aaaTGAAAAGGAATTTATTATCTTCCGCTATTATAGTCGCCATCATGTCCCTCGGTCTGACGGGTTGTGATGATAAAAAAGCCGAAACAGAAACGCTCCCGCCTGCCAATAGTCAACCTGCCGCACCAGCTCCTGAAGCGAAACCTACTg      >  1:952928/1‑150 (MQ=255)
                                                                                                                                                   tGAAAAGGAATTTATTATCTTCCGCTATTATAGTCGCCATCATGTCCCTCGGTCTGACGGGTTGTGATGATAAAAAAGCCGAAACAGAAACGCTCCCGCCTGCCAATAGTCAACCTGCCGCACCAGCTCCTGAAGCGAAACCTACTGAAg   >  1:452525/1‑150 (MQ=255)
                                                                                                                                                    |                                                                                                                                                     
TAAGTCCTGCGAAATATATTAAATTGCAGGACTTATTCATTTCGTGAATTTTATTATTTTATTTATAAACAAAATGCGTGTAGTGGCGTTTTCTTTTTTCATTTATTATGTTCACTCTCTTATCTATATATTTATAAGGCAATTAAATGAAAAGGAATTTATTATCTTCCGCTATTATAGTCGCCATCATGTCCCTCGGTCTGACGGGTTGTGATGATAAAAAAGCCGAAACAGAAACGCTCCCGCCTGCCAATAGTCAACCTGCCGCACCAGCTCCTGAAGCGAAACCTACTGAAGC  >  CP009273/4080216‑4080513

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑