Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 4,273,297 A→T 8.3% W512R (TGG→AGG)  actP ← acetate transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092734,273,2970AT 8.3% 795.7 / 44.4 242W512R (TGG→AGG) actPacetate transporter
Reads supporting (aligned to +/- strand):  ref base A (108/113);  new base T (10/11);  total (118/124)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 245 total aligned reads displayed.

CCCCGGTCAGACGACCGGGGAAGGGGAGAGATTAATGCGCGCGGCCTTGCTCAACGCCAAAGCCGGTCTGGGAGCGGATAAACTGCGCGCGGAACAGTTCACGCTCACGCGCGCCTTCCGCTGAGTTATCGGTTGCCGAGAAGAACCAGATGCCGAGGAATGCCACGGTGATAGAGAACAGCGCCGGGTATTCATACGGGAAGATGGCTTTTTCGTGACCAAGGATCTGTACCCAAATCGTCGGGCCGAGGATCATCAGTACTACTGCGGTAATCAGCCCCAGCCAGCCACCCATCA  >  CP009273/4273150‑4273446
                                                                                                                                                   |                                                                                                                                                     
ccccGGTCAGACGACCGGGGAAGGGGAGAGATTAATGCGCGCGGCCTTGCTCAACGCCAAAGCCGGTCTGGGAGCGGATAAACTGCGCGCGGAACAGTTCACGCTCACGCGCGCCTTCCGCTGAGTTATCGGTTGCCGAGAAGAACCAGa                                                                                                                                                     <  2:924765/150‑1 (MQ=255)
  ccGGTCAGACGACCGGGGAAGGGGAGAGATTAATGCGCGCGGCCTTGCTCAACGCCAAAGCCGGTCTGGGAGCGGATAAACTGCGCGCGGAACAGTTCACGCTCACGCGCGCCTTCCGCTGAGTTATCGGTTGCCGAGAAGAACCTGATg                                                                                                                                                   <  2:4221623/150‑1 (MQ=255)
    ggTCAGACGACCGGGGAAGGGGAGAGATTAATGCGCGCGGCCTTGCTCAACGCCAAAGCCGGTCTGGGAGCGGATAAACTGCGCGCGGAACAGTTCACGCTCACGCGCGCCTTCCGCTGAGTTATCGGTTGCCGAGAAGAACCAGATGcc                                                                                                                                                 <  2:1788120/150‑1 (MQ=255)
      tCAGACGACCGGGGAAGGGGAGAGATTAATGCGCGCGGCCGTGCTCAACGCCACAGCCGGTCTGGGAGCGGATAAACTGCGCGCGGAACAGGTCACGCTCACGCGCGCCTTCCGCTGAGTTATCGGTTGCCGAGAAGAACCAGATGCCGa                                                                                                                                               <  2:1958711/150‑1 (MQ=255)
        aGACGACCGGGGAAGGGGAGAGATTAATGCGCGCGGCCTTGCTCAACGCCAAAGCCGGTCTGGGAGCGGATAAACTGCGCGCGGAACAGTTCACGCTCACGCGCGCCTTCCGCTGAGTTATCGGTTGCCGAGAAGAACCAGATGCCGAgg                                                                                                                                             <  2:2524146/150‑1 (MQ=255)
            gacCGGGGAAGGGGAGAGATTAATGCGCGCGGCCTTGCTCAACGCCAAAGCCGGTCTGGGAGCGGATAAACTGCGCGCGGAACAGTTCACGCTCACGCGCGCCTTCCGCTGAGTTATCGGTTGCCGAGAAGAACCTGATGCCGAGGAATg                                                                                                                                         >  2:3876797/1‑150 (MQ=255)
             acCGGGGAAGGGGAGAGATTAATGCGCGCGGCCTTGCTCAACGCCAAAGCCGGTCTGGGAGCGGATAAACTGCGCGCGGAACAGTTCACGCTCACGCGCGCCTTCCGCTGAGTTATCGGTTGCCGAGAAGAACCAGATGCCGAGGAATGc                                                                                                                                        >  2:3323024/1‑150 (MQ=255)
                 gggAAGGGGAGAGATTAATGCGCGCGGCCTTGCTCAACGCCAAAGCCGGTCTGGGAGCGGATAAACTGCGCGCGGAACAGTTCACGCTCACGCGCGCCTTCCGCTGAGTTATCGGTTGCCGAGAAGAACCAGATGCCGAGGAATGCCACg                                                                                                                                    <  1:1574720/150‑1 (MQ=255)
                 gggAAGGGGAGAGATTAATGCGCGCGGCCTTGCTCAACGCCAAAGCCGGTCTGGGAGCGGATAAACTGCGCGCGGAACAGTTCACGCTCACGCGCGCCTTCCGCTGAGTTATCGGTTGCCGAGAAGAACCAGATGCCGAGGAATGCCACg                                                                                                                                    <  2:1368495/150‑1 (MQ=255)
                   gAAGGGGAGAGATTAATGCGCGCGGCCTTGCTCAACGCCAAAGCCGGTCTGGGAGCGGATAAACTGCGCGCGGAACAGTTCACGCTCACGCGCGCCTTCCGCTGAGTTATCGGTTGCCGAGAAGAACCAGATGCCGAGGAATGCCACGGt                                                                                                                                  <  1:2797468/150‑1 (MQ=255)
                      ggggAGAGATTAATGCGCGCGGCCTTGCTCAACGCCAAAGCCGGTCTGGGAGCGGATAAACTGCGCGCGGAACAGTTCACGCTCACGCGCGCCTTCCGCTGAGTTATCGGTTGCCGAGAAGAACCAGATGCCGAGGAATGCCACGGTGAt                                                                                                                               >  1:1852230/1‑150 (MQ=255)
                       gggAGAGATTAATGCGCGCGGCCTTGCTCAACGCCAAAGCCGGTCTGGGAGCGGATAAACTGCGCGCGGAACAGTTCACGCTCACGCGCGCCTTCCGCTGAGTTATCGGTTGCCGAGAAGAACCAGATGGCGAGGAATGCCACGGTGATa                                                                                                                              >  2:825024/1‑150 (MQ=255)
                       gggAGAGATTAATGCGCGCGGCCTTGCTCAACGCCAAAGCCGGTCTGGGAGCGGATAAACTGCGCGCGGAACAGTTCACGCTCACGCGCGCCTTCCGCTGAGTTATCGGTTGCCGAGAAGAACCAGATGGCGAGGAATGCCACGGTGATa                                                                                                                              >  2:1350113/1‑150 (MQ=255)
                       gggAGAGATTAATGCGCGCGGCCTTGCTCAACGCCAAAGCCGGTCTGGGAGCGGATAAACTGCGCGCGGAACAGTTCACGCTCACGCGCGCCTTCCGCTGAGTTATCGGTTGCCGAGAAGAACCAGATGCCGAGGAATGCCACGGTGATa                                                                                                                              <  1:1233149/150‑1 (MQ=255)
                       gggAGAGATTAATGCGCGCGGCCTTGCTCAACGCCAAAGCCGGTCTGGGAGCGGATAAACTGCGCGCGGAACAGTTCACGCTCACGCGCGCCTTCCGCTGAGTTATCGGTTGCCGAGAAGAACCAGATGCCGAGGAATGCCACGGTGATa                                                                                                                              >  1:4366123/1‑150 (MQ=255)
                        ggAGAGATTAATGCGCGCGGCCTTGCTCAACGCCAAAGCCGGTCTGGGAGCGGATAAACTGCGCGCGGAACAGTTCACGCTCACGCGCGCCTTCCGCTGAGTTATCGGTTGCCGAGAAGAACCTGATGCCGAGGAATGCCACGGTGATag                                                                                                                             >  2:2598303/1‑150 (MQ=255)
                         gagagaTTAATGCGCGCGGCCTTGCTCAACGCCAAAGCCGGTCTGGGAGCGGATAAACTGCGCGCGGAACAGTTCACGCTCACGCGCGCCTTCCGCTGAGTTATCGGTTGCCGAGAAGAACCAGATGCCGAGGAATGCCACGGTGATaga                                                                                                                            <  1:3377794/150‑1 (MQ=255)
                         gagagaTTAATGCGCGCGGCCTTGCTCAACGCCAAAGCCGGTCTGGGAGCGGATAAACTGCGCGCGGAACAGTTCACGCTCACGCGCGCCTTCCGCTGAGTTATCGGTTGCCGAGAAGAACCAGATGCCGAGGAATGCCACGGTGATaga                                                                                                                            <  1:1094660/150‑1 (MQ=255)
                           gagaTTAATGCGCGCGGCCTTGCTCAACGCCAAAGCCGGTCTGGGAGCGGATAAACTGCGCGCGGAACAGTTCACGCTCACGCGCGCCTTCCGCTGAGTTATCGGTTGCCGAGAAGAACCAGATGCCGAGGAATGCCACGGTGATAgaga                                                                                                                          >  2:925449/1‑150 (MQ=255)
                            agaTTAATGCGCGCGGCCTTGCTCAACGCCAAAGCCGGTCTGGGAGCGGATAAACTGCGCGCGGAACAGTTCACGCTCACGCGCGCCTTCCGCTGAGTTATCGGTTGCCGAGAAGAACCAGATGCCGAGGAATGCCACGGTGATAGAGaa                                                                                                                         <  2:1846718/150‑1 (MQ=255)
                                tAATGCGCGCGGCCTTGCTCAACGCCAAAGCCGGTCTGGGAGCGGATAAACTGCGCGCGGAACAGTTCACGCTCACGCGCGCCTTCCGCTGAGTTATCGGTTGCCGAGAAGAACCAGATGCCGAGGAATGCCACGGTGATAGAGAACAgc                                                                                                                     >  1:1904634/1‑150 (MQ=255)
                                tAATGCGCGCGGCCTTGCTCAACGCCAAAGCCGGTCTGGGAGCGGATAAACTGCGCGCGGAACAGTTCACGCTCACGCGCGCCTTCCGCTGAGTTATCGGTTGCCGAGAAGAACCAGATGCCGAGGAATGCCACGGTGATAGAGAACAgc                                                                                                                     <  1:651527/150‑1 (MQ=255)
                                 aaTGCGCGCGGCCTTGCTCAACGCCAAAGCCGGTCTGGGAGCGGATAAACTGCGCGCGGAACAGTTCACGCTCACGCGCGCCTTCCGCTGAGTTATCGGTTGCCGAGAAGAACCAGATGCCGAGGAATGCCACGGTGATAGAGAACAgcg                                                                                                                    >  1:3830395/1‑150 (MQ=255)
                                    gcgcgcGGCCTTGCTCAACGCCAAAGCCGGTCTGGGAGCGGATAAACTGCGCGCGGAACAGTTCACGCTCACGCGCGCCTTCCGCTGAGTTATCGGTTGCCGAGAAGAACCAGATGCCGAGGAATGCCACGGTGATAGAGAACAGCGCCg                                                                                                                 <  1:1709001/150‑1 (MQ=255)
                                       cgcgGCCTTGCTCAACGCCAAAGCCGGTCTGGGAGCGGATAAACTGCGCGCGGAACAGTTCACGCTCACGCGCGCCTTCCGCTGAGTTATCGGTTGCCGAGAAGAACCAGATGCCGAGGAATGCCACGGTGATAGAGAACAGCGCCGGGt                                                                                                              <  2:4496071/150‑1 (MQ=255)
                                            ccTTGCTCAACGCCAAAGCCGGTCTGGGAGCGGATAAACTGCGCGCGGAACAGTTCACGCTCACGCGCGCCTTCCGCTGAGTTATCGGTTGCCGAGAAGAACCAGATGCCGAGGAATGCCACGGTGATAGAGAACAGCGCCGGGTCTTCa                                                                                                         >  2:2029143/1‑150 (MQ=255)
                                            ccTTGCTCAACGCCAAAGCCGGTCTGGGAGCGGATAAACTGCGCGCGGAACAGTTCACGCTCACGCGCGCCTTCCGCTGAGTTATCGGTTGCCGAGAAGAACCAGATGCCGAGGAATGCCACGGTGATAGAGAACAGCGCCGGGTATTCa                                                                                                         >  1:103906/1‑150 (MQ=255)
                                            ccTTGCTCAACGCCAAAGCCGGTCTGGGAGCGGATAAACTGCGCGCGGAACAGTTCACGCTCACGCGCGCCTTCCGCTGAGTTATCGGTTGCCGAGAAGAACCAGATGCCGAGGAATGCCACGGTGATAGAGAACAGCGCCGGGTATTCa                                                                                                         >  2:2029807/1‑150 (MQ=255)
                                            ccTGGCTCAACGCCAAAGCCGGTCTGGGAGCGGATAAACTGCGCGCGGAACAGTTCACGCTCACGCGCGCCTTCCGCTGAGTTATCGGTTGCCGAGAAGAACCAGATGCCGAGGAATGCCACGGTGATAGAGAACAGCGCCGGGTATTCa                                                                                                         <  2:922462/150‑1 (MQ=255)
                                               tGCTCAACGCCAAAGCCGGTCTGGGAGCGGATAAACTGCGCGCGGAACAGTTCACGCTCACGCGCTCCTTCCGCTGAGTTATCGGTTGCCGAGAAGAACCAGATGCCGAGGAATGCCACGGTGATAGAGAACAGCGCCGGGTATTCATAc                                                                                                      >  1:947320/1‑150 (MQ=255)
                                               tGCTCAACGCCAAAGCCGGTCTGGGAGCGGATAAACTGCGCGCGGAACAGTTCACGCTCACGCGCGCCTTCCGCTGAGTTATCGGTTGCCGAGAAGAACCAGATGCCGAGGAATGCCACGGTGATAGAGAACAGCGCCGGGTATTCATAc                                                                                                      <  1:4498307/150‑1 (MQ=255)
                                               tGCTCAACGCCAAAGCCGGTCTGGGAGCGGATAAACTGCGCGCGGAACAGTTCACGCTCACGCGCGCCTTCCGCTGAGTTATCGGTTGCCGAGAAGAACCAGATGCCGAGGAATGCCACGGTGATAGAGAACAGCGCCGGGTATTCATAc                                                                                                      <  2:4286871/150‑1 (MQ=255)
                                                   cAACGCCAAAGCCGGTCTGGGAGCGGATAAACTGCGCGCGGAACAGTTCACGCTCACGCGCGCCTTCCGCTGAGTTATCGGTTGCCGAGAAGAACCAGATGCCGAGGAATGCCACGGTGATAGAGAACAGCGCCGGGTATTCATACGGGa                                                                                                  >  2:1516778/1‑150 (MQ=255)
                                                    aaCGCCAAAGCCGGTCTGGGAGCGGATAAACTGCGCGCGGAACAGTTCACGCTCACGCGCGCCTTCCGCTGAGTTATCGGTTGCCGAGAAGAACCAGATGCCGAGGAATGCCACGGTGATAGAGACCAGCGCCGGGTATTCATACGGGaa                                                                                                 >  2:3726126/1‑150 (MQ=255)
                                                      cGCCAAAGCCGGTCTGGGAGCGGATAAACTGCGCGCGGAACAGTTCACGCTCACGCGCGCCTTCCGCTGAGTTATCGGTTGCCGAGAAGAACCAGATGCCGAGGAATGCCACGGTGATAGAGAACAGCGCCGGGTATTCATACGGGAAGa                                                                                               <  2:1087939/150‑1 (MQ=255)
                                                      cGCCAAAGCCGGTCTGGGAGCGGATAAACTGCGCGCGGAACAGTTCACGCTCACGCGCGCCTTCCGCTGAGTTATCGGTTGCCGAGAAGAACCAGATGCCGAGGAATGCCACGGTGATAGAGAACAGCGCCGGGTATTCATACGGGAAGa                                                                                               >  1:1598689/1‑150 (MQ=255)
                                                         cAAAGCCGGTCTGGGAGCGGATAAACTGCGCGCGGAACAGTTCACGCTCACGCGCGCCTTCCGCTGAGTTATCGGTTGCCGAGAAGAACCAGATGCCGAGGAATGCCACGGTGATAGAGAACAGCGCCGGGTATTCATACGGGAAGATgg                                                                                            >  1:4323349/1‑150 (MQ=255)
                                                         cAAAGCCGGTCTGGGAGCGGATAAACTGCGCGCGGAACAGTTCACGCACACGCGCGCCTTCCGCTGAGTTATCGGTTGCCGAGAAGAACCAGATGCCGAGGAATGCCACGGTGATAGAGAACAGCGCCGGGTATTCATACGGGAAGATgg                                                                                            <  1:1559169/150‑1 (MQ=255)
                                                          aaaGCCGGTCTGGGAGCGGATAAACTGCGCGCGGAACAGTTCACGCTCACGCGCGCCTTCCGCTGAGTTATCGGTTGCCGAGAAGAACCTGATGCCGAGGAATGCCACGGTGATAGAGACCAGCGCCGGGTATTCATACGGGAAGATGGc                                                                                           <  1:3876797/150‑1 (MQ=255)
                                                           aaGCCGGTCTGGGAGCGGATAAACTGCGCGCGGAACAGTTCACGCTCACGCGCGCCTTCCGCTGAGTTATCGGTTGCCGAGAAGAACCAGATGCCGAGGAATGCCACGGTGATAGAGAACAGCGCCGGGTATTCATACGGGAAGATGGCt                                                                                          <  2:1634853/150‑1 (MQ=255)
                                                                ggTCTGGGAGCGGATAAACTGCGCGCGGAACAGTTCACGCTCACGCGCGCCTTCCGCTGAGTTATCGGTTGCCGAGAAGAACCAGATGCCGAGGAATGCCACGGTGATAGAGAACAGCGCCGGGTATTCATACGGGAAGATGGCTTTTTc                                                                                     <  1:644204/150‑1 (MQ=255)
                                                                ggTCTGGGAGCGGATAAACTGCGCGCGGAACAGTTCACGCTCACGCGCGCCTTCCGCTGAGTTATCGGTTGCCGAGAAGAACCAGATGCCGAGGAATGCCACGGTGATAGAGAACAGCGCCGGGTATTCATACGGGAAGATGGCTTTTTc                                                                                     <  2:706151/150‑1 (MQ=255)
                                                                   cTGGGAGCGGATAAACTGCGCGCGGAACAGTTCACGCTCACGCGCGCCTTCCGCTGAGTTATCGGTTGCCGAGAAGAACCTGATGCCGAGGAATGCCACGGTGATAGAGAACAGCGCCGGGTATTCATACGGGAAGATGGCTTTTTCGTg                                                                                  <  1:3776797/150‑1 (MQ=255)
                                                                    tGGGAGCGGATAAACTGCGCGCGGAACAGTTCACGCTCACGCGCGCCTTCCGCTGAGTTATCGGTTGCCGAGAAGAACCAGATGCCGAGGAATGCCACGGTGATAGAGAACAGCGCCGGGTATTCATACGGGAAGATGGCTTTTTCGTGa                                                                                 >  1:4339499/1‑150 (MQ=255)
                                                                     gggAGCGGATAAACTGCGCGCGGAACAGTTCACGCTCACGCGCGCCTTCCGCTGAGTTATCGGTTGCCGAGAAGAACCAGATGCCGAGGAATGCCACGGTGATAGAGAACAGCGCCGGGTATTCATACGGGAAGATGGCTTTTTCGTGAc                                                                                <  1:754191/150‑1 (MQ=255)
                                                                      ggAGCGGATAAACTGCGCGCGGAACAGTTCACGCTCACGCGCGCCTTCCGCTGAGTTATCGGTTGCCGAGAAGAACCAGATGCCGAGGAATGCCACGGTGATAGAGAACAGCGCCGGGTATTCATACGGGAAGATGGCTTTTTCGTGAcc                                                                               <  1:2450440/150‑1 (MQ=255)
                                                                         gCGGATAAACTGCGCGCGGAACAGTTCACGCTCACGCGCGCCTTCCGCTGAGTTATCGGTTGCCGAGAAGAACCAGATGCCGAGGAATGCCACGGTGATAGAGAACAGCGCCGGGTATTCATACGGGAAGATGGCTTTTTCGTGACCAAg                                                                            <  2:3253119/150‑1 (MQ=255)
                                                                          cGGATAAACTGCGCGCGGAACAGTTCACGCTCACGCGCGCCTTCCGCTGAGTTATCGGTTGCCGAGAAGAACCAGATGCCGAGGAATGCCACGGTGATAGAGAACAGCGCCGGGTATTCATACGGGAAGATGGCTTTTTCGTGACCAAgg                                                                           >  2:3232263/1‑150 (MQ=255)
                                                                           ggATAAACTGCGCGCGGAACAGTTCACGCTCACGCGCGCCTTCCGCTGAGTTATCGGTTGCCGAGAAGAACCAGATGCCGAGGAATGCCACGGTGATAGAGAACAGCGCCGGGTATTCATACGGGAAGATGGCTTTTTCGTGACCAAGGa                                                                          >  2:223023/1‑150 (MQ=255)
                                                                            gATAAACTGCGCGCGGAACAGTTCACGCTCACGCGCGCCTTCCGCTGAGTTATCGGTTGCCGAGAAGAACCAGATGCCGAGGAATGCCACGGTGATAGAGAACAGCGCCGGGTATTCATACGGGAAGATGGCTGTTTCGTGACCAAGGAt                                                                         >  2:4103904/1‑150 (MQ=255)
                                                                              tAAACTGCGCGCGGAACAGTTCACGCTCACGCGCGCCTTCCGCTGAGTTATCGGTTGCCGATAAGAACCAGATGCCGAGGAATGCCACGGTGATAGAGAACAGCGCCGGGTATTCATACGGGAAGATGGCTTTTTCGTGACCAAGGATCt                                                                       <  1:2289610/150‑1 (MQ=255)
                                                                              tAAACTGCGCGCGGAACAGTTCACGCTCACGCGCGCCTTCCGCTGAGTTATCGGTTGCCGAGAAGAACCAGATGCCGAGGAATGCCACGGTGATAGAGACCAGCGCCGGGTATTCATACGGGAAGATGGCTTTTTCGTGACCAAGGATCt                                                                       >  1:861127/1‑150 (MQ=255)
                                                                               aaaCTGCGCGCGGAACAGGTCACGCTCACGCGCGCCTTCCGCTGAGTTATCGGTTGCCGAGAAGAACCAGATGCCGAGGAATGCCACGGTGATAGAGAACAGCGCCGGGTATTCATACGGGAAGATGGCTTTTTCGTGACCAAGGATCTg                                                                      <  2:1433380/150‑1 (MQ=255)
                                                                                aaCTGCGCGCGGAACCGTTCACGCTCACGCGCGCCTTCCGCTGAGTTTTCGGTTGCCGAGAAGAACCAGATGCCTAGGAATGCCACGGTGATCGATAACAGCGCCGGGTATTCATACGGGAAGATGGCTTTTTCGTGACCAAGGATCTGt                                                                     <  1:4175046/150‑1 (MQ=255)
                                                                                 acggcgcgcGGAACAGTTCACGCTCACGCGCGCCTTCCGCTGAGTTATCGGTTGCCGAGAAGAACCAGATGCCGAGGAATGCCACGGTGATAGAGAACAGCGCCGGGTATTCATACGGGAAGATGGCTTTTTCGTGACCAAGGATCTGTa                                                                    <  2:3542025/147‑1 (MQ=255)
                                                                                 aCTGCGCGCGGAACAGTTCACGCTCACGCGCGCCTTCCGCTGAGTTATCGGTTGCCGAGAAGAACCAGATGCCGAGGAATGCCACGGTGATAGAGAACAGCGCCGGGTATTCATACGGGAAGATGGCTTTTTCGTGACCAAGGATCTGTa                                                                    <  2:3541768/150‑1 (MQ=255)
                                                                                   tGCGCGCGGAACAGTTCACGCTCACGCGCGCCTTCCGCTGAGTTATCGGTTGCCGAGAAGAACCTGATGCCGAGGAATGCCACGGTGATAGAGAACAGCGCCGGGTATTCATACGGGAAGATGGCTTTTTCGTGACCAAGGATCTGTAcc                                                                  >  1:525951/1‑150 (MQ=255)
                                                                                       cgcgGAACAGTTCACGCTCACGCGCGCCTTCCGCTGAGTTATCGGTTGCCGAGAAGAACCAGATGCCGAGGAATGCCACGGTGATAGAGAACAGCGCCGGGTATTCATACGGGAAGATGGCTTTTTCGTGACCAAGGATCTGTACCCaaa                                                              >  2:3503413/1‑150 (MQ=255)
                                                                                         cgGAACAGTTCACGCTCACGCGCGCCTTCCGCTGAGTTATCGGTTGCCGAGAAGAACCAGATGCCGAGGAATGCCACGGTGATAGAGAACAGCGCCGGGTATTCATACGGGAAGATGGCTTTTTCGTGACCAAGGATCTGTACCCAAAtc                                                            <  2:1138398/150‑1 (MQ=255)
                                                                                         cgGAACAGTTCACGCTCACGCGCGCCTTCCGCTGAGTTATCGGTTGCCGAGAAGAACCAGATGCCGAGGAATGCCACGGTGATAGAGAACAGCGCCGGGTATTCATACGGGAAGATGGCTTTTTCGTGACCAAGGATCTGTACCCAAAtc                                                            <  2:4364964/150‑1 (MQ=255)
                                                                                          ggAACAGTTCACGCTCACGCGCGCCTTCCGCTGAGTTATCGGTTGCCGAGAAGAACCAGATGCCGAGGAATGCCACGGTGATAGAGAACAGCGCCGGGTATTCATACGGGAAGATGGCTTTTTCGTGACCAAGGATCTGTACCCAAAtcg                                                           >  1:145322/1‑150 (MQ=255)
                                                                                          ggAACAGTTCACGCTCACGCGCGCCTTCCGCTGAGTTATCGGTTGCCGAGAAGAACCAGATGCCGAGGAATGCCACGGTGATAGAGAACAGCGCCGGGTATTCATACGGGAAGATGGCTTTTTCGTGACCAAGGATCTGTACCCAAAtcg                                                           >  1:3658437/1‑150 (MQ=255)
                                                                                          ggAACAGTTCACGCTCACGCGCGCCTTCCGCTGAGTTATCGGTTGCCGAGAAGAACCAGATGCCGAGGAATGCCACGGTGATAGAGAACAGCGCCGGGTATTCATACGGGAAGATGGCTTTTTCGTGACCAAGGATCTGTACCCAAAtcg                                                           >  1:3226378/1‑150 (MQ=255)
                                                                                              cAGTTCACGCTCACGCGCGCCTTCCGCTGAGTTATCGGTTGCCGAGAAGAACCAGATGCCGAGGAATGCCACGGTGATAGAGAACAGCGCCGGGTATTCATACGGGAAGATGGCTTTTTCGTGACCAAGGATCTGTACCCAAATCGTCgg                                                       >  1:2149899/1‑150 (MQ=255)
                                                                                              cAGTTCACGCTCACGCGCGCCTTCCGCTGAGTTATCGGTTGCCGAGAAGAACCAGATGCCGAGGAATGCCACGGTGATAGAGAACAGCGCCGGGTATTCATACGGGAAGATGGCTTTTTCGTGACCAAGGATCTGTACCCAAATCGTCgg                                                       <  2:2006188/150‑1 (MQ=255)
                                                                                              cAGTTCACGCTCACGCGCGCCTTCCGCTGAGTTATCGGTTGCCGAGAAGAACCAGATGCCGAGGAATGCCACGGTGATAGAGAACAGCGCCGGGTATTCATACGGGAAGATGGCTTTTTCGTGACCAAGGATCTGTACCCAAATCGTCgg                                                       <  2:1099313/150‑1 (MQ=255)
                                                                                               aGTTCACGCTCACGCGCGCCTTCCGCTGAGTTATCGGTTGCCGAGAAGAACCTGATGCCGAGGAATGCCACGGTGATAGAGAACAGCGCCGGGTATTCATACGGGAAGATGGCTTTTTCGTGACCAAGGATCTGTACCCAAATCGTCggg                                                      >  2:1631144/1‑150 (MQ=255)
                                                                                                  tcacgctcacgcGCGCCTTCCGCTGAGTTATCGGTTGCCGAGAAGAACCAGATGCCGAGGAATGCCACGGTGATAGAGAACAGCGCCGGGTATTCATACGGGAAGATGGCTTTTTCGTGACCAAGGATCTGTACCCAAATCGTCGGGCCg                                                   >  1:2710050/1‑150 (MQ=255)
                                                                                                   cacgctcacgcGCGCCTTCCGCTGAGTTATCGGTTGCCGAGAAGAACCAGATGCCGAGGAATGCCACGGTGATAGAGAACAGCGCCGGGTATTCATACGGGAAGATGGCTTTTTCGTGACCAAGGATCTGTACCCAAATCGTCGGGCCGa                                                  >  2:778927/1‑150 (MQ=255)
                                                                                                   cacgctcacgcGCGCCTTCCGCTGAGTTATCGGTTGCCGAGAAGAACCAGATGCCGAGGAATGCCACGGTGATAGAGAACAGCGCCGGGTATTCATACGGGAAGATGGCTTTTTCGTGACCAAGGATCTGTACCCAAATCGTCGGGCCGa                                                  >  2:1904216/1‑150 (MQ=255)
                                                                                                     cgctcacgcGCGCCTTCCGCTGAGTTATCGGTTGCCGAGAAGAACCAGATGCCGAGGAATGCCACGGTGATAGAGAACAGCGCCGGGTATTCATACGGGAAGATGGCTTTTTCGTGACCAAGGATCTGTACCCAAATCGTCGGGCCGAgg                                                >  1:70980/1‑150 (MQ=255)
                                                                                                       ctcacgcGCGCCTTCCGCTGAGTTATCGGTTGCCGAGAAGAACCAGATGCCGAGGAATGCCACGGTGATAGAGAACAGCGCCGGGTATTCATACGGGAAGATGGCTTTTTCGTGACCAAGGATCTGTACCCAAATCGTCGGGCCGAGGat                                              >  2:3869472/1‑150 (MQ=255)
                                                                                                        tcacgcGCGCCTTCCGCTGAGTTATCGGTTGCCGAGAAGAACCTGATGCCGAGGAATGCCACGGTGATAGAGAACAGCGCCGGGTATTCATACGGGAAGATGGCTTTTTCGTGACCAAGGATCTGTACCCAAATCGTCGGGCCGAGGatc                                             >  2:4064806/1‑150 (MQ=255)
                                                                                                        tcacgcGCGCCTTCCGCTGAGTTATCGGTTGCCGAGAAGAACCAGATGCCGAGGAATGCCACGGTGATAGAGAACAGCGCCGGGTATTCATACGGGAAGATGGCTTTTTCGTGACCAAGGATCTGTACCCAAATCGTCGGGCCGAGGatc                                             <  1:1752800/150‑1 (MQ=255)
                                                                                                         cacgcGCGCCTTCCGCTGAGTTATCGGTTGCCGAGAAGAACCAGATGCCGAGGAATGCCACGGTGATAGAGAACAGCGCCGGGTATTCATACGGGAAGATGGCTTTTTCGTGACCAAGGATCTGTACCCAAATCGTCGGGCCGAGGatca                                            <  1:988256/150‑1 (MQ=255)
                                                                                                          acgcGCGCCTTCCGCTGAGTTATCGGTTGCCGAGAAGAACCAGATGCCGAGGAATGCCACGGTGATAGAGAACAGCGCCGGGTATTCATACGGGAAGATGGCTTTTTCGTGACCAAGGATCTGTACCCAAATCGTCGGGCCGAGGatcat                                           <  2:2455205/150‑1 (MQ=255)
                                                                                                          acgcGCGCCTTCCGCTGAGTTATCGGTTGCCGAGAAGAACCAGATGCCGAGGAATGCCACGGTGATAGAGAACAGCGCCGGGTATTCATACGGGAAGATGGCTTTTTCGTGACCAAGGATCTGTACCCAAATCGTCGGGCCGAGGatcat                                           <  2:2735325/150‑1 (MQ=255)
                                                                                                             cgcgcCTTCCGCTGAGTTATCGGTTGCCGAGAAGAACCAGATGCCGAGGAATGCCACGGTGATAGAGAACAGCGCCGGGTATTCATACGGGAAGATGGCTTTTTCGTGACCAAGGATCTGTACCCAAATCGTCGGGCCGAGGATCATCAg                                        >  2:3152740/1‑150 (MQ=255)
                                                                                                               cgcCTTCCGCTGAGTTATCGGTTGCCGAGAAGAACCAGATGCCGAGGAATGCCACGGTGATAGAGAACAGCGCCGGGTATTCATACGGGAAGATGGCTTTTTCGTGACCAAGGATCTGTACCCAAATCGTCGGGCCGAGGATCATCAGta                                      <  1:1139738/150‑1 (MQ=255)
                                                                                                                   ttCCGCTGAGTTATCGGTTGCAGAGAAGAACCAGATGCCGAGGAATGCCACGGTGATAGAGAACAGCGCCGCGTATTCATACGGGAAGATGGCTTTTTCGTGACCAAGGATCTGTACCCAAATCGTCGGGCCGAGGATCATCAGtactac                                  >  1:2957161/1‑150 (MQ=255)
                                                                                                                    tCCGCTGAGTTATCGGTTGCCGAGAAGAACCAGATGCCGAGGAATGCCACGGTGATAGAGAACAGCGCCGGGTATTCATACGGGAAGATGGCTTTTTCGTGACCAAGGATCTGTACCCAAATCGTCGGGCCGAGGATCATCAGTactact                                 <  1:3719348/150‑1 (MQ=255)
                                                                                                                    tCCGCTGAGTTATCGGTTGCCGAGAAGAACCAGATGCCGAGGAATGCCACGGTGATAGAGAACAGCGCCGGGTATTCATACGGGAAGATGGCTTTTTCGTGACCAAGGATCTGTACCCAAATCGTCGGGCCGAGGATCATCAGTactact                                 <  2:2651996/150‑1 (MQ=255)
                                                                                                                      cGCTGAGTTATCGGTTGCCGAGAAGAACCAGATGCCGAGGAATGCCACGGTGATAGAGAACAGCGCCGGGTATTCATACGGGAAGATGGCTTTTTCGTGACCAAGGATCTGTACCCAAATCGTCGGGCCGAGGATCATCAGTACTACTGc                               <  2:3382923/150‑1 (MQ=255)
                                                                                                                       gCTGAGTTATCGGTTGCCGAGAAGAACCAGATGCCGAGGAATGCCACGGTGATAGAGAACAGCGCCGGGTATTCATACGGGAAGATGGCTTTTTCGTGACCAAGGATCTGTACCCAAATCGTCGGGCCGAGGATCATCAGTACTACTGCg                              >  2:2380279/1‑150 (MQ=255)
                                                                                                                        cTGAGTTATCGGTTGCCGAGAAGAACCAGATGCCGAGGAATGCCACGGTGATAGAGAACAGCGCCGGGTATTCATACGGGAAGATGGCTTTTTCGTGACCAAGGATCTGTACCCAAATCGTCGGGCCGAGGATCATCAGTACTACTGCgg                             >  2:1292959/1‑150 (MQ=255)
                                                                                                                          gAGTTATCGGTTGCCGAGAAGAACCAGATGCCGAGGAATGCCACGGTGATAGAGAACAGCGCCGGGTATTCATACGGGAAGATGGCTTTTTCGTGACCAAGGATCTGTACCCAAATCGTCGGGCCGAGGATCATCAGTACTACTGCGGTa                           >  1:4394144/1‑150 (MQ=255)
                                                                                                                          gAGTTATCGGTTGCCGAGAAGAACCAGATGCCGAGGAATGCCACGGTGATAGAGAACAGCGCCGGGTATTCATACGGGAAGATGGCTTTTTCGTGACCAAGGATCTGTACCCAAATCGTCGGGCCGAGGATCATCAGTACTACTGCGGTa                           >  1:2942239/1‑150 (MQ=255)
                                                                                                                            gTTATCGGTTGCCGAGAAGAACCAGATGCCGAGGAATGCCACGGTGATAGAGAACAGCGCCGGGTATTCATACGGGAAGATGGCTTTTTCGTGACCAAGGATCTGTACCCAAATCGTCGGGCCGAGGATCATCAGTACTACTGCGGTAAt                         <  1:254968/150‑1 (MQ=255)
                                                                                                                                  ggTTGCCGAGAAGAACCAGATGCCGAGGAATGCCACGGTGATAGAGAACAGCGCCGGGTATTCATACGGGAAGATGGCTTTTTCGTGACCAAGGATCTGTACCCAAATCGTCGGGCCGAGGATCATCAGTACTACTGCGGTAATCAGccc                   >  2:307533/1‑150 (MQ=255)
                                                                                                                                    ttGCCGAGAAGAACCTGATGCCGAGGAATGCCACGGTGATAGAGAACAGCGCCGGGTATTCATACGGGAAGATGGCTTTTTCGTGACCAAGGATCTGTACCCAAATCGTCGGGCCGAGGATCATCAGTACTACTGCGGTAATCAGCCcca                 <  1:792075/150‑1 (MQ=255)
                                                                                                                                        cGAGAAGAACCAGATGCCGAGGAATGCCACGGTGATAGAGAACAGCGCCGGGTATTCATACGGGAAGATGGCTTTTTCGTGACCAAGGATCTGTACCCAAATCGTCGGGCCGAGGATCATCAGTACTACTGCGGTAATCAGCCccagcca             >  2:3015991/1‑150 (MQ=255)
                                                                                                                                         gagaAGAACCAGATGCCGAGGAATGCCACGGTGATAGAGAACAGCGCCGGGTATTCATACGGGAAGATGGCTTTTTCGTGACCAAGGATCTGTACCCAAATCGTCGGGCCGAGGATCATCAGTACTACTGCGGTAATCAGCCccagccag            >  1:4020289/1‑150 (MQ=255)
                                                                                                                                         gagaAGAACCAGATGCCGAGGAATGCCACGGTGATAGAGAACAGCGCCGGGTATTCATACGGGAAGATGGCTTTTTCGTGACCAAGGATCTGTACCCAAATCGTCGGGCCGAGGATCATCAGTACTACTGCGGTAATCAGCCccagccag            <  1:750520/150‑1 (MQ=255)
                                                                                                                                         gagaAGAACCAGATGCCGAGGAATGCCACGGTGATAGAGAACAGCGCCGGGTATTCATACGGGAAGATGGCTTTTTCGTGACCAAGGATCTGTACCCAAATCGTCGGGCCGAGGATCATCAGTACTACTGCGGTAATCAGCCccagccag            >  2:1490438/1‑150 (MQ=255)
                                                                                                                                          agaagaACCAGATGCCGAGGAATGCCACGGTGATAGAGAACAGCGCCGGGTATTCATACGGGAAGATGGCTTTTTCGTGACCAAGGATCTGTACCCAAATCGTCGGGCCGAGGATCATCAGTACTACTGCGGTAATCAGCCCcagccagc           >  2:3640884/1‑150 (MQ=255)
                                                                                                                                                aCCAGATGCCGAGGAATGCCCCGGGGATAGAGAACAGCGCCGGGTATTCATACGGGAAGATGGCTTTTTCGTGACCAAGGATCTGTACCCAAATCGTCGGGCCGAGGATCATCAGTACTACTGCGGTAATCAGCCCCAGCCAGCCACCca     <  1:2357306/150‑1 (MQ=255)
                                                                                                                                                aCCAGATGCCGAGGAATGCCACGGTGATAGAGAACAGCGCCGGGTATTCATACGGGAAGATGGCTTTTTCGTGACCAAGGATCTGTACCCAAATCGTCGGGCCGAGGATCATCAGTACTACTGCGGTAATCAGCCCCAGCCAGCCACCca     >  2:2857784/1‑150 (MQ=255)
                                                                                                                                                 ccAGATGCCGAGGAATGCCACGGTGATAGAGAACAGCGCCGGGTATTCATACGGGAAGATGGCTTTTTCGTGACCAAGGATCTGTACCCAAATCGTCGGGCCGAGGATCATCAGTACTACTGCGGTAATCAGCCCCAGCCAGCCACCcat    >  1:4387143/1‑150 (MQ=255)
                                                                                                                                                   aGATGCCGAGGAATGCCACGGTGATAGAGAACAGCGCCGGGTATTCATACGGGAAGATGGCTTTTTCGTGACCAAGGATCTGTACCCAAATCGTTGGGCCGAGGATCATCAGTACTACTGCGGTAATCAGCCCCAGCCAGCCACCcatca  <  1:1301656/150‑1 (MQ=255)
                                                                                                                                                   aGATGCCGAGGAATGCCACGGTGATAGAGAACAGCGCCGGGTATTCATACGGGAAGATGGCTTTTTCGTGACCAAGGATCTGTACCCAAATCGTCGGGCCGAGGATCATCAGTACTACTGCGGTAATCAGCCCCAGCCAGCCACCcatca  >  2:493610/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                                     
CCCCGGTCAGACGACCGGGGAAGGGGAGAGATTAATGCGCGCGGCCTTGCTCAACGCCAAAGCCGGTCTGGGAGCGGATAAACTGCGCGCGGAACAGTTCACGCTCACGCGCGCCTTCCGCTGAGTTATCGGTTGCCGAGAAGAACCAGATGCCGAGGAATGCCACGGTGATAGAGAACAGCGCCGGGTATTCATACGGGAAGATGGCTTTTTCGTGACCAAGGATCTGTACCCAAATCGTCGGGCCGAGGATCATCAGTACTACTGCGGTAATCAGCCCCAGCCAGCCACCCATCA  >  CP009273/4273150‑4273446

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑