Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 4,275,142 C→A 57.9% intergenic (‑1/+199) yjcH ← / ← acs DUF485 family inner membrane protein/acetyl‑CoA synthetase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092734,275,1420CA57.9% 72.7 / inf 274intergenic (‑1/+199)yjcH/acsDUF485 family inner membrane protein/acetyl‑CoA synthetase
Reads supporting (aligned to +/- strand):  ref base C (73/42);  new base A (79/80);  total (152/122)
Fisher's exact test for biased strand distribution p-value = 2.68e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.47e-01

Only 100 of 291 total aligned reads displayed.

AGCCGGGCGCGAAGGCGATCAGTAAAATAAAGCCGATATAAACTGCCAGCATAATAATCGACAGGATGGTGGCAAACCGTTGCCGTTTTTCGACTAACTCCCTGAAATGCGCATTGTCTTCTATCCGCTGATAAATAGTGCCATTCATCACAGATTCTCCAGAGGTAATGTAGGGATTGTTTTAATTCCCGCTCCCTTATGGGAGAAGGTTAACGCTCGGGTAACCCTTGCCGAATGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCG  >  CP009273/4274994‑4275291
                                                                                                                                                    |                                                                                                                                                     
     ggCGCGAAGGCGATCAGTAAAATAAAGCCGATATAAACTGCCAGCATAATAATCGACAGGATGGTGGCAAACCGTTGCCGTTTTTCGACTAACTCCCTGAAATGCGCATTGTCTTCTATCCGCTGATAAATAGTGCCATTCATAACAGAt                                                                                                                                                 <  1:1700367/150‑1 (MQ=255)
       cgcgAAGGCGATCAGTAAAATAAAGCCGATATAAACTGCCAGCATAATAATCGACAGGATGGTGGCAAACCGTTGCCGTTTTTCGACTAACTCCCTGACATGCGCATTGTCTTCTATCCGCTGATAAATAGTGCCATTCATAACAGATtc                                                                                                                                               >  2:3574935/1‑150 (MQ=255)
          gAAGGCGATAAGAAAAATAAAGCCGATATAAACTGCCAGCATAATAATCGACAGGATGGTGGCAAACCGTTGCCGTTTTTCGACTAACTCCCTGAAATGCGCATGGTCTTCTATCCGCTGATAAATAGTGCCATTCATCACAGATTCTcc                                                                                                                                            <  1:3997602/150‑1 (MQ=255)
           aaGGCGATCAGTAAAATAAAGCCGATATAAACTGCCAGCATAATACTCGACAGGATGGTGGCAAACCGTTGCCGTTTTTCGACTAACTCCCTGAAATGCGCATTGTCTTCTATCCGCTGATAAATAGTGCCATTCATAACAGATTCTCCa                                                                                                                                           <  1:3874549/150‑1 (MQ=255)
           aaGGCGATCAGTAAAATAAAGCCGATATAAACTGCCAGCATAATAATCGACAGGATGGTGGCAAACCGTTGCCGTTTTTCGACTAACTCCCTGAAATGCGCATTGTCTTCTATCCGCTGATAAATAGTGCCATTCATCACAGATTCTCCa                                                                                                                                           >  2:1090031/1‑150 (MQ=255)
              gCGATCAGTAAAATAAAGCCGATATAAACTGCCAGCATAATAATCGACAGGATGGTGGCAAACCGTTGCCGTTTTTCGACTAACTCCCTGAAATGCGCATTGTCTTCTATCCGCTTATAAATAGTGCCATTCATCACAGATTCTCCagag                                                                                                                                        <  1:1628366/150‑1 (MQ=255)
                gATCAGTAAAATAAAGCCGATATAAACTGCCAGCATAATAATCGACAGGATGGTGGCAAACCGTTGCCGTTTTTCGACTAACTCCCTGAAATGCGCATTGTCTTCTATCCGCTTATAAATAGTGCCATTCATCACAGATTCTCCAGAGGt                                                                                                                                      <  1:2853903/150‑1 (MQ=255)
                 aTCAGTAAAATAAAGCCGATATAAACTGCCAGCATAATAATCGACAGGATGGTGGCAAACCGTTGCCGTTTTTCGACTAACTCCCTGAAATGCGCATTGTCTTCTATCCGCTGATAAATAGTGCCATTCATAACAGATTCTCCAGAGGTa                                                                                                                                     >  2:256377/1‑150 (MQ=255)
                 aTCAGTAAAATAAAGCCGATATAAACTGCCAGCATAATAATCGACAGGATGGTGGCAAACCGTTGCCGTTTTTCGACTAACTCCCTGAAATGCGCATTGTCTTCTATCCGCTGATAAATAGTGCCATTCATAACAGATTCTCCAGAGGTa                                                                                                                                     >  2:255523/1‑150 (MQ=255)
                 aTCAGTAAAATAAAGCCGATATAAACTGCCAGCATAATAATCGACAGGATGGTGGCAAACCGTTGCCGTTTTTCGACTAACTCCCTGAAATGCGCATTGTCTTCTATCCGCTGATAAATAGTGCCATTCATAACAGATTCTCCAGAGGTa                                                                                                                                     <  2:4300931/150‑1 (MQ=255)
                  tCAGTAAAATAAAGCCGATATAAACTGCCAGCATAATAATCGACAGGATGGTGGCAAACCGTTGCCGTTTTTCGACTAACTCCCTGAAATGCGCATTGTCTTCTATCCGCTGATAAATAGTGCCATTCATAACAGATTCTCCAGAGGtca                                                                                                                                    >  2:1221314/1‑148 (MQ=255)
                  tCAGTAAAATAAAGCCGATATAAACTGCCAGCATAATAATCGACAGGATGGTGGCAAACCGTTGCCGTTTTTCGACTAACTCCCTGAAATGCGCATTGTCTTCTATCCGCTGATAAATAGTGCCATTCATAACAGATTCTCCAGAGGTaa                                                                                                                                    >  2:3506817/1‑150 (MQ=255)
                       aaaaTAAAGCCGATATAAACTGCCAGCATAATAATCGACAGGATGGTGGCAAACCGTTGCCGTTTTTCGACTAACTCCCTGAAATGCGCATTGTCTTCTATCCGCTGATAAATAGTGCCATTCATCACAGATTCTCCAGAGGTAATGTAg                                                                                                                               >  1:2722526/1‑150 (MQ=255)
                       aaaaTAAAGCCGATATAAACTGCCAGCATAATAATCGACAGGATGGTGGCAAACCGTTGCCGTTTTTCGACTAACTCCCTGAAATGCGCATTGTCTTCTATCCGCTGATAAATAGTGCCATTCATAACAGATTCTCCAGAGGTAATGTAg                                                                                                                               <  1:2032813/150‑1 (MQ=255)
                          aTAAAGCCGATATAAACTGCCAGCATAATAATCGACAGGATGGTGGCAAACCGTTGCCGTTTTTCGACTAACTCCCTGAAATGCGCATTGTCTTCTATCCGCTGATAAATAGTGCCATTCATAACAGATTCTCCAGAGGTAATGTAGGGa                                                                                                                            >  2:1457241/1‑150 (MQ=255)
                            aaaGCCGATATAAACTGCCAGCATACTAATCGACAGGATGGTGGCAAACCGTTGCCGTTTTTCGACTAACTCCCTGAAATGCGCATTGTCTTCTATCAGCTGATAAATAGTGCCATTCATCACAGATTCTCCAGAGGTAATGTCGGGAtt                                                                                                                          >  1:3148082/1‑150 (MQ=255)
                             aaGCCGATATAAACTGCCAGCATAATAATCGACAGGATGGTGGCAAACCGTTGCCGTTTTTCGACTAACTCCCTGAAATGCGCATTGTCTTCTATCCGCTTATAAATAGTGCCATTCATCACAGATTCTCCAGAGGTAATGTAGGGATTg                                                                                                                         >  2:3748414/1‑150 (MQ=255)
                               gCCGATATAAACTGCCAGCATAATAATCGACAGGATGGTGGCAAACCGTTGCCGTTTTTCGACTAACTCCCTGAAATGCGCATTGTCTTCTATCCGCTTATAAATAGTGCCATTCATCACAGATTCTCCAGAGGTAATGTAGGGAGTGtt                                                                                                                       >  1:2315209/1‑150 (MQ=255)
                                 cGATATAACCGGCCAGCATAATACTCGACAGGATGGTGGCAAACCGTTGCCGTTTTTCGACTAACTCCCTGAAATGCGCATGGTCTTCTATCCGCTGATAAATAGTGCCATTCATAACAGATTCTCCAGAGGTAATGTAGGGATTGtttt                                                                                                                     <  1:4324990/150‑1 (MQ=255)
                                 cGATATAACCGGCCAGCATAATAATCGACAGGATGGTGGCAAACCGTTGCCGTTTTTCGACTAACTCCCTGAAATGCGCAGTGTCTTCTATCCGCTGATAAATAGTGCCATTCATAACAGATTCTCCAGAGGTAATGTAGGGATTGtttt                                                                                                                     <  1:127372/150‑1 (MQ=255)
                                 cGATATAAACTGCCAGCATAATAATCGACAGGATGGTGGCAAACCGTTGCCGTTTTTCGACTAACTCCCTGAAATGCGCATTGTCTTCTATCCGCTGATAAATAGTGCCATTCATAACAGATTCTCCAGAGGTAATGTAGGGATTGtttt                                                                                                                     <  2:2612776/150‑1 (MQ=255)
                                   atatAAACTGCCAGCATAATAATCGACAGGATGGTGGCAAACCGTTGCCGTTTTTCGACTAACTCCCTGACATGCGCATTGTCTTCTATCCGCTGATAAATAGTGCCATTCATCACAGATTCTCCAGAGGTAATGTAGGGATTGTTTTaa                                                                                                                   >  2:3929996/1‑150 (MQ=255)
                                    tataAACTGCCAGCATAATAATCGACAGGATGGTGGCAAACCGTTGCCGTTTTTCGACTAACTCCCTGAAATGCGCATTGTCTTCTATCCGCTTATAAATAGTGCCATTCATCACAGATTCTCCAGAGGTAATGTAGGGATTGTTTTAAt                                                                                                                  >  2:3162011/1‑150 (MQ=255)
                                      taAACTGCCAGCATAATAATCGACAGGATGGTGGCAAACCGTTGCCGTTTTTCGACTAACTCCCTGAAATGCGCATTGTCTTCTATCCGCTGATAAATAGTGCCATTCATAACAGATTCTCCAGAGGTAATGTAGGGATTGTTTTAATTc                                                                                                                >  2:3086307/1‑150 (MQ=255)
                                       aaaCTGCCAGCATAATAATCGACAGGATGGTGGCAAACCGTTGCCGTTTTTCGACTAACTCCCTGAAATGCGCATTGTCTTCTATCCGCTTATAAATAGTGCCATTCATCACAGATTCTCCAGAGGTAATGTAGGGATTGTTTTAATTcc                                                                                                               >  1:1040994/1‑150 (MQ=255)
                                       aaaCTGCCAGCATAATAATCGACAGGATGGTGGCAAACCGTTGCCGTTTTTCGACTAACTCCCTGAAATGCGCATTGTCTTCTATCCGCTGATAAATAGTGCCATTCATAACAGATTCTCCAGAGGTAATGTAGGGATTGTTTTAATTcc                                                                                                               >  2:641990/1‑150 (MQ=255)
                                        aaCTGCCAGCATAATAATCGACAGGATGGTGGCAAACCGTTGCCGTTTTTCGACTAACTCCCTGAAATGCGCATTGTCTTCTATCCGCTGATAAATAGTGCCATTCATAACAGATTCTCCAGAGGTAATGTAGGGATTGTTTTAATTccc                                                                                                              >  2:3709723/1‑150 (MQ=255)
                                        aaCTGACAGCATAATAATCGACAGGATGGTGGCAAACCGTTGCCGTTTTTCGACTAACTACCTGAAATGCGCATGGTCTTCTATCCGCTGATAAATAGTGCCATTCATAACAGATTCTCCAGATGTAATGTAGGGATTGTTTTAATTccc                                                                                                              <  1:2165946/150‑1 (MQ=255)
                                              cAGCATAATAATCGACAGGATGGTGGCAAACCGTTGCCGTTTTTCGACTAACTCCCTGAAATGCGCATTGTCTTCTATCCGCTTATAAATAGTGCCATTCATCACAGATTCTCCAGAGGTAATGTAGGGATTGTTTTAATTCCCGCTccc                                                                                                        >  2:1855159/1‑150 (MQ=255)
                                              cAGCATAATAATCGACAGGATGGTGGCAAACCGTTGCCGTTTTTCGACTAACTCCCTGAAATGCGCATTGTCTTCTATCCGCTTATAAATAGTGCCATTCATCACAGATTCTCCAGAGGTAATGTAGGGATTGTTTTAATTCCCGCTccc                                                                                                        >  2:981688/1‑150 (MQ=255)
                                               aGCATAATAATCGACAGGATGGTGGCAAACCGTTGCCGTTTTTCGACTAACTCCCTGAAATGCGCATTGTCTTCTATCCGCTGATAAATAGTGCCATTCATAACAGATTCTCCAGAGGTAATGTAGGGATTGTTTTAATTCCCGCTCCCt                                                                                                       <  1:2469612/150‑1 (MQ=255)
                                               aGCATAATAATCGACAGGATGGTGGCAAACCGTTGCCGTTTTTCGACTAACTCCCTGAAATGCGCATTGTCTTCTATCCGCTGATAAATAGTGCCATTCATAACAGATTCTCCAGAGGTAATGTAGGGATTGTTTTAATTCCCGCTCCCt                                                                                                       <  1:1802025/150‑1 (MQ=255)
                                               aGCATAATAATCGACAGGATGGTGGCAAACCGTTGCCGTTTTTCGACTAACTCCCTGAAATGCGCAGTGTCTTCTATCCGCTGATAAATAGTGCCATTCATAACAGATTCTCCAGAGGTAATGTAGGGATTGTTTTAATTCCCGCTCCCt                                                                                                       <  2:750799/150‑1 (MQ=255)
                                                gCATAATAATCGACAGGATGGTGGCAAACCGTTGCCGTTTTTCGACTAACTCCCTGAAATGCGCATTGTCTTCTATCCGCTGATAAATAGTGCCATTCATCTCAGATTCTCCAGAGGTAATGTAGGGATTGTTTTAATTCCCGCTCCCtt                                                                                                      >  2:3809125/1‑150 (MQ=255)
                                                gCATAATAATCGACAGGATGGTGGCAAACCGTTGCCGTTTTTCGACTAACTCCCTGAAATGCGCATTGTCTTCTATCCGATGATAAATAGTGCCATTCATCACAGATTCTCCAGAGGTAATGTAGGGATTGTTTTAATTCCCGCTCCCtt                                                                                                      <  1:1535430/150‑1 (MQ=255)
                                                 cATAATAATCGACAGGATGGTGGCAAACCGTTGCCGTTTTTCGACTAACTCCCTGAAATGCGCATTGTCTTCTATCCGCTGATAAATAGTGCCATTCATCACAGATTCTCCAGAGGTAATGTAGGGATTGTTTTAATTCCCGCTCCCTTa                                                                                                     >  2:1727932/1‑150 (MQ=255)
                                                 cATAATAATCGACAGGATGGTGGCAAACCGTTGCCGTTTTTCGACTAACTCCCTGAAATGCGCATTGTCTTCTATCCGCTGATAAATAGTGCCATTCATCACAGATTCTCCAGAGGTAATGTAGGGATTGTTTTAATTCCCGCTCCCTTa                                                                                                     >  2:4005341/1‑150 (MQ=255)
                                                  ataataATCGACAGGATGTTGGCAAACCGTTGCCGTTTTTCGACTAACTCCCTGAAATGCGCATTGTCTTCTATCCGCTGATAAATAGTGCCATTCATAACAGATTCTCCAGAGGTAATGTAGGGATTGTTTTAATTCCCGCTCCCTTAt                                                                                                    >  2:3349090/1‑150 (MQ=255)
                                                  ataataATCGACAGGATGGTGGCAAACCGTTGCCGTTTTTCGACTAACTCCCTGAAATGCGCATTGTCTTCTATCCGCTGATAAATAGTGCCATTCATAACAGATTCTCCAGAGGTAATGTAGGGATTGTTTTAATTCCCGCTCCCTTAt                                                                                                    <  1:3547051/150‑1 (MQ=255)
                                                    aataatCGACAGGATGGTGGCAAACCGTTGCCGTTTTTCGACTAACTCCCTGAAATGCGCATTGTCTTCTATCCGCTGATAAATAGTGCCATTCATCACAGATTCTCCAGAGGTAATGTAGGGATTGTTTTAATTCCCGCTCCCTTATgg                                                                                                  <  1:1932533/150‑1 (MQ=255)
                                                     ataatCGACAGGATGGTGGCAAACCGTTGCCGTTTTTCGACTAACTCCCTGAAATGCGCATTGTCTTCTATCCGCTGATAAATAGTGCCATTCATAACAGATTCTCCAGAGGTAATGTAGGGATTGTTTTAATTCCCGCTCCCTTATggg                                                                                                 <  1:1663691/150‑1 (MQ=255)
                                                     ataatCGACAGGATGGTGGCAAACCGTTGCCGTTTTTCGACTAACTCCCTGAAATGCGCATTGTCTTCTATCCGCTGATAAATAGTGCCATTCATAAAAGATTCTCCAGAGGTAATGTAGGGATTGTTTTAATTCCCGCTCCCTTATggg                                                                                                 >  1:732666/1‑150 (MQ=255)
                                                       aatCGACAGGATGGTGGCAAACCGTTGCCGTTTTTCGACTAACTCCCTGAAATGCGCATTGTCTTCTATCCGCTGATAAATAGTGCCATTCATAACAGATTCTCCAGAGGTAATGTAGGGATTGTTTTAATTCCCGCTCCCTTATGGgag                                                                                               <  1:1023677/150‑1 (MQ=255)
                                                          cGACAGGATGGTGGCAAACCGTTGCCGTTTTTCGACTAACTCCCTGAAATGCGCATTGTCTTCTATCCGCTTATAAATAGTGCCATTCATCACAGATTCTCCAGAGGTAATGTAGGGATTGTTTTAATTCCCGCTCCCTTATGGGAGAAg                                                                                            <  1:329945/150‑1 (MQ=255)
                                                             cAGGATGGTGGCAAACCGTTGCCGTTTTTCGACTAACTCCCTGAAATGCGCATTGTCTTCTATCCGCTGATAAATAGTGCCATTCATAACAGATTCTCCAGAGGTAATGTAGGGATTGTTTTAATTCCCGCTCCCTTATGGGAGAAGGtt                                                                                         <  2:2374229/150‑1 (MQ=255)
                                                              aGGATGGTGGCAAACCGTTGCCGTTTTTCGACTAACTCCCTGAAATGCGCATTGTCTTCTATCCGCTGATAAATAGTGCCATTCATAACAGATTCTCCAGAGGTAATGTAGGGATTGTTTTAATTCCCGCTCCCTTATGGGAGAAGGTTa                                                                                        >  2:3844787/1‑150 (MQ=255)
                                                              aGGATGGTGGCAAACCGTTGCCGTTTTTCGACTAACTCCCTGAAATGCGCATTGTCTTCTATCCGCTGATAAATAGTGCCATTCATAACAGATTCTCCAGAGGTAATGTAGGGATTGTTTTAATTCCCGCTCCCTTATGGGAGAAGGTTa                                                                                        <  2:2346410/150‑1 (MQ=255)
                                                              aGGATGGTGGCAAACCGTTGCCGTTTTTCGACTAACTCCCTGAAATGCGCATTGTCTTCTATCCGCTGATAAATAGTGCCATTCATAACAGATTCTCCAGAGGTAATGTAGGGATTGTTTTAATTCCCGCTCCCTTATGGGAGAAGGTTa                                                                                        >  2:2202177/1‑150 (MQ=255)
                                                                         aaaCCGTTGCCGTTTTTCGACTAACTCCCTGAAATGCGCATTGTCTTCTATCCGCTGATAAATAGTGCCATTCATAACAGATTCTCCAGAGGTAATGTAGGGATTGTTTTAATTCCCGCTCCCTTATGGGAGAAGGTTAACGCTCGGGTa                                                                             >  2:2458302/1‑150 (MQ=255)
                                                                          aaCCGTTGCCGTTTTTCGACTAACTCCCTGAAATGCGCATTGTCTTCTATCCGCTGATAAATAGTGCCATTCATAACAGATTATCCAGAGGTAATGTAGGGATTGTTTTAATTCCCGCTCCCTTATGGGAGAAGGTTAACGCTCGGGTaa                                                                            >  1:1434431/1‑150 (MQ=255)
                                                                           aCCGTTGCCGTTTTTCGACTAACTCCCTGAAATGCGCATTGTCTTCTATCCGCTGATAAATAGTGCCATTCATAACAGATTCTCCAGAGGTAATGTAGGGATTGTTTTAATTCCCGCTCCCTTATGGGAGAAGGTTAACGCTCGGGTAAc                                                                           <  2:3292514/150‑1 (MQ=255)
                                                                               ttGCCGTTTTTCGACTAACTCCCTGAAATGCGCATTGTCTTCTATCCGCTTATAAATAGTGCCATTCATCACAGATTCTCCAGAGGTAATGTAGGGATTGTTTTAATTCCCGCTCCCTTATGGGAGAAGGTTAACGCTCGGGTAACCCtt                                                                       >  2:4033176/1‑150 (MQ=255)
                                                                                 gCCGTTTTTCGACTAACTCCCTGAAATGCGCATTGTCTTCTATCCGCTTATAAATAGTGCCATTCATCACAGATTCTCCAGAGGTAATGTAGGGATTGTTTTAATTCCCGCTCCCTTATGGGAGAAGGTTAACGCTCGGGTAACCCTTGc                                                                     >  1:4117275/1‑150 (MQ=255)
                                                                                    gTTTTTCGACTAACTCCCTGAAATGCGCATTGTCTTCTATCCGCTGATAAATAGTGCCATTCATAACAGATTCTCCAGAGGTAATGTAGGGATTGTTTTAATTCCCGCTCCCTTATGGGAGAAGGTTAACGCTCGGGTAACCCTTGCCGa                                                                  >  1:4529798/1‑150 (MQ=255)
                                                                                          cGACTAACTCCCTGAAATGCGCATTGTCTTCTATCCGCTGATAAATAGTGCCATTCATCACAGATTCTCCAGAGGTAATGTAGGGATTGTTTTAATTCCCGCTCCCTTATGGGAGAAGGTTAACGCTCGGGTAACCCTTGCCGAATGTAg                                                            <  2:1051686/150‑1 (MQ=255)
                                                                                           gACTAACTCCCTGAAATGCGCATTGTCTTCTATCCGCTTATAAATAGTGCCATTCATCACAGATTCTCCAGAGGTAATGTAGGGATTGTTTTAATTCCCGCTCCCTTATGGGAGAAGGTTAACGCTCGGGTAACCCTTGCCGAATGTAgg                                                           >  2:3448914/1‑150 (MQ=255)
                                                                                           gACTAACTCCCTGAAATGCGCATTGTCTTCTATCCGCTTATAAATAGTGCCATTCATCACAGATTCTCCAGAGGTAATGTAGGGATTGTTTTAATTCCCGCTCCCTTATGGGAGAAGGTTAACGCTCGGGTAACCCTTGCCGAATGTAgg                                                           >  1:4420012/1‑150 (MQ=255)
                                                                                           gACTAACTCCCTGAAATGCGCATTGTCTTCTATCCGCTGATAAATAGTGCCATTCATAACAGATTCTCCAGAGGTAATGTAGGGATTGTTTTAATTCACGCTCCCTTATGGGAGAAGGTTAACGCTCGGGTCACCCGTGCCGAATGTAgg                                                           >  2:4392663/1‑150 (MQ=255)
                                                                                               aaCTCCCTGAAATGCGCATTGTCTTCTATCCGCTGATAAATAGTGCCATTCATAACAGATTCTCCAGAGGTAATGTAGGGATTGTTTTAATTCCCGCTCCCTTATGGGAGAAGGTTAACGCTCGGGTAACCCTTGCCGAATGTAGGCCgg                                                       >  1:2249702/1‑150 (MQ=255)
                                                                                                 cTCCCTGAAATGCGCATTGTCTTCTATCCGCTGATAAATAGTGCCATTCATAACAGATTCTCCAGAGGTAATGTAGGGATTGTTTTAATTCCCGCTCCCTTATGGGAGAAGGTTAACGCTCGGGTAACCCTTGCCGAATGTAGGCCGGAt                                                     <  1:1324485/150‑1 (MQ=255)
                                                                                                   cccTGAAATGCGCATTGTCTTCTATCCGCTGATAAATAGTGCCATTCATAACAGATTCTCCAGAGGTAATGTAGGGATTGTTTTAATTCCCGCTCCCTTATGGGAGAAGGTTAACGCTCGGGTAACCCTTGCCGAATGTAGGCCGGATaa                                                   >  1:2759918/1‑150 (MQ=255)
                                                                                                    ccTGAAATGCGCATTGTCTTCTATCCGCTGATAAATAGTGCCATTCATAACAGATTCTCCAGAGGTAATGTAGGGATTGTTTTAATTCCCGCTCCCTTATGGGAGAAGGTTAACGCTCGGGTAACCCTTGCCGAATGTAGGCCGGATAAg                                                  >  2:1014137/1‑150 (MQ=255)
                                                                                                    ccTGAAATGCGCATTGTCTTCTATCCGCTGATAAATAGTGCCATTCATAACAGATTCTCCAGAGGTAATGTAGGGATTGTTTTAATTCCCGCTCCCTTATGGGAGAAGGTTAACGCTCGGGTAACCCTTGCCGAATGTAGGCCGGATAAg                                                  >  2:3244595/1‑150 (MQ=255)
                                                                                                      tGAAATGCGCATTGTCTTCTATCCGCTGATAAATAGTGCCATTCATCACAGATTCTCCAGAGGTAATGTAGGGATTGTTTTAATTCCCGCTCCCTTATGGGAGAAGGTTAACGCTCGGGTAACCCTTGCCGAATGTAGGCCGGATAAGGc                                                <  2:1386188/150‑1 (MQ=255)
                                                                                                      tGAAATGCGCATTGTCTTCTATCCGCTGATAAATAGTGCCATTCATAACAGATTCTCCAGAGGTAATGTAGGGATTGTTTTAATTCCCGCTCCCTTATGGGAGAAGGTTAACGCTCGGGTAACCCTTGCCGAATGTAGGCCGGATAAGGc                                                >  1:87023/1‑150 (MQ=255)
                                                                                                      tGAAATGCGCATTGTCTTCTATCCGCTGATAAATAGTGCCATTCATAACAGATTCTCCAGAGGTAATGTAGGGATTGTTTTAATTCCCGCTCCCTTATGGGAGAAGGTTAACGCTCGGGTAACCCTTGCCGAATGTAGGCCGGATAAGGc                                                >  1:3732024/1‑150 (MQ=255)
                                                                                                       gAAATGCGCATTGTCTTCTATCCGCTGATAAATAGTGCCATTCATAACAGATTCTCCAGAGGTAATGTAGGGATTGTTTTAATTCCCGCTCCCTTATGGGAGAAGGTTAACGCTCGGGTAACCCTTGCCGAATGTAGGCCGGATAAGGCg                                               >  2:2107049/1‑150 (MQ=255)
                                                                                                        aaaTGCGCATTGTCTTCTATCCGCTGATAAATAGTGCCATTCATCACAGATTCTCCAGAGGTAATGTAGGGATTGTTTTAATTCCCGCTCCCTTATGGGAGAAGGTTAACGCTCGGGTAACCCTTGCCGAATGTAGGCCGGATAAGGCGt                                              <  1:209947/150‑1 (MQ=255)
                                                                                                        aaaTGCGCATTGTCTTCTATCCGCTGATAAATAGTGCCATTCATAACAGATTCTCCAGAGGTAATGTAGGGATTGTTTTAATTCCCGCTCCCTTATGGGAGAAGGTTAACGCTCGGGTAACCCTTGCCGAATGTAGGCCGGATAAGGCGt                                              >  1:537009/1‑150 (MQ=255)
                                                                                                         aaTGCGCATTGTCTTCTATCCGCTGATAAATAGTGCCATTCATAACAGATTCTCCAGAGGTAATGTAGGGATTGTTTTAATTCCCGCTCCCTTATGGGAGAAGGTTAACGCTCGGGTAACCCTTGCCGAATGTAGGCCGGATAAGGCGtt                                             <  2:3732284/150‑1 (MQ=255)
                                                                                                         aaTGCGCATTGTCTTCTATCCGCTGATAAATAGTGCCATTCATAACAGATTCTCCAGAGGTAATGTAGGGATTGTTTTAATTCCCGCTCCCTTATGGGAGAAGGTTAACGCTCGGGTAACCCTTGCCGAATGTAGGCCGGATAAGGCGtt                                             <  1:2988222/150‑1 (MQ=255)
                                                                                                             cgcATTGTCTTCTATCCGCTGATAAATAGTGCCATTCATCACAGATTCTCCAGAGGTAATGTAGGGATTGTTTTAATTCCCGCTCCCTTATGGGAGAAGGTTAACGCTCGGGTAACCCTTGCCGAATGTAGGCCGGATAAGGCGTTTAcg                                         >  2:1446910/1‑150 (MQ=255)
                                                                                                                  tGTCTTCTATCCGCTGATAAATAGTGCCATTCATAACAGATTCTCCAGAGGTAATGTAGGGATTGTTTTAATTCCCGCTCCCTTATGGGAGAAGGTTAACGCTCGGGTAACCCTTGCCGAATGTAGGCCGGATAAGGCGTTTACGCCGCa                                    >  2:3001256/1‑150 (MQ=255)
                                                                                                                    tcttctATCCGCTTATAAATAGTGCCATTCATCACAGATTCTCCAGAGGTAATGTAGGGATTGTTTTAATTCCCGCTCCCTTATGGGAGAAGGTTAACGCTCGGGTAACCCTTGCCGAATGTAGGCCGGATAAGGCGTTTACGCCGCATc                                  >  1:3190214/1‑150 (MQ=255)
                                                                                                                     cttctATCCGCTGATAAATAGTGCCATTCATAACAGATTCTCCAGAGGTAATGTAGGGATTGTTTTAATTCCCGCTCCCTTATGGGAGAAGGTTAACGCTCGGGTAACCCTTGCCGAATGTAGGCCGGATAAGGCGTTTACGCCGCATcc                                 >  1:262208/1‑150 (MQ=255)
                                                                                                                     cgtctATCCGCTGATAAATAGTGCCATTCATAACAGATTCTCCAGAGGTAATGTAGGGATTGTTTTAATTCCCGCTCCCTTATGGGAGAAGGTTAACGCTCGGGTAACCCTTGCCGAATGTAGGCCGGATAAGGCGTTTACGCCGCATcc                                 <  2:3518610/148‑1 (MQ=255)
                                                                                                                      ttctATCCGCTGATAAATAGTGCCATTCATAACAGATTCTCCAGAGGTAATGTAGGGATTGTTTTAATTCCCGCTCCCTTATGGGAGAAGGTTAACGCTCGGGTAACCCTTGCCGAATGTAGGCCGGATAAGGCGTTTACGCCGCATCCg                                >  1:942970/1‑150 (MQ=255)
                                                                                                                        ctATCCGCTGATAAATAGTGCCATTCAAAACAGATTCTCCAGAGGTAAAGTAGGGATTGTTATAATTCACGCTCCCTGATGGGAGAAGGTTAACGCTCGGTTAACCCTAGCCGAATGTAGGCCGGATAAGCCGTTAACGCCGCATCCGGc                              >  1:2669988/1‑150 (MQ=255)
                                                                                                                         tATCCGATTATAATTAGTTCAATTCATCACATATTCTCCAGAGGTAATTTAGTTTTTTTTTTAATTCCCGCTCCCTTATGGGAGATGGTTAACGCTCGTGTAACCCTTGCATAATTTAGGCCGGTTAAGGCTTTTACGCCGCATCCGGca                             <  2:1519112/150‑1 (MQ=255)
                                                                                                                          aTCCGCTTATAAATAGTGCCATTCATCACAGATTCTCCAGAGGTAATGTAGGGATTGTTTTAATTCCCGCTCCCTTATGGGAGAAGGTTAACGCTCGGGTAACCCTTGCCGAATGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGcaa                            >  2:2172953/1‑150 (MQ=255)
                                                                                                                           tCCGCTTATAAATAGTGCCATTCATCACAGATTCTCCAGAGGTAATGTAGGGATTGTTTTAATTCCCGCTCCCTTATGGGAGAAGGTTAACGCTCGGGTAACCCTTGCCGAATGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGcaat                           >  1:3644959/1‑150 (MQ=255)
                                                                                                                                tGATAAATAGTGCCATTCATCACAGATTCTCCAGAGGTAATGTAGGGATTGTTTTAATTCCCGCTCCCTTATGGGAGAAGGTTAACGCTCGGGTAACCCTTGCCGAATGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATCAATg                      <  1:2393136/150‑1 (MQ=255)
                                                                                                                                tGATAAATAGTGCCATTCATCACAGATTCTCCAGAGGTAATGTAGGGATTGTTTTAATTCCCGCTCCCTTATGGGAGAAGGTTAACGCTCGGGTAACCCTTGCCGAATGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATCAATg                      <  2:3881757/150‑1 (MQ=255)
                                                                                                                                   tAAATAGTGCCATTCATAACAGATTCTCCAGAGGTAATGTAGGGATTGTTTTAATTCCCGCTCCCTTATGGGAGAAGGTTAACGCTCGGGTAACCCTTGCCGAATGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATCAATGCCt                   <  1:1128967/150‑1 (MQ=255)
                                                                                                                                    aaaTAGTGCCATTCATAACAGATTCTCCAGAGGTAATGTAGGGATTGTTTTAATTCCCGCTCCCTGATGGGAGAAGGTTAACGCTCGGGTAACCCTTGCCGAATGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATCAATGCCTg                  >  2:506817/1‑150 (MQ=255)
                                                                                                                                       tAGTGCCATTCATCACAGATTCTCCAGAGGTAATGTAGGGATTGTTTTAATTCCCGCTCCCTTATGGGAGAAGGTTAACGCTCGGGTAACCCTTGCCGAATGTAGGCCGGATTAGGCGTTTACGCCGCATCCGGCAATCAATGCCTGATg               >  2:2767019/1‑150 (MQ=255)
                                                                                                                                       tAGTGCCATTCATCACAGATTCTCCAGAGGTAATGTAGGGATTGTTTTAATTCCCGCTCCCTTATGGGAGAAGGTTAACGCTCGGGTAACCCTTGCCGAATGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATCAATGCCTGATg               >  2:170568/1‑150 (MQ=255)
                                                                                                                                       tAGTGCCATTCATAACAGATTCTCCAGAGGTAATGTAGGGATTGTTTTAATTCCCGCTCCCTTATGGGAGAAGGTTAACGCTCGGGTAACCCTTGCCGAATGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATCAATGCCTGATg               <  2:734907/150‑1 (MQ=255)
                                                                                                                                            ccATTCATCACAGATTCTCCAGAGGTAATGTAGGGATTGTTTTAATTCCCGCTCCCTTATGGGAGAAGGTTAACGCTCGGGTAACCCTTGCCGAATGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATCAATGCCTGATGCGACg          <  1:1855159/150‑1 (MQ=255)
                                                                                                                                            ccATTCATAACAGATTCTCCAGAGGTAATGTAGGGATTGTTTTAAGTCCCGCTCCCTTCTGGGAGAAGGTTAACGCTCGGGTAACCCTTGCCGAATGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATCAATGCCTGATGCGACg          <  2:1832565/150‑1 (MQ=255)
                                                                                                                                             cATTCATAACAGATTCTCCAGAGGTAATGTAGGGATTGTTTTAATTCCCGCTCCCTTATGGGAGAAGGTTAACGCTCGGGTAACCCTTGCCGAATGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATCAATGCCTGATGCGACGc         >  2:838715/1‑150 (MQ=255)
                                                                                                                                              aTTCATAACAGATTCTCCAGAGGTAATGTAGGGATTGTTTTAATTCCCGCTCCCTTATGGGAGAAGGTTAACGCTCGGGTAACCCTTGCCGAATGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATCAATGCCTGATGCGACGCt        <  1:2556388/150‑1 (MQ=255)
                                                                                                                                              aTTCATAACAGATTCTCCAGAGGTAATGTAGGGATTGTTTTAATTCCCGCTCCCTTATGGGAGAAGGTTAACGCTCGGGTAACCCTTGCCGAATGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATCAATGCCTGATGCGACGCt        <  2:2698686/150‑1 (MQ=255)
                                                                                                                                              aTTCATAACAGAGTCTCCAGAGGTACTGTAGGGATTGTTTTAATTCCCGCTCCCTTATGGGAGAAGGTTAACGCTCGGGTAACCCTTGCCGAATGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATCAATGCCTGATGCGACGCt        <  2:4532834/150‑1 (MQ=255)
                                                                                                                                               ttCATCACAGATTCTCCAGAGGTAATGTAGGGATTGTTTTAATTCCCGCTCCCTTATGGGAGAAGGTTAACGCTCGGGTAACCCTTGCCGAATGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATCAATGCCTGATGCGACGCTg       >  1:626301/1‑150 (MQ=255)
                                                                                                                                                 cataaCAGATTCTCCAGAGGTAATGTAGGGATTGTTTTAATTCCCGCTCCCTTATGGGAGAAGGTTAACGCTCGGGTAACCCTTGCCGAATGTAGGCCGGATAAGGCGTTTACGCCGCATCCAGCAATCAATGCCTGATGCGACGCTGTc     <  2:2234874/150‑1 (MQ=255)
                                                                                                                                                  atctCAGATTCTCCAGAGGTAATGTAGGGATTGTTTTAATTCCCGCTCCCTTATGGGAGAAGGTTAACGCTCGGGTAACCCTTGCCGAATGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTcg    >  1:858183/1‑150 (MQ=255)
                                                                                                                                                  atcaCAGATTCTCCAGAGGTAATGTAGGGATTGTTTTAATTCCCGCTCCCTTATGGGAGAAGGTTAACGCTCGGGTAACCCTTGCCGAATGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTcg    >  2:2952498/1‑150 (MQ=255)
                                                                                                                                                  atcaCAGATTCTCCAGAGGTAATGTAGGGATTGTTTTAATTCCCGCTCCCTTATGGGAGAAGGTTAACGCTCGGGTAACCCTTGCCGAATGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTcg    >  1:4021697/1‑150 (MQ=255)
                                                                                                                                                    cacaGATTCTCCAGAGGTAATGTAGGGATTGTTTTAATTCCCGCTCCCTTATGGGAGAAGGTTAACGCTCGGGTAACCCTTGCCGAATGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTcgcg  >  1:3325961/1‑150 (MQ=255)
                                                                                                                                                    |                                                                                                                                                     
AGCCGGGCGCGAAGGCGATCAGTAAAATAAAGCCGATATAAACTGCCAGCATAATAATCGACAGGATGGTGGCAAACCGTTGCCGTTTTTCGACTAACTCCCTGAAATGCGCATTGTCTTCTATCCGCTGATAAATAGTGCCATTCATCACAGATTCTCCAGAGGTAATGTAGGGATTGTTTTAATTCCCGCTCCCTTATGGGAGAAGGTTAACGCTCGGGTAACCCTTGCCGAATGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCG  >  CP009273/4274994‑4275291

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑