BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	4,297,485	G→C	7.3%	G44G (GGC→GGG)	alsK ←	D‑allose kinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	4,297,485	0	G	C	7.3%	1065.0 / 69.6	287	G44G (GGC→GGG)	alsK	D‑allose kinase
Reads supporting (aligned to +/- strand): ref base G (131/135); new base C (8/13); total (139/148)
Fisher's exact test for biased strand distribution p-value = 3.70e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.24e-01

Only 100 of 294 total aligned reads displayed.

TCCGCCGCTGTTAACGGCAGGTTAGGCGTAGAAATAATGGTGCGTTTATCTTTACTGACCAGCGCCGGAAATCCCATCACCAGACCATGACAGCGAGCGTTAAAGCGCCTGAGTTGCTCGTCAATCATTTCGCCGATACCCGACACCAGGCCGGGAGCAATGACTTCTGCGGTCCGC‑TTTTTTTCGCAGTGTAGCGTTTCACCTTCTGCTGTCCGCAGACAAAAGCGGATATGCGTTGCCCCCATATCCACGCCCGCTACGACGTTATGCTGTTTTTGCATGAGGCTGTACCTCGCTTT  >  CP009273/4297336‑4297634

tCCGCCGCTGTTAACGGCAGGTTAGGCGTAGAAATAATGGTGCGTTTATCTTTACTGACCAGCGCCGGAAATCCCATCACCAGACCATGACAGCGAGCGTTAAAGCGCCTGAGTTGCTCGTCAATCATTTCGCCGATACCCGACACCAgg                                                                                                                                                        <  2:4416595/150‑1 (MQ=255)

   gccgcTGTTAACGGCAGGTTAGGCGTAGAAATAATGGTGCGTTTATCTTTACTGACCAGCGCCGGAAATCCCATCACCAGACCATGACAGCGAGCGTTAAAGCGCCTGAGTTGCTCGTCAATCATTTCGCCGATACCCGACACCAGGCCg                                                                                                                                                     >  2:4045001/1‑150 (MQ=255)

   gccgcTGTTAACGGCAGGTTAGGCGTAGAAATAATGGTGCGTTTATCTTTACTGACCAGCGCCGGAAATCCCATCACCAGACCATGACAGCGAGCGTTAAAGCGCCTGAGTTGCTCGTCAATCATTTCGCCGATACCCGACACCAGGCCg                                                                                                                                                     >  2:2683428/1‑150 (MQ=255)

   gccgcTGTTAACGGCAGGTTAGGCGTAGAAATAATGGTGCGTTTATCTTTACTGACCAGCGCCGGAAATCCCATCACCAGACCATGACAGCGAGCGTTAAAGCGCCTGAGTTGCTCGTCAATCATTTCGCCGATACCCGACACCAGGCCg                                                                                                                                                     <  1:3684477/150‑1 (MQ=255)

     cgcTGTTAACGGCAGGTTAGGCGTAGAAATAATGGTGCGTTTATCTTTACTGACCAGCGCCGGAAATCCCATCACCAGACCATGACAGCGAGCGTTAAAGCGCCTGAGTTGCTCGTCAATCATTTCGCCGATACCCGACACCAGGCCggg                                                                                                                                                   >  1:4324265/1‑150 (MQ=255)

       cTGTTAACGGCAGGTTAGGCGTAGAAATAATGGTGCGTTTATCTTTACTGACCAGCGCCGGAAATCCCATCACCAGACCCTGACAGCGAGCGTTAAAGCGCCTGAGTTGCTCGTCAATCATTTCGCCGATACCCGACACCAGGCCGGGAg                                                                                                                                                 <  1:901542/150‑1 (MQ=255)

       cTGTTAACGGCAGGTTAGGCGTAGAAATAATGGTGCGTTTATCTTTACTGACCAGCGCCGGAAATCCCATCACCAGACCATGACAGCGAGCGTTAAAGCGCCTGAGTTGCTCGTCAATCATTTCGCCGATACCCGACACCAGGCCGGGAg                                                                                                                                                 >  1:2835456/1‑150 (MQ=255)

         gTTAACGGCAGGTTAGGCTTAGAAATAATGGTGCGTTTATCTTTACTGACCAGCGCCGGAAATCCCATCACCAGACCATGACAGCGAGCGTTAAAGCGCCTGAGTGGCTCGTCAATCATTTCGCCGATACCCGACACCAGGCCGGGAGCa                                                                                                                                               <  2:97642/150‑1 (MQ=255)

         gTTAACGGCAGGTTAGGCGTAGAAATAATGGTGCGTTTATCTTTACTGACCAGCGCCGGAAATCCCATCACCAGACCATGACAGCGAGCGTTAAAGCGCCTGAGTTGCTCGTCAATCATTTCGCCGATACCCGACACCAGGCCGGGAGCa                                                                                                                                               <  2:15835/150‑1 (MQ=255)

         gTTAACGGCAGGTTAGGCGTAGAAATAATGGTGCGTTTATCTTTACTGACCAGCGCCGGAAATCCCATCACCAGACCATGACAGCGAGCGTTAAAGCGCCTGAGTTGCTCGTCAATCATTTCGCCGATACCCGACACCAGGCCGGGAGCa                                                                                                                                               <  1:3856035/150‑1 (MQ=255)

           tAACGGCAGGTTAGGCGTAGAAATAATGGTGCGTTTATCTTTACTGACCAGCGCCGGAAATCCCATCACCAGACCATGACAGCGAGCGTTAAAGCGCCTGAGTTGCTCGTCAATCATTTCGCCGATACCCGACACCAGGCCGGGAGCAAt                                                                                                                                             >  1:3513556/1‑150 (MQ=255)

            aaCGGCAGGTTAGGCGTAGAAATAATGGTGCGTTTATCTTTACTGACCAGCGCCGGAAATCCCATCACCAGACCATGACAGCGAGCGTTAAAGCGCCTGAGTTGCTCGTCAATCATTTCGCCGATACCCGACACCAGGCCGGGAGCAATg                                                                                                                                            >  1:2082439/1‑150 (MQ=255)

              cGGCAGGTTAGGCGTAGAAATAATGGTGCGTTTATCTTTACTGACCAGCGCCGGAAATCCCATCACCAGACCATGACAGCGAGCGTTAAAGCGCCTGAGTTGCTCGTCAATCATTTCGCCGATACCCGACACCAGGCCGGGAGCAATGAc                                                                                                                                          <  2:2206271/150‑1 (MQ=255)

               ggCAGGTTAGGCGTAGAAATAATGGTGCGTTTATCTTTACTGACCAGCGCCGGAAATCCCATCACCAGACCATGACAGCGAGCGTTAAAGCGCCTGAGTTGCTCGTCAATCATTTCGCCGATACCCGACACCAGCCCGGGAGCAATGACt                                                                                                                                         >  1:3162405/1‑150 (MQ=255)

                 cAGGTTAGGCGTAGAAATAATGGTGCGTTTATCTTTACTGACCAGCGCCGGAAATCCCATCACCAGACCATGACAGCGAGCGTTAAAGCGCCTGAGTTGCTCGTCAATCATTTCGCCGATACCCGACACCAGGCCGGGAGCAATGACTTc                                                                                                                                       <  1:406287/150‑1 (MQ=255)

                 cAGGTTAGGCGTAGAAATAATGGTGCGTTTATCTTTACTGACCAGCGCCGGAAATCCCATCACCAGACCATGACAGCGAGCGTTAAAGCGCCTGAGTTGCTCGTCAATCATTTCGCCGATACCCGACACCAGGCCGGGAGCAATGACTTc                                                                                                                                       <  1:3733231/150‑1 (MQ=255)

                 cAGGTTAGGCGTAGAAATAATGGTGCGTTTATCTTTACTGACCAGCGCCGGAAATCCCATCACCAGACCATGACAGCGAGCGTTAAAGCGCCTGAGTTGCTCGTCAATCATTTCGCCGATACCCGACACCAGGCCGGGAGCAATGACTTc                                                                                                                                       >  1:3566470/1‑150 (MQ=255)

                    gTTAGGCGTAGAAATAATGGTGCGTTTATCTTTACTGACCAGCGCCGGAAATCCCATCACCAGACCATGACAGCGAGCGTTAAAGCGCCTGAGTTGCTCGTCAATCATTTCGCCGATACCCGACACCAGGCCGGGAGCAATGACTTCTGc                                                                                                                                    <  1:1048327/150‑1 (MQ=255)

                    gTTAGGCGTAGAAATAATGGTGCGTTTATCTTTACTGACCAGCGCCGGAAATCCCATCACCAGACCATGACAGCGAGCGTTAAAGCGCCTGAGTTGCTCGTCAATCATTTCGCCGATACCCGACACCAGGCCGGGAGCAATGACTTCTGc                                                                                                                                    >  2:3467553/1‑150 (MQ=255)

                      tAGGCGTAGAAATAATGGTGCGTTTATCTTTACTGACCAGCGCCGGAAATCCCATCACCAGACCATGACAGCGAGCGTTAAAGCGCCTGAGTTGCTCGTCAATCATTTCGCCGATACCCGACACCAGGCCGGGAGCAATGACTTCTGCgg                                                                                                                                  >  1:766984/1‑150 (MQ=255)

                       aGGCGTAGAAATAATGGTGCGTTTATCTTTACTGACCAGCGCCGGAAATCCCATCACCAGACCATGACAGCGAGCGTTAAAGCGCCTGAGTTGCTCGTCAATCATTTCGCCGATACCCGACACCAGGCCGGGAGCAATGACTTCTGCGGt                                                                                                                                 >  2:3216875/1‑150 (MQ=255)

                       aGGCGTAGAAATAATGGTGCGTTTATCTTTACTGACCAGCGCCGGAAATCCCATCACCAGACCATGACAGCGAGCGTTAAAGCGCCTGAGTTGCTCGTCAATCATTTCGCCGATACCCGACACCAGGCCGGGAGCAATGACTTCTGCGGt                                                                                                                                 >  1:1965185/1‑150 (MQ=255)

                         gCGTAGAAATAATGGTGCGTTTATCTTTACTGACCAGCGCCGGAAATCCCATCACCAGACCATGACAGCGAGCGTTAAAGCGCCTGAGTTGCTCGTCAATCATTTCGCCGATACCCGACACCAGGCCGGGAGCAATGACTTCTGCGGTcc                                                                                                                               <  2:3690555/150‑1 (MQ=255)

                           gTAGAAATAATGGTGCGTTTATCTTTACTGACCAGCGCCGGAAATCCCATCACCAGACCATGACAGCGAGCGTTAAAGCGCCTGAGTTGCTCGTCAATCATTTCGCCGATACCCGACACCAGGCCGGGAGCAATGACTTCTGCGGTCCGc                                                                                                                             >  2:3088567/1‑150 (MQ=255)

                               aaaTAATGGTGCGTTTATCTTTACTGACCAGCGCCGGAAATCCCATCACCAGACCATGACAGCGAGCGTTAAAGCGCCTGAGTTGCTCGTCAATCATTTCGCCGATACCCGACACCAGGCCGGGAGCAATGACTTCTGCGGTCCGC‑tttt                                                                                                                        <  2:3208821/150‑1 (MQ=255)

                                aattatGGTGCGTTTATCTTTACTGTCCATCGCCGGAAATCCCATCACCAGAACATGACAGCGAGCGTTAAAGCGCCTGAGTTGCTCGTCAATATTTTCGCCGATACCCGACACCAGGCCGGGATCAATGACTTATGCGGTCCGC‑ttttt                                                                                                                       <  1:663602/150‑1 (MQ=255)

                                  taatGGTGCGTTTATCTTTGCTGACCAGCGCCGGAAATCCCATCACCAGACCATGACAGCGAGCGTTAAAGCGCCTGAGTTGCTCGTCAATCATTTCGCCGATACCCGACACCAGGCCGGGAGCAATGACTTCTGCGGTCCGC‑ttttttt                                                                                                                     >  1:190507/1‑150 (MQ=255)

                                  taatGGTGCGTTTATCTTTACTGACCAGCGCCGGAAATCCCATCACCAGACCATGACAGCGAGCGTTAAAGCGCCTGAGTTGCTCGTCAATCATTTCGCCGATACCCGACACCAGGCCGGGAGCAATGACTTCTGCGGTCCGC‑ttttttt                                                                                                                     <  2:909332/150‑1 (MQ=255)

                                  taatGGTGCGTTTATCTTTACTGACCAGCGCCGGAAATCCCATCACCAGACCATGACAGCGAGCGTTAAAGCGCCTGAGTTGCTCGTCAATCATTTAGCCGATACCCGACACAAGGCCGGGAGCAATGACTTCTGCGGTCCGC‑ttttatt                                                                                                                     >  2:1477623/1‑150 (MQ=255)

                                    atGGTGCGTTTATCTTTACTGACCAGCGCCGGAAATCCCATCACCAGACCATGACAGCGAGCGTTAAAGCGCCTGAGTTGCTCGTCAATCATTTCGCCGATACCCGACACCAGGCCGGGAGCAATGACTTCTGCGGTCCGC‑TTTTTTTCg                                                                                                                   >  1:1252188/1‑150 (MQ=255)

                                          cGTTTATCTTTACTGACCAGCGCCGGAAATCCCATCACCAGACCATGACAGCGAGCGTTAAAGCGCCTGAGTTGCTCGTCAATCATTTCGCCGATACCCGACACCAGGCCGGGAGCAATGACTTCTGCGGTCCGC‑TTTTTTTCGCAgtgt                                                                                                             >  2:1636526/1‑150 (MQ=255)

                                           gTTTATCTTTACTGACCAGCGCCGGAAATCCCATCACCAGACCATGACAGCGAGCGTTAAAGCGCCTGAGTTGCTCGTCAATCATTTCGCCGATACCCGACACCAGGCCGGGAGCAATGACTTCTGCGGTCCGC‑TTTTTTTCGCAGTGTa                                                                                                            <  2:1561620/150‑1 (MQ=255)

                                           gTTTATCTTTACTGACCAGCGCCGGAAATCCCATCACCAGACCATGACAGCGAGCGTTAAAGCGCCTGAGTTGCTCGTCAATCATTTCGCCGATACCCGACACCAGGCCGGGAGCAATGACTTCTGCGGTCCGC‑TTTTTTTCGCAGTGTa                                                                                                            <  1:2272210/150‑1 (MQ=255)

                                           gTTTATCTTTACTGACCAGCGCCGGAAATCCCATCACCAGACCATGACAGCGAGCGTTAAAGCGCCTGAGTTGCTCGTCAATCATTTCGCCGATACCCGACACCAGGCCGGGAGCAATGACTTCTGCGGTCCGC‑TTTTTTTCGCAGTGTa                                                                                                            >  2:1268528/1‑150 (MQ=255)

                                            tttATCTTTACTGACCAGCGCCGGAAATCCCATCACCAGACCATGACAGCGAGCGTTAAAGCGCCTGAGTTGCTCGTCAATCATTTCGCCGATACCCGACACCAGGCCGGGAGCAATGACTTCTGCGGTCCGC‑TTTTTTTCGCAGTGTAg                                                                                                           >  1:1396248/1‑150 (MQ=255)

                                                 cTTTACTGACCAGCGCCGGAAATCCCATCACCAGACCATGACAGCGAGCGTTAAAGCGCCTGAGTTGCTCGTCAATCATTTCGCCGATACCCGACACCAGGCCGGGAGCAATGACTTCTGCGGTCCGC‑TTTTTTTCGCAGTGTAGCGttt                                                                                                      <  1:4251214/150‑1 (MQ=255)

                                                       tGACCAGCGCCGGAAATCCCATCACCAGACCATGACAGCGAGCGTTAAAGCGCCTGAGTTGCTCGTCAATCATTTCGCCGATACCCGACACCAGGCCGGGAGCAATGACTTCTGCGGTCCGC‑TTTTTTTCGCAGTGTAGCGTTTCACCtt                                                                                                <  2:2862347/150‑1 (MQ=255)

                                                         aCCAGCGCCGGAAATCCCATCACCAGACCATGACAGTGAGCGTTAAAGCGCCTGAGTTGCTCGTCAATCATTTCGCCGATACCCGACACCAGGCCGGGAGCAATGACTTCTGCGGTCCGC‑TTTTTTTCGCAGTGTAGCGTTTCACCTTct                                                                                              >  1:3458979/1‑150 (MQ=255)

                                                               gcCGGAAATCCCATCACCAGACCATGACAGCGAGCGTTAAAGCGCCTGAGTTGCTCGTCAATCATTTCGCCGATACCCGACACCAGGCCGGGAGCAATGACTTCTGCGGTCCGC‑TTTTTTTCGCAGTGTAGCGTTTCACCTTCTGCTGTc                                                                                        <  2:2071175/150‑1 (MQ=255)

                                                                ccGGAAATCCCATCACCAGACCATGACAGCGAGCGTTAAAGCGCCTGAGTTGCTCGTCAATCATTTCGCCGATACCCGACACCAGGCCGGGAGCAATGACTTCTGCGGTCCGC‑TTTTTTTCGCAGTGTAGCGTTTCACCTTCTGCTGTcc                                                                                       <  2:2351535/150‑1 (MQ=255)

                                                                ccGGAAATCCCATCACCAGACCATGACAGCGAGCGTTAAAGCGCCTGAGTTGCTCGTCAATCATTTCGCCGATACCCGACACCAGGCCGGGAGCAATGACTTCTGCGGTCCGC‑TTTTTTTCGCAGTGTAGCGTTTCACCTCCTGCTGTcc                                                                                       <  2:3181066/150‑1 (MQ=255)

                                                                   gAAATCCCATCACCAGACCATGACAGCGAGCGTTAAAGCGCCTGAGTTGCTCGTCAATCATTTCGCCGATACCCGACACCAGCCCGGGAGCAATGACTTCTGCGGTCCGC‑TTTTTTTCGCAGTGTAGCGTTTCACCTTCTGCTGTCCGCa                                                                                    >  2:1176584/1‑150 (MQ=255)

                                                                      aTCCCATCACCAGCCCATGACAGCGAGCGTTAAAGCGCCTGAGTTGCTCGTCAATCATTTCGCCGATACCCGACACCAGGCCGGGAGCAATGACTTCTGCGGTCCGC‑TTTTTTTCGCAGTGTAGCGTTTCACCTTCTGCTGTCCGCAGAc                                                                                 <  2:3610750/150‑1 (MQ=255)

                                                                      aTCCCATCACCAGACCATGACAGCGAGCGTTAAAGCGCCTGAGTTGCTCGTCAATCATTTCGCCGATACCCGACACCAGCCCGGGAGCAATGACTTCTGCGGTCCGC‑TTTTTTTCGCAGTGTAGCGTTTCACCTTCTGCTGTCCGCAGAc                                                                                 >  1:4182768/1‑150 (MQ=255)

                                                                        cccATCACCAGACCATGACAGCGAGCGTTAAAGCGCCTGAGTTGCTCGTCAATCATTTCGCCGATACCCGACACCAGGCCGGGAGCAATGACTTCTGCGGTCCGC‑TTTTTTTCGCAGTGTAGCGTTTCACCTTCTGCTGTCCGCAGACaa                                                                               >  2:1054203/1‑150 (MQ=255)

                                                                              aCCAGACCATGACAGCGAGCGTTAAAGCGCCTGAGTTGCTCGTCAATCATTTCGCCGATACCCGACACCAGCCCGGGAGCAATGACTTCTGCGGTCCGC‑TTTTTTTCGCAGTGTAGCGTTTCACCTTCTGCTGTCCGCAGACAAAAGCgg                                                                         <  1:3335203/150‑1 (MQ=255)

                                                                                cAGACCATGACAGCGAGCGTTAAAGCGCCTGAGTTGCTCGTCAATCATTTCGCCGATACCCGACACCAGCCCGGGAGCAATGACTTCTGCGGTCCGC‑TTTTTTTCGCAGTGTAGCGTTTCACCTTCTGCTGTCCGCAGACAAAAGCGGat                                                                       <  1:39553/150‑1 (MQ=255)

                                                                                   aCCATGACAGCGAGCGTTAAAGCGCCTGAGTTGCTCGTCAATCATTTCGCCGATACCCGACCCCAGGCCGGGAGCAATGACTTCTGCGGTCCGC‑TTTTTTTCGCAGTGTAGCGTTTCACCTTCTGCTGTCCGCAGACAAAAGCGGATATg                                                                    <  2:689236/150‑1 (MQ=255)

                                                                                   aCCATGACAGCGAGCGTTAAAGCGCCTGAGTTGCTCGTCAATCATTTCGCCGATACCCGACACCAGGCCGGGAGCAATGACTTCTGCGGTCCGC‑TTTTTTTCGCAGTGTAGCGTTTCACCTTCTGCTGTCCGCAGACAAAAGCGGATATg                                                                    >  2:2637552/1‑150 (MQ=255)

                                                                                     cATGACAGCGAGCGTTAAAGCGCCTGAGTTGCTCGTCAATCATTTCGCCGATACCCGACACCAGGCCGGGAGCAATGACTTCTGCGGTCCGC‑TTTTTTTCGCAGTGTAGCGTTTCACCTTCTGCTGTCCGCAGACAAAAGCGGATATGCg                                                                  >  2:600825/1‑150 (MQ=255)

                                                                                        gACAGCGAGCGTTAAAGCGCCTGAGTTGCTCGTCAATCATTTCGCCGATACCCGACACCAGGCCGGGAGCAATGACTTCTGCGGTCCGC‑TTTTTTTCGCAGTGTAGCGTTTCACCTTCTGCTGTCCGCAGACAAAAGCGGATATGCGTTg                                                               <  2:626870/150‑1 (MQ=255)

                                                                                             cgagcgTTAAAGCGCCTGAGTTGCTCGTCAATCATTTCGCCGATACCCGACACCAGGCCGGGAGCAATGACTTCTGCGGTCCGC‑TTTTTTTCGCAGTGTAGCGTTTCACCTTCTGCTGTCCGCAGACAAAAGCGGATATGCGTTGccccc                                                          >  2:4486577/1‑150 (MQ=255)

                                                                                             cgagcgTTAAAGCGCCTGAGTTGCTCGTCAATCATTTCGCCGATACCCGACACCAGGCCGGGAGCAATGACTTCTGCGGTCCGC‑TTTTTTTCGCAGTGTAGCGTTTCACCTTCTGCTGTCCGCAGACAAAAGCGGATATGCGTTGccccc                                                          >  1:3062647/1‑150 (MQ=255)

                                                                                              gagcgTTAAAGCGCCTGAGTTGCTCGTCAATCATTTCGCCGATACCCGACACCAGGCCGGGAGCAATGACTTCTGCGGTCCGC‑TTTTTTTCGCAGTGTAGCGTTTCACCTTCTGCTGTCCGCAGACAAAAGCGGATATGCGTTGCCCCCa                                                         >  1:2986117/1‑150 (MQ=255)

                                                                                                 cgTTAAAGCGCCTGAGTTGCTCGTCAATCATTTCGCCGATACCCGACACCAGGCCGGGAGCAATGACTTCTGCGGTCCGC‑TTTTTTTCGCAGTGTAGCGTTTCTCCTTCTGCTGTCCGCAGACAAAAGCGGATATGCGTTGCCCCCatat                                                      >  2:4452385/1‑150 (MQ=255)

                                                                                                 cgTTAAAGCGCCTGAGTTGCTCGTCAATCATTTCGCCGATACCCGACACCAGGCCGGGAGCAATGACTTCTGCGGTCCGC‑TTTTTTTCGCAGTGTAGCGTTTCACCTTCTGCTGTCCGCAGACAAAAGCGGATATGCGTTGCCCCCatat                                                      >  2:3883865/1‑150 (MQ=255)

                                                                                                 cgTTAAAGCGCCTGAGTTGCTCGTCAATCATTTCGCCGATACCCGACACCAGGCCGGGAGCAATGACTTCTGCGGTCCGC‑TTTTTTTCGCAGTGTAGCGTTTCACCTTCTGCTGTCCGCAGACAAAAGCGGATATGCGTTGCCCCCatat                                                      >  2:2138593/1‑150 (MQ=255)

                                                                                                 cgTTAAAGCGCCTGAGTTGCTCGTCAATCATTTCGCCGATACCCGACACCAGGCCGGGAGCAATGACTTCTGCGGTCCGC‑TTTTTTTCGCAGTGTAGCGTTTCACCTTCTGCTGTCCGCAGACAAAAGCGGATATGCGTTGCCCCCatat                                                      >  2:383646/1‑150 (MQ=255)

                                                                                                 cgTTAAAGCGCCTGAGTTGCTCGTCAATCATTTCGCCGATACCCGACACCAGCCCGGGAGCAATGACTTCTGCGGTCCGC‑TTTTTTTCGCAGTGTAGCGTTTCACCTTCTGCTGTCCGCAGACAAAAGCGGATATGCGTTGCCCCCatat                                                      <  2:1828360/150‑1 (MQ=255)

                                                                                                  gTTAAAGCGCCTGAGTTGCTCGTCAATCATTTCGCCGATACCCGACACCAGGCCGGGAGCAATGACTTCTGCGGTCCGCTTTTTTTTCGCAGTGTAGCGTTTCACCTTCTGCTGTCCGCAGACAAAAGCGGATATGCGTTGCCCCCatat                                                      <  1:2192327/150‑1 (MQ=255)

                                                                                                  gTTAAAGCGCCTGAGTTGCTCGTCAATCATTTCGCCGATACCCGACACCAGGCCGGGAGCAATGACTTCTGCGGTCCGC‑TTTTTTTCGCAGTGTAGCGTTTCACCTTCTGCTGTCCGCAGACAAAAGCGGATATGCGTTGCCCCCATATc                                                     <  2:3122714/150‑1 (MQ=255)

                                                                                                  gTTAAAGCGCCTGAGTTGCTCGTCAATCATTTCGCCGATACCCGACACCAGGCCGGGAGCAATGACTTCTGCGGTCCGC‑TTTTTTTCGCAGTGTAGCGTTTCACCTTCTGCTGTCCGCAGACAAAAGCGGATATGCGTTGCCCCCATATc                                                     >  1:1846257/1‑150 (MQ=255)

                                                                                                   ttAAAGCGCCTGAGTTGCTCGTCAATCATTTCGCCGATACCCGACACCAGGCCGGGAGCAATGACTTCTGCGGTCCGC‑TTTTTTTCGCAGTGTAGCGTTTCACCTTCTGCTGTCCGCAGACAAAAGCGGATATGCGTTGCCCCCATATcc                                                    <  1:1549827/150‑1 (MQ=255)

                                                                                                    tAAAGCGCCTGAGTTGCTCGTCAATCATTTCGCCGATACCCGACACCAGGCCGGGAGCAATGACTTCTGCGGTCCGC‑TTTTTTTCACAGTGTAGCGTTTCACCTTCTGCTGTCCGCAGACAAAAGCGGATATGCGTTGCCCCCATATCCa                                                   >  2:1255853/1‑150 (MQ=255)

                                                                                                     aaaGCGCCTGAGTTGCTCGTCAATCATTTCGCCGATACCCGACACCAGGCCGGGAGCAATGACTTCTGCGGTCCGC‑TTTTTTTCGCAGTGTAGCGTTTCACCTTCTGCTGTCCGCAGACAAAAGCGGATATGCGTTGCCCCCATATCCAc                                                  <  1:3370706/150‑1 (MQ=255)

                                                                                                     aaaGCGCCTGAGTTGCTCGTCAATCATTTCGCCGATACCCGACACCAGGCCGGGAGCAATGACTTCTGCGGTCCGC‑TTTTTTTCGCAGTGTAGCGTTTCACCTTCTGCTGTCCGCAGACAAAAGCGGATATGCGTTGCCCCCATATCCAc                                                  <  1:2684074/150‑1 (MQ=255)

                                                                                                     aaaGCGCCTGAGTTGCTCGTCAATCATTTCGCCGATACCCGACACCAGGCCGGGAGCAATGACTTCTGCGGTCCGC‑TTTTGTTCGCAGTGTAGCGTTTCACCTTCTGCTGTCCGCAGACAAAAGCGGATATGCGTTGCCCCCATATCCAc                                                  <  2:2273277/150‑1 (MQ=255)

                                                                                                       aGCGCCTGAGTTGCTCGTCAATCATTTCGCCGATACCCGACACCAGGCCGGGAGCAATGACTTCTGCGGTCCGC‑TTTTTTTCGCAGTGTAGCGTTTCACCTTCTGCTGTCCGCAGAAAAAAGCGGATATGCGTTGCCCCCATATCCACGc                                                >  2:2998998/1‑150 (MQ=255)

                                                                                                        gcgcCTGAGTTGCTCGTCAATCATTTCGCCGATACCCGACACCAGGCCGGGAGCAATGACTTCTGCGGTCCGC‑TTTTTTTCGCAGTGTAGCGTTTCACCTTCTGCTGTCCGCAGACAAAAGCGGATATGCGTTGCCCCCATATCCACGcc                                               <  1:1792129/150‑1 (MQ=255)

                                                                                                        gcgcCTGAGTTGCTCGTCAATCATTTCGCCGATACCCGACACCAGGCCGGGAGCAATGACTTCTGCGGTCCGC‑TTTTTTTCGCAGTGTAGCGTTTCACCTTCTGCTGTCCGCAGACAAAAGCGGATATGCGTTGCCCCCATATCCACGcc                                               <  1:4288402/150‑1 (MQ=255)

                                                                                                             tGAGTTGCTCGTCAATCATTTCGCCGATACCCGACACCAGGCCGGGAGCAATGACTTCTGCGGTCCGC‑TTTTTTTCGCAGTGTAGCGTTTCACCTTCTGCTGTCCGCAGACAAAAGCGGATATGCGTTGCCCCCATATCCACGCCCGCTa                                          >  2:1616582/1‑150 (MQ=255)

                                                                                                               aGTTGCTCGTCAATCATTTCGCCGATACCCGACACCAGGCCGGGAGCAATGACTTCTGCGGTCCGC‑TTTTTTTCGCAGTGTAGCGTTTCACCTTCTGCTGTCCGCAGACAAAAGCGGATATGCGTTGCCCCCATATCCACGCCCGCTacg                                        <  1:4444080/150‑1 (MQ=255)

                                                                                                                gTTGCTCGTCAATCATTTCGCCGATACCCGACACCAGGCCGGGAGCAATGACTTCTGCGGTCCGC‑TTTTTTTCGCAGTGTAGCGTTTCACCTTCTGCTGTCCGCAGACAAAAGCGGATATGCGTTGCCCCCATATCCACGCCCGCTacga                                       >  2:2167639/1‑150 (MQ=255)

                                                                                                                 ttGCTCGTCAATCATTTCGCCGATACCCGACACCAGGCCGGGAGCAATGACTTCTGCGGTCCGC‑TTTTTTTCGCAGTGTAGCGTTTCACCTTCTGCTGTCCGCAGACAAAAGCGGATATGCGTTGCCCCCATATCCACGCCCGCTacgac                                      <  1:1350917/150‑1 (MQ=255)

                                                                                                                 ttGCTCGTCAATCATTTCGCCGATACCCGACACCAGGCCGGGAGCAATGACTTCTGCGGTCCGC‑TTTTTTTCGCAGTGTAGCGTTTCACCTTCTGCTGTCCGCAGACAAAAGCGGATATGCGTTGCCCCCATATCCACGCCCGCTacgac                                      >  1:4361328/1‑150 (MQ=255)

                                                                                                                  tGCTCGTCAATCATTTCGCCGATACCCGACACCAGGCCGGGAGCAATGACTTCTGCGGTCCGC‑TTTTTTTCGCAGTGTAGCGTTTCACCTTCTGCTGTCCGCAGACAAAAGCGGATATGCGTTGCCCCCATATCCACGCCCGCTacgacg                                     <  2:918653/150‑1 (MQ=255)

                                                                                                                  tGCTCGTCAATCATTTCGCCGATACCCGACACCAGGCCGGGAGCAATGACTTCTGCGGTCCGC‑TTTTTTTCGCAGTGTAGCGTTTCACCTTCTGCTGTCCGCAGACAAAAGCGGATATGCGTTGCCCCCATATCCACGCCCGCTacgacg                                     <  2:3589202/150‑1 (MQ=255)

                                                                                                                    cTCGTCAATCATTTCGCCGATACCCGACACCAGGCCGGGAGCAATGACTTCTGCGGTCCGC‑TTTTTTTCGCAGTGTAGCGTTTCACCTTCTGCTGTCCGCAGACAAAAGCGGATATGCGTTGCCCCCATATCCACGCCCGCTACGACGtt                                   >  1:229195/1‑150 (MQ=255)

                                                                                                                    cTCGTCAATCATTTCGCCGATACCCGACACCAGGCCGGGAGCAATGACTTCTGCGGTCCGC‑TTTTTTTCGCAGTGTAGCGTTTCACCTTCTGCTGTCCGCAGACAAAAGCGGATATGCGTTGCCCCCATATCCACGCCCGCTACGACGtt                                   <  1:2533208/150‑1 (MQ=255)

                                                                                                                    cTCGTCAATCATTTCGCCGATACCCGACACCAGGCCGGGAGCAATGACTTCTGCGGTCCGC‑TTTTTTTCGCAGTGTAGCGTTTCACCTTCTGCTGTCCGCAGACAAAAGCGGATATGCGTTGCCCCCATATCCACGCCCGCTACGACGtt                                   <  1:3646763/150‑1 (MQ=255)

                                                                                                                    cTCGTCAATCATTTCGCCGATACCCGACACCAGCCCGGGAGCAATGACTTCTGCGGTCCGC‑TTTTTTTCGCAGTGTAGCGTTTCACCTTCTGCTGTCCGCAGACAAAAGCGGATATGCGTTGCCCCCATATCCACGCCCGCTACGACGtt                                   >  1:871798/1‑150 (MQ=255)

                                                                                                                         cAATCATTTCGCCGATACCCGACACCAGGCCGGGAGCAATGACTTCTGCGGTCCGC‑TTTTTTTCGCAGTGTAGCGTTTCACCTTCTGCTGTCCGCAGACAAAAGCGGATATGCGTTGCCCCCATATCCACGCCCGCTACGACGGTATGCt                              >  2:3935266/1‑150 (MQ=255)

                                                                                                                          aaTCATTTCGCCGATACCCGACACCAGGCCGGGAGCAATGACTTCTGCGGTCCGC‑TTTTTTTCGCAGTGTAGCGTTTCACCTTCTGCTGTCCGCAGACAAAAGCGGATATGCGTTGCCCCCATATCCACGCCCGCTACGACGTTATGCTg                             <  1:1039044/150‑1 (MQ=255)

                                                                                                                          aaTCATTTCGCCGATACCCGACACCAGGCCGGGAGCAATGACTTCTGCGGTCCGC‑TTTTTTTCGCAGTGTAGCGTTTCACCTTCTGCTGTCCGCAGACAAAAGCGGATATGCGTTGCCCCCATATCCACGCCCGCTACGACGTTATGCTg                             <  1:1624453/150‑1 (MQ=255)

                                                                                                                           aTCATTTCGCCGATACCCGACACCAGGCCGGGAGCAATGACTTCTGCGGTCCGC‑TTTTTTTCGCAGTGTAGCGTTTCACCTTCTGCTGTCCGCAGACAAAAGCGGATATGCGTTGCCCCCATATCCACGCCCGCTACGACGTTATGCTGt                            <  2:1761738/150‑1 (MQ=255)

                                                                                                                            tCATTTCGCCGATACCCGACACCAGGCCGGGAGCAATGACTTCTGCGGTCCGC‑TTTTTTTCGCAGTGTAGCGTTTCACCTTCTGCTGTCCGCAGACAAAAGCGGATATGCGTTGCCCCCATATCCACGCCCGCTACGACGTTATGCTGtt                           <  2:4324065/150‑1 (MQ=255)

                                                                                                                             cATTTCGCCGATACCCGACACCAGGCCGGGAGCAATGACTTCTGCGGTCCGC‑TTTTTTTCGCAGTGTAGCGTTTCACCTTCTGCTGTCCGCAGACAAAAGCGGATATGCGTTGCCCCCATATCCACGCCCGCTACGACGTTATGCTGttt                          >  1:2824347/1‑150 (MQ=255)

                                                                                                                              aTTTCGCCGATACCCGACACCAGGCCGGGAGCAATGACTTCTGCGGTCCGC‑TTTTTTTCGCAGTGTAGCGTTTCACCTTCTGCTGTCCGCAGACAAAAGCGGATATGCGTTGCCCCCATATCCACGCCCGCTACGACGTTATGCTGtttt                         >  2:2143600/1‑150 (MQ=255)

                                                                                                                                  cGCCGATACCCGACACCAGGCCGGGAGCAATGACTTCTGCGGTCCGC‑TTTTTTTCGCAGTGTAGCGTTTCACCTTCTGCTGTCCGCAGACAAAAGCGGATATGCGTTGCCCCCATATCCACGCCCGCTACGACGTTATGCTGTTTTTGCa                     >  2:2507916/1‑150 (MQ=255)

                                                                                                                                      gATACCCGACACCAGGCCGGGAGCAATGACTTCTGCGGTCCGC‑TTTTTTTCGCAGTGTAGCGTTTCACCTTCTGCTGTCCGCAGACAAAAGCGGATATTCGTTGCCCCCATATCCACGCCCGCTACGACGTTATGCTGTTTTTGCATGAg                 >  1:476476/1‑150 (MQ=255)

                                                                                                                                      gATACCCGACACCAGGCCGGGAGCAATGACTTCTGCGGTCCGC‑TTTTTTTCGCAGTGTAGCGTTTCACCTTCTGCTGTCCGCAGACAAAAGCGGATATGCGTTGCCCCCATATCCACGCCCGCTACGACGTTATGCTGTTTTTGCATGAg                 >  1:2580338/1‑150 (MQ=255)

                                                                                                                                      gATACCCGACACCAGGCCGGGAGCAATGACTTCTGCGGTCCGC‑TTTTTTTCGCAGTGTAGCGTTTCACCTTCTGCTGTCCGCAGACAAAAGCGGATATGCGTTGCCCCCATATCCACGCCCGCTACGACGTTATGCTGTTTTTGCATGAg                 <  2:2450633/150‑1 (MQ=255)

                                                                                                                                        tACCCGACACCAGGCCGGGAGCAATGACTTCTGCGGTCCGC‑TTTTTTTCGCAGTGTAGCGTTTCACCTTCTGCTGTCCGCAGACAAAAGCGGATATGCGTTGCCCCCATATCCACGCCCGCTACGACGTTATGCTGTTTTTGCATGAGGc               <  2:2054925/150‑1 (MQ=255)

                                                                                                                                         aCCCGACACCAGGCCGGGAGCAATGACTTCTGCGGTCCGC‑TTTTTTTCGCAGTGTAGCGTTTCACCTTCTGCTGTCCGCAGACAAAAGCGGATATGCGTTGCCCCCATATCCACGCCCGCTACGACGTTATGCTGTTTTTGCATGAGGCt              <  2:190507/150‑1 (MQ=255)

                                                                                                                                            cGACACCAGGCCGGGAGCAATGACTTCTGCGGTCCGC‑TTTTTTTCGCAGTGTAGCGTTTCACCTTCTGCTGTCCGCAGACAAAAGCGGATATGCGTTGCCCCCATATCCACGCCCGCTACGACGTTATGCTGTTTTTGCATGAGGCTGTa           <  1:1187579/150‑1 (MQ=255)

                                                                                                                                             gACACCAGGCCGGGAGCAATGACTTCTGCGGTCCGC‑TTTTTTTCGCAGTGTAGCGTTTCACCTTCTGCTGTCCGCAGACAAAAGCGGATATGCGTTTCCCCCATATACACGCCCGCTACGACGTTATGCTGTTTTTTCATGATGCTGTaa          >  1:2668517/1‑149 (MQ=255)

                                                                                                                                                 ccAGGCCGGGAGCAATGACTTCTGCGGTCCGC‑TTTTTTTCGCAGTGTAGCGTTTCACCTTCTGCTGTCCGCAGACAAAAGCGGATATGCGTTGCCCCCATATCCACGCCCGCTACGACGTTATGCTGTTTTTGCATGAGGCTGTACCTCg      <  1:1459270/150‑1 (MQ=255)

                                                                                                                                                 ccAGGCCGGGAGCAATGACTTCTGCGGTCCGC‑TTTTTTTCGCAGTGTAGCGTTTCACCTTCTGCTGTCCGCAGACAAAAGCGGATATGCGTTGCCCCCATATCCACGCCCGCTACGACGTTATGCTGTTTTTGCATGAGGCTGTACCTCg      >  1:635985/1‑150 (MQ=255)

                                                                                                                                                   aGGCCGGGAGCAATGACTTCTGCGGTCCGC‑TTTTTTTCGCAGTGTAGCGTTTCACCTTCTGCTGTCCGCAGACAAAAGCGGATATGCGTTGCCCCCATATCCACGCCCGCTACGACGTTATGCTGTTTTTGCATGAGGCTGTACCTCGCt    >  1:2351111/1‑150 (MQ=255)

                                                                                                                                                    ggCCGGGAGCAATGACTTCTGCGGTCCGC‑TTTTTTTCGCAGTGTAGCGTTTCACCTTCTGCTGTCCGCAGACAAAAGCGGATATGCGTTGCCCCCATATCCACGCCCGCTACGACGTTATGCTGTTTTTGCATGAGGCTGTACCTCGCtt   <  1:2224751/150‑1 (MQ=255)

TCCGCCGCTGTTAACGGCAGGTTAGGCGTAGAAATAATGGTGCGTTTATCTTTACTGACCAGCGCCGGAAATCCCATCACCAGACCATGACAGCGAGCGTTAAAGCGCCTGAGTTGCTCGTCAATCATTTCGCCGATACCCGACACCAGGCCGGGAGCAATGACTTCTGCGGTCCGC‑TTTTTTTCGCAGTGTAGCGTTTCACCTTCTGCTGTCCGCAGACAAAAGCGGATATGCGTTGCCCCCATATCCACGCCCGCTACGACGTTATGCTGTTTTTGCATGAGGCTGTACCTCGCTTT  >  CP009273/4297336‑4297634

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`