BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	4,357,204	A→T	6.5%	L314Q (CTG→CAG)	aspA ←	aspartate ammonia‑lyase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	4,357,204	0	A	T	6.5%	947.3 / 34.9	284	L314Q (CTG→CAG)	aspA	aspartate ammonia‑lyase
Reads supporting (aligned to +/- strand): ref base A (145/118); new base T (7/12); total (152/132)
Fisher's exact test for biased strand distribution p-value = 1.54e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 289 total aligned reads displayed.

TAACGTTCAACTGCAGCTGACCTGCTTCTGCTGCCATGGTAACAGTGGTGTCGTTACCGATGACTTTGAAGCATACCTGGTTAACCACTTCCGGAACAACCGGGTTTACTTTAGCTGGCATGATGGAAGAGCCCGCCTGCAGTTCCGGCAGGTTGATCTCGTTCAGGCCGGCACGTGGGCCTGAAGAGAGCAAGCGCAGGTCGTTACAGATTTTGGACATCTTCACAGCCAGGCGTTTCAGCGCGCCGTGAACCATAACATAAGCGCCGCAGTCAGAGGTCGCTTCGATCAGGTCTTCA  >  CP009273/4357055‑4357353

    gTTCAACTGCAGCTGACCTGCTTCTGCTGCCATGGTAACAGTGGTGTCGTTACCGATGACTTTGAAGCATACCTGGTTAACCACTTCCGGAACAACCGGGTTTACTTTAGCTGGCATGATGGAAGAGCCCGCCTGCAGTTCCGGCAGGtt                                                                                                                                                   >  2:33363/1‑150 (MQ=255)

    gTTCAACTGCAGCTGACCTGCTTCTGCTGCCATGGTAACAGTGGTGTCGTTACCGATGACTTTGAAGCATACCTGGTTAACCACTTCCGGAACAACCGGGTTTACTTTAGCTGGCATGATGGAAGAGCCCGCCTGCAGTTCCGGCAGGtt                                                                                                                                                   <  2:3194248/150‑1 (MQ=255)

    gTTCAACTGCAGCTGACCTGCTTCTGCTGCCATGGTAACAGTGGTGTCGTTACCGATGACTTTGAAGCATACCTGGTTAACCACTTCCGGAACAACCGGGTTTACTTTAGCTGGCATGATGGAAGAGCCCGCCTGCAGTTCCGGCAGGtt                                                                                                                                                   <  1:3761601/150‑1 (MQ=255)

     ttCAACTGCAGCTGACCTGCTTCTGCTGCCATGGTAACAGTGGTGTCGTTACCGATGACTTTGAAGCATACCTGGTTAACCACTTCCGGAACAACCGGGTTTACTTTAGCTGGCATGATGGAAGAGCCCGCCTGCAGTTCCGGCAGGTTg                                                                                                                                                  >  2:2095577/1‑150 (MQ=255)

       cAACTGCAGCTGACCTGCTTCTGCTGCCATGGTAACAGTGGTGTCGTTACCGATGACTTTGAAGCATACCTGGTTAACCACTTCCGGAACAACCGGGTTTACTTTAGCTGGCATGATGGAAGAGCCCGCCTGCAGTTCCGGCAGGTTGAt                                                                                                                                                <  2:1831703/150‑1 (MQ=255)

        aaCTGCAGCTGACCTGCTTCTGCTGCCATGGTAACAGTGGTGTCGTTACCGATGACTTTGAAGCATACCTGGTTAACCACTTCCGGAACAACCGGGTTTACTTTAGCTGGCATGATGGAAGAGCCCGCCTGCAGTTCCGGCTGGTTGAtc                                                                                                                                               >  1:262066/1‑150 (MQ=255)

        aaCTGCAGCTGACCTGCTTCTGCTGCCATGGTAACAGTGGTGTCGTTACCGATGACTTTGAAGCATACCTGGTTAACCACTTCCGGAACAACCGGGTTTACTTTAGCTGGCATGATGGAAGAGCCCGCCTGCAGTTCCGGCTGGTTGAtc                                                                                                                                               >  1:1383721/1‑150 (MQ=255)

         aCTGCAGCTGACCTGCTTCTGCTGCCATGGTAACAGTGGTGTCGTTACCGATGACTTTGAAGCATACCTGGTTAACCACTTCCGGAACAACCGGGTTTACTTTAGCTGGCATGATGGAAGAGCCCGCCTGCAGTTCCGGCAGGTTGAtct                                                                                                                                              >  2:1058960/1‑150 (MQ=255)

          cTGCAGCTGACCTGCTTCTGCTGCCATGGTAACAGTGGTGTCGTTACCGATGACTTTGAAGCATACCTGGTTAACCACTTCCGGAACAACCGGGTTTACTTTAGCTGGCATGATGGAAGAGCCCGCCTGCAGTTCCGGCAGGTTGAtctc                                                                                                                                             <  1:2722672/150‑1 (MQ=255)

          cTGCAGCTGACCTGCTTCTGCTGCCATGGTAACAGTGGTGTCGTTACCGATGACTTTGAAGCATACCTGGTTAACCACTTCCGGAACAACCGGGTTTACTTTAGCTGGCATGATGGAAGAGCCCGCCTGCAGTTCCGGCAGGTTGAtctc                                                                                                                                             <  2:1209488/150‑1 (MQ=255)

          cTGCAGCTGACCTGCTTCTGCTGCCATGGTAACAGTGGTGTCGTTACCGATGACTTTGAAGCATACCTGGTTAACCACTTCCGGAACAACCGGGTTTACTTTAGCTGGCATGATGGAAGAGCCCGCCTGCAGTTCCGGCAGGTTGAtctc                                                                                                                                             <  1:459724/150‑1 (MQ=255)

             cAGCTGACCTGCTTCTGCTGCCATGGTAACAGTGGTGTCGTTACCGATGACTTTGAAGCATACCTGGTTAACCACTTCCGGAACAACCGGGTTTACTTTAGCTGGCATGATGGAAGAGCCCGCCTGCAGTTCCGGCAGGTTGATCTCGtt                                                                                                                                          <  2:4494568/150‑1 (MQ=255)

               gCTGACCTGCTTCTGCTGCCATGGTAACAGTGGTGTCGTTACCGATGACTTTGAAGCATACCTGGTTAACCACTTCCGGAACAACCGGGTTTACTTTAGCTGGCATGATGGAAGAGCCCGCCTGCAGTTCCGGCAGGTTGATCTCGTTCa                                                                                                                                        <  2:179830/150‑1 (MQ=255)

                 tGACCTGCTTCTGCTGCCATGGTAACAGTGGTGTCGTTACCGATGACTTTGAAGCATACCTGGTTAACCACTTCCGGAACACCCGGGTTTACTTTAGCTGGCATGATGGAAGAGCCCGCCTGCAGTTCCGGCAGGTTGATCTCGTTCAgg                                                                                                                                      >  2:570953/1‑150 (MQ=255)

                   aCCTGCTTCTGCTGCCATGGTAACAGTGGTGTCGTTACCGATGACTTTGAAGCATACCTGGTTAACCACTTCCGGAACAACCGGGTTTACTTTAGCTGGCATGATGGAAGAGCCCGCCTGCAGTTCCGGCAGGTTGATCTCGTTCAGGcc                                                                                                                                    >  1:3956743/1‑150 (MQ=255)

                    ccTGCTTCTGCTGCCATGGTAACAGTGGTGTCGTTACCGATGACTTTGAAGCATACCTGGTTAACCACTTCCGGAACAACCGGGTTTACTTTAGCTGGCATGATGGAAGAGCCCGCCTGCAGTTCCGGCAGGTTGATCTCGTTCAGGCCg                                                                                                                                   >  1:1541680/1‑150 (MQ=255)

                    ccTGCTTCTGCTGCCATGGTAACAGTGGTGTCGTTACCGATGACTTTGAAGCATACCTGGTTAACCACTTCCGGAACAACCGGGTTTACTTTAGCTGGCATGATGGAAGAGCCCGCCTGCAGTTCCGGCAGGTTGATCTCGTTCAGGCCg                                                                                                                                   <  2:4267137/150‑1 (MQ=255)

                    ccTGCTTCTGCTGCCATGGTAACAGTGGTGTCGTTACCGATGACTTTGAAGCATACCTGGTTAACCACTTCCGGAACAACCGGGTTTACTTTAGCTGGCATGATGGAAGAGCCCGCCTGCAGTTCCGGCAGGTTGATCTCGTTCAGGCCg                                                                                                                                   <  1:2293473/150‑1 (MQ=255)

                     cTGCTTCTGCTGCCATGGTAACAGTGGTGTCGTTACCGATGACTTTGAAGCATACCTGGTTAACCACTTCCGGAACAACCGGGTTTACTTTAGCTGGCATGATGGAAGAGCCCGCCTGCAGTTCCGGCAGGTTGATCTCGTTCAGGCCgg                                                                                                                                  >  2:1307418/1‑150 (MQ=255)

                        cTTCTGCTGCCATGGTAACAGTGGTGTCGTTACCGATGACTTTGAAGCATACCTGGTTAACCACTTCCGGAACAACCGGGTTTACTTTAGCTGGCATGATGGAAGAGCCCGCCTGCAGTTCCGGCAGGTTGATCTCGTTCAGGCCGGCAc                                                                                                                               <  1:3620139/150‑1 (MQ=255)

                           ctgctgCCATGGTAACAGTGGTGTCGTTACCGATGACTTTGAAGCATACCTGGTTAACCACTTCCGGAACAACCGGGTTTACTTTAGCTGGCATGATGGAAGAGCCCGCCTGTAGTTCCGGCAGGTTGATCTCGTTCAGGCCGGCACGTg                                                                                                                            >  2:15232/1‑150 (MQ=255)

                            tgctgcCATGGTAACAGTGGTGTCGTTACCGATGACTTTGAAGCATACCTGGTTAACCACTTCCGGAACAACCGGGTTTACTTTAGCTGGCATGATGGAAGAGCCCGCCTGCAGTTCCGGCAGGTTGATCTCGTTCAGGCCGGCACGTgg                                                                                                                           <  2:75673/150‑1 (MQ=255)

                                  cATGGTAACAGTGGTGTCGTTACCGATGACTTTGAAGCATACCTGGTTAACCACGTCCGGAACAACCGGGTTTACTTTAGCTGGCATGATGGAAGAGCCCGCCTGCAGTTCCGGCAGGTTGATCTCGTTCAGGCCGGCACGTGGGCCTGa                                                                                                                     <  2:12725/150‑1 (MQ=255)

                                   aTGGTAACAGTGGTGTCGTTACCGATGACTTTGAAGCATACCTGGTTAACCACTTCCGGAACAACCGGGTTTACTTTAGCTGGCATGATGGAAGAGCCCGCCTGCAGTTCCGGCAGGTTGATCTCGTTCAGGCCGGCACGTGGGCCTGaa                                                                                                                    <  1:216243/150‑1 (MQ=255)

                                   aTGGTAACAGTGGTGTCGTTACCGATGACTTTGAAGCATACCTGGTTAACCACTTCCGGAACAACCGGGTTTACTTTAGCTGGCATGATGGAAGAGCCCGCCTGCAGTTCCGGCAGGTTGATCTCGTTCAGGCCGGCACGTGGGCCTGaa                                                                                                                    >  2:1693176/1‑150 (MQ=255)

                                    tGGTAACAGTGGTGTCGTTACCGATGACTTTGAAGCATACCTGGTTAACCACTTCCGGAACAACCGGGTTTACTTTAGCTGGCATGATGGAAGAGCCCGCCTGCAGTTCCGGCAGGTTGATCTCGTTCAGGCCGGCACGTGGGCCTGAag                                                                                                                   >  1:3419788/1‑150 (MQ=255)

                                    tGGTAACAGTGGTGTCGTTACCGATGACTTTGAAGCATACCTGGTTAACCACTTCCGGAACAACCGGGTTTACTTTAGCTGGCATGATGGAAGAGCCCGCCTGCAGTTCCGGCAGGTTGATCTCGTTCAGGCCGGCACGTGGGCCTGAag                                                                                                                   <  1:1676043/150‑1 (MQ=255)

                                       tAACAGTGGTGTCGTTACCGATGACTTTGAAGCATACCTGGTTAACCACTTCCGGAACAACCGGGTTTACTTTAGCTGGCATGATGGAAGAGCCCGCCTGCAGTTCCGGCAGGTTGATCTCGTTCAGGCCGGCACGTGGGCCTGAagaga                                                                                                                >  2:1737472/1‑150 (MQ=255)

                                       tAACAGTGGTGTCGTTACCGATGACTTTGAAGCATACCTGGTTAACCACTTCCGGAACAACCGGGTTTACTTTAGCTGGCATGATGGAAGAGCCCGCCTGCAGTTCCGGCAGGTTGATCTCGTTCAGGCCGGCACGTGGGCCTGAagaga                                                                                                                >  1:4296064/1‑150 (MQ=255)

                                        aaCAGTGGTGTCGTTACCGATGACTTTGAAGCATACCTGGTTAACCACTTCCGGAACAACCGGGTTTACTTTAGCTGGCATGATGGAAGAGCCCGCCTGCAGTTCCGGCAGGTTGATCTCGTTCAGGCCGGCACGTGGGCCTGAagagag                                                                                                               <  1:3222575/150‑1 (MQ=255)

                                         aCAGTGGTGTCGTTACCGATGACTTTGAAGCATACCTGGTTAACCACTTCCGGAACAACCGGGTTTACTTTAGCTGGCATGATGGAAGAGCCCGCCTGCAGTTCCGGCAGGTTGATCTCGTTCAGGCCGGCACGTGGGCCTGAAGAGAGc                                                                                                              <  2:3963054/150‑1 (MQ=255)

                                            gtggtgTCGTTACCGATGACTTTGAAGCATCCCTGGTTAACCACGTCCGGAACAACCGGGTTTACTTTAGCTGGCATGATGGAAGAGCCCGCCTGCAGTTCCGGCAGGTTGATCTCGTTCAGGCCGGCACGTGGGCCTGAAGAGAGCAAg                                                                                                           <  2:2986340/150‑1 (MQ=255)

                                            gtggtgTCGTTACCGATGACTTTGAAGCATACCTGGTTAACCACTTCCGGAACAACCGGGTTTACTTTAGCTGGCATGATGGAAGAGCCCGCCTGCAGTTCCGGCAGGTTGATCTCGTTCAGGCCGGCACGTGGGCCTGAAGAGAGCAAg                                                                                                           >  1:1459655/1‑150 (MQ=255)

                                             tggtgTCGTTACCGATGACTTTGAAGCATACCTGGTTAACCACTTCCGGAACAACCGGGTTTACTTTAGCTGGCATGATGGAAGAGCCCGCCTGCAGTTCCGGCAGGTTGATCTCGTTCAGGCCGGCACGTGGGCCTGAAGAGAGCAAgc                                                                                                          <  2:4332586/150‑1 (MQ=255)

                                                 gtCGTTACCGATGACTTTGAAGCATACCTGGTTAACCACTTCCGGAACAACCGGGTTTACTTTAGCTGGCATGATGGAAGAGCCCGCCTGCAGTTCCGGCAGGTTGATCTCGTTCAGGCCGGCACGTGGGCCTGAAGAGAGCAAGCGCAg                                                                                                      >  1:4493462/1‑150 (MQ=255)

                                                    gTTACCGATGACTTTGAAGCATACCTGGTTAACCACTTCCGGAACAACCGGGTTTACTTTAGCTGGCATGATGGAAGAGCCCGCCTGCAGTTCCGGCAGGTTGATCTCGTTCAGGCCGGCACGTGGGCCTGAAGAGAGCAAGCGCAGGTc                                                                                                   >  2:4396936/1‑150 (MQ=255)

                                                         cGATGACTTTGAAGCATACCTGGTTAACCACTTCCGGAACAACCGGGTTTACTTTAGCTGGCATGATGGAAGAGCCCGCCTGCAGTTCCGGCAGGTTGATCTCGTTCAGGCCGGCACGTGGGCCTGAAGAGAGCAAGCGCAGGTCGTTAc                                                                                              <  2:1841921/150‑1 (MQ=255)

                                                          gATGACTTTGAAGCATACCTGGTTAACCACTTCCGGAACAACCGGGTTTACTTTAGCTGGCATGATGGAAGAGCCCGCCTGCAGTTCCGGCAGGTTGATCTCGTTCAGGCCGGCACGTGGGCCTGAAGAGAGCAAGCGCAGGTCGTTACa                                                                                             >  2:3084608/1‑150 (MQ=255)

                                                          gATGACTTTGAAGCATACCTGGTTAACCACTTCCGGAACAACCGGGTTTACTTTAGCTGGCATGATGGAAGAGCCCGCCTGCAGTTCCGGCAGGTTGATCTCGTTCAGGCCGGCACGTGGGCCTGAAGAGAGCAAGCGCAGGTCGTTACa                                                                                             >  2:4121198/1‑150 (MQ=255)

                                                           aTGACTTTGAAGCATACCTGGTTAACCACTTCCGGAACAACCGGGTTTACTTTAGCTGGCATGATGGAAGAGCCCGCCTGCAGTTCCGGCAGGTTGATCTCGTTCAGGCCGGCACGTGGGCCTGAAGAGAGCAAGCGCAGGTCGTTACAg                                                                                            >  2:2060109/1‑150 (MQ=255)

                                                             gACTTTGAAGCATACCTGGTTAACCACTTCCGGAACAACCGGGTTTACTTTAGCTGGCATGATGGAAGAGCCCGCCTGCAGTTCCGGCTGGTTGATCTCGTTCAGGCCGGCACGTGGGCCTGAAGAGAGCAAGCGCAGGTCGTTACAGAt                                                                                          <  2:216197/150‑1 (MQ=255)

                                                              aCTTTGAAGCATACCTGGTTAACCACTTCCGGAACAACCGGGTTTACTTTAGCTGGCATGATGGAAGAGCCCGCCTGCAGTTCCGGCTGGTTGATCTCGTTCAGGCCGGCACGTGGGCCTGAAGAGAGCAAGCGCAGGTCGTTACAGAtt                                                                                         >  2:4508552/1‑150 (MQ=255)

                                                                  tGAAGCATACCTGGTTAACCACTTCCGGAACAACCGGGTTTACTTTAGCTGGCATGATGGAAGAGCCCGCCTGCAGTTCCGGCAGGTTGATCTCGTTCAGGCCGGCACGTGGGCCTGAAGAGAGCAAGCGCAGGTCGTTACAGATTTTgg                                                                                     >  1:4444163/1‑150 (MQ=255)

                                                                    aaGCATACCTGGTTAACCACTTCCGGAACAACCGGGTTTACTTTAGCTGGCATGATGGAAGAGCCCGCCTGCAGTTCCGGCTGGTTGATCTCGTTCAGGCCGGCACGTGGGCCTGAAGAGAGCAAGCGCAGGTCGTTACAGATTTTGGAc                                                                                   <  1:3494696/150‑1 (MQ=255)

                                                                      gCATACCTGGTTAACCACTTCCGGAACAACCGGGTTTACTTTAGCTGGCATGATGGAAGAGCCCGCCTGCAGTTCCGGCAGGTTGATCTCGTTCAGGCCGGCACGTGGGCCTGAAGAGAGCAAGCGCAGGTCGTTACAGATTTTGGACAt                                                                                 <  1:917402/150‑1 (MQ=255)

                                                                       cATACCTGGTTAACCACTTCCGGAACAACCGGGTTTACTTTAGCTGGCATGATGGAAGAGCCCGCCTGCAGTTCCGGCAGGTTGATCTCGTTCAGGCCGGCACGTGGGCCTGAAGAGAGCAAGCGCAGGTCGTTACAGATTTTGGACATc                                                                                >  2:835376/1‑150 (MQ=255)

                                                                       cATACCTGGTTAACCACTTCCGGAACAACCGGGTTTACTTTAGCTGGCATGATGGAAGAGCCCGCCTGCAGTTCCGGCAGGTTGATCTCGTTCAGGCCGGCACGTGGGCCTGAAGAGAGCAAGCGCAGGTCGTTACAGATTTTGGACATc                                                                                >  2:2906853/1‑150 (MQ=255)

                                                                        aTACCTGGTTAACCACTTCCGGAACAACCGGGTTTACTTTAGCTGGCATGATGGAAGAGCCCGCCTGCAGTTCCGGCAGGTTGATCTCGTTCAGGCCGGCACGTGGGCCTGAAGAGAGCAAGCGCAGGTCGTTACAGATTTTGGACATCt                                                                               <  1:3428641/150‑1 (MQ=255)

                                                                        aTACCTGGTTAACCACTTCCGGAACAACCGGGTTTACTTTAGCTGGCATGATGGAAGAGCCCGCCTGCAGTTCCGGCAGGTTGATCTCGTTCAGGCCGGCACGTGGGCCTGAAGAGAGCAAGCGCAGGTCGTTACAGATTTTGGACATCt                                                                               <  1:1648128/150‑1 (MQ=255)

                                                                           ccTGGTTAACCACTTCCGGAACAACCGGGTTTACTTTAGCTGGCATGATGGAAGAGCCCGCCTGCAGTTCCGGCAGGTTGATCTCGTTCAGGCCGGCACGTGGGCCTGAAGAGAGCAAGCGCAGGTCGTTACAGATTTTGGACATCTTca                                                                            >  2:2809691/1‑150 (MQ=255)

                                                                            cTGGTTAACCACTTCCGGAACAACCGGGTTTACTTTAGCTGGCATGATGGAAGAGCCCGCCTGCAGTTCCGGCAGGTTGATCTCGTTCAGGCCGGCACGTGGGCCTGAAGAGAGCAAGCGCAGGTCGTTACAGATTTTGGACATCTTcac                                                                           <  1:1979337/150‑1 (MQ=255)

                                                                            cTGGTTAACCACTTCCGGAACAACCGGGTTTACTTTAGCTGGCATGAGGGAAGAGCCCGCCTGCAGTTCCGGCAGGTTGATCTCGTTCAGGCCGGCACGTGGGCCTGAAGAGAGCAAGCGCAGGTCGTTACAGATTTTGGACATCTTcac                                                                           >  2:1879922/1‑150 (MQ=255)

                                                                               gTTAACCACGTCCGGAACAACCGGGTTTACTTTAGCTGGCATGATGGAAGAGCCCGCCTGCAGTTCCGGCAGGTTGATCTCGTTCAGGCCGGCACGTGGGCCTGAAGAGAGCAAGCGCAGGTCGTTACAGATTTTGGACATCTTCACAGc                                                                        <  1:3824272/150‑1 (MQ=255)

                                                                                 tAACCACTTCCGGAACAACCGGGTTTACTTTAGCTGGCATGATGGAAGAGCCCGCCTGCAGTTCCGGCAGGTTGATCTCGTTCAGGCCGGCACGTGGGCCTGAAGAGAGCAAGCGCAGGTCGTTACAGATTTTGGACATCTTCACAGCCa                                                                      <  1:3255734/150‑1 (MQ=255)

                                                                                 tAACCACTTCCGGAACAACCGGGTTTACTTTAGCTGGCATGATGGAAGAGCCCGCCTGCAGTTCCGGCAGGTTGATCTCGTTCAGGCCGGCACGTGGGCCTGAAGAGAGCAAGCGCAGGTCGTTACAGATTTTGGACATCTTCACAGCCa                                                                      >  1:2410867/1‑150 (MQ=255)

                                                                                   aCCACTTCCGGAACAACCGGGTTTACTTTAGCTGGCATGATGGAAGAGCCCGCCTGCAGTTCCGGCAGGTTGATCTCGTTCAGGCCGGCACGTGGGCCTGAAGAGAGCAAGCGCAGGTCGTTACAGATTTTGGACATCTTCACAGCCAgg                                                                    >  1:3934696/1‑150 (MQ=255)

                                                                                        ttCCGGAACAACCGGGTTTACTTTAGCTGGCATGATGGAAGAGCCCGCCTGCAGTTCCGGCAGGTTGATCTCGTTCAGGCCGGCACGTGGGCCTGAAGAGAGCAAGCGCAGGTCGTTACAGATTTTGGACATCTTCACAGCCAGGCGttt                                                               <  2:1154060/150‑1 (MQ=255)

                                                                                          ccGGAACCACCGGGTTTACTTTAGCTGGCATGATGGAAGAGCCCGCCTGCAGTTCCGGCTGGTTGATCTCGTTCAGGCCGGCACGTGGGCCTGAAGAGAGCAAGCGCAGGTCGTTACAGATTTTGGACATCTTCACAGCCAGGCGTTTCa                                                             <  2:3057979/150‑1 (MQ=255)

                                                                                          ccGGAACAACCGGGTTTACTTTAGCTGGCATGATGGAAGAGCCCGCCTGCAGTTCCGGCAGGTTGATCTCGTTCAGGCCGGCACGTGGGCCTGAAGAGAGCAAGCGCAGGTCGTTACAGATTTTGGACATCTTCACAGCCAGGCGTTTCa                                                             <  1:1137369/150‑1 (MQ=255)

                                                                                            ggAACAACCGGGTTTACTTTAGCTGGCATGATGGAAGAGCCCGCCTGCAGTTCCGGCAGGTTGATCTCGTTCAGGCCGGCACGTGGGCCTGAAGAGAGCAAGCGCAGGTCGTTACAGATTTTGGACATCTTCACAGCCAGGCGTTTCAgc                                                           >  1:992825/1‑150 (MQ=255)

                                                                                                 aacCGGGTTTACTTTAGCTGGCATGATGGAAGAGCCCGCCTGCAGTTCCGGCAGGTTGATCTCGTTCAGGCCGGCACGTGGGCCTGAAGAGAGCAAGCGCAGGTCGTTACAGATTTTGGACATCTTCACAGCCAGGCGTTTCAGCGCGcc                                                      >  1:1343894/1‑150 (MQ=255)

                                                                                                 aacCGGGTTTACTTTAGCTGGCATGATGGAAGAGCCCGCCTGCAGTTCCGGCAGGTTGATCTCGTTCAGGCCGGCACGTGGGCCTGAAGAGAGCAAGCGCAGGTCGTTACAGATTTTGGACATCTTCACAGCCAGGCGTTTCAGCGCGcc                                                      >  2:3921865/1‑150 (MQ=255)

                                                                                                  acCGGGTTTACTTTAGCTGGCATGATGGAAGAGCCCGCCTGCAGTTCCGGCAGGTTGATCTCGTTCAGGCCGGCACGTGGGCCTGAAGAGAGCAAGCGCAGGTCGTTACAGATTTTGGACATCTTCACAGCCAGGCGTTTCAGCGCGCCg                                                     >  1:2847408/1‑150 (MQ=255)

                                                                                                   ccGGGTTTACTTTAGCTGGCATGATGGAAGAGCCCGCCTGCAGTTCCGGCAGGTTGATCTCGTTCAGGCCGGCACGTGGGCCTGAAGAGAGCAAGCGCAGGTCGTTACAGATTTTGGACATCTTCACAGCCAGGCGTTTCAGCGCGCCGt                                                    >  1:2595665/1‑150 (MQ=255)

                                                                                                     gggTTTACTTTAGCTGGCATGATGGAAGAGCCCGCCTGCAGTTCCGGCAGGTTGATCTCGTTCAGGCCGGCACGTGGGCCTGAAGAGAGCAAGCGCAGGTCGTTACAGATTTTGGACATCTTCACAGCCAGGCGTTTCAGCGCGCCGTGa                                                  >  1:279269/1‑150 (MQ=255)

                                                                                                     gggTTTACTTTAGCTGGCATGATGGAAGAGCCCGCCTGCAGTTCCGGCAGGTTGATCTCGTTCAGGCCGGCACGTGGGCCTGAAGAGAGCAAGCGCAGGTCGTTACAGATTTTGGACATCTTCACAGCCAGGCGTTTCAGCGCGCCGTGa                                                  >  1:705316/1‑150 (MQ=255)

                                                                                                      ggTTTACTTTAGCTGGCATGATGGAAGAGCCCGCCTGCAGTTCCGGCAGGTTGATCTCGTTCAGGCCGGCACGTGGGCCTGAAGAGAGCAAGCGCAGGTCGTTACAGATTTTGGACATCTTCACAGCCAGGCGTTTCAGCGCGCCGTGaa                                                 >  1:3342237/1‑150 (MQ=255)

                                                                                                      ggTTTACTTTAGCTGGCATGATGGAAGAGCCCGCCTGCAGTTCCGGCAGGTTGATCTCGTTCAGGCCGGCACGTGGGCCTGAAGAGAGCAAGCGCAGGTCGTTACAGATTTTGGACATCTTCACAGCCAGGCGTTTCAGCGCGCCGTGaa                                                 <  1:2062110/150‑1 (MQ=255)

                                                                                                       gTTTACTTTAGCTGGCATGATGGAAGAGCCCGCCTGCAGTTCCGGCAGGTTGATCTCGTTCAGGCCGGCACGTGGGCCTGAAGAGAGCAAGCGCAGGTCGTTACAGATTTTGGACATCTTCACAGCCAGGCGTTTCAGCGCGCCGTGAAc                                                >  2:3415273/1‑150 (MQ=255)

                                                                                                          tACTTTAGCTGGCATGATGGAAGAGCCCGCCTGCAGTTCCGGCAGGTTGATCTCGTTCAGGCCGGCACGTGGGCCTGAAGAGAGCAAGCGCAGGTCGTTACAGATTTTGGACATCTTCACAGCCAGGCGTTTCAGCGCGCCGTGAACcat                                             >  1:3115036/1‑150 (MQ=255)

                                                                                                          tACTTTAGCTGGCATGATGGAAGAGCCCGCCTGCAGTTCCGGCAGGTTGATCTCGTTCAGGCCGGCACGTGGGCCTGAAGAGAGCAAGCGCAGGTCGTTACAGATTTTGGACATCTTCACAGCCAGGCGTTTCAGCGCGCCGTGAACcat                                             <  2:3765101/150‑1 (MQ=255)

                                                                                                              ttAGCTGGCATGATGGAAGAGCCCGCCTGCAGTTCCGGCAGGTTGATCTCGTTCAGGCCGGCACGTGGGCCTGAAGAGAGCAAGCGCAGGTCGTTACAGATTTTGGACATCTTCACAGCCAGGCGTTTCAGCGCGCCGTGAACcataaca                                         <  1:695252/150‑1 (MQ=255)

                                                                                                               tAGCTGGCATGATGGAAGAGCCCGCCTGCAGTTCCGGCAGGTTGATCTCGTTCAGGCCGGCACGTGGGCCTGAAGAGAGCAAGCGCAGGTCGTTACAGATTTTGGACATCTTCACAGCCAGGCGTTTCAGCGCGCCGTGAACcataacat                                        >  1:3189565/1‑150 (MQ=255)

                                                                                                               tAGCTGGCATGATGGAAGAGCCCGCCTGCAGTTCCGGCAGGTTGATCTCGTTCAGGCCGGCACGTGGGCCTGAAGAGAGCAAGCGCAGGTCGTTACAGATTTTGGACATCTTCACAGCCAGGCGTTTCAGCGCGCCGTGAACcataacat                                        >  2:142230/1‑150 (MQ=255)

                                                                                                               tAGCTGGCATGATGGAAGAGCCCGCCTGCAGTTCCGGCAGGTTGATCTCGTTCAGGCCGGCACGTGGGCCTGAAGAGAGCAAGCGCAGGTCGTTACAGATTTTGGACATCTTCACAGCCAGGCGTTTCAGCGCGCCGTGAACcataacat                                        >  1:4126591/1‑150 (MQ=255)

                                                                                                                    ggCATGATGGAAGAGCCCGCCTGCAGTTCCGGCAGGTTGATCTCGTTCAGGCCGGCACGTGGGCCTGAAGAGAGCAAGCGCAGGTCGTTACAGATTTTGGACATCTTCACAGCCAGGCGTTTCAGCGCGCCGTGAACCATAACATAAgcg                                   <  2:2252061/150‑1 (MQ=255)

                                                                                                                    ggCATGATGGAAGAGCCCGCCTGCAGTTCCGGCAGGTTGATCTCGTTCAGGCCGGCACGTGGGCCTGAAGAGAGCAAGCGCAGGTCGTTACAGATTTTGGACATCTTCACAGCCAGGCGTTTCAGCGCGCCGTGAACCATAACATAAgcg                                   >  1:1943663/1‑150 (MQ=255)

                                                                                                                    ggCATGATGGAAGAGCCCGCCTGCAGTTCCGGCAGGTTGATCTCGTTCAGGCCGGCACGTGGGCCTGAAGAGAGCAAGCGCAGGTCGTTACAGATTTTGGACATCTTCACAGCCAGGCGTTTCAGCGCGCCGTGAACCATAACATAAgcg                                   >  1:1080883/1‑150 (MQ=255)

                                                                                                                         gatgGAAGAGCCCGCCTGCAGTTCCGGCAGGTTGATCTCGTTCAGGCCGGCACGTGGGCCTGAAGAGAGCAAGCGCAGGTCGTTACAGATTTTGGACATCTTCACAGCCAGGCGTTTCAGCGCGCCGTGAACCATAACATAAGcgccgcc                              >  1:787161/1‑149 (MQ=255)

                                                                                                                         gatgGAAGAGCCCGCCTGCAGTTCCGGCAGGTTGATCTCGTTCAGGCCGGCACGTGGGCCTGAAGAGAGCAAGCGCAGGTCGTTACAGATTTTGGACATCTTCACAGCCAGGCGTTTCAGCGCGCCGTGAACCATAACATAAGCGCCGCa                              <  2:3891501/150‑1 (MQ=255)

                                                                                                                         gatgGAAGAGCCCGCCTGCAGTACCGGCAGGTTGATCTCGTTCAGGCCGGCACGTGGGCCTGAAGAGAGCAAGCGCAGGTCGTTACAGATTTTGGACATCTTCACAGCCAGGCGTTTCAGCGCGCCGTGAACCATAACATAAGCGCCGCa                              <  2:2783384/150‑1 (MQ=255)

                                                                                                                           tgGAAGAGCCCGCCTGCAGTTCCGGCAGGTTGATCTCGTTCAGGCCGGCACGTGGGCCTGAAGAGAGCAAGCGCAGGTCGTTACAGATTTTGGACATCTTCACAGCCAGGCGTTTCAGCGCGCCGTGAACCATAACATAAGCGCCGCAGt                            >  2:1718610/1‑150 (MQ=255)

                                                                                                                             gAAGAGCCCGCCTGCAGTTCCGGCAGGTTGATCTCGTTCAGGCCGGCACGTGGGCCTGAAGAGAGCAAGCGCAGGTCGTTACAGATTTTGGACATCTTCACAGCCAGGCGTTTCAGCGCGCCGTGAACCATAACATAAGCGCCGCAGTCa                          <  2:4097735/150‑1 (MQ=255)

                                                                                                                               agagCCCGCCTGCTGTTCCGGCAGGTTGATCTCGTTCAGGCCGGCACGTGGGCCTGAAGAGAGCAAGCGCAGGTCGTTACAGATTTTGGACATCTTCACAGCCAGGCGTTTCAGCGCGCCGTTAACCATAACATAAGCGCCGCAGTCaga                        <  1:3009311/150‑1 (MQ=255)

                                                                                                                                gagCCCGCCTGCAGTTCCGGCAGGTTGATCTCGTTCAGGCCGGCACGTGGGCCTGAAGAGAGCAAGCGCAGGTCGTTACAGATTTTGGACATCTTCACAGCCAGGCGTTTCAGCGCGCCGTGAACCATAACATAAGCGCCGCAGTCagag                       <  2:4425759/150‑1 (MQ=255)

                                                                                                                                gagCCCGCCTGCAGTTCCGGCAGGTTGATCTCGTTCAGGCCGGCACGTGGGCCTGAAGAGAGCAAGCGCAGGTCGTTACAGATTTTGGACATCTTCACAGCCAGGCGTTTCAGCGCGCCGTGAACCATAACATAAGCGCCGCAGTCagag                       <  2:1906657/150‑1 (MQ=255)

                                                                                                                                   cccGCCTGCTGTTCGGGCTGGTTGATCTCGTTCTGGCCGGCACGTGGGCCTGAAGAGAGCAAGCGCAGGTCGTTACAGATTTTGGACATCTTCACAGCCAGGGGTTTCAGCGCGCCGTGAACCATAACATAAGCGCCGCAGTCAGAGGTc                    <  1:873890/150‑1 (MQ=255)

                                                                                                                                   cccGCCTGCAGTTCCGGCAGGTTGATCTCGTTCAGGCCGGCACGTGGGCCTGAAGAGAGCAAGCGCAGGTCGTTACAGATTTTGGACATCTTCACAGCCAGGCGTTTCAGCGCGCCGTGAACCATAACATAAGCGCCGCAGTCAGAGGTc                    <  2:3660215/150‑1 (MQ=255)

                                                                                                                                     cGCCTGCAGTTCCGGCAGGTTGATCTCGTTCAGGCCGGCACGTGGGCCTGAAGAGAGCAAGCGCAGGTCGTTACAGATTTTGGACATCTTCACAGCCAGGCGTTTCAGCGCGCCGTGAACCATAACATAAGCGCCGCAGTCAGAGGTCGc                  >  2:2467249/1‑150 (MQ=255)

                                                                                                                                       ccTGCAGTTCCGGCAGGTTGATCTCGTTCAGGCCGGCACGTGGGCCTGAAGAGAGCAAGCGCAGGTCGTTACAGATTTTGGAAATCTTCACAGCCAGGCGTTTCAGCGCGCCGTGAACCATAACATACGCGCCGCAGTCAGAGGTCGCtt                <  1:1271922/150‑1 (MQ=255)

                                                                                                                                       ccTGCAGTGCCGGCCGGTTGATCTCGTTCCGGCCGGCACGTGGGCCTGAAGAGAGCACGCGCAGGTCGTTACCGATTTTGGACATCTTCACAGCCAGGCGTTTCAGCGCGCCGTGAACCATAACATACGCGCCGCAGTCAGAGGTCGCtt                <  1:3019853/150‑1 (MQ=255)

                                                                                                                                         tGCAGTTCCGGCAGGTTGATCTCGTTCAGGCCGGCACGTGGGCCTGAAGAGAGCAAGCGCAGGTCGTTACAGATTTTGGACATCTTCACAGCCAGGCGTTTCAGCGCGCCGTGAACCATAACATAAGCGCCGCAGTCAGAGGTCGCTTCg              <  2:2574073/150‑1 (MQ=255)

                                                                                                                                             gTTCCGGCAGGTTGATCTCGTTCAGGCCGGCACGTGGGCCTGAAGAGAGCAAGCGCAGGTCGTTACAGATTTTGGACATCTTCACAGCCAGGCGTTTCAGCGCGCCGTGAACCATAACATAAGCGCCGCAGTCAGAGGTCGCTTCGATCa          <  1:1104397/150‑1 (MQ=255)

                                                                                                                                                ccGGCTGGTTGATCTCGTTCAGGCCGGCACGTGGGCCTGAAGAGAGCAAGCGCAGGTCGTTACAGATTTTGGACATCTTCACAGCCAGGCGTTTCAGCGCGCCGTGAACCATAACATAAGCGCCGCAGTCAGAGGTCGCTTCGATCAGGt       <  2:261832/150‑1 (MQ=255)

                                                                                                                                                ccGGCTGGTTGATCTCGTTCAGGCCGGCACGTGGGCCTGAAGAGAGCAAGCGCAGGTCGTTACAGATTTTGGACATCTTCACAGCCAGGCGTTTCAGCGCGCCGTGAACCATAACATAAGCGCCGCAGTCAGAGGTCGCTTCGATCAGGt       <  2:1383721/150‑1 (MQ=255)

                                                                                                                                                ccGGCAGGTTGATCTCGTTCAGGCCGGCACGTGGGCCTGAAGAGAGCAAGCGCAGGTCGTTACAGATTTTGGACATCTTCACAGCCAGGCGTTTCAGCGCGCCGTGAACCATAACATAAGCGCCGCAGTCAGAGGTCGCTTCGATCAGGt       <  2:487288/150‑1 (MQ=255)

                                                                                                                                                ccGGCAGGTTGATCTCGTTCAGGCCGGCACGTGGGCCTGAAGAGAGCAAGCGCAGGTCGTTACAGATTTTGGACATCTTCACAGCCAGGCGGTTCAGCGCGCCGTGAACCATAACATAAGCGCCGCAGTCAGAGGTCGCTTCGATCAGGt       >  1:1895611/1‑150 (MQ=255)

                                                                                                                                                  ggCAGGTTGATCTCGTTCAGGCCGGCACGTGGGCCTGAAGAGAGCAAGCGCAGGTCGTTACAGATTTTGGACATCTTCACAGCCAGGCGTTTCAGCGCGCCGTGAACCATAACATAAGCGCCGCAGTCAGAGGTCGCTTCGATCAGGTCt     >  2:177616/1‑150 (MQ=255)

                                                                                                                                                  ggCAGGTTGATCTCGTTCAGGCCGGCACGTGGGCCTGAAGAGAGCAAGCGCAGGTCGTTACAGATTTTGGACATCTTCACAGCCAGGCGTTTCAGCGCGCCGTGAACCATAACATAAGCGCCGCAGTCAGAGGTCGCTTCGATCAGGTCt     >  1:1298800/1‑150 (MQ=255)

                                                                                                                                                     aGGTTGATCTCGTTCAGGCCGGCACGTGGGCCTGAAGAGAGCAAGCGCAGGTCGTTACAGATTTTGGACATCTTCACAGCCAGGCGTTTCAGCGCGCCGTGAACCATAACATAAGCGCCGCAGTCAGAGGTCGCTTCGATCAGGTCTTCa  <  2:2558935/150‑1 (MQ=255)

TAACGTTCAACTGCAGCTGACCTGCTTCTGCTGCCATGGTAACAGTGGTGTCGTTACCGATGACTTTGAAGCATACCTGGTTAACCACTTCCGGAACAACCGGGTTTACTTTAGCTGGCATGATGGAAGAGCCCGCCTGCAGTTCCGGCAGGTTGATCTCGTTCAGGCCGGCACGTGGGCCTGAAGAGAGCAAGCGCAGGTCGTTACAGATTTTGGACATCTTCACAGCCAGGCGTTTCAGCGCGCCGTGAACCATAACATAAGCGCCGCAGTCAGAGGTCGCTTCGATCAGGTCTTCA  >  CP009273/4357055‑4357353

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`