Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 4,372,891 A→T 7.3% A144A (GCA→GCT)  epmA → Elongation Factor P Lys34 lysyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092734,372,8910AT 7.3% 917.6 / 47.1 261A144A (GCA→GCT) epmAElongation Factor P Lys34 lysyltransferase
Reads supporting (aligned to +/- strand):  ref base A (135/107);  new base T (8/11);  total (143/118)
Fisher's exact test for biased strand distribution p-value = 3.39e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.95e-01

Only 100 of 274 total aligned reads displayed.

TGTGCCGCAGCTTCCGTAATGAAGAGATGGGGCGTTATCACAACCCTGAGTTCACTATGCTGGAGTGGTATCGACCGCACTATGATATGTACCGGTTGATGAACGAGGTGGACGATCTCTTACAACAGGTGCTGGACTGCCCGGCAGCAGAAAGCCTTTCTTATCAACAAGCTTTCTTGCGTTATCTGGAAATTGACCCGCTCTCTGCCGACAAAACGCAACTGCGGGAAGTCGCAGCGAAACTGGATTTGAGCAATGTTGCTGATACCGAAGAAGACCGCGACACGCTGCTACAATT  >  CP009273/4372743‑4373040
                                                                                                                                                    |                                                                                                                                                     
tgtgCCGCAGCTTCCGTAATGAAGAGATGGGGCGTTATCACAACCCTGAGTTCACTATGCTGGAGTGGTATCGACCGCACTATGATATGTACCGGTTGATGAACGAGGTGGACGATCTCTTACAACAGGTGCTGGACTGCCCGGcagcag                                                                                                                                                      >  2:1984339/1‑150 (MQ=255)
 gtgCCGCAGCTTCCGTAATGAAGAGATGGGGCGTTATCACAACCCTGAGTTCACTATGCTGGAGTGGTATCGACCGCACTATGATATGTACCGGTTGATGAACGAGGTGGACGATCTCTTACAACAGGTGCTGGACTGCCCGGCAGCAGa                                                                                                                                                     >  1:2578671/1‑150 (MQ=255)
 gtgCCGCAGCTTCCGTAATGAAGAGATGGGGCGTTATCACAACCCTGAGTTCACTATGCTGGAGTGGTATCGACCGCACTATGATATGTACCGGTTGATGAACGAGGTGGACGATCTCTTACAACAGGTGCTGGACTGCCCGGCAGCAGa                                                                                                                                                     >  1:2577997/1‑150 (MQ=255)
  tgCCGCAGCTTCCGTAATGAAGAGATGGGGCGTTATCACAACCCTGAGTTCACTATGCTGGAGTGGTATCGACCGCACTATGATATGTACCGGTTGATGAACGAGGTGGACGATCTCTTACAACAGGTGCTGGACTGCCCGGCAGCAGaa                                                                                                                                                    <  1:3126159/150‑1 (MQ=255)
       cAGCTTCCGTAATGAAGAGATGGGGCGTTATCACAACCCTGAGTTCACTATGCTGGAGTGGTATCGACCGCACTATGATATGTACCGGTTGATGAACGAGGTGGACGATCTCTTACAACAGGTGCTGGACTGCCCGGCAGCAGAAAGCCt                                                                                                                                               <  1:3428284/150‑1 (MQ=255)
        aGCTTCCGTAATGAAGAGATGGGGCGTTATCACAACCCTGAGTTCACTATGCTGGAGTGGTATCGACCGCACTATGATATGTACCGGTTGATGAACGAGGTGGACGATCTCTTACAACAGGTGCTGGACTGCCCGGCAGCAGAAAGCCtt                                                                                                                                              <  1:1983116/150‑1 (MQ=255)
        aGCTTCCGTAATGAAGAGATGGGGCGTTATCACAACCCTGAGTTCACTATGCTGGAGTGGTATCGACCGCACTATGATATGTACCGGTTGATGAACGAGGTGGACGATCTCTTACAACAGGTGCTGGACTGCCCGGCAGCAGAAAGCCtt                                                                                                                                              <  2:184525/150‑1 (MQ=255)
         gCTTCCGTAATGAAGAGATGGGGCGTTATCACAACCCTGAGTTCACTATGCTGGAGTGGTATCGACCGCACTATGATATGTACCGGTTGATGAACGAGGTGGACGATCTCTTACAACAGGTGCTGGACTGCCCGGCAGCAGAAAGCCttt                                                                                                                                             <  1:283631/150‑1 (MQ=255)
         gCTTCCGTAATGAAGAGATGGGGCGTTATCACAACCCTGAGTTCACTATGCTGGAGTGGTATCGACCGCACTATGATATGTACCGGTTGATGAACGAGGTGGACGATCTCTTACAACAGGTGCTGGACTGCCCGGCAGCAGAAAGCCttt                                                                                                                                             >  2:3022536/1‑150 (MQ=255)
         gCTTCCGTAATGAAGAGATGGGGCGTTATCACAACCCTGAGTTCACTATGCTGGAGTGGTATCGACCGCACTATGATATGTACCGGTTGATGAACGAGGTGGACGATCTCTTACAACAGGTGCTGGACTGCCCGGCAGCAGAAAGCCttt                                                                                                                                             <  1:835674/150‑1 (MQ=255)
          cTTCCGTAATGAAGAGATGGGGCGTTATCACAACCCTGAGTTCACTATGCTGGAGTGGTATCGACCGCACTATGATATGTACCGGTTGATGAACGAGGTGGACGATCTCTTACAACAGGTGCTGGACTGCCCGGCAGCAGAAAGCCTTTc                                                                                                                                            <  2:142957/150‑1 (MQ=255)
          cTTCCGTAATGAAGAGATGGGGCGTTATCACAACCCTGAGTTCACTATGCTGGAGTGGTATCGACCGCACTATGATATGTACCGGTTGATGAACGAGGTGGACGATCTCTTACAACAGGTGCTGGACTGCCCGGCAGCAGAAAGCCTTTc                                                                                                                                            >  1:1264681/1‑150 (MQ=255)
          cTTCCGTAATGAAGAGATGGGGCGTTATCACAACCCTGAGTTCACTATGCTGGAGTGGTATCGACCGCACTATGATATGTACCGGTTGATGAACGAGGTGGACGATCTCTTACAACAGGTGCTGGACTGCCCGGCAGCAGAAAGCCTTTc                                                                                                                                            >  1:4498119/1‑150 (MQ=255)
           ttCCGTAATGAAGAGATGGGGCGTTATCACAACCCTGAGTTCACTATGCTGGAGTGGTATCGACCGCACTATGATATGTACCGGTTGATGAACGAGGTGGACGATCTCTTACAACAGGTGCTGGACTGCCCGGCAGCAGAAAGCCTTTCt                                                                                                                                           <  1:3407141/150‑1 (MQ=255)
           ttCCGTAATGAAGAGATGGGGCGTTATCACAACCCTGAGTTCACTATGCTGGAGTGGTATCGACCGCACTATGATATGTACCGGTTGATGAACGAGGTGGACGATCTCTTACAACAGGTGCTGGACTGCCCGGCAGCAGAAAGCCTTTCt                                                                                                                                           <  1:1351683/150‑1 (MQ=255)
            tCCGTAATGAAGAGATGGGGCGTTATCACAACCCTGAGTTCACTATGCTGGAGTGGTATCGACCGCACTATGATATGTACCGGTTGATGAACGAGGTGGACGATCTCTTACAACAGGTGCTGGACTGCCCGGCAGCAGAAAGCCTTTCtt                                                                                                                                          <  2:3611647/150‑1 (MQ=255)
                tAATGAAGAGATGGGGCGTTATCACAACCCTGAGTTCACTATGCTGGAGTGGTATCGACCGCACTATGATATGTACCGGTTGATGAACGAGGTGGACGATCTCTTACAACAGGTGCTGGACTGCCCGGCAGCAGAAAGCCTTTCTTATca                                                                                                                                      >  2:3847443/1‑150 (MQ=255)
                 aaTGAAGAGATGGGGCGTTATCACAACCCTGAGTTCACTATGCTGGAGTGGTATCGACCGCACTATGATATGTACCGGTTGATGAACGAGGTGGACGATCTCTTACAACAGGTGCTGGACTGCCCGGCAGCAGAAAGCCTTTCTTATcaa                                                                                                                                     >  2:59829/1‑150 (MQ=255)
                    gAAGAGATGGGGCGTTATCACAACCCTGAGTTCACTATGCTGGAGTGGTATCGACCGCACTATGATATGTACCGGTTGATGAACGAGGTGGACGATCTCTTACAACAGGTGCTGGACTGCCCGGCAGCAGAAAGCCTTTCTTATcaacaa                                                                                                                                  >  2:2973544/1‑150 (MQ=255)
                         gaTGGGGCGTTATCACAACCCTGAGTTCACTATGCTGGAGTGGTATCGACCGCACTATGATATGTACCGGTTGATGAACGAGGTGGACGATCTCTTACAACAGGTGCTGGACTGCCCGGCAGCAGAAAGCCTTTCTTATCAACAAGCttt                                                                                                                             >  1:4352662/1‑150 (MQ=255)
                          aTGGGGCGTTATCACAACCCTGAGTTCACTATGCTGGAGTGGTATCGACCGCACTATGATATGTACCGGTTGATGAACGAGGTGGACGATCTCTTACAACAGGTGCTGGACTGCCCGGCAGCAGAAAGCCTTTCTTATCAACAAGCTTTc                                                                                                                            >  1:4152571/1‑150 (MQ=255)
                                cGTTATCACAACCCTGAGTTCACTATGCTGGAGTGGTATCGACCGCACTATGATATGTACCGGTTGATGAACGAGGTGGACGATCTCTTACAACAGGTGCTGGACTGCCCGGCAGCAGAAAGCCTTTCTTATCAACAAGCTTTCTTGCGt                                                                                                                      >  1:725656/1‑150 (MQ=255)
                                   tATCACAACCCTGAGTTCACTATGCTGGAGTGGTATCGACCGCACTATGATATGTACCGGTTGATGAACGAGGTGGACGATCTCTTACAACAGGTGCTGGACTGCCCGGCAGCAGAAAGCCTTTCTTATCAACACGCTTTCTTGCGTTAt                                                                                                                   <  2:212683/150‑1 (MQ=255)
                                     tCACAACCCTGAGTTCACTATGCTGGAGTGGTATCGACCGCACTATGATATGTACCGGTTGATGAACGAGGTGGACGATCTCTTACAACAGGTGCTGGACTGCCCGGCAGCAGAAAGCCTTTCTTATCAACAAGCTTTCTTGCGTTATCt                                                                                                                 <  1:3721859/150‑1 (MQ=255)
                                     tCACAACCCTGAGTTCACTATGCTGGAGTGGTATCGACCGCACTATGATATGTACCGGTTGATGAACGAGGTGGACGATCTCTTACAACAGGTGCTGGACTGCCCGGCAGCAGAAAGCCTTTCTTATCAACAAGCTTTCTTGCGTTATCt                                                                                                                 >  2:2596565/1‑150 (MQ=255)
                                      cacaACCCTGAGTTCACTATGCTGGAGTGGTATCGACCGCACTATGATATGTACCGGTTGATGAACGAGGTGGACGATCTCTTACAACAGGTGCTGGACTGCCCGGCAGCAGAAAGCCTTTCTTATCAACAAGCTTTCTTGCGTTATCTg                                                                                                                <  2:1239484/150‑1 (MQ=255)
                                      cacaACCCTGAGTTCACTATGCTGGAGTGGTATCGACCGCACTATGATATGTACCGGTTGATGAACGAGGTGGACGATCTCTTACAACAGGTGCTGGACTGCCCGGCAGCAGAAAGCCTTTCTTATCAACAAGCTTTCTTGCGTTATCTg                                                                                                                >  2:966618/1‑150 (MQ=255)
                                       acaACCCTGAGTTCACTATGCTGGAGTGGTATCGACCGCACTATGATATGTACCGGTTGATGAACGAGGTGGACGATCTCTTACAACAGGTGCTGGACTGCCCGGCAGCAGAAAGCCTTTCTTATCAACAAGCTTTCTTGCGTTATCTgg                                                                                                               <  1:2638063/150‑1 (MQ=255)
                                        caACCCTGAGTTCACTATGCTGGAGTGGTATCGACCGCACTATGATATGTACCGGTTGATGAACGAGGTGGACGATCTCTTACAACAGGTGCTGGACTGCCCGGCAGCAGAAAGCCTTTCTTATCAACAAGCTTTCTTGCGTTATCTGGa                                                                                                              >  2:3281452/1‑150 (MQ=255)
                                            ccTGAGTTCACTATGCTGGAGTGGTATCGACCGCACTATGATATGTACCGGTTGATGAACGAGGTGGACGATCTCTTACAACAGGTGCTGGACTGCCCGGCAGCAGAAAGCCTTTCTTATCAACAAGCTTTCTTGCGTTATCTGGAAAtt                                                                                                          <  1:4194734/150‑1 (MQ=255)
                                            ccTGAGTTCACTATGCTGGAGTGGTATCGACCGCACTATGATATGTACCGGTTGATGAACGAGGTGGACGATCTCTTACAACAGGTGCTGGACTGCCCGGCAGCAGAAAGCCTTTCTTATCAACAAGCTTTCTTGCGTTATCTGGAAAtt                                                                                                          >  1:3602292/1‑150 (MQ=255)
                                            ccTGAGTTCACTATGCTGGAGTGGTATCGACCGCACTATGATATGTACCGGTTGATGAACGAGGTGGACGATCTCTTACAACAGGTGCTGGACTGCCCGGCAGCAGAAAGCCTTTCTTATCAACAAGCTTTCTTGCGTTATCTGGAAAtt                                                                                                          <  2:3241453/150‑1 (MQ=255)
                                                 gTTCACTATGCTGGAGTGGTATCGACCGCACTATGATATGTACCGGTTGATGAACGAGGTGGACGATCTCTTACAACAGGTGCTGGACTGCCCGGCAGCAGAAAGCCTTTCTTATCAACAAGCTTTCTTGCGTTATCTGGAAATTGAccc                                                                                                     >  1:1948720/1‑150 (MQ=255)
                                                 gTTCACTATGCTGGAGTGGTATCGACCGCACTATGATATGTACCGGTTGATGAACGAGGTGGACGATCTCTTACAACAGGTGCTGGACTGCCCGGCAGCAGAAAGCCTTTCTTATCAACAAGCTTTCTTGCGTTATCTGGAAATTGAccc                                                                                                     >  1:1949772/1‑150 (MQ=255)
                                                  ttCACTATGCTGGAGTGGTATCGACCGCACTATGATATGTACCGGTTGATGAACGAGGTGGACGATCTCTTACAACAGGTGCTGGACTGCCCGGCAGCAGAAAGCCTTTCTTATCAACAAGCTTTCTTGCGTTATCTGGAAATTGACCCg                                                                                                    >  1:1055128/1‑150 (MQ=255)
                                                    cACTATGCTGGAGTGGTATCGACCGCACTATGATATGTACCGGTTGATGAACGAGGTGGACGATCTCTTACAACAGGTGCTGGACTGCCCGGCAGCAGAAAGCCTTTCTTATCAACAAGCTTTCTTGCGTTATCTGGAAATTGACCCGct                                                                                                  <  2:4134901/150‑1 (MQ=255)
                                                       tATGCTGGAGTGGTATCGACCGCACTATGATATGTACCGGTTGATGAACGAGGTGGACGATCTCTTACAACAGGTGCTGGACTGCCCGGCAGCAGAAAGCCTTTCTTATCAACAAGCTTTCTTGCGTTATCTGGAAATTGACCCGctctc                                                                                               >  2:2748038/1‑150 (MQ=255)
                                                       tATGCTGGAGTGGTATCGACCGCACTATGATATGTACCGGTTGATGAACGAGGTGGACGATCTCTTACAACAGGTGCTGGACTGCCCGGCAGCAGAAAGCCTTTCTTATCAACAAGCTTTCTTGCGTTATCTGGAAATTGACCCGctctc                                                                                               >  1:2295237/1‑150 (MQ=255)
                                                       tATGCTGGAGTGGTATCGACCGCACTATGATATGTACCGGTTGATGAACGAGGTGGACGATCTCTTACAACAGGTGCTGGACTGCCCGGCAGCAGAAAGCCTTTCTTATCAACAAGCTTTCTTGCGTTATCTGGAAATTGACCCGctctc                                                                                               >  2:3696382/1‑150 (MQ=255)
                                                       tATGCTGGAGTGGTATCGACCGCACTATGATATGTACCGGTTGATGAACGAGGTGGACGATCTCTTACAACAGGTGCTGGACTGCCCGGCAGCAGAAAGCCTTTCTTATCAACAAGCTTTCTTGCGTTATCTGGAAATTGACCCGctctc                                                                                               >  2:483928/1‑150 (MQ=255)
                                                           cTGGAGTGGTATCGACCGCACTATGATATGTACCGGTTGATGAACGAGGTGGACGATCTCTTACAACAGGTGCTGGACTGCCCGGCAGCAGAAAGCCTTTCTTATCAACAAGCTTTCTTGCGTTATCTGGAAATTGACCCGCTCTCTGcc                                                                                           <  2:1293259/150‑1 (MQ=255)
                                                             ggAGTGGTATCGACCGCACTATGATATGTACCGGTTGATGAACGAGGTGGACGATCTCTTACAACAGGTGCTGGACTGCCCGGCAGCAGAAAGCCTTTCTTATCAACAAGCTTTCTTGCGTTATCTGGAAATTGACCCGCTCTCTGCCGa                                                                                         >  1:767234/1‑150 (MQ=255)
                                                             ggAGTGGTATCGACCGCACTATGATATGTACCGGTTGATGAACGAGGTGGACGATCTCTTACAACAGGTGCTGGACTGCCCGGCAGCAGAAAGCCTTTCTTATCAACAAGCTTTCTTGCGTTATCTGGAAATTGACCCGCTCTCTGCCGa                                                                                         >  1:1033572/1‑150 (MQ=255)
                                                             ggAGTGGTATCGACCGCACTATGATATGTACCGGTTGATGAACGAGGTGGACGATCTCTTACAACAGGTGCTGGACTGCCCGGCAGCAGAAAGCCTTTCTTATCAACAAGCTTTCTTGCGTTATCTGGAAATTGACCCGCTCTCTGCCGa                                                                                         >  1:4375574/1‑150 (MQ=255)
                                                                 tGGTATCGACCGCACTATGATATGTACCGGTTGATGAACGAGGTGGACGATCTCTTACAACAGGTGCTGGACTGCCCGGCAGCAGAAAGCCTTTCTTATCAACAAGCTTTCTTGCGTTATCTGGAAATTGACCCGCTCTCTGCCGACaaa                                                                                     >  2:4337066/1‑150 (MQ=255)
                                                                 tGGTATCGACCGCACTATGATATGTACCGGTTGATGAACGAGGTGGACGATCTCTTACAACAGGTGCTGGACTGCCCGGCAGCAGAAAGCCTTTCTTATCAACAAGCTTTCTTGCGTTATCTGGAAATTGACCCGCTCTCTGCCGACaaa                                                                                     >  2:3689358/1‑150 (MQ=255)
                                                                   gTATCGACCGCACTATGATATGTACCGGTTGATGAACGAGGTGGACGATCTCTTACAACAGGTGCTGGACTGCCCGGCAGCTGAAAGCCTTTCTTATCAACAAGCTTTCTTGCGTTATCTGGAAATTGACCCGCTCTCTGCCGACAAAAc                                                                                   <  1:349280/150‑1 (MQ=255)
                                                                   gTATCGACCGCACTATGATATGTACCGGTTGATGAACGAGGTGGACGATCTCTTACAACAGGTGCTGGACTGCCCGGCAGCAGAAAGCCTTTCTTATCAACAAGCTTTCTTGCGTTATCTGGAAATTGACCCGCTCTCTGCCGACAAAAc                                                                                   >  2:2509692/1‑150 (MQ=255)
                                                                   gTATCGACCGCACTATGATATGTACCGGTTGATGAACGAGGTGGACGATCTCTTACAACAGGTGCTGGACTGCCCGGCAGCAGAAAGCCTTTCTTATCAACAAGCTTTCTTGCGATATCTGGAAATTGACCCGCTCTCTGCCGACAAAAc                                                                                   >  2:3936244/1‑150 (MQ=255)
                                                                      tCGACCGCACTATGATATGTACCGGTTGATGAACGAGGTGGACGATCTCTTACAACAGGTGCTGGACTGCCCGGCAGCAGAAAGCCTTTCTTATCAACAAGCTTTCTTGCGTTATCTGGAAATTGACCCGCTCTCTGCCGACAAAACGCa                                                                                <  1:4025699/150‑1 (MQ=255)
                                                                       cGACCGCACTATGATATGTACCGGTTGATGAACGAGGTGGACGATCTCTTACAACAGGTGCTGGACTGCCCGGCAGCAGAAAGCCTTTCTTATCAACAAGCTTTCTTGCGTTATCTGGAAAGTGACCCGCTCTCTGCCGACAAAACGCaa                                                                               >  2:1682944/1‑150 (MQ=255)
                                                                             cACTATGATATGTACCGGTTGATGAACGAGGTGGACGATCTCTTACAACAGGTGCTGGACTGCCCGGCAGCAGAAAGCCTTTCTTATCAACAAGCTTTCTTGCGTTATCTGGAAATTGACCCGCTCTCTGCCGACAAAACGCAACTGCgg                                                                         >  1:697970/1‑150 (MQ=255)
                                                                             cACTATGATATGTACCGGTTGATGAACGAGGTGGACGATCTCTTACAACAGGTGCTGGACTGCCCGGCAGCAGAAAGCCTTTCTTATCAACAAGCTTTCTTGCGTTATCTGGAAATTGACCCGCTCTCTGCCGACAAAACGCAACTGCgg                                                                         >  1:352033/1‑150 (MQ=255)
                                                                              aCTATGATATGTACCGGTTGATGAACGAGGTGGACGATCTCTTACAACAGGTGCTGGACTGCCCGGCAGCAGAAAGCCTTTCTTATCAACAAGCTTTCTTGCGTTATCTGGAAATTGACCCGCTCTCTGCCGACAAAACGCAACTGCggg                                                                        >  2:2780823/1‑150 (MQ=255)
                                                                              aCTATGATATGTACCGGTTGATGAACGAGGTGGACGATCTCTTACAACAGGTGCTGGACTGCCCGGCAGCAGAAAGCCTTTCTTATCAACAAGCTTTCTTGCGTTATCTGGAAATTGACCCGCTCTCTGCCGACAAAACGCAACTGCggg                                                                        >  2:4131084/1‑150 (MQ=255)
                                                                                tATGATATGTACCGGTTGATGAACGAGGTGGACGATCTCTTACAACAGGTGCTGGACTGCCCGGCAGCAGAAAGCCTTTCTTATCAACAAGCTTTCTTGCGTTATCTGGAAATTGACCCGCTCTCTGCCGACAAAACGCAACTGCGGGaa                                                                      >  1:745887/1‑150 (MQ=255)
                                                                                tATGATATGTACCGGTTGATGAACGAGGTGGACGATCTCTTACAACAGGTGCTGGACTGCCCGGCAGCAGAAAGCCTTTCTTATCAACAAGCTTTCTTGCGTTATCTGGAAATTGACCCGCTCTCTGCCGACAAAACGCAACTGCGGGaa                                                                      >  2:1101922/1‑150 (MQ=255)
                                                                                  tGATATGTACCGGTTGATGAACGAGGTGGACGATCTCTTACAACAGGTGCTGGACTGCCCGGCAGCAGAAAGCCTTTCTTATCAACAAGCTTTCTTGCGTTATCTGGAAATTGACCCGCTCTCTGCCGACAAAACGCAACTGCGGGAAGt                                                                    <  1:2199572/150‑1 (MQ=255)
                                                                                   gATATGTACCGGTTGATGAACGAGGTGGACGATCTCTTACAACAGGTGCTGGACTGCCCGGCAGCAGAAAGCCTTTCTTATCAACAAGCTTTCTTGCGTTATCTGGAAATTGACCCGCTCTCTGCCGACAAAACGCAACTGCGGGAAGTc                                                                   <  1:439944/150‑1 (MQ=255)
                                                                                   gATATGTACCGGTTGATGAACGAGGTGGACGATCTCTTACAACAGGTGCTGGACTGCCCGGCAGCAGAAAGCCTTTCTTATCAACAAGCTTTCTTGCGTTATCTGGAAATTGACCCGCTCTCTGCCGACAAAACGCAACTGCGGGAAGTc                                                                   <  2:2321742/150‑1 (MQ=255)
                                                                                   gATATGTACCGGTTGATGAACGAGGTGGACGATCTCTTACAACAGGTGCTGGACTGCCCGGCAGCAGAAAGCCTTTCTTATCAACAAGCTTTCTTGCGTTATCTGGAAATTGACCCGCTCTCTGCCGACAAAACGCAACTGCGGGAAGTc                                                                   <  1:1739859/150‑1 (MQ=255)
                                                                                    atatGTACCGGTTGATGAACGAGGTGGACGATCTCTTACAACAGGTGCTGGACTGCCCGGCAGCAGAAAGCCTTTCTTATCAACAAGCTTTCTTGCGTTATCTGGAAATTGACCCGCTCTCTGCCGACAAAACGCAACTGCGGGAAGTCg                                                                  <  2:4123658/150‑1 (MQ=255)
                                                                                     tatGTACCGGTTGATGAACGAGGTGGACGATCTCTTACAACAGGTGCTGGACTGCCCGGCAGCTGAAAGCCTTTCTTATCAACAAGCTTTCTTGCGTTATCTGGAAATTGACCCGCTCTCTGCCGACAAAACGCAACTGCGGGAAGTCGc                                                                 <  1:4263992/150‑1 (MQ=255)
                                                                                     tatGTACCGGTTGATGAACGAGGTGGACGATCTCTTACAACAGGTGCTGGACTGCCCGGCAGCAGAAAGCCTTTCTTATCAACAAGCTTTCTTGCGTTATCTGGAAATTGACCCGCTCTCTGCCGACAAAACGCAACTGCGGGAAGTCGc                                                                 >  1:2518658/1‑150 (MQ=255)
                                                                                      atGTACCGGTTGATGAACGAGGTGGACGATCTCTTACAACAGGTGCTGGACTGCCCGGCAGCAGAAAGCCTTTCTTATCAACAAGCTTTCTTGCGTTATCTGGAAATTGACCCGCTCTCTGCCGACAAAACGCAACTGCGGGAAGTCGCa                                                                <  2:2454761/150‑1 (MQ=255)
                                                                                        gTACCGGTTGATGAACGAGGTGGACGATCTCTTACAACAGGTGCTGGACTGCCCGGCAGCAGAAAGCCTTTCTTATCAACAAGCTTTCTTGCGTTATCTGGAAATTGACCCGCTCTCTGCCGACAAAACGCAACTGCGGGAAGTCGCAGc                                                              >  2:1326135/1‑150 (MQ=255)
                                                                                             ggTTGATGAACGAGGTGGACGATCTCTTACAACAGGTGCTGGACTGCCCGGCAGCAGAAAGCCTTTCTTATCAACAAGCTTTCTTGCGTTATCTGGAAATTGACCCGCTCTCTGCCGACAAAACGCAACTGCGGGAAGTCGCAGCGAAAc                                                         >  2:4083189/1‑150 (MQ=255)
                                                                                                tgatgaACGAGGTGGACGATCTCTTACAACAGGTGCTGGACTGCCCGGCAGCAGAAAGCCTTTCTTATCAACAAGCTTTCTTGCGTTATCTGGAAATTGACCCGCTCTCTGCCGACAAAACGCAACTGCGGGAAGTCGCAGCGAAACTgg                                                      <  2:3619016/150‑1 (MQ=255)
                                                                                                     aaCGAGGTGGACGATCTCTTACAACAGGTGCTGGACTGCCCGGCAGCAGAAAGCCTTTCTTATCAACAAGCTTTCTTGCGTTATCTGGAAATTGACCCGCTCTCTGCCGACAAAACGCAACTGCGGGAAGTCGCAGCGAAACTGGATTTg                                                 >  1:2773810/1‑150 (MQ=255)
                                                                                                     aaCGAGGTGGACGATCTCTTACAACAGGTGCTGGACTGCCCGGCAGCAGAAAGCCTTTCTTATCAACAAGCTTTCTTGCGTTATCTGGAAATTGACCCGCTCTCTGCCGACAAAACGCAACTGCGGGAAGTCGCAGCGAAACTGGATTTg                                                 >  1:907214/1‑150 (MQ=255)
                                                                                                     aaCGAGGTGGACGATCTCTTACAACAGGTGCTGGACTGCCCGGCAGCAGAAAGCCTTTCTTATCAACAAGCTTTCTTGCGTTATCTGGAAATTGACCCGCTCTCTGCCGACAAAACGCAACTGCGGGAAGTCGCAGCGAAACTGGATTTg                                                 >  1:508745/1‑150 (MQ=255)
                                                                                                      aCGAGGTGGACGATCTCTTACAACAGGTGCTGGACTGCCCGGCAGCAGAAAGCCTTTCTTATCAACAAGCTTTCTTGCGTTATCTGGAAATTGACCCGCTCTCTGCCGACAAAACGCAACTGCGGGAAGTCGCAGCGAAACTGGATTTGa                                                >  2:1030063/1‑150 (MQ=255)
                                                                                                       cGAGGTGGACGATCTCTTACAACAGGTGCTGGACTGCCCGGCAGCAGAAAGCCTTTCTTATCAACAAGCTTTCTTGCGTTATCTGGAAATTGACCCGCTCTCTGCCGACAAAACGCAACTGCGGGAAGTCGCAGCGAAACTGGATTTGAg                                               <  1:395819/150‑1 (MQ=255)
                                                                                                        gAGGTGGACGATCTCTTACAACAGGTGCTGGACTGCCCGGCAGCAGAAAGCCTTTCTTATCAACAAGCTTTCTTGCGTTATCTGGAAATTGACCCGCTCTCTGCCGACAAAACGCAACTGCGGGAAGTCGCAGCGAAACTGGATTTGAGc                                              >  2:807651/1‑150 (MQ=255)
                                                                                                        gAGGTGGACGATCTCTTACAACAGGTGCTGGACTGCCCGGCAGCAGAAAGCCTTTCTTATCAACAAGCTTTCTTGCGTTATCTGGAAATTGACCCGCTCTCTGCCGACAAAACGCAACTGCGGGAAGTCGCAGCGAAACTGGATTTGAGc                                              >  2:807728/1‑150 (MQ=255)
                                                                                                         aGGTGGACGATCTCTTACAACAGGTGCTGGACTGCCCGGCAGCAGAAAGCCTTTCTTATCAACAAGCTTTCTTGCGTTATCTGGAAATTGACCCGCTCTCTGCCGACAAAACGCAACTGCGGGAAGTCGCAGCGAAACTGGATTTGAGCa                                             >  1:1680694/1‑150 (MQ=255)
                                                                                                            tGGACGATCTCTTACAACAGGTGCTGGACTGCCCGGCAGCAGAAAGCCTTTCTTATCAACAAGCTTTCTTGCGTTATCTGGAAATTGACCCGCTCTCTGCCGACAAAACGCAACTGCGGGAAGTCGCAGCGAAACTGGATTTGAGCAATg                                          <  2:2224760/150‑1 (MQ=255)
                                                                                                             ggACGATCTCTTACAACAGGTGCTGGACTGCCCGGCAGCAGAAAGCCTTTCTTATCAACAAGCTTTCTTGCGTTATCTGGAAATTGACCCGCTCTCTGCCGACAAAACGCAACTGCGGGAAGTCGCAGCGAAACTGGATTTGAGCAATGt                                         >  1:3672996/1‑150 (MQ=255)
                                                                                                              gACGATCTCTTACAACAGGTGCTGGACTGCCCGGCAGCAGAAAGCCTTTCTTATCAACAAGCTTTCTTGCGTTATCTGGAAATTGACCCGCTCTCTGCCGACAAAACGCAACTGCGGGAAGTCGCAGCGAAACTGGATTTGAGCACTGtt                                        >  2:2398300/1‑150 (MQ=255)
                                                                                                              gACGATCTCTTACAACAGGTGCTGGACTGCCCGGCAGCAGAAAGCCTTTCTTATCAACAAGCTTTCTTGCGTTATCTGGAAATTGACCCGCTCTCTGCCGACAAAACGCAACTGCGGGAAGTCGCAGCGAAACTGGATTTGAGCAATGtt                                        <  1:327472/150‑1 (MQ=255)
                                                                                                               aCGATCTCTTACAACAGGTGCTGGACTGCCCGGCAGCAGAAAGCCTTTCTTATCAACAAGCTTTCTTGCGTTATCTGGAAATTGACCCGCTCTCTGCCGACAAAACGCAACTGCGGGAAGTCGCAGCGAAACTGGATTTGAGCAATGTTg                                       <  1:4521029/150‑1 (MQ=255)
                                                                                                               aCGATCTCTTACAACAGGTGCTGGACTGCCCGGCAGCAGAAAGCCTTTCTTATCAACAAGCTTTCTTGCGTTATCTGGAAATTGACCCGCTCTCTGCCGACAAAACGCAACTGCGGGAAGTCGCAGCGAAACTGGATTTGAGCAATGTTg                                       <  2:3478529/150‑1 (MQ=255)
                                                                                                                  aTCTCTTACAACAGGTGCTGGACTGCCCGGCAGCAGAAAGCCTTTCTTATCAACAAGCTTTCTTGCGTTATCTGGAAATTGACCCGCTCTCTGCCGACAAAACGCAACTGCGGGAAGTCGCAGCGAAACTGGATTTGAGCAATGTTGCTg                                    <  1:4015429/150‑1 (MQ=255)
                                                                                                                    ctctTACAACAGGTGCTGGACTGCCCGGCAGCAGAAAGCCTTTCTTATCAACAAGCTTTCTTGCGTTATCTGGAAATTGACCCGCTCTCTGCCGACAAAACGCAACTGCGGGAAGTCGCAGCGAAACTGGATTTGAGCAATGTTGCTGAt                                  >  1:2359573/1‑150 (MQ=255)
                                                                                                                     tctTACAACAGGTGCTGGACTGCCCGGCAGCAGAAAGCCTTTCTTATCAACAAGCTTTCTTGCGTTATCTGGAAATTGACCCGCTCTCTGCCGACAAAACGCAACTGCGGGAAGTCGCAGCGAAACTGGATTTGAGCAATGTTGCTGATa                                 >  1:127906/1‑150 (MQ=255)
                                                                                                                     tctTACAACAGGTGCTGGACTGCCCGGCAGCAGAAAGCCTTTCTTATCAACAAGCTTTCTTGCGTTATCTGGAAATTGACCCGCTCTCTGCCGACAAAACGCAACTGCGGGAAGTCGCAGCGAAACTGGATTTGAGCAATGTTGCTGATa                                 <  1:2708329/150‑1 (MQ=255)
                                                                                                                      ctTACAACAGGTGCTGGACTGCCCGGCAGCAGAAAGCCTTTCTTATCAACAAGCTTTCTTGCGTTATCTGGAAATTGACCCGCTCTCTGCCGACAAAACGCAACTGCGGGAAGTCGCAGCGAAACTGGATTTGAGCAATGTTGCTGATAc                                <  1:1678180/150‑1 (MQ=255)
                                                                                                                       ttACAACAGGTGCTGGACTGCCCGGCAGCAGAAAGCCTTTCTTATCAACAAGCTTTCTTGCGTTATCTGGAAATTGACCCGCTCTCTGCCGACAAAACGCAACTGCGGGAAGTCGCAGCGAAACTGGATTTGAGCAATGTTGCTGATAcc                               <  2:214269/150‑1 (MQ=255)
                                                                                                                          caacaGGTGCTGGACTGCCCGGCAGCAGAAAGCCTTTCTTATCAACAAGCTTTCTTGCGTTATCTGGAAATTGACCCGCTCTCTGCCGACAAAACGCAACTGCGGGAAGTCGCAGCGAAACTGGATTTGAGCAATGTTGCTGATACCgaa                            >  2:927016/1‑150 (MQ=255)
                                                                                                                             caGGTGCTGGACTGCCCGGCAGCTGAAAGCCTTTCTTATCAACAAGCTTTCTTGCGTTATCTGGAAATTGACCCGCTCTCTGCCGACAAAACGCAACTGCGGGAAGTCGCAGCGAAACTGGATTTGAGCAATGTTGCTGATACCgaagaa                         >  2:4416467/1‑150 (MQ=255)
                                                                                                                                gTGCTGGACTGCCCGGCAGCAGAAAGCCTTTCTTATCAACAAGCTTTCTTGCGTTATCTGGAAATTGACCCGCTCTCTGCCGACAAAACGCAACTGCGGGAAGTCGCAGCGAAACTGGATTTGAGCAATGTTGCTGATACCGAAGAAGAc                      <  2:2037545/150‑1 (MQ=255)
                                                                                                                                 tacttgACTGCCCGGCAGCAGAAAGCCTTTCTTATCAACAAGCTTTCTTGCGTTATCTGGAAATTGACCCGCTCTCTGCCGACAAAACGCAACTGCGGGAAGTCGCAGCGAAACTGGATTTGAGCAATGTTGCTGATACCGAAGAAGAcc                     <  1:1068589/145‑1 (MQ=255)
                                                                                                                                   cTGGACTGCCCGGCAGCAGAAAGCCTTTCTTATCAACAAGCTTTCTTGCGTTATCTGGAAATTGACCCGCTCTCTGCCGACAAAACGCAACTGCGGGAAGTCGCAGCGAAACTGGATTTGAGCAATGTTGCTGATACCGAAGAAGACcgc                   >  2:1772908/1‑150 (MQ=255)
                                                                                                                                   cTGGACTGCCCGGCAGCAGAAAGCCTTTCTTATCAACAAGCTTTCTTGCGTTATCTGGAAATTGACCCGCTCTCTGCCGACAAAACGCAACTGCGGGAAGTCGCAGCGAAACTGGATTTGAGCAATGTTGCTGATACCGAAGAAGACcgc                   >  1:3294686/1‑150 (MQ=255)
                                                                                                                                   cTGGACTGCCCGGCAGCAGAAAGCCTTTCTTATCAACAAGCTTTCTTGCGTTATCTGGAAATTGACCCGCTCTCTGCCGACAAAACGCAACTGCGGGAAGTCGCAGCGAAACTGGATTTGAGCAATGTTGCTGATACCGAAGAAGACcgc                   >  1:2955597/1‑150 (MQ=255)
                                                                                                                                    tGGACTGCCCGGCAGCAGAAAGCCTTTCTTATCAACAAGCTTTCTTGCGTTATCTGGAAATTGACCCGCTCTCTGCCGACAAAACGCAACTGCGGGAAGTCGCAGCGAAACTGGATTTGAGCAATGTTGCTGATACCGAAGAAGACcgcg                  >  1:2264592/1‑150 (MQ=255)
                                                                                                                                    tGGACTGCCCGGCAGCAGAAAGCCTTTCTTATCAACAAGCTTTCTTGCGTTATCTGGAAATTGACCCGCTCTCTGCCGACAAAACGCAACTGCGGGAAGTCGCAGCGAAACTGGATTTGAGCAATGTTGCTGATACCGAAGAAGACcgcg                  >  2:3520433/1‑150 (MQ=255)
                                                                                                                                       aCTGCCCGGCAGCAGAAAGCCTTTCTTATCAACAAGCTTTCTTGCGTTATCTGGAAATTGACCCGCTCTCTGCCGACAAAACGCAACTGCGGGAAGTCGCAGCGAAACTGGATTTGAGCAATGTTGCTGATACCGAAGAAGACCGCGaca               >  1:3821088/1‑150 (MQ=255)
                                                                                                                                              ggCAGCAGAAAGCCTTTCTTATCAACAAGCTTTCTTGCGTTATCTGGAAATTGACCCGCTCTCTGCCGACAAAACGCAACTGCGGGAAGTCGCAGCGAAACTGGATTTGAGCAATGTTGCTGATACCGAAGAAGACCGCGACACgctgct        <  1:1802764/150‑1 (MQ=255)
                                                                                                                                                    agAAAGCCTTTCTTATCAACAAGCTTTCTTGCGTTATCTGGAAATTGACCCGCTCTCTGCCGACAAAACGCAACTGCGGGAAGTCGCAGCGAAACTGGATTTGAGCAATGTTGCTGATACCGAAGAAGACCGCGACACGCTGCTACAAtt  >  1:1618068/1‑150 (MQ=255)
                                                                                                                                                    |                                                                                                                                                     
TGTGCCGCAGCTTCCGTAATGAAGAGATGGGGCGTTATCACAACCCTGAGTTCACTATGCTGGAGTGGTATCGACCGCACTATGATATGTACCGGTTGATGAACGAGGTGGACGATCTCTTACAACAGGTGCTGGACTGCCCGGCAGCAGAAAGCCTTTCTTATCAACAAGCTTTCTTGCGTTATCTGGAAATTGACCCGCTCTCTGCCGACAAAACGCAACTGCGGGAAGTCGCAGCGAAACTGGATTTGAGCAATGTTGCTGATACCGAAGAAGACCGCGACACGCTGCTACAATT  >  CP009273/4372743‑4373040

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑