Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 404,324 C→A 15.1% pseudogene (260/342 nt) ykiA → pseudogene

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273404,3240CA15.1% 822.2 / 100.8 272pseudogene (260/342 nt)ykiApseudogene
Reads supporting (aligned to +/- strand):  ref base C (118/113);  new base A (20/21);  total (138/134)
Fisher's exact test for biased strand distribution p-value = 8.66e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.78e-01

Only 100 of 277 total aligned reads displayed.

ACATACCCCGGCGTTGTTGTTAACGACATTGACTGAGCCTCAGGATCGGTCGCTGGCTATTAATAATCCACAGCTGGCTGCCGATGTGAAAACGGCGTGGTTAAAAGAGGATCCATCATTACTCTTATTTGTCGAACAACCCGATCTTTCGCTGTTACGTGATTTAGTGAAAACCGGGGCAACGCGGAAAATTCGCAGTGAAGCGCGTCACCGGCTTGAGGAAAAACAATGACACAACGTCCATGGAGCAAGTTACAGCGTAAAACGCATAATATTGCTGCGCTAAAAATTATTGCTCG  >  CP009273/404175‑404473
                                                                                                                                                     |                                                                                                                                                     
acaTACCCCGGCGTTGTTGTTAACGACCTTGACTGAGCCTCAGGATCGGTCCCGGGCGATTAATAATCCACAGCGGGCTGCCGAGGTGAAAACGGCGGGGGTAAAAGAGGAGCCCACCATACACTTATTTGTCGAACAACCCCCTCTTTc                                                                                                                                                       >  2:1622595/1‑150 (MQ=255)
acaTACCCCGGCGTTGTTGTTAAAGACATTGACTGTGCCTCAGGATCGGTCGCTGGCTATTAATAATCCACAGCTGGCTGCCGATGTGAAAACGGCGTGGTTAAAAGAGGATCCATCATTACTCTTATTTGTCGAACAACCCGATCTTTc                                                                                                                                                       >  2:1873970/1‑150 (MQ=255)
    aCCCCGGCGTTGTTGTTAACGACATTGACTGAGCCTCAGGATCGGTCGCTGGCTATTAATAATCCACAGCTGGCTGCCGATGTGAAAACGGCGTGGTTAAAAGAGGATCCATCATTACTCTTATTTGTCGAACAACCCGATCTTTCGCTg                                                                                                                                                   <  1:4125028/150‑1 (MQ=255)
      cccGGCGTTGTTGTTAACGACATTGACTGAGCCTCAGGATCGGTCGCTGGCTATTAATAATCCACAGCTGGCTGCCGATGTGAAAACGGCGTGGTTAAAAGAGGATCCATCATTACTCTTATTTGTCGAACAACCCGATCTTTCGCTGtt                                                                                                                                                 >  2:4289282/1‑150 (MQ=255)
         ggCGTTGTTGTTAACGACATTGACTGAGCCTCAGGATCGGTCGCTGGCTATTAATAATCCACAGCTGGCTGCCGATGTGAAAACGGCGTGGTTAAAAGAGGATCCATCATTACTCTTATTTGTCGAACAACCCGATCTTTCGCTGTTACg                                                                                                                                              >  1:1328677/1‑150 (MQ=255)
         ggCGTTGTTGTTAACGACATTGACTGAGCATCAGGATCGGTCGCTGGCTATTAATATTCCACAGCTGGCTGCCGATGTGAAAACGGCGTGGTTAAAAGAGGATCCATCATTACTCTTATTTGTCGAACAACCCGATCTTTCGCTGTTACg                                                                                                                                              <  1:1910481/150‑1 (MQ=255)
           cGTTGTTGTTAACGACATTGACTGAGCCTCAGGATCGGTCGCTGGCTATTAATAATCCACAGCTGGCTGCCGATGTGAAAACGGCGTGGTTAAAAGAGGATCCATCATTACTCTTATTTGTCGAACAACCCGATCTTTCGCTGTTACGTg                                                                                                                                            <  1:3366788/150‑1 (MQ=255)
               gttgttAACGACATTGACTGAGCCTCAGGATCGGTCGCTGGCTATTAATAATCCACAGCTGGCTGCCGATGTGAAAACGGCGTGGTTAAAAGAGGATCCATCATTACTCTTATTTGTCGAACAACCCGATCTTTCGCTGTTACGTGAttt                                                                                                                                        >  2:100528/1‑150 (MQ=255)
                ttgttAACGACATTGACTGAGCCTCAGGATCGGTCGCTGGCTATTAATAATCCACAGCTGGCTGCCGATGTGAAAACGGCGTGGTTAAAAGAGGATCCATCATTACTCTTATTTGTCGAACAACCCGATCTTTCGCTGTTACGTGATTTa                                                                                                                                       <  1:1048721/150‑1 (MQ=255)
                    tAACGACATTGACTGAGCCTCAGGATCGGTCGCTGGCTATTAATAATCCACAGCTGGCTGCCGATGTGAAAACGGCGTGGTTAAAAGAGGATCCATCATTACTCTTATTTGTCGAACAACCCGATCTTTCGCTGTTACGTGATTTAGTGa                                                                                                                                   <  2:2495885/150‑1 (MQ=255)
                    tAACGACATTGACTGAGCCTCAGGATCGGTCGCTGGCTATTAATAATCCACAGCTGGCTGCCGATGTGAAAAAGGCGTGGTTAAAAGAGGATCCATCAATAATCTTATTTGTCGAACAACCCGATCTTTCGCTGTTACGTGATTTAGTGa                                                                                                                                   >  1:2661288/1‑150 (MQ=255)
                     aaCGACATTGACTGAGCCTCAGGATCGGTCGCTGGCTATTAATAATCCACAGCTGGCTGCCGATGTAAAAACGGCGTGGTTAAAAGAGGATCCATCATTACTCTTATTTGTCGAACAACCCGATCTTTCGCTGTTACGTGATTTAGTGaa                                                                                                                                  >  1:2573302/1‑150 (MQ=255)
                       cGACATTGACTGAGCCTCAGGATCGGTCGCTGGCTATTAATAATCCACAGCTGGCTGCCGATGTGAAAACGGCGTGGTTAAAAGAGGATCCATCATTACTCTTATTTGTCGAACAACCCGATCTTTAGCTGTTACGTGATTTAGTGaaaa                                                                                                                                <  2:1197625/150‑1 (MQ=255)
                            ttGACTGAGCCTCAGGATCGGTCGCTGGCTATTAATAATCCACAGCTGGCTGCCGATGTGAAAACGGCGTGGTTAAAAGAGGATCCATCATTACTCTTATTTGTCGAACAACCCGATCTTTAGCTGTTACGTGATTTAGTGAAAACCggg                                                                                                                           <  1:2553488/150‑1 (MQ=255)
                               aCTGAGCCTCAGGATCGGTCGCTGGCTATTAATAATCCACAGCTGGCTGCCGATGTGAAAACGGCGTGGTTAAAAGAGGATCCATCATTACTCTTATTTGTCGAACAACCCGATCTTTCGCTGTTACGTGATTTAGTGAAAACCGGGGCa                                                                                                                        <  1:3338270/150‑1 (MQ=255)
                                 tGAGCCTCAGGATCGGTCGCTGGCTATTAATAATCCACAGCTGGCTGCCGATGTGAAAACGGCGTGGTTAAAAGAGGATCCATCATTACTCTTATTTGTCGAACAACCCGATCTTTAGCTGTTACGTGATTTAGTGAAAACCGGGGCAAc                                                                                                                      <  2:1307735/150‑1 (MQ=255)
                                     ccTCAGGATCGGTCGCTGGCTATTAATAATCCACAGCTGGCTGCCGATGTGAAAACGGCGTGGTTAAAAGAGGATCCATCATTACTCTTATTTGTCGAACAACCCGATCTTTAGCTGTTACGTGATTTAGTGAAAACCGGGGCAACGCgg                                                                                                                  <  2:578342/150‑1 (MQ=255)
                                       tCAGGATCGGTCGCTGGCTATTAATAATCCACAGCTGGCTGCCGATGTGAAAACGGCGTGGTTAAAAGAGGATCCATCATTACTCTTATTTGTCGAACAACCCGATCTTTCGCTGTTACGTGATTTAGTGAAAACCGGGGCAACGCGGaa                                                                                                                <  2:1435178/150‑1 (MQ=255)
                                         aGGATCGGTCGCTGGCTATTAATAATCCACAGCTGGCTGCCGATGTGAAAACGGCGTGGTTAAAAGAGGATCCATCATTACTCTTATTTGTCGAACAACCCGATCTTTCGCTGTTACGTGATTTAGTGAAAACCGGGGCAACGCGGaaaa                                                                                                              >  2:2602177/1‑150 (MQ=255)
                                          ggATCGGTCGCTGGCTATTAATAATCCACAGCTGGCTGCCGATGTGAAAACGGCGTGGTTAAAAGAGGATCCATCATTACTCTTATTTGTCGAACAACCCGATCTTTCGCTGTTACGTGATTTAGTGAAAACCGGGGCAACGCGGAAAAt                                                                                                             >  1:2122955/1‑150 (MQ=255)
                                          ggATCGGTCGCTGGCTATTAATAATCCACAGCTGGCTGCCGATGTGAAAACGGCGTGGTTAAAAGAGGATCCATCATTACTCTTATTTGTCGAACAACCCGATCTTTCGCTGTTACGTGATTTAGTGAAAACCGGGGCAACGCGGAAAAt                                                                                                             <  1:4415890/150‑1 (MQ=255)
                                           gATCGGTCGCTGGCTATTAATAATCCACAGCTGGCTGCCGATGTGAAAACGGCGTGGTTAAAAGAGGATCCATCATTACTCTTATTTGTCGAACAACCCGATCTTTCGCTGTTACGTGATTTAGTGAAAACCGGGGCAACGCGGAAAAtt                                                                                                            <  2:2065028/150‑1 (MQ=255)
                                             tCGGTCGCTGGCTATTAATAATCCACAGCTGGCTGCCGATGTGAAAACGGCGTGGTTAAAAGAGGATCCGTCATTACTCTTATTTGTCGAACAACCCGATCTTTCGCTGTTACGTGATTTAGTGAAAACCGGGGCAACGCGGAAAATTCg                                                                                                          >  2:359037/1‑150 (MQ=255)
                                             tCGGTCGCTGGCTATTAATAATCCACAGCTGGCTGCCGATGTGAAAACGGCGTGGTTAAAAGAGGATCCATCATTACTCTTATTTGTCGAACAACCCGATCTTTCGCTGTTACGTGATTTAGTGAAAACCGGGGCAACGCGGAAAATTCg                                                                                                          >  2:2142054/1‑150 (MQ=255)
                                              cGGTCGCTGGCTATTAATAATCCACAGCTGGCTGCCGATGTGAAAACGGCGTGGTTAAAAGAGGATCCATCATTACTCTTATTTGTCGAACAACCCGATCTTTCGCTGTTACGTGATTTAGTGAAAACCGGGGCAACGCGGAAAATTCGc                                                                                                         >  1:2875769/1‑150 (MQ=255)
                                                        cTATTAATAATCCACAGCTGGCTGCCGATGTGAAAACGGCGTGGTTAAAAGAGGATCCATCATTACTCTTATTTGTCGAACAACCCGATCTTTCGCTGTTACGTGATTTAGTGAAAACCGGGGCAACGCGGAAAATTCGCAGTGAAgcgc                                                                                               >  2:2231109/1‑150 (MQ=255)
                                                        cTATTAATAATCCACAGCTGGCTGCCGATGTGAAAACGGCGTGGTTAAAAGAGGATCCATCATTACTCTTATTTGTCGAACAACCCGATCTTTCGCTGTTACGTGATTTAGTGAAAACCGGGGCAACGCGGAAAATTCGCAGTGAAgcgc                                                                                               >  2:466692/1‑150 (MQ=255)
                                                         tATTAATAATCCACAGCTGGCTGCCGATGTGAAAACGGCGTGGTTAAAAGAGGATCCATCATTACTCTTATTTGTCGAACAACCCGATCTTTCGCTGTTACGTGATTTAGTGAAAACCGGGGCAACGCGGAAAATTCGCAGTGAAGcgcg                                                                                              <  1:1577763/150‑1 (MQ=255)
                                                           ttAATAATCCACAGCTGGCTGCCGATGTGAAAACGGCGTGGTTAAAAGAGGATCCATCATTACTCTTATTTGTCGAACAACCCGATCTTTAGCTGTTACGTGATTTAGTGAAAACCGGGGCAACGCGGAAAATTCGCAGTGAAGCGCGTc                                                                                            <  2:1556647/150‑1 (MQ=255)
                                                              ataatCCACAGCTGGCTGCCGATGTGAAAACGGCGTGGTTAAAAGAGGATCCATCATTACTCTTATTTGTCGAACAACCCGATCTTTCGCTGTTACGTGATTTAGTGAAAACCGGGGCAACGCGGAAAATTCGCAGTGAAGCGCGTCAcc                                                                                         >  2:3909237/1‑150 (MQ=255)
                                                              ataatCCACAGCTGGCTGCCGATGTGAAAACGGCGTGGTTAAAAGAGGATCCATCATTACTCTTATTTGTCGAACAACCCGATCTTTCGCTGTTACGTGATTTAGTGAAAACCGGGGCAACGCGGAAAATTCGCAGTGAAGCGCGTCAcc                                                                                         >  2:255959/1‑150 (MQ=255)
                                                                aatCCACAGCTGGCTGCCGATGTGAAAACGGCGTGGTTAAAAGAGGATCCATCATTACTCTTATTTGTCGAACAACCCGATCTTTCGCTGTTACGTGATTTAGTGAAAACCGGGGCAACGCGGAAAATTCGCAGTGAAGCGCGTCACCgg                                                                                       >  2:764862/1‑150 (MQ=255)
                                                                   ccACAGCTGGCTGCCGATGTGAAAACGGCGTGGTTAAAAGAGGATCCATCATTACTCTTATTTGTCGAACAACCCGATCTTTCGCTGTTACGTGATTTAGTGAAAACCGGGGCAACGCGGAAAATTCGCAGTGAAGCGCGTCACCGGCtt                                                                                    <  2:2271459/150‑1 (MQ=255)
                                                                     acaGCTGGCTGCCGATGTGAAAACGGCGTGGTTAAAAGAGGATCCATCATTACTCTTATTTGTCGAACAACCCGATCTTTAGCTGTTACGTGATTTAGTGAAAACCGGGGCAACGCGGAAAATTCGCAGTGAAGCGCGTCACCGGCTTGa                                                                                  >  1:3952529/1‑150 (MQ=255)
                                                                          tggctgTCGATGTGAAAACGGCGTGGTTAAAAGAGGATCCATCATTACTCTTATTTGTCGAACAACCCGATCTTTCGCTGTTACGTGATTTAGTGAAAACCGGGGCAACGCGGAAAATTCGCAGTGAAGCGCGTCACCGGCTTGAGGaaa                                                                             >  2:1797155/1‑150 (MQ=255)
                                                                          tggctgCCGATGTGAAAACGGCGTGGTTAAAAGAGGATCCATCATTACTCTTATTTGTCGAACAACCCGATCTTTCGCTGTTACGTGATTTAGTGAAAACCGGGGCAACGCGGAAAATTCGCAGTGAAGCGCGTCACCGGCTTGAGGaaa                                                                             >  2:2192338/1‑150 (MQ=255)
                                                                          tggctgCCGATGTGAAAACGGCGTGGTTAAAAGAGGATCCATCATTACTCTTATTTGTCGAACAACCCGATCTTTCGCTGTTACGTGATTTAGTGAAAACCGGGGCAACGCGGAAAATTCGCAGTGAAGCGCGTCACCGGCTTGAGGaaa                                                                             >  2:3532764/1‑150 (MQ=255)
                                                                           ggctgCCGATGTGAAAACGGCGTGGTTAAAAGAGGATCCATCATTACTCTTATTTGTCGAACAACCCGATCTTTCGCTGTTACGTGATTTAGTGAAAACCGGGGCAACGCGGAAAATTCGCAGTGAAGCGCGTCACCGGCTTGAGGaaaa                                                                            <  1:3900726/150‑1 (MQ=255)
                                                                           ggctgCCGATGTGAAAACGGCGTGGTTAAAAGAGGATCCATCATTACTCTTATTTGTCGAACAACCCGATCTTTCGCTGTTACGTGATTTAGTGAAAACCGGGGCAACGCGGAAAATTCGCAGTGAAGCGCGTCACCGGCTTGAGGaaaa                                                                            <  2:2960248/150‑1 (MQ=255)
                                                                                 cGATGTGAAAACGGCGTGGTTAAAAGAGGATCCATCATTACTCTTATTTGTCGAACAACCCGATCTTTCGCTGTTACGTGATTTAGTGAAAACCGGGGCAACGCGGAAAATTCGCAGTGAAGCGCGTCACCGGCTTGAGGAAAAACAATg                                                                      >  1:510635/1‑150 (MQ=255)
                                                                                     gtgAAAACGGCGTGGTTAAAAGAGGATCCATCATTACTCTTATTTGTCGAACAACCCGATCTTTCGCTGTTACGTGATTTAGTGAAAACCGGGGCAACGCGGAAAATTCGCAGTGAAGCGCGTCACCGGCTTGAGGAAAAACAATGacac                                                                  <  2:4138355/150‑1 (MQ=255)
                                                                                      tgAAAACGGCGTGGTTAAAAGAGGATCCATCATTACTCTTATTTGTCGAACAACCCGATCTTTCGCTGTTACGTGATTTAGTGAAAACCGGGGCAACGCGGAAAATTCGCAGTGAAGCGCGTCACCGGCTTGAGGAAAAACAATGAcaca                                                                 >  1:1665822/1‑150 (MQ=255)
                                                                                      tgAAAACGGCGTGGTTAAAAGAGGATCCATCATTACTCTTATTTGTCGAACAACCCGATCTTTAGCTGTTACGTGATTTAGTGAAAACCGGGGCAACGCGGAAAATTCGCAGTGAAGCGCGTCACCGGCTTGAGGAAAAACAATGAcaca                                                                 <  2:3762214/150‑1 (MQ=255)
                                                                                        aaaaCGGCGTGGTTAAAAGAGGATCCATCATTACTCTTATTTGTCGAACAACCCGATCTTTCGCTGTTACGTGATTTAGTGAAAACCGGGGCAACGCGGAAAATTCGCAGTGAAGCGCGTCACCGGCTTGAGGAAAAACAATGACACAAc                                                               >  2:379710/1‑150 (MQ=255)
                                                                                              gCGTGGTTAAAAGAGGATCCATCATTACTCTTATTTGTCGAACAACCCGATCTTTCGCTGTTACGTGATTTAGTGAAAACCGGGGCAACGCGGAAAATTCGCAGTGAAGCGCGTCACCGGCTTGAGGAAAAACAATGACACAACGTCCAt                                                         <  2:2645436/150‑1 (MQ=255)
                                                                                                  ggTTAAAAGAGGATCCATCATTACTCTTATTTGTCGAACAACCCGATCTTTCGCTGTTACGTGATTTAGTGAAAACCGGGGCAACGCGGAAAATTCGCAGTGAAGCGCGTCACCGGCTTGAGGAAAAACAATGACACAACGTCCATGGAg                                                     >  2:2975278/1‑150 (MQ=255)
                                                                                                  ggTTAAAAGAGGATCCATCATTACTCTTATTTGTCGAACAACCCGATCTTTCGCTGTTACGTGATTTAGTGAAAACCGGGGCAACGCGGAAAATTCGCAGTGAAGCGCGTCACCGGCTTGAGGAAAAACAATGACACAACGTCCATGGAg                                                     <  2:2814956/150‑1 (MQ=255)
                                                                                                        aaGAGGATCCATCATTACTCTTATTTGTCGAACAACCCGATCTTTCGCTGTTACGTGATTTAGTGAAAACCGGGGCAACGCGGAAAATTCGCAGTGAAGCGCGTCACCGGCTTGAGGAAAAACAATGACACAACGTCCATGGAGCAAGtt                                               >  2:2047319/1‑150 (MQ=255)
                                                                                                           agGATCCATCATTACTCTTATTTGTCGAACAACCCGATCTTTCGCTGTTACGTGATTTAGTGAAAACCGGGGCAACGCGGAAAATTCGCAGTGAAGCGCGTCACCGGCTTGAGGAAAAACAATGACACAACGTCCATGGAGCAAGTTACa                                            >  1:3524722/1‑150 (MQ=255)
                                                                                                           agGATCCATCATTACTCTTATTTGTCGAACAACCCGATCTTTAGCTGTTACGTGATTTAGTGAAAACCGGGGCAACGCGGAAAATTCGCAGTGAAGCGCGTCACCGGCTTGAGGAAAAACAATGACACAACGTCCATGGAGCACGTTACa                                            >  1:4221978/1‑150 (MQ=255)
                                                                                                             gATCCATCATTACTCTTATTTGTCGAACAACCCGATCTTTCGCTGTTACGTGATTTAGTGAAAACCGGGGCAACGCGGAAAATTCGCAGTGAAGCGCGTCACCGGCTTGAGGAAAAACAATGACACAACGTCCATGGAGCAAGTTACAGc                                          >  2:2169332/1‑150 (MQ=255)
                                                                                                              aTCCATCATTACTCTTATTTGTCGAACAACCCGATCTTTCGCTGTTACGTGATTTAGTGAAAACCGGGGCAACGCGGAAAATTCGCAGTGAATCGCGTCACCGGCTTGAGGAAAAACAATGACACAACGTCCATGGAGCAAGTTACAGCg                                         >  2:1672428/1‑150 (MQ=255)
                                                                                                              aTCCATCATTACTCTTATTTGTCGAACAACCCGATCTTTAGCTGTTACGTGATTTAGTGAAAACCGGGGCAACGCGGAAAATTCGCAGTGAAGCGCGTCACCGGCTTGAGGAAAAACAATGACACAACGTCCATGGAGCAAGTTACAGCg                                         >  2:31017/1‑150 (MQ=255)
                                                                                                               tCCATCATTACTCTTATTTGTCGAACAACCCGATCTTTCGCTGTTACGTGATTTAGTGAAAACCGGGGCAACGCGGAAAATTCGCAGTGAAGCGCGTCACCGGCTTGAGGAAAAACAATGACACAACGTCCATGGAGCAAGTTACAGCGt                                        <  1:1785870/150‑1 (MQ=255)
                                                                                                                ccATCATTACTCTTATTTGTCGAACAACCCGATCTTTCGCTGTTACGTGATTTAGTGAAAACCGGGGCAACGCGGAAAATTCGCAGTGAAGCGCGTCACCGGCTTGAGGAAAAACAATGACACAACGTCCATGGAGCAAGTTACAGCGTa                                       >  1:4027333/1‑150 (MQ=255)
                                                                                                                 catcatTACTCTTATTTGTCGAACAACCCGATCTTTAGCTGTTACGTGATTTAGTGAAAACCGGGGCAACGCGGAAAATTCGCAGTGAAGCGCGTCACCGGCTTGAGGAAAAACAATGACACAACGTCCATGGAGCAAGTTACAGCGTaa                                      >  2:329580/1‑150 (MQ=255)
                                                                                                                  atcatTACTCTTATTTGTCGAACAACCCGATCTTTCGCTGTTACGTGATTTAGTTAAAACCGGGGCAACGCGGAAAATTCGCAGTGAAGCGCGTCACCGGATTGAGGAAAAACAATGACACAACGTCCATGGAGCAAGTTACAGCGTaaa                                     <  1:3786191/150‑1 (MQ=255)
                                                                                                                  atcatTACTCTTATTTGTCGAACAACCCGATCTTTCGCTGTTACGTGATTTAGTGAAAACCGGGGCAACGCGGAAAATTCGCAGTGAAGCGCGTCACCGGCTTGAGGAAAAACAATGACACAACGTCCATGGAGCAAGTTACAGCGTaaa                                     >  1:635313/1‑150 (MQ=255)
                                                                                                                  atcatTACTCTTATTTGTCGAACAACCCGATCTTTCGCTGTTACGTGATTTAGTGAAAACCGGGGCAACGCGGAAAATTCGCAGTGAAGCGCGTCACCGGCTTGAGGAAAAACAATGACACAACGTCCATGGAGCAAGTTACAGCGTaaa                                     >  1:2410121/1‑150 (MQ=255)
                                                                                                                   tcatTACTCTTATTTGTCGAACAACCCGATCTTTCGCTGTTACGTGATTTAGTGAAAACCGGGGCAACGCGGAAAATTCGCAGTGAAGCGCGTCACCGGCTTGAGGAAAAACAATGACACAACGTCCATGGAGCAAGTTACAGCGTaaaa                                    >  2:3824878/1‑150 (MQ=255)
                                                                                                                   tcatTACTCTTATTTGTCGAACAACCCGATCTTTCGCTGTTACGTGATTTAGTGAAAACCGGGGCAACGCGGAAAATTCGCAGTGAAGCGCGTCACCGGCTTGAGGAAAAACAATGACACAACGTCCATGGAGCAAGTTACAGCGTaaaa                                    <  1:2962462/150‑1 (MQ=255)
                                                                                                                   tcatTACTCTTATTTGTCGAACAACCCGATCTTTCGCTGTTACGTGATTTAGTGAAAACCGGGGCAACGCGGAAAATTCGCAGTGAAGCGCGTCACCGGCTTGAGGAAAAACAATGACACAACGTCCATGGAGCAAGTTACAGCGTaaaa                                    <  1:2805669/150‑1 (MQ=255)
                                                                                                                    catTACTCTTATTTGTCGAACAACCCGATCTTTCGCTGTTACGTGATTTAGTGAAAACCGGGGCAACGCGGAAAATTCGCAGTGAAGCGCGTCACCGGCTTGAGGAAAAACAATGACACAACGTCCATGGAGCAAGTTACAGCGTAAAAc                                   <  2:27618/150‑1 (MQ=255)
                                                                                                                    catTACTCTTATTTGTCGAACAACCCGATCTTTAGCTGTTACGTGATTTAGTGAAAACCGGGGCAACGCGGAAAATTCGCAGTGAAGCGCGTCACCGGCTTGAGGAAAAACAATGACACAACGTCCATGGAGCAAGTTACAGCGTAAAAc                                   <  2:325733/150‑1 (MQ=255)
                                                                                                                    aatTACTCGTATTTGTCGAACAACCCGATCTTTCGCTGTTACGTGATTTAGTGAAAACCGGGGCAACGCGGAAAATTCGCAGTGAAGCGCGTCACCGGCTTGAGGAAAAACAATGACACAACGTCCATGGAGCAAGTTACAGCGTAAAAc                                   <  2:3036140/149‑1 (MQ=255)
                                                                                                                     atTACTCTTATTTGTCGAACAACCCGATCTTTCGCTGTTACGTGATTTAGTGAAAACCGGGGCAACGCGGAAAATTCGCAGTGAAGCGCGTCACCGGCTTGAGGAAAAACAATGACACAACGTCCATGGAGCAAGTTACAGCGTAAAACg                                  >  1:3664728/1‑150 (MQ=255)
                                                                                                                     atTACTCTTATTTGTCGAACAACCCGATCTTTCGCTGTTACGTGATTTAGTGAAAACCGGGGCAACGCGGAAAATTCGCAGTGAAGCGCGTCACCGGCTTGAGGAAAAACAATGACACAACGTCCATGGAGCAAGTTACAGCGTAAAACg                                  <  1:4300223/150‑1 (MQ=255)
                                                                                                                      ttACTCTTATTTGTCGAACAACCCGATCTTTCGCTGTTACGTGATTTAGTGAAAACCGGGGCAACGCGGAAAATTCGCAGTGAAGCGCGTCACCGGCTTGAGGAAAAACAATGACACAACGTCCATGGAGCAAGTTACAGCGTAAAACGc                                 >  1:791164/1‑150 (MQ=255)
                                                                                                                        aCTCTTATTTGTCGAACAACCCGATCTTTCGCTGTTACGTGATTTAGTGAAAACCGGGGCAACGCGGAAAATTCGCAGTGAAGCGCGTCACCGGCTTGAGGAAAAACAATGACACAACGTCCATGGAGCAAGTTACAGCGTAAAACGCat                               >  2:4524008/1‑150 (MQ=255)
                                                                                                                            ttATTTGTCGAACAACCCGATCTTTCGCTGTTACGTGATTTAGTGAAAACCGGGGCAACGCGGAAAATTCGCAGTGAAGCGCGTCACCGGCTTGAGGAAAAACAATGACACAACGTCCATGGAGCAAGTTACAGCGTAAAACGCataata                           >  1:896845/1‑150 (MQ=255)
                                                                                                                            ttATTTGTCGAACAACCCGATCTTTCGCTGTTACGTGATTTAGTGAAAACCGGGGCAACGCGGAAAATTCGCAGTGAAGCGCGTCACCGGCTTGAGGAAAAACAATGACACAACGTCCATGGAGCAAGTTACAGCGTAAAACGCataata                           >  1:896810/1‑150 (MQ=255)
                                                                                                                             tATTTGTCGAACAACCCGATCTTTCGCTGTTACGTGATTTAGTGAAAACCGGGGCAACGCGGAAAATTCGCAGTGAAGCGCGTCACCGGCTTGAGGAAAAACAATGACACAACGTCCATGGAGCAAGTTACAGCGTAAAACGCATAatat                          >  2:204490/1‑150 (MQ=255)
                                                                                                                             tATTTGTCGAACAACCCGATCTTTCGCTGTTACGTGATTTAGTGAAAACCGGGGCAACGCGGAAAATTCGCAGTGAAGCGCGTCACCGGCTTGAGGAAAAACAATGACACAACGTCCATGGAGCAAGTTACAGCGTAAAACGCATAatat                          <  1:100528/150‑1 (MQ=255)
                                                                                                                             tATTTGTCGAACAACCCGATCTTTCGCTGTTACGTGATTTAGTGAAAACCGGGGCAACGCGGAAAATTCGCAGTGAAGCGCGTCACCGGCTTGAGGAAAAACAATGACACAACGTCCATGGAGCAAGTTACAGCGTAAAACGCATAatat                          <  2:61014/150‑1 (MQ=255)
                                                                                                                                 tGTCGAACAACCCGATCTTTCGCTGTTACGTGATTTAGTGAAAACCGGGGCAACGCGGAAAATTCGCAGTGAAGCGCGTCACCGGCTTGAGGAAAAACAATGACACAACGTCCATGGAGCAAGTTACAGCGTAAAACGCATAATATtgct                      <  1:604350/150‑1 (MQ=255)
                                                                                                                                   tCGAACAACCCGATCTTTCGCTGTTACGTGATTTAGTGAAAACCGGGGCAACGCGGAAAATTCGCAGTGAAGCGCGTCACCGGCTTGAGGAAAAACAATGACACAACGTCCATGGAGCAAGTTACAGCGTAAAACGCATAATATtgctgc                    >  2:4029711/1‑150 (MQ=255)
                                                                                                                                   tCGAACAACCCGATCTTTCGCTGTTACGTGATTTAGTGAAAACCGGGGCAACGCGGAAAATTCGCAGTGAAGCGCGTCACCGGCTTGAGGAAAAACAATGACACAACGTCCATGGAGCAAGTTACAGCGTAAAACGCATAATATtgctgc                    <  2:2735477/150‑1 (MQ=255)
                                                                                                                                   tCGAACAACCCGATCTTTCGCTGTTACGTGATTTAGTGAAAACCGGGGCAACGCGGAAAATTCGCAGTGAAGCGCGTCACCGGCTTGAGGAAAAACAATGACACAACGTCCATGGAGCAAGTTACAGCGTAAAACGCATAATATtgctgc                    <  1:4332476/150‑1 (MQ=255)
                                                                                                                                    cGAACAACCCGATCTTTCGCTGTTACGTGATTTAGTGAAAACCGGGGCAACGCGGAAAATTCGCAGTGAAGCGCGTCACCGGCTTGAGGAAAAACAATGACACAACGTCCATGGAGCAAGTTACAGCGTAAAACGCATAATATTGCTgcg                   >  2:1149774/1‑150 (MQ=255)
                                                                                                                                    cGAACAACCCGATCTTTCGCTGTTACGTGATTTAGTGAAAACCGGGGCAACGCGGAAAATTCGCAGTGAAGCGCGTCACCGGCTTGAGGAAAAACAATGACACAACGTCCATGGAGCAAGTTACAGCGTAAAACGCATAATATTGCTgcg                   >  1:4109994/1‑150 (MQ=255)
                                                                                                                                    cGAACAACCCGATCTTTAGCTGTTACGTGATTTAGTGAAAACCGGGGCAACGCGGAAAATTCGCAGTGAAGCGCGTCACCGGCTTGAGGAAAAACAATGACACAACGTCCATGGAGCAAGTTACAGCGTAAAACGCATAATATTGCTgcg                   <  1:1936657/150‑1 (MQ=255)
                                                                                                                                      aacaacCCGATCTTTCGCTGTTACGTGATTTAGTGAAAACCGGGGCAACGCGGAAAATTCGCTGTGAAGCGCGTCACCGGATTGAGGAAAAACAATGACACAACGTCCATGGAGCAAGTTACAGCGTAAAACGCATAATATTGCTGCGCt                 <  1:1525815/150‑1 (MQ=255)
                                                                                                                                      aacaacCCGATCTTTCGCTGTTACGTGATTTAGTGAAAACCGGGGCAACGCGGAAAATTCGCAGTGAAGCGCGTCACCGGCTTGAGGAAAAACAATGACACAACGTCCATGGAGCAAGTTACAGCGTAAAACGCATAATATTGCTGCGCt                 >  2:47316/1‑150 (MQ=255)
                                                                                                                                       acaacCCGATCTTTCGCTGTTACGTGATTTAGTGAAAACCGGGGCAACGCGGAAAATTCGCAGTGAAGCGCGTCACCGGCTTGAGGAAAAACAATGACACAACGTCCATGGAGCAAGTTACAGCGTAAAACGCATAATATTGCTGCGCTa                <  2:3710267/150‑1 (MQ=255)
                                                                                                                                         aacCCGATCTTTCGCTGTTACGTGATTTAGTGAAAACCGGGGCAACGCGGAAAATTCGCAGTGAAGCGCGTCACCGGCTTGAGGAAAAACAATGACACAACGTCCATGGAGCAAGTTACAGCGTAAAACGCATAATATTGCTGCGCTaaa              <  2:910492/150‑1 (MQ=255)
                                                                                                                                         aacCCGATCTTTCGCTGTTACGTGATTTAGTGAAAACCGGGGCAACGCGGAAAATTCGCAGTGAAGCGCGTCACCGGCTTGAGGAAAAACAATGACACAACGTCCATGGAGCAAGTTACAGCGTAAAACGCATAATATTGCTGCGCTaaa              <  2:2630766/150‑1 (MQ=255)
                                                                                                                                         aacCCGATCTTTCGCGGGTACGTGATTTAGTGAAAACCGGGGCAACGCGGAAAATTCGCAGTGAAGCGCGTCACCGGCTTGAGGAAAAACAATGACACAACGTCCATGGAGCAAGTTACAGCGTAAAACGCATAATATTGCTGCGCTaaa              <  2:817231/150‑1 (MQ=255)
                                                                                                                                          aaccGATCTTTCGCTGTTACGTGATTTAGTGAAAACCGGGGCAACGCGGAAAATTCGCAGTGAAGCGCGTCACCGGCTTGAGGAAAAACAATGACACAACGTCCATGGAGCAAGTTACAGCGTAAAACGCATAATATTGCTGCGCTaaaa             <  2:659916/148‑1 (MQ=255)
                                                                                                                                           cccGATCTTTCGCTGTTACGTGATTTAGTGAAAACCGGGGCAACGCGGAAAATTCGCAGTGAAGCGCGTCACCGGCTTGAGGAAAAACAATGACACAACGTCCATGGAGCAAGTTACAGCGTAAAACGCATAATATTGCTGCGCTaaaaa            >  2:3176805/1‑150 (MQ=255)
                                                                                                                                              gATCTTTCGCTGTTACGTGATTTAGTGAAAACCGGGGCAACGCGGAAAATTCGCAGTGAAGCGCGTCACCGGCTTGAGGAAAAACAATGACACAACGTCCATGGAGCAAGTTACAGCGTAAAACGCATAATATTGCTGCGCTAAAAatta         >  2:20150/1‑150 (MQ=255)
                                                                                                                                              gATCTTTCGCTGTTACGTGATTTAGTGAAAACCGGGGCAACGCGGAAAATTCGCAGTGAAGCGCGTCACCGGCTTGAGGAAAAACAATGACACAACGTCCATGGAGCAAGTTACAGCGTAAAACGCATAATATTGCTGCGCTAAAAatta         <  2:2122955/150‑1 (MQ=255)
                                                                                                                                              gATCTTTCGCTGTTACGTGATTTAGTGAAAACCGGGGCAACGCGGAAAATTCGCAGTGAAGCGCGTCACCGGCTTGAGGAAAAACAATGACACAACGTCCATGGAGCAAGTTACAGCGTAAAACGCATAATATTGCTGCGCTAAAAatta         <  2:4400352/150‑1 (MQ=255)
                                                                                                                                              gATCTTTCGCTGTTACGTGATTTAGTGAAAACCGGGGCAACGCGGAAAATTCGCAGTGAAGCGCGTCACCGGCTTGAGGAAAAACAATGACACAACGTCCATGGAGCAAGTTACAGCGTAAAACGCATAATATTGCTGCGCTAAAAatta         >  2:1076812/1‑150 (MQ=255)
                                                                                                                                               aTCTTTAGCTGTTACGTGATTTAGTGAAAACCGGGGCAACGCGGAAAATTCGCAGTGAAGCGCGTCACCGGCTTGAGGAAAAACAATGACACAACGTCCATGGAGCAAGTTACAGCGTAAAACGCATAATATTGCTGCGCTAAAAattat        <  1:2269427/150‑1 (MQ=255)
                                                                                                                                                tCTTTCGCTGTTACGTGATTTAGTGAAAACCGGGGCAACGCGGAAAATTCGCAGTGAAGCGCGTCACCGGCTTGAGGAAAAACAATGACACAACGTCCATGGAGCAAGTTACAGCGTAAAACGCATAATATTGCTGCGCTAAAAattatt       <  1:1149774/150‑1 (MQ=255)
                                                                                                                                                 cTTTCGCTGTTACGTGATTTAGTGAAAACCGGGGCAACGCGGAAAATTCGCAGTGAAGCGCGTCACCGGCTTGAGGAAAAACAATGACACAACGTCCATGGAGCAAGTTACAGCGTAAAACGCATAATATTGCTGCGCTAAAAATTATTg      <  1:4367308/150‑1 (MQ=255)
                                                                                                                                                  tttCGCTGTTACGTGATTTAGTGAAAACCGGGGCAACGCGGAAAATTCGCAGTGAAGCGCGTCACCGGCTTGAGGAAAAACAATGACACAACGTCCATGGAGCAAGTTACAGCGTAAAACGCATAATATTGCTGCGCTAAAAATTATTGc     >  2:677578/1‑150 (MQ=255)
                                                                                                                                                   ttCGCTGTTACGTGATTTAGTGAAAACCGGGGCAACGCGGAAAATTCGCAGTGAAGCGCGTCACCGGCTTGAGGAAAAACAATGACACAACGTCCATGGAGCAAGTTACAGCGTAAAACGCATAATATTGCTGCGCTAAAAATTATTGCt    >  2:2864671/1‑150 (MQ=255)
                                                                                                                                                   ttCGCTGTTACGTGATTTAGTGAAAACCGGGGCAACGCGGAAAATTCGCAGTGAAGCGCGTCACCGGCTTGAGGAAAAACAATGACACAACGTCCATGGAGCAAGTTACAGCGTAAAACGCATAATATTGCTGCGCTAAAAATTATTGCt    >  2:3409514/1‑150 (MQ=255)
                                                                                                                                                     cGCTGTTACGTGATTTAGTGAAAACCGGGGCAACGCGGAAAATTCGCAGTGAAGCGCGTCACCGGCTTGAGGAAAAACAATGACACAACGTCCATGGAGCAAGTTACAGCGTAAAACGCATAATATTGCTGCGCTAAAAATTATTGCTCg  <  1:466692/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
ACATACCCCGGCGTTGTTGTTAACGACATTGACTGAGCCTCAGGATCGGTCGCTGGCTATTAATAATCCACAGCTGGCTGCCGATGTGAAAACGGCGTGGTTAAAAGAGGATCCATCATTACTCTTATTTGTCGAACAACCCGATCTTTCGCTGTTACGTGATTTAGTGAAAACCGGGGCAACGCGGAAAATTCGCAGTGAAGCGCGTCACCGGCTTGAGGAAAAACAATGACACAACGTCCATGGAGCAAGTTACAGCGTAAAACGCATAATATTGCTGCGCTAAAAATTATTGCTCG  >  CP009273/404175‑404473

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑