Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 4,382,314 A→T 5.4% noncoding (26/76 nt) glyX → tRNA‑Gly

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092734,382,3140AT 5.4% 520.4 / 10.4 164noncoding (26/76 nt)glyXtRNA‑Gly
Reads supporting (aligned to +/- strand):  ref base A (69/85);  new base T (2/8);  total (71/93)
Fisher's exact test for biased strand distribution p-value = 1.89e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 168 total aligned reads displayed.

ATTAAGTAGTACGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAAGTGCTGCAAAGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCT‑CCAAAATTTGAAAGTGCTGTAAGGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCG  >  CP009273/4382165‑4382463
                                                                                                                                                     |                                                                                                                                                      
aTTAAGTAGTACGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAAGTGCTGCAAAGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCa                                                                                                                                                        >  1:2006861/1‑150 (MQ=255)
   aaGTAGTACGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAATTGCTGCAAAGCACAGAACACAAAAGCGGGAATAGCGCAAATGGTAGAGCACGa                                                                                                                                                     >  2:254459/1‑150 (MQ=14)
      tagtaCGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAAGTGCTGCAAAGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCTCGACCt                                                                                                                                                  <  2:531008/150‑1 (MQ=255)
      tagtaCGCGGGAAGAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAAGTGCTGCAAAGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCt                                                                                                                                                  <  2:2479726/150‑1 (MQ=255)
        gtaCGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAAGTGCTGCAAAGCCCAGACCACCCCAGCGGGAATAGCTCAGTTGGTCTAGCACGACCATg                                                                                                                                                >  1:467229/1‑150 (MQ=14)
               ggAATATCTAATTTTGTAGAGCAAGACCTTTCCAATGTCGGGGTCGCGTTTTCGTGTATCGTTTCCCGCTCCAAAATTTTAAAAGTGCTGCAAAGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGt                                                                                                                                         <  2:1715065/150‑1 (MQ=14)
                gAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAAGTGCTGCAAAGCACAGGCCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTc                                                                                                                                        <  1:2853160/150‑1 (MQ=255)
                 aaTAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAAGTGCTGCAAAGCACAGACCACCCAAGCGGGTATAGCGCAGTTGGTAGAGCTCGCCCGTGCAAAGGTCg                                                                                                                                       >  1:1638620/1‑150 (MQ=14)
                 aaTAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAAGTGCTGCAAAGCACAGACCACCCAAGCGGGACTAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCg                                                                                                                                       >  1:3917725/1‑150 (MQ=255)
                 aaTAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAAGTGCTGCAAAGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCg                                                                                                                                       <  1:241781/150‑1 (MQ=39)
                    aGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAAGTGCTGCAAAGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCgggg                                                                                                                                    <  1:4133128/150‑1 (MQ=39)
                      cTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATGTGAAAACTGCTTAAAAGCACATACCACCCAAGCGGGAATAGCTCGGTTGTTATAGCTCGACCATCCCAAGATCGGGGTc                                                                                                                                  >  2:2940112/1‑150 (MQ=11)
                       tCAGTTGTTAGAGCACGACCTTGCCTAGGTCGGTGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAAGTGCTGCAAAGCACAGACCACCCACGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTcg                                                                                                                                 <  2:3713352/150‑1 (MQ=255)
                             ggTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTATCGTTTCCCGCTCCAAAATTTGAAAAGTGCTGCAAAGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGtt                                                                                                                           <  2:1532799/150‑1 (MQ=255)
                                agagCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAAGTGCTGCAAAGCACAGACCACCCAAGCGGGAATAGCTCGGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGa                                                                                                                        <  2:4228745/150‑1 (MQ=255)
                                agagCACGACCGTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAAGTGCTGCAAAGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGa                                                                                                                        <  2:2006861/150‑1 (MQ=255)
                                    cACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAACTTCGAAAAGTGCTGCCAAGCACAGACCACCCACGCGGGAATAGCTCAGTTGGTAGAGCACGACCTGGCCAAGGTCGGGGTCGCGAGTTCGAGtct                                                                                                                    <  2:1990099/150‑1 (MQ=14)
                                    cACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAAGTGCTGCAAAGCACAGACCACCCCAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGtct                                                                                                                    <  2:3434758/150‑1 (MQ=255)
                                    cACGACCTTGCCAAGGGCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAAGTGCTGCAAAGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGtct                                                                                                                    <  2:3435526/150‑1 (MQ=255)
                                      cGACCTTTCCAAGGTCGGGGTCGCGAGTTCGCGTCTCGTTTCCCGCTCCAAAATTTGAAAAGTGCTGCACAGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCg                                                                                                                  <  2:1511917/150‑1 (MQ=255)
                                          cTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATGTGAAAAGTGATGCAAATCACAGACCACCCAAGCGGGAATAGCTCGGTTGGTATTGCACGACCTTGCAACGGTCGGGGTCGCGAGTTCGAGACTCGTTTc                                                                                                              >  2:57825/1‑150 (MQ=14)
                                            tGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAACTTTGAAAAGTGCTGCAAAGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTccc                                                                                                            <  1:447328/150‑1 (MQ=255)
                                            tGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAAGTGCTGCAAAGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTccc                                                                                                            <  1:2524042/150‑1 (MQ=39)
                                             gCCCAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAAGTGCTGCAAAGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCg                                                                                                           <  1:3880288/150‑1 (MQ=255)
                                                 aGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAAGTGCTGCAAAGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCT‑cc                                                                                                      >  1:2326804/1‑150 (MQ=39)
                                                 aGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAAGTGCTGCAAAGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGGTCGAGTCGCGTTTCCCGCT‑cc                                                                                                      >  1:2326726/1‑150 (MQ=255)
                                                       gggTCGCGAGTTCGAGTCTCGTTTCCCTCTCAAAAATTTGAAAAGTTCTTCAAAGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCT‑CCAAAAtt                                                                                                <  2:3456739/150‑1 (MQ=255)
                                                       gggTCGCGAGTTCGAGTCTCGGTTCCCGCTCCAAAATTTGAAAAGTGCTTCAAAGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCT‑CCAAAAtt                                                                                                <  2:2312405/150‑1 (MQ=255)
                                                        ggTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAAGTGCTGCAAAGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCT‑CCAAAAttt                                                                                               <  1:3688550/150‑1 (MQ=255)
                                                         gTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAAGTGCTTCAAAGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCT‑CCAAAATTTg                                                                                              <  2:1274899/150‑1 (MQ=255)
                                                         gTCGCGAGTTCGAGTCTCGGTTCCCTCTCCAAAATTTGAAAAGTGCTGCAAAGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCT‑CCAAAATTTg                                                                                              <  2:1274845/150‑1 (MQ=255)
                                                           cgcgAGTTCGAGTCTCGTTTCCCTCTCCAAAATTTGAAAATTGCTGCAAAGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCT‑CCAAAATTTGaa                                                                                            <  2:1188479/150‑1 (MQ=255)
                                                           cgcgAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAAGTGCTGCAAAGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCT‑CCAAAATTTGaa                                                                                            <  1:3489059/150‑1 (MQ=255)
                                                             cgAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAAGTGCTGCAAAGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCT‑CCAAAATTTGAAAg                                                                                          <  1:1487638/150‑1 (MQ=255)
                                                               acttCGAGTCTCGGTTCCCGCTCCAAAATGTGAAAAGTGCTCCAAAGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCT‑CCAAAATTTGAAAGTg                                                                                        <  2:1622792/148‑1 (MQ=255)
                                                                    gAGTCTCGTTTCCCGCTCCAAAATTTGAAAAGTTCTGCAAATCACAGACCTCCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCT‑CCAAAATTTGAAAGTGCTGTa                                                                                   <  1:586571/150‑1 (MQ=255)
                                                                      gTCTCGTTTCCCGCTCCAAAATTTGAAAAGTGCTGCAAAGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCT‑CCAAAATTTGAAAGTGCTGTAAg                                                                                 <  1:1192836/150‑1 (MQ=255)
                                                                        ctcGTTTCCCGCTCCAAAATTTGAAAAGTGCTGCAAAGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCT‑CCAAATTTTGAAAGTGCTGTAAGGc                                                                               >  2:1703201/1‑150 (MQ=255)
                                                                        ctcGTTTCCCGCTCCAAAATTTGAAAAGTGCTGCAAAGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCT‑CCAAAATTTGAAAGTGCTGTAAGGc                                                                               <  2:3832476/150‑1 (MQ=255)
                                                                        ctcGTTTCCCGCTCCAAAATTTGAAAAGTGCTGCAAAGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCT‑CCAAAATGTGAAATGCCTGTAAgac                                                                               >  1:548749/1‑148 (MQ=255)
                                                                           gTTTCCCGCTCCAAATTTTGAAAAGTGCTGCAAAGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCT‑CCAAAATTTGAAAGTGCTGTAAGGcaca                                                                            <  1:254459/150‑1 (MQ=255)
                                                                           gTTTCCCGCTCCAAAATTTGAAAAGTGCTTAAAAGCACTGAACACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCT‑CCAAAATTTGAAAGTGCTGTAAGGcaca                                                                            <  1:3846304/150‑1 (MQ=255)
                                                                              tCCCGCTCCAAAATTTGAAAAGTTCTGCAAAGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCT‑CCAAAATTTGAAAGTGCTGTAAGGCACAGac                                                                         <  2:2704897/150‑1 (MQ=255)
                                                                              tCCCGCTCCAAAATTTGAAAAGTGCTGCAAAGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCT‑CCAAAATTTGAAAGTGCTGTAAGGCACAGac                                                                         >  1:1032046/1‑150 (MQ=255)
                                                                              tCCCGCTCAAAAATTTGAAAAGTGCTGCAAATCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCT‑CCAAAATTTGAAAGTGCTGTAAGGCACAGac                                                                         <  2:1160358/150‑1 (MQ=255)
                                                                               cccGCTCCAAAATTTGAAAAGTGCTGCAAAGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCT‑CCAAAATTTGAAAGTGCTGTAAGGCACAGacc                                                                        <  2:915882/150‑1 (MQ=255)
                                                                               cccGCTCCAAAATTTGAAAAGTGCTGCAAAGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCT‑CCAAAATTTGAAAGTGCTGTAAGGCACAGacc                                                                        <  1:2046004/150‑1 (MQ=255)
                                                                                  gCTCCAAAATTTGAAAAGTGCTGCAAAGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCT‑CCAAAATTTGAAAGTGCTGTAATGCACAGaccacc                                                                     >  1:3470229/1‑150 (MQ=255)
                                                                                       aaaaTTTGAAAAGTGCTGCAAAGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCT‑CCAAAATTTGAAAGTGCTGTAAGGCACAGACCACCCAAGc                                                                >  2:4082385/1‑150 (MQ=255)
                                                                                          aTTTGAAAAGTGCTGCAAAGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCT‑CCAAAATTTGAAAGTGCTGTAAGGCACAGACCACCCAAGCggg                                                             >  1:1618683/1‑150 (MQ=255)
                                                                                             tGAAAAGTGCTGCACAGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCTCGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCT‑CCAAAATTTGAAAGTGCTGTTAGGCACAGACCACCCAAGCGGGAAt                                                          <  2:1836482/150‑1 (MQ=255)
                                                                                                  aGTGCTGCAAAGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCT‑CCAAAATTTGAACGTGCTGTAAGGGACAGACCACCCACGCGGGAATAGCtg                                                     >  1:678119/1‑149 (MQ=255)
                                                                                                      ctgcAAAGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCT‑CCAAAATTTGAAAGTGCTGTAAGGCACAGACCACCCACGCGGGAATAGCTCAGtt                                                 >  2:2633511/1‑150 (MQ=255)
                                                                                                      ctgcAAAGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCT‑CCAAAATTTGAAAGTGCTGTAAGGCACAGACCACCCAAGCGGGAATAGCTCAGtt                                                 <  1:1357420/150‑1 (MQ=255)
                                                                                                      ctgcAAAGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCT‑CCAAAATTTGAAAGTGCTGTAAGGCACAGACCACCCAAGCGGGAATAGCTCAGtt                                                 >  1:177246/1‑150 (MQ=255)
                                                                                                      ctgcAAAGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCT‑CCAAAAAGTGAAAGAGCTGTAATGCACAGACCACCCAAGCGGGAAAAGCTCAGtt                                                 >  2:2635502/1‑150 (MQ=17)
                                                                                                          aaaGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCT‑CCAAAATGTTAAAGTGCTGTAAGGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTa                                             >  2:3767463/1‑150 (MQ=255)
                                                                                                           aaGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCT‑CCAAAATTTTAAAGAGCTGAAATTAACACACCAACCAAGCGCGAATTTCTGGGTTGGttg                                            >  1:2117049/1‑148 (MQ=14)
                                                                                                            aGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCT‑CCAAAATTTGAAAGTGATGTAAGGACCAGACCACCCAGGCGGGAATAGCTCAGTTGGTaga                                           >  2:3899680/1‑150 (MQ=255)
                                                                                                             tcacaGACGACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCT‑CCAAAATTTGAAAGTGCTGTAAGGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTagag                                          <  1:1806851/149‑1 (MQ=255)
                                                                                                             gCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCT‑CCAAAATTTGAAAGTGCTGTAAGGCACAGACCACCCAAGCGAGAATAGCTCAGTTGGTagag                                          <  2:1404256/150‑1 (MQ=255)
                                                                                                              cacaGACCACCCAAGCGGGAATAGCTCAGGTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCT‑CCAAAATTTTAAAGAGCTGTAATGCACAGACCACCCAAGCGGGAATAGCGCAGTTGGTataga                                         >  2:79206/1‑149 (MQ=14)
                                                                                                               acaGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCT‑CCAAAATTTGAAAGTGCTGTAAGGGACAGACCACCCACGCGGGAATAGCTCAGGTGGTAGTGCa                                        >  2:3268710/1‑150 (MQ=255)
                                                                                                               acaGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCT‑CCAAAATTTGAAAGTGCTGTAAGGCACAGACCACCCAAGCGGGAATAGCTCGGTTGGTAGAGCa                                        >  2:359733/1‑150 (MQ=255)
                                                                                                                caGACCCCCCACGCGGGAATAGCTCAGTTGGTAGTGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCT‑CCAAAATTTTAAAGTGCTGTAAGGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCAc                                       <  2:4522671/150‑1 (MQ=255)
                                                                                                                 aGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCTCGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCT‑CCAAAATTTGAAAGTGCTGTAAGGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACg                                      <  1:3295210/150‑1 (MQ=255)
                                                                                                                 aGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCT‑CCAAAATTTGAAAGTGCTGTAAGGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACg                                      <  1:4307843/150‑1 (MQ=255)
                                                                                                                 aGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCT‑CCAAAATTTGAAAGTGCTGTAAGGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACg                                      <  1:625336/150‑1 (MQ=255)
                                                                                                                  gACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCT‑CCAAAATTTGAAAGTGCTGTAAGGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGa                                     >  1:3091701/1‑150 (MQ=255)
                                                                                                                   accaccCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCT‑CCAAAATATTATAGAGCTGTAATGGACAGACCACCCAAGCGGGAATAGCTCAGTTGGTagagaaagtc                                    >  2:1321268/1‑144 (MQ=14)
                                                                                                                     caccCACGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCT‑CCAAAATTTGAAAGTGCTGTAAGGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCt                                  <  2:1443869/150‑1 (MQ=255)
                                                                                                                     caccCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCT‑CCAAAATATGAAAGTGCTGTAATGCCCAGACCACCCACGCGGGAATAGCTCAGTTGGTAGAGAACGACCt                                  >  2:3142417/1‑150 (MQ=14)
                                                                                                                      accCAAGCGGGAATAGCTCAGTTGGTAGTGCACGTCCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCT‑CCAAAATATGAAAGTGCTGTAAGGCACAGACCACCCACGCGGGAATAGCTCAGTTGGTAGAGCACGACCtt                                 <  1:381118/150‑1 (MQ=255)
                                                                                                                      accCAAGCGGGAATAGCTCAGTTGGTAGAGCTCGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCT‑CCAAAATTTGAAAGTGCTGTAAGGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCtt                                 <  1:3536774/150‑1 (MQ=255)
                                                                                                                      accCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCT‑CCAAATTTTGAAAGTGATGTAAGGCACAGACCACCCAATCGGGAATAGCTCAGTTGGTAGAGCACGACCtt                                 >  2:3228528/1‑150 (MQ=255)
                                                                                                                             cGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCT‑CCAAAATTTGAAAGTGCTGTAAGGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAgg                          >  1:3970299/1‑150 (MQ=38)
                                                                                                                             cGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCT‑CCAAAATTTGAAAGTGCTGTAAGGCACAGACCACCCAAGCGGGAATAGCTCAGGTGGTAGAGCACGACCTTGCCAAgg                          >  1:168360/1‑150 (MQ=38)
                                                                                                                              gggAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCT‑CCAAAATTTGAAAGTGCTGTAAGGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGt                         <  2:4197128/150‑1 (MQ=38)
                                                                                                                              gggAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCT‑CCAAAATATGAAAGTTCTGTAAGGCACAGACCACCCCAGAGGGAATAGCTCAGTTGGTAGAGCACGACCAAGCCAAGGt                         >  2:4427508/1‑150 (MQ=11)
                                                                                                                              cggAATATCTAATTTGGTAGTGCACTACCTTGCCAAGGTCGGGGTCGCTAGTTCGAGTCTCGTTTCCCGCT‑CCAAAATTTTAAAGTGCTGTAAGGCACATACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGt                         <  2:1714410/149‑1 (MQ=11)
                                                                                                                                gAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCT‑CCAAAATTTGAAAGTGCTGTAAGGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGAcc                                   >  2:713362/1‑138 (MQ=39)
                                                                                                                                gAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCT‑CCAAAATGTGAAAGTGCTGTCAGGCCCAGACCACCCAAGCGGGACTAGCTCAGTTGGTCGAGCACGACCTTGCCACGGTCg                       >  2:1596957/1‑150 (MQ=11)
                                                                                                                                 aaTAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCT‑CCAAAATATGAAAGTGCTGTAATGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCgg                      >  2:3023879/1‑150 (MQ=25)
                                                                                                                                 aaTAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCT‑CCAAAATATGAAAGTGCTGTAATGAACAGACCACCCAAGCGGGAAAAGCGAAGTTGGTAGAGAACGACCTTCCCACGGTCgg                      >  2:3023828/1‑150 (MQ=11)
                                                                                                                                 aaTAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCT‑CCAAAATATGAAAGTGATGTAAGGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTAGACAAGGTCgg                      >  2:281920/1‑150 (MQ=255)
                                                                                                                                   tAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCT‑CCAAAATTTGAAAGTGCTGTAAGGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCgggg                    <  1:1840484/150‑1 (MQ=38)
                                                                                                                                     gCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCT‑CCAAAATTTGAAAGTGCTGTAAGGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTc                  <  2:797221/150‑1 (MQ=38)
                                                                                                                                      cTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCT‑CCAAAATTTGAAAGTGCTGTAAGGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTATAGCACGACCTTGCCAAGGTCGGGGTcg                 >  2:2991958/1‑150 (MQ=38)
                                                                                                                                         aGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCT‑CCAAAATTTGAAAGTGCTGTAAGTCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGa              >  2:621117/1‑150 (MQ=38)
                                                                                                                                         aGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCT‑CCAAAATTTGAAAGTGCTGTAAGGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGa              >  1:4537212/1‑150 (MQ=38)
                                                                                                                                            tGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCT‑CCAAAATTTGAAAGTGCTGTAAGGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGtt           <  1:4355749/150‑1 (MQ=38)
                                                                                                                                             ggTAGAGCACGACCTGGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCC‑CCAAAATATGAAAGTGCTGTAAGGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTc          <  1:154984/150‑1 (MQ=255)
                                                                                                                                             ggTAGAGCACGACCTCGCCAAGGTCGGGGCCGCGAGTTCGAGTCCCGTTTCCCGCT‑CCAAAATTTGAAAGTGCTGTAAGGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTc          <  2:848506/150‑1 (MQ=255)
                                                                                                                                               tAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCT‑CCAAAATTTGAAAGTGCTGTAAGGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGAACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGa        >  2:619416/1‑150 (MQ=38)
                                                                                                                                                agagCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCT‑CCAAAATTTGAAAGTGCTGTAAGGCACAGACCACCCAATCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAATGTCGGGGTCGCGAGTTCGAg       >  1:1270774/1‑150 (MQ=255)
                                                                                                                                                    cACGTCCTTGGCAACGTCGGGGTCGCGTTTTCCCCGTACCTTTACCGCTCCCAAAATTGTAAAGTGCTGGAAGGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGtct    <  2:2078360/150‑1 (MQ=1)
                                                                                                                                                    cACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCT‑CCAAAATTTGAAAGTGCTGTAAGGCACAGACCACCCAAGCGGGAATATCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGtctc   >  2:3263104/1‑150 (MQ=38)
                                                                                                                                                    cACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCT‑CCAAAATTTGAAAGTGCTGTAAGGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGtctc   >  1:3031454/1‑150 (MQ=38)
                                                                                                                                                    cACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCT‑CCAAAATTTGAAAGTGCTGTAAGGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGtctc   >  1:772065/1‑150 (MQ=38)
                                                                                                                                                     aCGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCT‑CCAAAATTTGAAAGTGCTGTAAGGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCg  >  2:3177095/1‑150 (MQ=38)
                                                                                                                                                     |                                                                                                                                                      
ATTAAGTAGTACGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAAGTGCTGCAAAGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCT‑CCAAAATTTGAAAGTGCTGTAAGGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCG  >  CP009273/4382165‑4382463

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑