Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 4,459,683 A→T 11.9% N748Y (AAC→TAC)  mgtA → magnesium transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092734,459,6830AT11.9% 802.6 / 77.6 250N748Y (AAC→TAC) mgtAmagnesium transporter
Reads supporting (aligned to +/- strand):  ref base A (108/112);  new base T (17/13);  total (125/125)
Fisher's exact test for biased strand distribution p-value = 5.60e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 252 total aligned reads displayed.

CATCAAAATGACGGCGAGCTCTAACTTCGGTAATGTGTTCAGCGTGCTGGTAGCGAGTGCTTTCTTGCCCTTCCTGCCGATGTTGCCGTTACACTTGCTTATTCAGAACCTGCTGTACGATGTGTCACAGGTGGCGATCCCGTTTGATAACGTCGACGACGAGCAAATTCAAAAGCCGCAGCGTTGGAATCCGGCGGATCTGGGGCGCTTTATGATCTTCTTCGGACCGATCAGCTCGATCTTCGATATTTTGACGTTTTGCCTGATGTGGTGGGTATTCCATGCCAACACGCCGGAA  >  CP009273/4459535‑4459832
                                                                                                                                                    |                                                                                                                                                     
cATCAAAATGACGGCGAGCTCTAACTTCGGTAATGTGTTCAGCGTGCTGGTAGCGAGTGCTTTCTTGCCCTTCCTGCCGATGTTGCCGTTACACTTGCTTATTCAGAACCTGCTGTACGATGTGTCACAGGTGGCGATCCCGTTTGATaa                                                                                                                                                      <  1:3716452/150‑1 (MQ=255)
 aTCAAAATGACGGCGAGCTCTAACTTCGGTAATGTGTTCAGCGTGCTGGTAGCGAGTGCTTTCTTGCCCTTCCTGCCGATGTTGCCGTTACACTTGCTTATTCAGAACCTGCTGTACGATGTGTCACAGGTGGCGATCCCGTTTGATAAc                                                                                                                                                     <  1:3365393/150‑1 (MQ=255)
  tCAAAATGACGGCGAGCTCTAACTTCGGTAATGTGTTCAGCGTGCTGGTAGCGAGTGCTTTCTTGCCCTTCCTGCCGATGTTGCCGTTACACTTGCTTATTCAGAACCTGCTGTACGATGTGTCACAGGTGGCGATCCCGTTTGATTACg                                                                                                                                                    <  1:1208409/150‑1 (MQ=255)
    aaaaTGACGGCGAGCTCTAACTTCGGTAATGTGTTCAGCGTGCTGGTAGCGAGTGCTTTCTTGCCCTTCCTGCCGATGTTGCCGTTACACTTGCTTATTCAGAACCTGCTGTACGATGTGTCACAGGTGGCGATCCCGTTTGATAACGTc                                                                                                                                                  <  1:2785934/150‑1 (MQ=255)
    aaaaTGACGGCGAGCTCTAACTTCGGTAATGTGTTCAGCGTGCTGGTAGCGAGTGCTTTCTTGCCCTTCCTGCCGATGTTGCCGTTACACTTGCTTATTCAGAACCTGCTGTACGATGTGTCACAGGTGGCGATCCCGTTTGATAACGTc                                                                                                                                                  <  1:2365773/150‑1 (MQ=255)
     aaaTGACGGCGAGCTCTAACTTCGGTAATGTGTTCAGCGTGCTGGTAGCGAGTGCTTTCTTGCCCTTCCTGCCGATGTTGCCGTTACACTTGCTTATTCAGAACCTGCTGTACGATGTGTCACAGGTGGCGATCCCGTTTGATTACGTcg                                                                                                                                                 >  2:1603197/1‑150 (MQ=255)
     aaaTGACGGCGAGCTCTAACTTCGGTAATGTGTTCAGCGTGCTGGTAGCGAGTGCTTTCTTGCCCTTCCTGCCGATGTTGCCGTTACACTTGCTTATTCAGAACCTGCTGTACGATGTGTCACAGGTGGCGATCCCGTTTGATAACGTcg                                                                                                                                                 <  1:2537194/150‑1 (MQ=255)
     aaaTGACGGCGAGCTCTAACTTCGGTAATGTGTTCAGCGTGCTGGTAGCGAGTGCTTTCTTGCCCTTCCTGCCGATGTTGCCGTTACACTTGCTTATTCAGAACCTGCTGTACGATGTGTCACAGGTGGCGATCCCGTTTGATAACGTcg                                                                                                                                                 <  2:2037416/150‑1 (MQ=255)
       aTGACGGCGAGCTCTAACTTCGGTAATGTGTTCAGCGTGCTGGTAGCGAGTGCTTTCTTGCCCTTCCTGCCGATGTTGCCGTTACACTTGCTTATTCAGAACCTGCTGTACGATGTGTCACAGGTGGCGATCCCGTTTGATTACGTcgac                                                                                                                                               >  2:4280174/1‑150 (MQ=255)
          aCGGCGAGCTCTAACTTCGGTAATGTGTTCAGCGTGCTGGTAGCGAGTGCTTTCTTGCCCTTCCTGCCGATGTTGCCGTTACACTTGCTTATTCAGAACCTGCTGTACGATGTGTCACAGGTGGCGATCCCGTTTGATAACGTcgacgac                                                                                                                                            >  2:2929507/1‑150 (MQ=255)
              cGAGCTCTAACTTCGGTAATGTGTTCAGCGTGCTGGTAGCGAGTGCTTTCTTGCCCTTCCTGCCGATGTTGCCGTTACACTTGCTTATTCAGAACCTGCTGTACGATGTGTCACAGGTGGCGATCCCGTTTGATAACGTCGACGACGAGc                                                                                                                                        >  2:3090419/1‑150 (MQ=255)
               gAGCTCTAACTTCGGTAATGTGTTCAGCGTGCTGGTAGCGAGTGCTTTCTTGCCCTTCCTGCCGATGTTGCCGTTACACTTGCTTATTCAGAACCTGCTGTACGATGTGTCACAGGTGGCGATCCCGTTTGATTACGTCGACGACGAGCa                                                                                                                                       >  1:3524735/1‑150 (MQ=255)
               gAGCTCTAACTTCGGTAATGTGTTCAGCGTGCTGGTAGCGAGTGCTTTCTTGCCCTTCCTGCCGATGTTGCCGTTACACTTGCTTATTCAGAACCTGCTGTACGATGTGTCACAGGTGGCGATCCCGTTTGATAACGTCGACGACGAGCa                                                                                                                                       >  2:3286420/1‑150 (MQ=255)
                aGCTCTAACTTCGGTAATGTGTTCAGCGTGCTGGTAGCGAGTGCTTTCTTGCCCTTCCTGCCGATGTTGCCGTTACACTTGCTTATTCAGAACCTGCTGTACGATGTGTCACAGGTGGCGATCCCGTTTGATAACGTCGACGACGAGCaa                                                                                                                                      >  1:3664778/1‑150 (MQ=255)
                aGCTCTAACTTCGGTAATGTGTTCAGCGTGCTGGTAGCGAGTGCTTTCTTGCCCTTCCTGCCGATGTTGCCGTTACACTTGCTTATTCAGAACCTGCTGTACGATGTGTCACAGGTGGCGATCCCGTTTGATAACGTCGACGACGAGCaa                                                                                                                                      <  1:2086602/150‑1 (MQ=255)
                  ctctAACTTCGGTAATGTGTTCAGCGTGCTGGTAGCGAGTGCTTTCTTGCCCTTCCTGCCGATGTTGCCGTTACACTTGCTTATTCAGAACCTGCTGTACGATGTGTCACAGGTGGCGATCCCGTTTGATTACGTCGACGACGAGCAAAt                                                                                                                                    >  2:1452572/1‑150 (MQ=255)
                  ctctAACTTCGGTAATGTGTTCAGCGTGCTGGTAGCGAGTGCTTTCTTGCCCTTCCTGCCGATGTTGCCGTTACACTTGCTTATTCAGAACCTGCTGTACGATGTGTCACAGGTGGCGATCCCGTTTGATAACGTCGACGACGAGCAAAt                                                                                                                                    >  2:1558465/1‑150 (MQ=255)
                  ctctAACTTCGGTAATGTGTTCAGCGTGCTGGTAGCGAGTGCTTTCTTGCCCTTCCTGCCGATGTTGCCGTTACACTTGCTTATTCAGAACCTGCTGTACGATGTGTCACAGGTGGCGATCCCGTTTGATAACGTCGACGACGAGCAAAt                                                                                                                                    >  2:480498/1‑150 (MQ=255)
                   tctAACTTCGGTAATGTGTTCAGCGTGCTGGTAGCGAGTGCTTTCTTGCCCTTCCTGCCGATGTTGCCGTTACACTTGCTTATTCAGAACCTGCTGTACGATGTGTCACAGGTGGCGATCCCGTTTGATAACGTCGACGACGAGCAAAtt                                                                                                                                   >  2:782449/1‑150 (MQ=255)
                    ctAACTTCGGTAATGTGTTCAGCGTGCTGGTAGCGAGTGCTTTCTTGCCCTTCCTGCCGATGTTGCCGTTACACTTGCTTATTCAGAACCTGCTGTACGATGTGTCACAGGTGGCGATCCCGTTTGATAACGTCGACGACGAGCAAATTc                                                                                                                                  <  2:368541/150‑1 (MQ=255)
                     tAACTTCGGTAATGTGTTCAGCGTGCTGGTAGCGAGTGCTTTCTTGCCCTTCCTGCCGATGTTGCCGTTACACTTGCTTATTCAGAACCTGCTGTACGATGTGTCACAGGTGGCGATCCCGTTTGATAACGTCGACGACGAGCAAATTCa                                                                                                                                 >  2:4108480/1‑150 (MQ=255)
                         ttCGGTAATGTGTTCAGCGTGCTGGTAGCGAGTGCTTTCTTGCCCTTCCTGCCGATGTTGCCGTTACACTTGCTTATTCAGAACCTGCTGTACGATGTGTCACAGGTGGCGATCCCGTTTGATAACGTCGACGACGAGCAAATTCAAAAg                                                                                                                             >  2:2207366/1‑150 (MQ=255)
                           cGGTAATGTGTTCAGCGTGCTGGTAGCGAGTGCTTTCTTGCCCTTCCTGCCGATGTTGCCGTTACACTTGCTTATTCAGAACCTGCTGTACGATGTGTCACAGGTGGCGATCCCGTTTGATAACGTCGACGACGAGCAAATTCAAAAGcc                                                                                                                           <  1:2639731/150‑1 (MQ=255)
                            ggTAATGTGTTCAGCGTGCTGGTAGCGAGTGCTTTCTTGCCCTTCCTGCCGATGTTGCCGTTACACTTGCTTATTCAGAACCTGCTGTACGATGTGTCACAGGTGGCGATCCCGTTTGATTACGTCGACGACGAGCAAATTCAAAAGCCg                                                                                                                          >  2:3667655/1‑150 (MQ=255)
                            ggTAATGTGTTCAGCGTGCTGGTAGCGAGTGCTTTCTTGCCCTTCCTGCCGATGTTGCCGTTACACTTGCTTATTCAGAACCTGCTGTACGATGTGTCACAGGTGGCGATCCCGTTTGATAACGTCGACGACGAGCAAATTCAAAAGCCg                                                                                                                          <  2:2490867/150‑1 (MQ=255)
                              tAATGTGTTCAGCGTGCTGGTAGCGAGTGCTTTCTTGCCCTTCCTGCCGATGTTGCCGTTACACTTGCTTATTCAGAACCTGCTGTACGATGTGTCACAGGTGGCGATCCCGTTTGATAACGTCGACGACGAGCAAATTCAAAAGCCGCa                                                                                                                        >  1:242830/1‑150 (MQ=255)
                              tAATGTGTTCAGCGTGCTGGTAGCGAGTGCTTTCTTGCCCTTCCTGCCGATGTTGCCGTTACACTTGCTTATTCAGAACCTGCTGTACGATGTGTCACAGGTGGCGATCCCGTTTGATAACGTCGACGACGAGCAAATTCAAAAGCCGCa                                                                                                                        >  2:3919382/1‑150 (MQ=255)
                               aaTGTGTTCAGCGTGCTGGTAGCGAGTGCTTTCTTGCCCTTCCTGCCGATGTTGCCGTTACACTTGCTTATTCAGAACCTGCTGTACGATGTGTCACAGGTGGCGATCCCGTTTGATAACGTCGACGACGAGCAAATTCAAAAGCCGCAg                                                                                                                       <  1:3438881/150‑1 (MQ=255)
                               aaTGTGTTCAGCGTGCTGGTAGCGAGTGCTTTCTTGCCCTTCCTGCCGATGTTGCCGTTACACTTGCTTATTCAGAACCTGCTGTACGATGTGTCACAGGTGGCGATCCCGTTTGATAACGTCGACGACGAGCAAATTCAAAAGCCGCAg                                                                                                                       <  1:176708/150‑1 (MQ=255)
                                aTGTGTTCAGCGTGCTGGTAGCGAGTGCTTTCTTGCCCTTCCTGCCGATGTTGCCGTTACACTTGCTTATTCAGAACCTGCTGTACGATGTGTCACAGGTGGCGATCCCGTTTGATAACGTCGACGACGAGCAAATTCAAAAGCCGCAGc                                                                                                                      >  2:595049/1‑150 (MQ=255)
                                     ttCAGCGTGCTGGTAGCGAGTGCTTTCTTGCCCTTCCTGCCGATGTTGCCGTTACACTTGCTTATTCAGAACCTGCTGTACGATGTGTCACAGGTGGCGATCCCGTTTGATAACGTCGACGACGAGCAAATTCAAAAGCCGCAGCGTTgg                                                                                                                 >  1:2135624/1‑150 (MQ=255)
                                      tCAGCGTGCTGGTAGCGAGTGCTTTCTTGCCCTTCCTGCCGATGTTGCCGTTACACTTGCTTATTCAGAACCTGCTGTACGATGTGTCACAGGTGGCGATCCCGTTTGATAACGTCGACGACGAGCAAATTCAAAAGCCGCAGCGTTGGa                                                                                                                <  1:4028852/150‑1 (MQ=255)
                                        aGCGTGCTGGTAGCGAGTGCTTTCTTGCCCTTCCTGCCGATGTTGCCGTTACACTTGCTTATTCAGAACCTGCTGTACGATGTGTCACAGGTGGCGATCCCGTTTGATAACGTCGACGACGAGCAAATTCAAAAGCCGCAGCGTTGGAAt                                                                                                              >  1:644460/1‑150 (MQ=255)
                                           gTGCTGGTAGCGAGTGCTTTCTTGCCCTTCCTGCCGATGTTGCCGTTACACTTGCTTATTCAGAACCTGCTGTACGATGTGTCACAGGTGGCGATCCCGTTTGATAACGTCGACGACGAGCAAATTCAAAAGCCGCAGCGTTGGAATCcg                                                                                                           >  2:2150699/1‑150 (MQ=255)
                                           gTGCTGGTAGCGAGTGCTTTCTTGCCCTTCCTGCCGATGTCGCCGTTACACTTGCTTATTCAGAACCTGCTGTACGATGTGTCACAGGTGGCGATCCCGTTTGATAACGTCGACGACGAGCAAATTCAAAAGCCGCAGCGTTGGAATCcg                                                                                                           >  2:2150664/1‑150 (MQ=255)
                                               tGGTAGCGAGTGCTTTCTTGCCCTTCCTGCCGATGTTGCCGTTACACTTGCTTATTCAGAACCTGCTGTACGATGTGTCACAGGTGGCGATCCCGTTTGATAACGTCGACGACGAGCAAATTCAAAAGCCGCAGCGTTGGAATCcggcgg                                                                                                       >  1:1646373/1‑150 (MQ=255)
                                                    gCGAGTGCTTTCTTGCCCTTCCTGCCGATGTTGCCGTTACACTTGCTTATTCAGAACCTGCTGTACGTTGTGTCACAGGTGGCGATCCCGTTTGATAACGTCGACGACGAGCAAATTCAAAAGCCGCAGCGTTGGAATCCGGCGGATCTg                                                                                                  <  1:202844/150‑1 (MQ=255)
                                                     cGAGTGCTTTCTTGCCCTTCCTGCCGATGTTGCCGTTACACTTGCTTATTCAGAACCTGCTGTACGATGTGTCACAGGTGGCGATCCCGTTTGATAACGTCGACGACGAGCAAATTCAAAAGCCGCAGCGTTGGAATCCGGCGGATCTgg                                                                                                 <  1:3778299/150‑1 (MQ=255)
                                                        gTGCTTTCTTGCCCTTCCTGCCGATGTTGCCGTTACACTTGCTTATTCAGAACCTGCTGTACGATGTGTCACAGGTGGCGATCCCGTTTGATAACGTCGACGACGAGCAAATTCAAAAGCCGCAGCGTTGGAATCCGGCGGATCTGGGgc                                                                                              <  2:323393/150‑1 (MQ=255)
                                                        gTGCTTTCTTGCCCTTCCTGCCGATGTTGCCGTTACACTTGCTTATTCAGAACCTGCTGTACGATGTGTCACAGGTGGCGATCCCGTTTGATAACGTCGACGACGAGCAAATTCAAAAGCCGCAGCGTTGGAATCCGGCGGATCTGGGgc                                                                                              >  1:1934308/1‑150 (MQ=255)
                                                        gTGCTTTCTTGCCCTTCCTGCCGATGTTGCCGTTACACTTGCTTATTCAGAACCTGCTGTACGATGTGTCACAGGTGGCGATCCCGTTTGATAACGTCGACGACGAGCAAATTCAAAAGCCGCAGCGTTGGAATCCGGCGGATCTGGGgc                                                                                              <  2:323678/150‑1 (MQ=255)
                                                         tGCTTTCTTGCCCTTCCTGCCGATGTTGCCGTTACACTTGCTTATTCAGAACCTGCTGTACGATGTGTCACAGGTGGCGATCCCGTTTGATTACGTCGACGACGAGCAAATTCAAAAGCCGCAGCGTTGGAATCCGGCGGATCTGGGgcg                                                                                             <  1:4493050/150‑1 (MQ=255)
                                                              tcgtGCCCTTCCTGCCGATGTTGCCGTTACACTTGCTTATTCAGAACCTGCTGTACGATGTGTCACAGGTGGCGATCCCGTTTGATAACGTCGACGACGAGCAAATTCAAAAGCCGCAGCGTTGGAATCCGGCGGATCTGGGGCGCTTTa                                                                                        <  2:889054/147‑1 (MQ=255)
                                                              tCTTGCCCTTCCTGCCGATGTTGCCGTTACACTTGCTTATTCAGAACCTGCTGTACGATGTGTCACAGGTGGCGATCCCGTTTGATAACGTCGACGACGAGCAAATTCAAAAGCCGCAGCGTTGGAATCCGGCGGATCTGGGGCGCTTTa                                                                                        <  1:740535/150‑1 (MQ=255)
                                                              tCTTGCCCTTCCTGCCGATGTTGCCGTTACACTTGCTTATTCAGAACCTGCTGTACGATGTGTCACAGGTGGCGATCCCGTTTGATAACGTCGACGACGAGCAAATTCAAAAGCCGCAGCGTTGGAATCCGGCGGATCTGGGGCGCTTTa                                                                                        <  1:1850322/150‑1 (MQ=255)
                                                               cTTGCCCTTCCTGCCGATGTTGCCGTTACACTTGCTTATTCAGAACCTGCTGTACGATGTGTCACAGGTGGCGATCCCGTTTGATAACGTCGACGACGAGCAAATTCAAAAGCCGCAGCGTTGGAATCCGGCGGATCTGGGGCGCTTTAt                                                                                       >  1:722029/1‑150 (MQ=255)
                                                                 tGCCCTTCCTGCCGATGTTGCCGTTACACTTGCTTATTCAGAACCTGCTGTACGATGTGTCACAGGTGGCGATCCCGTTTGATTACGTCGACGACGAGCAAATTCAAAAGCCGCAGCGTTGGAATCCGGCGGATCTGGGGCGCTTTATGa                                                                                     >  1:610770/1‑150 (MQ=255)
                                                                 tGCCCTTCCTGCCGATGTTGCCGTTACACTTGCTTATTCAGAACCTGCTGTACGATGTGTCACAGGTGGCGATCCCGTTTGATAACGTCGACGACGAGCAAATTCAAAAGCCGCAGCGTTGGAATCCGGCGGATCTGGGGCGCTTTATGa                                                                                     <  1:1405519/150‑1 (MQ=255)
                                                                  gCCCTTCCTGCCGATGTTGCCGTTACACTTGCTTATTCAGAACCTGCTGTACGATGTGTCACAGGTGGCGATCCCGTTTGATAACGTCGACGACGAGCAAATTCAAAAGCCGCAGCGTTGGAATCCGGCGGATCTGGGGCGCTTTATGAt                                                                                    <  1:1077112/150‑1 (MQ=255)
                                                                  gCCCTTCCTGCCGATGTTGCCGTTACACTTGCTTATTCAGAACCTGCTGTACGATGTGTCACAGGTGGCGATCCCGTTTGATAACGTCGACGACGAGCAAATTCAAAAGCCGCAGCGTTGGAATCCGGCGGATCTGGGGCGCTTTATGAt                                                                                    >  1:1468616/1‑150 (MQ=255)
                                                                   cccTTCCTGCCGATGTTGCCGTTACACTTGCTTATTCAGAACCTGCTGTACGATGTGTCACAGGTGGCGATCCCGTTTGATTACGTCGACGACGAGCAAATTCAAAAGCCGCAGCGTTGGAATCCGGCGGATCTGGGGCGCTTTATGAtc                                                                                   >  1:3504573/1‑150 (MQ=255)
                                                                    ccTTCCTGCCGATGTTGCCGTTACACTTGCTTATTCAGAACCTGCTGTACGATGTGTCACAGGTGGCGATCCCGTTTGATAACGTCGACGACGAGCAAATTCAAAAGCCGCAGCGTTGGAATCCGGCGGATCTGGGGCGCTTTATGAtct                                                                                  <  1:3646475/150‑1 (MQ=255)
                                                                     cTTCCTGCCGATGTTGCCGTTACACTTGCTTATTCAGAACCTGCTGTACGATGTGTCACAGGTGGCGATCCCGTTTGATAACGTCGACGACGAGCAAATTCAAAAGCCGCAGCGTTGGAATCCGGCGGATCTGGGGCGCTTTATGAtctt                                                                                 <  1:1405146/150‑1 (MQ=255)
                                                                       tCCTGCCGATGTTGCCGTTACACTTGCTTATTCAGAACCTGCTGTACGATGTGTCACAGGTGGCGATCCCGTTTGATAACGTCGACGACGAGCAAATTCAAAAGCCGCAGCGTTGGAATCCGGCGGATCTGGGGCGCTTTATGAtcttct                                                                               <  1:107579/150‑1 (MQ=255)
                                                                       tCCTGCCGATGTTGCCGTTACACTTGCTTATTCAGAACCTGCTGTACGATGTGTCACAGGTGGCGATCCCGTTTGATAACGTCGACGACGAGCAAATTCAAAAGCCGCAGCGTTGGAATCCGGCGGATCTGGGGCGCTTTATGAtcttct                                                                               <  1:4054487/150‑1 (MQ=255)
                                                                       tCCTGCCGATGTTGCCGTTACACTTGCTTATTCAGAACCTGCTGTACGATGTGTCACAGGTGGCGATCCCGTTTGATAACGTCGACGACGAGCAAATTCAAAAGCCGCAGCGTTGGAATCCGGCGGATCTGGGGCGCTTTATGAtcttct                                                                               <  1:1462271/150‑1 (MQ=255)
                                                                         cTGCCGATGTTGCCGTTACACTTGCTTATTCAGAACCTGCTGTACGATGTGTCACAGGTGGCGATCCCGTTTGATTACGTCGACGACGAGCAAATTCAAAAGCCGCAGCGTTGGAATCCGGCGGATCTGGGGCGCTTTATGATCttcttc                                                                             <  2:1198630/150‑1 (MQ=255)
                                                                          tGCCGATGTTGCCGTTACACTTGCTTATTCAGAACCTGCTGTACGATGTGTCACAGGTGGCGATCCCGTTTGATAACGTCGACGACGAGCAAATTCAAAAGCCGCAGCGTTGGAATCCGGCGGATCTGGGGCGCTTTATGATCTTCTTCg                                                                            <  2:4352531/150‑1 (MQ=255)
                                                                          tGCCGATGTTGCCGTTACACTTGCTTATTCAGAACCTGCTGTACGATGTGTCACAGGTGGCGATCCCGTTTGATAACGTCGACGACGAGCAAATTCAAAAGCCGCAGCGTTGGAATCCGGCGGATCTGGGGCGCTTTATGATCTTCTTCg                                                                            >  2:379052/1‑150 (MQ=255)
                                                                               aTGTTGCCGTTACACTTGCTTATTCAGAACCTGCTGTACGATGTGTCACAGGTGGCGATCCCGTTTGATAACGTCGACGACGAGCAAATTCAAAAGCCGCAGCGTTGGAATCCGGCGGATCTGGGGCGCTTTATGATCTTCTTCGGACCg                                                                       <  2:4243155/150‑1 (MQ=255)
                                                                               aTGTTGCCGTTACACTTGCTTATTCAGAACCTGCTGTACGATGTGTCACAGGTGGCGATCCCGTTTGATAACGTCGACGACGAGCAAATTCAAAAGCCGCAGCGTTGGAATCCGGCGGATCTGGGGCGCTTTATGATCTTCTTCGGACCg                                                                       <  1:3174323/150‑1 (MQ=255)
                                                                                tGTTGCCGTTACACTTGCTTATTCAGAACCTGCTGTACGATGTGTCACAGGTGGCGATCCCGTTTGATAACGTCGACGACGAGCAAATTCAAAAGCCGCAGCGTTGGAATCCGGCGGATCTGGGGCGCTTTATGATCTTCTTCGGACCGa                                                                      >  2:1158188/1‑150 (MQ=255)
                                                                                 gTTGCCGTTACACTTGCTTATTCAGAACCTGCTGTACGATGTGTCACAGGTGGCGATCCCGTTTGATAACGTCGACGACGAGCAAATTCAAAAGCCGCAGCGTTGGAATCCGGCGGATCTGGGGCGCTTTATGATCTTCTTCGGACCGAt                                                                     >  1:3693097/1‑150 (MQ=255)
                                                                                  ttGCCGTTACACTTGCTTATTCAGAACCTGCTGTACGATGTGTCACAGGTGGCGATCCCGTTTGATTACGTCGACGACGAGCAAATTCAAAAGCCGCAGCGTTGGAATCCGGCGGATCTGGGGCGCTTTATGATCTTCTTCGGACCGATc                                                                    <  1:328892/150‑1 (MQ=255)
                                                                                   tGCCGTTACACTTGCTTATTCAGAACCTGCTGTACGATGTGTCACAGGTGGCGATCCCGTTTGATAACGTCGACGACGAGCAAATTCAAAAGCCGCAGCGTTGGAATCCGGCGGATCTGGGGCGCTTTATGATCTTCTTCGGACCGATCa                                                                   >  2:1032528/1‑150 (MQ=255)
                                                                                   tGCCGTTACACTTGCTTATTCAGAACCTGCTGTACGATGTGTCACAGGTGGCGATCCCGTTTGATAACGTCGACGACGAGCAAATTCAAAAGCCGCAGCGTTGGAATCCGGCGGATCTGGGGCGCTTTATGATCTTCTTCGGACCGATCa                                                                   >  2:3987166/1‑150 (MQ=255)
                                                                                   tGCCGTTACACTTGCTTATTCAGAACCTGCTGTACGATGTGTCACAGGTGGCGATCCCGTTTGATAACGTCGACGACGAGCAAATTCAAAAGCCGCAGCGTTGGAATCCGGCGGATCTGGGGCGCTTTATGATCTTCTTCGGACCGATCa                                                                   >  2:630653/1‑150 (MQ=255)
                                                                                     ccGTTACACTTGCTTATTCAGAACCTGCTGTACGATGTGTCACAGGTGGCGATCCCGTTTGATAACGTCGACGACGAGCAAATTCAAAAGCCGCAGCGTTGGAATCCGGCGGATCTGGGGCGCTTTATGATCTTCTTCGGACCGATCAGc                                                                 >  1:675021/1‑150 (MQ=255)
                                                                                          acacTTGCTTATTCAGAACCTGCTGTACGATGTGTCACAGGTGGCGATCCCGTTTGATAACGTCGACGACGAGCAAATTCAAAAGCCGCAGCGTTGGAATCCGGCGGATCTGGGGCGCTTTATGATCTTCTTCGGACCGATCAGCTCGAt                                                            >  2:2691847/1‑150 (MQ=255)
                                                                                             cTTGCTTATTCAGAACCTGCTGTACGATGTGTCACTGGTGGCGATCCCGTTTGATAACGTCGACGACGAGCAAATTCAAAAGCCGCAGCGTTGGAATCCGGCGGATCTGGGGCGCTTTATGATCTTCTTCGGACCGATCAGCTCGATCtt                                                         <  1:3920514/150‑1 (MQ=255)
                                                                                               tGCTTATTCAGAACCTGCTGTACGATGTGTCACAGGTGGCGATCCCGTTTGATAACGTCGACGACGAGCAAATTCAAAAGCCGCAGCGTTGGAATCCGGCGGATCTGGGGCGCTTTATGATCTTCTTCGGACCGATCAGCTCGATCTTCg                                                       <  2:1410819/150‑1 (MQ=255)
                                                                                               tGCTTATTCAGAACCTGCTGTACGATGTGTCACAGGTGGCGATCCCGTTTGATAACGTCGACGACGAGCAAATTCAAAAGCCGCAGCGTTGGAATCCGGCGGATCTGGGGCGCTTTATGATCTTCTTCGGACCGATCAGCTCGATCTTCg                                                       <  2:3851329/150‑1 (MQ=255)
                                                                                                    aTTCAGAACCTGCTGTACGATGTGTCACAGGTGGCGATCCCGTTTGATAACGTCGACGACGAGCAAATTCAAAAGCCGCAGCGTTGGAATCCGGCGGATCTGGGGCGCTTTATGATCTTCTTCGGACCGATCAGCTCGATCTTCGATAtt                                                  <  2:4255881/150‑1 (MQ=255)
                                                                                                    aTTCAGAACCTGCTGTACGATGTGTCACAGGTGGCGATCCCGTTTGATAACGTCGACGACGAGCAAATTCAAAAGCCGCAGCGTTGGAATCCGGCGGATCTGGGGCGCTTTATGATCTTCTTCGGACCGATCAGCTCGATCTTCGATAtt                                                  >  2:3574949/1‑150 (MQ=255)
                                                                                                    aTTCAGAACCTGCTGTACGATGTGTCACAGGTGGCGATCCCGTTTGATAACGTCGACGACGAGCAAATTCAAAAGCCGCAGCGTTGGAATCCGGCGGATCTGGGGCGCTTTATGATCTTCTTCGGACCGATCAGCTCGATCTTCGATAtt                                                  >  2:2581743/1‑150 (MQ=255)
                                                                                                    aTTCAGAACCTGCTGTACGATGTGTCACAGGTGGCGATCCCGTTTGATAACGTCGACGACGAGCAAATTCAAAAGCCGCAGCGTTGGAATCCGGCGGATCTGGGGCGCTTTATGATCTTCTTCGGACCGATCAGCTCGATCTTCGATAtt                                                  <  1:2051202/150‑1 (MQ=255)
                                                                                                    aTTCAGAACCTGCTGTACGATGTGTCACAGGTGGCGATCCCGTTTGATAACGTCGACGACGAGCAAATTCAAAAGCCGCAGCGTTGGAATCCGGCGGATCTGGGGCGCTTTATGATCTTCTTCGGACCGATCAGCTCGATCTTCGATAtt                                                  <  1:4326368/150‑1 (MQ=255)
                                                                                                      tCAGAACCTGCTGTACGATGTGTCACAGGTGGCGATCCCGTTTGATAACGTCGACGACGAGCAAATTCAAAAGCCGCAGCGTTGGAATCCGGCGGATCTGGGGCGCTTTATGATCTTCTTCGGACCGATCAGCTCGATCTTCGATAtttt                                                <  1:4248702/150‑1 (MQ=255)
                                                                                                      tCAGAACCTGCTGTACGATGTGTCACAGGTGGCGATCCCGTTTGATAACGTCGACGACGAGCAAATTCAAAAGCCGCAGCGTTGGAATCCGGCGGATCTGGGGCGCTTTATGATCTTCTTCGGACCGATCAGCTCGATCTTCGATAtttt                                                <  2:723521/150‑1 (MQ=255)
                                                                                                      tCAGAACCTGCTGTACGATGTGTCACAGGTGGCGATCCCGTTTGATAACGTCGACGACGAGCAAATTCAAAAGCCGCAGCGTTGGAATCCGGCGGATCTGGGGCGCTTTATGATCTTCTTCGGACCGATCAGCTCGATCTTCGATAtttt                                                >  1:290391/1‑150 (MQ=255)
                                                                                                        aGAACCTGCTGTACGATGTGTCACAGGTGGCGATCCCGTTTGATAACGTCGACGACGAGCAAATTCAAAAGCCGCAGCGTTGGAATCCGGCGGATCTGGGGCGCTTTATGATCTTCTTCGGACCGATCAGCTCGATCTTCGATATTTTGa                                              >  1:2254417/1‑150 (MQ=255)
                                                                                                          aaCCTGCTGTACGATGTGTCACAGGTGGCGATCCCGTTTGATTACGTCGACGACGAGCAAATTCAAAAGCCGCAGCGTTGGAATCCGGCGGATCTGGGGCGCTTTATGATCTTCTTCGGACCGATCAGCTCGATCTTCGATATTTTGACg                                            <  1:154288/150‑1 (MQ=255)
                                                                                                          aaCCTGCTGTACGATGTGTCACAGGTGGCGATCCCGTTTGATTACGTCGACGACGAGCAAATTCAAAAGCCGCAGCGTTGGAATCCGGCGGATCTGGGGCGCTTTATGATCTTCTTCGGACCGATCAGCTCGATCTTCGATATTTTGACg                                            <  1:153431/150‑1 (MQ=255)
                                                                                                          aaCCTGCTGTACGATGTGTCACAGGTGGCGATCCCGTTTGATAACGTCGACGACGAGCAAATTCAAAAGCCGCAGCGTTGGAATCCGGCGGATCTGGGGCGCTTTATGATCTTCTTCGGACCGATCAGCTCGATCTTCGATATTTTGACg                                            >  2:1610576/1‑150 (MQ=255)
                                                                                                            ccTGCTGTACGATGTGTCACAGGTGGCGATCCCGTTTGATAACGTCGACGACGAGCAAATTCAAAAGCCGCAGCGTTGGAATCCGGCGGATCTGGGGCGCTTTATGATCTTCTTCGGACCGATCAGCTCGATCTTCGATATTTTGACGtt                                          <  2:1340915/150‑1 (MQ=255)
                                                                                                              tgctgTACGATGTGTCACAGGTGGCGATCCCGTTTGATAACGTCGACGACGAGCAAATTCAAAAGCCGCAGCGTTGGAATCCGGCGGACCTGGGGCGCTTTATGATCTTCTTCGGACCGATCAGCTCGATCTTCGATATTTTGACGtttt                                        >  1:3267405/1‑150 (MQ=255)
                                                                                                                 tgTACGATGTGTCACAGGTGGCGATCCCGTTTGATAACGTCGACGACGAGCAAATTCAAAAGCCGCAGCGTTGGAATCCGGCGGATCTGGGGCGCTTTATGATCTTCTTCGGACCGATCAGCTCGATCTTCGATATTTTGACGTTTTGcc                                     >  2:1930167/1‑150 (MQ=255)
                                                                                                                       aTGTGTCACAGGTGGCGATCCCGTTTGATTACGTCGACGACGAGCAAATTCAAAAGCCGCAGCGTTGGAATCCGGCGGATCTGGGGCGCTTTATGATCTTCTTCGGACCGATCAGCTCGATCTTCGATATTTTGACGTTTTGCCTGAtgt                               >  2:2221744/1‑150 (MQ=255)
                                                                                                                        tgtgTCACAGGTGGCGATCCCGTTTGATAACGTCGACGACGAGCAAATTCAAAAGCCGCAGCGTTGGAATCCGGCGGATCTGGGGCGCTTTATGATCTTCTTCGGACCGATCAGCTCGATCTTCGATCTTTTGACGTTTTGCCTGAtgtg                              >  2:4148308/1‑150 (MQ=255)
                                                                                                                        tgtgTCACAGGTGGCGATCCCGTTTGATAACGTCGACGACGAGCAAATTCAAAAGCCGCAGCGTTGGAATCCGGCGGATCTGGGGCGCTTTATGATCTTCTTCGGACCGATCAGCTCGATCTTCGATATTTTGACGTTTTGCCTGAtgtg                              <  1:4237268/150‑1 (MQ=255)
                                                                                                                           gtCACAGGTGGCGATCCCGTTTGATAACGTCGACGACGAGCAAATTCAAAAGCCGCAGCGTTGGAATCCGGCGGATCTGGGGCGCTTTATGATCTTCTTCGGACCGATCAGCTCGATCTTCGATATTTTGACGTTTTGCCTGATgtggtg                           >  2:331203/1‑150 (MQ=255)
                                                                                                                                  gTGGCGATCCCGTTTGATAACGTCGACGACGAGCAAATTCAAAAGCCGCAGCGTTGGAATCCGGCGGATCTGGGGCGCTTTATGATCTTCTTCGGACCGATCAGCTCGATCTTCGATATTTTGACGTTTTGCCTGATGTGGTGGGTATTc                    <  1:4127028/150‑1 (MQ=255)
                                                                                                                                      cGATCCCGTTTGATAACGTCGACGACGAGCAAATTCAAAAGCCGCAGCGTTGGAATCCGGCGGATCTGGGGCGCTTTATGATCTTCTTCGGACCGATCAGCTCGATCTTCGATATTTTGACGTTTTGCCTGATGTGGTGGGTATTCCATg                <  1:3315428/150‑1 (MQ=255)
                                                                                                                                      cGATCCCGTTTGATAACGTCGACGACGAGCAAATTCAAAAGCCGCAGCGTTGGAATCCGGCGGATCTGGGGCGCTTTATGATCTTCTTCGGACCGATCAGCTCGATCTTCGATATTTTGACGTTTTGCCTGATGTGGTGGGTATTCCATg                >  2:4455664/1‑150 (MQ=255)
                                                                                                                                      cGATCCCGTTTGAGAACGTCGACGACGAGCAAATTCAAAAGCCGCAGCGTTGGAATCCGGCGGATCTGGGGCGCTTTATGATCTTCTTCGGACCGATCAGCTCGATCTTCGATATTTTGACGTTTTGCCTGATGTGGTGGGTATTCCATg                <  1:853178/150‑1 (MQ=255)
                                                                                                                                       gATCCCGTTTGATAACGTCGACGACGAGCAAATTCAAAAGCCGCAGCGTTGGAATCCGGCGGATCTGGGGCGCTTTATGATCTTCTTCGGACCGATCAGCTCGATCTTCGATATTTTGACGTTTTGCCTGATGTGGTGGGTATTCCATGc               <  2:401930/150‑1 (MQ=255)
                                                                                                                                            cGTTTGATAACGTCGACGACGAGCAAATTCAAAAGCCGCAGCGTTGGAATCCGGCGGATCTGGGGCGCTTTATGATCTTCTTCGGACCGATCAGCTCGATCTTCGATATTTTGACGTTTTGCCTGATGTGGTGGGTATTCCATGCCAaca          <  1:2188659/150‑1 (MQ=255)
                                                                                                                                                tGATAACGTCGACGACGAGCAAATTCAAAAGCCGCAGCGTTGGAATCCGGCGGATCTGGGGCGCTTTATGATCTTCTTCGGACCGATCAGCTCGATCTTCGATATTTTGACGTTTTGCCTGATGTGGTGGGTATTCCATGCCAACACGcc      <  1:1152166/150‑1 (MQ=255)
                                                                                                                                                  aTAACGTCGACGACGAGCAAATTCAAAAGCCGCAGCGTTGGAATCCGGCGGATCTGGGGCGCTTTATGATCTTCTTCGGACCGATCAGCTCGATCTTCGATATTTTGACGTTTTGCCTGATGTGGTGGGTATTCCATGCCAACACGCCgg    <  1:2846886/150‑1 (MQ=255)
                                                                                                                                                  aTAACGTCGACGACGAGCAAATTCAAAAGCCGCAGCGTTGGAATCCGGCGGATCTGGGGCGCTTTATGATCTTCTTCGGACCGATCAGCTCGATCTTCGATATTTTGACGTTTTGCCTGATGTGGTGGGTATTACATGCCAACACGCCgg    >  1:521281/1‑150 (MQ=255)
                                                                                                                                                    |                                                                                                                                                     
CATCAAAATGACGGCGAGCTCTAACTTCGGTAATGTGTTCAGCGTGCTGGTAGCGAGTGCTTTCTTGCCCTTCCTGCCGATGTTGCCGTTACACTTGCTTATTCAGAACCTGCTGTACGATGTGTCACAGGTGGCGATCCCGTTTGATAACGTCGACGACGAGCAAATTCAAAAGCCGCAGCGTTGGAATCCGGCGGATCTGGGGCGCTTTATGATCTTCTTCGGACCGATCAGCTCGATCTTCGATATTTTGACGTTTTGCCTGATGTGGTGGGTATTCCATGCCAACACGCCGGAA  >  CP009273/4459535‑4459832

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑