BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	4,462,966	A→T	18.3%	Y21N (TAT→AAT)	yjgH ←	UPF0131 family protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	4,462,966	0	A	T	18.3%	665.8 / 139.0	250	Y21N (TAT→AAT)	yjgH	UPF0131 family protein
Reads supporting (aligned to +/- strand): ref base A (91/113); new base T (23/23); total (114/136)
Fisher's exact test for biased strand distribution p-value = 5.17e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.76e-01

Only 100 of 255 total aligned reads displayed.

AGTGCATCCCGCAGCTGCCAGGGTCGCATGCAAATTATCAAATGCCAGTCTGACTTGTTGCTGAAAATCGGGTTCTGGTGTTCCGTCCTCTCGACTTCCTACTTGCCCGGAAACAAACAGCAAATCGCCGGAACGAATAGCCGCAGAATAACGATGCTCAGCATATAGTGAATGTCGGCCAGCAGGGAAAACAGCGGTTCTTTCTACCATTTGGTTATCCTCAAGATTTACGACATGAACAGAAGATTTCTCTTTACCGGGAGCCGCTTTTAGCGGACGACGTGAGTAAACAAAACCCA  >  CP009273/4462817‑4463115

aGTGCATCCCGCAGCTGCCAGGGTCGCATGCAAATTATCAAATGCCAGTCTGACTTGTTGCTGAAAATCGGGTTCTGGTGTTCCGTCCTCTCGACTTCCTACTTGCCCGGAAACAAACAGCAAATCGCCGGAACGAATAGCCGCAGAATa                                                                                                                                                       <  1:1007330/150‑1 (MQ=255)

   gCATCCCGCAGCTGCCAGGGTCGCATGCAAATTATCAAATGCCAGTCTGACTTGTTGCTGAAAATCGGGTTCTGGTGTTCCGTCCTCTCGACTTCCTACTTGCCCGGAAACAAACAGCAAATCGCCGGAACGAATAGCCGCAGAATAACg                                                                                                                                                    >  1:2600110/1‑150 (MQ=255)

     aTCCCGCAGCTGCCAGGGTCGCATGCAAATTATCAAATGCCAGTCTGACTTGTTGCTGAAAATCGGGTTCTGGTGTTCCGTCCTCTCGACTTCCTACTTGCCCGGAAACAAACAGCAAATCGCCGGAACGAATAGCCGCAGAATAACGAt                                                                                                                                                  >  2:1781679/1‑150 (MQ=255)

       cccGCAGCTGCCAGGGTCGCATGCAAATTATCAAATGCCAGTCTGACTTGTTGCTGAAAATCGGGTTCTGGTGTTCCGTCCTCTCGACTTCCTACTTGCCCGGAAACAAACAGCAAATCGCCGGAACGAATAGCCGCAGAATAACGATGc                                                                                                                                                <  2:498634/150‑1 (MQ=255)

        ccGCAGCTGCCAGGGTCGCATGCAAATTATCAAATGCCAGTCTGACTTGTTGCTGAAAATCGGGTTCTGGTGTTCCGTCCTCTCGACTTCCTACTTGCCCGGAAACAAACAGCAAATCGCCGGAACGAATAGCCGCAGAATAACGATGCt                                                                                                                                               <  1:2431868/150‑1 (MQ=255)

         cGCAGCTGCCAGGGTCGCATGCAAATTATCAAATGCCAGTCTGACTTGTTGCTGAAAATCGGGTTCTGGTGTTCCGTCCTCTCGACTTCCTACTTGCCCGGAAACAAACAGCAAATCGCCGGAACGAATAGCCGCAGAATAACGATGCTc                                                                                                                                              <  2:3674943/150‑1 (MQ=255)

            aGCTGCCAGGGTCGCATGCAAATTATCAAATGCCAGTCTGACTTGTTGCTGAAAATCGGGTTCTGGTGTTCCGTCCTCTCGACTTCCTACTTGCCCGGAAACAAACAGCAAATCGCCGGAACGAATAGCCGCAGAATAACGATGCTCAGc                                                                                                                                           >  2:3552993/1‑150 (MQ=255)

              cTGCCAGGGTCGCATGCAAATTATCAAATGCCAGTCTGACTTGTTGCTGAAAATCGGGTTCTGGTGTTCCGTCCTCTCGACTTCCTACTTGCCCGGAAACAAACAGCAAATCGCCGGAACGAATAGCCGCAGAATTACGATGCTCAGCat                                                                                                                                         <  1:598472/150‑1 (MQ=255)

              cTGCCAGGGTCGCATGCAAATTATCAAATGCCAGTCTGACTTGTTGCTGAAAATCGGGTTCTGGTGTTCCGTCCTCTCGACTTCCTACTTGCCCGGAAACAAACAGCAAATCGCCGGAACGAATAGCCGCAGAATAACGATGCTCAGCag                                                                                                                                         >  1:2669707/1‑149 (MQ=255)

               tGCCAGGGTCGCATGCAAATTATCAAATGCCAGTCTGACTTGTTGCTGAAAATCGGGTTCTGGTGTTCCGTCCTCTCGACTTCCTACTTGCCCGGAAACAAACAGCAAATCGCCGGAACGAATAGCCGCAGAATTACGATGCTCAGCata                                                                                                                                        >  1:3726062/1‑150 (MQ=255)

                 ccAGGGTCGCATGCAAATTATCAAATGCCAGTCTGACTTGTTGCTGAAAATCGGGTTCTGGTGTTCCGTCCTCTCGACTTCCTACTTGCCCGGAAACAAACAGCAAATCGCCGGAACGAATAGCCGCAGAATTACGATGCTCAGCAtata                                                                                                                                      >  1:1588546/1‑150 (MQ=255)

                 ccAGGGTCGCATGCAAATTATCAAATGCCAGTCTGACTTGTTGCTGAAAATCGGGTTCTGGTGTTCCGTCCTCTCGACTTCCTACTTGCCCGGAAACAAACAGCAAATCGCCGGAACGAATAGCCGCAGAATAACGATGCTCAGCAtata                                                                                                                                      >  2:1527690/1‑150 (MQ=255)

                 ccAGGGTCGCATGCAAATTATCAAATGCCAGTCTGACTTGTTGCTGAAAATCGGGTTCTGGTGTTCCGTCCTCTCGACTTCCTACTTGCCCGGAAACAAACAGCAAATCGCCGGAACGAATAGCCGCAGAATAACGATGCTCAGCAtata                                                                                                                                      >  1:740052/1‑150 (MQ=255)

                  cAGGGTCGCATGCAAATTATCAAATGCCAGTCTGACTTGTTGCTGAAAATCGGGTTCTGGTGTTCCGTCCTCTCGACTTCCTACTTGCCCGGAAACAAACAGCAAATCGCCGGAACGAATAGCCGCAGAATAACGATGCTCAGCATATAg                                                                                                                                     >  2:134042/1‑150 (MQ=255)

                  cAGGGTCGCATGCAAATTATCAAATGCCAGTCTGACTTGTTGCTGAAAATCGGGTTCTGGTGTTCCGTCCTCTCGACTTCCTACTTGCCCGGAAACAAACAGCAAATCGCCGGAACGAATAGCCGCAGAATAACGATGCTCAGCATATAg                                                                                                                                     <  2:4180198/150‑1 (MQ=255)

                    gggTCGCATGCAAATTATCAAATGCCAGTCTGACTTGTTGCTGAAAATCGGGTTCTGGTGTTCCGTCCTCTCGACTTCCTACTTGCCCGGAAACAAACAGCAAATCGCCGGAACGAATAGCCGCAGAATAACGATGCTCAGCATATAGTg                                                                                                                                   >  1:1370890/1‑150 (MQ=255)

                     ggTCGCATGCAAATTATCAAATGCCAGTCTGACTTGTTGCTGAAAATCGGGTTCTGGTGTTCCGTCCTCTCGACTTCCTACTTGCCCGGAAACAAACAGCAAATCGCCGGAACGAATAGCCGCAGAATAACGATGCTCAGCATATAGTGa                                                                                                                                  <  1:2488320/150‑1 (MQ=255)

                       tCGCATGCAAATTATCAAATGCCAGTCTGACTTGTTGCTGAAAATCGGGTTCTGGTGTTCCGTCCTCTCGACTTCCTACTTGCCCGGAAACAAACAGCAAATCGCCGGAACGAATAGCCGCAGAATTACGATGCTCAGCATATAGTGAAt                                                                                                                                >  2:1410020/1‑150 (MQ=255)

                       tCGCATGCAAATTATCAAATGCCAGTCTGACTTGTTGCTGAAAATCGGGTTCTGGTGTTCCGTCCTCTCGACTTCCTACTTGCCCGGAAACAAACAGCAAATCGCCGGAACGAATAGCCGCAGAATTACGATGCTCAGCATATAGTGAAt                                                                                                                                <  1:1669157/150‑1 (MQ=255)

                       tCGCATGCAAATTATCAAATGCCAGTCTGACTTGTTGCTGAAAATCGGGTTCTGGTGTTCCGTCCTCTCGACTTCCTACTTGCCCGGAAACAAACAGCAAATCGCCGGAACGAATAGCCGCAGAATAACGATGCTCAGCATATAGTGAAt                                                                                                                                <  2:1882437/150‑1 (MQ=255)

                         gCATGCAAATTATCAAATGCCAGTCTGACTTGTTGCTGAAAATCGGGTTCTGGTGTTCCGTCCTCTCGACTTCCTACTTGCCCGGAAACAAACAGCAAATCGCCGGAACGAATAGCCGCAGAATTACGATGCTCAGCATATAGTGAATGt                                                                                                                              >  1:1942337/1‑150 (MQ=255)

                            tGCAAATTATCAAATGCCAGTCTGACTTGTTGCTGAAAATCGGGTTCTGGTGTTCCGTCCTCTCGACTTCCTACTTGCCCGGAAACAAACAGCAAATCGCCGGAACGAATAGCCGCAGAATAACGATGCTCAGCATATAGTGAATGTCgg                                                                                                                           >  1:4324754/1‑150 (MQ=255)

                             gCAAATTATCAAATGCCAGTCTGACTTGTTGCTGAAAATCGGGTTCTGGTGTTCCGTCCTCTCGACTTCCTACTTGCCCGGAAACAAACAGCAAATCGCCGGAACGAATAGCCGCAGAATAACGATGCTCAGCATATAGTGAATGTCGGc                                                                                                                          >  1:1761991/1‑150 (MQ=255)

                               aaaTTATCAAATGCCAGTCTGACTTGTTGCTGAAAATCGGGTTCTGGTGTTCCGTCCTCTCGACTTCCTACTTGCCCGGAAACAAACAGCAAATCGCCGGAACGAATAGCCGCAGAATAACGATGCTCAGCATATAGTGAATGTCGGCca                                                                                                                        >  1:1191591/1‑150 (MQ=255)

                                 aTTATCAAATGCCAGTCTGACTTGTTGCTGAAAATCGGGTTCTGGTGTTCCGTCCTCTCGACTTCCTACTTGCCCGGAAACAAACAGCAAATCGCCGGAACGAATAGCCGCAGAATAACGATGCTCAGCATATAGTGAATGTCGGCcagc                                                                                                                      >  1:3687098/1‑150 (MQ=255)

                                 aTTATCAAATGCCAGTCTGACTTGTTGCTGAAAATCGGGTTCTGGTGTTCCGTCCTCTCGACTTCCTACTTGCCCGGAAACAAACAGCAAATCGCCGGAACGAATAGCCGCAGAATAACGATGCTCAGCATATAGTGAATGTCGGCcagc                                                                                                                      >  2:1100451/1‑150 (MQ=255)

                                          tGCCAGTCTGACTTGTTGCTGAAAATCGGGTTCTGGTGTTCCGTCCTCTCGACTTCCTACTTGCCCGGAAACAAACAGCAAATCGCCGGAACGAATAGCCGCAGAATAACGATGCTCAGCATATAGTGAATGTCGGCCAGCAGGGAAAAc                                                                                                             <  1:2814389/150‑1 (MQ=255)

                                               gTCTGACTTGTTGCTGAAAATCGGGTTCTGGTGTTCCGTCCTCTCGACTTCCTACTTGCCCGGAAACAAACAGCAAATCGCCGGAACGAATAGCCGCAGAATTACGATGCTCAGCATATAGTGAATGTCGGCCAGCAGGGAAAACAGCgg                                                                                                        >  2:1291510/1‑150 (MQ=255)

                                               gTCTGACTTGTTGCTGAAAATCGGGTTCTGGTGTTCCGTCCTCTCGACTTCCTACTTGCCCGGAAACAAACAGCAAATCGCCGGAACGAATAGCCGCAGAATAACGATGCTCAGCATATAGTGAATGTCGGCCAGCAGGGAAAACAGCgg                                                                                                        >  2:3564878/1‑150 (MQ=255)

                                                 cTGACTTGTTGCTGAAAATCGGGTTCTGGTGTTCCGTCCTCTCGACTTCCTACTTGCCCGGAAACAAACAGCAAATCGCCGGAACGAATAGCCGCAGAATAACGATGCTCAGCATATAGTGAATGTCGGCCAGCAGGGAAAACAGCGGtt                                                                                                      <  1:2166108/150‑1 (MQ=255)

                                                   gACTTGTTGCTTAAAATCGGGTTCTGGTGTTCCGTCCTCTCGAATTCCTAATTGCCCGTAAACAAACAGCAAATCGCCGGACCGAATAGCGGCAGAATAACGATGCTCAGCATATAGTGAATGTCGGCCAGCTGGGAAAACAGCGGttct                                                                                                    <  1:623464/150‑1 (MQ=255)

                                                     cTTGTTGCTGAAAATCGGGTTCTGGTGTTCCGTCCTCTCGACTTCCTACTTGCCCGGAAACAAACAGCAAATCGCCGGAACGAATAGCCGCAGAATAACGATGCTCAGCATATAGTGAATGTCGGCCAGCAGGGAAAACAGCGGttcttt                                                                                                  >  2:1688397/1‑150 (MQ=255)

                                                       tgttgCTGAAAATCGGGTTCTGGTGTTCCGTCCTCTCGACTTCCTACTTGCCCGGAAACAAACAGCAAATCGCCGGAACGAATAGCCGCAGAATTACGATGCTCAGCATATAGTGAATGTCGGCCAGCAGGGAAAACAGCGGttctttct                                                                                                <  2:2989539/150‑1 (MQ=255)

                                                        gttgCTGAAAATCGGGTTCTGGTGTTCCGTCCTCTCGACTTCCTACTTGCCCGGAAACAAACAGCAAATCGCCGGAACGAATAGCCGCAGAATAACGATGCTCAGCATATAGTGAATGTCGGCCAGCAGGGAAAACAGCGGTTCTTTCTa                                                                                               >  1:1047783/1‑150 (MQ=255)

                                                         ttgCTGAAAATCGGGTTCTGGTGTTCCGTCCTCTCGACTTCCTACTTGCCCGGAAACAAACAGCAAATCGCCGGAACGAATAGCCGCAGAATAACGATGCTCAGCATATAGTGAATGTCGGCCAGCAGGGAAAACAGCGGTTCTTTCTAc                                                                                              >  2:4335911/1‑150 (MQ=255)

                                                         ttgCTGAAAATCGGGTTCTGGTGTTCCGTCCTCTCGACTTCCTACTTGCCCGGAAACAAACAGCAAATCGCCGGAACGAATAGCCGCAGAATAACGATGCTCAGCATATAGTGAATGTCGGCCAGCAGGGAAAACAGCGGTTCTTTCTAc                                                                                              >  2:4335947/1‑150 (MQ=255)

                                                           gCTGAAAATCGGGTTCTGGTGTTCCGTCCTCTCGACTTCCTACTTGCCCGGAAACAAACAGCAAATCGCCGGAACGAATAGCCGCAGAATTACGATGCTCAGCATATAGTGAATGTCGGCCAGCAGGGAAAACAGCGGTTCTTTCTACCa                                                                                            >  2:4034084/1‑150 (MQ=255)

                                                           gCTGAAAATCGGGTTCTGGTGTTCCGTCCTCTCGACTTCCTACTTGCCCGGAAACAAACAGCAAATCGCCGGAACGAATAGCCGCAGAATAACGATGCTCAGCATATAGTGAATGTCGGCCAGCAGGGAAAACAGCGGTTCTTTCTACCa                                                                                            <  1:1980911/150‑1 (MQ=255)

                                                           gCTGAAAATCGGGTTCTGGTGTTCCGTCCTCTCGACTTCCTACTTGCCCGGAAACAAACAGCAAATCGCCGGAACGAATAGCCGCAGAATAACGATGCTCAGCATATAGTGAATGTCGGCCAGCAGGGAAAACAGCGGTTCTTTCTACCa                                                                                            <  2:1958952/150‑1 (MQ=255)

                                                                    cGGGTTCTGGTGTTCCGTCCTCTCGACTTCCTACTTGCCCGGAAACAAACAGCAAATCGCCGGAACGAATAGCCGCAGAATAACGATGCTCAGCATATAGTGAATGTCGGCCAGCAGGGAAAACAGCGGTTCTTTCTACCATTTGGTTAt                                                                                   <  2:587838/150‑1 (MQ=255)

                                                                    cGGGTTCTGGTGTTCCGTCCTCTCGACTTCCTACTTGCCCGGAAACAAACAGCAAATCGCCGGAACGAATAGCCGCAGAATAACGATGCTCAGCATATAGTGAATGTCGGCCAGCAGGGAAAACAGCGGTTCTTTCTACCATTTGGTTAt                                                                                   <  2:4405218/150‑1 (MQ=255)

                                                                    cGGGTTCTGGTGTTCCGTCCTCTCGACTTCCTACTTGCCCGGAAACAAACAGCAAATCGCCGGAACGAATAGCCGCAGAATAACGATGCTCAGCATATAGTGAATGTCGGCCAGCAGGGAAAACAGCGGTTCTTTCTACCATTTGGTTAt                                                                                   >  2:1200509/1‑150 (MQ=255)

                                                                      ggTTCTGGTGTTCCGTCCTCTCGACTTCCTACTTGCCCGGAAACAAACAGCAAATCGCCGGAACGAATAGCCGCAGAATAACGATGCTCAGCATATAGTGAATGTCGGCCAGCAGGGAAAACAGCGGTTCTTTCTACCATTTGGTTATcc                                                                                 >  2:3747311/1‑150 (MQ=255)

                                                                          cTGGTGTTCCGTCCTCTCGACTTCCTACTTGCCCGGAAACAAACAGCAAATCGCCGGAACGAATAGCCGCAGAATTACGATGCTCAGCATATAGTGAATGTCGGCCAGCAGGGAAAACAGCGGTTCTTTCTACCATTTGGTTATCCTCaa                                                                             >  2:1004410/1‑150 (MQ=255)

                                                                            ggTGTTCCGTCCTCTCGACTTCCTACTTGCCCGGAAACAAACAGCAAATCGCCGGAACGAATAGCCGCAGAATAACGATGCTCAGCATATAGTGAATGTCGGCCAGCAGGGAAAACAGCGGTTCTTTCTACCATTTGGTTATCCTCAAGa                                                                           <  1:809159/150‑1 (MQ=255)

                                                                                ttCCGTCCTCTCGACTTCCTACTTGCCCGGAAACAAACAGCAAATCGCCGGAACGAATAGCCGCAGAATAACGATGCTCAGCATATAGTGAATGTCGGCCAGCAGGGAAAACAGCGGTTCTTTCTACCATTTGGTTATCCTCAAGATTTa                                                                       >  1:3862690/1‑150 (MQ=255)

                                                                                ttCCGTCATCTCGACTTCATACTTGCCCGGAAACAAACAGCAAATCGCCGTAAGTAATAGCCGCAGAATAACGATGCTCATCATATAGTGTAGGTCGGCCAGAAGGGAAAACAGCGGTTCTTTCTACCATTTGGTTATCCTCAAGATTTa                                                                       <  1:4176136/150‑1 (MQ=255)

                                                                                 tCCGTCCTCTCGACTTCCTACTTGCCCGGAAACAAACAGCAAATCGCCGGAACGAATAGCCGCAGAATAACGATGCTCAGCATATAGTGAATGTCGGCCAGCAGGGACAACAGCGGTTCTTTCTACCATTTGGTTATCCTCAAGATTTAc                                                                      >  1:2517712/1‑150 (MQ=255)

                                                                                 tCCGTCCTCTCGACTTCCTACTTGCCCGGAAACAAACAGCAAATCGCCGGAACGAATAGCCGCAGAATAACGATGCTCAGCATATAGTGAATGTCGGCCAGCAGGGAAAACAGCGGTTCTTTCTACCATTTGGTTATCCTCAAGATTTAc                                                                      <  2:2776306/150‑1 (MQ=255)

                                                                                 tCCGTCCTCTCGACTTCCTACTTGCCCGGAAACAAACAGCAAATCGCCGGAACGAATAGCCGCAGAATAACGATGCTCAGCATATAGTGAATGTCGGCCAGCAGGGAAAACAGCGGTTCTTTCTACCATTTGGTTATCCTCAAGATTTAc                                                                      <  1:4525188/150‑1 (MQ=255)

                                                                                   cGTCCTCTCGACTTCCTACTTGCCCGGAAACAAACAGCAAATCGCCGGAACGAATAGCCGCAGAATTACGATGCTCAGCATATAGTGAATGTCGGCCAGCAGGGAAAACAGCGGTTCTTTCTACCATTTGGTTATCCTCAAGATTTACGa                                                                    >  2:3332267/1‑150 (MQ=255)

                                                                                    gTCCTCTCGACTTCCTACTTGCCCGGAAACAAACAGCAAATCGCCGGAACGAATAGCCGCAGAATAACGATGCTCAGCATATAGTGAATGTCGGCCAGCAGGGAAAACAGCGGTTCTTTCTACCATGTGGTTATCCTCAAGATTTACGAc                                                                   <  2:594625/150‑1 (MQ=255)

                                                                                      ccTCTCGACTTCCTACTTGCCCGGAAACAAACAGCAAATCGCCGGAACGAATAGCCGCAGAATAACGATGCTCAGCATATAGTGAATGTCGGCCAGCAGGGAAAACAGCGGTTCTTTCTACCATTTGGTTATCCTCAAGATTTACGACAt                                                                 >  1:454645/1‑150 (MQ=255)

                                                                                      ccTCTCGACTTCCTACTTGCCCGGAAACAAACAGCAAATCGCCGGAACGAATAGCCGCAGAATAACGATGCTCAGCATATAGTGAATGTCGGCCAGCAGGGAAAACAGCGGTTCTTTCTACCATTTGGTTATCCTCAAGATTTACGACAt                                                                 <  1:2356400/150‑1 (MQ=255)

                                                                                      ccTCTCGACTTCCCACTTGCCCGGAAACAAACAGCAAATCGCCGGAACGAATAGCCGCAGAATAACGATGCTCAGCATATAGTGAATGTCGGCCAGCAGGGAAAACAGCGGTTCTTTCTACCATTTGGTTATCCTCAAGATTTACGACAt                                                                 <  1:3590254/150‑1 (MQ=255)

                                                                                        tctcGACTTCCTACTTGCCCGGAAACAAACAGCAAATCGCCGGAACGAATAGCCGCAGAATTACGATGCTCAGCATATAGTGAATGTCGGCCAGCAGGGAAAACAGCGGTTCTTTCTACCATTTGGTTATCCTCAAGATTTACGACATGa                                                               <  1:2037121/150‑1 (MQ=255)

                                                                                        tctcGACTTCCTACTTGCCCGGAAACAAACAGCAAATCGCCGGAACGAATAGCCGCAGAATAACGATGCTCAGCATATAGTGAATGTCGGCCAGCAGGGAAAACAGCGGTTCTTTCTACCATTTGGTTATCCTCAAGATTTACGACATGa                                                               >  2:1612730/1‑150 (MQ=255)

                                                                                        tctcGACTTCCTACTTGCCCGGAAACAAACAGCAAATCGCCGGAACGAATAGCCGCAGAATAACGATGCTCAGCATATAGTGAATGTCGGCCAGCAGGGAAAACAGCGGTTCTTTCTACCATTTGGTTATCCTCAAGATTTACGACATGa                                                               <  1:3378908/150‑1 (MQ=255)

                                                                                        tctcGACTTCCTACTTGCCCGGAAACAAACAGCAAATCGCCGGAACGAATAGCCGCAGAATAACGATGCTCAGCATATAGTGAATGTCGGCCAGCAGGGAAAACAGCGGTTCTTTCTACCATTTGGTTATCCTCAAGATTTACGACATGa                                                               <  2:4471971/150‑1 (MQ=255)

                                                                                           cGACTTCCTACTTGCCCGGAAACAAACAGCAAATCGCCGGAACGAATAGCCGCAGAATTACGATGCTCAGCATATAGTGAATGTCGGCCAGCAGGGAAAACAGCGGTTCTTTCTACCATTTGGTTATCCTCAAGATTTACGACATGAACa                                                            <  1:2974686/150‑1 (MQ=255)

                                                                                           cGACTTCCTACTTGCCCGGAAACAAACAGCAAATCGCCGGAACGAATAGCCGCAGAATTACGATGCTCAGCATATAGTGAATGTCGGCCAGCAGGGAAAACAGCGGTTCTTTCTACCATTTGGTTATCCTCAAGATTTACGACATGAACa                                                            <  1:2974891/150‑1 (MQ=255)

                                                                                              cTTCCTACTTGCCCGGAAACAAACAGCAAATCGCCGGAACGAATAGCCGCAGAATAACGATGCTCAGCATATAGTGAATGTCGGCCAGCAGGGAAAACAGCGGTTCTTTCTACCATTTGGTTATCCTCAAGATTTACGACATGAACagaa                                                         >  1:3029358/1‑150 (MQ=255)

                                                                                              cTTACTACTTGCCCGGAAACAAACAGCAAATCGCCGGAACGAATAGCCGCAGAATAACGATGCTCAGCATATAGTGAATGTCGGCCAGCAGGGAAAACAGCGGTTCTTTCTACCATTTGGTTATCCTCAAGATTTACGACATGAACagaa                                                         <  2:3827191/150‑1 (MQ=255)

                                                                                               ttCCTACTTGCCCGGAAACAAACAGCAAATCGCCGGAACGAATAGCCGCAGAATTACGATGCTCAGCATATAGTGAATGTCGGCCAGCAGGGAAAACAGCGGTTCTTTCTACCATTTGGTTATCCTCAAGATTTACGACATGAACagaag                                                        >  1:1311906/1‑150 (MQ=255)

                                                                                                  cTACTTGCCCGGAAACAAACAGCAAATCGCCGGAACGAATAGCCGCAGAATAACGATGCTCAGCATATAGTGAATGTCGGCCAGCAGGGAAAACAGCGGTTCTTTCTACCATTTGGTTATCCTCAAGATTTACGACATGAACAGAAGAtt                                                     <  1:2718038/150‑1 (MQ=255)

                                                                                                   tACTTGCCCGGAAACAAACAGCAAATCGCCGGAACGAATAGCCGCAGAATTACGATGCTCAGCATATAGTGAATGTCGGCCAGCAGGGAAAACAGCGGTTCTTTCTACCATTTGGTTATCCTCAAGATTTACGACATGAACAGAAGAttt                                                    >  2:312404/1‑150 (MQ=255)

                                                                                                    aCTTGCCCGGAAACAAACAGCAAATCGCCGGAACGAATAGCCGCAGAATAACGATGCTCAGCATATAGTGAATGTCGGCCAGCAGGGAAAACAGCGGTTCTTTCTACCATTTGGTTATCCTCAAGATTTACGACATGAACAGAAGATTtc                                                   >  1:3973259/1‑150 (MQ=255)

                                                                                                       tGCCCGGAAACAAACAGCAAATCGCCGGAACGAATAGCCGCAGAATAACGATGCTCAGCATATAGTGAATGTCGGCCAGCAGGGAAAACAGCGGTTCTTTCTACCATTTGGTTATCCTCAAGATTTACGACATGAACAGAAGATTTctct                                                <  1:2866862/150‑1 (MQ=255)

                                                                                                           cGGAAACAAACAGCAAATCGCCGGAACGAATAGCCGCAGAATTACGATGCTCAGCATATAGTGAATGTCGGCCAGCAGGGAAAACAGCGGTTCTTTCTACCATTTGGTTATCCTCAAGATTTACGACATGAACAGAAGATTTCTCTTTAc                                            >  2:3148004/1‑150 (MQ=255)

                                                                                                            ggAAACAAACAGCAAATCGCCGGAACGAATAGCCGCAGAATAACGATGCTCAGCATATAGTGAATGTCGGCCAGCAGGGAAAACAGCGGTTCTTTCTACCATTTGGTTATCCTCAAGATTTACGACATGAACAGAAGATTTCTCTTTAcc                                           <  2:778707/150‑1 (MQ=255)

                                                                                                            ggAAACAAACAGCAAATCGCCGGAACGAATAGCCGCAGAATAACGATGCTCAGCATATAGTGAATGTCGGCCAGCAGGGAAAACAGCGGTTCTTTCTACCATTTGGTTATCCTCAAGATTTACGACATGAACAGAAGATTTCTCTTTAcc                                           <  1:4443294/150‑1 (MQ=255)

                                                                                                             gAAACAAACAGCAAATCGCCGGAACGAATAGCCGCAGAATAACGATGCTCAGCATATAGTGAATGTCGGCCAGCAGGGAAAACAGCGGTTCTTTCTACCATTTGGTTATCCTCAAGATTTACGACATGAACAGAAGATTTCTCTTTACCg                                          <  1:755952/150‑1 (MQ=255)

                                                                                                              aaacaaacAGCAAATCGCCGGAACGAATAGCCGCAGAATTACGATGCTCAGCATATAGTGAATGTCGGCCAGCAGGGAAAACAGCGGTTCTTTCTACCATTTGGTTATCCTCAAGATTTACGACATGAACAGAAGATTTCTCTTTACCgg                                         >  2:3701088/1‑150 (MQ=255)

                                                                                                              aaacaaacAGCAAATCGCCGGAACGAATAGCCGCAGAATAACGATGCTCAGCATATAGTGAATGTCGGCCAGCAGGGAAAACAGCGGTTCTTTCTACCATTTGGTTATCCTCAAGATTTACGACATGAACAGAAGATTTCTCTTTACCgg                                         >  2:3139468/1‑150 (MQ=255)

                                                                                                              aaacaaacAGCAAATCGCCGGAACGAATAGCCGCAGAATAACGATGCTCAGCATATAGTGAATGTCGGCCAGCAGGGAAAACAGCGGTTCTTTCTACCATTTGGTTATCCTCAAGATTTACGACATGAACAGAAGATTTCTCTTTACCgg                                         >  2:2398407/1‑150 (MQ=255)

                                                                                                               aacaaacaGCAAATCGCCGGAACGAATAGCCGCAGAATAACGATGCTCAGCATATAGTGAATGTCGGCCAGCAGGGAAAACAGCGGTTCTTTCTACCATTTGGTTATCCTCAAGATTTACGACATGAACAGAAGATTTCTCTTTACCggg                                        <  2:2514613/150‑1 (MQ=255)

                                                                                                                acaaacaGCAAATCGCCGGAACGAATAGCCGCAGAATTACGATGCTCAGCATATAGTGAATGTCGGCCAGCAGGGAAAACAGCGGTTCTTTCTACCATTTGGTTATCCTCAAGATTTACGACATGAACAGAAGATTTCTCTTTACCGGGa                                       >  1:3325761/1‑150 (MQ=255)

                                                                                                                    acaGCAAATCGCCGGAACGAATAGCCGCAGAATAACGATGCTCAGCATATAGTGAATGTCGGCCAGCAGGGAAAACAGCGGTTCTTTCTACCATTTGGTTATCCTCAAGATTTACGACATGAACAGAAGATTTCTCTTTACCGGGAGCCg                                   <  1:3516271/150‑1 (MQ=255)

                                                                                                                      aGCAAATCGCCGGAACGAATAGCCGCAGAATAACGATGCTCAGCATATAGTGAATGTCGGCCAGCAGGGAAAACAGCGGTTCTTTCTACCATTTGGTTATCCTCAAGATTTACGACATGAACAGAAGATTTCTCTTTACCGGGAGCCGCt                                 >  2:843167/1‑150 (MQ=255)

                                                                                                                       gCAAATCGCCGGAACGAATAGCCGCAGAATTACGATGCTCAGCATATAGTGAATGTCGGCCAGCAGGGAAAACAGCGGTTCTTTCTACCATTTGGTTATCCTCAAGATTTACGACATGAACAGAAGATTTCTCTTTACCGGGAGCCGCtt                                <  1:117248/150‑1 (MQ=255)

                                                                                                                        cAAATCGCCGGAACGAATAGCCGCAGAATTACGATGCTCAGCATATAGTGAATGTCGGCCAGCAGGGAAAACAGCGGTTCTTTCTACCATTTGGTTATCCTCAAGATTTACGACATGAACAGAAGATTTCTCTTTACCGGGAGCCGCttt                               >  1:1010356/1‑150 (MQ=255)

                                                                                                                        cAAATCGCCGGAACGAATAGCCGCAGAATAACGATGCTCAGCATATAGTGAATGTCGGCCAGCAGGGAAAACAGCGGTTCTTTCTACCATTTGGTTATCCTCAAGATTTACGACATGAACAGAAGATTTCTCTTTACCGGGAGCCGCttt                               <  1:3671128/150‑1 (MQ=255)

                                                                                                                          aaTCGCCGGAACGAATAGCCGCAGAATAACGATGCTCAGCATATAGTGAATGTCGGCCAGCAGGGAAAACAGCGGTTCTTTCTACCATTTGGTTATCCTCAAGATTTACGACATGAACAGAAGATTTCTCTTTACCGGGAGCCGCTTTTa                             <  2:2369482/150‑1 (MQ=255)

                                                                                                                          aaTCGCCGGAACGAATAGCCGCAGAATAACGATGCTCAGCATATAGTGAATGTCGGCCAGCAGGGAAAACAGCGGTTCTTTCTACCATTTGGTTATCCTCAAGATTTACGACATGAACAGAAGATTTCTCTTTACCGGGAGCCGCTTTTa                             >  1:832113/1‑150 (MQ=255)

                                                                                                                          aaTCGCCGGAACGAATAGCCGCAGAATAACGATGCTCAGCATATAGTGAATGTCGGCCAGCAGGGAAAACAGCGGTTCTTTCTACCATTTGGTTATCCTCAAGATTTACGACATGAACAGAAGATTTCTCTTTACCGGGAGCCGCTTTTa                             >  1:832053/1‑150 (MQ=255)

                                                                                                                          aaTCGCCGGAACGAATAGCCGCAGAATAACGATGCTCAGCATATAGTGAATGTCGGCCAGCAGGGAAAACAGCGGTTCTTTCTACCATTTGGTTATCCTCAAGATTTACGACATGAACAGAAGATTTCTCTTTACCGGGAGCCGCTTTTa                             <  1:2660504/150‑1 (MQ=255)

                                                                                                                                 ggAACGAATAGCCGCAGAATTACGATGCTCAGCATATAGTGAATGTCGGCCAGCAGGGAAAACAGCGGTTCTTTCTACCATTTGGTTATCCTCAAGATTTACGACATGAACAGAAGATTTCTCTTTACCGGGAGCCGCTTTTAGCGgacg                      <  1:4484061/150‑1 (MQ=255)

                                                                                                                                   aaCGAATAGCCGCAGAATAACGATGCTCAGCATATAGTGAATGTCGGCCAGCAGGGAAAACAGCGGTTCTTTCTACCATTTGGTTATCCTCAAGATTTACGACATGAACAGAAGATTTCTCTTTACCGGGAGCCGCTTTTAGCGgacgac                    >  1:2934682/1‑150 (MQ=255)

                                                                                                                                    aCGAATAGCCGCAGAATAACGATGCTCAGCATATAGTGAATGTCGGCCAGCAGGGAAAACAGCGGTTCTTTCTACCATTTGGTTATCCTCAAGATTTACGACATGAACAGAAGATTTCTCTTTACCGGGAGCCGCTTTTAGCGGacgacg                   <  2:2094699/150‑1 (MQ=255)

                                                                                                                                       aaTAGCCGCAGAATAACGATGCTCAGCATATAGTGAATGTCGGCCAGCAGGGAAAACAGCGGTTCTTTCTACCATTTGGTTATCCTCAAGATTTACGACATGAACAGAAGATTTCTCTTTACCGGGAGCCGCTTTTAGCGGACGACGTGa                >  1:3181322/1‑150 (MQ=255)

                                                                                                                                        aTAGCCGCAGAATAACGATGCTCAGCATATAGTGAATGTCGGCCAGCAGGGAAAACAGCGGTTCTTTCTACCATTTGGTTATCCTCAAGATTTACGACATGAACAGAAGATTTCTCTTTACCGGGAGCCGCTTTTAGCGGACGACGTGAg               <  1:134042/150‑1 (MQ=255)

                                                                                                                                        aTAGCCGCAGAATAACGATGCTCAGCATATAGTGAATGTCGGCCAGCAGGGAAAACAGCGGTTCTTTCTACCATTTGGTTATCCTCAAGATTTACGACATGAACAGAAGATTTCTCTTTACCGGGAGCCGCTTTTAGCGGACGACGTGAg               <  2:4313320/150‑1 (MQ=255)

                                                                                                                                         tAGCCGCAGAATAACGATGCTCAGCATATAGTGAATGTCGGCCAGCAGGGAAAACAGCGGTTCTTTCTACCATTTGGTTATCCTCAAGATTTACGACATGAACAGAAGATTTCTCTTTACCGGGAGCCGCTTTTAGCGGACGACGTGAGt              >  2:2634419/1‑150 (MQ=255)

                                                                                                                                            ccGCAGAATAACGATGCTCAGCATATAGTGAATGTCGGCCAGCAGGGAAAACAGCGGTTCTTTCTACCATTTGGTTATCCTCAAGATTTTCGACATGAACAGAAGATTTCTCTTTACCGGGAGCCGCTTTTAGCGGACGACGTGAGTaaa           <  2:1474643/150‑1 (MQ=255)

                                                                                                                                              gCAGAATTACGATGCTCAGCATATAGTGAATGTCGGCCAGCAGGGAAAACAGCGGTTCTTTCTACCATTTGGTTATCCTCAAGATTTACGACATGAACAGAAGATTTCTCTTTACCGGGAGCCGCTTTTAGCGGACGACGTGAGTAAACa         >  2:3314765/1‑150 (MQ=255)

                                                                                                                                                aGAATAACGATGCTCAGCATATAGTGAATGTCGGCCAGCAGGGAAAACAGCGGTTCTTTCTACCATTTGGTTATCCTCAAGATTTACGACATGAACAGAAGATTTCTCTTTACCGGGAGCCGCTTTTAGCGGACGACGTGAGTAAACaaa       >  1:3599439/1‑150 (MQ=255)

                                                                                                                                                aGAATAACGATGCTCAGCATATAGTGAATGTCGGCCAGCAGGGAAAACAGCGGTTCTTTCTACCATTTGGTTATCCTCAAGATTTACGACATGAACAGAAGATTTCTCTTTACCGGGAGCCGCTTTTAGCGGACGACGTGAGTAAACaaa       >  2:1025048/1‑150 (MQ=255)

                                                                                                                                                  aaTAACGATGCTCAGCATATAGTGAATGTCGGCCAGCAGGGAAAACAGCGGTTCTTTCTACCATTTGGTTATCCTCAAGATTTACGACATGAACAGAAGATTTCTCTTTACCGGGAGCCGCTTTTAGCGGACGACGTGAGTAAACAAAAc     <  1:4364337/150‑1 (MQ=255)

                                                                                                                                                    tAACGATGCTCAGCATATAGTGAATGTCGGCCAGCAGGGAAAACAGCGGTTCTTTCTACCATTTGGTTATCCTCAAGATTTACGACATGAACAGAAGATTTCTCTTTACCGGGAGCCGCTTTTAGCGGACGACGTGAGTAAACAAAAccc   <  1:3370127/150‑1 (MQ=255)

                                                                                                                                                     aaCGATGCTCAGCATATAGTGAATGTCGGCCAGCAGGGAAAACAGCGGTTCTTTCTACCATTTGGTTATCCTCAAGATTTACGACATGAACAGAAGATTTCTCTTTACCGGGAGCCGCTTTTAGCGGACGACGTGAGTAAACAAAACCCa  >  2:1095976/1‑150 (MQ=255)

AGTGCATCCCGCAGCTGCCAGGGTCGCATGCAAATTATCAAATGCCAGTCTGACTTGTTGCTGAAAATCGGGTTCTGGTGTTCCGTCCTCTCGACTTCCTACTTGCCCGGAAACAAACAGCAAATCGCCGGAACGAATAGCCGCAGAATAACGATGCTCAGCATATAGTGAATGTCGGCCAGCAGGGAAAACAGCGGTTCTTTCTACCATTTGGTTATCCTCAAGATTTACGACATGAACAGAAGATTTCTCTTTACCGGGAGCCGCTTTTAGCGGACGACGTGAGTAAACAAAACCCA  >  CP009273/4462817‑4463115

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`