Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 4,616,297 C→A 5.7% S190* (TCG→TAG)  radA → DNA repair protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092734,616,2970CA 5.7% 961.9 / 25.6 252S190* (TCG→TAG) radADNA repair protein
Reads supporting (aligned to +/- strand):  ref base C (114/123);  new base A (13/2);  total (127/125)
Fisher's exact test for biased strand distribution p-value = 5.85e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 252 total aligned reads displayed.

GCTCATCGCCTTGGCCTGCCGACTGACAATCTCAATATGTTGTCGGAAACCAGCATCGAACAGATCTGCCTGATTGCCGAAGAAGAGCAACCGAAGCTGATGGTAATTGACTCGATCCAGGTGATGCATATGGCGGATGTACAGTCATCGCCTGGTAGCGTGGCGCAGGTGCGTGAAACGGCGGCTTATTTGACACGCTTCGCCAAAACGCGCGGTGTGGCGATTGTCATGGTGGGGCACGTAACCAAAGATGGTTCGCTGGCTGGCCCGAAAGTGCTGGAACACTGTATCGACTGT  >  CP009273/4616149‑4616445
                                                                                                                                                    |                                                                                                                                                    
gcTCATCGCCTTGGCCTGCCGACTGACAATCTCAATATGTTGTCGGAAACCAGCATCGAACAGATCTGCCTGATTGCCGAAGAAGAGCAACCGAAGCTGATGGTAATTGACTCGATCCAGGTGATGCATATGGCGGATGTACAGTCATCg                                                                                                                                                     >  1:2089666/1‑150 (MQ=255)
gcTCATCGCCTTGGCCTGCCGACTGACAATCTCAATATGTTGTCGGAAACCAGCATCGAACAGATCTGCCTGATTGCCGAAGAAGAGCAACCGAAGCTGATGGTAATTGACTCGATCCAGGTGATGCATATGGCGGATGTACAGTCATCg                                                                                                                                                     >  2:4120289/1‑150 (MQ=255)
  tCATCGCCTTGGCCTGCCGACTGACAATCTCAATATGTTGTCGGAAACCAGCATCGAACAGATCTGCCTGATTGCCGAAGAAGAGCAACCGAAGCTGATGGTAATTGACTCGATCCAGGTGATGCATATGGCGGATGTACAGTCATAGcc                                                                                                                                                   >  1:1308550/1‑150 (MQ=255)
   cATCGCCTTGGCCTGCCGACTGACAATCTCAATATGTTGTCGGAAACCAGCATCGAACAGATCTGCCTGATTGCCGAAGAAGAGCAACCGAAGCTGATGGTAATTGACTCGATCCAGGTGATGCATATGGCGGATGTACAGTCATCGCCt                                                                                                                                                  <  1:3215158/150‑1 (MQ=255)
   cATCGCCTTGGCCTGCCGACTGACAATCTCAATATGTTGTCGGAAACCAGCATCGAACAGATCTGCCTGATTGCCGAAGAAGAGCAACCGAAGCTGATGGTAATTGACTCGATCCAGGTGATGCATATGGCGGATGTACAGTCATCGCCt                                                                                                                                                  <  1:940099/150‑1 (MQ=255)
       gCCTTGGCCTGCCGACTGACAATCTCAATATGTTGTCGGAAACCAGCATCGAACAGATCTGCCTGATTGCGGAAGAAGAGCAACCGAAGCTGATGGTAATTGACTCGATCCAGGTGATGCATATGGCGGATGTACAGTCATCGCCTGGTa                                                                                                                                              >  2:2014143/1‑150 (MQ=255)
         cTTGGCCTGCCGACTGACACTCTCAATCTGTTGTCGGAAACCAGCATCGAACAGATCTGCCTGATTGCCGAAGAAGAGCAACCGAAGCTGATGGTAATTGACTCGATCCAGGTGATGCATATGGCGGATGTACAGTCATCGCCTGGTAGc                                                                                                                                            <  1:4432864/150‑1 (MQ=255)
               cTGCCGACTGACAATCTCAATATGTTGTCGGAAACCAGCATCGAACAGATCTGCCTGATTGCCGAAGAAGAGCAACCGAAGCTGATGGTAATTGACTCGATCCAGGTGATGCATATGGCGGATGTACAGTCATCGCCTGGTAGCGTGgcg                                                                                                                                      <  2:12740/150‑1 (MQ=255)
                 gCCGACTGACAATCTCAATATGTTGTCGGAAACCAGCATCGAACAGATCTGCCTGATTGCCGAAGAAGAGCAACCGAAGCTGATGGTAATTGACTCGATCCAGGTGATGCATATGGCGGATGTACAGTCATCGCCTGGTAGCGTGGCGCa                                                                                                                                    <  2:3918902/150‑1 (MQ=255)
                    gACTGACAATCTCCATATGTTGTCGGAAACCAGCATCGAACAGATCTGCCTGATTGCCGAAGAAGAGCAACCGAAGCTGATGGTAATTGACTCGATCCAGGTGATGCATATGGCGGATGTACAGTCATCGCCTGGTAGCGTGGCGCAGGt                                                                                                                                 <  1:1053355/150‑1 (MQ=255)
                     aCTGACAATCTCAATATGTTGTCGGAAACCAGCATCGAACAGATCTGCCTGATTGCCGAAGAAGAGCAACCGAAGCTGATGGTAATTGACTCGATCCAGGTGATGCATATGGCGGATGTACAGTCATCGCCTGGTAGCGTGGCGCAGGTg                                                                                                                                >  2:1714617/1‑150 (MQ=255)
                      cTGACAATCTCAATATGTTGTCGGAAACCAGCATCGAACAGATCTGCCTGATTGCCGAAGAAGAGCAACCGAAGCTGATGGTAATTGACTCGATCCAGGTGATGCATATGGCGGATGTACAGTCATAGCCTGGTAGCGTGGCGCAGGTGc                                                                                                                               >  2:3890987/1‑150 (MQ=255)
                       tGACAATCTCAATATGTTGTCGGAAACCAGCATCGAACAGATCTGCCTGATTGCCGAAGAAGAGCAACCGAAGCTGATGGTAATTGACTCGATCCAGGTGATGCATATGGCGGATGTACAGTCATCGCCTGGTAGCGTGGCGCAGGTGCg                                                                                                                              >  2:1689715/1‑150 (MQ=255)
                        gACAATCTCAATATGTTGTCGGAAACCAGCATCGAACAGATCTGCCTGATTGCCGAAGAAGAGCAACCGAAGCTGATGGTAATTGACTCGATCCAGGTGATGCATATGGCGGATGTACAGTCATCGCCTGGTAGCGTGGCGCAGGTGCGt                                                                                                                             >  1:4142111/1‑150 (MQ=255)
                        gACAATCTCAATATGTTGTCGGAAACCAGCATCGAACAGATCTGCCTGATTGCCGAAGAAGAGCAACCGAAGCTGATGGTAATTGACTCGATCCAGGTGATGCATATGGCGGATGTACAGTCATCGCCTGGTAGCGTGGCGCAGGTGCGt                                                                                                                             <  1:775989/150‑1 (MQ=255)
                         aCAATCTCAATATGTTGTCGGAAACCAGCATCGAACAGATCTGCCTGATTGCCGAAGAAGAGCAACCGAAGCTGATGGTAATTGACTCGATCCAGGTGATGCATATGGCGGATGTACAGTCATCGCCTGGTAGCGTGGCGCAGGTGCGTg                                                                                                                            <  2:2746128/150‑1 (MQ=255)
                         aCAATCTCAATATGTTGTCGGAAACCAGCATCGAACAGATCTGCCTGATTGCCGAAGAAGAGCAACCGAAGCTGATGGTAATTGACTCGATCCAGGTGATGCATATGGCGGATGTACAGTCATCGCCTGGTAGCGTGGCGCAGGTGCGTg                                                                                                                            <  2:584969/150‑1 (MQ=255)
                          cAATCTCAATATGTTGTCGGAAACCAGCATCGAACAGATCTGCCTGATTGCCGAAGAAGAGCAACCGAAGCTGATGGTAATTGACTCGATCCAGGTGATGCATATGGCGGATGTACAGTCATCGCCTGGTAGCGTGGCGCAGGTGCGTGa                                                                                                                           <  2:428631/150‑1 (MQ=255)
                          cAATCTCAATATGTTGTCGGAAACCAGCATCGAACAGATCTGCCTGATTGCCGAAGAAGAGCAACCGAAGCTGATGGTAATTGACTCGATCCAGGTGATGCATATGGCGGATGTACAGTCATCGCCTGGTAGCGTGGCGCAGGTGCGTGa                                                                                                                           >  1:3928976/1‑150 (MQ=255)
                            aTCTCAATATGTTGTCGGAAACCAGCATCGAACAGATCTGCCTGATTGCCGAAGAAGAGCAACCGAAGCTGATGGTAATTGACTCGATCCAGGTGATGCATATGGCGGATGTACAGTCATCGCCTGGTAGCGTGGCGCAGGTGCGTGaaa                                                                                                                         >  2:1337690/1‑150 (MQ=255)
                             tctcAATATGTTGTCGGAAACCAGCATCGAACAGATCTGCCTGATTGCCGAAGAAGAGCAACCGAAGCTGATGGTAATTGACTCGATCCAGGTGATGCATATGGCGGATGTACAGTCATCGCCTGGTAGCGTGGCGCAGGTGCGTGAAAc                                                                                                                        <  1:3444025/150‑1 (MQ=255)
                              ctcAATATGTTGTCGGAAACCAGCATCGAACAGATCTGCCTGATTGCCGAAGAAGAGCAACCGAAGCTGATGGTAATTGACTCGATCCAGGTGATGCATATGGCGGATGTACAGTCATCGCCTGGTAGCGTGGCGCAGGTGCGTGAAAcg                                                                                                                       <  1:2206557/150‑1 (MQ=255)
                              ctcAATATGTTGTCGGAAACCAGCATCGAACAGATCTGCCTGATTGCCGAAGAAGAGCAACCGAAGCTGATGGTAATTGACTCGATCCAGGTGATGCATATGGCGGATGTACAGTCATCGCCTGGTAGCGTGGCGCAGGTGCGTGAAAcg                                                                                                                       >  2:2526787/1‑150 (MQ=255)
                               tcAATATGTTGTCGGAAACCAGCATCGAACAGATCTGCCTGATTGCCGAAGAAGAGCAACCGAAGCTGATGGTAATTGACTCGATCCAGGTGATGCATATGGCGGATGTACAGTCATCGCCTGGTAGCGTGGCGCAGGTGCGTGAAAcgg                                                                                                                      >  1:663696/1‑150 (MQ=255)
                                cattatTTTGTCGGAAACCAGCATCGAACCGTTCTGCCTGATTGCCGAAGAAGAGCATCCTAAGCTGATTGTAATTTACTCGATCCAGGTGATGCATATGGCGGATGTTCAGTGATCGCCTGGTAGCGTGGCGCAGGTGGGTGTAAcgtg                                                                                                                     <  2:4287396/147‑3 (MQ=255)
                                 aaTATGTTGTCGGAAACCAGCATCGAACAGATCTGCCTGATTGCCGAAGAAGAGCAACCGAAGCTGATGGTAATTGACTCGATCCAGGTGATGCATATGGCGGATGTACAGTCATCGCCTGGTAGCGTGGCGCAGGTGCGTGAAAcggcg                                                                                                                    >  1:1134708/1‑150 (MQ=255)
                                  atatGTTGTCGGAAACCAGCATCGAACAGATCTGCCTGATTGCCGAAGAAGAGCAACCGAAGCTGATGGTAATTGACTCGATCCAGGTGATGCATATGGCGGATGTACAGTCATCGCCTGGTAGCGTGGCGCAGGTGCGTGAAAcggcgg                                                                                                                   >  2:95758/1‑150 (MQ=255)
                                  atatGTTGTCGGAAACCAGCATCGAACAGATCTGCCTGATTGCCGAAGAAGAGCAACCGAAGCTGATGGTAATTGACTCGATCCAGGTGATGCATATGGCGGATGTACAGTCATCGCCTGGTAGCGTGGCGCAGGTGCGTGAAAcggcgg                                                                                                                   <  2:3430927/150‑1 (MQ=255)
                                      gttgtCGGAAACCAGCATCGAACAGATCTGCCTGATTGCCGAAGAAGAGCAACCGAAGCTGATGGTAATTGACTCGATCCAGGTGATGCATATGGCGGATGTACAGTCATCGCCTGGTAGCGTGGCGCAGGTGCGTGAAACGGCGGCTTa                                                                                                               <  1:2160919/150‑1 (MQ=255)
                                       ttgtCGGAAACCAGCATCGAACAGATCTGCCTGATTGCCGAAGAAGAGCAACCGAAGCTGATGGTAATTGACTCGATCCAGGTGATGCATATGGCGGATGTACAGTCATCGCCTGGTAGCGTGGCGCAGGTGCGTGAAACGGCGGCTTAt                                                                                                              <  2:445099/150‑1 (MQ=255)
                                       ttgtCGGAAACCAGCATCGAACAGATCTGCCTGATTGCCGAAGAAGAGCAACCGAAGCTGATGGTAATTGACTCGATCCAGGTGATGCATATGGCGGATGTACAGTCATCGCCTGGTAGCGTGGCGCAGGTGCGTGAAACGGCGGCTTAt                                                                                                              <  1:48741/150‑1 (MQ=255)
                                        tgtCGGAAACCAGCATCGAACAGATCTGCCTGATTGCCGAAGAAGAGCAACCGAAGCTGATGGTAATTGACTCGATCCAGGTGATGCATATGGCGGATGTACAGTCATCGCCTGGTAGCGTGGCGCAGGTGCGTGAAACGGCGGCTTAtt                                                                                                             <  1:3343303/150‑1 (MQ=255)
                                                aCCAGCATCGAACAGATCTGCCTGATTGCCGAAGAAGAGCAACCGAAGCTGATGGTAATTGACTCGATCCAGGTGATGCATATGGCGGATGTACAGTCATCGCCTGGTAGCGTGGCGCAGGTGCGTGAAACGGCGGCTTATTTGACACGc                                                                                                     >  2:1923109/1‑150 (MQ=255)
                                                aCCAGCATCGAACAGATCTGCCTGATTGCCGAAGAAGAGCAACCGAAGCTGATGGTAATTGACTCGATCCAGGTGATGCATATGGCGGATGTACAGTCATCGCCTGGTAGCGTGGCGCAGGTGCGTGAAACGGCGGCTTATTTGACACGc                                                                                                     >  1:3381373/1‑150 (MQ=255)
                                                  cAGCATCGAACAGATCTGCCTGATTGCCGAAGAAGAGCAACCGAAGCTGATGGTAATTGACTCGATCCAGGTGATGCATATGGCGGATGTACAGTCATCGCCTGGTAGCGTGGCGCAGGTGCGTGAAACGGCGGCTTATTTGACACGCtt                                                                                                   <  1:2556754/150‑1 (MQ=255)
                                                   aGCATCGAACAGATCTGCCTGATTGCCGAAGAAGAGCAACCGAAGCTGATGGTAATTGACTCGATCCAGGTGATGCATATGGCGGATGTACAGTCATCGCCTGGTAGCGTGGCGCAGGTGCGTGAAACGGCGGCTTATTTGACACGCTTc                                                                                                  >  1:2268333/1‑150 (MQ=255)
                                                      aTCGAACAGATCTGCCTGATTGCCGAAGAAGAGCAACCGAAGCTGATGGTAATTGACTCGATCCAGGTGATGCATATGGCGGATGTACAGTCATCGCCTGGTAGCGTGGCGCAGGTGCGTGAAACGGCGGCTTATTTGACACGCTTCGcc                                                                                               >  1:246210/1‑150 (MQ=255)
                                                         gAACAGATCTGCCTGATTGCCGAAGAAGAGCAACCGAAGCTGATGGTAATTGACTCGATCCAGGTGATGCATATGGCGGATGTACAGTCATCGCCTGGTAGCGTGGCGCAGGTGCGTGAAACGGCGGCTTATTTGACACGCTTCGCCaaa                                                                                            >  1:2861047/1‑150 (MQ=255)
                                                         gAACAGATCTGCCTGATTGCCGAAGAAGAGCAACCGAAGCTGATGGTAATTGACTCGATCCAGGTGATGCATATGGCGGATGTACAGTCATCGCCTGGTAGCGTGGCGCAGGTGCGTGAAACGGCGGCTTATTTGACACGCTTCGCCaaa                                                                                            >  2:349891/1‑150 (MQ=255)
                                                         gAACAGATCTGCCTGATTGCCGAAGAAGAGCAACCGAAGCTGATGGTAATTGACTCGATCCAGGTGATGCATATGGCGGATGTACAGTCATCGCCTGGTAGCGTGGCGCAGGTGCGTGAAACGGCGGCTTATTTGACACGCTTCGCCaaa                                                                                            >  2:348208/1‑150 (MQ=255)
                                                           aCAGATCTGCCTGATTGCCGAAGAAGAGCAACCGAAGCTGATGGTAATTGACTCGATCCAGGTGATGCATATGGCGGATGTACAGTCATCGCCTGGTAGCGTGGCGCAGGTGCGTGAAACGGCGGCTTATTTGACACGCTTCGCCAAAAc                                                                                          <  2:326547/150‑1 (MQ=255)
                                                               aTCTGCCTGATTGCCGAAGAAGAGCAACCGAAGCTGATGGTAATTGACTCGATCCAGGTGATGCATATGGCGGATGTACAGTCATCGCCTGGTAGCGTGGCGCAGGTGCGTGAAACGGCGGCTTATTTGACACGCTTCGCCAAAAcgcgc                                                                                      >  2:1491668/1‑150 (MQ=255)
                                                                 cTGCCTGATTGCCGAAGAAGAGCAACCGAAGCTGATGGTAATTGACTCGATCCAGGTGATGCATATGGCGGATGTACAGTCATAGCCTGGTAGCGTGGCGCAGGTGCGTGAAACGGCGGCTTATTTGACACGCTTCGCCAAAACGCGCgg                                                                                    >  1:707993/1‑150 (MQ=255)
                                                                   gCCTGATTGCCGAAGAAGAGCAACCGAAGCTGATGGTAATTGACTCGATCCAGGTGATGCATATGGCGGATGTACAGTCATCGCCTGGTAGCGTGGCGCAGGTGCGTGAAACGGCGGCTTATTTGACACGCTTCGCCAAAACGCGCGgtg                                                                                  <  1:2610240/150‑1 (MQ=255)
                                                                    ccTGATTGCCGAAGAAGAGCAACCGAAGCTGATGGTAATTGACTCGATCCAGGTGATGCATATGGCGGATGTACAGTCATCGCCTGGTAGCGTGGCGCAGGTGCGTGAAACGGCGGCTTATTTGACACGCTTCGCCAAAACGCGCGgtgt                                                                                 >  2:282343/1‑150 (MQ=255)
                                                                     cTGATTGCCGAAGAAGAGCAACCGAAGCTGATGGTAATTGACTCGATCCAGGTGATGCATATGGCGGATGTACAGTCATCGCCTGGTAGCGTGGCGCAGGTGCGTGAAACGGCGGCTTATTTGACACGCTTCGCCAAAACGCGCGGtgtg                                                                                >  1:1874959/1‑150 (MQ=255)
                                                                     cTGATTGCCGAAGAAGAGCAACCGAAGCTGATGGTAATTGACTCGATCCAGGTGATGCATATGGCGGATGTACAGTCATCGCCTGGTAGCGTGGCGCAGGTGCGTGAAACGGCGGCTTATTTGACACGCTTCGCCAAAACGCGCGGtgtg                                                                                >  1:4003670/1‑150 (MQ=255)
                                                                      tGATTGCCGAAGAAGAGCAACCGAAGCTGATGGTAATTGACTCGATCCAGGTGATGCATATGGCGGATGTACAGTCATCGCCTGGTAGCGTGGCGCAGGTGCGTGAAACGGCGGCTTATTTGACACGCTTCGCCAAAACGCGCGGTGTgg                                                                               >  1:1966030/1‑150 (MQ=255)
                                                                      tGATTGCCGAAGAAGAGCAACCGAAGCTGATGGTAATTGACTCGATCCAGGTGATGCATATGGCGGATGTACAGTCATCGCCTGGTAGCGTGGCGCAGGTGCGTGAAACGGCGGCTTATTTGACACGCTTCGCCAAAACGCGCGGTGTgg                                                                               >  1:974491/1‑150 (MQ=255)
                                                                        aTTGCCGAAGAAGAGCAACCGAAGCTGATGGTAATTGACTCGATCCAGGTGATGCATATGGCGGATGTACAGTCATCGCCTGGTAGCGTGGCGCAGGTGCGTGAAACGGCGGCTTATTTGACACGCTTCGCCAAAACGCGCGGTGTGGCg                                                                             <  2:314400/150‑1 (MQ=255)
                                                                        aTTGCCGAAGAAGAGCAACCGAAGCTGATGGTAATTGACTCGATCCAGGTGATGCATATGGCGGATGTACAGTCATCGCCTGGTAGCGTGGCGCAGGTGCGTGAAACGGCGGCTTATTTGACACGCTTCGCCAAAACGCGCGGTGTGGCg                                                                             >  2:1763177/1‑150 (MQ=255)
                                                                         ttGCCGAAGAAGAGCAACCGAAGCTGATGGTAATTGACTCGATCCAGGTGATGCATATGGCGGATGTACAGTCATCGCCTGGTAGCGTGGCGCAGGTGCGTGAAACGGCGGCTTATTTGACACGCTTCGCCAAAACGCGCGGTGTGGCGa                                                                            <  1:3726100/150‑1 (MQ=255)
                                                                          tGCCGAAGAAGAGCAACCGAAGCTGATGGTAATTGACTCGATCCAGGTGATGCATATGGCGGATGTACAGTCATCGCCTGGTAGCGTGGCGCAGGTGCGTGAAACGGCGGCTTATTTGACACGCTTCGCCAAAACGCGCGGTGTGGCGAt                                                                           >  1:697407/1‑150 (MQ=255)
                                                                            ccGAAGATGAGCAACCGAAGCTGATGGTTATTGACTCGATCCAGGTGATGCATATGGCGGATGTACAGTCATCGCCTGGTAGCGTGGCGCAGGTGCGTGATACGGCGGCTTATTTGACACGCTTCGCCAAAACGCGCGGTGTGGCGATTg                                                                         <  1:4349101/150‑1 (MQ=255)
                                                                            ccGAAGAAGAGCAACCGAAGCTGATGGTAATTGACTCGATCCAGGTGATGCATATGGCGGATGTACAGTCATCGCCTGGTAGCGTGGCGCAGGTGCGTGAAACGGCGGCTTATTTGACACGCTTCGCCAAAACGCGCGGTGTGGCGATTg                                                                         <  2:2195953/150‑1 (MQ=255)
                                                                            ccGAAGAAGAGCAACCGAAGCTGATGGTAATTGACTCGATCCAGGTGATGCATATGGCGGATGTACAGTCATCGCCTGGTAGCGTGGCGCAGGTGCGTGAAACGGCGGCTTATTTGACACGCTTCGCCAAAACGCGCGGTGTGGCGATTg                                                                         <  1:2367588/150‑1 (MQ=255)
                                                                             cGAAGAAGAGCAACCGAAGCTGATGGTAATTGACTCGATCCAGGTGATGCATATGGCGGATGTACAGTCATCGCCTGGTAGCGTGGCGCAGGTGCGTGAAACGGCGGCTTATTTGACACGCTTCGCCAAAACGCGCGGTGTGGCGATTGt                                                                        >  1:1497755/1‑150 (MQ=255)
                                                                               aagaagAGCAACCGAAGCTGATGGTAATTGACTCGATCCAGGTGATGCATATGGCGGATGTACAGTCATCGCCTGGTAGCGTGGCGCAGGTGCGTGAAACGGCGGCTTATTTGACACGCTTCGCCAAAACGCGCGGTGTGGCGATTGTCa                                                                      >  1:1493201/1‑150 (MQ=255)
                                                                               aagaagAGCAACCGAAGCTGATGGTAATTGAATCGATCCAGTTGAAGCATATGGCGGATGTACAGTCATCGCCTGGTAGCGTGGCGAAGGTGCGTGACACGGCTGCTTATTTGACACGCTTCGCCAAAACGCGCGCTGTGGCGATTGTCa                                                                      >  1:4423460/1‑150 (MQ=255)
                                                                                 gaagaGCAACCGAAGCTGATGGTAATTGACTCGATCCAGGTGATGCATATGGCGGATGTACAGTCATCGCCTGGTAGCGTGGCGCAGGTGCGTGAAACGGCGGCTTATTTGACACGCTTCGCCAAAACGCGCGGTGTGGCGATTGTCAtg                                                                    >  1:1374746/1‑150 (MQ=255)
                                                                                  aagaGCAACCGAAGCTGATGGTAATTGACTCGATCCAGGTGATGCATATGGCGGATGTACAGTCATCGCCTGGTAGCGTGGCGCAGGTGCGTGAAACGGCGGCTTATTTGACACGCTTCGCCAAAACGCGCGGTGTGGCGATTGTCAtgg                                                                   <  1:909798/150‑1 (MQ=255)
                                                                                   agagCAACCGAAGCTGATGGTAATTGACTCGATCCAGGTGATGCATATGGCGGATGTACAGTCATCGCCTGGTAGCGTGGCGCAGGTGCGTGAAACGGCGGCTTATTTGACACGCTTCGCCAAAACGCGCGGTGTGGCGATTGTCAtggt                                                                  >  1:3464119/1‑150 (MQ=255)
                                                                                   agagCAACCGAAGCTGATGGTAATTGACTCGATCCAGGTGATGCATATGGCGGATGTACAGTCATCGCCTGGTAGCGTGGCGCAGGTGCGTGAAACGGCGGCTTATTTGACACGCTTCGCCAAAACGCGCGGTGGGGCGATTGTCAtggt                                                                  >  1:1178142/1‑150 (MQ=255)
                                                                                       cAACCGAAGCTGATGGTAATTGACTCGATCCAGGTGATGCATATGGCGGATGTACAGTCATCGCCTGGTAGCGTGGCGCAGGTGCGTGAAACGGCGGCTTATTTGACACGCTTCGCCAAAACGCGCGGTGTGGCGATTGTCATGGTgggg                                                              <  1:244655/150‑1 (MQ=255)
                                                                                        aaCCGAAGCTGATGGTAATTGACTCGATCCAGGTGATGCATATGGCGGATGTACAGTCATCGCCTGGTAGCGTGGCGCAGGTGCGTGAAACGGCGGCTTATTTGACACGCTTCGCCAAAACGCGCGGTGTGGCGATTGTCATGGTGGGGc                                                             >  2:1618999/1‑150 (MQ=255)
                                                                                        aaCCGAAGCTGATGGTAATTGACTCGATCCAGGTGATGCATATGGCGGATGTACAGTCATCGCCTGGTAGCGTGGCGCAGGTGCGTGAAACGGCGGCTTATTTGACACGCTTCGCCAAAACGCGCGGTGTGGCGATTGTCATGGTGGGGc                                                             >  2:1294978/1‑150 (MQ=255)
                                                                                        aaCCGAAGCTGATGGTAATTGACTCGATCCAGGTGATGCATATGGCGGATGTACAGTCATCGCCTGGTAGCGTGGCGCAGGTGCGTGAAACGGCGGCTTATTTGACACGCTTCGCCAAAACGCGCGGTGTGGCGATTGTCATGGTGGGGc                                                             >  2:1459745/1‑150 (MQ=255)
                                                                                          ccGAAGCTGATGGTAATTGACTCGATCCAGGTGATGCATATGGCGGATGTACAGTCATCGCCTGGTAGCGTGGCGCAGGTGCGTGAAACGGCGGCTTATTTGACACGCTTCGCCAAAACGCGCGGTGTGGCGATTGTCATGGTGGGGCAc                                                           >  1:4081558/1‑150 (MQ=255)
                                                                                                cTGATGGTAATTGACTCTATCCAGGTGATGCATATGGCGGATGTACAGTCATCGCCTGGTAGCGTGGCGCAGGTGCGTGAAACGGCGGCTTATTTGACACGCTTCGCCAAAACGCGCGGTGTGGCGATTGTCATGGTGGGGCACGTAAcc                                                     <  1:3604972/150‑1 (MQ=255)
                                                                                                cTGATGGTAATTGACTCGATCCAGGTGATGCATATGGCGGATGTACAGTCATCGCCTGGTAGCGTGGCGCAGGTGCGTGAAACGGCGGCTTATTTGACACGCTTCGCCAAAACGCGCGGTGTGGCGATTGTCATGGTGGGGCACGTAAcc                                                     >  1:799183/1‑150 (MQ=255)
                                                                                                cTGATGGTAATTGACTCGATCCAGGTGATGCATATGGCGGATGTACAGTCATCGCCTGGTAGCGTGGCGCAGGTGCGTGAAACGGCGGCTTATTTGACACGCTTCGCCAAAACGCGCGGTGTGGCGATTGTCATGGTGGGGCACGTAAcc                                                     <  2:3466211/150‑1 (MQ=255)
                                                                                                 tGATGGTAATTGACTCGATCCAGGTGATGCATATGGCGGATGTACAGTCATCGCCTGGTAGCGTGGCGCAGGTGCGTGAAACGGCGGCTTATTTGACACGCTTCGCCAAAACGCGCGGTGTGGCGATTGTCATGGTGGGGCACGTAACCa                                                    >  2:2891400/1‑150 (MQ=255)
                                                                                                  gATGGTAATTGACTCGATCCAGGTGATGCATATGGCGGATGTACAGTCATCGCCTGGTAGCGTGGCGCAGGTGCGTGAAACGGCGGCTTATTTGACACGCTTCGCCAAAACGCGCGGTGTGGCGATTGTCATGGTGGGGCACGTAACCaa                                                   >  2:35670/1‑150 (MQ=255)
                                                                                                        aaTTGACTCGATCCAGGTGATGCATATGGCGGATGTACAGTCATCGCCTGGTAGCGTGGCGCAGGTGCGTGAAACGGCGGCTTATTTGACACGCTTCGCCAAAACGCGCGGTGTGGCGATTGTCATGGTGGGGCACGTAACCAAAGATgg                                             <  2:2872081/150‑1 (MQ=255)
                                                                                                        aaTTGACTCGATCCAGGTGATGCATATGGCGGATGTACAGTCATCGCCTGGTAGCGTGGCGCAGGTGCGTGAAACGGCGGCTTATTTGACACGCTTCGCCAAAACGCGCGGTGTGGCGATTGTCATGGTGGGGCACGTAACCAAAGATgg                                             >  2:3065995/1‑150 (MQ=255)
                                                                                                        aaTTGACTCGATCCAGGTGATGCATATGGCGGATGTACAGTCATCGCCTGGTAGCGTGGCGCAGGTGCGTGAAACGGCGGCTTATTTGACACGCTTCGCCAAAACGCGCGGTGTGGCGATTGTCATGGTGGGGCACGTAACCAAAGATgg                                             >  1:3034484/1‑150 (MQ=255)
                                                                                                           tGACTCGATCCAGGTGATGCATATGGCGGATGTACAGTCATCGCCTGGTAGCGTGGCGCAGGTGCGTGAAACGGCGGCTTATTTGACACGCTTCGCCAAAACGCGCGGTGTGGCGATTGTCATGGTGGGGCACGTAACCAAAGATGGTTc                                          <  2:2423744/150‑1 (MQ=255)
                                                                                                           tGACTCGATCCAGGTGATGCATATGGCGGATGTACAGTCATCGCCTGGTAGCGTGGCGCAGGTGCGTGAAACGGCGGCTTATTTGACACGCTTCGCCAAAACGCGCGGTGTGGCGATTGTCATGGTGGGGCACGTAACCAAAGATGGTTc                                          <  2:2170512/150‑1 (MQ=255)
                                                                                                           tGACTCGATCCAGGTGATGCATATGGCGGATGTACAGTCATCGCCTGGTAGCGTGGCGCAGGTGCGTGAAACGGCGGCTTATTTGACACGCTTCGCCAAAACGCGCGGTGTGGCGATTGTCATGGTGGGGCACGTAACCAAAGATGGTTc                                          <  2:681554/150‑1 (MQ=255)
                                                                                                             aCTCGATCCAGGTGATGCATATGGCGGATGTACAGTCATCGCCTGGTAGCGTGGCGCAGGTGCGTGAAACGGCGGCTTATTTGACACGCTTCGCCAAAACGCGCGGTGTGGCGATTGTCATGGTGGGGCACGTAACCAAAGATGGTTCgc                                        >  2:4117027/1‑150 (MQ=255)
                                                                                                               tCGATCCAGGTGATGCATATGGCGGATGTACAGTCATCGCCTGGTAGCGTGGCGCAGGTGCGTGAAACGGCGGCTTATTTGACACGCTTCGCCAAAACGCGCGGTGTGGCGATTGTCATGGTGGGGCACGTAACCAAAGATGGTTCgctg                                      <  2:2460847/150‑1 (MQ=255)
                                                                                                                 gATCCAGGTGATGCATATGGCGGATGTACAGTCATAGCCTGGTAGCGTGGCGCAGGTGCGTGAAACGGCGGCTTATTTGACACGCTTCGCCAAAACGCGCGGTGTGGCGATTGTCATGGTGGGGCACGTAACCAAAGATGGTTCgctggc                                    >  1:1156690/1‑150 (MQ=255)
                                                                                                                  tgccAGGTGATGCATATGGCGGATGTACTGTCTTCGCCTTGGAGCGTGGCGCAGGTGCGTGTTACGGCGTCTTATTTGACACGATTCGCCAAATCGCGCGGTGTGGCGTTTGTCATGGTGGGTCACGTAACCAAAGATGGTTCgatgtct                                   <  2:2913582/148‑1 (MQ=255)
                                                                                                                    ccAGGTGATGCATATGGCGGATGTACAGTCATCGCCTGGTAGCGTGGCGCAGGTGCGTGAAACGGCGGCTTATTTGACACGCTTCGCCAAAACGCGCGGTGTGGCGATTGTCATGGTGGGGCACGTAACCAAAGATGGTTCGctggctgg                                 >  1:566222/1‑150 (MQ=255)
                                                                                                                      aGGTGATGCATATGGCGGATGTACAGTCATAGCCTGGTAGCGTGGCGCAGGTGCGTGAAACGGCGGCTTATTTGACACGCTTCGCCAAAACGCGCGGTGTGGCGATTGTCATGGTGGGGCACGTAACCAAAGATGGTTCGCTGGCTGGcc                               >  2:1136759/1‑150 (MQ=255)
                                                                                                                        gTGATGCATATGGCGGATGTACAGTCATCGCCTGGTAGCGTGGCGCAGGTGCGTGAAACGGCGGCTTATTTGACACGCTTCGCCAAAACGCGCGGTGTGGCGATTGTCATGGTGGGGCACGTAACCAAAGATGGTTCGCTGGCTGGCCCg                             >  1:1795671/1‑150 (MQ=255)
                                                                                                                         tGATGCATATGGCGGATGTACAGTCATCGCCTGGTAGCGTGGCGCAGGTGCGTGAAACGGCGGCTTATTTGACACGCTTCGCCAAAACGCGCGGTGTGGCGATTGTCATGGTGGGGCACGTAACCAAAGATGGTTCGCTGGCTGGCCCGa                            >  1:2759664/1‑150 (MQ=255)
                                                                                                                                     cGGATGTACAGTCATCGCCTGGTAGCGTGGCGCAGGTGCGTGAAACGGCGGCTTATTTGACACGCTTCGCCAAAACGCGCGGTGTGGCGATTGTCATGGTGGGGCACGTAACCAAAGATGGTTCGCTGGCTGGCCCGAAAGTGCTGGAac                <  1:813674/150‑1 (MQ=255)
                                                                                                                                        aTGTACAGTCATCGCCTGGTAGCGTGGCGCAGGTGCGTGAAACGGCGGCTTATTTGACACGCTTCGCCAAAACGCGCGGTGTGGCGATTGTCATGGTGGGGCACGTAACCAAAGATGGTTCGCTGGCTGGCCCGAAAGTGCTGGAACACt             <  2:2291725/150‑1 (MQ=255)
                                                                                                                                           tACAGTCATCGCCTGGTAGCGTGGCGCAGGTGCGTGAAACGGCGGCTTATTTGACACGCTTCGCCAAAACGCGCGGTGTGGCGATTGTCATGGTGGGGCACGTAACCAAAGATGGTTCGCTGGCTGGCCCGAAAGTGCTGGAACACTGTa          >  2:604781/1‑150 (MQ=255)
                                                                                                                                             cAGTCATCGCCTGGTAGCGTGGCGCAGGTGCGTGAAACGGCGGCTTATTTGACACGCTTCGCCAAAACGCGCGGTGTGGCGATTGTCATGGTGGGGCACGTAACCAAAGATGGTTCGCTGGCTGGCCCGAAAGTGCTGGAACACTGTATc        <  2:4517187/150‑1 (MQ=255)
                                                                                                                                             cAGTCATCGCCTGGTAGCGTGGCGCAGGTGCGTGAAACGGCGGCTTATTTGACACGCTTCGCCAAAACGCGCGGTGTGGCGATTGTCATGGTGGGGCACGTAACCAAAGATGGTTCGCTGGCTGGCCCGAAAGTGCTGGAACACTGTATc        <  1:2029453/150‑1 (MQ=255)
                                                                                                                                             cAGTCATCGCCTGGTAGCGTGGCGCAGGTGCGTGAAACGGCGGCTTATTTGACACGCTTCGCCAAAACGCGCGGTGTGGCGATTGTCATGGTGGGGCACGTAACCAAAGATGGTTCGCTGGCTGGCCCGAAAGTGCTGGAACACTGTATc        <  1:4303436/150‑1 (MQ=255)
                                                                                                                                              aGTCATCGCCTGGTAGCGTGGCGCAGGTGCGTGAAACGGCGGCTTATTTGACACGCTTCGCCAAAACGCGCGGTGTGGCGATTGTCATGGTGGGGCACGTAACCAAAGATGGTTCGCTGGCTGGCCCGAAAGTGCTGGAACACTGTATCg       >  2:3769761/1‑150 (MQ=255)
                                                                                                                                                tCATCGCCTGGTAGCGTGGCGCAGGTGCGTGAAACGGCGGCTTATTTGACACGCTTCGCCAAAACGCGCGGTGTGGCGATTGTCATGGTGGGGCACGTAACCAAAGATGGTTCGCTGGCTGGCCCGAAAGTGCTGGAACACTGTATCGAc     >  1:1826630/1‑150 (MQ=255)
                                                                                                                                                tCATCGCCTGGTAGCGTGGCGCAGGTGCGTGAAACGGCGGCTTATTTGACACGCTTCGCCAAAACGCGCGGTGTGGCGATTGTCATGGTGGGGCACGTAACCAAAGATGGTTCGCTGGCTGGCCCGAAAGTGCTGGAACACTGTATCGAc     >  2:729732/1‑150 (MQ=255)
                                                                                                                                                 cATCGCCTGGTAGCGTGGCGCAGGTGCGTGAAACGGCGGCTTATTTGACACGCTTCGCCAAAACGCGCGGTGTGGCGATTGTCATGGTGGGGCACGTAACCAAAGATGGTTCGCTGGCTGGCCCGAAAGTGCTGGAACACTGTATCGACt    <  2:2621181/150‑1 (MQ=255)
                                                                                                                                                 cATCGCCTGGTAGCGTGGCGCAGGTGCGTGAAACGGCGGCTTATTTGACACGCTTCGCCAAAACGCGCGGTGTGGCGATTGTCATGGTGGGGCACGTAACCAAAGATGGTTCGCTGGCTGGCCCGAAAGTGCTGGAACACTGTATCGACt    >  1:3129606/1‑150 (MQ=255)
                                                                                                                                                  aTCGCCTGGTAGCGTGGCGCAGGTGCGTGAAACGGCGGCTTATTTGACACGCTTCGCCAAAACGCGCGGTGTGGCGATTGTCATGGTGGGGCACGTAACCAAAGATGGTTCGCTGGCTGGCCCGAAAGTGCTGGAACACTGTATCGACTg   >  2:3853607/1‑150 (MQ=255)
                                                                                                                                                   tCGCCTGGTAGCGTGGCGCAGGTGCGTGAAACGGCGGCTTATTTGACACGCTTCGCCAAAACGCGCGGTGTGGCGATTGTCATGGTGGGGCACGTAACCAAAGATGGTTCGCTGGCTGGCCCGAAAGTGCTGGAACACTGTATCGACTGt  <  2:2313753/150‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
GCTCATCGCCTTGGCCTGCCGACTGACAATCTCAATATGTTGTCGGAAACCAGCATCGAACAGATCTGCCTGATTGCCGAAGAAGAGCAACCGAAGCTGATGGTAATTGACTCGATCCAGGTGATGCATATGGCGGATGTACAGTCATCGCCTGGTAGCGTGGCGCAGGTGCGTGAAACGGCGGCTTATTTGACACGCTTCGCCAAAACGCGCGGTGTGGCGATTGTCATGGTGGGGCACGTAACCAAAGATGGTTCGCTGGCTGGCCCGAAAGTGCTGGAACACTGTATCGACTGT  >  CP009273/4616149‑4616445

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑