BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	418,995	T→G	8.6%	L341R (CTG→CGG)	malZ →	maltodextrin glucosidase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	418,995	0	T	G	8.6%	644.4 / 7.6	265	L341R (CTG→CGG)	malZ	maltodextrin glucosidase
Reads supporting (aligned to +/- strand): ref base T (135/98); new base G (2/30); total (137/128)
Fisher's exact test for biased strand distribution p-value = 1.17e-08
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 270 total aligned reads displayed.

CTGGCTTGGCTATGCCAGCTTGCCGAAGCTGGATTATCAGTCGGAAAGTCTGGTGAATGAAATTTATCGCGGGGAAGACAGTATTGTCCGCCACTGGCTGAAAGCGCCGTGGAATATGGACGGCTGGCGGCTGGATGTGGTGCATATGCTGGGGGAGGCGGGTGGGGCGCGCAATAATATGCAGCACGTTGCCGGGATCACCGAAGCGGCGAAAGAAACCCAGCCGGAAGCGTATATTGTCGGCGAACATTTTGGCGATGCACGGCAATGGTTACAGGCCGATGTGGAAGATGCCGCCA  >  CP009273/418846‑419144

cTGGCTTGGCTATGCCAGCTTGCCGAAGCTGGATTATCAGTCGGAAAGTCTGGTGAATGAAATTTATCGCGGGGAAGACAGTATTGTCCGCCACTGGCTGAAAGCGCCGTGGAATATGGACGGCTGGCGGCTGGATGTGGTGCATATGCt                                                                                                                                                       <  2:4328159/150‑1 (MQ=255)

  ggcttggctATGCCAGCTTGCCGAAGCTGGATTATCAGTCGGAAAGTCTGGTGAATGAAATTTATCGCGGGGAAGACAGTATTGTCCGCCACTGGCTGAAAGCGCCGTGGAATATGGACGGCTGGCGGCTGGATGTGGTGCATATGCTgg                                                                                                                                                     <  1:722121/150‑1 (MQ=255)

   gcttggctATGCCAGCTTGCCGAAGCTGGATTATCAGTCGGAAAGTCTGGTGAATGAAATTTATCGCGGGGAAGACAGTATTGTCCGCCACTGGCTGAAAGCGCCGTGGAATATGGACGGCTGGCGGCTGGATGTGGTGCATATGCTggg                                                                                                                                                    <  2:875767/150‑1 (MQ=255)

   gcttggctATGCCAGCTTGCCGAAGCTGGATTATCAGTCGGAAAGTCTGGTGAATGAAATTTATCGCGGGGAAGACAGTATTGTCCGCCACTGGCTGAAAGCGCCGTGGAATATGGACGGCTGGCGGCTGGATGTGGTGCATATGCTggg                                                                                                                                                    >  1:3085045/1‑150 (MQ=255)

   gcttggctATGCCAGCTTGCCGAAGCTGGATTATCAGTCGGAAAGTCTGGTGAATGAAATTTATCGCGGGGAAGACAGTATTGTCCGCCACTGGCTGAAAGCGCCGTGGAATATGGACGGCTGGCGGCTGGATGTGGTGCATATGCTggg                                                                                                                                                    <  2:804639/150‑1 (MQ=255)

    cttggctATGCCAGCTTGCCGAAGCTGGATTATCAGTCGGAAAGTCTGGTGAATGAAATTTATCGCGGGGAAGACAGTATTGTCCGCCACTGGCTGAAAGCGCCGTGGAATATGGACGGCTGGCGGCTGGATGTGGTGCATATGCTgggg                                                                                                                                                   <  2:2418642/150‑1 (MQ=255)

     ttggctATGCCAGCTTGCCGAAGCTGGATTATCAGTCGGAAAGTCTGGTGAATGAAATTTATCGCGGGGAAGACAGTATTGTCCGCCACTGGCTGAAAGCGCCGTGGAATATGGACGGCTGGCGGCTGGATGTGGTGCATATGCTggggg                                                                                                                                                  >  2:4408395/1‑150 (MQ=255)

      tggctATGCCAGCTTGCCGAAGCTGGATTATCAGTCGGAAAGTCTGGTGAATGAAATTTATCGCGGGGAAGACAGTATTGTCCGCCACTGGCTGAAAGCGCCGTGGAATATGGACGGCTGGCGGCTGGATGTGGTGCATATGCTGGGGGa                                                                                                                                                 >  2:1382159/1‑150 (MQ=255)

        gctATGCCAGCTTGCCGAAGCTGGATTATCAGTCGGAAAGTCTGGTGAATGAAATTTATCGCGGGGAAGACAGTATTGTCCGCCACTGGCTGAAAGCGCCGTGGAATATGGACGGCTGGCGGCTGGATGTGGTGCATATGCTGGGGGAgg                                                                                                                                               <  1:2814810/150‑1 (MQ=255)

           aTGCCAGCTTGCCGAAGCTGGATTATCAGTCGGAAAGTCTGGTGAATGAAATTTATCGCGGGGAAGACAGTATTGTCCGCCACTGGCTGAAAGCGCCGTGGAATATGGACGGCTGGCGGCTGGATGTGGTGCATATGCTGGGGGAGGCgg                                                                                                                                            <  1:1735653/150‑1 (MQ=255)

           aTGCAAGCTTGCCGAAGCAGGATTATCAGTCGGAAAGTCTGGTGAATGAAATTTATCGCGGGGAAGACCGTATTGTCCGCCACAGGCTGAAATCGCCGTGGAATATGGACGTCTGGCGGCTGGATGTGGAGCATATTCTGGGGGAGGCgg                                                                                                                                            >  2:654848/1‑150 (MQ=255)

             gCCAGCTTGCCGAAGCTGGATTATCAGTCGGAAAGTCTGGTGAATGAAATTTATCGCGGGGAAGACAGTATTGTCCGCCACTGGCTGAAAGCGCCGTGGAATATGGACGGCTGGCGGCTGGATGTGGTGCATATGCTGGGGGAGGCGGGt                                                                                                                                          >  2:207344/1‑150 (MQ=255)

             gCCAGCTTGCCGAAGCTGGATTATCAGTCGGAAAGTCTGGTGAATGAAATTTATCGCGGGGAAGACAGTATTGTCCGCCACTGGCTGAAAGCGCCGTGGAATATGGACGGCTGGCGGCTGGATGTGGTGCATATGCTGGGGGAGGCGGGt                                                                                                                                          >  2:206238/1‑150 (MQ=255)

              ccAGCTTGCCGAAGCTGGATTATCAGTCGGAAAGTCTGGTGAATGAAATTTATCGCGGGGAAGACAGTATTGTCCGCCACTGGCTGAAAGCGCCGTGGAATATGGACGGCTGGCGGCTGGATGTGGTGCATATGCTGGGGGAGGCGGGTg                                                                                                                                         <  2:1685145/150‑1 (MQ=255)

              ccAGCTTGCCGAAGCTGGATTATCAGTCGGAAAGTCTGGTGAATGAAATTTATCGCGGGGAAGACAGTATTGTCCGCCACTGGCTGAAAGCGCCGTGGAATATGGACGGCTGGCGGCTGGATGTGGTGCATATGCTGGGGGAGGCGGGTg                                                                                                                                         <  2:2393238/150‑1 (MQ=255)

              ccAGCTTGCCGAAGCTGGATTATCAGTCGGAAAGTCTGGTGAATGAAATTTATCGCGGGGAAGACAGTATTGTCCGCCACTGGCTGAAAGCGCCGTGGAATATGGACGGCTGGCGGCTGGATGTGGTGCATATGCTGGGGGAGGCGGGTg                                                                                                                                         <  2:3793070/150‑1 (MQ=255)

                aGCTTGCCGAAGCTGGTTTATCAGTCGGAAAGTCTGGTGAATGAAATTTATCGCGGGGAAGACAGTATTGTCCGCCACTGGCTGAAAGCGCCGTGGAATATGGACGGCTGGCGGCTGGATGTGGTGCATATGCTGGGGGAGGCGGGTggg                                                                                                                                       <  2:738554/150‑1 (MQ=255)

                aGCTTGCCGAAGCTGGATTATCAGTCGGAAAGTCTGGTGAATGAAATTTATCGCGGGGAAGACAGTATTGTCCGCCACTGGCTGAAAGCGCCGTGGAATATGGACGGCTGGGGGCTGGATGTGGTGCATATGCTGGGGGAGGCGGGTggg                                                                                                                                       <  2:191953/150‑1 (MQ=255)

                  cTTGCCGAAGCTGGATTATCAGTCGGAAAGTCTGGTGAATGAATTTTATCGCGGGGAAGACAGTATTGTCCGCCACTGGCTGAAAGCGCCGTGGAATATGGACGGCTGGCGGCTGGATGTGGTGCATATGCTGGGGGAGGCGGGTGGGgc                                                                                                                                     <  2:128236/150‑1 (MQ=255)

                  cTTGCCGAAGCTGGATTATCAGTCGGAAAGTCTGGTGAATGAAATTTATCGCGGGGAAGACAGTATTGTCCGCCACTGGCTGAAAGCGCCGTGGAATATGGACGGCTGGCGGCTGGATGTGGTGCATATGCTGGGGGAGGCGGGTGGGgc                                                                                                                                     <  2:1543774/150‑1 (MQ=255)

                      ccGAAGCTGGATTATCAGTCGGAAAGTCTGGTGAATGAAATTTATCGCGGGGAAGACAGTATTGTCCGCCACTGGCTGAAAGCGCCGTGGAATATGGACGGCTGGCGGCTGGATGTGGTGCATATGCTGGGGGAGGCGGGTGGGgcgcgc                                                                                                                                 >  2:2025910/1‑150 (MQ=255)

                        gAAGCTGGATTATCAGTCGGAAAGTCTGGTGAATGAAATTTATCGCGGGGAAGACAGTATTGTCCGCCACTGGCTGAAAGCGCCGTGGAATATGGACGGCTGGCGGCTGGATGTGGTGCATATGCTGGGGGAGGCGGGTGGGGCGCGCaa                                                                                                                               >  2:3928720/1‑150 (MQ=255)

                         aaGCTGTTTTATCTGTCGTAATGTCTGGTTAATTAAATTTATCGCGGGGAAGACATTTTTGTCCGCAAGTGGCTGAAAGCGCGGTGGAATTTGGAGGGCTGGGGGGTGTTTGTGGTGATTATGCTGGGGGTGGGGGGTGGGGCGCGCaat                                                                                                                              <  1:1226802/150‑1 (MQ=255)

                         aaGCTGGTTTATCATTCGGAAAGTCTGTTTAATGAAATTTATCGCGGGGAAGAAAGTATTGTCCGCCACTGGCGGAAAGCGCCGTGGAATATGGACGGCTGGCGGCTGGATGTGGTGATTATGCTGGGGGAGGCGGGTGGGGCGCGCaat                                                                                                                              <  2:3052713/150‑1 (MQ=255)

                         aaGCTGGATTATCAGTCGGAAAGTCTGGTGAATGAAATTTATCGCGGGGAATACAGTATTGTCCGCCACTGGCTGAAAGCGCCGTGGAATATGGACGGCTGGCGGCTGGATGTGGTGCATATGATGTGTGAGGCGGGTGGGGCGCGCaat                                                                                                                              >  1:1258189/1‑150 (MQ=255)

                            cTGGATTATCAGTCGGAAAGTCTGGTGAATGAAATTTATCGCGGGGAAGACAGTATTGTCCGCCACTGGCTGAAAGCGCCGTGGAATATGGACGGCTGGCGGCTGGATGTGGTGCATATGCTGGGGGAGGCGGGTGGGGCGCGCaataat                                                                                                                           <  2:3465780/150‑1 (MQ=255)

                             tgtaTTATCAGTCGGAATGTCTGGTTATTGAATTTTATCGCGGGGAAGACTGTTTTGTCCGCCACTGGCTTAAAGCGCCGTGGAATTTGGACGGCTGGCGGCTGGATGTGTTGAATATGCTGGGGGTGGCGGGTGGGGCGCGCAataata                                                                                                                          <  1:975300/147‑1 (MQ=255)

                             tGGATTATCAGTCGGAAAGTCTGGTGAATGAAATTTATCGCGGGGAAGACAGTATTGTCCTCCACTGGCTGAAAGAGCCGTGGAATATGGACGGCTGGCGGCTGGATGTGGTGCATATGCTGGGGGAGGCGGGTGGGGCGCGCAataata                                                                                                                          >  2:3657514/1‑150 (MQ=255)

                              ggATTATCAGTCGGAAAGTCTGGTGAATGAAATTTATCGCGGGGAAGACAGTATTGTCCGCCACTGGCTGAAAGCGCCGTGGAATATGGACGGCTGGCGGCTGGATGTGGTGCATATGCTGGGGGAGGCGGGTGGGGCGCGCAATAatat                                                                                                                         >  2:3887105/1‑150 (MQ=255)

                                aTTATCAGTCGGAAAGTCTGGTGAATGAAATTTATCGCGGGGAAGACAGTATTGTCCGCCACTGGCTGAAAGCGCCGTGGAATATGGACGGCTGGCGGCTGGATGTGGTGCATATGCTGGGGGAGGCGGGTGGGGCGCGCAATAATATgc                                                                                                                       >  1:3680372/1‑150 (MQ=255)

                                      aGTCGGAATGTCTGTTGAATTAAATTTATCGCGGGGAAGACATTATTGTCCGCCACTGGCTGAAAGCGCCGTGGAATTTGGACGGCGGGCGGCTGGTTGTGGTGCATATGCTGGGGGAGGCGGGTGGGGCGCGCAATAATATGCAGCACg                                                                                                                 <  1:2496413/150‑1 (MQ=255)

                                      aGTCGGAAAGTCTGGTGAATGAAATTTATCGCGGGGAAGACAGTATTGTCCGCCACTGGCTGAAAGCGCCGTGGAATATGGACGGCTGGCGGCTGTATGTGGTGCATAGGCGGGGGGAGGCGGGTGGGGCGCGCAATAATATGCAGCACg                                                                                                                 <  1:1395407/150‑1 (MQ=255)

                                      aGTCGGAAAGTCTGGTGAATGAAATTTATCGCGGGGAAGACAGTATTGTCCGCCACTGGCTGAAAGCGCCGTGGAATATGGACGGCTGGCGGCTGGATGTGGTGCATATGCTGGGGGAGGCGGGTGGGGCGCGCAATAATATGCAGCACg                                                                                                                 >  1:1341398/1‑150 (MQ=255)

                                      aGTCGGAAAGTCTGGTGAATGAAATTTATCGCGGGGAAGACAGTATTGTCCGCCACTGGCTGAAAGCGCCGTGGAATATGGACGGCTGGCGGCTGGATGTGGTGCATATGCTGGGGGAGGCGGGTGGGGCGCGCAATAATATGCAGCACg                                                                                                                 >  1:2524797/1‑150 (MQ=255)

                                       gTCGGAAAGTCTGGTGAATGAAATTTATCGCGGGGAAGACAGTATTGTCCGCCACTGGCTGAAAGCGCCGTGGAATATGGACGGCTGGCGGCTGGATGTGGTGCATATGCTGGGGGAGGCGGGTGGGGCGCGCAATAATATGCAGCACGt                                                                                                                >  2:564931/1‑150 (MQ=255)

                                        tCGTAAATTCTGGTTAATGAAATTTATCGCGGGGAAGACTGTATTGTCCGCCACTGGCTTAAAGCGCCTTGGAATTTGGTCGGGTGGCGGCTGGATGTGGTGCATATGCTGGGGGAGGCGGTTGGGGCGCGCAATAATATGCAGCACGtt                                                                                                               <  2:1840196/150‑1 (MQ=255)

                                        tCGGAATGTCTGGTGAATGAAATTTAGCGCGGGGAAGACAGTATTGCCCGCCACGGGCTGAAAGCGCGGTGGAATATGGCCGGCTGGCGGCTGGATGTGGTGCATATGCGGGGGGAGGCGGGTGGGGCGCGCAATAATATGCAGCACGtt                                                                                                               <  1:3047844/150‑1 (MQ=255)

                                         cGGAAAGTCTGGTGAATGATATTTATCGCGGGGAAGCCAGTATTGTCCGCCACGGGCTGAAAGCGCCGTGGAATAGGGACGGCTGGCGGCTGGATGTGGTGCATATGCTGGGGGAGGCGGGTGGGGCGCGCAATAAGATGCAGCACGTTg                                                                                                              <  2:2728965/150‑1 (MQ=255)

                                            aaaGTCTGGTGAATGAAATTTATCGCGGGGAAGACAGTATTGTCCGCCACTGGCTGAAAGCGCCGTGGAATATGGACGGCTGGCGGCTGGATGTGGTGCATATGCTGGGGGAGGCGGGTGGGGCGCGCAATAATATGCAGCACGTTGCCg                                                                                                           >  1:27238/1‑150 (MQ=255)

                                            aaaGTCTGGTGAATGAAATTTATCGCGGGGAAGACAGTATTGTCCGCCACTGGCTGAAAGCGCCGTGGAATATGGACGGCTGGCGGCTGGATGTGGTGCATATGCTGGGGGAGGCGGGTGGGGCGCGCAATAATATGCAGCACGTTGCCg                                                                                                           >  2:511927/1‑150 (MQ=255)

                                              aGTCTGGTGAATGAAATTTATCGCGGGGAAGACAGTATTGTCCGCCACTGGCTGAAAGCGCCGTGGAATATGGACGGCTGGCGGCTGGATGTGGTGCATATGCTGGGGGAGGCGGGTGGGGCGCGCAATAATATGCAGCACGTTGCCggg                                                                                                         >  1:1789772/1‑150 (MQ=255)

                                                tCTGGTGAATGAAATTTATCGCGGGGAAGACAGTATTGTCCGCCACTGGCTGAAAGCGCCGTGGAATATGGACGGCTGGCGGCTGGATGTGGTGCATATGCTGGGGGAGGCGGGTGGGGCGCGCAATAATATGCAGCACGTTGCCGGGAt                                                                                                       <  2:4395429/150‑1 (MQ=255)

                                                 cTGGTGAATGAAATTTATCGCGGGGAAGACAGTATTGTCCGCCACTGGCTGAAAGCGCCGTGGAATATGGACGGCTGGCGGCTGGATGTGGTGCATATGCTGGGGGAGGCGGGTGGGGCGCGCAATAATATGCAGCACGTTGCCGGGATc                                                                                                      >  1:2363398/1‑150 (MQ=255)

                                                   ggTGAATGAAATTTATCGCGGGGAAGACAGTATTGTCCGCCACTGGCTGAAAGCGCCGTGGAATATGGACGGCTGGCGGCTGGATGTGGTGCATATGCTGGGGGAGGCGGGTGGGGCGCGCAATAATATGCAGCACGTTGCCGGGATCAc                                                                                                    <  2:183910/150‑1 (MQ=255)

                                                   ggTGAATGAAATTTATCGCGGGGAAGACAGTATTGTCCGCCACTGGCTGAAAGCGCCGTGGAATATGGACGGCTGGCGGCTGGATGTGGTGCATATGCTGGGGGAGGCGGGTGGGGCGCGCAATAATATGCAGCACGTTGCCGGGATCAc                                                                                                    >  1:2006046/1‑150 (MQ=255)

                                                      gaatgaaATTTATCGCGGGGAAGACAGTATTGTCCGCCACTGGCTGAAAGCGCCGTGGAATATGGACGGCTGGCGGCTGGATGTGGTGCATATGCTGGGGGAGGCGGGTGGGGCGCGCACTAATATGCAGCACGTTGCCGGGATCACCGa                                                                                                 >  2:36226/1‑150 (MQ=255)

                                                        atgaaATTTATCGCGGGGAAGACAGTATTGTCCGCCACTGGCTGAAAGCGCCGTGGAATATGGACGGCTGGCGGCTGGATGTGGTGCATATGCTGGGGGAGGCGGGTGGGGCGCGCAATAATATGCAGCACGTTGCCGGGATCACCGAAg                                                                                               <  2:441761/150‑1 (MQ=255)

                                                        atgaaATTTATCGCGGGGAAGACAGTATTGTCCGCCACTGGCTGAAAGCGCCGTGGAATATGGACGGCTGGCGGCTGGATGTGGTGCATATGCTGGGGGAGGCGGGTGGGGCGCGCAATAATATGCAGCACGTTGCCGGGATCACCGAAg                                                                                               >  2:3759250/1‑150 (MQ=255)

                                                        atgaaATTTATCGCGGGGAAGACAGTATTGTCCGCCACTGGCTGAAAGCGCCGTGGAATATGGACGGCTGGCGGCTGGATGTGGTGCATATGCTGGGGGAGGCGGGTGGGGCGCGCAATAATATGCAGCACGTTGCCGGGATCACCGAAg                                                                                               >  2:793791/1‑150 (MQ=255)

                                                          gaaATTTAGCGCGGGGAAGACCGTATTGTCCGCCACGGGCTGAAAGCGCCGTGGAATATGGCCGGCTGGCGGCTGGATGTGGTGCATATGCGGGGGGAGGCGGGTGGGGCGCGCAATAATATGCAGCACGTTGCCGGGATCACCGAAgcg                                                                                             <  1:3894703/150‑1 (MQ=255)

                                                           aaaTTTATCGCGGGGAAGACAGTATTGTCCGCCACTGGCTGAAAGCGCCGTGGAATATGGACGGCTGGCGGCTGGATGTGGTGCATATGCTGGGGGAGGCGGGTGGGGCGCGCAATAATATGCAGCACGTTGCCGGGATCACCGAAgcgg                                                                                            >  2:344049/1‑150 (MQ=255)

                                                                tATCGCGGGGAAGACAGTATTGTCCGCCACTGGCTGAAAGCGCCGTGGAATATGGACGGCTGGCGGCTGGATGTGGTGCATATGCTGGGGGAGGCGGGTGGGGCGCGCAATAATATGCAGCACGTTGCCGGGATCACCGAAGCGGCgaaa                                                                                       >  1:3357266/1‑150 (MQ=255)

                                                                   cggggggAAGACTGTATTGTCCGCCACGGGCTTAACGCGCCGTGTAATATGGAGGGCTGGGGGGTGTTTGTGGTGAATATGCGGGGGGCGGGGGGTGGGGCGCGCAATAATATGCAGCACGTTGCCGGGATCACCGAAGCGGCgaaagaa                                                                                    <  1:403020/147‑1 (MQ=255)

                                                                     ggggtAAGACTGTTTTGGCCGCCTGTGGCTGAAAGCGCGGTGTATTTTGGCGGGCTGGGGGCTGGTTGGGGTGCATTTGGGGGGGGCGGGGGGTGGGGCGCGCAATAATATGCAGCACGTTGCCGGGATCACCGAAGCGGCGAAAGAAAc                                                                                  <  1:4162392/149‑1 (MQ=255)

                                                                       gggAAGACAGTATTGTCCGCCACTGGCTGAAAGCGCCGTGGAATATGGACGGCTGGCGGCTGGATGTGGTGCATATGCTGGGGGAGGCGGGTGGGGCGCGCAATAATATGCAGCACGTTGCCGGGATCACCGAAGCGGCGAAAGAAAccc                                                                                >  1:203831/1‑150 (MQ=255)

                                                                        ggAAGACAGTATTGTCCGCCACTGGCTGAAAGCGCCGTGGAATATGGACGGCTGGCGGCTGGATGTGGTGCATATGCTGGGGGAGGCGGGTGGGGCGCGCAATAATATGCAGCACGTTGCCGGGATCACCGAAGCGGCGAAAGAAACCCa                                                                               >  1:4266573/1‑150 (MQ=255)

                                                                            gACAGTATTGTCCGCCACTGGCTGAAAGCGCCGTGGAATATGGACGGCTGGCGGCTGGATGTGGTGCATATGCTGGGGGAGGCGGGTGGGGCGCGCAATAATATGCAGCACGTTGCCGGGATCACCGAAGCGGCGAAAGAAACCCAGCCg                                                                           >  1:1439374/1‑150 (MQ=255)

                                                                              cAGTATTGTCCGCCACTGGCTGAAAGCGCCGTGGAATATGGACGGCTGGCGGCTGGATGTGGTGCATATGCTGGGGGAGGCGGGTGGGGCGCGCAATAATATGCAGCACGTTGCCGGGATCACCGAAGCGGCGAAAGAAACCCAGCCGGa                                                                         <  2:4026213/150‑1 (MQ=255)

                                                                               aGTATTGTCCGCCACTGGCTGAAAGCGCCGTGGAATATGGACGGCTGGCGGCTGGATGTGGTGCATATGCTGGGGGAGGCGGGTGGGGCGCGCAATAATATGCAGCACGTTGCCGGGATCACCGAAGCGGCGAAAGAAACCCAGCCGGaa                                                                        >  2:308914/1‑150 (MQ=255)

                                                                                gTATTGTCCGCCACTGGCTGAAAGCGCCGTGGAATATGGACGGCTGGCGGCTGGATGTGGTGCATATGCTGGGGGAGGCGGGTGGGGCGCGCAATAATATGCAGCACGTTGCCGGGATCACCGAAGCGGCGAAAGAAACCCAGCCGGAAg                                                                       >  2:4475014/1‑150 (MQ=255)

                                                                                 tATTGTCCGCCACTGGCTGAAAGCGCCGTGGAATATGGACGGCTGGCGGCTGGATGTGGTGCATATGCTGGGGGAGGCGGGTGGGGCGCGCAATAATATGCAGCACGTTGCCGGGATCACCGAAGCGGCGAAAGAAACCCAGCCGGAAGc                                                                      >  1:3711025/1‑150 (MQ=255)

                                                                                   ttGTCCGCCACTGGCTGAAAGCGCCGTGGAATATGGACGGCTGGCGGCTGGATGTGGTGCATATGCGGGGGGAGGCGGGTGGGGCGCGCAATAATATGCAGCACGTTGCCGGGATCACCGAAGCGGCGAAAGAAACCCAGCCGGAAGCGt                                                                    <  2:4087366/150‑1 (MQ=255)

                                                                                      tCCGCCACTGGCTTAAAGCGCCGTGGAATATGGACGGCTGGCGGCTGGATGTGGTGCTTATGCTGGGGGTGGCGGGTGGGGCGCGCAATAATATGCAGCACGTTGCCGGGATCACCGAAGCGGCGAAAGAAACCCAGCCGGAAGCGtata                                                                 <  1:1845453/150‑1 (MQ=255)

                                                                                       ccGCCAGTGTCTGAAAGCGCCGTGGAATATGGAGGGCTGGGGGCTGGATGTGGTGCTTATGCGGGGGTAGGCGGGTGGGGCGCGCAATAATATGCAGCACGTTGCCGGGATCACCGAAGCGGCGAAAGAAACCCAGCCGGAAGCGTatat                                                                <  2:2584908/150‑1 (MQ=255)

                                                                                       ccGCCACTGGCTGAAAGCGCCGTGGAATATGGACGGCTGGCGGCTGGATGTGGTGCATATGCTGGGGGAGGCGGGTGGGGCGCGCAATAATATGCAGCACGTTGCCGGGATCACCGAAGCGGCGAAAGAAACCCAGCCGGAAGCGTatat                                                                >  2:2154849/1‑150 (MQ=255)

                                                                                        cGCCACTGGCTGAAAGCGCCGTGGAATATGGACGGCTGGCGGCTGGATGTGGTGCATATGCTGGGGGAGGCGGGTGGGGCGCGCAATAATATGCAGCACGTTGCCGGGATCACCGAAGCGGCGAAAGAAACCCAGCCGGAAGCGTATAtt                                                               >  1:1527987/1‑150 (MQ=255)

                                                                                          ccACTGGCTGAAAGCGCCGTGGAATATGGACGGCTGGCGGCTGGATGTGGTGCATATGCTGGGGGAGGCGGGTGGGGCGCGCAATAATATGCAGCACGTTGCCGGGATCACCGAAGCGGCGAAAGAAACCCAGCCGGAAGCGTATATTGt                                                             >  1:2666111/1‑150 (MQ=255)

                                                                                           cACTGGCTGAAAGGGCCGTGGAATTTGGACGGCTGGCGGCTGGATGTGGTGCATAGGGTGGGGGAGGGGGGTGGGGCGCGCAATAATATGCAGCACGTTGCCGGGATCACCGAAGCGGCGAAAGAAACCCAGCCGGAAGCGTATATTGTc                                                            <  1:823660/150‑1 (MQ=255)

                                                                                           cACGGGCTGAAAGCGCCGGGGAATAGGGACGGCGGGCGGCTGGATGGGGTGCATATGCGGGGGGCGGCGGGTGGGGCGCGCAATAATATGCAGCACGTTGCCGGGATCACCGAAGCGGCGAAAGAAACCCAGCCGGAAGCGTATATTGTc                                                            <  1:2454364/150‑1 (MQ=255)

                                                                                             cTGGCTGAAAGCGCCGTGGAATATGGACGGCTGGCGGCTGGATGTGGTGCATATGCTGGGGGAGGCGGGTGGGGCGCGCAATAATATGCAGCACGTTGCCGGGATCACCGAAGCGGCGAAAGAAACCCAGCCGGAAGCGTATATTGTCgg                                                          >  1:2245167/1‑150 (MQ=255)

                                                                                                  tGAAAGCGCCGTGGACTATGGACGGCTGGCGGCTGGATGTGGTGCATATGCTGGGGGAGGCGGGTGGGGCGCGCAATAATATGCAGCACGTTGCCGGGATCACCGAAGCGGCGAAAGAAACCCAGCCGGAAGCGTATATTGTCGGCGAAc                                                     >  1:1000288/1‑150 (MQ=255)

                                                                                                  tGAAAGCGCCGTGGAATATGGACGGCTGGCGGCTGGATGTGGTGCATATGCTGGGGGAGGCGGGTGGGGCGCGCAATAATATGCAGCACGTTGCCGGGATCACCGAAGCGGCGAAAGAAACCCAGCCGGAAGCGTATATTGTCGGCGAAc                                                     >  1:1199922/1‑150 (MQ=255)

                                                                                                  tGAAAGCGCCGTGGAATATGGACGGCTGGCGGCTGGATGTGGTGCATATGCTGGGGGAGGCGGGTGGGGCGCGCAATAATATGCAGCACGTTGCCGGGATCACCGAAGCGGCGAAAGAAACCCAGCCGGAAGCGTATATTGTCGGCGAAc                                                     >  1:3184474/1‑150 (MQ=255)

                                                                                                    aaaGCGCCGTGGAATATGGCCGGCTGGCGGCTGGTTGTGGTGCATTTGCGGGGGGAGGCGGGTGGGGCGCGCAATAATATGCAGCACGTTGCCGGGATCACCGAAGCGGCGAAAGAAACCCAGCCGGAAGCGTATATTGTCGGCGAACAt                                                   <  1:2771393/150‑1 (MQ=255)

                                                                                                       gcgcCGTGGAATATGGACGGCTGGCGGCTGGATGTGGTGCATATGCTGGGGGAGGCGGGTGGGGCGCGCAATAATATGCAGCACGTTGCCGGGATCACCGAAGCGGCGAAAGAAACCCAGCCGGAAGCGTATATTGTCGGCGAACAtttt                                                <  1:3867178/150‑1 (MQ=255)

                                                                                                        cgcCGTGGAATTTGGACGGCTGGCGGCTGGATGTGGTGCATATGCTGGGGGAGGCGGGTGGGGCGCGCAATAATATGCAGCACGTTGCCGGGATCACCGAAGCGGCGAAAGAAACCCAGCCGGAAGCGTATATTGTCGGCGAACATTTTg                                               <  2:2892347/150‑1 (MQ=255)

                                                                                                         gcCGTGGAATATGGACGGCTGGCGGCTGGATGTGGTGCATATGCTGGGGGAGGCGGGTGGGGCGCGCAATAATATGCAGCACGTTGCCGGGATCACCGAAGCGGCGAAAGAAACCCAGCCGGAAGCGTATATTGTCGGCGAACATTTTgg                                              >  2:3155319/1‑150 (MQ=255)

                                                                                                         gcCGTGGAATATGGACGGCTGGCGGCTGGATGTGGTGCATATGCTGGGGGAGGCGGGTGGGGCGCGCAATAATATGCAGCACGTTGCCGGGATCACCGAAGCGGCGAAAGAAACCCAGCCGGAAGCGTATATTGTCGGCGAACATTTTgg                                              <  1:3382723/150‑1 (MQ=255)

                                                                                                           cGTGGAATATGGACGGCTGGCGGCTGGATGTGGTGCATATGCTGGGGGAGGCGGGTGGGGCGCGCAATAATATGCAGCACGTTGCCGGGATCACCGAAGCGGCGAAAGAAACCCAGCCGGAAGCGTATATTGTCGGCGAACATTTTGGCg                                            >  2:1115467/1‑150 (MQ=255)

                                                                                                             tGGAATATGGACGGCTGGCGGCTGGATGTGGTGCATATGCGGGGGGAGGCGGGTGGGGCGCGCAATAATATGCAGCACGTTGCCGGGATCACCGAAGCGGCGAAAGAAACCCAGCCGGAAGCGTATATTGTCGGCGAACATTTTGGCGAt                                          <  1:4495416/150‑1 (MQ=255)

                                                                                                              ggAATTTGGACGGCGGGCGGGTGGATGTGGTGCATTTGCTGGGGGAGGCGGGTGGGGCGCGCAATAATATGCAGCACGTTGCCGGGATCACCGAAGCGGCGAAAGAAACCCAGCCGGAAGCGTATATTGTCGGCGAACATTTTGGCGATg                                         <  1:2678664/150‑1 (MQ=255)

                                                                                                               gAATATGGACGGCTGGCGGCTGGATGTGGTGCATAGGCTGGGGGAGGCGGGTGGGGCGCGCAATAATATGCAGCACGTTGCCGGGATCACCGAAGCGGCGAAAGAAACCCAGCCGGAAGCGTATATTGTCGGCGAACATTTTGGCGATGc                                        <  2:4211244/150‑1 (MQ=255)

                                                                                                                aaTATGGACGGCTGGCGGCTGGATGTGGTGCATATGCTGGGGGAGGCGGGTGGGGCGCGCAATAATATGCAGCACGTTGCCGGGATCACCGAAGCGGCGAAAGAAACCCAGCCGGAAGCGTATATTGTCGGCGAACATTTTGGCGATGCa                                       >  2:3133325/1‑150 (MQ=255)

                                                                                                                aaTATGGACGGCTGGCGGCTGGATGTGGTGCATATGCTGGGGGAGGCGGGTGGGGCGCGCAATAATATGCAGCACGTTGCCGGGATCACCGAAGCGGCGAAAGAAACCCAGCCGGAAGCGTATATTGTCGGCGAACATTTTGGCGATGCa                                       >  1:360227/1‑150 (MQ=255)

                                                                                                                 atatGGACGGCGGGCGGCTGGATGTGGTGCATATGCGGGGGGAGGCGGGTGGGGCGCGCAATAATATGCAGCACGTTGCCGGGATCACCGAAGCGGCGAAAGAAACCCAGCCGGAAGCGTATATTGTCGGCGAACATTTTGGCGATGCAc                                      <  2:233519/150‑1 (MQ=255)

                                                                                                                  tatGGACGGCTGGCGGCTGGATGTGGTGCATATGCTGGGGGAGGCGGGTGGGGCGCGCAATAATATGCAGCACGTTGCCGGGATCACCGAAGCGGCGAAAGAAACCCAGCCGGAAGCGTATATTGTCGGCGAACATTTTGGCGATGCACg                                     <  2:2218190/150‑1 (MQ=255)

                                                                                                                   atGGACGGCTGGCGGCTGGATGTGGTGCATATGCTGGGGGAGGCGGGTGGGGCGCGCAATAATATGCAGCACGTTGCCGGGATCACCGAAGCGGCGAAAGAAACCCAGCCGGAAGCGTATATTGTCGGCGAACATTTTGGCGATGCACgg                                    <  2:27238/150‑1 (MQ=255)

                                                                                                                     ggACGGCTGGCGGCTGGATGTGGTGCATATGCTGGGGGAGGCGGGTGGGGCGCGCAATAATATGCAGCACGTTGCCGGGATCACCGAAGCGGCGAAAGAAACCCAGCCGGAAGCGTATATTGTCGGCGAACATTTTGGCGATGCACGGCa                                  <  1:1625293/150‑1 (MQ=255)

                                                                                                                     ggACGGCTGGCGGCTGGATGTGGTGCATATGCGGGGGGAGGCGGGTGGGGCGCGCAATAATATGCAGCACGTTGCCGGGATCACCGAAGCGGCGAAAGAAACCCAGCCGGAAGCGTATATTGTCGGCGAACATTTTGGCGATGCACGGCa                                  >  1:3518792/1‑150 (MQ=255)

                                                                                                                        cggctggcggctggATGTGGTGCATATGCGGGGGGAGGCGGGTGGGGCGCGCAATAATATGCAGCACGTTGCCGGGATCACCGAAGCGGCGAAAGAAACCCAGCCGGAAGCGTATATTGTCGGCGAACATTTTGGCGATGCACGGCAATg                               <  2:121972/150‑1 (MQ=255)

                                                                                                                           ctggcggctggTTGTGGTGCATATGCTGGGGGAGGCGGGTGGGGCGCGCAATAATATGCAGCACGTTGCCGGGATCACCGAAGCGGCGAAAGAAACCCAGCCGGAAGCGTATATTGTCGGCGAACATTTTGGCGATGCACGGCAATGGtt                            <  1:2866829/150‑1 (MQ=255)

                                                                                                                                ggctggATGTGGTGCATATGCTGGGGGAGGCGGGTGGGGCGCGCAATAATATGCAGCACGTTGCCGGGATCACCGAAGCGGCGAAAGAAACCCAGCCGGAAGCGTATATTGTCGGCGAACATTTTGGCGATGCACGGCAATGGTTACAgg                       >  1:3586136/1‑150 (MQ=255)

                                                                                                                                 gctggATGTGGTGCATATGCTGGGGGAGGCGGGTGGGGCGCGCAATAATATGCAGCACGTTGCCGGGATCACCGAAGCGGCGAAAGAAACCCAGCCGGAAGCGTATATTGTCGGCGAACATTTTGGCGATGCACGGCAATGGTTACAGGc                      <  1:4086345/150‑1 (MQ=255)

                                                                                                                                   tggATGTGGTGCATATGCTGGGGGAGGCGGGTGGGGCGCGCAATAATATGCAGCACGTTGCCGGGATCACCGAAGCGGCGAAAGAAACCCAGCCGGAAGCGTATATTGTCGGCGAACATTTTGGCGATGCACGGCAATGGTTACAGGCCg                    >  2:1971116/1‑150 (MQ=255)

                                                                                                                                    ggATGTGGTGCATATGCTGGGGGAGGCGGGTGGGGCGCGCAATAATATGCAGCACGTTGCCGGGATCACCGAAGCGGCGAAAGAAACCCAGCCGGAAGCGTATATTGTCGGCGAACATTTTGGCGATGCACGGCAATGGTTACAGGCCGa                   >  1:3204767/1‑150 (MQ=255)

                                                                                                                                     gATGTGGTGCATATGCTGGGGGAGGCGGGTGGGGCGCGCAATAATATGCAGCACGTTGCCGGGATCACCGAAGCGGCGAAAGAAACCCAGCCGGAAGCGTATATTGTCGGCGAACATTTTGGCGATGCACGGCAATGGTTACAGGCCGAt                  >  1:4318804/1‑150 (MQ=255)

                                                                                                                                         tggtgCATATGCTGGGGGAGGCGGGTGGGGCGCGCAATAATATGCAGCACGTTGCCGGGATCACCGAAGCGGCGAAAGAAACCCAGCCGGAAGCGTATATTGTCGGCGAACATTTTGGCGATGCACGGCAATGGTTACAGGCCGATGTgg              <  1:1071667/150‑1 (MQ=255)

                                                                                                                                              cATATGCGGGGGGTGGCGGGTGGGGCGCGCAATAATATGCAGCACGTTGCCGGGATCACCGAAGCGGCGAAAGAAACCCAGCCGGAAGCGTATATTGTCGGCGAACATTTTGGCGATGCACGGCAATGGTTACAGGCCGATGTGGAAGAt         <  2:2582067/150‑1 (MQ=255)

                                                                                                                                               atatGCTGGGGGAGGCGGGTGGGGCGCGCAATAATATGCAGCACGTTGCCGGGATCACCGAAGCGGCGAAAGAAACCCAGCCGGAAGCGTATATTGTCGGCGAACATTTTGGCGATGCACGGCAATGGTTACAGGCCGATGTGGAAGATg        <  2:1573884/150‑1 (MQ=255)

                                                                                                                                                  tGCTGGGGGAGGCGGGTGGGGCGCGCAATAATATGCAGCACGTTGCCGGGATCACCGAAGCGGCGAAAGAAACCCAGCCGGAAGCGTATATTGTCGGCGAACATTTTGGCGATGCACGGCAATGGTTACAGGCCGATGTGGAAGATgccg     <  1:3928720/150‑1 (MQ=255)

CTGGCTTGGCTATGCCAGCTTGCCGAAGCTGGATTATCAGTCGGAAAGTCTGGTGAATGAAATTTATCGCGGGGAAGACAGTATTGTCCGCCACTGGCTGAAAGCGCCGTGGAATATGGACGGCTGGCGGCTGGATGTGGTGCATATGCTGGGGGAGGCGGGTGGGGCGCGCAATAATATGCAGCACGTTGCCGGGATCACCGAAGCGGCGAAAGAAACCCAGCCGGAAGCGTATATTGTCGGCGAACATTTTGGCGATGCACGGCAATGGTTACAGGCCGATGTGGAAGATGCCGCCA  >  CP009273/418846‑419144

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`