Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 49,840 G→T 80.0% A6A (GCG→GCT)  folA → dihydrofolate reductase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP00927349,8400GT80.0% 472.8 / 147.2 225A6A (GCG→GCT) folAdihydrofolate reductase
Reads supporting (aligned to +/- strand):  ref base G (21/24);  new base T (93/86);  total (115/110)
Fisher's exact test for biased strand distribution p-value = 6.17e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.80e-01

Only 100 of 230 total aligned reads displayed.

GCGAGTCCAGGGAGAGAGCGTGGACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTCGACGACGGTTTACGCTTTACGTATAGTGGCGACAA‑TTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCTGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATACCTGGGAATCAATCGGTCGTCCGTTGCC  >  CP009273/49693‑49986
                                                                                                                                                    |                                                                                                                                                  
  gAGTCCAGGGAGAGAGCGTGGACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTCGACGACGGTTTACGCTTTACGTATAGTGGCGACAA‑TTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCTGCGt                                                                                                                                                <  2:2970823/150‑1 (MQ=255)
   aGTCCAGGGAGAGAGCGTGGACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTCGACGACGGTTTACGCTTTACGTATAGTGGCGACAA‑TTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCGGCGtt                                                                                                                                               <  1:353601/150‑1 (MQ=255)
     tCCAGGGAGAGAGCGTGGACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTCGACGACGGTTTACGCTTTACGTATAGTGGCGACAA‑TTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCTGCGTTAg                                                                                                                                             >  1:4164923/1‑150 (MQ=255)
      ccAGGGAGAGAGCGTGGACTCGCCAGCAGAATATAAACTTTTCCTCAACATCATCCTCGCACCAGTCGACGACGGTTTACGCTTTACGTATAGTGGCGACAA‑TTTTTTTTATCGGGAAATCTCACTGATCAGTCTGATTGCTGCGTTAGc                                                                                                                                            <  1:269159/150‑1 (MQ=255)
       cAGGGAGAGAGCGTGGACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTCGACGACGGTTTACGCTTTACGTATAGTGGCGACAA‑TTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCTGCGTTAGCg                                                                                                                                           <  1:1692800/150‑1 (MQ=255)
         gggAGAGAGCGTGGACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTCGACGACGGTTTACGCTTTACGTATAGTGGCGACAA‑TTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCTGCGTTAGCGGt                                                                                                                                         >  1:2191099/1‑150 (MQ=255)
                agCGTGGACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTCGACGACGGTTTACGCTTTACGTATAGTGGCGACAA‑TTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCTGCGTTAGCGGTAGATcgc                                                                                                                                  <  2:296786/150‑1 (MQ=255)
                agCGTGGACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTCGACGACGGTTTACGCTTTACGTATAGTGGCGACAA‑TTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCTGCGTTAGCGGTAGATcgc                                                                                                                                  <  2:4143833/150‑1 (MQ=255)
                agCGTGGACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTCGACGACGGTTTACGCTTTACGTATAGTGGCGACAA‑TTTTTTTTATCGGGAAATCTCAATGATCAGTCTGAGTGCGGCGTTAGCGGTAGATcgc                                                                                                                                  >  2:3048709/1‑150 (MQ=255)
                  cGTGGACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTCGACGACGGTTTACGCTTTACGTATAGTGGCGACAA‑TTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCTGCGTTAGCGGTAGATCGCGt                                                                                                                                <  2:3310107/150‑1 (MQ=255)
                    tGGACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTCGACGACGGTTTACGCTTTACGTATAGTGGCGACAA‑TTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTa                                                                                                                              >  1:1369075/1‑150 (MQ=255)
                      gACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTCGACGACGGTTTACGCTTTACGTATAGTGGCGACAA‑TTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATc                                                                                                                            <  2:429943/150‑1 (MQ=255)
                      gACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTCGACGACGGTTTACGCTTTACGTATAGTGGCGACAA‑TTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATc                                                                                                                            <  2:431546/150‑1 (MQ=255)
                            ccAGCAGAATATAAAATTTTCCTCAACATCCTCCTCGCACCAGTCGACGACGGTTTACGCTTTACGTATAGTGGCGACAA‑TTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCTGCGTTAGCGGTAGATCGCGTTATCGGCATg                                                                                                                      <  2:2735807/150‑1 (MQ=255)
                              agcagAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTCGACGACGGTTTACGCTTTACGTATAGTGGCGACAA‑TTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCTGCGTTAGCGGTAGATCGCGTTATCGGCATGGa                                                                                                                    >  1:2632246/1‑150 (MQ=255)
                               gcagAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTCGACGACGGTTTACGCTTTACGTATAGTGGCGACAA‑TTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCTGCGTTAGCGGTAGATCGCGTTATCGGCATGGaa                                                                                                                   >  1:1513137/1‑150 (MQ=255)
                                  gAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTCGACGACGGTTTACGCTTTACGTATAGTGGCGACAA‑TTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCTGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAAc                                                                                                                <  2:368034/150‑1 (MQ=255)
                                  gAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTCGACGACGGTTTACGCTTTACGTATAGTGGCGACAA‑TTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCTGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAAc                                                                                                                <  2:2496048/150‑1 (MQ=255)
                                  gAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTCGACGACGGTTTACGCTTTACGTATAGTGGCGACAA‑TTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCTGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAAc                                                                                                                <  1:2527888/150‑1 (MQ=255)
                                        aaaaTTTTCCTCAACATCATCCTCGCACCAGTCGACGACGGTTTACGCTTTACGTATAGTGGCGACAATTTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCTGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCAt                                                                                                           <  1:1957275/150‑1 (MQ=255)
                                         aaaTTTTCCTCAACATCATCCTCGCACCAGTCGACGACGGTTTACGCTTTACGTATAGTGGCGACAA‑TTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCTGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGc                                                                                                         >  2:4352898/1‑150 (MQ=255)
                                          aaTTTTCCTCAACATCATCCTCGCACCAGTCGACGACGGTTTACGCTTTACGTATAGTGGCGACAA‑TTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCTGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGcc                                                                                                        <  2:228535/150‑1 (MQ=255)
                                            ttttCCTCAACATCATCCTCGCACCAGTCGACGACGGTTTACGCTTTACGTATAGTGGCGACAA‑TTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGt                                                                                                      >  2:362117/1‑150 (MQ=255)
                                               tCCTCAACATCATCCTCGCACCAGTCGACGACGGTTTACGCTTTACGTATAGTGGCGACAA‑TTTTTTTTATCGGGAAATCTCCATGATCAGTCTGATTGCTGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGa                                                                                                   <  1:3725173/150‑1 (MQ=255)
                                               tCCTCAACATCATCCTCGCACCAGTCGACGACGGTTTACGCTTTACGTATAGTGGCGACAA‑TTTTTTTTATCGGGAAATCTCACTGATCAGTCTGATTGCTGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGa                                                                                                   <  1:4519484/150‑1 (MQ=255)
                                               tCCTCAACATCATCCTCGCACCAGTCGACGACGGTTTACGCTTTACGTATAGTGGCGACAA‑TTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCTGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGa                                                                                                   <  1:1172023/150‑1 (MQ=255)
                                                ccTCAACATCATCCTCGCACCAGTCGACGACGGTTTACGCTTTACGTATAGTGGCGACAA‑TTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCTGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGaa                                                                                                  <  1:565543/150‑1 (MQ=255)
                                                ccTCAACATCATCCTCGCACCAGTCGACGACGGTTTACGCTTTACGTATAGTGGCGACAA‑TTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGaa                                                                                                  <  1:178883/150‑1 (MQ=255)
                                                 cTCAACATCATCCTCGCACCAGTCGACGACGGTTTACGCTTTACGTATAGTGGCGACAA‑TTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCTGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAAc                                                                                                 >  1:2912311/1‑150 (MQ=255)
                                                    aaCATCATCCTCGCACCAGTCGACGACGGTTTACGCTTTACGTATAGTGGCGACAA‑TTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCTGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAAcctg                                                                                              >  2:78819/1‑150 (MQ=255)
                                                    aaCATCATCCTCGCACCAGTCGACGACGGTTTACGCTTTACGTATAGTGGCGACAA‑TTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCTGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAAcctg                                                                                              >  1:4139513/1‑150 (MQ=255)
                                                    aaCATCATCCTCGCACCAGTCGACGACGGTTTACGCTTTACGTATAGTGGCGACAA‑TTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAAcctg                                                                                              >  2:383182/1‑150 (MQ=255)
                                                      catcatCCTCGCACCAGTCGACGACGGTTTACGCTTTACGTATAGTGGCGACAA‑TTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCTGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAAcctgcc                                                                                            >  2:1204050/1‑150 (MQ=255)
                                                       atcatcCTCGCACCAGTCGACGACGGTTTACGCTTTACGTATAGTGGCGACAA‑TTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCTGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAAcctgcct                                                                                           >  2:208899/1‑150 (MQ=255)
                                                       atcatcCTCGCACCAGTCGACGACGGTTTACGCTTTACGTATAGTGGCGACAA‑TTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCTGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAAcctgcct                                                                                           <  1:2383661/150‑1 (MQ=255)
                                                       atcagcCTCGCACCAGTCGACGACGGTTTACGCTTTACGTATAGTGGCGACAA‑TTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCTGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAAcctgcct                                                                                           <  1:3214179/150‑1 (MQ=255)
                                                         catcCTCGCACCAGTCGACGACGGTTTACGCTTTACGTATAGTGGCGACAA‑TTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCTGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACctgcctgc                                                                                         >  2:2585312/1‑150 (MQ=255)
                                                          atcCTCGCACCAGTCGACGACGGTTTACGCTTTACGTATAGTGGCGACAA‑TTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCTGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCtgcctgcc                                                                                        <  2:226096/150‑1 (MQ=255)
                                                          atcCTCGCACCAGTCGACGACGGTTTACGCTTTACGTATAGTGGCGACAA‑TTTTTTTTATCGGGAAATCTCAATGATCAGTATGATTGCTGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGAGGAACCtgcctgcc                                                                                        >  1:601982/1‑150 (MQ=255)
                                                          atcCTCGCACCAGTCGACGACGGTTTACGCTTTACGTATAGTGGCGACAA‑TTTTGTTTATCGGGAAATCTCAATGATCAGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCtgcctgcc                                                                                        >  2:2161288/1‑150 (MQ=255)
                                                              tCGCACCAGTCGACGACGGTTTACGCTTTACGTATAGTGGCGACAA‑TTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCTGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGAtc                                                                                    <  1:3355115/150‑1 (MQ=255)
                                                               cGCACCAGTCGACGACGGTTTACGCTTTACGTATAGTGGCGACAA‑TTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCTGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGAtct                                                                                   >  2:868166/1‑150 (MQ=255)
                                                                gCACCAGTCGACGACGGTTTACGCTTTACGTATAGTGGCGACAA‑TTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCTGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGAtctc                                                                                  >  1:2536170/1‑150 (MQ=255)
                                                                 cACCAGTCGACGACGGTTTACGCTTTACGTATAGTGGCGACAA‑TTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCTGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCg                                                                                 >  1:3793415/1‑150 (MQ=255)
                                                                      gTCGACGACGGTTTACGCTTTACGTATAGTGGCGACAA‑TTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCTGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCTgg                                                                            <  1:3873843/150‑1 (MQ=255)
                                                                         gacgacGGTTTACGCTTTACGTATAGTGGCGACAA‑TTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCTGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCTGGttt                                                                         >  2:1106091/1‑150 (MQ=255)
                                                                           cgacgGTTTACGCTTTACGTATAGTGGCGACAA‑TTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCTGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCTGGTTTaa                                                                       <  1:2737010/150‑1 (MQ=255)
                                                                           cgacgGTTTACGCTTTACGTATAGTGGCGACAA‑TTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCTGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCTGGTTTaa                                                                       <  2:3673180/150‑1 (MQ=255)
                                                                           cgacgGTTTACGCTTTACGTATAGTGGCGACAA‑TTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCTGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCTGGTTTaa                                                                       <  2:1035198/150‑1 (MQ=255)
                                                                           cgacgGTTTACGCTTTACGTATAGTGGCGACAA‑TTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCTGGTTTaa                                                                       <  1:37276/150‑1 (MQ=255)
                                                                              cgGTTTACGCATTACGTATAGTGGCGACAA‑TTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCTGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCTGGTTTAAACg                                                                    <  2:2001468/150‑1 (MQ=255)
                                                                                gTTTACGCTTTACGTATAGTGGCGACAA‑TTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCTGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCTGGTTTAAACGCa                                                                  <  2:696400/150‑1 (MQ=255)
                                                                                gTTTACGCTTTACGTATAGTGGCGACAA‑TTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCTGGTTTAAACGCa                                                                  >  2:1676964/1‑150 (MQ=255)
                                                                                      gCTTTACGTATAGTGGCGACAA‑TTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCTGGTTTAAACGCAACACCt                                                            <  1:3313775/150‑1 (MQ=255)
                                                                                       cTTTACGTATAGTGGCGACAA‑TTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCTGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCTGGTTTAAACGCAACACCtt                                                           >  2:1421094/1‑150 (MQ=255)
                                                                                       cTTTACGTATAGTGGCGACAA‑TTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCTGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCTGGTTTAAACGCAACACCtt                                                           >  2:4522982/1‑150 (MQ=255)
                                                                                        tttACGTATAGTGGCGACAA‑TTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCTGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCTGGTTTAAACGCAACACCTta                                                          <  2:671477/150‑1 (MQ=255)
                                                                                        tttACGTATAGTGGCGACAA‑TTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCTGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCTGGTTTAAACGCAACACCTta                                                          <  2:2978204/150‑1 (MQ=255)
                                                                                         ttACGTATAGTGGCGACAA‑TTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCTGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCTGGTTTAAACGCAACACCTtaa                                                         >  1:1622400/1‑150 (MQ=255)
                                                                                         ttACGTATAGTGGCGACAA‑TTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCTGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCTGGTTTAAACGCAACACCTtaa                                                         >  1:1949028/1‑150 (MQ=255)
                                                                                          tACGTATAGTGGCGACAA‑TTTTTTTTATCGGGAAATCTCAATGATGTGTCTGATTGCTGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCTGGTTTAAACGCAACACCTtaaa                                                        <  1:1183704/150‑1 (MQ=255)
                                                                                           aCGTATAGTGGCGACAA‑TTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCTGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCTGGTTTAAACGCAACACCTtaaat                                                       >  2:467339/1‑150 (MQ=255)
                                                                                           aCGTATAGTGGCGACAA‑TTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCTGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCTGGTTTAAACGCAACACCTtaaat                                                       <  1:1759859/150‑1 (MQ=255)
                                                                                           aCGTATAGTGGCGACAA‑TTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCTGGTTTAAACGCAACACCTtaaat                                                       <  1:846652/150‑1 (MQ=255)
                                                                                            cGTATAGTGGCGACAA‑TTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCTGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCTGGTTTAAACGCAACACCTtaaata                                                      <  2:2216630/150‑1 (MQ=255)
                                                                                             gTATAGTGGCGACAA‑TTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCTGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCTGGTTTAAACGCAACACCTtaaataa                                                     >  2:547851/1‑150 (MQ=255)
                                                                                             gTATAGTGGCGACAA‑TTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCTGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCTGGTTTAAACGCAACACCTtaaataa                                                     <  1:3624447/150‑1 (MQ=255)
                                                                                             gTATAGTGGCGACAA‑TTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCTGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCTGGTGTAAACGCAACACCTtaaataa                                                     >  2:3757159/1‑150 (MQ=255)
                                                                                               ataGTGGCGACAA‑TTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCTGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCTGGTTTAAACGCAACACCTTAAATAAAc                                                   <  2:3459017/150‑1 (MQ=255)
                                                                                                 aGTGGCGACAA‑TTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCTGGTTTAAACGCAACACCTTAAATAAAccc                                                 >  2:4326693/1‑150 (MQ=255)
                                                                                                  gTGGCGACAA‑TTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCTGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCTGGTTTAAACGCAACACCTTAAATAAACCCg                                                <  1:3522703/150‑1 (MQ=255)
                                                                                                   tGGCGACAA‑TTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCTGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCTGGTTTAAACGCAACACCTTAAATAAACCCGt                                               >  1:944450/1‑150 (MQ=255)
                                                                                                      cGACAA‑TTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCTGGTTTAAACGCAACACCTTAAATAAACCCGTGAt                                            <  2:4044686/150‑1 (MQ=255)
                                                                                                        aCAA‑TTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCTGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCTGGTTTAAACGCAACACCTTAAATAAACCCGTGATTa                                          >  1:822754/1‑150 (MQ=255)
                                                                                                         cAA‑TTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCTGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCTGGTTTAAACGCAACACCTTAAATAAACCCGTGAttct                                         >  2:3094694/1‑148 (MQ=255)
                                                                                                         cAA‑TTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCTGGTTTAAACGCAACACCTTAAATAAACCCGTGATTAt                                         <  1:383182/150‑1 (MQ=255)
                                                                                                          aa‑TTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCTGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCTGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATg                                        >  2:2321042/1‑150 (MQ=255)
                                                                                                          aa‑TTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCTGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCTGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATg                                        <  1:150223/150‑1 (MQ=255)
                                                                                                                tttttATCGGGAAATCTCAATGATCAGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGAGGAACCTGCCTGCCGATCTCGCCTGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGgccg                                   >  1:4237668/1‑150 (MQ=255)
                                                                                                                  tttATCGGGAAATCTCAATGATCAGTCTGATTGCTGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCTGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGgccgcc                                 <  2:20421/150‑1 (MQ=255)
                                                                                                                       cGGGAAATCTCAATGATCAGTCTGATTGCTGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCTGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATAcc                            >  2:635531/1‑150 (MQ=255)
                                                                                                                        gggAAATCTCAATGATCAGTCTGATTGCTGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCTGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATACCt                           >  2:2932743/1‑150 (MQ=255)
                                                                                                                        gggAAATCTCAATGATCAGTCTGATTGCTGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCTGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATACCt                           >  1:230520/1‑150 (MQ=255)
                                                                                                                        gggAAATCTCAATGATCAGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCTGGTTTAAACTCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATACCt                           <  1:1019609/150‑1 (MQ=255)
                                                                                                                        gggAAATCTCAATGATCAGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCTGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATACCt                           <  1:1018848/150‑1 (MQ=255)
                                                                                                                        gggAAATCTCAAAGATCAGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCTGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATACCt                           <  1:3921206/150‑1 (MQ=255)
                                                                                                                           aaaTCTCAATGATCAGTCTGATTGCCGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCTGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATACCTggg                        >  1:4068981/1‑150 (MQ=255)
                                                                                                                            aaTCTCAATGATCAGTCTGATTGCTGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCTGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATACCTGGGa                       >  2:1726211/1‑150 (MQ=255)
                                                                                                                            aaTCTCAATGATCAGTCTGATTGCTGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCTGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATACCTGGGa                       >  2:4535499/1‑150 (MQ=255)
                                                                                                                                tcAATGATCAGTCTGATTGCTGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCTGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATACCTGGGaatca                   >  2:3161206/1‑150 (MQ=255)
                                                                                                                                   aTGATCAGTCTGATTGCTGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCTGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATACCTGGGactcaatc                >  1:4321442/1‑150 (MQ=255)
                                                                                                                                   aTGATCAGTCTGATTGCTGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCTGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATACCTGGGaatcaatc                >  1:3288157/1‑150 (MQ=255)
                                                                                                                                   aTGATCAGTCTGATTGCTGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCTGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATACCTGGGaatcaatc                >  1:4323757/1‑150 (MQ=255)
                                                                                                                                       tCAGTCTGATTGCTGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCTGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATACCTGGGAATCAATCGgtc            <  1:683976/150‑1 (MQ=255)
                                                                                                                                           tCTGATTGCTGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCTGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATACCTGGGAATCAATCGGTCGTcc        <  1:1683410/150‑1 (MQ=255)
                                                                                                                                           tCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCTGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATACCTGGGAATCAATCGGTCGTcc        <  2:1689801/150‑1 (MQ=255)
                                                                                                                                            cTGATTGCTGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCTGGTTTAAACGCACCACCTTAAATAAACCCGTGATTATGGGCCGCCATACCTGGGAATCAATCGGTCGTCCg       >  2:1344828/1‑150 (MQ=255)
                                                                                                                                            cTGATTGCTGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCTGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATACCTGGGAATCAATCGGTCGTCCg       >  1:2990242/1‑150 (MQ=255)
                                                                                                                                             tGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCTGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATACCTGGGAATCAATCGGTCGTCCGt      >  1:4361410/1‑150 (MQ=255)
                                                                                                                                               aTTGCTGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCTGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATACCTGGGAATCAATCGGTCGTCCGTTg    >  2:2377539/1‑150 (MQ=255)
                                                                                                                                                    |                                                                                                                                                  
GCGAGTCCAGGGAGAGAGCGTGGACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTCGACGACGGTTTACGCTTTACGTATAGTGGCGACAA‑TTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCTGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATACCTGGGAATCAATCGGTCGTCCGTTGCC  >  CP009273/49693‑49986

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑