Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 441,811 A→C 14.5% I104M (ATT→ATG)  yajR ← putative transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273441,8110AC14.5% 663.7 / 111.2 261I104M (ATT→ATG) yajRputative transporter
Reads supporting (aligned to +/- strand):  ref base A (129/94);  new base C (16/22);  total (145/116)
Fisher's exact test for biased strand distribution p-value = 7.92e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.98e-01

Only 100 of 273 total aligned reads displayed.

ACCATCGCAATGGCAAAGGTAATGCCAAAGCTCACGCCGATAAACGCCATCGCTTTGGTGCGGTTTTGTTCGCGCGTGAGATCGGAAAGCAGCGCCATAACGGCGGCGGCAATTGCTCCAGAGCCTTGTAGCGCCCGGCCCAGAATAATTCCCCAGATGGAGTCGGAAAGCGCAGCGATAACGCTACCGGCGGCAAACACCGCCAGCCCACCGACAATTAATGGTTTGCGACCAATGCGGTCGGAAAGCAGGCCAAACGGAATCTGAAAAACGGCCTGAGTCAGACCATAAATACC  >  CP009273/441665‑441960
                                                                                                                                                  |                                                                                                                                                     
  cATCGCAATGGCAAAGGTAATGCCAAAGCTCACGCCGATAAACGCCATCGCTTTGGTGCGGTTTTGTTCGCGCGTGAGATCGGAAAGCAGCGCCATAACGGCGGCGGCAATTGCTCCAGAGCCTTGTAGCGCCCGGCCCAGAATAATTcc                                                                                                                                                  >  2:1676956/1‑150 (MQ=255)
     cGCAATGGCAAAGGTAATGCCAAAGCTCACGCCGATAAACGCCATCGCTTTGGTGCGGTTTTGTTCGCGCGTGAGATCGGAAAGCAGCGCCATAACGGCGGCGGCAATTGCTCCAGAGCCTTGTAGCGCCCGGCCCAGAATAATTCCCCa                                                                                                                                               <  2:3322325/150‑1 (MQ=255)
        aaTGGCAAAGGTAATGCCAAAGCTCACGCCGATAAACGCCATCGCTTTGGTGCGGTTTTGTTCGCGCGTGAGATCGGAAAGCAGCGCCATAACGGCGGCGGCAATTGCTCCAGAGCCTTGTAGCGCCCGGCCCAGAATAATTCCCCAGAt                                                                                                                                            <  1:1812096/150‑1 (MQ=255)
         aTGGCAAAGGTAATGCCAAAGCTCACGCCGATAAACGCCATCGCTTTGGTGCGGTTTTGTTCGCGCGTGAGATCGGAAAGCAGCGCCATAACGGCGGCGGCAATTGCTCCAGAGCCTTGTAGCGCCCGGCCCAGAATCATTCCCCAGATg                                                                                                                                           <  2:3436566/150‑1 (MQ=255)
          tGGCAAAGGTAATGCCAAAGCTCACGCCGATAAACGCCATCGCTTTGGTGCGGTTTTGTTCGCGCGTGAGATCGGAAAGCAGCGCCATAACGGCGGCGGCACTTGCTCCAGAGCCTTGTAGCGCCCGGCCCAGAATAATTCCCCAGATgg                                                                                                                                          >  2:3560945/1‑150 (MQ=255)
             cAAAGGTAATGCCAAAGCTCACGCCGATAAACGCCATCGCTTTGGTGCGGTTTTGTTCGCGCGTGAGATCGGAAAGCAGCGCCATAACGGCGGCGGCAATTGCTCCAGAGCCTTGTAGCGCCCGGCCCAGAATCATTCCCCAGATGGAGt                                                                                                                                       >  2:4173825/1‑150 (MQ=255)
              aaaGGTAATGCCAAAGCTCACGCCGATAAACGCCATCGCTTTGGTGCGGTTTTGTTCGCGCGTGAGATCGGAAAGCAGCGCCATAACGGCGGCGGCAATTGCTCCAGAGCCTTGTAGCGCCCGGCCCAGAATAATTCCCCAGATGGAGTc                                                                                                                                      <  1:2927487/150‑1 (MQ=255)
               aaGGTAATGCCAAAGCTCACGCCGATAAACGCCATCGCTTTGGTGCGGTTTTGTTCGCGCGTGAGATCGGAAAGCAGCGCCATAACGGCGGCGGCAATTGCTCCAGAGCCTTGTAGCGCCCGGCCCAGAATAATTCCCCAGATGGAGTCg                                                                                                                                     >  1:3719065/1‑150 (MQ=255)
                aGGTAATGCCAAAGCTCACGCCGATAAACGCCATCGCTTTGGTGCGGTTTTGTTCGCGCGTGAGATCGGAAAGCAGCGCCATAACGGCGGCGGCAATTGCTCCAGAGCCTTGTAGCGCCCGGCCCAGAATAATTCCCCAGATGGAGTCgg                                                                                                                                    >  2:3725648/1‑150 (MQ=255)
                 ggTAATGCCAAAGCTCACGCCGATAAACGCCATCGCTTTGGTGCGGTTTTGTTCGCGCGTGAGATCGGAAAGCAGCGCCATAACGGCGGCGGCAATTGCTCCAGAGCCTTGTAGCGCCCGGCCCAGACTAATTCCCCAGATGGAGTCGGa                                                                                                                                   >  2:3791600/1‑150 (MQ=255)
                    aaTGCCAAAGCTCACGCCGATAAACGCCATCGCTTTGGTGCGGTTTTGTTCGCGCGTGAGATCGGAAAGCAGCGCCATAACGGCGGCGGCAATTGCTCCAGAGCCTTGTAGCGCCCGGCCCAGAATAATTCCCCAGATGGAGTCGGAAAg                                                                                                                                >  2:4424477/1‑150 (MQ=255)
                      tGCCAAAGCTCACGCCGATAAACGCCATCGCTTTGGTGCGGTTTTGTTCGCGCGTGAGATCGGAAAGCAGCGCCATAACGGCGGCGGCAATTGCTCCAGAGCCTTGTAGCGCCCGGCCCAGAATAATTCCCCAGATGGAGTCGGAAAgcg                                                                                                                              >  2:599551/1‑150 (MQ=255)
                        ccAAAGCTCACGCCGATAAACGCCATCGCTTTGGTGCGGTTTTGTTCGCGCGTGAGATCGGAAAGCAGCGCCATAACGGCGGCGGCAATTGCTCCAGAGCCTTGTAGCGCCCGGCCCAGAATAATTCCCCAGATGGAGTCGGAAAGCGCa                                                                                                                            >  2:2764160/1‑150 (MQ=255)
                         cAAAGCTCACGCCGATAAACGCCATCGCTTTGGTGCGGTTTTGTTCGCGCGTGAGATCGGAAAGCAGCGCCATAACGGCGGCGGCAATTGCTCCAGAGCCTTGTAGCGCCCGGCCCAGAATAATTCCCCAGATGGAGTCGGAAAGCGCAg                                                                                                                           >  2:4143956/1‑150 (MQ=255)
                           aaGCTCACGCCGATAAACGCCATCGCTTTGGTGCGGTTTTGTTCGCGCGTGAGATCGGAAAGCAGCGCCATAACGGCGGCGGCAATTGCTCCAGAGCCTTGTAGCGCCCGGCCCAGAATAATTCCCCAGATGGAGTCGGAAAGCGCAGCg                                                                                                                         <  1:2537279/150‑1 (MQ=255)
                           aaGCTCACGCCGATAAACGCCATCGCTTTGGTGCGGTTTTGTTCGCGCGTGAGATCGGAAAGCAGCGCCATAACGGCGGCGGCAATTGCTCCAGAGCCTTGTAGCGCCCGGCCCAGAATAATTCCCCAGATGGAGTCGGAAAGCGCAGCg                                                                                                                         >  1:4246204/1‑150 (MQ=255)
                           aaGCTCACGCCGATAAACGCCATCGCTTTGGTGCGGTTTTGTTCGCGCGTGAGATCGGAAAGCAGCGCCATAACGGCGGCGGCAATTGCTCCAGAGCCTTGTAGCGCCCGGCCCAGAATAATTCCCCAGATGGAGTCGGAAAGCGCAGCg                                                                                                                         >  1:543272/1‑150 (MQ=255)
                           aaGCTCACGCCGATAAACGCCATCGCTTTGGTGCGGTTTTGTTCGCGCGTGAGATCGGAAAGCAGCGCCATAACGGCGGCGGCAATTGCTCCAGAGCCTTGTAGCGCCCGGCCCAGAATAATTCCCCAGATGGAGTCGGAAAGCGCAGCg                                                                                                                         >  2:1934608/1‑150 (MQ=255)
                              cTCACGCCGATAAACGCCATCGCTTTGGTGCGGTTTTGTTCGCGCGTGAGATCGGAAAGCAGCGCCATAACGGCGGCGGCAATTGCTCCAGAGCCTTGTAGCGCCCGGCCCAGAATAATTCCCCAGATGGAGTCGGAAAGCGCAGCGATa                                                                                                                      <  1:4146260/150‑1 (MQ=255)
                                cACGCCGATAAACGCCATCGCTTTGGTGCGGTTTTGTTCGCGCGTGAGATCGGAAAGCAGCGCCATAACGGCGGCGGCAATTGCTCCAGAGCCTTGTAGCGCCCGGCCCAGAATAATTCCCCAGATGGAGTCGGAAAGCGCAGCGATAAc                                                                                                                    <  1:3939043/150‑1 (MQ=255)
                                   gCCGATAAACGCCATCGCTTTGGTGCGGTTTTGTTCGCGCGTGAGATCGGAAAGCAGCGCCATAACGGCGGCGGCAATTGCTCCAGAGCCTTGTAGCGCCCGGCCCAGAATCATTCCCCAGATGGAGTCGGAAAGCGCAGCGATAACGCt                                                                                                                 <  1:1191507/150‑1 (MQ=255)
                                    ccGATAAACGCCATCGCTTTGGTGCGGTTTTGTTCGCGCGTGAGATCGGAAAGCAGCGCCATAACGGCGGCGGCAATTGCTCCAGAGCCTTGTAGCGCCCGGCCCAGAATAATTCCCCAGATGGAGTCGGAAAGCGCAGCGATAACGCTa                                                                                                                <  2:2696925/150‑1 (MQ=255)
                                    ccGATAAACGCCATCGCTTTGGTGCGGTTTTGTTCGCGCGTGAGATCGGAAAGCAGCGCCATAACGGCGGCGGCAATTGCTCCAGAGCCTTGTAGCGCCCGGCCCAGAATAATTCCCCAGATGGAGTCGGAAAGCGCAGCGATAACGCTa                                                                                                                <  1:3315773/150‑1 (MQ=255)
                                     cGATAAACGCCATCGCTTTGGTGCGGTTTTGTTCGCGCGTGAGATCGGAAAGCAGCGCCATAACGGCGGCGGCAATTGCTCCAGAGCCTTGTAGCGCCCGGCCCAGAATAATTCCCCAGATGGAGTCGGAAAGCGCAGCGATAACGCTAc                                                                                                               <  1:1283457/150‑1 (MQ=255)
                                      gATAAACGCCATCGCTTTGGTGCGGTTTTGTTCGCGCGTGAGATCGGAAAGCAGCGCCATAACGGCGGCGGCAATTGCTCCAGAGCCTTGTAGCGCCCGGCCCAGAATAATTCCCCAGATGGAGTCGGAAAGCGCAGCGATAACGCTAcc                                                                                                              >  2:7173/1‑150 (MQ=255)
                                      gATAAACGCCATCGCTTTGGTGCGGTTTTGTTCGCGCGTGAGATCGGAAAGCAGCGCCATAACGGCGGCGGCAATTGCTCCAGAGCCTTGTAGCGCCCGGCCCAGAATAATTCCCCAGATGGAGTCGGAAAGCGCAGCGATAACGCTAcc                                                                                                              >  2:6167/1‑150 (MQ=255)
                                        tAAACGCCATCGCTTTGGTGCGGTTTTGTTCGCGCGTGAGATCGGAAAGCAGCGCCATAACGGCGGCGGCAATTGCTCCAGAGCCTTGTAGCGCCCGGCCCAGAATAATTCCCCAGATGGAGTCGGAAAGCGCAGCGATAACGCTACcgg                                                                                                            <  1:2427105/150‑1 (MQ=255)
                                          aaCGCCATCGCTTTGGTGCGGTTTTGTTCGCGCGTGAGATCGGAAAGCAGCGCCATAACGGCGGCGGCAATTGCTCCAGAGCCTTGTAGCGCCCGGCCCAGAATCATTCCCCAGATGGAGTCGGAAAGCGCAGCGATAACGCTACcggcg                                                                                                          >  2:2941909/1‑150 (MQ=255)
                                           aCGCCATCGCTTTGGTGCGGTTTTGTTCGCGCGTGAGATCGGAAAGCAGCGCAATAACGGCGGCGGCAATTGCTCCAGAGCCTTGTAGCGCCCGGCCCTGAATCATTCCCCAGATGGAGTCGGAAAGCGCAGCGATAACGCTACcggcgg                                                                                                         <  1:3188090/150‑1 (MQ=255)
                                            cGCCATCGCTTTGGTGCGGTTTTGTTCGCGCGTGAGATCGGAAAGCAGCGCCATAACGGCGGCGGCAATTGCTCCAGAGCCTTGTAGCGCCCGGCCCAGAATAATTCCCCAGATGGAGTCGGAAAGCGCAGCGATAACGCTACCggcggc                                                                                                        >  1:2174193/1‑150 (MQ=255)
                                              ccATCGCTTTGGTGCGGTTTTGTTCGCGCGTGAGATCGGAAAGCAGCGCCATAACGGCGGCGGCAATTGCTCCAGAGCCTTGTAGCGCCCGGCCCAGAATAATTCCCCAGATGGAGTCGGAAAGCGCAGCGATAACGCTACCGGCGGCaa                                                                                                      >  2:4371549/1‑150 (MQ=255)
                                              ccATCGCTTTGGTGCGGTTTTGTTCGCGCGTGAGATCGGAAAGCAGCGCCATAACGGCGGCGGCAATTGCTCCAGAGCCTTGTAGCGCCCGGCCCAGAATAATTCCCCAGATGGAGTCGGAAAGCGCAGCGATAACGCTACCGGCGGCaa                                                                                                      >  2:1877985/1‑150 (MQ=255)
                                              ccATCGCTTTGGTGCGGTTTTGTTCGCGCGTGAGATCGGAAAGCAGCGCCATAACGGCGGCGGCAATTGCTCCAGAGCCTTGTAGCGCCCGGCCCAGAATAATTCCCCAGATGGAGTCGGAAAGCGCAGCGATAACGCTACCGGCGGCaa                                                                                                      <  1:4510423/150‑1 (MQ=255)
                                               cATCGCTTTGGTGCGGTTTTGTTCGCGCGTGAGATCGGAAAGCAGCGCCATAACGGCGGCGGCAATTTCTCCAGAGCCTTGTAGCGCCCGGCCCAGAATAATTCCCCAGATGGAGTCGGAAAGCGCAGCGATAACGCTACCGGCGGCaaa                                                                                                     >  1:405292/1‑150 (MQ=255)
                                               cATCGCTTTGGTGCGGTTTTGTTCGCGCGTGAGATCGGAAAGCAGCGCCATAACGGCGGCGGCAATTGCTCCAGAGCCTTGTAGCGCCCGGCCCAGAATAATTCCCCAGATGGAGTCGGAAAGCGCAGCGATAACGCTACCGGCGGCaaa                                                                                                     >  1:1899920/1‑150 (MQ=255)
                                               cATCGCTTTGGTGCGGTTTTGTTCGCGCGTGAGATCGGAAAGCAGCGCCATAACGGCGGCGGCAATTGCTCCAGAGCCTTGTAGCGCCCGGCCCAGAATAATTCCCCAGATGGAGTCGGAAAGCGCAGCGATAACGCTACCGGCGGCaaa                                                                                                     >  1:405186/1‑150 (MQ=255)
                                                  cGCTTTGGTGCGGTTTTGTTCGCGCGTGAGATCGGAAAGCAGCGCCATAACGGCGGCGGCAATTGCTCCAGAGCCTTGTAGCGCCCGGCCCAGAATAATTCCCCAGATGGAGTCGGAAAGCGCAGCGATAACGCTACCGGCGGCAAacac                                                                                                  <  2:175231/150‑1 (MQ=255)
                                                   gCTTTGGTGCGGTTTTGTTCGCGCGTGAGATCGGAAAGCAGCGCCATAACGGCGGCGGCAATTGCTCCAGAGCCTTGTAGCGCCCGGCCCAGAATCATTCCCCAGATGGAGTCGGAAAGCGCAGCGATAACGCTACCGGCGGCAAACAcc                                                                                                 >  1:141273/1‑150 (MQ=255)
                                                   gCTTTGGTGCGGTTTTGTTCGCGCGTGAGATCGGAAAGCAGCGCCATAACGGCGGCGGCAATTGCTCCAGAGCCTTGTAGCGCCCGGCCCAGAATCATTCCCCAGATGGAGTCGGAAAGCGCAGCGATAACGCTACCGGCGGCAAACAcc                                                                                                 >  1:141204/1‑150 (MQ=255)
                                                   gCTTTGGTGCGGTTTTGTTCGCGCGTGAGATCGGAAAGCAGCGCCATAACGGCGGCGGCAATTGCTCCAGAGCCTTGTAGCGCCCGGCCCAGAATAATTCCCCAGATGGAGTCGGAAAGCGCAGCGATAACGCTACCGGCGGCAAACAcc                                                                                                 >  2:1860077/1‑150 (MQ=255)
                                                       tGGTGCGGTTTTGTTCGCGCGTGAGATCGGAAAGCAGCGCCATAACGGCGGCGGCAATTGCTCCAGAGCCTTGTAGCGCCCGGCCCAGAATAATTCCCCAGATGGAGTCGGAAAGCGCAGCGATAACGCTACCGGCGGCAAACACCGCCa                                                                                             <  1:755616/150‑1 (MQ=255)
                                                       tGGTGCGGTTTTGTTCGCGCGTGAGATCGGAAAGCAGCGCCATAACGGCGGCGGCAATTGCTCCAGAGCCTTGTAGCGCCCGGCCCAGAATAATTCCCCAGATGGAGTCGGAAAGCGCAGCGATAACGCTACCGGCGGCAAACACCGCCa                                                                                             <  2:3059681/150‑1 (MQ=255)
                                                          tGCGGTTTTGTTCGCGCGTGAGATCGGAAAGCAGCGCCATAACGGCGGCGGCAATTGCTCCAGAGCCTTGTAGCGCCCGGCCCAGAATAATTCCCCAGATGGAGTCGGAAAGCGCAGCGATAACGCTACCGGCGGCAAACACCGCCAGcc                                                                                          >  2:2862958/1‑150 (MQ=255)
                                                           gCGGTTTTGTTCGCGCGTGAGATCGGAAAGCAGCGCCATAACGGCGGCGGCAATTGCTCCAGAGCCTTGTAGCGCCCGGCCCAGAATAATTCCCCAGATGGAGTCGGAAAGCGCAGCGATAACGCTACCGGCGGCAAACACCGCCAGccc                                                                                         >  1:2810982/1‑150 (MQ=255)
                                                           gCGGTTTTGTTCGCGCGTGAGATCGGAAAGCAGCGCCATAACGGCGGCGGCAATTGCTCCAGAGCCTTGTAGCGCCCGGCCCAGAATAATTCCCCAGATGGAGTCGGAAAGCGCAGCGATAACGCTACCGGCGGCAAACACCGCCAGccc                                                                                         <  2:2427034/150‑1 (MQ=255)
                                                              gTTTTGTTCGCGCGTGAGATCGGAAAGCAGCGCCATAACGGCGGCGGCAATTGCTCCAGAGCCTTGTAGCGCCCGGCCCAGAATCATTCCCCAGATGGAGTCGGAAAGCGCAGCGATAACGCTACCGGCGGCAAACACCGCCAGCCCAcc                                                                                      >  2:1716988/1‑150 (MQ=255)
                                                                 ttGTTCGCGCGTGAGATCGGAAAGCAGCGCCATAACGGCGGCGGCAATTGCTCCAGAGCCTTGTAGCGCCCGGCCCAGAATCATTCCCCAGATGGAGTCGGAAAGCGCAGCGATAACGCTACCGGCGGCAAACACCGCCAGCCCACCGAc                                                                                   <  2:325553/150‑1 (MQ=255)
                                                                    ttCGCGCGTGAGATCGGAAAGCAGCGCCATAACGGCGGCGGCAATTGCTCCAGAGCCTTGTAGCGCCCGGCCCAGAATAATTCCCCAGATGGAGTCGGAAAGCGCAGCGATAACGCTACCGGCGGCAAACACCGCCAGCCCACCGACAAt                                                                                <  1:1957044/150‑1 (MQ=255)
                                                                     tCGCGCGTGAGATCGGAAAGCAGCGCCATAACGGCGGCGGCAATTGCTCCAGAGCCTTGTAGCGCCCGGCCCAGAATAATTCCCCAGATGGAGTCGGAAAGCGCAGCGATAACGCTACCGGCGGCAAACACCGCCAGCCCACCGACAAtt                                                                               <  1:1223547/150‑1 (MQ=255)
                                                                     tCGCGCGTGAGATCGGAAAGCAGCGCCATAACGGCGGCGGCAATTGCTCCAGAGCCTTGTAGCGCCCGGCCCAGAATAATTCCCCAGATGGAGTCGGAAAGCGCAGCGATAACGCTACCGGCGGCAAACACCGCCAGCCCACCGACAAtt                                                                               <  1:1224133/150‑1 (MQ=255)
                                                                      cgcgcgTGAGATCGGAAAGCAGCGCCATAACGGCGGCGGCAATTGCTCCAGAGCCTTGTAGCGCCCGGCCCAGAATAATTCCCCAGATGGAGTCGGAAAGCGCAGCGATAACGCTACCGGCGGCAAACACCGCCAGCCCACCGACAATTa                                                                              <  1:3568974/150‑1 (MQ=255)
                                                                        cgcgTGAGATCGGAAAGCAGCGCCATAACGGCGGCGGCAATTGCTCCAGAGCCTTGTAGCGCCCGGCCCAGAATAATTCCCCAGATGGAGTCGGAAAGCGCAGCGATAACGCTACCGGCGGCAAACACCGCCAGCCCACCGACAATTAAt                                                                            >  1:816308/1‑150 (MQ=255)
                                                                          cgTGAGATCGGAAAGCAGCGCCATAACGGCGGCGGCAATTGCTCCAGAGCCTTGTAGCGCCCGGCCCAGAATCATTCCCCAGATGGAGTCGGAAAGCGCAGCGATAACGCTACCGGCGGCAAACACCGCCAGCCCACCGACAATTAATgg                                                                          <  1:3990391/150‑1 (MQ=255)
                                                                          cgTGAGATCGGAAAGCAGCGCCATAACGGCGGCGGCAATTGCTCCAGAGCCTTGTAGCGCCCGGCCCAGAATAATTCCCCAGATGGAGTCGGAAAGCGCAGCGATAACGCTACCGGCGGCAAACACCGCCAGCCCACCGACAATTAATgg                                                                          <  2:1067154/150‑1 (MQ=255)
                                                                             gagaTCGGAAAGCAGCGCCATAACGGCGGCGGCAATTGCTCCAGAGCCTTGTAGCGCCCGGCCCAGAATAATTCCCCAGATGGAGTCGGAAAGCGCAGCGATAACGCTACCGGCGGCAAACACCGCCAGCCCACCGACAATTAATGGttt                                                                       <  1:4104108/150‑1 (MQ=255)
                                                                              agaTCGGAAAGCAGCGCCATAACGGCGGCGGCAATTGCTCCAGAGCCTTGTAGCGCCCGGCCCAGAATAATTCCCCAGATGGAGTCGGAAAGCGCAGCGATAACGCTACCGGCGGCAAACACCGCCAGCCCACCGACAATTAATGGTTTg                                                                      >  2:1565401/1‑150 (MQ=255)
                                                                                 tCGGAAAGCAGCGCCATAACGGCGGCGGCAATTGCTCCAGAGCCTTGTAGCGCCCGGCCCAGAATAATTCCCCAGATGGAGTCGGAAAGCGCAGCGATAACGCTACCGGCGGCAAACACCGCCAGCCCACCGACAATTAATGGTTTGCGa                                                                   >  1:2204527/1‑150 (MQ=255)
                                                                                  cGGAAAGCAGCGCCATAACGGCGGCGGCAATTGCTCCAGAGCCTTGTAGCGCCCGGCCCAGAATCATTCCCCAGATGGAGTCGGAAAGCGCAGCGATAACGCTACCGGCGGCAAACACCGCCAGCCCACCGACAATTAATGGTTTGCGAc                                                                  >  2:2789195/1‑150 (MQ=255)
                                                                                   ggAAAGCAGCGCCATAACGGCGGCGGCAATTGCTCCAGAGCCTTGTAGCGCCCGGCCCAGAATCATTCCCCAGATGGAGTCGGAAAGCGCAGCGATAACGCTACCGGCGGCAAACACCGCCAGCCCACCGACAATTAATGGTTTGCGAcc                                                                 >  2:1942581/1‑150 (MQ=255)
                                                                                   ggAAAGCAGCGCCATAACGGCGGCGGCAATTGCTCCAGAGCCTTGTAGCGCCCGGCCCAGAATCATTCACCAGATGGAGTCGGAAAGCGCAGCGATAACGCTACCGGCGGCAAACACCGCCAGCCCACCGACAATTAATGGTTTGCGAcc                                                                 <  1:657646/150‑1 (MQ=255)
                                                                                    gAAAGCAGCGCCATAACGGCGGCGGCAATTGCTCCAGAGCCTTGTAGCGCCCGGCCCAGAATAATTCCCCAGATGGAGTCGGAAAGCGCAGCGATAACGCTACCGGCGGCAAACACCGCCAGCCCACCGACAATTAATGGTTTGCGACCa                                                                >  2:4533304/1‑150 (MQ=255)
                                                                                      aaGCAGCGCCATAACGGCGGCGGCAATTGCTCCAGAGCCTTGTAGCGCCCGGCCCAGAATAATTCCCCAGATGGAGTCGGAAAGCGCAGCGATAACGCTACCGGCGGCAAACACCGCCAGCCCACCGACAATTAATGGTTTGCGACCAAt                                                              <  2:3335632/150‑1 (MQ=255)
                                                                                       agcagcGCCATAACGGCGGCGGCAATTGCTCCAGAGCCTTGTAGCGCCCGGCCCAGAATCATTCCCCAGATGGAGTCGGAAAGCGCAGCGATAACGCTACCGGCGGCAAACACCGCCAGCCCACCGACAATTAATGGTTTGCGACCAATg                                                             >  1:2585997/1‑150 (MQ=255)
                                                                                       agcagcGCCATAACGGCGGCGGCAATTGCTCCAGAGCCTTGTAGCGCCCGGCCCAGAATAATTCCCCAGATGGAGTCGGAAAGCGCAGCGATAACGCTACCGGCGGCAAACACCGCCAGCCCACCGACAATTAATGGTTTGCGACCAATg                                                             <  2:2816395/150‑1 (MQ=255)
                                                                                       agcagcGCCATAACGGCGGCGGCAATTGCTCCAGAGCCTTGTAGCGCCCGGCCCAGAATAATTCCCCAGATGGAGTCGGAAAGCGCAGCGATAACGCTACCGGCGGCAAACACCGCCAGCCCACCGACAATTAATGGTTTGCGACCAATg                                                             >  2:4453025/1‑150 (MQ=255)
                                                                                         cagcGCCATAACGGCGGCGGCAATTGCTCCAGAGCCTTGTAGCGCCCGGCCCAGAATAATTCCCCAGATGGAGTCGGAAAGCGCAGCGATAACGCTACCGGCGGCAAACACCGCCAGCCCACCGACAATTAATGGTTTGCGACCAATGCg                                                           <  2:3639974/150‑1 (MQ=255)
                                                                                         cagcGCCATAACGGCGGCGGCAATTGCTCCAGAGCCTTGTAGCGCCCGGCCCAGAATAATTCCCCAGATGGAGTCGGAAAGCGCAGCGATAACGCTACCGGCGGCAAACACCGCCAGCCCACCGACAATTAATGGTTTGCGACCAATGCg                                                           <  1:3252276/150‑1 (MQ=255)
                                                                                          agcGCCATAACGGCGGCGGCAATTGCTCCAGAGCCTTGTAGCGCCCGGCCCAGAATAATTCCCCAGATGGAGTCGGAAAGCGCAGCGATAACGCTACCGGCGGCAAACACCGCCAGCCCACCGACAATTAATGGTTTGCGACCAATGCgg                                                          >  1:3755995/1‑150 (MQ=255)
                                                                                           gcgcCATAACGGCGGCGGCAATTGCTCCAGAGCCTTGTAGCGCCCGGCCCAGAATAATTCCCCAGATGGAGTCGGAAAGCGCAGCGATAACGCTACCGGCGGCAAACACCGCCAGCCCACCGACAATTAATGGTTTGCGACCAATGCGGt                                                         <  2:1374312/150‑1 (MQ=255)
                                                                                           gcgcCATAACGGCGGCGGCAATGGCTCCAGAGCCTTGTAGCGCCCGGCCCAGAATACTTCCCCAGATGGAGTCGGAAAGCGCAGCGATAACGCTACCGGCGGCAAACCCCGCCAGCCCACCGACAATTAATGGTTTGCGACCAATGCGGt                                                         <  2:3657330/150‑1 (MQ=255)
                                                                                                aTAACGGCGGCGGCAATTGCTCCAGAGCCTTGTAGCGCCCGGCCCAGAATAATTCCCCAGATGGAGTCGGAAAGCGCAGCGATAACGCTACCGGCGGCAAACACCGCCAGCCCACCGACAATTAATGGTTTGCGACCAATGCGGTCGGaa                                                    >  1:1368580/1‑150 (MQ=255)
                                                                                                 tAACGGCGTCGGCAATTGATCCAGAGCCTTGTAGCGCCCGGCCCAGAATAATACCCCAGATGGAGTCGGAAAGCGCAGCGATAACGCTCCCGGCGGCAAACACCGCCAGCCCACCGACAATTAATTGATTGCGACCAATGCGGTCGGaaa                                                   >  2:3423116/1‑150 (MQ=255)
                                                                                                 tAACGGCGGCGGCAATTGCTCCAGAGCCTTGTAGCGCCCGGCCCAGAATAATTCCCCAGATGGAGTCGGAAAGCGCAGCGATAACGCTACCGGCGGCAAACACCGCCAGCCCACCGACAATTAATGGTTTGCGACCAATGCGGTCGGaaa                                                   >  2:1137062/1‑150 (MQ=255)
                                                                                                  aaCGGCGGCGGCAATTGCTCCAGAGCCTTGTAGCGCCCGGCCCAGAATAATTCCCCAGATGGAGTCGGAAAGCGCAGCGATAACGCTACCGGCGGCAAACACCGCCAGCCCACCGACAATTAATGGTTTGCGACCAATGCGGTCGGAAAg                                                  >  1:2128486/1‑150 (MQ=255)
                                                                                                    cggcggcggCAATTGCTCCAGAGCCTTGTAGCGCCCGGCCCAGAATAATTCCCCAGATGGAGTCGGAAAGCGCAGCGATAACGCTACCGGCGGCAAACACCGCCAGCCCACCGACAATTAATGGTTTGCGACCAATGCGGTCGGAAAGCa                                                <  2:4018831/150‑1 (MQ=255)
                                                                                                      gcggcggcAATTGCTCCAGAGCCTTGTAGCGCCCGGCCCAGAATAATTCCCCAGATGGAGTCGGAAAGCGCAGCGATAACGCTACCGGCGGCAAACACCGCCAGCCCACCGACAATTAATGGTTTGCGACCAATGCGGTCGGAAAGCAgg                                              <  2:2603994/150‑1 (MQ=255)
                                                                                                        ggcggcAATTGCTCCAGAGCCTTGTAGCGCCCGGCCCAGAATAATTCCCCAGATGGAGTCGGAAAGCGCAGCGATAACGCTACCGGCGGCAAACACCGCCAGCCCACCGACAATTAATGGTTTGCGACCAATGCGGTCGGAAAGCAGGcc                                            <  1:4258059/150‑1 (MQ=255)
                                                                                                        ggcggcAATTGCTCCAGAGCCTTGTAGCGCCCGGCCCAGAATAATTCCCCAGATGGAGTCGGAAAGCGCAGCGATAACGCTACCGGCGGCAAACACCGCCAGCCCACCGACAATTAATGGTTTGCGACCAATGCGGTCGGAAAGCAGGcc                                            >  1:2576580/1‑150 (MQ=255)
                                                                                                           ggcAATTGCTCCAGAGCCTTGTAGCGCCCGGCCCAGAATAATTCCCCAGATGGAGTCGGAAAGCGCAGCGATAACGCTACCGGCGGCAAACACCGCCAGCCCACCGACAATTAATGGTTTGCGACCAATGCGGTCGGAAAGCAGGCCaaa                                         >  1:2062720/1‑150 (MQ=255)
                                                                                                           ggcAATTGCTCCAGAGCCTTGTAGCGCCCGGCCCAGAATAATTCCCCAGATGGAGTCGGAAAGCGCAGCGATAACGCTACCGGCGGCAAACACCGCCAGCCCACCGACAATTAATGGTTTGCGACCAATGCGGTCGGAAAGCAGGCCaaa                                         >  1:1084382/1‑150 (MQ=255)
                                                                                                            gcAATTGCTCCAGAGCCTTGTAGCGCCCGGCCCAGAATAATTCCCCAGATGGAGTCGGAAAGCGCAGCGATAACGCTACCGGCGGCAAACACCGCCAGCCCACCGACAATTAATGGTTTGCGACCAATGCGGTCGGAAAGCAGGCCAAAc                                        <  1:2986196/150‑1 (MQ=255)
                                                                                                                 tGCTCCAGAGCCTTGTAGCGCCCGGCCCAGAATAATTCCCCAGATGGAGTCGGAAAGCGCAGCGATAACGCTACCGGCGGCAAACACCGCCAGCCCACCGACAATTAATGGTTTGCGACCAATGCGGTCGGAAAGCAGGCCAAACGGAAt                                   <  2:3904551/150‑1 (MQ=255)
                                                                                                                   cTCCAGAGCCTTGTAGCGCCCGGCCCAGAATAATTCCCCAGATGGAGTCGGAAAGCGCAGCGATAACGCTACCGGCGGCAAACACCGCCAGCCCACCGACAATTAATGGTTTGCGACCAATGCGGTCGGAAAGCAGGCCAAACGGActct                                 >  2:845274/1‑150 (MQ=255)
                                                                                                                    tCCAGAGCCTTGTAGCGCCCGGCCCAGAATAATTCCCCAGATGGAGTCGGAAAGCGCAGCGATAACGCTACCGGCGGCAAACACCGCCAGCCCACCGACAATTAATGGTTTGCGACCAATGCGGTCGGAAAGCAGGCCAAACGGAATCTg                                >  2:1688854/1‑150 (MQ=255)
                                                                                                                       agagCCTTGTAGCGCCCGGCCCAGAATAATTCCCCAGATGGAGTCGGAAAGCGCAGCGATAACGCTACCGGCGGCAAACACCGCCAGCCCACCGACAATTAATGGTTTGCGACCAATGCGGTCGGAAAGCAGGCCAAACGGAATCTGaaa                             <  1:3205688/150‑1 (MQ=255)
                                                                                                                           ccTTGTAGCGCCCGGCCCAGAATAATTCCCCAGATGGAGTCGGAAAGCGCAGCGATAACGCTACCGGCGGCAAACACCGCCAGCCCACCGACAATTAATGGTTTGCGACCAATGCGGTCGGAAAGCAGGCCAAACGGAATCTGAAAAACg                         >  1:65093/1‑150 (MQ=255)
                                                                                                                            cTTGTAGCGCCCGGCCCAGACTCATTCCCCAGATGGAGTCGGAAAGCGCAGCGATAACGCTACCGGCGGCAAACACCGCCAGCCCACCGACAATTAATGGTTTGCGACCAATGCGGTCGGAAAGCAGGCCAAACGGAATCTGAAAAACgg                        <  1:346088/150‑1 (MQ=255)
                                                                                                                            cTTGTAGCGCCCGGCCCAGAATCATTCCCCAGATGGAGTCGGAAAGCGCAGCGATAACGCTACCGGCGGCAAACACCGCCAGCCCACCGACAATTAATGGTTTGCGACCAATGCGGTCGGAAAGCAGGCCAAACGGAATCTGAAAAACgg                        >  1:2077924/1‑150 (MQ=255)
                                                                                                                                     cccGGCCCAGAATAATTCCCCAGATGGAGTCGGAAAGCGCAGCGATAACGCTACCGGCGGCAAACACCGCCAGCCCACCGACAATTAATGGTTTGCGACCAATGCGGTCGGAAAGCAGGCCAAACGGAATCTGAAAAACGGCCTGAGTCa               >  2:2560662/1‑150 (MQ=255)
                                                                                                                                      ccGGCCCAGAATAATTCCCCAGATGGAGTCGGAAAGCGCAGCGATAACGCTACCGGCGGCAAACACCGCCAGCCCACCGACAATTAATGGTTTGCGACCAATGCGGTCGGAAAGCAGGCAAAACGGAATCTGAAAAACGGCCTGAGTCAg              <  1:4437881/150‑1 (MQ=255)
                                                                                                                                       cGGCCCAGAATAATTCCCCAGATGGAGTCGGAAAGCGCAGCGATAACGCTACCGGCGGCAAACACCGCCAGCCCACCGACAATTAATGGTTTGCGACCAATGCGGTCGGAAAGCAGGCCAAACGGAATCTGAAAAACGGCCTGAGTCAGa             >  1:4394883/1‑150 (MQ=255)
                                                                                                                                        ggCCCAGAATCATTCCCCAGAGGGAGTCGGAAAGCGCAGCGATAACGCTACCGGCGGCAAACACCGCCAGCCCACCGACAATTAATGGTTTGCGACCAATGCGGTCGGAAAGCAGGCCCAACGGAATCTGAAAAACGGCCTGAGTCAGAc            >  2:1450992/1‑150 (MQ=255)
                                                                                                                                        ggCCCAGAATAATTCCCCAGATGGAGTCGGAAAGCGCAGCGATAACGCTACCGGCGGCAAACACCGCCAGCCCACCGACAATTAATGGTTTGCGACCAATGCGGTCGGAAAGCAGGCCAAACGGAATCTGAAAAACGGCCTGAGTCAGAc            >  1:462022/1‑150 (MQ=255)
                                                                                                                                         gCCCAGAATAATTCCCCAGATGGAGTCGGAAAGCGCAGCGATAACGCTACCGGCGGCAAACACCGCCAGCCCACCGACAATTAATGGTTTGCGACCAATGCGGTCGGAAAGCAGGCCAAACGGAATCTGAAAAACGGCCTGAGTCAGAcc           <  2:4314428/150‑1 (MQ=255)
                                                                                                                                            cAGAATAATTCCCCAGATGGAGTCGGAAAGCGCAGCGATAACGCTACCGGCGGCAAACACCGCCAGCCCACCGACAATTAATGGTTTGCGACCAATGCGGTCGGAAAGCAGGCCAAACGGAATCTGAAAAACGGCCTGAGTCAGACCATa        >  1:3837392/1‑150 (MQ=255)
                                                                                                                                            cAGAATAATTCCCCAGATGGAGTCGGAAAGCGCAGCGATAACGCTACCGGCGGCAAACACCGCCAGCCCACCGACAATTAATGGTTTGCGACCAATGCGGTCGGAAAGCAGGCCAAACGGAATCTGAAAAACGGCCTGAGTCAGACCATa        >  2:1903144/1‑150 (MQ=255)
                                                                                                                                            aaGAATAATTCCCCAGTTGGAGGCGGAAAGCGCAGCTATAACGCTACCGGCGGAAAACACCGCCAGCCCACCGACAATTATTGGTTTGCGACCAATGCGGTCGGAAAGCAGGCCAAACGGAATCTGAAAAACGGCCTGAGTCAGACCATa        <  1:3465473/149‑1 (MQ=255)
                                                                                                                                             aGAATAATTCCCCAGATGGAGTCGGAAAGCGCAGCGATAACGCTACCGGCGGCAAACACCGCCAGCCCACCGACAATTAATGGTTTGCGACCAATGCGGTCGGAAAGCAGGCCAAACGGAATCTGAAAAACGGCCTGAGTCAGACCATaa       >  1:1506450/1‑150 (MQ=255)
                                                                                                                                                 taatTCCCCAGATGGAGTCGGAAAGCGCAGCGATAACGCTACCGGCGGCAAACACCGCCAGCCCACCGACAATTAATGGTTTGCGACCAATGCGGTCGGAAAGCAGGCCAACCGGAATCTGAAAAACGGCCTGAGTCAGACCATAAATAc   <  2:3024719/150‑1 (MQ=255)
                                                                                                                                                  aatTCCCCAGATGGAGTCGGAAAGCGCAGCGATAACGCTACCGGCGGCAAACACCGCCAGCCCACCGACAATTAATGGTTTGCGACCAATGCGGTCGGAAAGCAGGCCAAACGGAATCTGAAAAACGGCCTGAGTCAGACCATAAATAcc  >  1:1114946/1‑150 (MQ=255)
                                                                                                                                                  |                                                                                                                                                     
ACCATCGCAATGGCAAAGGTAATGCCAAAGCTCACGCCGATAAACGCCATCGCTTTGGTGCGGTTTTGTTCGCGCGTGAGATCGGAAAGCAGCGCCATAACGGCGGCGGCAATTGCTCCAGAGCCTTGTAGCGCCCGGCCCAGAATAATTCCCCAGATGGAGTCGGAAAGCGCAGCGATAACGCTACCGGCGGCAAACACCGCCAGCCCACCGACAATTAATGGTTTGCGACCAATGCGGTCGGAAAGCAGGCCAAACGGAATCTGAAAAACGGCCTGAGTCAGACCATAAATACC  >  CP009273/441665‑441960

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑