Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 27,603 T→G 10.2% F104C (TTT→TGT)  rihC → ribonucleoside hydrolase 3

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP00927327,6030TG10.2% 903.5 / 26.8 314F104C (TTT→TGT) rihCribonucleoside hydrolase 3
Reads supporting (aligned to +/- strand):  ref base T (86/188);  new base G (37/3);  total (123/191)
Fisher's exact test for biased strand distribution p-value = 6.26e-14
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 329 total aligned reads displayed.

TTCTGGAATGCGGAGATTCCGCTCGCCCAAGGGGCCGCTGTGCCACTGGTACGCGCACCGCGTGATGCGGCATCTGTGCACGGCGAATCGGGAATGGCTGGCTACGACTTTGTTGAGCACAACCGAAAGCCGCTCGGGATACCGGCGTTTCTGGCGATTCGGGATGCCCTGATGCGTGCACCAGAGCCTGTTACCCTGGTGGCCATCGGCCCGTTAACCAATATTGCGCTGTTACTTTCACAATGCCCGGAATGCAAGCCGTATATTCGCCGTCTGGTGATCATGGGTGGTTCTGCC  >  CP009273/27455‑27751
                                                                                                                                                    |                                                                                                                                                    
ttCTGGAATGCGGAGATTCCGCTCGCCCAAGGGGCCGCTGTGCCACTGGTACGCGCACCGCGTGATGCGGCATCTGTGCACGGCGAATCGGGAATGGCTGGCTACGACTTTGTTGAGCACAACCGAAAGCCGCTCGGGATACCGGCGtt                                                                                                                                                      <  2:4789638/149‑1 (MQ=255)
ttCTGGAATGCGGAGATTCCGCTCGCCCAAGGGGCCGCTGTGCCACTGGTACGCGCACCGCGTGATGCGGCATCTGTGCACGGCGAATCGGGAATGGCTGGCTACGACTTTGTTGAGCACAACCGAAAGCCGCTCGGGATACCGGCGtt                                                                                                                                                      <  2:2069358/149‑1 (MQ=255)
 tCTGGAATGCGGAGATTCCGCTCGCCCAAGGGGCCGCTGTGCCACTGGTACGCGCACCGCGTGATGCGGCATCTGTGCACGGCGAATCGGGAATGGCTGGCTACGACTTTGTTGAGCACAACCGAAAGCCGCTCGGGATACCGGCGttt                                                                                                                                                     >  2:1396093/1‑149 (MQ=255)
   tGGAATGCGGAGATTCCGCTCGCCCAAGGGGCCGCTGTGCCACTGGTACGCGCACCGCGTGATGCGGCATCTGTGCACGGCGAATCGGGAATGGCTGGCTACGACTTTGTTGAGCACAACCGAAAGCCGCTCGGGATACCGGCGTTTCt                                                                                                                                                   >  2:5075986/1‑149 (MQ=255)
       aTGCGGAGATTCCGCTCGCCCAAGGGGCCGCTGTGCCACTGGTACGCGCACCGCGTGATGCGGCATCTGTGCACGGCGAATCGGGAATGGCTGGCTACGACTTTGTTGAGCACAACCGAAAGCCGCTCGGGATACCGGCGTGTCTGGCg                                                                                                                                               >  1:1224845/1‑149 (MQ=255)
        tacGGAGATTCCGCTCGCCCAAGGGGCTGCTGTGCCACTGGTACGCGCACCGCGTGATGCGGCATCTGTGCACGGCGAATCGGGAATGGCTGGCTACGACTTTGTTGAGCACAACCGAAAGCCGCTCGGGATACCGGCGTTTCTGGCGa                                                                                                                                              <  2:4421449/147‑1 (MQ=255)
         gCGGAGATTCCGCTCGCCCAAGGGGCCGCTGTGCCACTGGTACGCGCACCGCGTGATGCGGCATCTGTGCACGGCGAATCGGGAATGGCTGGCTACGACTTTGTTGAGCACAACCGAAAGCCGCTCGGGATACCGGCGTTTCTGGCGAt                                                                                                                                             <  2:1841065/149‑1 (MQ=255)
          cGGAGATTCCGCTCGCCCAAGGGGCCGCTGTGCCACTGGTACGCGCACCGCGTGATGTGGCATCTGTGCACGGCGAATCGGGAATGGCTGGCTACGACTTTGTTGAGCACAACCGAAAGCCGCTCGGGATACCGGCGTTTCTGGCGAtt                                                                                                                                            <  2:4176363/149‑1 (MQ=255)
           ggAGATTCCGCTCGCCCAAGGGGCCGCTGTGCCACTGGTACGCGCACCGCGTGATGCGGCATCTGTGCACGGCGAATCGGTATTGGCTGGCTACGACTTTGTTGAGCACAACCGAAAGCCGCTCGGGATACCGGCGTTTCTGGCGATTc                                                                                                                                           <  1:4395378/149‑1 (MQ=255)
           ggAGATTCCGCTCGCCCAAGGGGCCGCTGTGCCACTGGTACGCGCACCGCGTGATGCGGCATCTGTGCACGGCGAATCGGGAATGGCTGGCTACGACTTTGTTGAGCACAACCGAAAGCCGCTCGGGATACCGGCGTTTCTGGCGATTc                                                                                                                                           <  1:1450132/149‑1 (MQ=255)
           ggAGATTCCGCTCGCCCAAGGGGCCGCTGTGCCACTGGTACGCGCACCGCGTGATGCGGCATCTGTGCACGGCGAATCGGGAATGGCTGGCTACGACTTTGTTGAGCACAACCGAAAGCCGCTCGGGATACCGGCGTTTCTGGCGATTc                                                                                                                                           <  1:5131661/149‑1 (MQ=255)
               aTTCCGCTCGCCCAAGGGGCCGCTGTGCCACTGGTACGCGCACCGCGTGATGCGGCATCTGTGCACGGCGAATCGGGAATGGCTGGCTACGACTTTGTTGAGCACAACCGAAAGCCGCTCGGGATACCGGCGTTTATGGCGATTCgggt                                                                                                                                       >  1:4864654/1‑148 (MQ=255)
                ttCCGCTCGCCCAAGGGGCCGCTGTGCCACTGGTACGCGCACCGCGTGATGCGGCATCTGTGCACGGCGAATCGGGAATGGCTGGCTACGACTTTGTTGAGCACAACCGAAAGCCGCTCGGGATACCGGCGTTTCTGGCGATTCGGGAt                                                                                                                                      <  1:740329/149‑1 (MQ=255)
                ttCCGCTCGCCCAAGGGGCCGCTGTGCCACTGGTACGCGCACCGCGTGATGCGGCATCTGTGCACGGCGAATCGGGAATGGCTGGCTACGACTTTGTTGAGCACAACCGAAAGCCGCTCGGGATACCGGCGTTTCTGGCGATTCGGGAt                                                                                                                                      <  2:1146065/149‑1 (MQ=255)
                ttCCGCTCGCCCAAGGGGCCGCTGTGCCACTGGTACGCGCACCGCGTGATGCGGCATCTGTGCACGGCGAATCGGGAATGGCTGGCTACGACTTTGTTGAGCACAACCGAAAGCCGCTCGGGATACCGGCGTTTCTGGCGATTCGGGAt                                                                                                                                      <  2:1732410/149‑1 (MQ=255)
                  ccGCTCGCCCAAGGGGCCGCTGTGCCACTGGTACGCGCACCGCGTGATGCGGCATCTGTGCACGGCGAATCGGGAATGGCTGGCTACGACTTTGTTGAGCACAACCGAAAGCCGCTCGGGATACCGGCGTTTCTGGCGATTCGGGATGc                                                                                                                                    <  2:1121131/149‑1 (MQ=255)
                    gCTCGCCCAAGGGGCCGCTGTGCCACTGGTACGCGCACCGCGTGATGCGGCATCTGTGCACGGCGAATCGGGAATGGCTGGCTCCGACTTTGTTGCGCACAACCGAAAGCCGCTCGGGATACCGGCGTTTCTGGCGATTCGGGATGccc                                                                                                                                  <  1:1426298/149‑1 (MQ=255)
                    gCTCGCCCAAGGGGCCGCTGTGCCACTGGTACGCGCACCGCGTGATGCGGCATCTGTGCACGGCGAATCGGGAATGGCTGGCTACGACTTTGTTGAGCACAACCGAAAGCCGCTCGGGATACCGGCGTTTCTGGCGATTCGGGATGccc                                                                                                                                  >  1:4781368/1‑149 (MQ=255)
                     cTCGCCCAAGGGGCCGCTGTGCCACTGGTACGCGCACCGCGTGATGCGGCATCTGTGCACGGCGAATCGGGAATGGCTGGCTACGACTTTGTTGAGCACAACCGAAAGCCGCTCGGGATACCGGCGTGTCTGGCGCTTCGGGCTGccca                                                                                                                                 >  1:4119424/1‑148 (MQ=255)
                        gCCCAAGGGGCCGCTGTGCCACTGTTACGCGCACCGCGTTATGCGGCATCTGTGCACGGCGAATCGGTAATGGCTGGCTACGACTTTGTTGAGCACAACCGAACGCCGCTCGGGATACCGGCGTTTCTGGCGATTCGGGATGCCCTGAt                                                                                                                              <  1:4069925/149‑1 (MQ=255)
                         cccAAGGGGCCGCTGTGCCACTGGTACGCGCACCGCGTGATGCGGCATCTGTGCACGGCGAATCGGGAATGGCTGGCTACGACTTTGTTGAGCACAACCGAAAGCCGCTCGGGATACCGGCGTTTCTGGCGATTCGGGATGCCCTGATg                                                                                                                             >  1:5037791/1‑149 (MQ=255)
                          ccAAGGGGCCGCTGTGCCACTGGTACGCGCACCGCGTGATGCGGCATCTGTGCACGGCGAATCGGGAATGGCTGGCTACGACTTTGTTGAGCACAACCGAAAGCCGCTCGGGATACCGGCGTTTCTGGCGATTCGGGATGCCCTGATGc                                                                                                                            >  1:2024563/1‑149 (MQ=255)
                          ccAAGGGGCCGCTGTGCCACTGGTACGCGCACCGCGTGATGCGGCATCTGTGCACGGCGAATCGGGAATGGCTGGCTACGACTTTGTTGAGCACAACCGAAAGCCGCTCGGGATACCGGCGTTTCTGGCGATTCGGGATGCCCTGATGc                                                                                                                            <  1:3303789/149‑1 (MQ=255)
                            aaGGGGCCGCTGTGCCACTGGTACGCGCACCGCGTGATGCGGCATCTGTGCACGGCGAATCGGGAATGGCTGGCTACGACTTTGTTGAGCACAACCGAAAGCCGCTCGGGATACCGGCGTTTCTGGCGATTCTGGATGCCCTGATGCGt                                                                                                                          <  2:3025412/149‑1 (MQ=255)
                                 gCCGCTGTGCCACTGGTACGCGCACCGCGTGATGCGGCATCTGTGCACGGCGAATCGGGAATGGCTGGCTACGACTTTGTTGAGCACAACCGAAAGCCGCTCGGGATACCGGCGTTTCTGGCGATTCGGGATGCCCTGATGCGTGCAcc                                                                                                                     <  1:2370546/149‑1 (MQ=255)
                                  ccGCTGTGCCACTGGTACGCGCACCGCGTGATGCGGCATCTGTGCACGGCGAATCGGGAATGGCTGGCTACGACTTTGTTGAGCACAACCGAAAGCCGCTCGGGATACCGGCGTGTCTGGCGATTCGGGATGCCCTGATGCGTGCACCa                                                                                                                    >  1:2511835/1‑149 (MQ=255)
                                        tgCCACTGGTACGCGCACCGCGTGATGCGGCATCTGTGCACGGCGAATCGGGAATGGCTGGCTACGACTTTGTTGAGCACAACCGAAAGCCGCTCGGGATACCGGCGTTTCTGGCGATTCGGGATGCCCTGATGCGTGCACCAGAGCCt                                                                                                              <  1:837804/149‑1 (MQ=255)
                                          ccACTGGTACGCGCACCGCGTGATGCGGCATCTGTGCACGGCGAATCGGGAATGGCTGGCTACGACTTTGTTGAGCACAACCGAAAGCCGCTCGGGATACCGGCGTTTCTGGCGATTCGGGATGCCCTGATGCGTGCACCAGAGCCTGt                                                                                                            <  2:2523798/149‑1 (MQ=255)
                                           cACTGGTACGCGCACCGCGTGATGCGGCATCTGTGCACGGCGAATCGGGAATGGCTGGCTACGACTTTGTTGAGCACAACCGAAAGCCGCTCGGGATACCGGCGTTTCTGGCGATTCGGGATGCCCTGATGCGTGCACCAGAGCCTGtt                                                                                                           <  1:4669050/149‑1 (MQ=255)
                                           cACTGGTACGCGCACCGCGTGATGCGGCATCTGTGCACGGCGAATCGGGAATGGCTGGCTACGACTTTGTTGAGCACAACCGAAAGCCGCTCGGGATACCGGCGTTTCTGGCGATTCGGGATGCCCTGATGCGTGCACCAGAGCCTGtt                                                                                                           <  1:1003111/149‑1 (MQ=255)
                                            aCTGGTACGCGCACCGCGTGATGCGGCATCTGTGCACGGCTAATCGGGAATGGCTGGCTACGACTTTGTTGAGCACAACCGAAAGCCGCTCGGGATACCGGCGTTTCTGGCGATTCGGGATGCCCTGATGCGTGCACCAGAGCCTGTTa                                                                                                          <  1:981079/149‑1 (MQ=255)
                                                 tACGCGCACCGCGTGATGCGGCATCTGTGCACGGCGAATCGGGAATGGCTGGCTACGACTTTGTTGAGCACAACCGAAAGCCGCTCGGGATACCGGCGTTTCTGGCGATTAGGGATGCCCAGATGCGTGCCCCACAGCCTGTTAaccag                                                                                                     >  1:3090217/1‑147 (MQ=255)
                                                  aCGCGCACCGCGTGATGCGGCATCTGTGCACGGCGAATCGGGAATGGCTGGCTACGACTTTGTTGAGCACAACCGAAAGCCGCTCGGGATACCGGCGTTTCTGGCGATTCGGGATGCCCTGATGCGTGCACCAGAGCCTGTTACCCtgg                                                                                                    >  2:2562135/1‑149 (MQ=255)
                                                   cgcgCACCGCGTGATGCGGCATCTGTGCACGGCGAATCGGGAATGGCTGGCTACGACTTTGTTGAGCACAACCGAAAGCCGCTCGGGATACCGGCGTTTCTGGCGATTCGGGATGCCCTGATGCGTGCACCAGAGCCTGTTACCCtggt                                                                                                   <  2:3319982/149‑1 (MQ=255)
                                                   cgcgCACCGCGTGATGCGGCATCTGTGCACGGCGAATCGGGAATGGCTGGCTACGACTTTGTTGAGCACAACCGAAAGCCGCTCGGGATACCGGCGTTTCTGGCGATTCGGGATGCCCTGATGCGTGCACCAGAGCCTGTTACCCtggt                                                                                                   <  2:3839018/149‑1 (MQ=255)
                                                   cgcgCACCGCGTGATGCGGCATCTGTGCACGGCGAATCGGGAATGGCTGGCTACGACTTTGTTGAGCACAACCGAAAGCCGCTCGGGATACCGGCGTTTCTGGCGATTCGGGATGCCCTGATGCGTGCACCAGAGCCTGTTACCCtggt                                                                                                   <  2:4007959/149‑1 (MQ=255)
                                                     cgcACCGCGTGATGCGGCATCTGTGCACGGCGAATCGGGAATGGCTGGCTACGACTTTGTTGAGCACAACCGAAAGCCGCTCGGGATACCGGCGTTTCTGGCGATTCGGGATGCCCTGATGCGTGCACCAGAGCCTGTTACCCtggtgg                                                                                                 <  1:1685293/149‑1 (MQ=255)
                                                     cgcACCGCGTGATGCGGCATCTGTGCACGGCGAATCGGGAATGGCTGGCTACGACTTTGTTGAGCACAACCGAAAGCCGCTCGGGATACCGGCGTTTCTGGCGATTCGGGATGCCCTGATGCGTGCACCAGAGCCTGTTACCCtggtgg                                                                                                 <  2:4140334/149‑1 (MQ=255)
                                                      gcACCGCGTGATGCGGCATCTGTGCACGGCGAATCGGGAATGGCTGGCTACGACTTTGTTGAGCACAACCGAAAGCCGCTCGGGATACCGGCGTTTCTGGCGATTCGGGATGCCCTGATGCGTGCACCAGAGCCTGTTACCCTGGTGGc                                                                                                <  1:1966186/149‑1 (MQ=255)
                                                       cACCGCGTGATGCGGCATCTGTGCACGGCGAATCGGGAATGGCTGGCTACGACTTTGTTGAGCACAACCGAAAGCCGCTCGGGATACCGGCGTTTCTGGCGATTCGGGATGCCCTGATGCGTGCACCAGAGCCTGTTACCCTGGTGGcc                                                                                               <  1:952076/149‑1 (MQ=255)
                                                       cACCGCGTGATGCGGCATCTGTGCACGGCGAATCGGGAATGGCTGGCTACGACTTTGTTGAGCACAACCGAAAGCCGCTCGGGATACCGGCGTTTCTGGCGATTCGGGATGCCCTGATGCGTGCACCAGAGCCTGTTACCCTGGTGGcc                                                                                               <  1:2553389/149‑1 (MQ=255)
                                                         ccGCGTGATGCGGCATCTGTGCACGGCGAATCGGGAATGGCTGGCTACGACTTTGTTGAGCACAACCGAAAGCCGCTCGGGATACCGGCGTTTCTGGCGATTCGGGATGCCCTGATGCGTGCACCAGAGCCTGTTACCCTGGTGGCCAt                                                                                             <  2:1276915/149‑1 (MQ=255)
                                                         ccGCGTGATGCGGCATCTGTGCACGGCGAATCGGGAATGGCTGGCTACGACTTTGTTGAGCACAACCGAAAGCCGCTCGGGATACCGGCGTTTCTGGCGATTCGGGATGCCCTGATGCGTGCACCAGAGCCTGTTACCCTGGTGGCCAt                                                                                             <  2:890527/149‑1 (MQ=255)
                                                          cgcgTGATGCGGCATCTGTGCACGGCGAATCGGGAATGGCTGGCTACGACTTTGTTGAGCACAACCGAAAGCCGCTCGGGATACCGGCGTTTCTGGCGATTCGGGATGCCCTGATGCGTGCACCAGAGCCTGTTACCCTGGTGGCCATc                                                                                            >  2:2338290/1‑149 (MQ=255)
                                                          cgcgTGATGCGGCATCTGTGCACGGCGAATCGGGAATGGCTGGCTACGACTTTGTTGAGCACAACCGAAAGCCGCTCGGGATACCGGCGTTTCTGGCGATTCGGGATGCCCTGATGCGTGCACCAGAGCCTGTTACCCTGGTGGCCATc                                                                                            <  2:1164472/149‑1 (MQ=255)
                                                          cgcgTGAGGCGGCATCTGTGCACGGCGAATCGGGAATGGCTGGCTACGACTTTGTTGAGCACAACCGAACGCCGCTCGGGATACCGGCGTTTCTGGCGATTCGGGATGCCCTGATGCGTGCACCAGAGCCTGTTACCCTGGTGGCCATc                                                                                            <  1:3915133/149‑1 (MQ=255)
                                                           gcgTGATGCGGCATCTGTGCACGGCGAATCGGGAATGGCTGGCTACGACTTTGTTGAGCACAACCGAAAGCCGCTCGGGATACCGGCGTTTGTGGCGATTCGGGATGCCCAGATGCGTGCACCAGAGCCTGTTAACCAGGTGGCCATCg                                                                                           >  1:1471685/1‑149 (MQ=255)
                                                             gTGATGCGGCATCTGTGCACGGCGAATCGGGAATGGCTGGCTACGACTTTGTTGAGCACAACCGAAAGCCGCTCGGGATACCGGCGTGTCTGGCGATTCGGGATGCCCAGATGCGTGCACCCGAGCCAGTTACCCAGGTGGCCATCGGc                                                                                         >  1:388460/1‑149 (MQ=255)
                                                                       aTCTGTGCACGGCGAATCGGGAATGGCTGGCTACGACTTTGTTGAGCACAACCGAAAGCCGCTCGGGATACCGGCGTGTCTGGCGATTAGGGATGCCCTGATGCGTGCACCAGAGCCAGTTAACCTGGTGGCCATCGGCCCGGTAACCa                                                                               >  1:2807132/1‑149 (MQ=255)
                                                                         cTGTGCACGGCGAATCGGGAATGGCTGGCTACGACTTTGTTGAGCACAACCGAAAGCCGCTCGGGATACCGGCGTTTCTGGCGATTCGGGATGCCCTGATGCGTGCACCAGAGCCTGTTACCCTGGTGGCCATCGGCCCGTTAACCAat                                                                             <  1:2296486/149‑1 (MQ=255)
                                                                         cTGTGCACGGCGAATCGGGAATGGCTGGCTACGACTTTGTTGAGCACAACCGAAAGCCGCTCGGGATACCGGCGTTTCTGGCGATTCGGGATGCCCTGATGCGTGCACCAGAGCCTGTTACCCTGGTGGCCATCGGCCCGTTAACCAat                                                                             <  1:3781328/149‑1 (MQ=255)
                                                                          tgtgCACGGCGAATCGGGAATGGCTGGCTACGACTTTGTTGAGCACAACCGAAAGCCGCTCGGGATACCGGCGTTTATTGCGATTGGGGATGGCCAGATGAGTGACACACAGCCTGTTAACCAGGTGGCCAACGGCCCGGTAACCaaaa                                                                            >  1:1333524/1‑147 (MQ=255)
                                                                            tgCACGGCGAATCGGGAATGGCTGGCTACGACTTTGTTGAGCACAACCGAAAGCCGCTCGGGATACCGGCGTGTGTGGCGATTAGGGGTGCCCAGATGCGTGCCCCCCAGCCCGTTACCCAGGTGGCCAACGGCCCCGTAAcccaaaat                                                                          >  1:1798656/1‑143 (MQ=255)
                                                                             gCACGGCGAATCGGGAATGGCTGGCTACGACTTTGTTGAGCACAACCGAAAGCCGCTCGGGATACCGGCGTTTCTGGCGATTAGGGATTCCCAGATTCGTGCACCAGAGCCTGTTAACCAGGTGGCCATCGGCCCGTTAACCAAAATTg                                                                         >  2:3511950/1‑149 (MQ=255)
                                                                                 ggCGAATCGGGATTGGCTGGCTACGACTTTGTTGAGCACAACCGAAAGCCGCTCGGGATACCGGCGTTTCTGGCGATTCGGGATGCCCTGATGCGTGCACCAGAGCCTGTTACCCTGGTGGCCATCGGCCCGTTAACCAATATTGCGCt                                                                     <  1:1397222/149‑1 (MQ=255)
                                                                                 ggCGAATCGGGAATGGCTGGCTACGACTTTGTTGAGCACAACCGAAAGCCGCTCGGGATACCGGCGTTTCTGGCGATTCGGGATGCCCTGATGCGTGCACCAGAGCCTGTTACCCTGGTGGCCATCGGCCCGTTAACCAATATTGCGCt                                                                     <  1:3631608/149‑1 (MQ=255)
                                                                                 ggCGAATCGGGAATGGCTGGCTACGACTTTGTTGAGCACAACCGAAAGCCGCTCGGGATACCGGCGTTTCTGGCGATTCGGGATGCCCTGATGCGTGCACCAGAGCCTGTTACCCTGGTGGCCATCGGCCCGTTAACCAATATTGCGCt                                                                     <  1:2895294/149‑1 (MQ=255)
                                                                                   cGAATCGGGAATGGCTGGCTACGACTTTGTTGAGCACAACCGAAAGCCGCTCGGGATACCGGCGTTTCTGGCGATTCGGGATGCCCTGATGCGTGCACCAGAGCCTGTTACCCTGGTGGCCATCGGCCCGTTAACCAATATTGCGCTGt                                                                   <  2:2242853/149‑1 (MQ=255)
                                                                                   cGAATCGGGAATGGCTGGCTACGACTTTGTTGAGCACAACCGAAAGCCGCTCGGGATACCGGCGTTTCTGGCGATTCGGGATGCCCTGATGCGTGCACCAGAGCCTGTTACCCTGGTGGCCATCGGCCCGTTAACCAATATTGCGCTGt                                                                   <  1:601708/149‑1 (MQ=255)
                                                                                    gAATCGGGAATGGCTGGCTACGACTTTGTTGAGCACAACCGAAAGCCGCTCGGGATACCGGCGTTTCTGGCGATTCGGGATGCCCTGATGCGTGCACCAGAGCCTGTTACCCTGGTGGCCATCGGCCCGTTAACCAATATTGCGCTGtt                                                                  <  2:631236/149‑1 (MQ=255)
                                                                                    gAATCGGGAATGGCTGGCTACGACTTTGTTGAGCACAACCGAAAGCCGCTCGGGATACCGGCGTTTCTGGCGATTCGGGATGCCCTGATGCGTGCACCAGAGCCTGTTACCCTGGTGGCCATCGGCCCGTTAACCAATATTGCGCTGtt                                                                  <  2:2184696/149‑1 (MQ=255)
                                                                                     aaTCGGGAATGGCTGGCTACGACTTTGTTGAGCACAACCGAAAGCCGCTCGGGATACCGGCGTTTCTGGCGATTAGGGATGCCCTGATGCGTGCACCAGAGCCTGTTACCCTGGTGGCCATCGGCCCCTTAACCAATATTGCGCTGTTa                                                                 >  2:3633553/1‑149 (MQ=255)
                                                                                     aaTCGGGAATGGCTGGCTACGACTTTGTTGAGCACAACCGAAAGCCGCTCGGGATACCGGCGTGTGTGGCGATTAGGGATGGCCAGATGAGTGCACCACAGCCTGTTAACCAGGTGGCCAACGGCCCCTTAAACAAAAATTAGCTGTTa                                                                 >  1:3075249/1‑149 (MQ=255)
                                                                                      aTCGGGAATGGCTGGCTACGACTTTGTTGAGCACAACCGAAAGCCGCTCGGGATACCGGCGTTTCTGGCGATTCGGGATGCCCTGATGCGTGCACCAGAGCCTGTTACCCTGGTGGCCATCGGCCCGTTAACCAATATTGCGCTGTTAc                                                                <  2:1436085/149‑1 (MQ=255)
                                                                                            aaTGGCTGGCTACGACTTTGTTGAGCACAACCGAAAGCCGCTCGGGATACCGGCGTTTCTGGCGATTCGGGATGCCCTGATGCGTGCACCAGAGCCTGTTACCCTGGTGGCCATCGGCCCGTTAACCAAAATTGCGCTGTTACTTTcac                                                          >  2:3125489/1‑149 (MQ=255)
                                                                                              tggcgggcTACGACTTTGTTGAGCACAACCGAAAGCCGCTCGGGATACCGGCGTTTCTGGCGATTCGGGATGCCCTGATGCGTGCACCAGAGCCTGTTACCCTGGTGGCCATCGGCCCGTTAACCAATATTGCGCTGTTACTTTCACaa                                                        <  2:3492155/149‑1 (MQ=255)
                                                                                               ggctggctACGACTTTGTTGAGCACAACCGAAAGCCGCTCGGGATACCGGCGTTTCTGGCGATTCGGGATGCCCTGATGCGTGCACCAGAGCCTGTTACCCTGGTGGCCATCGGCCCGTTAACCAATATTGCGCTGTTACTTTCACAAt                                                       <  2:1606179/149‑1 (MQ=255)
                                                                                                   ggctACGACTTTGTTGAGCACAACCGAAAGCCGCTCGGGATACCGGCGTTTCTGGCGATTCGGGATGCCCTGATGCGTGCACCAGAGCCTGTTACCCTGGTGGCCATCGGCCCGTTAACCAATATTGCGCTGTTACTTTCACAATGccc                                                   <  1:1486993/149‑1 (MQ=255)
                                                                                                   ggctACGACTTTGTTGAGCACAACCGAAAGCCGCTCGGGATACCGGCGTTTCTGGCGATTCGGGATGCCCTGATGCGTGCACCAGAGCCTGTTACCCTGGTGGCCATCGGCCCGTTAACCAATATTGCGCTGTTACTTTCACAATGccc                                                   <  2:5165087/149‑1 (MQ=255)
                                                                                                       aCGACTTTGTTGAGCACAACCGAAAGCCGCTCGGGATACCGGCGTTTCTGGCGATTCGGGATGCCCTGATGCGTGCACCAGAGCCTGTTACCCTGGTGGCCATCGGCCCGTTAACCAATATTGCGCTGTTACTTTCACAATGCCCGGaa                                               <  2:732833/149‑1 (MQ=255)
                                                                                                        cGACTTTGTTGAGCACAACCGAAAGCCGCTCGGGATACCGGCGTTTCTGGCGATTAGGGATGCCCTGATGCGTGCACCAGAGCCTGTTACCCAGGTGGCCATCGGCCCGTTAACCCATATTTCGCAGTTACTTTAACAATGCCCGGAAt                                              >  2:137438/1‑149 (MQ=255)
                                                                                                            tttGTTGAGCACAACCGAAAGCCGCTCGGGATACCGGCGTTTCTGGCGATTCGGGATGCCCTGATGCGTGCACCAGAGCCTGTTACCCTGGTGGCCATCGGCCCGTTAACCAATATTGCGCTGTTACTTTCACAATGCCCGGAATGCaa                                          <  1:2462197/149‑1 (MQ=255)
                                                                                                            tttGTTGAGCACAACCGAAAGCCGCTCGGGATACCGGCGTGTCTGGCGATTCGGGATGCCCTGATGCGTGCACCAGAGCCTGTTACCCTGGTGGCCATCGGCCCGTTAACCAATAGTGCGCTGTTACTTGCACAATGCCCGGAATGCaa                                          <  2:1888940/149‑1 (MQ=255)
                                                                                                                ttgAGCACAACCGAAAGCCGCTCGGGATACCGGCGTTTCTGGCGATTCGGGATGCCCTGATGCGTGCACCAGAGCCTGTTACCCTGGTGGCCATCGGCCCGTTAACCAATATTTCGCTGTTACTTTCACAAAGCCCGGAAAGCAAGCCg                                      >  2:4283837/1‑149 (MQ=255)
                                                                                                                   aGCACAACCGAAAGCCGCTCGGGATACCGGCGTTTCTGGCGATTCGGGATGCCCTGATGCGTGCACCAGAGCCTGTTACCCTGGTGGCCATCGGCCCGTTAACCAATATTGCGCTGTTACTTTCACAATGCCCGGAATGCAAGCCGtat                                   <  1:1096864/149‑1 (MQ=255)
                                                                                                                   aGCACAACCGAAAGCCGCTCGGGATACCGGCGTTTCTGGCGATTCGGGATGCCCTGATGCGTGCACCAGAGCCTGTTACCCTGGTGGCCATCGGCCCGTTAACCAATATTGCGCTGTTACTTTCACAATGCCCGGAATGCAAGCCGtat                                   <  1:1097188/149‑1 (MQ=255)
                                                                                                                    gCACAACCGAAAGCCGCTCGGGATACCGGCGTTTCTGGCGATTCGGGATGCCCTGATGCGTGCACCAGAGCCTGTTACCCTGGTGGCCATCGGCCCGTTAACCAATATTGCGCTGTTACTTTCACAATGCCCGGAATGCAAGCCGtata                                  <  1:5082686/149‑1 (MQ=255)
                                                                                                                    gCACAACCGAAAGCCGCTCGGGATACCGGCGTTTCTGGCGATTCGGGATGCCCTGATGCGTGCACCAGAGCCTGTTACCCTGGTGGCCATCGGCCCGTTAACCAATATTGCGCTGTTACTTTCACAATGCCCGGAATGCAAGCCGtata                                  <  1:805015/149‑1 (MQ=255)
                                                                                                                    gCACAACCGAAAGCCGCTCGGGATACCGGCGTTTCTGGCGATTCGGGATGCCCTGATGCGTGCACCAGAGCCTGTTACCCTGGTGGCCATCGGCCCGTTAACCAATATTGCGCTGTTACTTTCACAATGCCCGGAATGCAAGCCGtata                                  <  2:1338969/149‑1 (MQ=255)
                                                                                                                    gCACAACCGAAAGCCGCTCGGGATACCGGCGTTTCTGGCGATTCGGGATGCCCTGATGCGTGCACCAGAGCCTGTTACCCGGGTGGCCATCGGCCCGTTAACCAATATTGCGCTGTTACTTTCACAATGCCCCGAATGCAAGCCCtata                                  >  2:4905563/1‑149 (MQ=255)
                                                                                                                       caACCGAAAGCCGCTCGGGATACCGGCGTTTCTGGCGATTCGGGATGCCCTGATGCGTGCACCAGAGCCTGTTACCCTGGTGGCCATCGGCCCGTTAACCAATATTGCGCTGTTACTTTCACAATGCCCGGAATGCAAGCCGTATATTc                               <  2:4649787/149‑1 (MQ=255)
                                                                                                                       caACCGAAAGCCGCTCGGGATACCGGCGTTTCTGGCGATTCGGGATGCCCAGATGCGTGCACCAGAGCCTGTTACCCTGGTGGCCATCGGCCCGTTAACCAATATTGCGCTGTTACTTTCACAATGCCCGGAATGCAAGCCGTATATTc                               >  2:2005501/1‑149 (MQ=255)
                                                                                                                          ccGAAAGCCGCTCGGGATACCGGCGTTTCTGGCGATTCGGGATGCCCTGATGCGTGCACCAGAGCCTGTTACCCTGGTGGCCATCGGCCCGTTAACCAATATTGCGCTGTTACTTTCACAATGCCCGGAATGCAAGCCGTATATTCGcc                            <  2:3818989/149‑1 (MQ=255)
                                                                                                                             aaaGCCGCTCGGGATACCGGCGTTTCTGGCGATTCGGGATGCCCTGATGCGTGCACCAGAGCCTGTTACCCTGGTGGCCATCGGCCCGGTAACCAATATTTCGCTGTTACTTTCACAATGCCCGGAATGCAAGCCGTATATTCGCCGTc                         >  2:2679410/1‑149 (MQ=255)
                                                                                                                             aaaGCCGCTCGGGATACCGGCGTTTCTGGCGATTCGGGATGCCCAGATTCGTGCACCAGAGCCTGTTACCCTGGTGGCCATCGGCCCGTTAACCAATAATGCGCAGTTACTTTAACAAATACCGGAATGCAAGCCGTATATTCGCCGTc                         >  2:5144760/1‑149 (MQ=255)
                                                                                                                               aGCCGCTCGGGATACCGGCGTTTCTGGCGATTCGGGATGCCCTGATGCGTGCACCAGAGCCTGTTACCCTGGTGGCCATCGGCCCGTTAACCAATATTGCGCTGTTACTTTCACAATGCCCGGAATGCAAGCCGTATATTCGCCGTCTg                       <  2:685888/149‑1 (MQ=255)
                                                                                                                                 ccGCTCGGGATACCGGCGTTTCTGGCGATTCGGGATGCCCTGATGCGTGCACCAGAGCCTGTTACCCTGGTGGCCATCGGCCCGTTAACCAATATTGCGCTGTTACTTTCACAATGCCCGGAATGCAAGCCGTATATTCGCCGTCTGGt                     <  1:3402864/149‑1 (MQ=255)
                                                                                                                                  cGCTCGGGATACCGGCGTTTCTGGCGATTCGGGATGCCCTGATGCGTGCACCAGAGCCTGTTACCCTGGTGGCCATCGGCCCGTTAACCAATATTGCGCTGTTACTTTCACAATGCCCGGAATGCAAGCCGTATATTCGCCGTCTGGTg                    <  2:1041060/149‑1 (MQ=255)
                                                                                                                                  cGCTCGGGATACCGGCGTTTCTGGCGATTCGGGATGCCCTGATGCGTGCACCAGAGCCTGTTACCCTGGTGGCCATCGGCCCGTTAACCAATATTGCGCTGTTACTTTCACAATGCCCGGAATGCAAGCCGTATATTCGCCGTCTGGTg                    <  1:4730300/149‑1 (MQ=255)
                                                                                                                                   gCTCGGGATACCGGCGTTTCTGGCGATTCGGGATGCCCTGATGCGTGCACCAGAGCCTGTTACCCTGGTGGCCATCGGCCCGTTAACCAATATTGCGCTGTTACTTTCACAATGCCCGGAATGCAAGCCGTATATTCGCCGTCTGGTGa                   <  1:4246195/149‑1 (MQ=255)
                                                                                                                                   gCTCGGGATACCGGCGTTTCTGGCGATTCGGGATGCCCTGATGCGTGCACCAGAGCCTGTTACCCTGGTGGCCATCGGCCCGTTAACCAATATTGCGCTGTTACTTTCACAATGCCCGGAATGCAAGCCGTATATTCGCCGTCTGGTGa                   <  2:2900380/149‑1 (MQ=255)
                                                                                                                                   gCTCGGGATACCGGCGTTTCTGGCGATTAGGGATTCCCAGATTCGTGCACCACAGCCTGTTAACCTGGTGGCCCACGGCCCCTTAACCAAAATTGCGCTGGTACTTTAACAAAGCCCGGAATGCAAGCCGGATATTAGCCGTCGGGTGa                   >  2:3918587/1‑149 (MQ=255)
                                                                                                                                   gCTCGGGATACCGGCGTTTATGGCGATTCGGGATGCCCTGATGCGTGCACCAGAGCCTGTTACCCTGGTGGCCATCGGCCCGGTAACCAATATTGCGCTGTTACTTTCACAATGCCCGGAATGCAAGCCGTATATTCGCCGTCTGGTGa                   >  2:2958019/1‑149 (MQ=255)
                                                                                                                                    cTCGGGATACCGGCGTTTCTGGCGATTCGGGATGCCCTGATGCGTGCACCAGAGCCTGTTACCCTGGTGGCCATCGGCCCGTTAACCAATATTGCGCTGTTACTTTCACAATGCCCGGAATGCAAGCCGTATATTCGCCGTCTGGTGAt                  <  1:3443793/149‑1 (MQ=255)
                                                                                                                                     tCGGGATACCGGCGTTTCTGGCGATTCGGGATGCCCTGATGCGTGCACCAGAGCCTGTTACCCTGGTGGCCATCGGCCCGTTAACCAATATTGCGCTGTTACTTTCACAATGCCCGGAATGCAAGCCGTATATTCGCCGTCTGGTGATc                 <  1:1539673/149‑1 (MQ=255)
                                                                                                                                             ccGGCGTTTCTGGCGATTCGGGATGCCCTGATGCGTGCACCAGAGCCTGTTACCCTGGTGGCCATCGGCCCGTTAACCAATATTGCGCTGTTACTTTCACAATGCCCGGAATGCAAGCCGTATATTCGCCGTCTGGTGATCATGggtgg         <  2:1680188/149‑1 (MQ=255)
                                                                                                                                                gCGATTCTGGCGAGTCGGGATGCCCTGATGCGTGCACCAGAGCCTGTTACCCTGGTGGCCATCGGCCCGTTAACCAATATTGCGCTGTTACTTTCACAATGCCCGGAATGCAAGCCGTATATTCGCCGTCTGGTGATCATGGGTGGTTc      <  1:34645/149‑1 (MQ=255)
                                                                                                                                                  gTTTCTGGCGATTCGGGATGCCCTGATGCGTGCACCAGAGCCTGTTACCCTGGTGGCCATCGGCCCGTTAACCAATATTGCGCTGTTACTTTCACAATGCCCGGAATGCAAGCCGTATATTCGCCGTCTGGTGATCATGGGTGGTTCTg    >  1:2134484/1‑149 (MQ=255)
                                                                                                                                                   tttCTGGCGATTCGGGATGCCCTGATGCGTGCACCAGAGCCTGTTACCCTGGTGGCCATCGGCCCGTTAACCAATATTGCGCTGTTACTTTCACAATGCCCGGAATGCAAGCCGTATATTCGCCGTCTGGTGATCATGGGTGGTTCTGc   >  2:5042563/1‑149 (MQ=255)
                                                                                                                                                   tttCTGGCGATTCGGGATGCCCTGATGCGTGCACCAGAGCCTGTTACCCTGGTGGCCATCGGCCCGTTAACCAATATTGCGCTGTTACTTTCACAATGCCCGGAATGCAAGCCGTATATTCGCCGTCTGGTGATCATGGGTGGTTCTGc   <  1:3000752/149‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
TTCTGGAATGCGGAGATTCCGCTCGCCCAAGGGGCCGCTGTGCCACTGGTACGCGCACCGCGTGATGCGGCATCTGTGCACGGCGAATCGGGAATGGCTGGCTACGACTTTGTTGAGCACAACCGAAAGCCGCTCGGGATACCGGCGTTTCTGGCGATTCGGGATGCCCTGATGCGTGCACCAGAGCCTGTTACCCTGGTGGCCATCGGCCCGTTAACCAATATTGCGCTGTTACTTTCACAATGCCCGGAATGCAAGCCGTATATTCGCCGTCTGGTGATCATGGGTGGTTCTGCC  >  CP009273/27455‑27751

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑