Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,546,300 G→T 16.6% intergenic (‑468/‑144) malP ← / → malT maltodextrin phosphorylase/mal regulon transcriptional activator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,546,3000GT16.6% 645.7 / 27.0 237intergenic (‑468/‑144)malP/malTmaltodextrin phosphorylase/mal regulon transcriptional activator
Reads supporting (aligned to +/- strand):  ref base G (49/140);  new base T (46/2);  total (95/142)
Fisher's exact test for biased strand distribution p-value = 6.66e-20
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 241 total aligned reads displayed.

AATAGATTTATTAAGATCATCTTTTTAGATGGCACTTTCATCAGGAATGAAGAAGAAACCCTTGCTTAAATGAATCTGATGAACATAAGGGAAACCAGTATTCACGCTGGATCAGCGTCGTTTTAGGTGAGTTGTTAATAAAGATTTGGAATTGTGACACAGTGCAAATTCAGACACATAAAAAAACGTCATCGCTTGCATTAGAAAGGTTTCTGGCCGACCTTATAACCATTAATTACGAAGCGCAAAAAAAATAATATTTCCTCATTTTCCACAGTGAAGTGATTAACTATGCT  >  CP009273/3546153‑3546448
                                                                                                                                                   |                                                                                                                                                    
    gATTTATTAAGATCATCTTTTTAGATGGCACTTTCATCAGGAATGAAGAAGAAACCCTTGCTTAAATGAATCTGATGAACATAAGGGAAACCAGTATTCACGCTGGATCAGCGTCGTTTTAGGTGAGTTGTTAATAAAGATTTGGAAtt                                                                                                                                                 <  2:4505763/149‑1 (MQ=255)
    gATTTATTAAGATCATCTTTTTAGATGGCACTTTCATCAGGAATGAAGAAGAAACCCTTGCTTAAATGAATCTGATGAACATAAGGGAAACCAGTATTCACGCTGGATCAGCGTCGTTTTAGGTGAGTTGTTAATAAAGATTTGGAAtt                                                                                                                                                 <  1:2041523/149‑1 (MQ=255)
     aTTTATTAAGATCATCTTTTTAGATGGCACTTTCATCAGGAATGAAGAAGAAACCCTTGCTTAAATGAATCTGATGAACATAAGGGAAACCAGTATTCACGCTGGATCAGCGTCGTTTTAGGTGAGTTGTTAATAAAGATTTGGAATtg                                                                                                                                                <  2:3254932/149‑1 (MQ=255)
     aTTTATTAAGATCATCTTTTTAGATGGCACTTTCATCAGGAATGAAGAAGAAACCCTTGCTTAAATGAATCTGATGAACATAAGGGAAACCAGTATTCACGCTGGATCAGCGTCGTTTTAGGTGAGTTGTTAATAAAGATTTGGAATtg                                                                                                                                                <  2:4324411/149‑1 (MQ=255)
      tttATTAAGATCATCTTTTTAGATGGCACTTTCATCAGGAATGAAGAAGAAACCCTTGCTTAAATGAATCTGATGAACATAAGGGAAACCAGTATTCACGCTGGATCAGCGTCGTTTTAGGTGAGTTGTTAATAAAGATTTGGAATtgt                                                                                                                                               <  2:1574809/149‑1 (MQ=255)
                tcatcTTTTTAGATGGCACTTTCATCAGGAATGAAGAAGAAACCCTTGCTTAAATGAATCTGATGAACATAAGGGAAACCAGTATTCACGCTGGATCAGCGTCGTTTTTTGTGATTTGTTAATAAATATTTTGAATTTTGAAACAGTga                                                                                                                                     >  1:4949839/1‑148 (MQ=255)
                tcatcTTTTTAGATGGCACTTTCATCAGGAATGAAGAAGAAACCCTTGCTTAAATGAATCTGATGAACATAAGGGAAACCAGTATTCACGCTGGATCAGCGTCGTTTTAGGTGAGTTGTTAATAAAGATTTGGAATTGTGTAACAGTGc                                                                                                                                     >  2:234527/1‑149 (MQ=255)
                tcatcTTTTTAGATGGCACTTTCATCAGGAATGAAGAAGAAACCCTTGCTTAAATGAATCTGATGAACATAAGGGAAACCAGTATTCACGCTGGATCAGCGTCGTTTTAGGTGAGTTGTTAATAAAGATTTGGAATTGTGACACAGTGc                                                                                                                                     <  1:229955/149‑1 (MQ=255)
                tcatcTTTTTAGATGGCACTTTCATCAGGAATGAAGAAGAAACCCTTGCTTAAATGAATCTGATGAACATAAGGGAAACCAGTATTCACGCTGGATCAGCGTCGTGTTTTGTGTGTTTTTTTTAAAAATTTTTTATTTTGTCACACTGc                                                                                                                                     >  1:4803053/1‑149 (MQ=255)
                   tcTTTTTAGATGGCACTTTCATCAGGAATGAAGAAGAAACCCTTGCTTAAATGAATCTGATGAACATAAGGGAAACCAGTATTCACGCTGGATCAGCGTCGTTTTAGGTGAGTTGTTAATAAAGATTTGGAATTGTGACACAGTGCaaa                                                                                                                                  <  1:1045658/149‑1 (MQ=255)
                    cTTTTTAGATGGCACTTTCATCAGGAATGAAGAAGAAACCCTTGCTTAAATGAATCTGATGAACATAAGGGAAACCAGTATTCACGCTGGATCAGCGTCGTGTTAGGTGAGTTGTTTATAAAAATTTGGTATTGTGACACAGGGCaaaa                                                                                                                                 >  1:2687521/1‑148 (MQ=255)
                     tttttAGATGGCACTTTCATCAGGAATGAAGAAGAAACCCTTGCTTAAATGAATCTGATGAACATAAGGGAAACCAGTATTCACGCTGGATCAGCGTCGTTTTAGGTGAGTTGTTAATAAAGATTTGGAATTGTGTCACAGTGCAAAtt                                                                                                                                >  2:2286899/1‑149 (MQ=255)
                     tttttAGATGGCACTTTCATCAGGAATGAAGAAGAAACCCTTGCTTAAATGAATCTGATGAACATAAGGGAAACCAGTATTCACGCTGGATCAGCGTCGTTTTAGGTGAGTTGTTAATAAAGATTTGGAATTGTGACACAGTGCAAAtt                                                                                                                                <  2:1760673/149‑1 (MQ=255)
                      ttttAGATGGCACTTTCATCAGGAATGAAGAAGAAACCCTTGCTTAAATGAATCTGATGAACATAAGGGAAACCAGTATTCACGCTGGATCAGCGTCGTGTTAGGTGAGGTGTTTATAAATATTTGGAATTGTGAAACACTGCaaaata                                                                                                                               >  1:4532397/1‑146 (MQ=255)
                         tAGATGGCACTTTCATCAGGAATGAAGAAGAAACCCTTGCTTAAATGAATCTGATGAACATAAGGGAAACCAGTATTCACGCTGGATCAGCGTCGTTTTAGGTGAGTTGTTAATAAAGATTTGGAATTGTGACACAGTGCAAATTCAGa                                                                                                                            <  1:800082/149‑1 (MQ=255)
                         tAGATGGCACTTTCATCAGGAATGAAGAAGAAACCCTTGCTTAAATGAATCTGATGAACATAAGGGAAACCAGTATTCACGCTGGATCAGCGTCGTTTTAGGTGAGTTGTTAATAAAGATTTGGAATTGTGACACAGTGCAAATTCAGa                                                                                                                            <  1:2666102/149‑1 (MQ=255)
                         tAGATGGCACTTTCATCAGGAATGAAGAAGAAACCCTTGCTTAAATGAATCTGATGAACATAAGGGAAACCAGTATTCACGCTGGATCAGCGTCGTTTTAGGTGAGTTGTTAATAAAGATTTGGAATTGTGACACAGTGCAAATTCAGa                                                                                                                            <  2:3266490/149‑1 (MQ=255)
                         tAGATGGCACTTTCATCAGGAATGAAGAAGAAACCCTTGCTTAAATGAATCTGATGAACATAAGGGAAACCAGTATTCACGCTGGATCAGCGTCGTTTTAGGTGAGTTGTTAATAAAGATTTGGAATTGTGACACAGTGCAAATTCAGa                                                                                                                            <  1:3321792/149‑1 (MQ=255)
                                    ttCATCAGGAATGAAGAAGAAACCCTTGCTTAAATGAATCTGATGAACATAAGGGAAACCAGTATTCACGCTGGATCAGCGTCGTTTTATGTGAGTTTTTAATAAATATTTTTTATTTTGTCACAGTGCAAATTCAGAAACATaaaaaa                                                                                                                 >  2:3044963/1‑149 (MQ=255)
                                     tcatcaGGAATGAAGAAGAAACCCTTGCTTAAATGAATCTGATGAACATAAGGGAAACCAGTATTCACGCTGGATCAGCGTCGTTTTAGGTGAGTTGTTAATAAAGATTTGGAATTGTGACACAGTGCAAATTCAGACACATaaaaaaa                                                                                                                <  2:3544625/149‑1 (MQ=255)
                                        tcaGGAATGAAGAAGAAACCCTTGCTTAAATGAATCTGATGAACATAAGGGAAACCAGTATTCACGCTGGATCAGCGTCGTTTTAGGTGAGTTGTTAATAAATATTTTGAATTGTGACACAGTGCAAAATAAGACACATAAAAAAACGt                                                                                                             >  2:3282326/1‑149 (MQ=255)
                                         caGGAATGAAGAAGAAACCCTTGCTTAAATGAATCTGATGAACATAAGGGAAACCAGTATTCACGCTGGATCAGCGTCGTTTTAGGTGAGTTGTTAATAAAGATTTGGAATTGTGACACAGTGCAAATTCAGACACATAAAAAAACGTc                                                                                                            <  2:5148785/149‑1 (MQ=255)
                                         caGGAATGAAGAAGAAACCCTTGCTTAAATGAATCTGATGAACATAAGGGAAACCAGTATTCACGCTGGATCAGCGTCGTTTTAGGTGAGTTGTTAATAAAGATTTGGAATTGTGACACAGTGCAAATTCAGACACATAAAAAAACGTc                                                                                                            <  2:2101108/149‑1 (MQ=255)
                                          aGGAATGAAGAAGAAACCCTTGCTTAAATGAATCTGATGAACATAAGGGAAACCAGTATTCACGCTGGATCAGCGTCGTTTTTGGTGAGTTGTTAATAAAGATTTGGAATTGTGACACAGTGCAAATTCAGACACAAAAAAAAACGTCa                                                                                                           >  2:3011281/1‑149 (MQ=255)
                                          aGGAATGAAGAAGAAACCCTTGCTTAAATGAATCTGATGAACATAAGGGAAACCAGTATTCACGCTGGATCAGCGTCGTTTTAGGTGTTTTGTTAATAAAGATTTTGAATTTTGACACAGTGCAAATTAAGACACAAAAAAAAACGTCa                                                                                                           >  1:1227063/1‑149 (MQ=255)
                                           ggAATGAAGAAGAAACCCTTGCTTAAATGAATCTGATGAACATAAGGGAAACCAGTATTCACGCTGGATCAGCGTCGTTTTTTGTGAGTTTTTTATAAATATTTTTAATTGTGACACATTGCTAATTAAGAAAAATAAAAAAAAGTCAt                                                                                                          >  2:2745973/1‑149 (MQ=255)
                                            gAATGAAGAAGAAACCCTTGCTTAAATGAATCTGATGAACATAAGGGAAACCAGTATTCACGCTGGATCAGCGTCGTTTTAGGTGAGTTGTTAATAAAGATTTGGAATTGTGACACAGTGCAAATTCAGACACATAAAAAAACGTCATc                                                                                                         <  1:992909/149‑1 (MQ=255)
                                                    aagaaACCCTTGCTTAAATGAATCTGATGAACATAAGGGAAACCAGTATTCACGCTGGATCAGCGTCGTTTTAGGTGAGTTGTTAATAAAGATTTGGAATTGTGACACAGTGCAAATTCAGACACATAAAAAAACGTCATCGCTTGCAt                                                                                                 <  1:2573046/149‑1 (MQ=255)
                                                     agaaACCCTTGCTTAAATGAATCTGATGAACATAAGGGAAACCAGTATTCACGCTGGATCAGCGTCGTTTTAGGTGAGTTGTTAATAAAAATTTGGAAATGTGACACAGTGCAAATTAAGACACAAAAAAAAACGACATCGCATGCAtt                                                                                                >  2:493275/1‑149 (MQ=255)
                                                      gaaACCCTTGCTTAAATGAATCTGATGAACATAAGGGAAACCAGTATTCACGCTGGATCCGCGTCGTTTTAGGTGAGTTTTTAATAAAGATTTGGAATTGTGACACAGTGCAAATTCAGACACATAAAAAAACGTCATCGCTTGCATTa                                                                                               <  1:1334152/149‑1 (MQ=255)
                                                      gaaACCCTTGCTTAAATGAATCTGATGAACATAAGGGAAACCAGTATTCACGCTGGATCAGCGTCGTTTTAGGTGAGTTGTTAATAAAGATTTGGAATTGTGACACAGTGCAAATTCAGACACATAAAAAAACGTCATCGCTTGCATTa                                                                                               <  2:1595561/149‑1 (MQ=255)
                                                      gaaACCCTTGCTTAAATGAATCTGATGAACATAAGGGAAACCAGTATTCACGCTGGATCAGCGTCGTTTTAGGTGAGTTGTTAATAAAGATTTGGAATTGTGACACAGTGCAAATTCAGACACATAAAAAAACGTCATCGCTTGCATTa                                                                                               <  1:961544/149‑1 (MQ=255)
                                                      gaaACCCTTGCTTAAATGAATCTGATGAACATAAGGGAAACCAGTATTCACGCTGGATCAGCGTCGTTTTAGGTGAGTTGTTAATAAAGATTTGGAATTGTGACACAGTGCAAATTCAGACACATAAAAAAACGTCATCGCTTGCATTa                                                                                               <  1:961503/149‑1 (MQ=255)
                                                       aaaCCCTTGCTTAAATGAATCTGATGAACATAAGGGAAACCAGTATTCACGCTGGATCAGCGTCGTGTTATGTGAGTTGTTAATAAAGATTTTGAATTGTGACACAGTGCAAATTAAGACACAAAAAAAAAAGTCAACGCTTGCATTat                                                                                              >  2:2251969/1‑148 (MQ=255)
                                                            cTTGCTTAAATGAATCTTATGAACATAAGGGAAACCAGTATTCACGCTGGATCAGCTTCTTTTTAGGTGTGTTGTTAATAAAGATTTGGAATTGTTACACATTGCAAATTCAGACACATAAAAAAACGTCATCGCTTGCATTAGAAAgg                                                                                         <  1:1448428/149‑1 (MQ=255)
                                                            cTTGCTTAAATGAATCTGATGAACATAAGGGAAACCAGTATTCACGCTGGATCAGCGTCGTTTTAGGTGAGTTGTTAATAAAGATTTGGAATTGTGACACAGTGCAAATTCAGACACATAAAAAAACGTCATCGCTTGCATTAGAAAgg                                                                                         <  1:5016179/149‑1 (MQ=255)
                                                             ttGCTTAAATGAATCTGATGAACATAAGGGAAACCAGTATTCACGCTGGATCAGCGTCGTTTTAGGTGAGTTGTTAATAAAGATTTGGAATTGTGACACAGTGCAAATTCAGACACATAAAAAAACGTCATCGCTTGCATTAGAAAGGt                                                                                        <  2:338835/149‑1 (MQ=255)
                                                              tGCTTAAATGAATCTGATGAACATAAGGGAAACCAGTATTCACGCTGGATCAGCGTCGTGTTTGGTGTGTTGTTTATAAAGATTTTGTATTGTGACACAGTGCAAATTCAGTCACATAAAAAAAAGTGATAGCTTGCATTTGTAAGGtt                                                                                       >  2:1360118/1‑149 (MQ=255)
                                                                 ttAAATGAATCTGATGAACATGAGGGAAACCAGTATTCACGCTGGATCAGCGTCGTTTTAGGTGAGTTGTTAATAAAGATTTGGAATTGTGACACAGTGCAAATTCAGACACATAAAAAAACGTCATCGCTTGCATTAGAAAGGTTTCt                                                                                    <  2:4784315/149‑1 (MQ=255)
                                                                 ttAAATGAATCTGATGAACATAAGGGAAACCAGTATTCACGCTGGATCAGCGTCGTGTTTGGTGTGTTGTTTATAAAGATTTTTAATTGTGGCACAGTGCAAAATAAGAAACCTAAAAAAACGTCATCGCATGCATTAGAAAGGtttgt                                                                                    >  1:819639/1‑147 (MQ=255)
                                                                  tAAATGAATCTGATGAACATAAGGGAAACCAGTATTCACGCTGGATCAGCGTCGTTTTAGGTGAGTTGTTAATAAAGATTTTGTATTGTGACACAGTGCAAATTAAGACACATAAAAAAAAGTCATCGCTTGGATTAGTAAGGTTTATg                                                                                   >  2:1532920/1‑149 (MQ=255)
                                                                       gAATCTGATGAACATAAGGGAAACCAGTATTCACGCTGGATCAGCGTCGTTTTAGGTGAGTTGTTAATAAAGATTTGGAATTGTGACACAGTGCAAATTCAGACACATAAAAAAACGTCATCGCTTGCATTAGAAAGGTTTCTGGCCGa                                                                              <  1:240218/149‑1 (MQ=255)
                                                                         aTCTGATGAACATAAGGGAAACCAGTATTCACGCTGGATCAGCGTCGTTGTTGGTGTGTTGGTTATAAATATTTGGTATTGTGTCACAGGGCAAATTCAGACACAAAAAAAAAAGTCATCGCGTGCATTATAAAGGTTTATGGCCGAcc                                                                            >  1:1583443/1‑149 (MQ=255)
                                                                           cTGATGAACATAAGGGAAACCAGTATTCACGCTGGATCAGCGTCGTTTTAGGTGAGTTGTTAATAAAGATTTGGAATTGTGACACAGTGCAAATTCAGACACATAAAAAAACGTCATCGCTTGCATTAGAAAGGTTTCTGGCCGACCtt                                                                          <  1:2764599/149‑1 (MQ=255)
                                                                            tgatgaACATAAGGGAAACCAGTATTCACGCTGGATCAGCGTCGTTTTAGGTGAGTTGTTAATAAAGATTTGGAATTGTGACACAGTGCAAATTCAGACACATAAAAAAACGTCATCGCTTGCATTAGAAAGGTTTCTGGCCGACCTta                                                                         <  1:2371863/149‑1 (MQ=255)
                                                                              atgaACATAAGGGAAACCAGTAGTCACGCTGGATCAGCGTCGTTTTAGGTGAGTTGTTAATAAAGATTTGGAATTGTGACACAGTGCAAATTCAGACACATAAAAAAACGTCATCGCTTGCATTAGAAAGGTTTCTGGCCGACCTtata                                                                       <  2:4255943/149‑1 (MQ=255)
                                                                               tgaACATAAGGGAAACCAGTATTCACGCTGGATCAGCGTCGTTTTAGGTGGGTTGTTAATAAAGATTTGGAATTGTGACACAGTGCAAATTCAGACACATAAAAAAACGTCATCGCTTGCATTAGAAAGGTTTCTGGCCGACCTTATaa                                                                      >  2:307852/1‑149 (MQ=255)
                                                                               tgaACATAAGGGAAACCAGTATTCACGCTGGATCAGCGTCGTTTTAGGTGAGTTTTTAATAAAGATTTGGAATTTTGACACAGTGCAAATTAAGACCCAAAAAAAAAAGTAAACGCTTGCATTATAAATGTTTCTGGCCGACCTTATaa                                                                      >  2:1427778/1‑149 (MQ=255)
                                                                                gaACATAAGGGAAACCAGTATTCACGCTGGATCAGCGTCGTTTTAGGTGAGTTGTTAATAAAGATTTGGAATTGTGACACAGTGCAAATTCAGACACATAAAAAAACGTCATCGCTTGCATTAGAAAGGTTTCTGGCCGACCTTATAAc                                                                     <  1:2996361/149‑1 (MQ=255)
                                                                                   cATAAGGGAAACCAGTATTCACGCTGGATCAGCGTCGTTTTTGGTGAGTTGTTAATAAAGTTTTGGAATTGTGACACAGTGCAAATTAAGACACATAAAAAAAAGTCATAGCTTGCATTTGAAAGGTTTATTGCCGAACGTATAAccct                                                                  >  2:5048701/1‑147 (MQ=255)
                                                                                   cATAAGGGAAACCAGTATTCACGCTGGATCAGCGTCGTTTTAGGTGAGTTGTTAATAAAGATTTGGAATTGTGACACAGTGCAAATTCAGACACATAAAAAAACGTCATCGCTTGCATTAGAAAGGTTTCTGGCCGACCTTATAACCat                                                                  <  2:2078258/149‑1 (MQ=255)
                                                                                   cATAAGGGAAACCAGTATTCACGCTGGATCAGCGTCGTTTTAGGTGAGTTGTTAATAAAGATTTGGAATTGTGACACAGTGCAAATTCAGACACATAAAAAAACGTCATCGCTTGCATTAGAAAGGTTTCTGGCCGACCTTATAACCat                                                                  <  2:3735163/149‑1 (MQ=255)
                                                                                    aTAAGGGAAACCAGTATTCACGCTGGATCAGCGTCGTTTTAGGTGAGTTGTTAATAACGATTTGGAATTGTGACACAGTGCAAATTCAGACACATAAAAAAACGTCATCGCTTGCATTAGAAAGGTTTCTGGCCGACCTTATAACCatt                                                                 <  1:4302217/149‑1 (MQ=255)
                                                                                      aaGGGAAACCAGTATTCACGCTGGATCAGCGTCTTTTTAGGTGAGTTGTTAATAAAGATTTGGAATTGTGACACAGTGCAAATTCAGACACATAAAAAAACGTCATCGCTTGCATTAGAAAGGTTTCTGGCCGACCTTATAACCattaa                                                               <  1:4621888/149‑1 (MQ=255)
                                                                                      aaGGGAAACCAGTATTCACGCTGGATCAGCGTCGTTTTAGGTGAGTTGTTAATAAAGATTTGGAATTGTGACACAGTGCAAATTCAGACACATAAAAAAACGTCATCGCTTGCATTAGAAAGGTTTCTGGCCGACCTTATAACCattaa                                                               <  1:3846382/149‑1 (MQ=255)
                                                                                      aaGGGAAACCAGTATTCACGCTGGATCAGCGTCGTTTTAGGTGAGTTGTTAATAAAGATTTGGAATTGTGACACAGTGCAAATTCAGACACATAAAAAAACGTCATCGCTTGCATTAGAAAGGTTTCTGGCCGACCTTATAACCattaa                                                               <  1:1748651/149‑1 (MQ=255)
                                                                                       aGGGAAACCAGTATTCACGCTGGATCAGCGTCGTTTTAGGTGTGTTTTTAATAAATATTTGGTATTTTGAAACACTGCAAATTAAGACACATAAAAAAAAGTAAAAGCTTGCATTATAAAGGTTTCTGGGCGACCGTATAAACaataaa                                                              >  2:70340/1‑148 (MQ=255)
                                                                                        gggAAACCAGTATTCACGCTGGATCAGCGTCGTTTTAGGTGAGTTGTTAATAAAGATTTGGAATTGTGACACAGTGCAAATTCAGACACATAAAAAAACGTCATCGCTTGCATTAGAAAGGTTTCTGGCCGACCTTATAACCattaatt                                                             <  1:3489854/149‑1 (MQ=255)
                                                                                         ggAAACCAGTATTCACGCTGGATCAGCGTCGTTTTAGGTGAGTTGTTAATAAAGATTTGGAATTGTGACACAGTGCAAATTCAGACACATAAAAAAACGTCATCGCTTGCATTAGAAAGGTTTCTGGCCGACCTTATAACCattaatta                                                            <  2:4454963/149‑1 (MQ=255)
                                                                                         ggAAACCAGTATTCACGCTGGATCAGCGTCGTTTTAGGTGAGTTGTTAATAAAGATTTGGAATTGTGACACAGTGCAAATTCAGACACATAAAAAAACGTCATCGCTTGCATTAGAAAGGTTTCTGGCCGACCTTATAACCattaatta                                                            <  1:4507257/149‑1 (MQ=255)
                                                                                         ggAAACCAGTATTCACGCTGGATCAGCGTCGTGTTAGGTGAGTTGTTAATAAAGATTTGGTATTGTGTCACAGTGGCAAATCAGACACATAAAAAAAAGTCAAAGCGTGCATTAGAAAGGGTTATGGGCGCCCGTATAACCaataattt                                                            >  2:479574/1‑148 (MQ=255)
                                                                                          gAAACCAGTATTCACGCTGGATCAGCGTCGTTTTAGGTGAGTTGTTAATAAAGATTTGGAATTGTGACACAGTGCAAATTCAGACACATAAAAAAACGTCATCGCTTGCATTAGAAAGGTTTCTGGCCGACCTTATAACCATTAATTAc                                                           <  1:4145695/149‑1 (MQ=255)
                                                                                            aaCCAGTATTCACGCTGGATCAGCGTCGTTTTAGGTGTGTTGTTAATAAATATTTTGAAATTTGACACACTGCAAATTAAGACACAAAAAAAAACATCAACGCTTGCATTAGAAAGGTTTATTGGCGACCATATAACCattaattaagg                                                         >  2:4026339/1‑146 (MQ=255)
                                                                                            aaCCAGTATTCACGCTGGATCAGCGTCGTTTTAGGTGAGTTGTTAATAAAGATTTGGAATTGTGACACAGTGCAAATTCAGACACATAAAAAAACGTCATCGCTTGCATTAGAAAGGTTTCTGGCCGACCTTATAACCATTAATTACGa                                                         <  1:5146592/149‑1 (MQ=255)
                                                                                            aaCCAGTATTCACGCTGGATCAGCGTCGTTTTAGGTGAGTTGTTAATAAAGATTTGGAATTGTGACACAGTGCAAATTCAGACACATAAAAAAACGTCATCGCTTGCATTAGAAAGGTTTCTGGCCGACCTTATAACCATTAATTACGa                                                         <  1:2535865/149‑1 (MQ=255)
                                                                                             aCCAGTATTCACGCTGGATCAGCGTCGTTTTAGGTGAGTTGTTAATAAAGATTTGGAATTGTGACACAGTGCAAATTCAGACACATAAAAAAACGTCATCGCTTGCATTAGAAAGGTTTCTGGCCGACCTTATAACCATTAATTACGaa                                                        <  1:4397955/149‑1 (MQ=255)
                                                                                             aCCAGTATTCACGCTGGATCAGCGTCGTGTTTTGTGAGTTTTTAATAAATATTTTGAATTTTGTCAAAATGCAAAATAAGAAACAAAAAAAAAAGTAATCGCGTGCATTAGAAAGGTTTGTTGGCGCCCGTATAAACAATAATTACGaa                                                        >  1:3377171/1‑149 (MQ=255)
                                                                                                aGTATTCACGCTGGATCAGCGTCGTTTTAGGTGAGTTGTTAATAAAGATTTTGAATTGTGACACACTGCAAATTAAGACACAAAAAAAAAAGTCAAAGCATGCAATAGAAAGGTTTATTGCCGACCCTATAAACAATAATTAAGAAtcg                                                     >  2:3936371/1‑149 (MQ=255)
                                                                                                 gTATTCACGCTGGATCAGCGTCGTTTTTGGTGTGGTGTTAATAAAGATTTTGTATTTTGTCACAGTGGAAATTCAGACACAAAAAAAAAAGTCATAGCGGGCCTTAGTAAGGTTTGTGGGCGACCTTATAAACAAGAATTAAGAAtcgg                                                    >  2:4781477/1‑148 (MQ=255)
                                                                                                 gTATTCACGCTGGATCAGCGTCGTTTTAGGTGAGTTGTTAATAAAGATTTGGACTTGTGACACAGTGCAAATTCAGACACATAAAAAAACGTCATCGCTTGCATTAGAAAGGTTTCTGGCCGACCTTATAACCATTAATTACGAAgcgc                                                    <  2:2506485/149‑1 (MQ=255)
                                                                                                    ttCACGCTGGATCAGCGTCGTTTTAGGTGAGTTGTTAATAAAGATTTTGAATTGTGACACAGTGCAAATTAAGACACATAAAAAAACGTCATCGCTTGCATTAGAAAGGTTTCTGGCCGACCTTATAAACAATAAATACGAAGCGCaaa                                                 >  2:3217315/1‑149 (MQ=255)
                                                                                                      cACGCTGGATCAGCGTCGTGTTTGGTTTGTTTTTTAAAAATATTTTGTATTGTGACACAGTGCAAAATCAGACACATAAAAAAAAGTCATCGCATGCATTAGAAAGGTGTCTGGCCGACCGTATAAACAATAAATAAGAAGCGCaaaaa                                               >  1:1628559/1‑149 (MQ=255)
                                                                                                          cTGGATCAGCGTCGTTTTAGGTGAGTTGTTAATAAAGATTTGGAATTGTGACACAGTGCAAATTCAGACACATAAAAAAACGTCATCGCTTGCATTAGAAAGGTTTCTGGCCGACCTTATAACCATTAATTACGAAGCGCAAAAAAaat                                           <  1:765380/149‑1 (MQ=255)
                                                                                                               tCAGCGTCGTTTTAGGTGAGTTGTTAATAAAGATTTGGAATTGTGACACAGTGCAAATTCAGACACATAAAAAAACGTCATCGCTTGCATTAGAAAGGTTTCTGGCCGACCTTATAACCATTAATTACGAAGCGCAAAAAAAATAatat                                      <  1:1950498/149‑1 (MQ=255)
                                                                                                               tCAGCGTCGTTTTAGGTGAGTTGTTAATAAAGATTTGGAATTGTGACACAGTGCAAATTCAGACACATAAAAAAACGTCATCGCTTGCATTAGAAAGGTTTCTGGCCGACCTTATAACCATTAATTACGAAGCGCAAAAAAAATAatat                                      <  1:606019/149‑1 (MQ=255)
                                                                                                               tCAGCGTCGTTTTAGGTGAGTTGTTAATAAAGATTTGGAATTGTGACACAGTGCAAATTCAGACACATAAAAAAACGTCATCGCTTGCATTAGAAAGGTTTCTGGCCGACCTTATAACCATTAATTACGAAGCGCAAAAAAAATAatat                                      <  1:4241569/149‑1 (MQ=255)
                                                                                                                 aGCGTCGTTTTAGGTGAGTTGTTAATAAAGATTTGGAATTGTGACACAGTGCAAATTCAGACACATAAAAAAACGTCATCGCTTGCATTAGAAAGGTTTCTGGCCGACCTTATAACCATTAATTACGAAGCGCAAAAAAAATAATAttt                                    <  1:4347085/149‑1 (MQ=255)
                                                                                                                  gCGTCGTTTTAGGTGAGTTGTTAATAAAGATTTGGAATTGTGACACAGTGCAAATTCAGACACATAAAAAAACGTCATCGCTTGCATTAGAAAGGTTTCTGGCCGACCTTATAACCATTAATTACGAAGCGCAAAAAAAATAATATTTc                                   <  2:3245410/149‑1 (MQ=255)
                                                                                                                     tcgtTTTAGGTGAGTTGTTAATAAAGATTTGGAATTGTGACACAGTGCAAATTCAGACACATAAAAAAACGTCATCGCTTGCATTAGAAAGGTTTCTGGCCGACCTTATAACCATTAATTACGAAGCGCAAAAAAAATAATATTTCCTc                                <  1:1101432/149‑1 (MQ=255)
                                                                                                                     tcgtTTTAGGTGAGTTGTTAATAAAGATTTGGAATTGTGACACAGTGCAAATTCAGACACATAAAAAAACGTCATCGCTTGCATTAGAAAGGTTTCTGGCCGACCTTATAACCATTAATTACGAAGCGCAAAAAAAATAATATTTCCTc                                <  2:2437187/149‑1 (MQ=255)
                                                                                                                     tcgtTTTAGGTGAGTTGTTAATAAAGATTTGGAATTGTGACACAGTGCAAATTCAGACACATAAAAAAACGTCATCGCTTGCATTAGAAAGGTTTCTGGCCGACCTTATAACCATTAATTACGAAGCGCAAAAAAAATAATATTTCCTc                                <  2:1199916/149‑1 (MQ=255)
                                                                                                                         ttttGGTGAGTTGTTAATAAAGATTTTGAATTGTGACACAGTGCATATTCATACACATATAAATACGTCATCTCTTGCTTTAGAAAGGTTTCTGGCCGACCTTATAACCATTTATTACGATGCTCAAAAAAAATAATATTTCCTCAttt                            <  2:1632829/149‑1 (MQ=255)
                                                                                                                         tttAGGTGAGTTGTTAATAAAGATTTGGAATTGTGACACAGTGCAAATTCAGACACATAAAAAAACGTCATCGCTTGCATTAGAAAGGTTTCTGGCCGACCTTATAACCATTAATTACGAAGCGCAAAAAAAATAATATTTCCTCAttt                            <  1:92206/149‑1 (MQ=255)
                                                                                                                         tttAGGTGAGTTGTTAATAAAGATTTGGAATTGTGACACAGTGCAAATTCAGACACATAAAAAAACGTCATCGCTTGCATTAGAAAGGTTTCTGGCCGACCTTATAACCATTAATTACGAAGCGCAAAAAAAATAATATTTCCTCAttt                            <  1:3234770/149‑1 (MQ=255)
                                                                                                                         tttAGGTGAGTTGTTAATAAAGATTTGGAATTGTGACACAGTGCAAATTCAGACACATAAAAAAACGTCATCGCTTGCATTAGAAAGGTTTCTGGCCGACCTTATAACCATTAATTACGAAGCGCAAAAAAAATAATATTTCCTCAttt                            <  2:1727170/149‑1 (MQ=255)
                                                                                                                             ggTGAGTTGTTAATAAAGATTTGGAATTGTGACACAGTGCAAATTCAGACACATAAAAAAACGTCATCGCTTGCATTAGAAAGGTTTCTGGCCGACCTTATAACCATTAATTACGAAGCGCAAAAAAAATAATATTTCCTCATTTTCca                        <  1:891350/149‑1 (MQ=255)
                                                                                                                               tGAGTTGTTAATAAAGATTTGGAATTGTGACACAGTGCAAATTCAGACACATAAAAAAACGTCATCGCTTGCATTAGAAAGGTTTCTGGCCGACCTTATAACCATTAATTACGAAGCGCAAAAAAAATAATATTTCCTCATTTTCcaca                      <  2:2721304/149‑1 (MQ=255)
                                                                                                                                  gttgttAATAAAGATTTGGAATTGTGACACAGTGCAAATTCAGACACATAAAAAAACGTCATCGCTTGCATTAGAAAGGTTTCTGGCCGACCTTATAACCATTAATTACGAAGCGCAAAAAAAATAATATTTCCTCATTTTCCACagtg                   >  2:734900/1‑149 (MQ=255)
                                                                                                                                   ttgttAATAAAGATTTGGAATTGTGACACAGTGCAAATTCAGACACATAAAAAAACGTCATCGCTTGCATTAGAAAGGTTTCTGGCCGACCTTATAACCATTAATTACGAAGCGCAAAAAAAATAATATTTCCTCATTTTCCACagtga                  <  2:2272632/149‑1 (MQ=255)
                                                                                                                                     gttAATAAAGATTTGGAATTGTGACACAGTGCAAATTCAGACACATAAAAAAACGTCATCGCTTGCATTAGAAAGGTTTCTGGCCGACCTTATAACCATTAATTACGAAGCGCAAAAAAAATAATATTTCCTCATTTTCCACagtgaag                <  1:3985245/149‑1 (MQ=255)
                                                                                                                                      ttAATAAAGATTTGGAATTGTGACACAGTGCAAATTCAGACACATAAAAAAACGTCATCGCTTGCATTAGAAAGGTTTCTGGCCGACCTTATAACCATTAATTACGAAGCGCAAAAAAAATAATATTTCCTCATTTTCCACagtgaagt               >  2:4681032/1‑149 (MQ=255)
                                                                                                                                       taataaAGATTTGGAATTGTGACACAGTGCAAATTCAGACACATAAAAAAACGTCATCGCTTGCATTAGAAAGGTTTCTGGCCGACCTTATAACCATTAATTACGAAGCGCAAAAAAAATAATATTTCCTCATTTTCCACagtgaagtg              <  1:4970367/149‑1 (MQ=255)
                                                                                                                                        aataaAGATTTGGAATTGTGACACAGTGCAAATTCAGACACATAAAAAAACGTCATCGCTTGCATTAGAAAGGTTTCTGGCCGACCTTATAACCATTAATTACGAAGCGCAAAAAAAATAATATTTCCTCATTTTCCACagtgaagtga             >  2:1792248/1‑149 (MQ=255)
                                                                                                                                         ataaAGATTTGGAATTGTGACACAGTGCAAATTCAGACACATAAAAAAACGTCATCGCTTGCATTAGAAAGGTTTCTGGCCGACCTTATAACCATTAATTACGAAGCGCAAAAAAAATAATATTTCCTCATTTTCCACAGTGAAGTGAt            <  2:795315/149‑1 (MQ=255)
                                                                                                                                          taaAGATTTGGAATTGTGACACAGTGCAAATTCAGACACATAAAAAAACGTCATCGCTTGCATTAGAAAGGTTTCTGGCCGACCTTATAACCATTAATTACGAAGCGCAAAAAAAATAATATTTCCTCATTTTCCACAGTGAAGTGAtt           <  2:1913103/149‑1 (MQ=255)
                                                                                                                                          taaAGATTTGGAATTGTGACACAGTGCAAATTCAGACACATAAAAAAACGTCATCGCTTGCATTAGAAAGGTTTCTGGCCGACCTTATAACCATTAATTACGAAGCGCAAAAAAAATAATATTTCCTCATTTTCCACAGTGAAGTGAtt           >  2:3853801/1‑149 (MQ=255)
                                                                                                                                            aaGATTTTGAATTGTGACACAGTGCAAATTCAGACACATAAAAAAACGTCATCGCTTGCATTAGAAAGGTTTCTGGCCGACCTTATAACCATTAATTACGAAGCGCAAAAAAAATAATATTTCCTCATTTTCCACAGTGAAGTGATTaa         <  1:3970195/149‑1 (MQ=255)
                                                                                                                                             aGATTTGGAATTGTGACACAGTGCAAATTCAGACACATAAAAAAACGTCATCGCTTGCATTAGAAAGGTTTCTGGCCGACCTTATAACCATTAATTACGAAGCGCAAAAAAAATAATATTTCCTCATTTTCCACAGTGAAGTGATTAAc        <  1:2972912/149‑1 (MQ=255)
                                                                                                                                                   ggAATTGTGACACAGTGCAAATTCAGACACATAAAAAAACGTCATCGCTTGCATTAGAAAGGTTTCTGGCCGACCTTATAACCATTAATTACGAAGCGCAAAAAAAATAATATTTCCTCATTTTCCACAGTGAAGTGATTAACTATGCt  <  2:3759700/149‑1 (MQ=255)
                                                                                                                                                   ggAATTGTGACACAGTGCAAATTCAGACACATAAAAAAACGTCATCGCTTGCATTAGAAAGGTTTCTGGCCGACCTTATAACCATTAATTACGAAGCGCAAAAAAAATAATATTTCCTCATTTTCCACAGTGAAGTGATTAACTATGCt  <  1:4463286/149‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                    
AATAGATTTATTAAGATCATCTTTTTAGATGGCACTTTCATCAGGAATGAAGAAGAAACCCTTGCTTAAATGAATCTGATGAACATAAGGGAAACCAGTATTCACGCTGGATCAGCGTCGTTTTAGGTGAGTTGTTAATAAAGATTTGGAATTGTGACACAGTGCAAATTCAGACACATAAAAAAACGTCATCGCTTGCATTAGAAAGGTTTCTGGCCGACCTTATAACCATTAATTACGAAGCGCAAAAAAAATAATATTTCCTCATTTTCCACAGTGAAGTGATTAACTATGCT  >  CP009273/3546153‑3546448

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑