Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,721,485 T→G 11.9% Y416S (TAC→TCC)  xylB ← xylulokinase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,721,4850TG11.9% 613.4 / 19.1 248Y416S (TAC→TCC) xylBxylulokinase
Reads supporting (aligned to +/- strand):  ref base T (78/134);  new base G (33/3);  total (111/137)
Fisher's exact test for biased strand distribution p-value = 3.17e-10
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 252 total aligned reads displayed.

AAGCGGCATAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCATCTGCCAGCGCATAACCCACGCCTTC  >  CP009273/3721339‑3721633
                                                                                                                                                   |                                                                                                                                                    
aaGCGGCATAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAG‑CCCCCCCCGTCCGGtca                                                                                                                                                    >  1:3103883/1‑147 (MQ=255)
       aTAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACCT‑CGCCCCCCGTCCGGTCTTCGAgct                                                                                                                                             >  2:4123930/1‑149 (MQ=255)
       aTAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCCGTGCGGTGATCGCgct                                                                                                                                             >  2:1510579/1‑149 (MQ=255)
       aTAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCCGTACGGTAATCGAgct                                                                                                                                             <  2:4900744/149‑1 (MQ=255)
        tAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTTGTTGCGGCAACTATTCACTGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCCGTACGGTAATCGAgctg                                                                                                                                            <  2:4815390/149‑1 (MQ=255)
        tAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCCGTACGGTAATCGAgctg                                                                                                                                            <  2:3338825/149‑1 (MQ=255)
          aCGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCCGTACGGTAATCGAgctgct                                                                                                                                          <  1:563866/149‑1 (MQ=255)
               gcgcATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCCGGCCGGGACTCGAGCGGCGGTCCg                                                                                                                                     >  1:3143578/1‑149 (MQ=255)
                cgcATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGc                                                                                                                                    <  2:2199048/149‑1 (MQ=255)
                cgcATCTGGTAGATGCGACTGTTCCAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCCGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCCCCG‑CCCCCCCCGGACGGTCAGCGGGCGGCGGGCCGc                                                                                                                                    >  2:4425945/1‑149 (MQ=255)
                  cATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTg                                                                                                                                  <  2:85437/149‑1 (MQ=255)
                   aTCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGa                                                                                                                                 <  1:5180786/149‑1 (MQ=255)
                     cTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGata                                                                                                                               <  2:4835792/149‑1 (MQ=255)
                     cTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCCGGACGGTAATCGAGCTGCTGACCGCTGtta                                                                                                                               >  1:2501079/1‑149 (MQ=255)
                             tGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCCGTACGGTACTCGAGCTGCTGGGCGCTGAGATCCGcccg                                                                                                                       >  2:2131485/1‑147 (MQ=255)
                                   tGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCCGTCCGGGAGTCGAGCTGCGGACCGCGGATATCCGCCAGCATCTg                                                                                                                 >  1:2012934/1‑149 (MQ=255)
                                    gTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGa                                                                                                                <  1:5012325/149‑1 (MQ=255)
                                    gTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGa                                                                                                                <  1:4724855/149‑1 (MQ=255)
                                    gTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGa                                                                                                                <  1:3365675/149‑1 (MQ=255)
                                    gTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCCGTACGGGCGTCGCGCGGCTGACCGCGGGTAGCCGGCGGCAGCTgc                                                                                                                >  2:4352114/1‑148 (MQ=255)
                                        tAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCCGTACGGTAGTCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGTCGcc                                                                                                            >  2:1089910/1‑149 (MQ=255)
                                        tAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGcc                                                                                                            <  1:4399115/149‑1 (MQ=255)
                                         aaCGGTAGTTGCGGCAACAATTCAATGAGCGCTTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCa                                                                                                           <  2:3180831/149‑1 (MQ=255)
                                         aaCGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCCGGACGGGGATCGAGCTGGTGTCCGCTGGTCTCCGCCAGCGTCTGACGCCa                                                                                                           >  1:3189665/1‑149 (MQ=255)
                                          aCGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAg                                                                                                          <  2:1550219/149‑1 (MQ=255)
                                            ggTAGTTGCGGCAACAAGTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTa                                                                                                        <  2:4530611/149‑1 (MQ=255)
                                             gTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCCGGACGGTACTCGAGCTGCTGACCGCTGGGATCCGCCCGCATCTGGCGCCAGTAc                                                                                                       >  1:1878425/1‑149 (MQ=255)
                                              tAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACt                                                                                                      <  1:1040271/149‑1 (MQ=255)
                                                gTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCCCAG‑CCCCCCCCGGGCGGTAATCGTGCTGCTGGCCGGTGTTATGCGCGAGCTTGTGGCGCCAGTAcggg                                                                                                    >  2:2517766/1‑146 (MQ=255)
                                                   gCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCCGTGCGGTACTCGTGCTGCTGGCCGCTGATATGCGGCCGCGTCTGGCGCCAGTACTCaggg                                                                                                 >  2:626746/1‑146 (MQ=255)
                                                   gCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTa                                                                                                 <  2:34906/149‑1 (MQ=255)
                                                      gCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTAcgc                                                                                              <  1:1261716/149‑1 (MQ=255)
                                                      gCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAG‑CCCCCCCCGTGCGGTAATCGTGCTGCTGGCCGCGGGTATCCGCCCGCCTCTGACGCCCGTCCTCTCTAcgc                                                                                              >  1:4514496/1‑149 (MQ=255)
                                                         acaaTTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCCGTACGGGCATCGAGCTGCGGACCGCTGCGATCCGCCAGCATCTGACGCGCGTCGTCCCTACGcgcc                                                                                           >  1:4061743/1‑149 (MQ=255)
                                                           aaTTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCCGTACGGTAATCGGGCTGCTGACCGCGGATATCCGCCAGCATCTGACGCCAGTACTCACTCCGcgcccc                                                                                         >  1:1457395/1‑149 (MQ=255)
                                                           aaTTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGcgcccc                                                                                         <  2:3779184/149‑1 (MQ=255)
                                                               cAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGcgccccgccc                                                                                     <  1:1474580/149‑1 (MQ=255)
                                                                 aTGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGccccc                                                                                   <  1:2977623/149‑1 (MQ=255)
                                                                 aTGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGccccc                                                                                   <  1:1179398/149‑1 (MQ=255)
                                                                 aTGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGccccc                                                                                   <  1:390006/149‑1 (MQ=255)
                                                                  tGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCCGTCCGGGCCTCGCGCTGCGGACCGCTGATATCCGCCCGCCTCTGACGCCAGGACTCACTACGCGCCCCGcccccc                                                                                  >  2:2938392/1‑148 (MQ=255)
                                                                   gAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCaa                                                                                 <  1:467250/149‑1 (MQ=255)
                                                                   gAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCaa                                                                                 <  2:853765/149‑1 (MQ=255)
                                                                    aGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAG‑CCCCCCCCGGACGGTATTCGAGCTGCTGACCGCGGGTGTCCGCCCGCCTCTGTCGCCAGTACGCACTGCGCGCCCCGccccccct                                                                                >  1:3934884/1‑146 (MQ=255)
                                                                     gCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATc                                                                               <  1:4039051/149‑1 (MQ=255)
                                                                        aTTTCTCTGGATTCGCCGCTATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAAc                                                                            <  2:2305936/149‑1 (MQ=255)
                                                                        aTTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCCGTACGGTCATCGAGCTGCTGACCGCGGTTATCCGCCGGCCTCTGACGCCCGTCCTCGCTACGCGCCCCGCCCCCCAGCAAc                                                                            >  2:3087460/1‑149 (MQ=255)
                                                                        aTTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCGTCTGACGCCAGTGCTCACTACGCGCCCCGCCCCCGATCAAc                                                                            >  1:1603770/1‑149 (MQ=255)
                                                                        aTTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAAc                                                                            <  2:3545472/149‑1 (MQ=255)
                                                                         tttCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCCGTCCGGGACTCGAGCTGCTGGCCGCTGATGTCCGCCAGCATCTGACGCCAGTTCGCCCTACGCGCCCCGCCCCCAATCAACg                                                                           >  1:1814140/1‑149 (MQ=255)
                                                                         tttCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGGTATCCGCCCGCATCTGACGCCAGTACGCACTAGGCGCCCCGCCCCCGATCCACg                                                                           >  2:1328884/1‑149 (MQ=255)
                                                                         tttCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACg                                                                           <  1:1402961/149‑1 (MQ=255)
                                                                          ttCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGt                                                                          <  1:3437922/149‑1 (MQ=255)
                                                                            ctctGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCCGTACGGTAATCGTGCTGCTGACCGCGGATATCCGCCCGCAGCTGACGCCAGTACGCACTACGCGCCCCGCCCCCACTCAACGGaa                                                                        >  2:3382497/1‑149 (MQ=255)
                                                                             tctGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACCT‑CCCCCCCCGGGCGGGACTCGAGCTGCGGTCCGCTGATATCCGCCAGCAGCTGACGCCAGTACTCTCTCCGCGCCCCGCCCCCCAGCAACggacc                                                                       >  2:4405850/1‑145 (MQ=255)
                                                                             tctGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCCGTCCGGGGATCGAGCTGCTGACCGCTGGTATCCGCCCGCAGCTGACGCCAGGACTCACTCCGCGCCCCGCCCCCAAGCAACGtccc                                                                       >  2:1794679/1‑146 (MQ=255)
                                                                             tctGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAac                                                                       <  1:2827114/149‑1 (MQ=255)
                                                                               tGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCGCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAacac                                                                     >  1:2098320/1‑149 (MQ=255)
                                                                                  aTTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACttt                                                                  <  2:1401645/149‑1 (MQ=255)
                                                                                    tCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGc                                                                <  1:3850157/149‑1 (MQ=255)
                                                                                    tCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGc                                                                <  1:2682041/149‑1 (MQ=255)
                                                                                     cgccgcGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCg                                                               <  1:4269126/149‑1 (MQ=255)
                                                                                     cgccgcGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCCGGACGGGAATCGAGCTGCTGACCGCTGAGATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTGCCGCTTTGCg                                                               >  1:4769873/1‑149 (MQ=255)
                                                                                        cgcgATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGtt                                                            <  1:2394532/149‑1 (MQ=255)
                                                                                         gcgATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGttt                                                           <  2:5027111/149‑1 (MQ=255)
                                                                                         gcgATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCCGGACGGGAATCGAGCTGCTGACCGCTGGGAGGCGCCCGCATCTGACGCCCGTGCTCACTGCGCGCCCCGCCCCCCAGCCCCGTAACACTTTGCggggt                                                           >  1:489812/1‑146 (MQ=255)
                                                                                           gATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCCGTGCGGTACTCGAGCTGCTGGCCGCGGTTAGCCGCCAGCAGCTGACGCCAGTACGCACTCCGCGCCCCGCCCCCCCGCAACGGGACACTTTGCGGTTTaa                                                         >  1:976217/1‑149 (MQ=255)
                                                                                            aTCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTACCTCTTTGCGGTTTCAt                                                        >  2:1612454/1‑149 (MQ=255)
                                                                                            aTCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAAt                                                        <  1:2192212/149‑1 (MQ=255)
                                                                                             tCTGCGCCAGCCTGGCTGTGCCCAGTGCTGGCCCCACCTCCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAAt                                                        <  1:2292469/149‑1 (MQ=255)
                                                                                                  gcCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCGGATATCCGCCAGCATCTGGCGCCAGTACTCACTACGCGCCCCGCCCCCACTCAACGTAACACTTTGCGGTTTAATACCGCa                                                  >  1:827963/1‑149 (MQ=255)
                                                                                                     aGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCCGTACGGTAATCGAGCTGCTGTCCGCTGATATCCGCCAGCATCTGACGCCAGGACTCACTACGCGCCCCGCCCCCAAGCAACGTAACACGTTGCGGGTTAATACCGCAGGc                                               >  2:2017526/1‑149 (MQ=255)
                                                                                                     aGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGc                                               <  1:2421623/149‑1 (MQ=255)
                                                                                                      gCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCCGTACGGTAATCGAGCTGCTGGCCGCTGTTCTCCGCCAGCATCTGTCGCCAGGACTCACTACGCGCCCCGCCCCCAATCAACGTACCACTTTGCGGTTTTATACCGCAGGCa                                              >  1:1951954/1‑149 (MQ=255)
                                                                                                      gCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCa                                              <  1:263840/149‑1 (MQ=255)
                                                                                                       ccTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCAt                                             <  1:1028261/149‑1 (MQ=255)
                                                                                                       ccTTGCTGCGCCCAGTGCTGGCCCCACAG‑CCCCCCCCGGGCGGTACGCGAGCTGCTGACCGCGGAGATCCGCCCGCAGCGGACGCCAGTACGCACTACGCGCCCCGCCCCCCCTCAACGTAACCCGTTGCGGGTTCATACCGCCGGCAt                                             >  2:3825432/1‑149 (MQ=255)
                                                                                                               cgcCCAGTGCTGGCCCCACAT‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCCATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACa                                     <  2:3217389/149‑1 (MQ=255)
                                                                                                                gcCCAGTGCTGGCCCCACAT‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGGTATCCGCCAGCATCTGACGCCCGTACGCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGGTTAATACCGCCGGCATGCCCgccct                                    >  1:3167569/1‑145 (MQ=255)
                                                                                                                 cccAGTGCTGGCCCCACAT‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATc                                   <  1:2583629/149‑1 (MQ=255)
                                                                                                                 cccAGTGCTGGCCCCACAT‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATc                                   <  1:2582602/149‑1 (MQ=255)
                                                                                                                  ccAGTGCTGGCCCCACCT‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGGTATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATcc                                  >  1:3056051/1‑149 (MQ=255)
                                                                                                                  ccAGTGCTGGCCCCACAT‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGGTATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACGTTGCGGTTTAATACCGCAGGCATGCACGACATcc                                  >  1:1812357/1‑149 (MQ=255)
                                                                                                                    aGTGCTGGCCCCACAT‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCAt                                <  2:408524/149‑1 (MQ=255)
                                                                                                                      tGCTGGCCCCACAT‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGc                              <  2:2424096/149‑1 (MQ=255)
                                                                                                                      tGCTGGCCCCACAG‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGc                              <  2:3307000/149‑1 (MQ=255)
                                                                                                                         tGGCCCCACAT‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCAt                           <  2:1148160/149‑1 (MQ=255)
                                                                                                                          ggCCCCACAT‑CCACCCCCGTACGGTAATCGAGCTTCTGAACGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACAGCCATGCCATc                          >  2:2066022/1‑149 (MQ=255)
                                                                                                                           gCCCCACAT‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCATCt                         <  2:627956/149‑1 (MQ=255)
                                                                                                                                 caT‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCATCTGCCAgc                   <  2:926739/149‑1 (MQ=255)
                                                                                                                                  aT‑CCCCCCCCGTACTGTAATCGAGCAGCAGACCCCTGAAATCCGCCAGCATATGACGCCAGTACTCACTACCCCCCCCGCCCCCAAACAAACAAACACATAGAGGTTTAATACCGCACGCATGCAAGACATCCAAGCCATCTGCCAgcc                  >  1:4144959/1‑148 (MQ=255)
                                                                                                                                  aT‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCATCTGCCAgcg                  >  2:5190661/1‑149 (MQ=255)
                                                                                                                                     ccccccccGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCACTCCACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCATCTGCCAGCGCa                <  2:5126484/149‑1 (MQ=255)
                                                                                                                                     ccccccccGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCATCTGCCAGCGCa                >  1:3773561/1‑149 (MQ=255)
                                                                                                                                       ccccccGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCATCTGCCAGCGCATa              <  1:608529/149‑1 (MQ=255)
                                                                                                                                       ccccccGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCATCTGCCAGCGCATa              <  1:605824/149‑1 (MQ=255)
                                                                                                                                                  gTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCATCTGCCAGCGCATAACCCACGCCtt   >  1:2510294/1‑149 (MQ=255)
                                                                                                                                                  gTAATCGAGCTGCTGACCGCTGATATCAGCCAGAATCTGACGCCATTACTCACTAAGCGCCTCGCCCCCAATCAACGGAACACTTTGCTGTTTAATAACGCAGGCATGCACGACATCCATGCCATCTGCCAGCGCATATCCCACGCCtt   <  2:2154088/149‑1 (MQ=255)
                                                                                                                                                   tAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCATCTGCCAGCGCATAACCCACGCCTTc  <  1:2744521/149‑1 (MQ=255)
                                                                                                                                                   tAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCATCTGCCAGCGCATAACCCACGCCTTc  >  1:3031134/1‑149 (MQ=255)
                                                                                                                                                   |                                                                                                                                                    
AAGCGGCATAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCATCTGCCAGCGCATAACCCACGCCTTC  >  CP009273/3721339‑3721633

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑