BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	3,872,511	A→C	5.6%	V323V (GTT→GTG)	gyrB ←	DNA gyrase, subunit B

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	3,872,511	0	A	C	5.6%	862.5 / 2.1	300	V323V (GTT→GTG)	gyrB	DNA gyrase, subunit B
Reads supporting (aligned to +/- strand): ref base A (173/100); new base C (2/25); total (175/125)
Fisher's exact test for biased strand distribution p-value = 9.87e-09
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 306 total aligned reads displayed.

CAACCACGATTTTCGCGTCGGTTGGGTTTTCCAGCAGGTATTCTGCCAGCAGTTCGTTCATCTGCTGTTCAACCGCCGATTTCACCTCAGAAGAAACCAGTTTGTCTTTGGTCTGGGAGGAGAATTTCGGGTCCGGCACTTTCACGGAAACGACCGCAATCAGGCCTTCACGCGCATCGTCACCGGTGGCGCTGACTTTGGCTTTTTTGCTGTAGCCTTCTTTGTCCATGTAGGCGTTCAGGGTACGGGTCATCGCCGCACGGAAGCCTGCCAGGTGAGTACCGCCGTCACGCTGCG  >  CP009273/3872363‑3872659

cAACCACGATTTTCGCGTCGGTTGGGTTTTCCAGCAGGTATTCTGCCAGCAGTTCGTTCAACAGCTTTTCAACCGCCGATTTCACCTCAGAAGAAACCAGTTTGTCTTTTGTCTGGGAGGAGAATTTCGGGTCCGGCACTTTCACGGaa                                                                                                                                                      >  1:2636749/1‑149 (MQ=255)

    cACGATTTTCGCGTCGGTTGGGTTTTCCAGCAGGTATTCTGCCAGCAGTTCGTTCATCTGCTGTTCAACCGCCGATTTCACCTCAGAAGAAACCAGTTTGTCTTTGGTCTGGGAGGAGAATTTCGGGTCCGGCACTTTCACGGAAACGa                                                                                                                                                  <  1:126082/149‑1 (MQ=255)

             cgcgTCGGTTGGGTTTTCCAGCAGGTATTCTGCCAGCAGTTCGTTCATCTGCTGTTCAACCGCCGATTTCACCTCAGAAGAAACCAGTTTGTCTTTGGTCTGGGAGGTGAATTTCGGGTCCGGCACTTTCACGGAAACGACCGCAATCa                                                                                                                                         >  1:4414881/1‑149 (MQ=255)

              gcgTCGGTTGGGTTTTCCAGCAGGTATTCTGCCAGCAGTTCGTTCATCTGCTGTTCAACCGCCGATTTCACCTCAGAAGAAACCAGTTTGTCTTTGGTCTGGGAGGAGAATTTCGGGTCCGGCACTTTCACGGAAACGACCGCAATCAg                                                                                                                                        >  2:594189/1‑149 (MQ=255)

              gcgTCGGTTGGGTTTTCCAGCAGGTATTCTGCCAGCAGTTCGTTCATCTGCTGTTCAACCGCCGATTTCACCTCAGAAGAAACCAGTTTGTCTTTGGTCTGGGAGGAGAATTTCGGGTCCGGCACTTTCACGGAAACGACCGCAATCAg                                                                                                                                        >  2:1628771/1‑149 (MQ=255)

                 tCGGTTGGGTTTTCCAGCAGGTATTCTGCCAGCAGTTCGTTCATCTGCTGTTCAACCGCCGATTTCACCTCAGAAGAAACCAGTTTGTCTTTGGTCTGGGAGGAGAATTTCGGGTCCGGCACTTTCACGGAAACGACCGCAATCAGGcc                                                                                                                                     >  1:3876409/1‑149 (MQ=255)

                 tCGGTTGGGTTTTCCAGCAGGTATTCTGCCAGCAGTTCGTTCATCTGCTGTTCAACCGCCGATTTCACCTCAGAAGAAACCAGTTTGTCTTTGGTCTGGGAGGAGAATTTCGGGTCCGGCACTTTCACGGAAACGACCGCAATCAGGcc                                                                                                                                     >  2:3730239/1‑149 (MQ=255)

                  cGGTTGGGTTTTCCAGCAGGTATTCTGCCAGCTGTTCGTTCATCTGCTGTTCAACCGCCGATTTCACCTCAGAAGAAACCTGTTTGTCTTTGGTCTGGGAGGAGAATTTCGGGTCCGGCACTTTCACGGAAACGACCGCAATCAGGCCt                                                                                                                                    <  2:3398471/149‑1 (MQ=255)

                       gggTTTTCCAGCAGGTATTCTGCCAGCAGTTCGTTCATCTGCTGTTCAACCGCCGATTTCACCTCAGAAGAAACCAGTTTGTCTTTGGTCTGGGAGGAGAATTTCGGGTCCGGCACTTTCACGGAAACGACCGCAATCAGGCCTTCAcg                                                                                                                               >  1:2905495/1‑149 (MQ=255)

                        ggTTTTCCAGCTGGTATTCTGCCAGCTGTTCGTTCATCTGCTGTTCAACCGCCGATTTCACCTCTGAAGAAACCAGTGTGTCTTTGGTCTGGGCGGAGAATTTCGGGTCCGGCACTTTCACGGAAACGACCGCAATCAGGCCTTCAcgc                                                                                                                              <  2:4630945/149‑1 (MQ=255)

                           tttCCCGCTGTTATTCGGCCAGCAGTTCGTTCATCTGCTGTTCAACCGCCTTTTTCACCTCATAATAAACCAGTTTGTCTTTGGTCTGGGAGGAGAATTTCGGGTCCGGCACTTACACGTAAACGACCGCAATCAGGCCTTCACGCGCa                                                                                                                           <  2:3102672/149‑1 (MQ=255)

                           tttCCAGCAGTTATTCTGCCAGCTGTTCGTTAATGTGGTGTTCAACCGCCGATTTCACCACACAATAACCCTGTTTGTCTTGGGTCTGGGTGGAGATTTTGGGGTCCGGCACTTTCACGGAAACGACCGCAATCAGGCCTTCACGCGCa                                                                                                                           <  2:1726422/149‑1 (MQ=255)

                           tttCCAGCAGGTATTCTGCCAGCAGTTCGTTCATCTGCTGTTCAACCGCCGATTTCACCTCAGAAGAAACCAGTTTGTCTTTGGTCTGGGAGGAGAATTTCGGGTCCGGCACTTTCACGGAAACGACCGCAATCAGGCCTTCACGCGCa                                                                                                                           >  2:181924/1‑149 (MQ=255)

                               cagcagGTATTCTGCCAGCTGTTCGTTCATCTGCTGTTCAACCGCCGTTTTCACCTCCGAAGAAACCAGTTTGTCTTTGGTCTGGGAGGAGAATTTCGGGTCCGGCACTTTCACGGAAACGACCGCAATCAGGCCTTCACGCGCATCGt                                                                                                                       <  2:5171530/149‑1 (MQ=255)

                                agcagGTATTCTGCCAGCAGTTCGTTCATCTGCTGTTCAACCGCCGATTTCACCTCAGAAGAAACCAGTTTGTCTTTGGTCTGGGAGGAGAATTTCGGGTCCGGCACTTTCACGGAAACGACCGCAATCAGGCCTTCACGCGCATCGTc                                                                                                                      >  1:1950862/1‑149 (MQ=255)

                                  ctggTTTTCTGCCAGCTGTTCGTTCATCTGTTGTTCAACCGCCGTTTTCACCTCAGAAGAAACCTGTTTGTCTTTGGTCTGGTAGTATAATTTCGGGTCCGGCACTTTCACGTAAACGACCGCAATCAGGCCTTCACGCGCATCGTCAc                                                                                                                    <  2:1741654/147‑1 (MQ=255)

                                   agGTATTCTGCCAGCAGTTCGTTCATCTGCTGTTCAACCGCCGATTTCACCTCAGAAGAAACCAGTTTGTCTTTGGTCTGGGAGGAGAATTTCGGGTCCGGCACTTTCACGGAAACGACCGCAATCAGGCCTTCACGCGCATCGTCAcc                                                                                                                   >  1:3938152/1‑149 (MQ=255)

                                    ggTATTCTGCCAGCAGTTCGTTCATCTGCTGTTCAACCGCCGATTTCACCTCAGAAGAAACCAGTTTGTCTTTGGTCTGGGAGGAGAATTTCGGGTCCGGCACTTTCACGGAAACGACCGCAATCAGGCCTTCACGCGCATCGTCACCg                                                                                                                  >  1:2927021/1‑149 (MQ=255)

                                      tATTCTGCCAGCAGTTCGTTCATCTGCTGTTCAACCGCCGATTTCACCTCAGAAGAAACCAGTTTGTCGTTGGTCTGGGAGGAGAATTTCGGGTCCGGCACTTTCACGGAAACGACCGCAATCAGGCCTTCACGCGCATCGTCACCGGt                                                                                                                >  1:51907/1‑149 (MQ=255)

                                            gCCATCTGTTCGTTCATCTGCTTTACACCCGCCGATTTCACATCAGAACAAACCTTTTTGTCTTTGGTGTGGGATTATATTTTGGGGTCCGCAACTTACACGCAAACGACCGCAATCAGGCCTTCACGCGCATCGTCACCGGTGGCGCt                                                                                                          <  1:4809279/149‑1 (MQ=255)

                                            gCCAGCAGTTCGTTCATCTGCTGTTCAACCGCCGATTTCACCTCAGAAGAAACCAGTTTGTCTTTGGTCTGGGAGGAGAATTTCGGGTCCGGCACTTTCACGGAAACGACCGCAATCAGGCCTTCACGCGCATCGTCACCGGTGGCGCt                                                                                                          >  2:755736/1‑149 (MQ=255)

                                             ccAGCAGTTCGTTCATCTGCTGTTCAACCGCCGATTTCACCTCAGAAGAAACCAGTTTGTCTTTGGTCTGGGAGGAGAATTTCGGGTCCGGCACTTTCACGGAAACGACCGCAATCAGGCCTTCACGCGCATCGTCACCGGTGGCGCTg                                                                                                         <  2:3458369/149‑1 (MQ=255)

                                              cagctgTTCGTTCATCTGCTGTTCAACCGCCGATTTCACCTCAGAAGAAACCTGTTTGTCTTTGGTCTGGGAGGAGAATTTCGGGTCCGGCACTTTCACGGAAACGACCGCAATCAGGCCTTCACGCGCATCGTCACCGGTGGCGCTGa                                                                                                        <  2:3168904/149‑1 (MQ=255)

                                              cagcagTTCGTTCATCTGCTGTTCAACCGCCGATTTCACCTCAGAAGAAACCAGTTTGTCTTTGGTCTGGGAGGAGAATTTCGGGTCCGGCACTTTCACGGAAACGACCGCAATCAGGCCTTCACGCGCATCGTCACCGGTGGCGCTGa                                                                                                        >  2:1299740/1‑149 (MQ=255)

                                               agcagTTCGTTCATCTGCTGTTCAACCGCCGATTTCACCTCAGAAGAAACCAGTTTGTCTTTGGTCTGGGAGGAGAATTTCGGGTCCGGCACTTTCACGGAAACGACCGCAATCAGGCCTTCACGCGCATCGTCACCGGTGGCGCTGAc                                                                                                       >  1:4015124/1‑149 (MQ=255)

                                                gcagTTCGTTCATCTGCTGTTCAACCGCCGATTTCACCTCAGAAGAAACCAGTTTGTCTTTGGTCTGGGAGGAGAATTTCGGGTCCGGCACTTTCACGGAAACGACCGCAATCAGGCCTTCACGCGCATCGTCACCGGTGGCGCTGACt                                                                                                      >  1:3003173/1‑149 (MQ=255)

                                                gcagTTCGTTCATCTGCTGTTCAACCGCCGATTTCACCTCAGAAGAAACCAGTTTGTCTTTGGTCTGGGAGGAGAATTTCGGGTCCGGCACTTTCACGGAAACGACCGCAATCAGGCCTTCACGCGCATCGTCACCGGTGGCGCTGACt                                                                                                      >  1:4304821/1‑149 (MQ=255)

                                                gcagTTCGTTCATCTGCTGTTCAACCGCCGATTTCACCTCAGAAGAAACCAGTTTGTCTTTGGTCTGGGAGGAGAATTTCGGGTCCGGCACTTTCACGGAAACGACCGCAATCAGGCCTTCACGCGCATCGTCACCGGTGGCGCTGACt                                                                                                      >  1:851140/1‑149 (MQ=255)

                                                 ctgttcgttcATCTGCTGTTCAACCGCCGATTTCACCTCAGAAGAAACCTGTTTGTCTTGGGTCTGGGTGGATAATTTCGGGTCCGGCACTTTCACGGAAACGACCGCAATCAGGCCTTCACGCGCATCGTCACCGGTGGCGCTGACtt                                                                                                     <  1:3318854/147‑1 (MQ=255)

                                                 catttggttcATCTGCGTTACAACCGCCTATTACCCCTCACAATAAACCATTTTGTCTTTGGTCTGGGTGGATAATTTCGGGTCCGGCACTTTCACGGAAACGACCGCAATCAGGCCTTCACGCGCATCGTCACCGGTGGCGCTGACtt                                                                                                     <  1:4281402/143‑1 (MQ=255)

                                                  tgttcgttcATCTGCTGTACAACCGCCGTTTTCACCTCAGAAGAACCCAGTTTGTCTTTGGTCTGGGAGGAGATTTTCGGGTCCGGAACTTTCACGGACACGACCGCAATCAGGCCTTCACGCGCATCGTCACCGGTGGCGCTGACttt                                                                                                    <  2:3976415/148‑1 (MQ=255)

                                                  agTTCTTTAATCTGCTTTTAACCCGCCGTTTTCACCTCAGAAGAAACCTGTTTGTCTTTGGTGTGGGTGGAGAATTTCGGGTCCGGCACTTACACGGAAACGACCGCAATCAGGCCTTCACGCGCATCGTCACCGGTGGCGCTGACttt                                                                                                    <  1:3326815/149‑1 (MQ=255)

                                                  agTTCGTTCATCTGCTGTTCAACCGCCTATTTCACCTCAGAAGAAACCAGTTTGTCTTTGGTCTGGGAGGAGAATTTCGGGTCCGGCACTTTCACGTACACGACCGCAATCAGGCCTTCACGCGCATCGTCACCGGTGGCGCTGACttt                                                                                                    <  2:2420238/149‑1 (MQ=255)

                                                  agTTCGTTCATCTGCTGTTCAACCGCCGATTTCACCTCAGAAGAAACCAGTTTGTCTTTGGTCTGGGAGGAGAATTTCGGGTCCGGCACTTTCACGGAAACGACCGCAATCAGGCCTTCACGCGCATCGTCACCGGTGGCGCTGACttt                                                                                                    >  2:1085684/1‑149 (MQ=255)

                                                    ttcgttcATCTGCTGTTCAACCGCCGATTTCACCTCAGAAGAAACCAGTTTGTCTTTGGTCTGGGAGGAGAATTTCGGGTCCGGCACTTTCACGGAAACGACCGCAATCAGGCCTTCACGCGCATCGTCACCGGTGGCGCTGACTTTgg                                                                                                  >  2:3708161/1‑149 (MQ=255)

                                                     tcgttcATCTGCTGTACAACCGCCGATTTCACCCCAGAAGAAACCAGTTTGTCTTTGGTCTGGGAGGAGATTTTCGGGGCCGGCACTTCCCCGGACACGACCGCAATCAGGCCTTCACGCGCATCGTCACCGGTGGCGCTGACTTTGGc                                                                                                 <  2:3480676/149‑1 (MQ=255)

                                                      cgttcATCTGCTTTTCAACCGCCGATTTCACCTCAGAAGAAACCATTTTGTCTTTGGTCTGGGTGGAGAATTTCGGGTCCGGCACTTTCACGTAAACGACCGCAATCAGGCCTTCACGCGCATCGTCACCGGTGGCGCTGACTTTGGCt                                                                                                <  2:3965895/149‑1 (MQ=255)

                                                      cgttcATCTGCTGTTCAACCGCCGATTTCACCTCAGAAGAAACCAGTATGTCGTTGGTCTGGGAGGAGAATTTCGGGTCCGGCACTTTCACGGACACGACCGCAATCAGGCCTTCACGCGCATCGTCACCGGTGGCGCTGACTTTGGCt                                                                                                >  2:2791316/1‑149 (MQ=255)

                                                       gttcATCGGCTGTTCACCCGCCGTTTTCACCTCAGAAGAAACCTGTTTTTTTTTGGGGGGGGCGGATATTTTCGGGTCCGGCACTTTCCCGCAAACGACCGCAATCAGGCCTTCACGCGCATCGTCACCGGTGGCGCTGACTTTGGCtt                                                                                               <  1:1898953/149‑1 (MQ=255)

                                                             ctgctgTTCAACCGCCGATTTCACCTCAGAAGAAACCAGTTTGTCTTTGGTCTGGGAGGAGAATTTCGGGTCCGGCACTTTCACGGAAACGACCGCAATCAGGCCTTCACGCGCATCGTCACCGGTGGCGCTGACTTTGGCTTTTTTGc                                                                                         >  2:1169526/1‑149 (MQ=255)

                                                             ctgctgTTCAACCGCCGATTTCACCTCAGAAGAAACCAGTTTGTCTTTGGTCTGGGAGGAGAATTTCGGGTCCGGCACTTTCACGGAAACGACCGCAATCAGGCCTTCACGCGCATCGTCACCGGTGGCGCTGACTTTGGCTTTTTTGc                                                                                         >  1:982582/1‑149 (MQ=255)

                                                                ctgTTCAACCGCCGATTTCACCTCAGAAGAAACCAGTTTGTCTTTGGTCTGGGAGGAGAATTTCGGGTCCGGCACTTTCACGGAAACGACCGCAATCAGGCCTTCACGCGCATCGTCACCGGTGGCGCTGACTTTGGCTTTTTTGCTGt                                                                                      >  1:3630732/1‑149 (MQ=255)

                                                                ctgTTCAACCGCCGATTTCACCTCAGAAGAAACCAGTTTGTCTTTGGTCTGGGAGGAGAATTTCGGGTCCGGCACTTTCACGGAAACGACCGCAATCAGGCCTTCACGCGCATCGTCACCGGTGGCGCTGACTTTGGCTTTTTTGCTGt                                                                                      >  1:3349389/1‑149 (MQ=255)

                                                                   ttCAACCGCCTATTTCACCTAATAATAAACCTTTTTTTCTTTGGTCTGGTTGTATAATTTGGGGTCCGGAACTTTCACGGAAACGACCGCAATCAGGCCTTCACGCGCATCGTCACCGGTGGCGCTGACTTTGGCTTTTTTGCTGTAGc                                                                                   <  1:4168542/149‑1 (MQ=255)

                                                                   ttCAACCGCCGATTTCACCACAGAAGAAACCTGTTTGTCTTGGGTCTGGGCGGAGATTTTCGGGTCCGGCACTTTCACGGAAACGACCGCAATCAGGCCTTCACGCGCATCGTCACCGGTGGCGCTGACTTTGGCTTTTTTGCTGTAGc                                                                                   <  1:1662829/149‑1 (MQ=255)

                                                                   ttCAACCGCCGATTACACCACATAATAAACCAGTTTGACTTTGGTCTGGGAGGATAATTTCGGGTCCGGCACTTTCCCGCAAACGACCGCAATCAGGCCTTCACGCGCATCGTCACCGGTGGCGCTGACTTTGGCTTTTTTGCTGTAGc                                                                                   <  1:1550632/149‑1 (MQ=255)

                                                                    tCAACCGCCGATTTCACCTCAGAAGAAACCAGTTTGTCTTTGGTCTGGGAGGAGAATTTCGGGTCCGGCACTTTCACGGAAACGACCGCAATCAGGCCTTCACGCGCATCGTCACCGGTGGCGCTGACTTTGGCTTTTTTGCTGTAGcc                                                                                  >  2:1069663/1‑149 (MQ=255)

                                                                     cAACCGCCGATTTCACCTCAGAAGAAACCAGTTTGTCTTTGGTCTGGGAGGAGAATTTCGGGTCCGGCACTTTCACGGAAACGACCGCAATCAGGCCTTCACGCGCATCGTCACCGGTGGCGCTGACTTTGGCTTTTTTGCTGTAGCct                                                                                 >  2:3913825/1‑149 (MQ=255)

                                                                      aaCCGCCGATTTCACCTCAGAAGAAACCAGTTTGTCTTTGGTCTGGGAGGAGAATTTCGGGTCCGGCACTTTCACGGAAACGACCGCAATCAGGCCTTCACGCGCATCGTCACCGGTGGCGCTGACTTTGGCTTTTTTGCTGTAGCctt                                                                                >  1:4763493/1‑149 (MQ=255)

                                                                       aCCGCCGATTTCACCACATACTAAACCTGTTTGTCTTGGGTCTGGTGTGATATTTTCGGGTCGGGCACTTTCCCGCACACGACCGCAATCAGGCCTTCACGCGCATCGTCACCGGTGGCGCTGACTTTGGCTTTTTTGCTGTAGCcttc                                                                               <  1:150663/149‑1 (MQ=255)

                                                                        ccgccgATTTCACCTCCGAAGAACCCAGTGGGTCTTTGGTCTGGGAGGAGAATTTCGGGTCCGGCACTTTCACGGAAACGACCGCAATCAGGCCTTCACGCGCATCGTCACCGGTGGCGCTGACTTTGGCTTTTTTGCTGTAGCcttct                                                                              <  2:2825976/149‑1 (MQ=255)

                                                                         cgccgATTTCACCTCAGAAGAAACCAGTTTGTCTTTGGTCTGGGAGGAGAATTTCGGGTCCGGCACTTTCACGGAAACGACCGCAATCAGGCCTTCACGCGCATCGTCACCGGTGGCGCTGACTTTGGCTTTTTTGCTGTAGCcttctt                                                                             >  2:81462/1‑149 (MQ=255)

                                                                          gccgATTTCACCTCAGAAGAAACCAGTTTGTCTTTGGTCTGGGAGGAGAATTTCGGGTCCGGCACTTTCACGGAAACGACCGCAATCAGGCCTTCACGCGCATCGTCACCGGTGGCGCTGACTTTGGCTTTTTTGCTGTAGCCTTCttt                                                                            >  1:317444/1‑149 (MQ=255)

                                                                          gccgATTTCACCTCAGAAGAAACCAGTTTGTCTTTGGTCTGGGAGGAGAATTTCGGGTCCGGCACTTTCACGGAAACGACCGCAATCAGGCCTTCACGCGCATCGTCACCGGTGGCGCTGACTTTGGCTTTTTTGCTGTAGCCTTCttt                                                                            >  2:4885398/1‑149 (MQ=255)

                                                                           cctaTTACACCTAATAAGAAACCATTTTGTCTTTGGTCTGGGAGGATATTTGGGGGACCGGCACTTACACGGACACGACCGCAATCAGGCCTTCACGCGCATCGTCACCGGTGGCGCTGACTTTGGCTTTTTTGCTGTAGCCTTCTTTg                                                                           <  1:1837411/146‑1 (MQ=255)

                                                                           ccgATTTCACCTCAGAAGAAACCAGTTTGTCTTTGGTCTGGGAGGAGAATTTCGGGTCCGGCACTTTCACGGAAACGACCGCAATCAGGCCTTCACGCGCATCGTCACCGGTGGCGCTGACTTTGGCTTTTTTGCTGTAGCCTTCTTTg                                                                           >  2:3659893/1‑149 (MQ=255)

                                                                           ccgATTTCACCACAGAAGAAACCTGTTTGTCTTTGGTCTGGGAGGAGAATTTCGGGTCCGGCACTTTCACGTACACGACCGCAATCAGGCCTTCACGCGCATCGTCACCGGTGGCGCTGACTTTGGCTTTTTTGCTGTAGCCTTCTTTg                                                                           <  2:1400618/149‑1 (MQ=255)

                                                                             gATTTCACCTCAGAAGAAACCAGTTTGTCTTTGGTCTGGGAGGAGAATTTCGGGTCCGGCACTTTCACGGAAACGACCGCAATCAGGCCTTCACGCGCATCGTCACCGGTGGCGCTGACTTTGGCTTTTTTGCTGTAGCCTTCTTTGTc                                                                         >  2:574078/1‑149 (MQ=255)

                                                                              aTTTCACCTCAGAAGAAACCAGTTTGTCTTTGGTCTGGGAGGAGAATTTCGGGTCCGGCACTTTCACGGAAACGACCGCAATCAGGCCTTCACGCGCATCGTCACCGGTGGCGCTGACTTTGGCTTTTTTGCTGTAGCCTTCTTTGTcc                                                                        >  2:2283922/1‑149 (MQ=255)

                                                                               tttCACCTCAGAAGAAACCAGTTTGTCTTTGGTCTGGGAGGAGAATTTCGGGTCCGGCACTTTCACGGAAACGACCGCAATCAGGCCTTCACGCGCATCGTCACCGGTGGCGCTGACGTTGGCTTTTTTGCTGTAGCCTTCTTTGTCCa                                                                       >  2:645499/1‑149 (MQ=255)

                                                                                   aCCTCAGAAGAAACCAGTTTGTCTTTGGTCTGGGAGGAGATTTTCGGGACCGGCACTTTCACGGAAACGACCGCAATCAGGCCTTCACGCGCATCGTCACCGGTGGCGCTGACTTTGGCTTTTTTGCTGTAGCCTTCTTTGTCCATGTa                                                                   <  1:3445861/149‑1 (MQ=255)

                                                                                   aCCTCAGAAGAAACCAGTTTGTCTTTGGTCTGGGAGGAGAATTTCGGGTCCGGCACTTTCACGGAAACGACCGCAATCAGGCCTTCACGCGCATCGTCACCGGTGGCGCTGACTTTGGCTTTTTTGCTGTAGCCTTCTTTGTCCATGTa                                                                   >  2:4263548/1‑149 (MQ=255)

                                                                                   aCCTCAGAAGAAACCAGTTTGTCTTTGGTCTGGGAGGAGAATTTCGGGTCCGGCACTTTCACGGAAACGACCGCAATCAGGCCTTCACGCGCATCGTCACCGGTGGCGCTGACTTTGGCTTTTTTGCTGTAGCCTTCTTTGTCCATGTa                                                                   <  2:2441162/149‑1 (MQ=255)

                                                                                   aCCTCAGAAGAAACCAGTTTGTCTTTGGTCTGGGAGGAGAATTTCGGGTCCGGCACTTTCACGGAAACGACCGCAATCAGGCCTTCACGCGCATCGTCACCGGTGGCGCTGACTTTGGCTTTTTTGCTGTAGCCTTCTTTGTCCATGTa                                                                   >  2:1873179/1‑149 (MQ=255)

                                                                                   aCCTCAGAAGAAACCAGTTTGTCTTTGGTCTGGGAGGAGAAATTCGGGTCCGGCACTTTCACGGAAACGACCGCAATCAGGCCTTCACGCGCATCGTCACCGGTGGCGCTGACTTTGGCTTTTTTGCTGTAGCCTTCTTTGTCCATGTa                                                                   >  1:816763/1‑149 (MQ=255)

                                                                                     cTCAGAAGAAACCAGTTTGTCTTTGGTCTGGGAGGAGAATTTCGGGTCCGGCACTTTCACGGAAACGACCGCAATCAGGCCTTCACGCGCATCGTCACCGGTGGCGCTGACTTTGGCTTTTTTGCTGTAGCCTTCTTTGTCCATGTAgg                                                                 >  2:3248806/1‑149 (MQ=255)

                                                                                      tCAGAAGAAACCAGTTTGTCTTTGGTCTGGGAGGATATTTTCGGGTCCGGCACTTTCACGGAAACGACCGCAATCAGGCCTTCACGCGCATCGTCACCGGTGGCGCTGACTTTGGCTTTTTTGCTGTAGCCTTCTTTGTCCATGTAGGc                                                                <  1:2125968/149‑1 (MQ=255)

                                                                                        agaagaAACCAGTTTGTCTTTGGTCTGGGAGGAGAATTTCGGGTCCGGCACTTTCACGGAAACGACCGCAATCAGGCCTTCACGCGCATCGTCACCGGTGGCGCTGACTTTGGCTTTTTTGCTGTAGCCTTCTTTGTCCATGTAGGCGt                                                              >  1:1735022/1‑149 (MQ=255)

                                                                                         gaagaaACCTGTTTGTCTTTGGTCTGGGAGGAGAATTTCGGGTCCGGCACTTTCACGGAAACGACCGCAATCAGGCCTTCACGCGCATCGTCACCGGTGGCGCTGACTTTGGCTTTTTTGCTGTAGCCTTCTTTGTCCATGTAGGCGtt                                                             <  2:5111341/149‑1 (MQ=255)

                                                                                          aataaaCCTGTTTGTCTTTGGTCTGGGAGGATATTTTCGGGTCCGGCACTTTCCCGCACACGACCGCAATCAGGCCTTCACGCGCATCGTCACCGGTGGCGCTGACTTTGGCTTTTTTGCTGTAGCCTTCTTTGTCCATGTAGGCGTTc                                                            <  1:3246017/146‑1 (MQ=255)

                                                                                            gaaACCAGTTTGTCTTTGGTCTGGGAGGAGAATTTCGGGTCCGGCACTTTCACGGAAACGACCGCAATCAGGCCTTCACGCGCATCGTCACCGGTGGCGCTGACTTTGGCTTTTTTGCTGTAGCCTTCTTTGTCCATGTAGGCGTTCAg                                                          <  2:4437826/149‑1 (MQ=255)

                                                                                              aaCCAGTTTGTCTTTGGTCTGGGAGGAGAATTTCGGGTCCGGCACTTTCACGGAAACGACCGCAATCAGGCCTTCACGCGCATCGTCACCGGTGGCGCTGACTTTGGCTTTTTTGCTGTAGCCTTCTTTGTCCATGTAGGCGTTCAggg                                                        >  1:3810366/1‑149 (MQ=255)

                                                                                               aCCAGTTTGTCTTTGGTCTGGGTGGAGATTTTCGGGTCCGGCACTTTCACGGACACGACCGCAATCAGGCCTTCACGCGCATCGTCACCGGTGGCGCTGACTTTGGCTTTTTTGCTGTAGCCTTCTTTGTCCATGTAGGCGTTCAGGGt                                                       <  1:1640716/149‑1 (MQ=255)

                                                                                                ccAGTTTGTCTTTGGTCTGGGAGGAGAATTTCGGGTCCGGCACTTTCACGGAAACGACCGCAATCAGGCCTTCACGCGCATCGTCACCGGTGGCGCTGACTTTGGCTTTTTTGCTGTAGCCTTCTTTGTCCATGTAGGCGTTCAGGGTa                                                      >  1:4918162/1‑149 (MQ=255)

                                                                                                 cAGTTTGTCTTTGGTCTGGGAGGAGAATTTCGGGTCCGGCACTTTCACGGAAACGACCGCAATCAGGCCTTCACGCGCATCGTCACCGGTGGCGCTGACTTTGGCTTTTTTGCTGTAGCCTTCTTTGTCCATGTAGGCGTTCAGGGTAc                                                     >  2:4353265/1‑149 (MQ=255)

                                                                                                  tgTTTGTCTTTGGTGTGGGTGTATATTTGCGGGTCCGGCACTTTCCCGCACACGACCGCAATCAGGCCTTCACGCGCATCGTCACCGGTGGCGCTGACTTTGGCTTTTTTGCTGTAGCCTTCTTTGTCCATGTAGGCGTTCAGGGTACg                                                    <  1:1387837/148‑1 (MQ=255)

                                                                                                  aGTTTGTCTTTGGTCTGGGAGGAGAATTTCGGGTCCGGCACTTTCACGGAAACGACCGCAATCAGGCCTTCACGCGCATCGTCACCGGTGGCGCTGACTTTGGCTTTTTTGCTGTAGCCTTCTTTGTCCATGTAGGCGTTCAGGGTACg                                                    >  2:1582702/1‑149 (MQ=255)

                                                                                                  aGTTTGTCTTTGGTCTGGGAGGAGAATTTCGGGTCCGGCACTTTCACGGAAACGACCGCAATCAGGCCTTCACGCGCATCGTCACCGGTGGCGCTGACTTTGGCTTTTTTGCTGTAGCCTTCTTTGTCCATGTAGGCGTTCAGGGTACg                                                    >  2:1217640/1‑149 (MQ=255)

                                                                                                      tGTCTTTGGTCTGGGTGTAGAATTTCGGGTCCGGCACTTTCCCGGACACGACCGCAATCAGGCCTTCACGCGCATCGTCACCGGTGGCGCTGACTTTGGCTTTTTTGCTGTAGCCTTCTTTGTCCATGTAGGCGTTCAGGGTACGGGTc                                                <  2:2016137/149‑1 (MQ=255)

                                                                                                      tGTCTTGGGTCTGGGAGGAGAATTTCGGGTCCGGCACTTTCCCGGAAACGACCGCAATCAGGCCTTCACGCGCATCGTCACCGGTGGCGCTGACTTTGGCTTTTTTGCTGTAGCCTTCTTTGTCCATGTAGGCGTTCAGGGTACGGGTc                                                <  2:3211155/149‑1 (MQ=255)

                                                                                                       gTCTTTGGTCTGGGAGGAGAATTTCGGGTCCGGCACTTTCACGGAAACGACCGCAATCAGGCCTTCACGCGCATCGTCACCGGTGGCGCTGACTTTGGCTTTTTTGCTGTAGCCTTCTTTGTCCATGTAGGCGTTCAGGGTACGGGTCa                                               >  1:2317570/1‑149 (MQ=255)

                                                                                                       gTCTTTGGTCTGGGAGGAGAATTTCGGGTCCGGCACTTTCACGGAAACGACCGCAATCAGGCCTTCACGCGCATCGTCACCGGTGGCGCTGACTTTGGCTTTTTTGCTGTAGCCTTCTTTGTCCATGTAGGCGTTCAGGGTACGGGTCa                                               >  2:2896969/1‑149 (MQ=255)

                                                                                                          tttGGTCTGGGAGGAGAATTTCGGGTCCGGCACTTTCACGGAAACGACCGCAATCAGGCCTTCACGCGCATCGTCACCGGTGGCGCTGACTTTGGCTTTTTTGCTGTAGCCTTCTTTGTCCATGTAGGCGTTCAGGGTACGGGTCATcg                                            >  1:334169/1‑149 (MQ=255)

                                                                                                                      ggagAATTTCGGGTCCGGCACTTTCACGGAAACGACCGCAATCAGGCCTTCACGCGCATCGTCACCGGTGGCGCTGACTTTGGCTTTTTTGCTGTAGCCTTCTTTGTCCATGTAGGCGTTCAGGGTACGGGTCATCGCCGCACGGAAGc                                >  2:2114248/1‑149 (MQ=255)

                                                                                                                        agaATTTCGGGTCCGGCACTTTCACGGACACGACCGCAATCAGGCCTTCACGCGCATCGTCACCGGTGGCGCTGACTTTGGCTTTTTTGCTGTAGCCTTCTTTGTCCATGTAGGCGTTCAGGGTACGGGTCATCGCCGCACGGAAGCCt                              <  2:4788096/149‑1 (MQ=255)

                                                                                                                         gaATTTCGGGTCCGGCACTTTCACGGAAACGACCGCAATCAGGCCTTCACGCGCATCGTCACCGGTGGCGCTGACTTTGGCTTTTTTGCTGTAGCCTTCTTTGTCCATGTAGGCGTTCAGGGTACGGGTCATCGCCGCACGGAAGCCTg                             >  1:1017393/1‑149 (MQ=255)

                                                                                                                          aaTTTCGGGTCCGGCACTTTCACGGAAACGACCGCAATCAGGCCTTCACGCGCATCGTCACCGGTGGCGCTGACTTTGGCTTTTTTGCTGTAGCCTTCTTTGTCCATGTAGGCGTTCAGGGTACGGGTCATCGCCGCACGGAAGCCTGc                            >  2:3079612/1‑149 (MQ=255)

                                                                                                                           aTTACGGGTCCGGCACAATCACGGAAACGACCGCAATCACGCCTACACCAGCAACGTCACCGGAGACGCTGACAATGGCTATTTTGCTGTAGCCTACTTTGTCCATGTAGCCGTTCAGGGTACGGGTCATCGCCTCACGGACGCCAGca                           >  2:1115795/1‑148 (MQ=255)

                                                                                                                            tttCGGGTCCGGCACTTTCACGGAAACGACCGCAATCAGGCCTTCACGCGCATCGTCACCGGTGGCGCTGACTTTGGCTTTTTTGCTGTAGCCTTCTTTGTCCATGTAGGCGTTCAGGGTACGGGTCATCGCCGCACGGAAGCCTGCCa                          >  1:3542828/1‑149 (MQ=255)

                                                                                                                               cGGGTCCGCCACTTTCCCGGACACGACCGCAATCAGGCCTTCACGCGCATCGTCACCGGTGGCGCTGACTTTGGCTTTTTTGCTGTAGCCTTCTTTGTCCATGTAGGCGTTCAGGGTACGGGTCATCGCCGCACGGAAGCCTGCCAGGt                       <  1:2725159/149‑1 (MQ=255)

                                                                                                                                gggTCCGGCACTTTCACGGAAACGACCGCAATCAGGCCTTCACGCGCATCGTCACCGGTGGCGCTGACTTTGGCTTTTTTGCTGTAGCCTTCTTTGTCCATGTAGGCGTTCAGGGTACGGGTCATCGCCGCACGGAAGCCTGCCAGGTg                      >  2:2547309/1‑149 (MQ=255)

                                                                                                                                 ggTCCGGCACATTCACGGACACGACCGCAATCAGGCCTTCACGCGCATCGTCACCGGTGGCGCTGACTTTGGCTTTTTTGCTGTAGCCTTCTTTGTCCATGTAGGCGTTCAGGGTACGGGTCATCGCCGCACGGAAGCCTGCCAGGTGa                     <  2:4106936/149‑1 (MQ=255)

                                                                                                                                  gTCCGGCACTTTCACGGAAACGACCGCAATCAGGCCTTCACGCGCATCGTCACCGGTGGCGCTGACTTTGGCTTTTTTGCTGTAGCCTTCTTTGTCCATGTAGGCGTTCAGGGTACGGGTCATCGCCGCACGGAAGCCTGCCAGGTGAg                    <  1:1163985/149‑1 (MQ=255)

                                                                                                                                    ccGGCACTTTCACGGACACGACCGCAATCAGGCCTTCACGCGCATCGTCACCGGTGGCGCTGACTTTGGCTTTTTTGCTGTAGCCTTCTTTGTCCATGTAGGCGTTCAGGGTACGGGTCATCGCCGCACGGAAGCCTGCCAGGTGAGTa                  <  1:3140195/149‑1 (MQ=255)

                                                                                                                                     cGGCACTTTCACGGAAACGACCGCAATCAGGCCTTCACGCGCATCGTCACCGGTGGCGCTGACTTTGGCTTTTTTGCTGTAGCCTTCTTTGTCCATGTAGGCGTTCAGGGTACGGGTCATCGCCGCACGGAAGCCTGCCAGGTGAGTAc                 <  2:2636749/149‑1 (MQ=255)

                                                                                                                                       gCACTTTCACGGAAACGACCGCAATCAGGCCTTCACGCGCATCGTCACCGGTGGCGCTGACTTTGGCTTTTTTGCTGTAGCCTTCTTTGTCCATGTAGGCGTTCAGGGTACGGGTCATCGCCGCACGGAAGCCTGCCAGGTGAGTAccg               >  2:934144/1‑149 (MQ=255)

                                                                                                                                            ttCACGGAAACGACCGCAATCAGGCCTTCACGCGCATCGTCACCGGTGGCGCTGACTTTGGCTTTTTTGCTGTAGCCTTCTTTGTCCATGTAGGCGTTCAGGGTACGGGTCATCGCCGCACGGAAGCCTGCCAGGTGAGTACCGCCGTc          <  2:1530776/149‑1 (MQ=255)

                                                                                                                                                cGGACACGACCGCAATCAGGCCTTCACGCGCATCGTCACCGGTGGCGCTGACTTTGGCTTTTTTGCTGTAGCCTTCTTTGTCCATGTAGGCGTTCAGGGTACGGGTCATCGCCGCACGGAAGCCTGCCAGGTGAGTACCGCCGTCACGc      <  1:4564137/149‑1 (MQ=255)

                                                                                                                                                  gAAACGACCGCAATCAGGCCTTCACGCGCATCGTCACCGGTGGCGCTGACTTTGGCTTTTTTGCTGTAGCCTTCTTTGTCCATGTAGGCGTTCAGGGTACGGGTCATCGCCGCACGGAAGCCTGCCAGGTGAGTACCGCCGTCACGCTg    >  2:867516/1‑149 (MQ=255)

                                                                                                                                                    aaCGACCGCAATCAGGCCTTCACGCGCATCGTCACCGGTGGCGCTGACATTGGCTTTTTTTCTGTAGCCTTCTTTGTCCATGTAGGCGTTCATGGTACGGGTCATCGCCGCACGGACGCCTGCCAGGTGAGTACCGCCGTCACGCTGCg  >  1:243435/1‑149 (MQ=255)

CAACCACGATTTTCGCGTCGGTTGGGTTTTCCAGCAGGTATTCTGCCAGCAGTTCGTTCATCTGCTGTTCAACCGCCGATTTCACCTCAGAAGAAACCAGTTTGTCTTTGGTCTGGGAGGAGAATTTCGGGTCCGGCACTTTCACGGAAACGACCGCAATCAGGCCTTCACGCGCATCGTCACCGGTGGCGCTGACTTTGGCTTTTTTGCTGTAGCCTTCTTTGTCCATGTAGGCGTTCAGGGTACGGGTCATCGCCGCACGGAAGCCTGCCAGGTGAGTACCGCCGTCACGCTGCG  >  CP009273/3872363‑3872659

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`