Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 295,695 T→G 20.5% E317D (GAA→GAC)  paoC ← PaoABC aldehyde oxidoreductase, Moco‑containing subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273295,6950TG20.5% 706.5 / 79.7 336E317D (GAA→GAC) paoCPaoABC aldehyde oxidoreductase, Moco‑containing subunit
Reads supporting (aligned to +/- strand):  ref base T (57/203);  new base G (74/2);  total (131/205)
Fisher's exact test for biased strand distribution p-value = 2.47e-35
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 346 total aligned reads displayed.

CGTCGATCGCGATTTCGAGCGCCATCAGACCGGGGGCTTCGCCGGGCGCACGCATGGCGTTCCCTTCCGGCAAATCAAGCGTGGCGAGCCGCAGGCCGGTATGACGATTCGCCCCGGCGTAGAGTAATTCGCTTTGCTGTACCGCCGTTTCCGGCGTGCCGCCGGGCAGGTTTCCAGACCAGCTTTCATGTGAGATAGCGGTGATTTTCCCGCTCTGGTCGGCACCGATACGCAAGTGCTGAAGGGTGGCGGGGCGGTGCGTGGTGTTATTGGGAATAGAGGGGCGGGGGAGCATCA  >  CP009273/295547‑295843
                                                                                                                                                    |                                                                                                                                                    
 gtcgATCGCGATTTCGAGCGCCATCAGACCGGGGGCTTCGCCGGGCGCACGCATGGCGTTCCCTTCCGGCAAATCAAGCGTGGCGAGCCGCAGGCCGGTATGACGATTCGCCCCGGCGTAGAGTAATTCGCTTTGCTGTACCGCCGttt                                                                                                                                                     >  1:2965687/1‑149 (MQ=255)
  tcgATCGCGATTTCGAGCGCCATCAGACCGGGGGCTTCGCCGGGCGCACGCATGGCGTTCCCTTCCGGCAAATCAAGCGTGGCGAGCCGCAGGCCGGTATGACGATTCGCCCCGGCGTAGAGTAATTCGCTTTGCTGTACCGCCGTTTc                                                                                                                                                    <  1:2605809/149‑1 (MQ=255)
     aTCGCGATTTCGAGCGCCATCAGACCGGGGGCTTCGCCGGGCGCACGCATGGCGTTCCCTTCCGGCAAATCAAGCGTGGCGAGCCGCAGGCCGGTATGACGATTCGCCCCGGCGTAGAGTAATTCGCTTTGCTGTACCGCCGTGTGCgg                                                                                                                                                 >  1:3169662/1‑149 (MQ=255)
     aTCGCGATTTCGAGCGCCATCAGACCGGGGGCTTCGCCGGGCGCACGCATGGCGTTCCCTTCCGGCAAATCAAGCGTGGCGAGCCGCAGGCCGGTATGACGATTCGCCCCGGCGTAGAGTAATTCGCTTTGCTGTACCGCCGTGTGCgg                                                                                                                                                 >  1:2664396/1‑149 (MQ=255)
        gcgATTTCGAGCGCCATCAGACCGGGGGCTTCGCCGGGCGCACGCATGGCGTTCCCTTCCGGCAAATCAAGCGTGGCGAGCCGCAGGCCGGTATGACGATTCGCCCCGGCGTAGAGTAATTCGCTTTGCTGTACCGCCGTGTCCGGcct                                                                                                                                              >  1:1955398/1‑147 (MQ=255)
           aTTTCGAGCGCCATCAGACCGGGGGCTTCGCCGGGCGCACGCATGGCGTTCCCTTCCGGCAAATCAAGCGTGGCGAGCCGCAGGCCGGTATGACGATTCGCCCCGGCGTAGAGTAATTCGCTTTGCTGTACCGCCGTGTGCGGCGTggc                                                                                                                                           >  1:3638317/1‑147 (MQ=255)
            tttCGAGCGCCATCAGACCGGGGGCTTCGCCGGGCGCACGCATGGCGTTCCCTTCCGGCAAATCAAGCGTGGCGAGCCGCAGGCCGGTATGACGATTCGCCCCGGCGTAGAGTAATTCGCTTTGCTGTACCGCCGTTTACGGCGGgccg                                                                                                                                          >  2:1267601/1‑149 (MQ=255)
                 aGCGCCATCAGACCGGGGGCTTCGCCGGGCGCACGCATGGCGTTCCCTTCCGGCAAATCAAGCGTGGCGAGCCGCAGGCCGGTATGACGATTCGCCCCGGCGTAGAGTAATTCGCTTTGCTGTACCGCCGTTTACGGCGTGCCGCCggg                                                                                                                                     >  2:292113/1‑149 (MQ=255)
                  gcgcCATCAGACCGGGGGCTTCGCCGGGCGCACGCATGGCGTTCCCTTCCGGCAAATCAAGCGTGGCGAGCCGCAGGCCGGTATGACGATTCGCCCCGGCGTAGAGTAATTCGCTTTGCTGTACCGCCGTTTCCGGCGTGCCGCCGGGc                                                                                                                                    <  2:2567718/149‑1 (MQ=255)
                    gcCATCAGACCGGGGGCTTCGCCGGGCGCACGCATGGCGTTCCCTTCCGGCAAATCAAGCGTGGCGAGCCGCAGGCCGGTATGACGATTCGCCCCGGCGTAGAGTAATTCGCTTTGCTGTACCGCCGTTTGCGGCGGGCCGCCGGGccg                                                                                                                                  >  2:1289687/1‑147 (MQ=255)
                    gcCATCAGACCGGGGGCTTCGCCGGGCGCACGCATGGCGTTCCCTTCCGGCAAATCAAGCGTGGCGAGCCGCAGGCCGGTATGACGATTCGCCCCGGCGTAGAGTAATTCGCTTTGCTGTACCGCCGTTTCCGGCGTGCCGCCGGGCAg                                                                                                                                  <  2:485181/149‑1 (MQ=255)
                    gcCATCAGACCGGGGGCTTCGCCGGGCGCACGCATGGCGTTCCCTTCCGGCAAATCAAGCGTGGCGAGCCGCAGGCCGGTATGACGATTCGCCCCGGCGTAGAGTAATTCGCTTTGCTGTACCGCCGTGTCCGGCGTGCCGCCGGGccg                                                                                                                                  >  2:953801/1‑147 (MQ=255)
                    gcCATCAGACCGGGGGCTTCGCCGGGCGCACGCATGGCGTTCCCTTCCGGCAAATCAAGCGTGGCGAGCCGCAGGCCGGTATGACGATTCGCCCCGGCGTAGAGTAATTCGCTTTGCTGTACCGCCGTGTCCGGCGGGGCGCCCGGccg                                                                                                                                  >  1:4890522/1‑147 (MQ=255)
                     ccATCAGACCGGGGGCTTCGCCGGGCGCACGCATGGCGTTCCCTTTCGGCAAATCAAGCGTGGCGAGCCGCAGGCCGGTATGACGATTCGCCCCGGCGTAGAGTAATTCGCTTTGCTGTACCGCCGTTTCCGGCGTGCCGCCGGGCAgg                                                                                                                                 <  1:1016003/149‑1 (MQ=255)
                       aTCAGACCGGGGGCTTCGCCGGGCGCACGCATGGCGTTCCCTTCCGGCAAATCAAGCGTGGCGAGCCGCAGGCCGGTATGACGATTCGCCCCGGCGTAGAGTAATTCGCTTTGCTGTACCGCCGTTTCCGGCGTGCCGCCGGGCCGGtt                                                                                                                               >  2:5146512/1‑149 (MQ=255)
                       aTCAGACCGGGGGCTTCGCCGGGCGCACGCATGGCGTTCCCTTCCGGCAAATCAAGCGTGGCGAGCCGCAGGCCGGTATGACGATTCGCCCCGGCGTAGAGTAATTCGCTTTGCTGTACCGCCGTTTCCGGCGTGCCGCCGGGCAGGtt                                                                                                                               <  1:4575582/149‑1 (MQ=255)
                        tCAGACCGGGGGCTTCGCCGGGCGCACGCATGGCGTTCCCTTCCGGCAAATCAAGCGTGGCGAGCCGCAGGCCGGTATGACGATTCGCCCCGGCGTAGAGTAATTCGCTTTGCTGTACCGCCGTGTGCGGCGGGCCGCCGGGGCGGttt                                                                                                                              >  1:1405871/1‑149 (MQ=255)
                         cAGACCGGGGGCTTCGCCGGGCGCACGCATGGCGTTCCCTTCCGGCAAATCAAGCGTGGCGAGCCGCAGGCCGGTATGACGATTCGCCCCGGCGTAGAGTAATTCGCTTTGCTGTACCGCCGTTTCCGGCGTGCCGCCGGGCAGGTTTc                                                                                                                             <  1:4924724/149‑1 (MQ=255)
                          aGACCGGGGGCTTCGCCGGGCGCACGCATGGCGTTCCCTTCCGGCAAATCAAGCGTGGCGAGCCGCAGGCCGGTATGACGATTCGCCCCGGCGTAGAGTAATTCGCTTTGCTGTACCGCCGTTTGCGGCGGGCCCCCCGGCAGGgttgc                                                                                                                            >  1:1153044/1‑147 (MQ=255)
                          aGACCGGGGGCTTCGCCGGGCGCACGCATGGCGTTCCCTTCCGGCAAATCAAGCGTGGCGAGCCGCAGGCCGGTATGACGATTCGCCCCGGCGTAGAGTAATTCGCTTTGCTGTACCGCCGTTTCCGGCGTGCCGCCGGGCAGGTTTcc                                                                                                                            <  1:675997/149‑1 (MQ=255)
                          aGACCGGGGGCTTCGCCGGGCGCACGCATGGCGTTCCCTTCCGGCAAATCAAGCGTGGCGAGCCGCAGGCCGGTATGACGATTCGCCCCGGCGTAGAGTAATTCGCTTTGCTGTACCGCCGTTTCCGGCGTGCCGCCGGGCAGGTTTcc                                                                                                                            <  2:3314978/149‑1 (MQ=255)
                             ccGGGGGCTTCGCCGGGCGCACGCATGGCGTTCCCTTCCGGCAAATCAAGCGTGGCGAGCCGCAGGCCGGTATGACGATTCGCCCCGGCGTAGAGTAATTCGCTTTGCTGTACCGCCGTTTCCGGCGTGCCGCCGGGCAGGTTTCCAGa                                                                                                                         <  1:2997022/149‑1 (MQ=255)
                               gggggCTTCGCCGGGCGCACGCATGGCGTTCCCTTCCGGCAAATCAAGCGTGGCGAGCCGCAGGCCGGTATGACGATTCGCCCCGGCGTAGAGTAATTCGCTTTGCTGTACCGCCGTGTCCCGCGTGCCCCCGGGCAGGGGTCCACAcc                                                                                                                       >  2:742563/1‑149 (MQ=255)
                                ggggCTTCGCCGGGCGCACGCATGGCGTTCCCTTCCGGCAAATCACGCGTGGCGAGCCGCAGGCCGGTCTGACGATTCGCCCCGGCGTAGAGTAAGTCGCTTTGCTGTACCGCCGTGTACCGCGGGCCCCCGGGCCGGCTGCCACACCa                                                                                                                      >  1:5147891/1‑149 (MQ=255)
                                ggggCTTCGCCGGGCGCACGCATGGCGTTCCCTTCCGGCAAATCAAGCGTGGCGAGCCGCAGGCCGGTATGACGATTCGCCCCGGCGTAGAGTAATTCGCTTTGCTGTACCGCCGTTTCCGGCGTGCCGCCGGGCAGGTTTCCAGACCa                                                                                                                      <  1:705413/149‑1 (MQ=255)
                                 gggCTTCGCCGGGCGCACGCATGGCGTTCCCTTCCGGCAAATCAAGCGTGGCGAGCCGCAGGCCGGTATGACGATTCGCCCCGGCGTAGAGTAATTCGCTTTGCTGTACCGCCGTTTCCGGCGTGCCGCCGGGCAGGTTTCCAGACCAg                                                                                                                     <  1:819508/149‑1 (MQ=255)
                                  ggCTTCGCCGGGCGCACGCATGGCGTTCCCTTCCGGCAAATCAAGCGTGGCGAGCCGCAGGCCGGTATGACGATTCGCCCCGGCGTAGAGTAATTCGCTTTGCTGTACCGCCGTTTCCGGCGTGCCGCCGGGCAGGTTTCCAGACCAGc                                                                                                                    <  1:2805449/149‑1 (MQ=255)
                                   gCTTCGCCGGGCGCACGCATGGCGTTCCCTTCCGGCAAATCAAGCGTGGCGAGCCGCAGGCCGGTATGACGATTCGCCCCGGCGTAGAGTAATTCGCTTTGCTGTACCGCCGTTTCCGGCGTGCCGCCGGGCAGGTTTCCAGACCAGCt                                                                                                                   <  1:4048548/149‑1 (MQ=255)
                                   gCTTCGCCGGGCGCACGCATGGCGTTCCCTTCCGGCAAATCAAGCGTGGCGAGCCGCAGGCCGGTATGACGATTCGCCCCGGCGTAGAGTAATTCGCTTTGCTGTACCGCCGTGTACGGCGTGCCGCCGGGCACGTTTCCATACCAGcg                                                                                                                   >  2:5017044/1‑148 (MQ=255)
                                      tCGCCGGGCGCACGCATGGCGTTCCCTTCCGGCAAATCAAGCGTGGCGAGCCGCAGGCCGGTATGACGATTCGCCCCGGCGTAGAGTAATTCGCTTTGCTGTACCGCCGTTTCCGGCGTGCCGCCGGGCAGGTTTCCAGACCAGCTTTc                                                                                                                <  1:4992994/149‑1 (MQ=255)
                                        gCCGGGCGCACGCATGGCGTTCCCTTCCGGCAAATCAAGCGTGGCGAGCCGCAGGCCGGTATGACGATTCGCCCCGGCGTAGAGTAATTCGCTTTGCTGTACCGCCGTTTCCGGCGTGCCGCCGGGCAGGTTTCCAGACCAGCTTTCAt                                                                                                              <  2:3624857/149‑1 (MQ=255)
                                             gcgcACGCATGGCGTTCCCTTCCGGCAAATCAAGCGTGGCGAGCCGCAGGCCGGTATGACGATTCGCCCCGGCGTAGAGTAATTCGCTTTGCTGTACCGCCGTTTACGGCGTGCCGCCGGGCAGGTTTACAGACCAGCTTTCAAGTgat                                                                                                         >  1:3850087/1‑148 (MQ=255)
                                               gcacgcaTGGCGTTCCCTTCCGGCAAATCAAGCGTGGCGAGCCGCAGGCCGGTATGACGATTCGCCCCGGCGTAGAGTAATTCGCTTTGCTGTACCGCCGTTTCCGGCGTGCCGCCGGGCAGGTTTCCAGACCAGCTTTCATGTGAGAt                                                                                                       <  2:1334158/149‑1 (MQ=255)
                                               gcacgcaTGGCGTTCCCTTCCGGCAAATCAAGCGTGGCGAGCCGCAGGCCGGTATGACGATTCGCCCCGGCGTAGAGTAATTCGCTTTGCTGTACCGCCGTTTCCGGCGTGCCGCCGGGCAGGTTTCCAGACCAGCTTTCATGTGAGAt                                                                                                       <  2:3951512/149‑1 (MQ=255)
                                               gcacgcaTGGCGTTCCCTTCCGGCAAATCAAGCGTGGCGAGCCGCAGGCCGGTATGACGATTCGCCCCGGCGTAGAGTAATTCGCTTTGCTGTACCGCCGTTTCCGGCGTGCCGCCGGGCAGGTTTCCAGACCAGCTTTCATGTGAGAt                                                                                                       <  1:4356618/149‑1 (MQ=255)
                                                 acgcaTGGCGGTCCCTTCCGGCAAATCAAGCGTGGCGAGCCGCAGGCCGGTATGACGATTCGCCCCGGCGTAGAGTAATTCGCTTTGCTGTACCGCCGTTTCCGGCGTGCCGCCGGGCAGGTTTCCAGACCAGCTTTCATGTGAGATAg                                                                                                     <  2:2718357/149‑1 (MQ=255)
                                                   gcaTGGCGTTCCCTTCCGGCAAATCAAGCGTGGCGAGCCGCAGGCCGGTATGACGATTCGCCCCGGCGTAGAGTAATTCGCTTTGCTGTACCGCCGTGTCCGGCGGGCCGCCGGGCCGGGTTCCACACCACCAGTCATGTGAGATAGCg                                                                                                   >  1:2242399/1‑149 (MQ=255)
                                                    cttGGCGTTCCCTTCCTGCAAATCAAGCGTGGCGAGCCGCAGGCCGGTATGACGATTCGCCCCGGCGTTGAGTAATTCGCTTTGCTGTACCGCCGTTTGCGGCGTGCCGCCGGGCAGGTTTCCAGTCCAGCTTTCATGTGAGATAGCgg                                                                                                  <  2:2270105/147‑1 (MQ=255)
                                                     aTGGCGTTCCCTTCCGGCAAATCAAGCGTGGCGAGCCGCAGGCCGGTATGACGATTCGCCCCGGCGTAGAGTAATTCGCTTTGCTGTACCGCCGTTTCCGGCGTGCCGCCGGGCAGGTTTCCAGACCAGCTTTCATGTGAGATAGCGGt                                                                                                 <  1:1571594/149‑1 (MQ=255)
                                                     aTGGCGTTCCCTTCCGGCAAATCAAGCGTGGCGAGCCGCAGGCCGGTATGACGATTCGCCCCGGCGTAGAGTAATTCGCTTTGCTGTACCGCCGTTTCCGGCGTGCCGCCGGGCAGGTTTCCAGACCAGCTTTCATGTGAGATAGCGGt                                                                                                 <  2:4593910/149‑1 (MQ=255)
                                                      tGGCGTTCCCTTCCGGCAAATCAAGCGTGGCGAGCCGCAGGCCGGTATGACGATTCGCCCCGGCGTAGAGTAATTCGCTTTGCTGTACCGCCGTTTCCGGCGTGCCGCCGGGCAGGTTTCCAGACCAGCTTTCATGTGAGATAGCGGTg                                                                                                <  2:2520289/149‑1 (MQ=255)
                                                      tGGCGTTCCCTTCCGGCAAATCAAGCGTGGCGAGCCGCAGGCCGGTATGACGATTCGCCCCGGCGTAGAGTAATTCGCTTTGCTGTACCGCCGTTTCCGGCGTGCCGCCGGGCAGGTTTCCAGACCAGCTTTCATGTGAGATAGCGGTg                                                                                                <  2:648676/149‑1 (MQ=255)
                                                             cccTTCCGGCAAATCAAGCGTGGCGAGCCGCAGGCCGGTATGACGATTCGCCCCGGCGTAGAGTAATTCGCTTTGCTGTACCGCCGTTTCCGGCGTGCCGCCGGGCAGGTTTCCAGACCAGCTTTCATGTGAGATAGCGGTGAttttac                                                                                         >  2:2823659/1‑147 (MQ=255)
                                                             cccTTCCGGCAAATCAAGCGTGGCGAGCCGCAGGCCGGTATGACGATTCGCCCCGGCGTAGAGTAATTCGCTTTGCTGTACCGCCGTTTCCGGCGTGCCGCCGGGCAGGTTTCCAGACCAGCGTTCATGTGAGATAGCGGTGAttttac                                                                                         >  2:1154182/1‑147 (MQ=255)
                                                             cccTTCCGGCAAATCAAGCGTGGCGAGCCGCAGGCCGGTATGACGATTCGCCCCGGCGTAGAGTAATTCGCTTTGCTGTACCGCCGTGTGCGGCGGGCCGCCCGGCCGGTTTGCCCACCAGCGTTCATGTGGGATAGCGGGGGttttaa                                                                                         >  2:4346592/1‑147 (MQ=255)
                                                             cccTTCCGGCAAATCAAGCGTGGCGAGCCGCAGGCCGGTATGACGATTCGCCCCGGCGTAGAGTAATTCGCTTTGCTGTACCGCCGTGTCCGGCGTGCCGCCGGGCAGGGTTCCAGACCAGCTTTCATGGGAGATAGCGGTGATTTTcc                                                                                         >  2:1966403/1‑149 (MQ=255)
                                                                  ccGGCAAATCAAGCGTGGCGAGCCGCAGGCCGGTATGACGATTCGCCCCGGCGTAGAGTAATTCGCTTTGCTGTACCGCCGTGTGCCGCGGGCCGCCGGGCCGGTTTCCAGACCCGCGTTCATGTGAGATCGCGGTGATTTTACCGcgc                                                                                    >  2:1482469/1‑147 (MQ=255)
                                                                    ggCAAATCAAGCGTGGCGAGCCGCAGGCCGGTATGACGATTCGCCCCGGCGTAGAGTAATTCGCTTTGCTGTACCGCCGTTTCCGGCGTGCCGCCGGGCAGGTTTCCAGACCAGCTTTCATGTGAGATAGCGGTGATTTTCCCGCTCTg                                                                                  <  2:2467357/149‑1 (MQ=255)
                                                                    ggCAAATCAAGCGTGGCGAGCCGCAGGCCGGTATGACGATTCGCCCCGGCGTAGAGTAATTCGCTTTGCTGTACCGCCGTTTACGGCGTGCCGCCGGGCAGGTTTCCAGACCACCGTTAATGTTAGATAGCGGTGATTTTACCGCTCTg                                                                                  >  2:1095623/1‑149 (MQ=255)
                                                                     gCAAATCAAGCGTGGCGAGCCGCAGGCCGGTATGACGATTCGCCCCGGCGTAGAGTAATTCGCTTTGCTGTACCGCCGTTTGCGGCGTGGCGCCGGGCCGGTTTCCAGACCACCGTTCATGTGGGATAGCGGTGTTTTTACCGCTCggg                                                                                 >  2:3937184/1‑149 (MQ=255)
                                                                      cAAATCAAGCGTGGCGAGCCGCAGGCCGGTATGACGATTCGCCCCGGCGTAGAGTAATTCGCTTTGCTGTACCGCCGTTTCCGGCGTGCCGCCGGGCAGGTTTCCAGACCAGCTTTCATGTGAGATAGCGGTGATTTTCCCGCTCTGGt                                                                                <  2:1580568/149‑1 (MQ=255)
                                                                      cAAATCAAGCGTGGCGAGCCGCAGGCCGGTATGACGATTCGCCCCGGCGTAGAGTAATTCGCTTTGCTGTACCGCCGTTTCCGGCGTGCCGCCGGGCAGGTTTCCAGACCAGCTTTCATGTGAGATAGCGGTGATTTTCCCGCTCTGGt                                                                                <  2:1835257/149‑1 (MQ=255)
                                                                          tCAAGCGTGGCGAGCCGCAGGCCGGTATGACGATTCGCCCCGGCGTAGAGTAATTCGCTTTGCTGTACCGCCGTTTCCGGCGTGCCGCCGGGCAGGTTTCCAGACCAGCTTTCATGTGAGATAGCGGTGATTTTCCCGCTCTGGTCGGc                                                                            <  1:5152644/149‑1 (MQ=255)
                                                                            aaGCGTGGCGAGCCGCAGGCCGGTATGACGATTCGCCCCGGCGTAGAGTAATTCGCTTTGCTGTACCGCCGTTTCCGGCGTGCCGCCGGGCAGGTTTCCAGACCAGCTTTCATGTGAGATAGCGGTGATTTTCCCGCTCTGGTCGGCAc                                                                          <  2:4229986/149‑1 (MQ=255)
                                                                            aaGCGTGGCGAGCCGCAGGCCGGTATGACGATTCGCCCCGGCGTAGAGTAATTCGCTTTGCTGTACCGCCGTTTCCGGCGTGCCGCCGGGCAGGTTTCCAGACCAGCTTTCATGTGAGATAGCGGGGATTTTCCCGCTCTGggcggccc                                                                          >  2:4492938/1‑147 (MQ=255)
                                                                                      aGCCGCAGGCCGGTATGACGATTCGCCCCGGCGTAGAGTAATTCGCTTTGCTGTACCGCCGTTTGCGGCGGTCCGCCGGGCAGGGTTGGAGAACACCTATCAAGTGGGATATCGGGGTTTTTAACGCGCGGGTGGGCCCCCCTACGcca                                                                >  1:4053596/1‑147 (MQ=255)
                                                                                      aGCCGCAGGCCGGTATGACGATTCGCCCCGGCGTAGAGTAATTCGCTTTGCTGTACCGCCGTGTGAGGCGTGCCGCCCGGCAGGTTTACACACAACCAATAATTTGATATATCGGTGATTTTGACCCACAGGTAGGCACCCATaaacaa                                                                >  1:245977/1‑144 (MQ=255)
                                                                                       gCCGCAGGCCGGTATGACGATTCGCCCCGGCGTAGAGTAATTCGCTTTGCTGTACCGCCGTTTCCGGCGTGCCGCCGGGCAGGTTTCCAGACCAGCTTTCATGTGAGATAGCGGTGATTTTCCCGCTCTGGTCGGCACCGATACGCAAg                                                               <  1:3905395/149‑1 (MQ=255)
                                                                                       gCCGCAGGCCGGTATGACGATTCGCCCCGGCGTAGAGTAATTCGCTTTGCTGTACCGCCGTGTGCGGCGGGCCGCCCGGCCCGTTTGCAGAACACCATTCAAGTTTGATATCGGTGATTTTACCCCACAGGTCGGCACCCAAACGccaa                                                               >  1:5038011/1‑146 (MQ=255)
                                                                                          gCAGGCCGGTATGACGATTCGCCCCGGCGTAGAGTAATTCGCTTTGCTGTACCGCCGTTTACGGCGGTCCGCCCGGCACGTTTACAGAACATCTTTAAATTGATTTATGGGTTATTTTAAAGCGATGGTTGGCAACCATACGCaatttg                                                            >  1:2526482/1‑145 (MQ=255)
                                                                                            aGGCCGGTATGACGATTCGCCCCGGCGTAGAGTAATTCGCTTTGCTGTACCGCCGTTTCCGGCGTGCCGCCGGGCAGGTTTCCAGACCAGCTTTCATGTGAGATAGCGGTGATTTTCCCGCTCTGGTCGGCACCGATACGCAAGTGCTg                                                          <  2:1928288/149‑1 (MQ=255)
                                                                                            aGGCCGGTATGACGATTCGCCCCGGCGTAGAGTAATTCGCTTTGCTGTACCGCCGTTTCCGGCGTGCCGCCGGGCAGGTTTCCAGACCAGCTTTCATGTGAGATAGCGGTGATTTTCCCGCTCTGGTCGGCACCGATACGCAAGTGCTg                                                          <  1:3372158/149‑1 (MQ=255)
                                                                                            aGGCCGGTATGACGATTCGCCCCGGCGTAGAGTAATTCGCTTTGCTGTACCGCCGTGTCCGGCGTGCCGCCGGGCCGGTTTCCAGACCAGCTTTCAAGTGGGATAGCGGTGATTTTCCCGCTCTGGTCGGCACCGATACGCAActgctg                                                          >  2:91142/1‑149 (MQ=255)
                                                                                              gCCGGTATGACGATTCGCCCCGGCGTAGAGTAATTCGCTTTGCTGTACCGCCGTTTCCGGCGTGCCGCCGGGCAGGTTTCCAGACCAGCTTTCATGTGAGATAGCGGTGATTTTCCCGCTCTGGTCGGCACCGATACGCAAGTGCTGaa                                                        <  1:2108411/149‑1 (MQ=255)
                                                                                              gCCGGTATGACGATTCGCCCCGGCGTAGAGTAATTCGCTTTGCTGTACCGCCGTTTCCGGCGTGCCGCCGGGCAGGTTTCCAGACCAGCTTTCATGTGAGATAGCGGTGATTTTCCCGCTCTGGTCGGCACCGATACGCAAGTGCTGaa                                                        <  1:4714892/149‑1 (MQ=255)
                                                                                                 ggTATGACGATTCGCCCCGGCGTAGAGTAATTCGCTTTGCTGTACCGCCGTTTCCGGCGTGCCGCCGGGCAGGTTTCCAGACCAGCTTTCATGTGAGATAGCGGTGATTTTCCCGCTCTGGTCGGCACCGATACGCAAGTGCTGAAggg                                                     <  1:1731773/149‑1 (MQ=255)
                                                                                                    aTGACGATTCGCCCCGGCGTAGAGTAATTCGCTTTGCTGTACCGCCGTTTCCGGCGGGCCGCCGGGCAGGTTTACCGACCACCTTTAATGTGAGATAGCGGTGATTTTCACGCTCTGGTCGGCACCGATACGCAAGTGCTGAAGGGTgg                                                  >  2:2440263/1‑149 (MQ=255)
                                                                                                    aTGACGATTCGCCCCGGCGGAGAGTAATTCGCTTTGCTGTACCGCCGTTTCCGGCGTGCCGCCGGGCAGGTTTCCAGACCAGCTTTCATGTGAGATAGCGGTGATTTTCCCGCTCTGGTCGGCACCGATACGCAAGTGCTGAAGGGTgg                                                  <  2:3031509/149‑1 (MQ=255)
                                                                                                       aCGATTCGCCCCGGCGTAGAGTAATTCGCTTTGCTGTACCGCCGTTTCCGGCGTGCCGCCGGGCAGGTTTCCAGACCAGCTTTCATGTGAGATAGCGGTGATTTTCCCGCTCTGGTCGGCACCGATACGCAAGTGCTGAAGGGTggcgg                                               <  2:1061938/149‑1 (MQ=255)
                                                                                                       aCGATTCGCCCCGGCGTAGAGTAATTCGCTTTGCTGTACCGCCGTTTCCGGCGTGCCGCCCGGCAGGTTTCCAGAACACCTTTCATGTGAGATATCGGTGATTTTACCCCACTGGGCGGCACCCATACGCAAGTGCTGAAGGGTggcgg                                               >  2:3403596/1‑149 (MQ=255)
                                                                                                            tCGCCCCGGCGTAGAGTAATTCGCTTTGCTGTACCGCCGTTTCCGGCGTGCCGCCGGGCAGGTTTCCAGACCAGCTTTCATGTGAGATAGCGGTGATTTTCCCGCTCTGGTCGGCACCGATACGCAAGTGCTGAAGGGTggcggggcgg                                          <  2:939207/149‑1 (MQ=255)
                                                                                                            tCGCCCCGGCGTAGAGTAATTCGCTTTGCTGTACCGCCGTTTCCGGCGTGCCGCCGGGCAGGTTTCCAGACCAGCTTTCATGTGAGATAGCGGTGATTTTCCCGCTCTGGTCGGCACCGATACACAAGTGCTGAAGGGTggcggggcgg                                          <  1:1441889/149‑1 (MQ=255)
                                                                                                             cGCCCCGGCGTAGAGTAATTCGCTTTGCTGTACCGCCGTTTCCGGCGTGCCGCCGGGCAGGTTTCCAGACCAGCTTTCATGTGAGATAGCGGTGATTTTCCCGCTCTGGTCGGCACCGATACGCAAGTGCTGAAGGGTGGCGGGGCGGt                                         <  1:4184771/149‑1 (MQ=255)
                                                                                                             cGCCCCGGCGTAGAGTAATTCGCTTTGCTGTACCGCCGTTTCCGGCGTGCCGCCGGGCAGGTTTCCAGACCAGCTTTCATGTGAGATAGCGGTGATTTTCCCGCTCTGGTCGGCACCGATACGCAAGTGCTGAAGGGTGGCGGGGCGGt                                         <  1:287795/149‑1 (MQ=255)
                                                                                                              gCCCCGGCGTAGAGTAATTCGCTTTGCTGTACCGCCGTTTCCGGCGTGCCGCCGGGCAGGTTTCCAGACCAGCTTTCATGTGAGATAGCGGTGATTTTCCCGCTCTGGTCGGCACCGATACGCAAGTGCTGAAGGGTGGCGGGGCGGTg                                        <  2:4538945/149‑1 (MQ=255)
                                                                                                              gCCCCGGCGTAGAGTAATTCGCTTTGCTGTACCGCCGTTTCCGGCGTGCCGCCGGGCAGGTTTCCAGACCAGCTTTCATGTGAGATAGCGGTGATTTTCCCGCTCTGGTCGGCACCGATACGCAAGTGCTGAAGGGTGGCGGGGCGGTg                                        <  2:3261264/149‑1 (MQ=255)
                                                                                                                 ccGGCGTAGAGTAAGTCGCTTTGCTGTACCGCCGTTTCCGGCGTGCCGCCGGGCAGGTTTCCAGACCAGCTTTCATGTGAGATAGCGGTGATTTTCCCGCTCTGGTCGGCACCGATACGCGAGTGCTGAAGGGTGGCGGGGCGGTGCgt                                     <  2:2219841/149‑1 (MQ=255)
                                                                                                                    gCGTAGAGTAATTCGCTTTGCTGTACCGCCGTTTCCGGCGTGCCGCCGGGCAGGTTTCCAGACCAGCTTTCATGTGAGATAGCGGTGATTTTCCCGCTCTGGTCGGCACCGATACGCAAGTGCTGAAGGGTGGCGGGGCGGTGCgtggt                                  <  2:5005969/149‑1 (MQ=255)
                                                                                                                    gCGTAGAGTAATTCGCTTTGCTGTACCGCCGTGTACGGCGTGCCGCCGGGCAGGTTTACATAACATCGTTCATGTGAGTTATAGGTGATTTTACCGCTCTGGTCGGCAACGATACGCAAATGCTTAAGGGTTGCGGGGGGGGGCggggg                                  >  2:560521/1‑148 (MQ=255)
                                                                                                                     cGTAGAGTAATTCGCTTTGCTGTACCGCCGTTTCCGGCGTGCCGCCGGGCAGGTTTCCAGACCAGCTTTCATGTGAGATAGCGGTGATTTTCCCGCTCTGGTCGGCACCGATACGCAAGTGCTGAAGGGTGGCGGGGCGGTGCgtggtg                                 <  2:4054839/149‑1 (MQ=255)
                                                                                                                     cGTAGAGTAATTCGCTTTGCTGTACCGCCGGTTCCGGCGTGCCGCCGGGCAGGTTTCCAGACCAGCTTTCATGTGAGATAGCGGTGATTTTCCCGCTCTGGTCGGCACCGATACGCAAGTGCTGAAGGGTGGCGGGGCGGTGCgtggtg                                 <  2:1811348/149‑1 (MQ=255)
                                                                                                                      gTAGAGTAATTCGCTTTGCTGTACCGCCGTTTCCGGCGTGCCGCCGGGCAGGTTTACACACCAGCTTTCATGTGAGATAGCGGTGATTTTCCCGCGCTGGTCGGCACCGATACGCAAGTGCTGAAGGGTGGCGGGGCGGGGCGGGgtgt                                >  2:4732236/1‑149 (MQ=255)
                                                                                                                       tAGAGTAATTCGCTTTGCTGTACCGCCGTTTCCGGCGTGCCGCCGGGCAGGTTTCCAGACCAGCTTTCATGTGAGATAGCGGTGATTTTCCCGCTCTGGTCGGCACCGATACGCAAGTGCTGAAGGGTGGCGGGGCGGTGCGTGGTGtt                               <  1:3239069/149‑1 (MQ=255)
                                                                                                                        agagTAATTCGCTTTGCTGTACCGCCGTTTGCGGCGTGCCGCCGGGCCGGTTTGCAGAACAGCTTTCATGTGATATAGCGGTGATTTTACCGCTCTGGTCGGCACCGAAACGCAAGTGCTGAAGGGTGGCGGGGCGGTGCGTGGTGTTa                              >  2:369677/1‑149 (MQ=255)
                                                                                                                        agagTAATTCGCTTTGCTGTACCGCCGTTTCCGGCGTGCCGCCGGGCAGGTTTCCAGACCAGCTTTCATGTGAGATAGCGGTGATTTTCCCGCTCTGGTCGGCACCGATACGCAAGTGCTGAAGGGTGGCGGGGCGGTGCGTGGTGTTa                              <  1:3780654/149‑1 (MQ=255)
                                                                                                                              aTTCGCTTTGCTGTACCGCCGTTTGCGGCGTGCCGCCGGGCAGGTTTCCAGACCAGCTTTCATGTGAGATAGCGGTGTTTTTCCCGCTCTGGTCGGCACCGATACGCAAGTGCTGGAGGGTGGCGGGGCGGTGCGTGGGGGTATTgggg                        >  2:2018739/1‑148 (MQ=255)
                                                                                                                               ttCGCTTTGCTGTACCGCCGTTTCCGGCGTGCCGCCGGGCAGGTTTCCAGACCAGCTTTCATGTGAGATAGCGGTGATTTTCCCGCTCTGGTCGGCACCGATACGCAAGTGCTGAAGGGTGGCGGGGCGGTGCGTGGTGTTATTGGGaa                       <  2:1152708/149‑1 (MQ=255)
                                                                                                                               ttCGCTTTGCTGTACCGCCGTTTCCGGCGTGCCGCCGGGCAGGTTTCCAGACCAGCTTTCATGTGAGATAGCGGTGATTTTCCCGCTCTGGTCGGCACCGATACGCAAGTGCTGAAGGGTGGCGGGGCGGTGCGTGGTGTTATTGGGaa                       <  1:3308039/149‑1 (MQ=255)
                                                                                                                                 cGCTTTGCTGTACCGCCGTTTCCGGCGTGCCGCCGGGCAGGTTTCCAGACCAGCTTTCATGTGAGATAGCGGTGATTTTCCCGCTCTGGTCGGCACCGATACGCAAGTGCTGAAGGGTGGCGGGGCGGTGCGTGGTGTTATTGGGAATa                     <  1:1833749/149‑1 (MQ=255)
                                                                                                                                 cGCTTTGCTGTACCGCCGTTTCCGGCGTGCCGCCGGGCAGGTTTCCAGACCAGCTTTCATGTGAGATAGCGGTGATTTTCCCGCTCTGGTCGGCACCGATACGCAAGTGCTGAAGGGTGGCGGGGCGGTGCGTGGTGTTATTGGGAATa                     <  1:4445389/149‑1 (MQ=255)
                                                                                                                                    tttGCTGTACCGCCGTTTCCGGCGTGCCGCCGGGCAGGTTTCCAGACCAGCTTTCATGTGAGATAGCGGTGATTTTCCCGCTCTGGTCGGCACCGATACGCAAGTGCTGAAGGGTGGCGGGGCGGTGCGTGGTGTTATTGGGAATagag                  <  2:3885016/149‑1 (MQ=255)
                                                                                                                                    tttGCTGTACCGCCGTTTCCCGCGTGCCGCCGGGCAGGTTTACACAACAGCTTTCATGTGATATATCGGTGATTTTCACGCTCTGGTCGGCACCGATAACCAAGTTCTGAAGGGTGGCGGGGCGGTGCGTGGTGTTATTGGGGATagag                  >  2:2358211/1‑149 (MQ=255)
                                                                                                                                     ttGCTGTACCGCCGTTTCCGGCGTGCCGCCGGGCAGGTTTCCAGACCAGCTTTCATGTGAGATAGCGGTGATTTTCCCGCTCTGGTCGGCACCGATACGCAAGTGCTGAAGGGTGGCGGGGCGGTGCGTGGTGTTATTGGGAATAGAgg                 <  2:2319032/149‑1 (MQ=255)
                                                                                                                                      tGCTGTACCGCCGTTTCCGGCGTGCCGCCGGGCAGGTTTCCAGACCAGCTTTCATGTGAGATAGCGGTGATTTTCCCGCTCTGGTCGGCACCGATACGCAAGTGCTGAAGGGTGGCGGGGCGGTGCGTGGTGTTATTGGGAATAGAggg                <  1:1147302/149‑1 (MQ=255)
                                                                                                                                       gCTGTACCGCCGTTTCCGGCGTGCCGCCGGGCAGGTTTCCAGACCAGCTTTCATGTGAGATAGCGGTGATTTTCCCGCTCTGGTCGGCACCGATACGCAAGTGCTGAAGGGTGGCGGGGCGGTGCGTGGTGTTATTGGGAATAGAgggg               <  2:4479953/149‑1 (MQ=255)
                                                                                                                                       gCTGTACCGCCGTTTCCGGCGTGCCGCCGGGCAGGTTTCCAGACCAGCTTTCATGTGAGATAGCGGTGATTTTCCCGCTCTGGTCGGCACCGATACGCAAGTGCTGAAGGGTGGCGGGGCGGTGCGTGGTGTTATTGGGAATAGAgggg               <  1:4980454/149‑1 (MQ=255)
                                                                                                                                         tGTACCGCCGTTTCCGGCGTGCCGCCGGGCAGGTTTCCAGACCAGCTTTCATGTGAGATAGCGGTGATTTTCCCGCTCTGGTCGGCACCGATACGCAAGTGCTGAAGGGTGGCGGGGCGGTGCGTGGTGTTATTGGGAATAGAGGGGCg             <  2:3169662/149‑1 (MQ=255)
                                                                                                                                               gccgTTTCCGGCGTGCCGCCGGGCAGGTTTCCAGACCAGCTTTCATGTGAGATAGCGGTGATTTTACCGCTCTGGTCGGCACCGATACGCAAGTGCTGAAGGGTGGCGGGGCGGTGCGTGGTGTTATTGGGAATAGAGGGGCGGGGGAg       >  2:2741433/1‑149 (MQ=255)
                                                                                                                                                 cgTTTCCGGCGTGCCGCCGGGCAGGTTTCCAGACCAGCTTTCATGTGAGATAGCGGTGATTTTCCCGCTCTGGTCGGCACCGATACGCAAGTGCTGAAGGGTGGCGGGGCGGTGCGTGGTGTTATTGGGAATAGAGGGGCGGGGGAGCa     <  1:3724517/149‑1 (MQ=255)
                                                                                                                                                  gTTTCCGGCGTGCCGCCGGGCAGGTTTCCAGACCAGCTTTCATGTGAGATAGCGGTGATTTTCCCGCTCTGGTCGGCACCGATACGCAAGTGCTGAAGGGTGGCGGGGCGGTGCGTGGTGTTATTGGGAATAGAGGGGCGGGGGAGCAt    >  1:1800139/1‑149 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
CGTCGATCGCGATTTCGAGCGCCATCAGACCGGGGGCTTCGCCGGGCGCACGCATGGCGTTCCCTTCCGGCAAATCAAGCGTGGCGAGCCGCAGGCCGGTATGACGATTCGCCCCGGCGTAGAGTAATTCGCTTTGCTGTACCGCCGTTTCCGGCGTGCCGCCGGGCAGGTTTCCAGACCAGCTTTCATGTGAGATAGCGGTGATTTTCCCGCTCTGGTCGGCACCGATACGCAAGTGCTGAAGGGTGGCGGGGCGGTGCGTGGTGTTATTGGGAATAGAGGGGCGGGGGAGCATCA  >  CP009273/295547‑295843

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑