Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 450,329 C→G 15.9% intergenic (+84/‑260) bolA → / → tig stationary‑phase morphogene, transcriptional repressor for mreB; also regulator for dacA, dacC, and ampC/peptidyl‑prolyl cis/trans isomerase (trigger factor)

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273450,3290CG15.9% 741.1 / 38.7 269intergenic (+84/‑260)bolA/tigstationary‑phase morphogene, transcriptional repressor for mreB; also regulator for dacA, dacC, and ampC/peptidyl‑prolyl cis/trans isomerase (trigger factor)
Reads supporting (aligned to +/- strand):  ref base C (56/161);  new base G (38/2);  total (106/163)
Fisher's exact test for biased strand distribution p-value = 3.16e-17
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 270 total aligned reads displayed.

GGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGT‑TTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGA‑CGAAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATGCCGCTATAATGCCGCGTCCTATTTGAATGCTTTCGGGATGATT  >  CP009273/450182‑450476
                                                                                                                                                    |                                                                                                                                                    
 gAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGT‑TTTGTTTTGTCTg                                                                                                                                                    <  1:2120811/149‑1 (MQ=255)
 gAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGT‑TTTGTTTTGTATg                                                                                                                                                    >  2:1078199/1‑149 (MQ=255)
 gAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGT‑GTTGTTTtgtgtg                                                                                                                                                    >  1:1672939/1‑149 (MQ=255)
   aGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGT‑TTTGTTTTGTCTGGa                                                                                                                                                  >  1:3311293/1‑149 (MQ=255)
    gggTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGT‑TTTGTTTTTTGTGGAt                                                                                                                                                 >  2:1531242/1‑149 (MQ=255)
    gggTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGT‑GTTGTTTTGTGTgggt                                                                                                                                                 >  1:3183372/1‑147 (MQ=255)
    gggTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGT‑GGTGTTTTTTGTGGAt                                                                                                                                                 >  1:1870083/1‑149 (MQ=255)
    gggTTGCAGGACACCGTATTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGT‑TTTGTTTTGTCTGGAt                                                                                                                                                 <  2:4479777/149‑1 (MQ=255)
      gTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGT‑TTTGTTTTTTATTGAttt                                                                                                                                               >  2:1826926/1‑149 (MQ=255)
       ttGCAGGACACCGTCTTGGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGT‑TTTGTTTTGTCTGGAtttt                                                                                                                                              <  2:3051120/149‑1 (MQ=255)
        tGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCTGGAAGCATCGCTTAAAAACGCTTTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAATATTTTATGAAAAACGGCCTGCGGGCCGT‑TTTGTTTTGTCTGGATTTTg                                                                                                                                             <  1:5736/149‑1 (MQ=255)
           aGGACACCGTCTTTGCCTCTCCTCCCTGTCGGGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGT‑TTTGTTTTGTCTGGATTTTgcgc                                                                                                                                          <  1:2878825/149‑1 (MQ=255)
            ggACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGT‑GTTGTTTTTTCTGGATTTTTCGCt                                                                                                                                         >  2:1722108/1‑149 (MQ=255)
            ggACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGG‑TGTGTTTTTTGTGGGtttttgggt                                                                                                                                         >  1:2425605/1‑144 (MQ=255)
             gACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGT‑TTTGTTTTGTCTGGATTTTGCGCtt                                                                                                                                        <  2:5082928/149‑1 (MQ=255)
                 ccGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGT‑ATTGTTTTGTCTTGTTTTTGAGCTTTTTg                                                                                                                                    >  2:4259132/1‑149 (MQ=255)
                 ccGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGGATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGT‑TTTGTTTTGTCTTGATTTTTCGCtttttt                                                                                                                                    >  2:4073055/1‑148 (MQ=255)
                  cGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCTTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGATAAAGATTTTATGAAAAACGGCCTGCGGGCCGT‑TTTGTTTTGTCTGGATTTTGCGCTTTTTGc                                                                                                                                   <  1:3925671/149‑1 (MQ=255)
                    tCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGT‑TTTGTTTTTTCTGGATTTTGAGCTTTTTGccc                                                                                                                                 >  2:4678437/1‑149 (MQ=255)
                     cTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGT‑GTTGTTTTTTGTGGTGTTTTAGGTTTTTGTCCa                                                                                                                                >  1:4166288/1‑149 (MQ=255)
                      tttGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGT‑TTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAg                                                                                                                               >  2:271047/1‑149 (MQ=255)
                      tttGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGT‑TGTGTTTTTTGTGGGTTTTTGGCTTTTTTGCCAg                                                                                                                               >  1:1586387/1‑149 (MQ=255)
                          ccTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTGTTTTTTTTTGGTGTGTTTTGGGTTTTTTGcccgcaaa                                                                                                                            >  1:1033806/1‑144 (MQ=255)
                          ccTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGT‑GTTGTTTTTTGTGGATTTTTAGCTTTTTTACCAGCAtt                                                                                                                           >  2:528338/1‑149 (MQ=255)
                           ctctCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGT‑TTTGTTTTTTGTGGATTTTTCGCTTTTTTCCCAGCATTc                                                                                                                          >  2:1893710/1‑149 (MQ=255)
                            tctcCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGT‑TTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCa                                                                                                                         <  2:2443379/149‑1 (MQ=255)
                              tcctccCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGT‑TTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGa                                                                                                                       <  2:5126458/149‑1 (MQ=255)
                              tcctccCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGT‑TTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGa                                                                                                                       <  1:3858566/149‑1 (MQ=255)
                               cctccCTGGCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGT‑TTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGA‑c                                                                                                                     <  1:2252721/149‑1 (MQ=255)
                                 tccCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGT‑TTTTTTTTGTCTGGTTTTTGCGCTTTTTGCCCCGCATTCAGA‑cca                                                                                                                   >  2:1058414/1‑147 (MQ=255)
                                     tGTCGTGGAGCTGTAAGCATCGCGTAAAAACGCATTGGCAACTGTCGGGGCTTTTCCAGTATGTTGCTAAAGATTTTTTTAAACACGGCCTGCGGGCCGT‑TTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGA‑CGAAAAt                                                                                                               <  1:3900474/149‑1 (MQ=255)
                                      gTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGT‑TTTTTTTTTTGTTGCTTTTTGGCTTTTTTGCCAGCAATAAGA‑CGAAAAtt                                                                                                              >  1:4611717/1‑149 (MQ=255)
                                      gTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGT‑TTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGA‑CGAAAAtt                                                                                                              <  1:4656277/149‑1 (MQ=255)
                                       tCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGT‑TGTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCCATCAGA‑CGAAAAttt                                                                                                             >  2:4527254/1‑148 (MQ=255)
                                        cGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGT‑TTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGA‑CGAAAATTGc                                                                                                            <  2:3205372/149‑1 (MQ=255)
                                         gTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGT‑TTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGA‑CGAAAATTGcc                                                                                                           <  1:1523409/149‑1 (MQ=255)
                                         gTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGG‑GTTGTTTTGTCTGGATTTTTCGCTTTTTGCCCAGCATTCAGA‑CGAAAATTGcc                                                                                                           >  2:253196/1‑149 (MQ=255)
                                           ggAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGT‑GTTGTTTTGTGTGGATTTTGCGCTTTTTGCCCACCAATCAGA‑CGCAAAATGcccc                                                                                                         >  2:3132603/1‑148 (MQ=255)
                                                aGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGT‑TTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGA‑CGAAAATTGCCCGGGAAt                                                                                                    <  2:2275585/149‑1 (MQ=255)
                                                     gCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGT‑TTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGA‑CGAAAATTGCCCGGGAATTGTGa                                                                                               <  2:233993/149‑1 (MQ=255)
                                                        tCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGT‑GTTGTTTTGTCTGGGTTTTGCGCTTTTTGCCCAGCATTCCGA‑CGAAAATTGCCCGGGGAATGTGtaaa                                                                                            >  2:3246046/1‑149 (MQ=255)
                                                          gcgTAAAACCTCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGT‑TTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGA‑CGAAAATTGCCCGGGAATTGTGaaaaaa                                                                                          <  2:4015892/149‑1 (MQ=255)
                                                          gcgTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAATCGGCCTGCGGGCCGG‑TTTGTTTTTTCTGGCGTTTGCGCTTTTTGCCCCGCATTAAGA‑CGAAAATTGGCCGGGTAATGTGaaaaaa                                                                                          >  2:1914503/1‑149 (MQ=255)
                                                             tAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAATATTTTATGAAAAACGGCCTGCGGGCCGT‑TTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGA‑CGAAAATTGCCCGGGAATTGTGAAAAAATAc                                                                                       <  1:1802362/149‑1 (MQ=255)
                                                             tAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGT‑TTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGA‑CGAAAATTGCCCGGGAATTGTGAAAAAATAc                                                                                       <  1:5174538/149‑1 (MQ=255)
                                                             tAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGATAAAGATTTTATGAAAAACGGCCTGCGGGCCGT‑TTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGA‑CGAAAATTGCCCGGGAATTGTGAAAAAATAc                                                                                       <  1:1802269/149‑1 (MQ=255)
                                                              aaaaaCGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGT‑TTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGA‑CGAAAATTGCCCGGGAATTGTGAAAAAATAcg                                                                                      <  2:4970237/149‑1 (MQ=255)
                                                                 aaCGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGT‑TTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGA‑CGAAAATTGCCCGGGAATTGTGAAAAAATACGCGa                                                                                   <  1:1213008/149‑1 (MQ=255)
                                                                   cGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGT‑TTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGA‑CGAAAATTGCCCGGGAATTGTGAAAAAATACGCGACa                                                                                 <  2:3848644/149‑1 (MQ=255)
                                                                       tttGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGT‑GTGGGTTTGTCTGGATTTTGCGCTTTTTGCCCCGCCATCAGA‑CGAAAAATGCCCGGGAATTGTGAAAAAAAAAGCGACCgcgc                                                                             >  2:4246445/1‑149 (MQ=255)
                                                                         tgaaagtGTCGGCGATTTTCCATTTTGTTTATAATTTTTTTTTTAAAAACGGCCTGGGGGCCTT‑TTTTTTTTGTGTGGATTTTGCGATTTTTGCCCAGCATTCATA‑CGAAATTTGCCCGGGAATTGTGAAAAAATACGCGACAgcgcgc                                                                           <  1:4421388/143‑1 (MQ=255)
                                                                               tGTCGGCGCTTGTCCCTTAGGTTGATAAAGATTTTATGAAAAACGGCCTGCGGGCCGT‑TTTTTTTTGTCTGGATTTGGCGCTTTTTGCCCAGCATTCAGA‑CGAAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAAc                                                                     <  2:4335004/149‑1 (MQ=255)
                                                                                     cgcTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGT‑TTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGA‑CGAAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTtct                                                               <  1:1499781/149‑1 (MQ=255)
                                                                                        ttttCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGT‑TTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGA‑CGAAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGa                                                            <  2:1587034/149‑1 (MQ=255)
                                                                                          ttCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGT‑TTTGTTTTGTCTGGATTTTGCGCTTTTTTCCCAGCATTCAGA‑CGAAAAATGCCCGGGAAATGTGAAAAAATACGCGACAGCGCGCAATAAACGTTCTCGACt                                                          >  2:266256/1‑149 (MQ=255)
                                                                                          ttCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGT‑GTTGTTTTTTCTGGAATTTTCGCATTTTTCCCACCCTTCCCA‑CCAAAAATTCCCGGGAGTTTTGAAAAAATAAGCGCCAGCGCGCAAAAACCGATCACGCCt                                                          >  2:1425530/1‑149 (MQ=255)
                                                                                            cctgTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCTT‑TTTTTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGA‑CGAAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCa                                                        <  1:3152400/146‑1 (MQ=255)
                                                                                              aGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGT‑TTTGTTTTGTGTGGATTTTTCGCTTTTTTCCCCCCAATCAGA‑AGAAAAATTCCCCGGAAATTTGTAAAAAAACAACACAGCGCGCCAAAAACGTTATCGAcacaaa                                                      >  1:2021024/1‑147 (MQ=255)
                                                                                               gTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGT‑TTTTTTTTTTATGGATTTTTAGCTTTTTTCCCACCAATAAGA‑AGAAAAATTACCGGGAAATGTGAAAAAAAAAGCGACAACGCGCAAAAAACGTTATCGACTCATaa                                                     >  1:3019909/1‑149 (MQ=255)
                                                                                                ttttttGCTAATTTTTTTATTAAAAACGGCCTGCGGGCCGT‑TTTTTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGA‑CTAAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATaaa                                                    <  1:3576790/145‑1 (MQ=255)
                                                                                                  tGTTGCTAATTATTTTATTAAAAACGGCCTGCGGGCCGT‑TTTGTTTTGTCTGTATTTTGCGCTTTTTGCCCAGAATTCAGA‑CGAAAATTGCCCGGGAATGGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAg                                                  <  1:4846657/149‑1 (MQ=255)
                                                                                                   tttGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGT‑TTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGA‑CGAAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGt                                                 <  2:720337/148‑1 (MQ=255)
                                                                                                   gTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGT‑TTTGTTTTTTGTGGATTTTTGGCTTTTTTCCCACCAATCAGA‑CGAAAAATTCCCGGGCCGTGTTAAAAAATAAACGACAGCCCGCACAAACCCTTCACGACACaaaaaaat                                                 >  1:3440410/1‑147 (MQ=255)
                                                                                                   gTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGT‑TTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGA‑CGAAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGt                                                 <  2:4575184/149‑1 (MQ=255)
                                                                                                    ttGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGT‑TTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCACCAATAAGA‑CCAAAAAAGCCCCGGCAATGATAAAAAAAAAACCACACCGCCCAAAAAACGATCTCGACACAAAAAATTg                                                >  2:1624815/1‑149 (MQ=255)
                                                                                                      gCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGT‑TTTGTTTTGTCTGTATTTTGCGCTTTTTGCCCAGCATTCAGA‑CGAAAATTGCCCGGGAATGGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGAt                                              <  1:1351432/149‑1 (MQ=255)
                                                                                                       cTAAAGATTTTATGAAAAACGGCCTGCGGGCCGT‑TTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGA‑CGAAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATg                                             <  2:3533708/149‑1 (MQ=255)
                                                                                                       ataattaTTTTATTAAAAACGGCCTGGGGGCCTT‑TTTGTTTTGTCTGTATTTTGCGCTTTTTGCCCAGCATTCAGA‑CGAAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATg                                             <  1:1406442/143‑1 (MQ=255)
                                                                                                        taaattttttATTAAAAACGGCCTGCGGGCCGT‑TTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGA‑CGAAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATGc                                            <  1:3721243/143‑1 (MQ=255)
                                                                                                        tAAAGATTTTATGAAAAACGGCCTGCGGGCCGT‑TTTGTTTTGTCGGGATTTTGCGCTTTTTGCCCAGCATTCAGA‑CGAAAATTTCCCGGGACTTGTGAAAAAATACGCGACAGCGCGCCAAAACCCTTCTCGACTCAAAAAAGAGATGc                                            >  2:3773272/1‑149 (MQ=255)
                                                                                                        tAAAGATTTTATGAAAAACGGCCTGCGGGCCGT‑GTTGTTTTTTGGGGGTTTTTGGCTTTTTTGCCCGCAATCAGACCCAAAATTCCCCGGG‑ACTGTGGAAAAAAACACGACCGCGCGCCATAACCCGGAGCGACTCAAAAAAATGAttc                                            >  1:3323913/1‑147 (MQ=255)
                                                                                                         aaaGATTTTATGAAAAACGGCCTGCGGGCCGT‑TTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGA‑CGAAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATGcc                                           <  2:2041050/149‑1 (MQ=255)
                                                                                                            gtttttATGAAAAACGGCCTGCGGGCCGT‑TTTGTTTTGTCTGTATTTTGCGATTTTTGCCCAGCATTCAGA‑CTAAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATGCCGCt                                        <  1:948345/147‑1 (MQ=255)
                                                                                                            gATTTTATGAAAAACGGCCTGCGGGCCGT‑TTTTTTTTGTCTTGGTTTTGCGCTTTTTTCCCAGCAGTCAGA‑CGAAAATTTCCCGGGAATTGTGTAAAAATACGCGCCAGCGCGCAATAACCGGTATCGACGCATAAAAGTGCTTCCGca                                        >  2:2836634/1‑148 (MQ=255)
                                                                                                             aTTTTATGAAAAACGGCCTGCGGGCCGT‑TTTGTTTTGTGTGGATTTTGCGCTTTTTGCCCAGCAATCAGA‑CGAAAAATGCCCCGGCAATGTGAAAAAATAAGCGACAGCGCGCAAAAACCGTTCTCGACACATAAAAGTGATTCCGcga                                       >  2:4511436/1‑147 (MQ=255)
                                                                                                             aTTTTATGAAAAACGGCCTGCGGGCCGT‑TTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGA‑CGAAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATGCCGCta                                       <  1:4707795/149‑1 (MQ=255)
                                                                                                              ttttATGAAAAACGGCCTGCGGGCCGT‑GTTTTTTTTTCTTGATTTTTGGCTTTTTTCCCACCATTAAGA‑CGAAAAATTCCCCGGAATTTTGAAAAAATAAACGACAGCGCGCCAAAAACCTTATAGAATCATAAAAATTATGCCGCtaa                                      >  1:4309637/1‑148 (MQ=255)
                                                                                                                    gAAAAACGGCCTGCGGGCCGT‑TTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGA‑CGAAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATGCCGCTATAATGcc                                <  2:4938756/149‑1 (MQ=255)
                                                                                                                      aaaaCGGCCTGCGGGCCGT‑TTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGA‑CGAAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATGCCGCTATAATGCcgc                              <  1:3829111/149‑1 (MQ=255)
                                                                                                                       aaaCGGCCTGCGGGCCGT‑TTTGTTTTGTCTTGATTTTGCGCTTTTTGCCCAGCAATCAGA‑CGAAAAATTCCCGGGCATTGTGAAAAAATACGCGACCGCGCGCAATAACCCTTCGCGACACAAAAAAGTGATTCCGCTATAATGCcgcg                             >  2:2972344/1‑149 (MQ=255)
                                                                                                                          cGGCCTGCGGGCCTT‑TTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGAATTCAGA‑CGAAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATGCCGCTATAATGCCGCGtac                          <  1:896610/149‑3 (MQ=255)
                                                                                                                          cGGCCTGCGGGCCGT‑TTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGA‑CGAAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATGCCGCTATAATGCCGCGTcc                          <  2:3587128/149‑1 (MQ=255)
                                                                                                                              cTGCGGGCCGT‑TTTGTTTTGTCTTGATTTTGCGCATTTTTCCCCGCAATCAGA‑CGAAAAATGCCCCGGAATTGTGAAAAAATACCCGACAGCGCGCAAAAACCGTTCTCGACTCAAAAAAGTGATGCCCCTATAATTCCGCGTACAAtt                      >  2:382563/1‑149 (MQ=255)
                                                                                                                              cTGCGGGCCGT‑TTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGA‑CGAAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATGCCGCTATAATGCCGCGTCCTAtt                      <  1:714364/149‑1 (MQ=255)
                                                                                                                               tGCGGGCCGT‑TTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGA‑CGAAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATGCCGCTATAATGCCGCGTCCTAttt                     <  1:5049237/149‑1 (MQ=255)
                                                                                                                                   ggCCGT‑TTTGTTTTGTCGGGATTTTGCGCTTTTTGCCCAGCATTCAGA‑CGAAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATGCCGCTATAATGCCGCGTCCTATTTGAAt                 <  2:2623565/149‑1 (MQ=255)
                                                                                                                                    gCCGT‑TTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGA‑CGAAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATGCCGCTATAATGCCGCGTCCTATTTGAATg                <  1:2704202/149‑1 (MQ=255)
                                                                                                                                      cGT‑TTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGA‑CGAAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATGCCGCTATAATGCCGCGTCCTATTTGAATGCt              <  1:2315834/149‑1 (MQ=255)
                                                                                                                                       gt‑tttgttttGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGA‑CGAAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATGCCGCTATAATGCCGCGTCCTATTTGAATGCtt             <  2:4166288/149‑1 (MQ=255)
                                                                                                                                       gt‑tttgttttGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGA‑CGAAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATGCCGCTATAATGCCGCGTCCTATTTGAATGCtt             <  1:5082352/149‑1 (MQ=255)
                                                                                                                                        t‑tttgttttgTGTGTATTTTGCGCTTTTTGCCCAGAATTCAGA‑CGAAAATTGCCCGGGATTTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATGCCGCTATAATGCCGCGTCCTATTTGAATGCttt            <  1:1296491/149‑1 (MQ=255)
                                                                                                                                        t‑tttgttttgTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGA‑CGAAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATGCCGCTATAATGCCGCGTCCTATTTGAATGCttt            >  2:3887880/1‑149 (MQ=255)
                                                                                                                                              ttttttCTGGATTTTGCGCTTTTTGCCCAGCATTCAGA‑CGAAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATGCCGCTATAATGCCGCGTCCTATTTGAATGCTTTCGGga       <  1:4568333/149‑1 (MQ=255)
                                                                                                                                              ttttgtCTGGATTTTGCGCTTTTTGCCCAGCATTCAGA‑CGAAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATGCCGCTATAATGCCGCGTCCTATTTGAATGCTTTCGGga       <  2:4109300/149‑1 (MQ=255)
                                                                                                                                              ttttgtCTGGATTTTGCGCTTTTTGCCCAGCATTCAGA‑CGAAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATGCCGCTATAATGCCGCGTCCTATTTGAATGCTTTCGGga       <  2:1493097/149‑1 (MQ=255)
                                                                                                                                              ttttgtCTGGATTTTGCGCTTTTTGCCCAGCATTCAGA‑CGAAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATGCCGCTATAATGCCGCGTCCTATTTGAATGCTTTCGGga       <  1:1078158/149‑1 (MQ=255)
                                                                                                                                               tttgtCTGGATTTTGCGCTTTTTGCCCAGCATTCAGA‑CGAAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATGCCGCTATAATGCCGCGTCCTATTTGAATGCTTTCGGgat      <  2:3347750/149‑1 (MQ=255)
                                                                                                                                                 tgtCTGGATTTTGCGCTTTTTGCCCAGCATTCAGA‑CGAAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATGCCGCTATAATGCCGCGTCCTATTTGAATGCTTTCGGgatga    <  1:3153638/149‑1 (MQ=255)
                                                                                                                                                  gtCTGGATTTTGCGCTTTTTGCCCAGCATTCAGA‑CGAAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATGCCGCTATAATGCCGCGTCCTATTTGAATGCTTTCGGgatgat   <  1:2594117/149‑1 (MQ=255)
                                                                                                                                                   tCTGGATTTTGCGCTTTTTGCCCAGCATTCAGA‑CGAAAATTGCCCGGGAATGGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATGCCGCTATAATGCCGCGTCCTATTTGAATGCTTTCGGGATGAtt  <  1:3246046/149‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
GGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGT‑TTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGA‑CGAAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATGCCGCTATAATGCCGCGTCCTATTTGAATGCTTTCGGGATGATT  >  CP009273/450182‑450476

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑