Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 772,664 A→C 14.0% K289N (AAA→AAC)  tolA → membrane anchored protein in TolA‑TolQ‑TolR complex

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273772,6640AC14.0% 453.4 / 18.0 189K289N (AAA→AAC) tolAmembrane anchored protein in TolA‑TolQ‑TolR complex
Reads supporting (aligned to +/- strand):  ref base A (136/21);  new base C (3/29);  total (139/50)
Fisher's exact test for biased strand distribution p-value = 1.81e-17
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 199 total aligned reads displayed.

AGAGAAAGCTGCAGCCGACAAAAAAGCAGCAGAAAAAGCGGCTGCTGAAAAGGCAGCAGCTGATAAGAAAGCAGCGGCAGAAAAAGCCGCCGCAGACAAAAAAGCGGCAGCGGCAAAAGCTGCAGCTGAAAAAGCCGCTGCAGCAAAAGCGGCCGCAGAGGCAGATGATATTTTCGGTGAGCTAAGCTCTGGTAAGAATGCACCGAAAACGGGGGGAGGGGCGAAAGGGAACAATGCTTCGCCTGCCGGGAGTGGTAATACTAAAAACAATGGCGCATCAGGGGCCGATATCAATA  >  CP009273/772517‑772812
                                                                                                                                                   |                                                                                                                                                    
agagAAAGCTGCAGCCGACAAAAAAGCAGCAGAAAAAGCGGCTGCTGAAAAGGCAGCAGCTGATAAGAAAGCAGCGGCAGAAAAAGCCGCCGCAGACAAAAAAGCGGCAGCGGCAAAAGCTGCAGCTGAAAAAGCCGCTGCAGCAAAAg                                                                                                                                                     >  1:3056248/1‑149 (MQ=255)
agagAAAGCTGCAGCCGACAAAAAAGCAGCAGAAAAAGCGGCTGCTGAAAAGGCAGCAGCTGATAAGAAAGCAGCGGCAGAAAAAGCCGCCGCAGACAAAAAAGCGGCAGCGGCAAAAGCTGCAGCTGAAAAAGCCGCTGCAGCAAAAg                                                                                                                                                     >  1:2590213/1‑149 (MQ=255)
 gagaAAGCTGCAGCCGACAAAAAAGCAGCAGAAAAAGCGGCTGCTGAAAAGGCAGCAGCTGATAAGAAAGCAGCGGCAGAAAAAGCCGCCGCAGACAAAAAAGCGGCAGCGGCAAAAGCTGCAGCTGAAAAAGCCGCTGCAGCAAAAGc                                                                                                                                                    >  2:3226927/1‑149 (MQ=255)
 gagaAAGCTGCAGCCGACAAAAAAGCAGCAGAAAAAGCGGCTGCTGAAAAGGCAGCAGCTGATAAGAAAGCAGCGGCAGAAAAAGCCGCCGCAGACAAAAAAGCGGCAGCGGCAAAAGCTGCAGCTGAAAAAGCCGCTGCAGCAAAAGc                                                                                                                                                    >  1:2912799/1‑149 (MQ=255)
  agaAAGCTGCAGCCGACAAAAAAGCAGCAGAAAAAGCGGCTGCTGAAAAGGCAGCAGCTGATAAGAAAGCAGCGGCAGAAAAAGCCGCCGCAGACAAAAAAGCGGCAGCGGCAAAAGCTGCAGCTGAAAAAGCCGCTGCAGCAAAAGCg                                                                                                                                                   >  1:5186055/1‑149 (MQ=255)
     aaGCTGCAGCCGACAAAAAAGCAGCAGAAAAAGCGGCTGCTGAAAAGGCAGCAGCTGATAAGAAAGCAGCGGCAGAAAAAGCCGCCGCAGACAAAAAAGCGGCAGCGGCAAAAGCTGCAGCTGAAAAAGCCGCTGCAGCACAAGCGGcc                                                                                                                                                >  2:5199957/1‑149 (MQ=255)
     aaGCTGCAGCCGACAAAAAAGCAGCAGAAAAAGCGGCTGCTGAAAAGGCAGCAGCTGATAAGAAAGCAGCGGCAGAAAAAGCCGCCGCAGACAAAAAAGCGGCAGCGGCAAAAGCTGCAGCTGAAAAAGCCGCTGCAGCAAAAGCGGcc                                                                                                                                                >  2:2588677/1‑149 (MQ=255)
      aGCTGCAGCCGACAAAAAAGCAGCAGAAAAAGCGGCTGCTGAAAAGGCAGCAGCTGATAAGAAAGCAGCGGCAGAAAAAGCCGCCGCAGACAAAAAAGCGGCAGCGGCAAAAGCTGCAGCTGAAAAAGCCGCTGCAGCAAAAGCGGCCg                                                                                                                                               >  2:1864014/1‑149 (MQ=255)
      aGCTGCAGCCGACAAAAAAGCAGCAGAAAAAGCGGCTGCTGAAAAGGCAGCAGCTGATAAGAAAGCAGCGGCAGAAAAAGCCGCCGCAGACAAAAAAGCGGCAGCGGCAAAAGCTGCAGCTGAAAAAGCCGCTGCAGCAAAAGCGGCCg                                                                                                                                               >  1:4056029/1‑149 (MQ=255)
      aGCTGCAGCCGACAAAAAAGCAGCAGAAAAAGCGGCTGCTGAAAAGGCAGCAGCTGATAAGAAAGCAGCGGCAGAAAAAGCCGCCGCAGACAAAAAAGCGGCAGCGGCAAAAGCTGCAGCTGAAAAAGCCGCTGCAGCAAAAGCGGCCg                                                                                                                                               >  2:2162902/1‑149 (MQ=255)
            aGCCGACAAAAAAGCAGCAGAAAAAGCGGCTGCTGAAAAGGCAGCAGCTGATAAGAAAGCAGCGGCAGAAAAAGCCGCCGCAGACAAAAAAGCGGCAGCGGCAATAGCTGCAGCTGAAAAAGCCGCTGCAGCAAAAGCGGCCGCAGAgg                                                                                                                                         >  2:4242269/1‑149 (MQ=255)
             gCCGACAAAAAAGCAGCAGAAAAAGCGGCTGCTGAAAAGGCAGCAGCTGATAAGAAAGCAGCGGCAGAAAAAGCCGCCGCAGACAAAAAAGCGGCAGCGGCAAAAGCTGCAGCTGAAAAAGCCGCTGCAGCAAAAGCGGCCGCAGAGGc                                                                                                                                        >  1:4650528/1‑149 (MQ=255)
             gCCGACAAAAAAGCAGCAGAAAAAGCGGCTGCTGAAAAGGCAGCAGCTGATAAGAAAGCAGCGGCAGAAAAAGCCGCCGCAGACAAAAAAGCGGCAGCGGCAAAAGCTGCAGCTGAAAAAGCCGCTGCAGCAAAAGCGGCCGCAGAGGc                                                                                                                                        >  2:3315298/1‑149 (MQ=255)
                  cAAAAAAGCAGCAGAAAAAGCGGCTGCTGAAAAGGCAGCAGCTGATAAGAAAGCAGCGGCAGAAAAAGCCGCCGCAGACAAAAAAGCGGCAGCGGCAAAAGCTGCAGCTGAAAAAGCCGCTGCAGCAAAAGCGGCCGCAGAGGCAgatg                                                                                                                                   >  1:1822447/1‑149 (MQ=255)
                        agcagcAGAAAAAGCGGCTGCTGAAAAGGCAGCAGCAGATAAGAAAGCAGCGGCAGAAAAAGCCGCCGCAGACAAAAAAGCGGCACCGGCAAAAGCTGCAGCTGAAAAAGCCGCTGCAGCAAAAGCGGCCGCAGAGGCAGATGatatat                                                                                                                             >  2:412052/1‑147 (MQ=255)
                          ctgattaaaagGGGGCTGATTAAACGGCAGCTGCTTAAACTAACGCAGCTGCACAACACCCCCCCGCAGACAAAAAAGCGGCTGCGGAAAACGTTGCAGCTGAAAACGCCGGTGCAGAAAACGCGGCCGCAGAGGCAGATGATATTTTc                                                                                                                           <  2:3758264/143‑1 (MQ=255)
                            gcagAAAAAGCGGCTGCTGAAAAGGCAGCAGCTGATACGAAAGCAGCGGCAGAAAAAGCCGCCGCAGACAAAAAAGCGGCAGCGGCAAAAGCTGCAGCTGAAAGAGCCGCTGCAGCAAAAGCGGCCGCAGAGGCAGAGGAAATTTTCgg                                                                                                                         >  2:2096511/1‑149 (MQ=255)
                            gcagAAAAAGCGGCTGCTGAAAAGGCAGCAGCTGATAAGAAAGCAGCGGCAGAAAAAGCCGCCGCAGACAAAAAAGCGGCAGCGGCAAAAGCTGCAGCTGAAAAAGCCGCTGCAGCAAAAGCGGCCGCAGAGGCAGATGATATTTTCgg                                                                                                                         >  2:4965122/1‑149 (MQ=255)
                            ccataaaatGGGGATGCTGAACCGGCTGCGGCTTTTACGAACGCTGCGGCAGAAAACGCCGCCGCAGACAAAAACGCGGCAGCGGCAAAAGCTGCAGCTGAAAACGCCGCTGCAGAAAAAGCGGCCGCAGAGGCAGATGATATTTTCgg                                                                                                                         <  2:2153292/145‑1 (MQ=255)
                              agAAAAAGCGGCTGCTGAAAAGGCAGCAGCTGATAAGAAAGCAGCGGCAGAAAAAGCCGCCGCAGACAAAAAAGCGGCAGCGGCAAAAGCTGCAGCTGAAAAAGCCGCTGCAGCAAAAGCGGCCGCAGAGGCAGATGATATTTTCGGTg                                                                                                                       >  2:1788987/1‑149 (MQ=255)
                                 aaaaGCGGCTGCTGAAAAGGCAGCAGCTGATAAGAAAGCAGCGGCAGAAAAAGCCGCCGCAGACAAAAAAGCGGCAGCGGCAAAAGCTGCAGCTGAAAAAGCCGCTGCAGCAAAAGCGGCCGCAGAGGCAGATGATATTTTCGGTGAGc                                                                                                                    >  2:2229485/1‑149 (MQ=255)
                                 aaaaGCGGCTGCTGAAAAGGCAGCAGCTGATAAGAAAGCAGCGGCAGAAAAAGCCGCCGCAGACAAAAAAGCGGCAGCGGCAAAAGCTGCAGCTGAAAAAGCCGCTGCAGCAAAAGCGGCCGCAGAGGCAGATGATATTTTCGGTGAGc                                                                                                                    >  2:648850/1‑149 (MQ=255)
                                    aGCGGCTGCTGAAAAGGCAGCAGCTGATAAGAAAGCAGCGGCAGAAAAAGCCGCCGCAGACAAAAAAGCGGCAGCGGCAAAAGCTGCAGCTGAAAAAGCCGCTGCAGCAAAAGCGGCCGCAGAGGCAGATGATATTTTCGGTGAGCTaa                                                                                                                 >  1:4187287/1‑149 (MQ=255)
                                    aGCGGCTGCTGAAAAGGCAGCAGCTGATAAGAAAGCAGCGGCAGAAAAAGCCGCCGCAGACAAAAAAGCGGCAGCGGCAAAAGCTGCAGCTGAAAAAGCCGCTGCAGCAAAAGCGGCCGCAGAGGCAGATGATATTTTCGGTGAGCTaa                                                                                                                 >  1:4188046/1‑149 (MQ=255)
                                     gCGGCTGCTGAAAAGGCAGCAGCTGATAAGAAAGCAGCGGCAGAAAAAGCCGCCGCAGACAAAAAAGCGGCAGCGGCAAAAGCTGCAGCTGAAAAAGCCGCTGCAGCAAAAGCGGCCGCAGAGGCAGATGATATTTTCGGTGAGCTAAg                                                                                                                >  1:4966162/1‑149 (MQ=255)
                                     gCGGCTGCTGAAAAGGCAGCAGCTGATAAGAAAGCAGCGGCAGAAAAAGCCGCCGCAGACAAAAAAGCGGCAGCGGCAAAAGCTGCAGCTGAAAAAGCCGCAGCAGCAAAAGCGGCCGCAGAGGCAGATGATATTTTCGGTGAGCTAAg                                                                                                                >  1:3403337/1‑149 (MQ=255)
                                      cGGTTGCTGAAACGGCCGCAGCTGATACGAACGCTGCGGAATAAACCGCCGCCGCAGACAAAAAAGCGGCTGCGGCAACCGCGGCTGCTGAAAACGCCGCTGCAGCAAAAGCGGCCGCAGAGGCAGATGATATTTTCGGTGAGCTAAGc                                                                                                               <  2:3837373/149‑1 (MQ=255)
                                        gctgttTAAATGGCTGCAGCTTATAAGAAAGCCGCGGCATAAACAGCCGCCGCAGAAAAAAAAGCGGCAGCGGCAACAGCTGCTGCTTAAAAAGCCGCTGCTGCAAAAGCGGCCGCAGAGGCAGATGATATTTTCGGTGAGCTAAGctc                                                                                                             <  2:5171802/149‑1 (MQ=255)
                                           gctgAAAAGGCAGCAGCTGATAAGAAAGCAGCGGCAGAAAAAGCCGCCGCAGACAAAAAAGCGGCAGCGGCAAAAGCTGCAGCTGAAAAAGCCGCTGCAGCAAAAGCGGCCGCAGAGGCAGATGATATTTTCGGTGAGCTAAGCTCTgg                                                                                                          >  1:3667612/1‑149 (MQ=255)
                                             tgAAAAGGCAGCAGCTGATAAGAAAGCAGCGGCAGAAAAAGCCGCCGCAGACAAAAAAGCGGCAGCGGCAAAAGCTGCAGCTGAAAAAGCCGCTGCAGCAAAAGCGGCCGCAGAGGCAGATGATATTTTCGGTGAGCTAAGCTCTGGTa                                                                                                        >  2:890674/1‑149 (MQ=255)
                                               aaaaGGCAGCAGCTGATAAGAAAGCAGCGGCAGAAAAAGCCGCCGCAGACAAAAAAGCGGCAGCGGCAAAAGCTGCAGCTGAAAAAGCCGCTGCAGCAAAAGCGGCCGCAGAGGCAGATGATATTTTCGGTGAGCTAAGCTCTGGTAAg                                                                                                      >  1:1355282/1‑149 (MQ=255)
                                                      agcagcTGATAAGAAAGCAGCGGCAGAAAAAGCCGCCGCAGACAAAAAAGCGGCAGCGGCAAAAGCTGCAGCTGAAAAAGCCGCTGCAGCAAAAGCGGCCGCAGAGGCAGATGATATTTTCGGTGAGCTAAGCTCTGGTAAGAATGCAc                                                                                               >  1:4284954/1‑149 (MQ=255)
                                                       gcagcTGATAAGAAAGCAGCGGCAGAAAAAGCCGCCGCAGACAAAAAAGCGGCAGCGGCAAAAGCTGCAGCTGAAAAAGCCGCTGCAGCAAAAGCGGCCGCAGAGGCAGATGATATTTTCGGTGAGCTAAGCTCTGGTAAGAATGCAcc                                                                                              >  1:4625667/1‑149 (MQ=255)
                                                       gcagcTGATAAGAAAGCAGCGGCAGAAAAAGCCGCCGCAGACAAAAAAGCGGCAGCGGCAAAAGCTGCAGCTGAAAAAGCCGCTGCAGCAAAAGCGGCCGCAGAGGCAGATGATATTTTCGGTGAGCTAAGCTCTGGTAAGAATGCAcc                                                                                              >  1:3440902/1‑149 (MQ=255)
                                                       gcagcTGATAAGAAAGCAGCGGCAGAAAAAGCCGCCGCAGACAAAAAAGCGGCAGCGGCAAAAGCTGCAGCTGAAAAAGCCGCTGCAGCAAAAGCGGCCGCAGAGGCAGATGATATTTTCGGTGAGCTAAGCTCTGGTAAGAATGCAcc                                                                                              >  1:4625743/1‑149 (MQ=255)
                                                       gcagcTGATAAGAAAGCAGCGGCAGAAAAAGCCGCCGCAGACAAAAAAGCGGCAGCGGCAAAAGCTGCAGCTGAAAAAGCCGCTGCAGCAAAAGCGGCCGCAGAGGCAGATGATATTTTCGGTGAGCTAAGCTCTGGTAAGAATGCAcc                                                                                              >  1:4535632/1‑149 (MQ=255)
                                                        ccgcTGATACTAACGCCGCGGCATAAAACGCCGCCTCATAAAAAAAAGCGGCTGCGGCAAAAGCTGCAGCTGAAAAAGCCGCTGCAGCAACAGCGGCCGCAGAGGCAGATGATATTTTCGGTGAGCTAAGCTCTGGTAAGAATGCACCg                                                                                             <  2:5128770/147‑1 (MQ=255)
                                                         agcTGATAAGAAAGCAGCGGCAGAAAAAGCCGCCGCAGACAAAAAAGCGGCAGCGGCAAAAGCTGCAGCTGAAAAAGCCGCTGCAGCAAAAGCGGCCGCAGAGGCAGATGATATTTTCGGTGAGCTACGCTCTGGTAAGAATGCACCGa                                                                                            >  1:3279948/1‑149 (MQ=255)
                                                          gcTGATAAGAAAGCAGCGGCAGAAAAAGCCGCCGCAGACAAAAAAGCGGCAGCGGCAAAAGCTGCAGCTGAAAAAGCCGCTGCAGCAAAAGCGGCCGCAGAGGCAGATGATATTTTCGGTGAGCTAAGCTCTGGTAAGAATGCACCGaa                                                                                           >  2:3434118/1‑149 (MQ=255)
                                                           cTGATAAGAAAGCAGCGGCAGAAAAAGCCGCCGCAGACAAAAAAGCGGCAGCGGCAAAAGCTGCAGCTGAAAAAGCCGCTGCAGCACAAGCGGCCGCAGAGGCAGATGATATTTTCGGTGAGCTAAGCTCTGGTAAGAATGCACCGaaa                                                                                          >  2:351209/1‑149 (MQ=255)
                                                             gATAAGAAAGCAGCGGCAGAAAAAGCCGCCGCAGACAAAAAAGCGGCAGCGGCAAAAGCTGCAGCTGAAAAAGCCGCTGCAGCAAAAGCGGCCGCAGAGGCAGATGATATTTTCGGTGAGCTAAGCTCTGGTAAGAATGCACCGAAAAc                                                                                        >  2:1653217/1‑149 (MQ=255)
                                                              aTAAGAAAGCAGCGGCAGAAAAAGCCGCCGCAGACAAAAAAGCGGCAGCGGCAAAAGCTGCAGCTGAAAAAGCCGCTGCAGCACAAGCGGCCGCAGAGGCAGATGATATTTTCGGTGAGCTAAGCTCTGGTAAGAATGCACCGAAAACg                                                                                       >  1:4382654/1‑149 (MQ=255)
                                                              aTAAGAAAGCAGCGGCAGAAAAAGCCGCCGCAGACAAAAAAGCGGCAGCGGCAAAAGCTGCAGCTGAAAAAGCCGCTGCAGCAAAAGCGGCCGCAGAGGCAGATGATATTTTCGGTGAGCTAAGCTCTGGTAAGAATGCACCGAAAACg                                                                                       >  1:702058/1‑149 (MQ=255)
                                                               tAAGAAAGCCGCGGCATAAAACGCCGCCGCATACAAAAAAGCGGCAGCGGCAAACGCTGCAGATGAAAAAGCCGCTGCAGAAAAAGCGGCCGCAGAGGCAGATGATATTTTCGGTGAGCTAAGCTCTGGTAAGAATGCACCGAAAACgg                                                                                      <  2:3871339/149‑1 (MQ=255)
                                                               tAAGAAAGCAGCGGCAGAAAAAGCCGCCGCAGACAAAAAAGCGGCAGCGGCAAAAGCTGCAGCTGAAAAAGCCGCTGCAGCAAAAGCGGCCGCAGAGGCAGATGATATTTTCGGTGAGCTAAGCTCTGGTAAGAATGCACCGAAAACgg                                                                                      >  1:3070895/1‑149 (MQ=255)
                                                                  gAAAGCAGCGGCAGAAAAAGCCGCCGCAGACAAAAAAGCGGCAGCGGCAAAAGCTGCAGCTGAAAAAGCCGCTGCAGCAAAAGCGGCCGCAGAGGCAGATGATATTTTCGGTGAGCTAAGCTCTGGTAAGAATGCACCGAAAACggggg                                                                                   >  2:152142/1‑149 (MQ=255)
                                                                  gAAAGCAGCGGCAGAAAAAGCCGCCGCAGACAAAAAAGCGGCAGCGGCAAAAGCTGCAGCTGAAAAAGCCGCTGCAGCAAAAGCGGCCGCAGAGGCAGATGATATTTTCGGTGAGCTAAGCTCTGGTAAGAATGCACCGAAAACggggg                                                                                   >  2:350739/1‑149 (MQ=255)
                                                                    aaGCAGCGGCAGAAAAAGCCGCCGCAGACAAAAAAGCGGCAGCGGCAAAAGCTGCAGCTGAAAAAGCCGCTGCAGCAAAAGCGGCCGCAGAGGCAGATGATATTTTCGGTGAGCTAAGCTCTGGTAAGAATGCACCGAAAACGGGGGGa                                                                                 >  2:1550234/1‑149 (MQ=255)
                                                                     agcagcGGCAGAAAAAGCCGCCGCAGACAAAAAAGCGGCAGCGGCAAAAGCTGCAGCTGAAAAAGCCGCTGCAGCAAAAGCGGCCGCAGAGGCAGATGATATTTTCGGTGAGCTAAGCTCTGGTAAGAATGCACCGAAAACggggggcg                                                                                >  2:2033559/1‑147 (MQ=255)
                                                                     agcagcGGCAGAAAAAGCCGCCGCAGACAAAAAAGCGGCAGCGGCAAAAGCTGCAGCTGAAAAAGCCGCTGCAGCAAAAGCGGCCGCAGAGGCAGATGATATTTTCGGTGAGCTAAGCTCTGGTAAGAATGCACCGAAAACGGGGGGAg                                                                                >  1:152187/1‑149 (MQ=255)
                                                                       cagcGGCAGAAAAAGCCGCCGCAGACAAAAAAGCGGCAGCGGCAAAAGCTGCAGCTGAAAAAGCCGCTGCAGCAAAAGCGGCCGCAGAGGCTGATGATATTTTCGGTGAGCTAAGCTCTGGGAAGAATGCACCGAAAACGGGGGGAggg                                                                              >  2:2278536/1‑149 (MQ=255)
                                                                       cagcGGCAGAAAAAGCCGCCGCAGACAAAAAAGCGGCAGCGGCAAAAGCTGCAGCTGAAAAAGCCGCTGCAGCAAAAGCGGCCGCAGAGGCAGATGATATTTTCGGTGAGCTAAGCTCTGGTAAGAATGCACCGAAAACGGGGGGAggg                                                                              >  2:1415525/1‑149 (MQ=255)
                                                                        agcGGCAGAAAAAGCCGCCGCAGACAAAAAAGCGGCAGCGGCAAAAGCTGCAGCTGAAAAAGCCGCTGCAGCAAAAGCGGCCGCAGAGGCAGATGATATTTTCGGTGAGCTAAGCTCTGGTAAGAATGCACCGAAAACGGGGGGAgggg                                                                             >  2:406921/1‑149 (MQ=255)
                                                                            gCAGAAAAAGCCGCCGCAGACAAAAAAGCGGCAGCGGCAAAAGCCGCAGCTGAAAAAGCCGCTGCAGCAAAAGCGGCCGCAGAGGCAGATGATATTTTCGGTGAGCTAAGCTCTGGTAAGAATGCACCGAAAACGGGGGGAGGGGCGaa                                                                         >  2:271542/1‑149 (MQ=255)
                                                                                aaaaaGACGCAGCAGACAAAAAAGCGGAAGCGGCAAAAGCTGCAGCTCAAAAAGCCGCACAAGCAAAAGCTGCCCCAGAGGCAGATTATATTTACGGTTAGCTAAGCTCTGGTAATAATGCACCCAAAACGGGGGGAGGGGCTAAAggg                                                                     >  2:3571858/1‑149 (MQ=255)
                                                                                      ccgccgCAGACAAAAACGGGGCTGGGGCAAACGCGGCTGCTGAAAACGCCGCTGCAGCAAACGCGGCCGCAGAGGCAGCTGATATTTTCGGTGAGCTAAGCTCTGGTAAGAATGCACCGAAAACGGGGGGAGGGGCGAAAGGGAACAAt                                                               <  2:2989324/149‑1 (MQ=255)
                                                                                         cctcAGACAAAAACGCGGCAGCGGCAAAAGCTCCTGCTTAAAAAGCCGCTGCATCAAAAGCGGCCGCAGAGGCAGATGATATTTTCGGTGAGCTAAGCTCTGGTAAGAATGCACCGAAAACGGGGGGAGGGGCGAAAGGGAACAATGCt                                                            <  2:5177629/146‑1 (MQ=255)
                                                                                         ccgcACACAACAAGGCGGCAGCGGCAAACGGTGCAGCTTAAACCGCGGCTGCTGCAAAAGCGGCCGCAGAGGCAGATGATATTTTCGGTGAGCTAAGCTCTGGTAAGAATGCACCGAAAACGGGGGGAGGGGCGAAAGGGAACAATGCt                                                            <  2:3848445/149‑1 (MQ=255)
                                                                                         ccccAGACAAAAACGGGGCCGCGCAAAATGCTGCTGCTTAAAAAGCCGCTGCAGAAAACGCGGCCGCAGAGGCAGATGATATTTTCGGGGAGCTAAGCTCTGGTAAGAATGCACCGAAAACGGGGGGAGGGGCGAAAGGGAACAATGCt                                                            <  2:5062612/146‑1 (MQ=255)
                                                                                            cAGACAAAAAAGCGGCAGCGGCAAAAGCTGCAGCTGAAAAAGCCGCTGCAGCAAAAGCGGCCGCAGAGGCAGATGATATTTTCGGTGAGCTAAGCTCTGGTAAGAATGCACCGAAAACGGGGGGAGGGGCGAAAGGGAACAATGCTTCg                                                         >  2:4065445/1‑149 (MQ=255)
                                                                                                  aaaaaGCGGCAGCGGCAAAAGCTGCAGCTGAAAAAGCCGCTGCAGCAAAAGCGGCCGCAGAGGCAGATGATATTTTCGGTGAGCTAAGCTCTGGTACGAATGCACCGAAAACGGGGGGAGGGGCGAAAGGGAACAATGCTTCGCCTGcc                                                   >  2:1722935/1‑149 (MQ=255)
                                                                                                  aaaaaGCGGCAGCGGCAAAAGCTGCAGCTGAAAAAGCCGCTGCAGCAAAAGCGGCCGCAGAGGCAGATGATATTTTCGGTGAGCTAAGCTCTGGTAAGAATGCACCGAAAACGGGGGGAGGGGCGAAAGGGAACAATGCTTCGCCTGcc                                                   >  2:2174249/1‑149 (MQ=255)
                                                                                                  aaaaaGCGGCAGCGGCAAAAGCTGCAGCTGAAAAAGCCGCTGCAGCAAAAGCGGCCGCAGAGGCAGATGATATTTTCGGTGAGCTAAGCTCTGGTAAGAATGCACCGAAAACGGGGGGAGGGGCGAAAGGGAACAATGCTTCGCCTGcc                                                   >  2:2174346/1‑149 (MQ=255)
                                                                                                   aaaaGCGGCAGCGGCAAAAGCTGCAGCTGAAAAAGCCGCTGCAGCAAAAGCGGCCGCAGAGGCAGATGATATTTTCGGTGAGCTAAGCTCTGGTAAGAATGCACCGAAAACGGGGGGAGGGGCGAAAGGGAACAATGCTTCGCCTGCCg                                                  >  2:1893002/1‑149 (MQ=255)
                                                                                                     aaGCGGCAGCGGCAAAAGCTGCAGCTGAAAAAGCCGCTGCAGCAAAAGCGGCCGCAGAGGCAGATGATATTTTCGGTGAGCTCAGCTCTGGTAAGAATGCACCGAAAACGGGGGGAGGGGCGAAAGGGAACAATGCTTCGCCTGCCggg                                                >  2:1262842/1‑149 (MQ=255)
                                                                                                     aaGCGGCAGCGGCAAAAGCTGCAGCTGAAAAAGCCGCTGCAGCAAAAGCGGCCGCAGAGGCAGATGATATTTTCGGTGAGCTAAGCTCTGGTAAGAATGCACCGAAAACGGGGGGAGGGGCGACAGGGAACAATGCTTCGCCTGCCggg                                                >  2:1262874/1‑149 (MQ=255)
                                                                                                     aaGCGGCAGCGGCAAAAGCTGCAGCTGAAAAAGCCGCTGCAGCAAAAGCGGCCGCAGAGGCAGATGATATTTTCGGTGAGCTAAGCTCTGGTAAGAATGCACCGAAAACGGGGGGAGGGGCGAAAGGGAACAATGCTTCGCCTGCCggg                                                >  1:21858/1‑149 (MQ=255)
                                                                                                       ggggcagcggaaAACGCTGCTGCTTAAAAAGCCGATGCAGCAAAAGCGGCCGCAGAGGCAGATTATATTTTCGTTGAGCTAAGCTATGGTAAGAATGCACCGAAAACGGGGGGAGGGGCGAAAGGGAACAATGCTTCGCCTGCCGGGAg                                              <  2:419705/147‑1 (MQ=255)
                                                                                                          gcagcggcaAAAGCTGCAGCTGAAAAAGCCGCTGCAGCAAAAGCGGCCGCAGAGGCAGATGATATTTTCGGTGAGCTCAGCTCTGGTAAGAATGCACCGAAAACGGGGGGAGGGGCGAAAGGGACCAATGCTTCGCCTGCCGGGAGTgg                                           >  2:3965516/1‑149 (MQ=255)
                                                                                                          gcagcggcaAAAGCTGCAGCTGAAAAAGCCGCTGCAGCAAAAGCGGCCGCAGAGGCAGATGATATTTTCGGTGAGCTAAGCTCTGGTAAGAATGCACCGAAAACGGGGGGAGGGGCGAAAGGGAACAATGCTTCGCCTGCCGGGAGTgg                                           >  1:1899996/1‑149 (MQ=255)
                                                                                                          gcagcggcaAAAGCTGCAGCTGAAAAAGCCGCTGCAGCAAAAGCGGCCGCAGAGGCAGATGATATTTTCGGTGAGCTAAGCTCTGGTAAGAATGCACCGAAAACGGGGGGAGGGGCGAAAGGGAACAATGCTTCGCCTGCCGGGAGTgg                                           >  1:4257843/1‑149 (MQ=255)
                                                                                                             gcggcaAAAGCTGCAGCTGAAAAAGCCGCTGCAGCAAAAGCGGCCGCAGAGGCAGATGATATTTTCGGTGAGCTAAGCTCTGGTAAGAATGCACCGAAAACGGGGGGAGGGGCGAAAGGGAACAATGCTTCGCCTGCCGGGAGTGGTaa                                        >  1:3846939/1‑149 (MQ=255)
                                                                                                                 aaaacGCTGCAGCTGAACACGCCGCGGCCGCAAAAGCGGCCGCAGTGGCTGATGATATTTTCGGGGAGATAAGCTCTGGTAAGAATGCACCGAAAACGGGGGGAGGGGCGAAAGGGAACAATGCTTCGCCTGCCGGGAGTGGTAATACt                                    <  2:5076076/148‑1 (MQ=255)
                                                                                                                   aaaGCTGCAGCTGAAAAAGCCGCTGCAGCAAAAGCGGCCGCAGAGGCAGATGATATTTTCGGTGAGCTAAGCTCTGGTAAGAATGCACCGAAAACGGGGGGAGGGGCGAAAGGGAACAATGCTTCGCCTGCCGGGAGTGGTAATACTaa                                  >  2:3149340/1‑149 (MQ=255)
                                                                                                                    acgccgCAGCTAAAAACGCCGGTCCACAAAACGCGCCCGAAGAGGCAGATGATATTTTCGGTGAGCTAAGCTCTGTTAAGAATGCACCGAAAACGGGGGGAGGGGCGAAAGGGAACAATGCTTCGCCTGCCGGGAGTGGTAATACTaaa                                 <  2:149974/144‑1 (MQ=255)
                                                                                                                    aaGCTGCAGCTGAAAAAGCCGCTGCAGCAAAAGCGGCCGCAGAGGCAGATGATATTTTCGGTGAGCTAAGCTCTGGTAAGAATGCACCGAACACGGGGGGAGGGGCGAAAGGGAACAATGCTTCGCCTGCCGGGAGTGGTAATACTaaa                                 >  2:5117585/1‑149 (MQ=255)
                                                                                                                    aaGCTGCAGCTGAAAAAGCCGCTGCAGCAAAAGCGGCCGCAGAGGCAGATGATATTTTCGGTGAGCTAAGCTCTGGTAAGAATGCACCGAAAACGGGGGGAGGGGCGAAAGGGAACAATGCTTCGCCTGCCGGGAGTGGTAATACTaaa                                 >  2:2074295/1‑149 (MQ=255)
                                                                                                                      gctgctgctTAAAAAGCCGCTGCATAAAACGCGGCCGCAGAGGCAGATGATATTTTCGGTGAGCTAAGCTCTGGTAAGAATGCACCGAAAACGGGGGGAGGGGCGAAAGGGAACAATGCTTCGCCTGCCGGGAGTGGTAATACTaaaaa                               <  2:306996/149‑1 (MQ=255)
                                                                                                                      gCTGCAGCTGAAAAAGCCGCTGCAGCAAACGCGGCCGCAGAGGCAGATGATATTTTCGGTGAGCTAAGCTCTGGTAAGAATGCACCGAAAACGGGGGGAGGGGCGAAAGGGAACAATGCTTCGCCTGCCGGGAGTGGTAATACTaaaaa                               >  1:1703754/1‑149 (MQ=255)
                                                                                                                      gCTGCAGCTGAAAAAGCCGCTGCAGCAAAAGCGGCCGCAGAGGCAGATGATATTTTCGGTGAGCTAAGCTCTGGTAAGAATGCACCGAAAACGGGGGGAGGGGCGAAAGGGAACAATGCTTCGCCTGCCGGGAGTGGTAATACTaaaaa                               >  2:1547432/1‑149 (MQ=255)
                                                                                                                         gccgCTTAAAACGCCGCTGCAGCAAAAGCGGCCGCAGAGGCAGATGATATTTTCGGTGAGCTAAGCTCTGGTAAGAATGCACCGAAAACGGGGGGAGGGGCGAAAGGGAACAATGCTTCGCCTGCCGGGAGTGGTAATACTAAAAACaa                            <  2:2516755/146‑1 (MQ=255)
                                                                                                                         gCAGCTGAAAAAGCCGCTGCAGCAAAAGCGGCCGCAGAGGCAGATGATATTTTCGGTGAGCTAAGCTCTGGTAAGAATGCACCGAAAACGGGGGGAGGGGCGAAAGGGAACAATGCTTCGCCTGCCGGGAGTGGTAATACTAAAAACaa                            >  2:3281619/1‑149 (MQ=255)
                                                                                                                         ccacctaaaaaaGCCCCGGCACCAAACGCGGCCGCAGCGGCAGATGATATTTTCGGTGAGCTAAGCTCTGGTAAGAATGCACCGAAAACGGGGGGAGGGGCGAAAGGGAACAATGCTTCGCCTGCCGGGAGTGGTAATACTAAAAACaa                            <  2:2306468/142‑1 (MQ=255)
                                                                                                                          cAGCTCAAACCGCCGCTGCACAAAACGCGGCCGCAGTGGCATATTATATTTTCGGTGAGCTAAGCTCTGGTAAGAATGCACCGAAAACGGGGGGAGGGGCGAAAGGGAACAATGCTTCGCCTGCCGGGAGTGGTAATACTAAAAACAAt                           <  1:3796337/149‑1 (MQ=255)
                                                                                                                            tgttaaaacGCCGCTGCATAAAACGCGGCCGCAGAGGCATATTATATTTTCGGTGAGCTAAGCTCTGGTAATAATGCACCTAAAACGGGGGGAGGGGCGAAAGGGAACAATGCTTCGCCTGCCGGGAGTGGTAATACTAAAAACAATgg                         <  2:2299176/145‑1 (MQ=255)
                                                                                                                            gattaaaacGCCGCTGCAGCAAAAGCGGCCGCAGAGGCAGATGATATTTTCGGTGAGCTAAGCTCTGTTAAGAATGCACCGAAAACGGGGGGAGGGGCGAAAGGGAACAATGCTTCGCCTGCCGGGAGTGGTAATACTAAAAACAATgg                         <  2:2435298/145‑1 (MQ=255)
                                                                                                                            gCTTAAAACGCCGCTCCACAAAACGCGGCCGCAGAGGCAGATTATATTTTCGGTGAGCTAAGCTCTGGTAAGAATGCACCGAAAACGGGGGGAGGGGCGAAAGGGAACAATGCTTCGCCTGCCGGGAGTGGTAATACTAAAAACAATgg                         <  2:1577852/149‑1 (MQ=255)
                                                                                                                               taaaaaGCCGCTGCAGAAACCGCGGCCGCAGAGGCAGATGATATTTTCGGTGAGCTAAGCTCTGGTAAGAATGCACCGAAAACGGGGGGAGGGGCGAAAGGGAACAATGCTTCGCCTGCCGGGAGTGGTAATACTAAAAACAATGgcgc                      <  2:4535632/148‑1 (MQ=255)
                                                                                                                                 aaaccccGCGGCACAAAACGCGGCCGCAGAGGCAGATGATATTTTCGGTGAGCTAAGCTCTGGTAAGAATGCACCGAAAACGGGGGGAGGGGCGAAAGGGAACAATGCTTCGCCTGCCGGGAGTGGTAATACTAAAAACAATGGCGCAt                    <  2:4705417/144‑1 (MQ=255)
                                                                                                                                   aaGCCGCTGCAGCAAAAGCGGCCGCAGAGGCAGATGATATTTTCGGTGAGCTAAGCTCTGGTAAGAATGCACCGAAAACGGGGGGAGGGGCGAAAGGGAACAATGCTTCGCCTGCCGGGAGTGGTAATACTAAAAACAATGGCGCATCa                  >  1:1286465/1‑149 (MQ=255)
                                                                                                                                   aaGCCGCTGCAGCAAAAGCGGCCGCAGAGGCAGATGATATTTTCGGTGAGCTAAGCTCTGGTAAGAATGCACCGAAAACGGGGGGAGGGGCGAAAGGGAACAATGCTTCGCCTGCCGGGAGTGGTAATACTAAAAACAATGGCGCATCa                  >  1:2489474/1‑149 (MQ=255)
                                                                                                                                   aaGCCGCTGCAGCAAAAGCGGCCGCAGAGGCAGATGATATTTTCGGTGAGCTAAGCTCTGGTAAGAATGCACCGAAAACGGGGGGAGGGGCGAAAGGGAACAATGCTTCGCCTGCCGGGAGTGGTAATACTAAAAACAATGGCGCATCa                  >  1:2941124/1‑149 (MQ=255)
                                                                                                                                   aaGCCGCTGCAGCAAAAGCGGCCGCAGAGGCAGATGATATTTTCGGTGAGCTAAGCTCTGGTAAGAATGCACCGAAAACGGGGGGAGGGGCGAAAGGGAACAATGCTTCGCCTGCCGGGAGTGGTAATACTAAAAACAATGGCGCATCa                  >  1:39191/1‑149 (MQ=255)
                                                                                                                                      ccGCTGCAGAAAACGCGGCCGCAGAGGCAGATGATATTTTCGGTGAGCTAAGCTCTGGTAAGAATGCACCGAAAACGGGGGGAGGGGCGAAAGGGAACAATGCTTCGCCTGCCGGGAGTGGTAATACTAAAAACAATGGCGCATCAggg               <  2:1997549/149‑1 (MQ=255)
                                                                                                                                             agaaacaGCGGCCGCAGAGGCTGATGATATTTTCGGTGAGCTAAGCTCTGGTAAGAATGCACCGAAAACGGGGGGAGGGGCGAAAGGGAACAATGCTTCGCCTGCCGGGAGTGGTAATACTAAAAACAATGGCGCATCAGGGGCCGata        <  2:579747/143‑1 (MQ=255)
                                                                                                                                              gcaAAAGCGGCCGCAGAGGCAGATGATATTTTCGGTGAGCTAAGCTCTGGTAAGAATGCACCGAAAACGGGGGGAGGGGCGAAAGGGAACAATGCTTCGCCTGCCGGGAGTGGTAATACTAAAAACAATGGCGCATCAGGGGCCGatat       >  1:2048999/1‑149 (MQ=255)
                                                                                                                                              gcaAAAGCGGCCGCAGAGGCAGATGATATTTTCGGTGAGCTAAGCTCTGGTAAGAATGCACCGAAAACGGGGGGAGGGGCGAAAGGGAACAATGCTTCGCCTGCCGGGAGTGGTAATACTAAAAACAATGGCGCATCAGGGGCCGatat       <  2:2945261/149‑1 (MQ=255)
                                                                                                                                                aataGCGGCCGCAGAGGCAGATGATATTTTCGGTGAGCTAAGCTCTGGTAAGAATGCACCGAAAACGGGGGGAGGGGCGAAAGGGAACAATGCTTCGCCTGCCGGGAGTGGTAATACTAAAAACAATGGCGCATCAGGGGCCGATATCa     >  1:2822297/4‑149 (MQ=255)
                                                                                                                                                 aaaGCGGCCGCAGAGGCAGATGATATTTTCGGTGAGCTAAGCTCTGGTAAGAATGCACCGAAAACGGGGGGAGGGGCGAAAGGGAACAATGCTTCGCCTGCCGGGAGTGGTAATACTAAAAACAATGGCGCATCAGGGGCCGATATCaa    >  1:1360097/1‑149 (MQ=255)
                                                                                                                                                  caGCGGCCGCAGAGGCAGATGATATTTTCGGTGAGCTAAGCTCTGGTAAGAATGCACCGAAAACGGGGGGAGGGGCGAAAGGGAACAATGCTTCGCCTGCCGGGAGTGGTAATACTAAAAACAATGGCGCATCAGGGGCCGATATCAAt   <  2:1115364/148‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                    
AGAGAAAGCTGCAGCCGACAAAAAAGCAGCAGAAAAAGCGGCTGCTGAAAAGGCAGCAGCTGATAAGAAAGCAGCGGCAGAAAAAGCCGCCGCAGACAAAAAAGCGGCAGCGGCAAAAGCTGCAGCTGAAAAAGCCGCTGCAGCAAAAGCGGCCGCAGAGGCAGATGATATTTTCGGTGAGCTAAGCTCTGGTAAGAATGCACCGAAAACGGGGGGAGGGGCGAAAGGGAACAATGCTTCGCCTGCCGGGAGTGGTAATACTAAAAACAATGGCGCATCAGGGGCCGATATCAATA  >  CP009273/772517‑772812

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑