Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 816,078 C→G 6.2% intergenic (+34/‑171) ybhL → / → ybhM UPF0005 family inner membrane protein/UPF0005 family inner membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273816,0780CG 6.2% 731.9 / 2.7 244intergenic (+34/‑171)ybhL/ybhMUPF0005 family inner membrane protein/UPF0005 family inner membrane protein
Reads supporting (aligned to +/- strand):  ref base C (107/115);  new base G (2/18);  total (111/133)
Fisher's exact test for biased strand distribution p-value = 7.62e-04
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 244 total aligned reads displayed.

ACCCGCGACACGTCGAACCTGCGCAAATATTCCATTCTTGGCGCGTTAACCTTGTATCTGGACTTCATCAACCTGTTCCTGATGTTGTTGCGGATCTTCGGCAACCGCCGTTAATTCTTTTTCACCATCACTATCCCGGCCATGTGTCGGGATTTTTTTCTTACTAAAAACGACACTTAACAAGTACCAGGTATAAAATATGCCTTATGGACGAGCGGAAGCGTTTTCTGTATCCATAAATGCAAAAGTATTTTGTATGCGTGGTTTTAAATTTATTGATGGTGAATATTAATAT  >  CP009273/815931‑816225
                                                                                                                                                   |                                                                                                                                                   
aCCCGCGACACGTCGAACCTGCGCAAATATTCCATTCTTGGCGCGTTAACCTTGTATCTGGACTTCATCAACCTGTTCCTGATGTTGTTGCGGATCTTCGGCAACCGCCGTTAATTCTTTTTCACCATCACTATCCCGGCCATGTGTCg                                                                                                                                                    >  2:5142656/1‑149 (MQ=255)
 cccGCGACACGTCGAACCTGCGCAAATATTCCATTCTTGGCGCGTTAACCTTGTATCTGGACTTCATCAACCTGTTCCTGATGTTGTTGCGGATCTTCGGCAACCGCCGTTAATTCTTTTTCACCATCACTATCCCGGCCATGTGTCgg                                                                                                                                                   >  2:3624932/1‑149 (MQ=255)
    gcgACACGTCGAACCTGCGCAAATATTCCATTCTTGGCGCGTTAACCTTGTATCTGGACTTCATCAACCTGTTCCTGATGTTGTTGCGGATCTTCGGCAACCGCCGTTAATTCTTTTTCACCATCACTATCCCGGCCATGTGTCGGGAt                                                                                                                                                <  1:2698074/149‑1 (MQ=255)
       acacGTCGAACCTGCGCAAATATTCCATTCTTGGCGCGTTAACCTTGTATCTGGACTTCATCAACCTGTTCCTGATGTTGTTGCGGATCTTCGGCAACCGCCGTTAATTCTTTTTCACCATCACTATCCCGGCCCTGTGTCGGGGtttt                                                                                                                                             >  1:2321976/1‑149 (MQ=255)
       acacGTCGAACCTGCGCAAATATTCCATTCTTGGCGCGTTAACCTTGTATCTGGACTTCATCAACCTGTTCCTGATGTTGTTGCGGATCTTCGGCAACCGCCGTTAATTCTTTTTCACCATCACTATCCCGGCCATGTGTCGGGAtttt                                                                                                                                             >  2:822736/1‑149 (MQ=255)
       acacGTCGAACCTGCGCAAATATTCCATTCTTGGCGCGTTAACCTTGTATCTGGACTTCATCAACCTGTTCCTGATGTTGTTGCGGATCTTCGGCAACCGCCGTTAATTCTTTTTCACCATCACTATCCCGGCCATGTGTCGGGAtttt                                                                                                                                             <  1:2979380/149‑1 (MQ=255)
       acacGTCGAACCTGCGCAAATATTCCATTCTTGGCGCGTTAACCTTGTATCTGGACTTCATCAACCTGTTCCTGATGTTGTTGCGGATCTTCGGCAACCGCCGTTAATTCTTTTTCACCATCACAATCCCGGCCATGTGTCGGGAtttt                                                                                                                                             >  1:4440131/1‑149 (MQ=255)
             cGAACCTGCGCAAATATTCCATTCTTGGCGCGTTAACCTTGTATCTGGACTTCATCAACCTGTTCCTGATGTTGTTGCGGATCTTCGGCAACCGCCGTTAATTCTTTTTCACCATCACTATCCCGGCCATGTGTCGGGAttttttttct                                                                                                                                       <  1:1321660/149‑4 (MQ=255)
               aaCCTGCGCAAATATTCCATTCTTGGCGCGTTAACCTTGTATCTGGACTTCATCAACCTGTTCCTGATGTTGTTGCGGATCTTCGGCAACCGCCGTTAATTCTTTTTCACCATCACTATCCCGGCCATGTGTCGGGATTTTTTTCTTAc                                                                                                                                     >  1:342708/1‑149 (MQ=255)
                aCCTGCGCAAATATTCCATTCTTGGCGCGTTAACCTTGTATCTGGACTTCATCAACCTGTTCCTGATGTTGTTGCGGATCTTCGGCAACCGCCGTTAATTCTTTTTCACCATCACTATCCCGGCCATGTGTCGGGATTTTTTTCTTACt                                                                                                                                    >  2:1003627/1‑149 (MQ=255)
                   tGCGCAAATATTCCATTCTTGGCGCGTTAACCTTGTATCTGGACTTCATCAACCTGTTCCTGATGTTGTTGCGGATCTTCGGCAACCGCCGTTAATTCTTTTTCACCATCACTATCCCGGCCATGTGTCGGGATTTTTTTCTTACTaaa                                                                                                                                 >  1:4273389/1‑149 (MQ=255)
                      gcAAATATTCCATTCTTGGCGCGTTAACCTTGTATCTGGACTTCATCAACCTGTTCCTGATGTTGTTGCGGATCTTCGGCAACCGCCGTTAATTCTTTTTCACCATCACTATCCCGGCCATGTGTCGGGATTTTTTTCTTACTAAAAAc                                                                                                                              <  2:2226151/149‑1 (MQ=255)
                      gcAAATATTCCATTCTTGGCGCGTTAACCTTGTATCTGGACTTCATCAACCTGTTCCTGATGTTGTTGCGGATCTTCGGCAACCGCCGTTAATTCTTTTTCACCATCACTATCCCGGCCATGTGTCGGGATTTTTTTCTTACTAAAAAc                                                                                                                              >  2:122114/1‑149 (MQ=255)
                       cAAATATTCCATTCTTGGCGCGTTAACCTTGTATCTGGACTTCATCAACCTGTTCCTGATGTTGTTGCGGATCTTCGGCAACCGCCGTGAATTCTTTTTCACCATCACAATCCCGGCCAAGTTTCGGGCTTTTTTTATTACTAAAAAcc                                                                                                                             >  1:41486/1‑148 (MQ=255)
                        aaaTCTTCCATTCTTGGCGCGTTAAACTTTTATCTGGACTTCATCAACCTTTTCCTTATGTTGTTGCGGATCTTCGGAAACCGCCTTTAATTCTTTTTCACCATCACTATCCCGGCAATGTGTCGGGATTTTTTTATTACTAAAAACGa                                                                                                                            <  2:3074577/149‑1 (MQ=255)
                        aaaTATTCCATTCTTGGCGCGTTAACCTTGTATCTGGACTTCATCACCCTGTTCCTGATGTTGTTGCGGATCTTCGGCAACCGCCGTTAATTCTTTTTCACCATCACTATCCCGGCCATGTGTCGGGATTTTTTTCTTACTAAAAACGa                                                                                                                            <  2:4282366/149‑1 (MQ=255)
                        aaaTATTCCATTCTTGGCGCGTTAACCTTGTATCTGGACTTCATCAACCTGTTCCTGATGTTGTTGCGGATCTTCGGCAACCGCCGTTAATTCTTTTTCACCATCACTATCCCGGCCATGTGTCGGGATTTTTTTCTTACTAAAAACGa                                                                                                                            >  2:4624827/1‑149 (MQ=255)
                        aaaTATTCCATTCTTGGCGCGTTAACCTTGTATCTGGACTTCATCAACCTGTTCCTGATGTTGTTGCGGATCTTCGGCAACCGCCGTTAATTATTTTTCACCATCACTATCCCGGCCATGTGTGGGGATTTTTTTCTTACTAAAAACGa                                                                                                                            <  1:4771289/149‑1 (MQ=255)
                         aaTATTCCATTCTTGGCGCGTTAACCTTGTATCTGGACTTCATCAACCTGTTCCTGATGTTGTTGCGGATCTTCGGCAACCGCCGTTAATTCTTTTTCACCATCACTATCCCGGCCATGTGTCGGGATTTTTTTCTTACTAAAAAAGac                                                                                                                           >  1:4925128/1‑149 (MQ=255)
                         aaTATTCCATTCTTGGCGCGTTAACCTTGTATCTGGACTTCATCAACCTGTTCCTGATGTTGTTGCGGATCTTCGGCAACCGCCGTTAATTCTTTTTCACCATCACTATCCCGGCCATGTGTCGGGATTTTTTTCTTAATAAAAAAGac                                                                                                                           >  1:1249893/1‑149 (MQ=255)
                            atTCCATTGTTGGCGCTTTAACCTTTTATCTGGATTTAATCAACTTGTTCCTTTTTTTGTTGCGTATTTTCGGAAACCGCCTTTATTTCTTTTTCACAATCACTATCCGGGCAAGGTGTCGGTATTTTTTTCTTACTAAAAACGACACt                                                                                                                        <  2:1835985/149‑1 (MQ=255)
                                 aTTCTTGGCGCGTTAACCTTGTATCTGGACTTCATCAACCTGTTCCTGATGTTGTTGCGGATCTTCGGCAACCGCCGTTAATTCTTTTTCACCATCACTATCCCGGCCATGTGTCGGGATTTTTTTCTTACTAAAAACGACACTTAACa                                                                                                                   >  1:1947447/1‑149 (MQ=255)
                                 aTTCTTGGCGCGTTAACCTTGTATCTGGACTTCATCAACCTGTTCCTGATGTTGTTGCGGATCTTCGGCAACCGCCGTTAATTCTTTTTCACCATCACAATACCGGCCAAGTGGCGGGATTTTTTTCTTACTAAAAACGACCCATAACa                                                                                                                   >  1:3104315/1‑149 (MQ=255)
                                   tCTTGGCGCGTTAACCTTGTATCTGGACTTCATCAACCTGTTCCTGATGTTGTTGCGGATCTTCGGCAACCGCCGTTAATTCTTTTTCACCATCACTATCCCGGCCTTGTGTCGGGATTTTTTTCTTACTAAAAACGACACTTAACAAg                                                                                                                 <  2:2800872/149‑1 (MQ=255)
                                     ttGGCGCGTTAACCTTGTATCTGGACTTCATCAACCTGTTCCTTTTGTTGTTGCGTATATTCGGAAACCGCCTTTAATTCTTTTTCACCATCACTATCCCGGCCATGTGTCGGTATTTTTTTCTTACTAAAAACGACACTTAACAAGTa                                                                                                               <  2:747371/149‑1 (MQ=255)
                                     ttGGCGCGTTAACCTTGTATCTGGACTTCATCAACCTGTTCCTGATGTTGTTGCGGATCTTCGGCAACCGCCGTTAATTCTTTTTCACCATCACTATCCCGGCCAGGTGTCGGGATTTTTTTCTTACTAAAAACGACACTTAACAAGTa                                                                                                               >  2:4066474/1‑149 (MQ=255)
                                      gggCGCGTTAACGTTTTATCTGGACTTCATCAACGTGTTCCTTATGTTGTTGCGTATCTTCGGAAACCGCCTTTAATTCTTTTTCACCATCACTATCCCGGCCATGTGTCGGTATTTTTTTCTTACTAAAAACGACACTTAACAAGTAc                                                                                                              <  2:4850858/148‑1 (MQ=255)
                                       ggCGCGTAAACCTTGTATCTGGACTTCATCAACCTGTTCCTGATGTGGTTGCGGATCTTCGGCAACCGCCGTTAATTATTTTTCACCATCACTATCCCGGCCTTGTGTCGGGTTTTTTTTCTTACTAAAAACGACACTTAACAAGTAcc                                                                                                             <  1:5181082/149‑1 (MQ=255)
                                        gcgcGTTAACCTTGTATCTGGACTTCATCAACCTGTTCGTTTTGTTGTTGCGGATCTTGGGCAACCGCCTTTATTTCTTTTTCACCATCACTATCCCGGCCTTGTGTCGGTTTTTTTTTCTTACTAAAAACGACACTTAACAAGTACCa                                                                                                            <  1:2042330/149‑1 (MQ=255)
                                          gctttAACCTTTTATGTGGACTTAAACAACCTGTTCTTGTTGTTGTTGCGTATCTTCGGCAACCGCCTTTATTTCTTTTTCACCAACACTACCCCGGCCTTGTGTCGGTATTTTTTTTTTAATAAAAACGACACTTAACAAGTACCAgg                                                                                                          <  1:207700/146‑1 (MQ=255)
                                          gcgTTAACCTTGTATCTGGACTTCATCAACCTGTTCCTGATGTTGTTGCGGATCTTCGGCAACCGCCGTTAATTCTTTTTCACCATCACTATCCCGGCCATGTGTCGGGATTTTTTTCTTACTAAAAACGACACTTAACAAGTACCAgg                                                                                                          >  1:1460685/1‑149 (MQ=255)
                                          gcgTTAACCTTGTATCTGGACTTCATCAACCTGTTCCTGATGTTGTTGCGGATCTTCGGCAACCGCCGTTAATTCTTTTTCACCATAACAATCCCGGCCATGTGTCGGGATTTTTTTCTTAATAAAAACGACACTTAACAAGTACCAgg                                                                                                          >  1:3301851/1‑149 (MQ=255)
                                            tttAACCTTTTATCTGGACTTCATAAACCTGTTCCTGATGTTGTTGCGTATCTTCGGCAACCGCCTTTAATTCTTTTTCACCAACACTATCCCGGCCATGTGTCGGTATTTTTTTCTTACTAAAAACGACACTTAACAAGTACCAGGta                                                                                                        <  2:5004465/148‑1 (MQ=255)
                                             ttAACCTTGTATCTGGACTTCATCAACCTGTTCCTGATGTTGTTGCGGATCTTCGGCAACCGCCGTTAATTCTTTTTCACCATCACTATACCGGCCATTTGTAGGGATTTTTTTCTTACTAAAAAAGACACTTAACAATTACCAGGtat                                                                                                       >  1:1987211/1‑149 (MQ=255)
                                              tAACCTTGTATCTGGACTTCATCAACCTGTTCCTGATGTTGTTGCGGATCTTCGGCAACCGCCGTTAATTCTTTTTCACCATCACTATCCCGGCCATGTGTCGGGATTTTTTTCTTACTAAAAACGACACTTAACAAGTACCAGGtata                                                                                                      >  1:1618706/1‑149 (MQ=255)
                                                aCCTTGTATCTGGACTTCATCAACCTGTTCCTGATGTTGTTGCGGATCTTCGGCAACCGCCGTTAATTAATTTTAACCATCACTATCCCCGCCATTTTTAGGGATTTTTTTAATACTAAAAAAGAAACTTAAAAATTAAAAGGTAaaaa                                                                                                    >  1:3640831/1‑149 (MQ=255)
                                                 ctttttATCTGGACTACATCACCCTGTTCCTGTTTTTGTGGCGTATCTGCGGCAACCGCCTTAAATTCTTTTCCACCAACACTATCCCGGCCTTGTGTGGTTTTTTTTTTATTAAAAAAAACGACACTTAACAAGTACCAGGTATaaaa                                                                                                   <  1:4031178/144‑1 (MQ=255)
                                                 cctggcaTCTGGACTTCAACAACCTGTACCGGTTGTTGTTGCGGTTTTTCGGAAACCCCCTTTATTTATTTTACACCATCACTATCCCGGCCTTGTGTGGGTTTTTTTTTCTTACTAACAACGACACTTAACAAGTACCAGGTATaaaa                                                                                                   <  1:1410519/143‑1 (MQ=255)
                                                     gTATCTGGACTTCATCAACCTGTTCCTGATGTTGTTGCGGATCTTCGGCAACCGCCGTTATTTCTTTTTCACCATCACTATCCCGGCCATGTGTCGGGTTTTTTTTCTTACTAAAAACGACACTTAACAAGTACCAGGTATAAAATATg                                                                                               <  2:3452067/149‑1 (MQ=255)
                                                       atgtGGACTTCAACAACCTGTTCTTGTTTTTGTTGCGGATCTTCGGAAACCGCCGTTATTTCTTTTTCACAATCACTATCCCGGCATTGTGTGGGTATTTTTTTCTTAATAAAAACGACACTTAACAAGTACCAGGTATAAAATATGcc                                                                                             <  1:235970/146‑1 (MQ=255)
                                                        tCTGGACTTCATCAACCTGTTCCTGATGTTGTTGCGTATCTTCGGCAACCGCCTTTAATTATTTTTCACCATCACTATCCCGGCCATGTGTCGGTATTTTTTTCTTACTAAAAACGACACTTAACAAGTACCAGGTATAAAATATGCCt                                                                                            <  2:2462327/149‑1 (MQ=255)
                                                        tCTGGACTTCATCAACCTGTTCCTGATGTTGTTGCGGATCTTCGGCAACCGCCGTTAATTCTTTTTCACCATCACTATCCCGGCCATGTGTCGGGATTTTTTTCTTACTAAAAACGACACTTAACAAGTACCAGGTATAAAATATGCCt                                                                                            <  2:299611/149‑1 (MQ=255)
                                                        tCTGCACTTCATCAACCTGTTCCTGATGTTGTTGCGTATCTTCGGCAACCGCCTTTAATTCTTTTTCACCATCCCTAGCCCGGCCTTGTGTCGGGATTTTTTTCTTACTAAAAACGACACTTAACAAGTACCAGGTATAAAATATGCCt                                                                                            <  2:3552839/149‑1 (MQ=255)
                                                         cTGGACTTCATCAACCTGTTCCTGATGTTGTTGCGGATCTTCGGCAACCGCCGTTAATTCTTTTTCACCATCACTATCCCGGCCATGTGTCGGGATTTTTTTCTTACTAAAAACGACACTTAACAAGTACCAGGTATAAAATATGCCtt                                                                                           >  2:140936/1‑149 (MQ=255)
                                                          tGGACTTCAAAAACCTGTTCGTGTTGTTGTTGGGTATCTTCGGCACCCGCCTTTAATTCTTTTTCACCATCACTATCCCGGCCTGGTGTCGGGATTTTTTTCTTACTAAAAACGACACTTAACAAGTACCAGGTATAAAATATGCCTTa                                                                                          <  2:2189893/149‑1 (MQ=255)
                                                           ggACTTCATCAACCTGTTCCTGATGTTGTTGCGGATCTTCGGCAACCGCCGTTAATTCTTTTTCACCATCACTATCCCGGCCATGTGTCGGTATTTTTTTCTTACTAAAAACGACACTTAACAAGTACCAGGTATAAAATATGCCTTAt                                                                                         <  2:375137/149‑1 (MQ=255)
                                                            gACTTCATCAACCTGTTCCTGATGTTGTTGCGGATCTTCGGCAACCGCCGTTAATTATTTTTCACCATCACTATCCCGGCCATGTGTCGGGATTTTTTTCTTACTAAAAACGACACTTAACAAGTACCAGGTATAAAATATGCCATATg                                                                                        >  2:4807041/1‑149 (MQ=255)
                                                             aCTTCATCAACCTGTTCGTTATGTTGTTGCGTATCTTCGGAAACCGCCGTTAATTCTTTTTCACCATCACTATCCCGGCATTGTGTCGGTATTTTTTTCTTACTAAAAACGACACTTAACAAGTACCAGGTATAAAATATGCCTTATgg                                                                                       <  1:5080084/149‑1 (MQ=255)
                                                             aCTTCATCAACCTGTTCCTGATGTTGTTGCGGATCTTCGGCAACCGCCGTTAATTCTTTTTCACCATCACTATCCCGGCCATGTGTCGGGATTTTTTTCTTACTAAAAACGACACTTAACAAGTACCAGGTATAAAATATGCCTTATgg                                                                                       >  1:2148281/1‑149 (MQ=255)
                                                                  atcaACCTGTTCCTGATGTTGTTGCGGATCTTCGGCAACCGCCGTTAATTCTTTTTCACCATCACTATCCCGGCCAGGTGTCGGGATTTTTTTCTTACTAAAAACGACACTTAACAAGTACCAGGTATAAAATATGCCTTATGGACGAg                                                                                  <  2:370328/149‑1 (MQ=255)
                                                                    caACCTGTTCATTATGTTGTTGCGTATGTTCGGAAACCGCCTTTAATTCTTTTTACCCATCACTATCCCGGCCATGTTTGGGGATTTTTTTATTAATAAAAACGACACTTAACAAGTACCAGGTATAAAATATGCCTTATGGACGAGCg                                                                                <  2:3465668/149‑1 (MQ=255)
                                                                       ccTGTTCCTGATGTTGTTGCGGATCTTCGGCAACCGCCGTTAATTCTTTTTCACCATCACTATCCCGGCCATGTGTCGGGATTTTTTTCTTACTAAAAACGACACTTAACAAGTACCAGGTATAAAATATGCCTTATGGACGAGCGGaa                                                                             >  2:378929/1‑149 (MQ=255)
                                                                        cTGTTCCTGATGTTGTTGCGGATCTTCGGCAACCGCCGTTAATTCTTTTTCACCATCACTAGCCCGGCCATGTGTCGGGATTTTTTTCTTACTAAAAACGACACTTAACAAGTACCAGGTATAAAATATGCCTTATGGACGAGCGGAAg                                                                            <  2:3203169/149‑1 (MQ=255)
                                                                              cTGATGTTGTTGCGGATCTTCGGCAACCGCCGTTAATTCTTTTTCACCATCACTATCCCGGCCATGTGTCGGGATTTTTTTCTTACTAAAAACGACACTTAACAAGTACCAGGTATAAAATATGCCTTATGGACGAGCGGAAGCGtttt                                                                      >  1:2076994/1‑149 (MQ=255)
                                                                                gATGTTGTTGCGGATCTTCGGCAACCGCCGTTAATTCTTTTTCACCATCACTATCCCGGCCATGTGTCGGGATTTTTTTATTACTAAAAACGACACTTAACAAGTACCAGGTATAAAATATTCCTTATGGACGAGCGGAAGCGTTTTCt                                                                    >  1:1232548/1‑149 (MQ=255)
                                                                                  tgttgtTGCGTATCTTCGGCAACCGCCGTTAATTCTTTTTCACCATCACTATCCCGGCCATGTGTCGGTATTTTTTTCTTACTAAACACGACACTTAACAAGTACCAGGTATAAAATATGCCTTATGGACGAGCGGAAGCGTTTTCTGt                                                                  <  2:544295/149‑1 (MQ=255)
                                                                                  tgttgtTGCGGATCTTCGCCAACCGCCGTTAATTCTTTTACACCATCACTATCCCGGCCATGTGTCGGTATTTTTTTCTTACTAAAAACGACACTTAACAAGTACCAGGTATAAAATATGCCTTATGGACGAGCGGAAGCGTTTTCTGt                                                                  <  2:1115831/149‑1 (MQ=255)
                                                                                   gttgttGCGGATCTTCGGCAACCGCCGTTAATTCTTTTTCACCATCACTATCCCGGCCATGTGTCGGGATTTTTTTCTTACTAAAAACGACACTTAACAAGTACCAGGTATAAAATATGCCTTATGGACGAGCGGAAGCGTTTTCTGTa                                                                 <  2:2959756/149‑1 (MQ=255)
                                                                                   gttgttGCGGATCTTCGGCAACCGCCGTTAATTCTTTTTCACCATCACTAGCCCGGCCATGTGTCGGTATTTTTTTCTTACAAAAAACGACACTTAACAAGTACCAGGTATAAAATATGCCTTATGGACGAGCGGAAGCGTTTTCTGTa                                                                 <  2:4489320/149‑1 (MQ=255)
                                                                                   gttggtGCGGATCTTCGGCAACCGCCGTTAATTCTTTTTCACCATCACTCTCCCGGCCAGGTGTCGGGATTTTTTACTTACTAAAAACGACACTTAACAAGTACCAGGTATAAAATATGCCTTATGGACGAGCGGAAGCGTTTTCTGTa                                                                 <  2:4488847/149‑1 (MQ=255)
                                                                                       ttgCGGATCTGCGGCAACCGCCGTTAATTCTTTTACACCATCACTATCCCGGCCATGTGTCGGGATTTTTTTCTTACTAAACACGACACTTAACAAGTACCAGGTATAAAATATGCCTTATGGACGAGCGGAAGCGTTTTCTGTATCCa                                                             <  2:3541577/149‑1 (MQ=255)
                                                                                         gCGGATCTTCGGCAACCGCCGTTAATTCTTTTTCACCATCACTATCCCGGCCATGTGTCGGGATTTTTTTCTTCCTAAAAACGACACTTAACAAGTACCAGGTATAAAATATGCCTTATGGACGAGCGGAAGCGTTTTCTGTATCCATa                                                           <  2:4251670/149‑1 (MQ=255)
                                                                                           ggATCTTCGGCAACCGCCGTTAATTCTTTTTCACCATCACTATCCCGGCCATGTGTCGGGATTTTTTTCTTACTAAAAACGACACTTAACAAGTACCAGGTATAAAATATGCCTTATGGACGAGCGGAAGCGTTTTCTGTATCCATaaa                                                         <  2:3849072/149‑1 (MQ=255)
                                                                                            gATGTTCGGAAACCGCCTTTAATTATTTTTCACCATCACTATCCCGGCCATGTGTCGGGATTTTTTTATTAAAAAAAACGACACTTAACAAGTACCAGGTATAAAATATGCCTTATGGACGAGCGGAAGCGTTTTCTGTATCCATAAAt                                                        <  1:4478516/149‑1 (MQ=255)
                                                                                            gATCTTCGGCAACCGCCTTTAATTCTTTTTCACCATCACTATCCCGGCCTTGTGTCGGTTTTTTTTTCTTACTAAAAACGACACTTAACAAGTACCAGGTATAAAATATGCCTTATGGACGAGCGGAAGCGTTTTCTGTATCCATAAAt                                                        <  1:1870601/149‑1 (MQ=255)
                                                                                             aTCTTCGGCAACCGCCGTTAATTCTTTTTCACCATCACTATCCCGGCCATGTGTCGGGATTTTTTTCTTACAAAAAACGACCCTTAACAAGTACCAGGTATAAAATATGCCTTATGGACGAGCGGAAGCGTTTTCTGTATCCATAAATg                                                       <  2:2487279/149‑1 (MQ=255)
                                                                                              tCTTCGGCAACCGCCGTTAATTCTTTTTCACCATCACTATCCCGGCCATGTGTCGGGATTTTTTTCTTACTAAAAACGACACTTAACAAGTACCAGGTATAAAATATGCCTTATGGACGAGCGGAAGCGTTTTCTGTATCCATAAATGc                                                      <  1:820806/149‑1 (MQ=255)
                                                                                              tCTTCGGCAACCGCCGTTAATTCTTTTACACCATCACTATCCCGGCCCTGTGTCGGTATTTTTTTCTTACTAAAAACGACACTTAACAAGTACCAGGTATAAAATATGCCTTATGGACGAGCGGAAGCGTTTTCTGTATCCATAAATGc                                                      <  1:4722779/149‑1 (MQ=255)
                                                                                               cTTCGGCAACCGCCGTTAATTCTTTTTCACCATCACTATCCCGGCCATGTGTCGGGATTTTTTTCTTACTAAAAACGACACTTAACAAGTACCAGGTATAAAATATGCCTTATGGACGATCGGAAGCGTTTTATGTATCCATAAATGCa                                                     >  2:3284366/1‑149 (MQ=255)
                                                                                                  cGGCAACCGCGTTTATTTCTTTTACACCAACAACACCCCGCCCTTGTGTGGTTTTTTTTTTCTTACAAAAAACGACACTTAACAAGTACCAGGTATAAAATATGCCTTATGGACGAGCGGAAGCGTTTTCTGTATCCATAAATGCaaaa                                                  <  1:462261/149‑1 (MQ=255)
                                                                                                  cGGAAACCGCCGTTAATTCTTTTTCACAATCACTATCCCGGCCTTGTGTCGGGATTTTTTTCTTACTAAACACGACACTTAACAAGTACCAGGTATAAAATATGCCTTATGGACGAGCGGAAGCGTTTTCTGTATCCATAAATGCaaaa                                                  <  1:4193661/149‑1 (MQ=255)
                                                                                                    gCAACCGCCGTTAATTCTTTTTCACCATCACTATCCCGGCCATGTGTCGGGATTTTTTTCTTACTAAAAACGACACTTAACAAGTACCAGGTATAAAATATGCCTTATGGACGAGCGGAAGCGTTTTCTGTATCCATAAATGCAAAAGt                                                <  2:2422986/149‑1 (MQ=255)
                                                                                                    gCAACCGCCGTTAATTATTTTTCACCATCAATATCCCGGCAATGTGTCGGTTTTTTTTTATTACTAAAAACGACACTTAACAAGTACCAGGTATAAAATATGCCTTATGGACGAGCGGAAGCGTTTTCTGTATCCATAAATGCAAAAGt                                                <  1:3874912/149‑1 (MQ=255)
                                                                                                        ccgcctTTAATTCTTTTACACCATCACTATCCCGGCCCTGTGTCGGTATTTTTTTCTTACTAAAAACGACACTTAACAAGTACCAGGTATAAAATATGCCTTATGGACGAGCGGAAGCGTTTTCTGTATCCATAAATGCAAAAGTAttt                                            <  1:2793685/149‑1 (MQ=255)
                                                                                                          gccgTTAATTCTTTTTCACCATCACTCTCCCGGCCAGGGGTCGGGATTTTTTTCTTACTAAAAACGACACTTAACAAGTACCAGGTATAAAATATGCCTTATGGACGAGCGGAAGCGTTTTCTGTATCCATAAATGCAAAAGTATTTTg                                          <  1:3814209/149‑1 (MQ=255)
                                                                                                            cgTTAATTCTTTTTCACCATCACTATCCCGGCCATGTGTCGGTATTTTTTTCTTACTAAAAACGACACTTAACAAGTACCAGGTATAAAATATGCCTTATGGACGAGCGGAAGCGTTTTCTGTATCCATAAATGCAAAAGTATTTTGTa                                        <  2:289333/149‑1 (MQ=255)
                                                                                                            cgTTAATTCTTTTTCACCATCACTATCCCGGCCATGTGTCGGGATTTTTTTCTTACTAAAAACGACACTTAACAAGTACCAGGTATAAAATATGCCTTATGGACGAGCGGAAGCGTTTTCTGTATCCATAAATGCAAAAGTATTTTGTa                                        >  2:320344/1‑149 (MQ=255)
                                                                                                             gtaaaTTCTTTTCCACCATCACTAGCCCGCCCATGTGTCGGTATTTTTTTCTTACAAAAAACGACACTTAACAAGTACCAGGTATAAAATATGCCTTATGGACGAGCGGAAGCGTTTTCTGTATCCATAAATGCAAAAGTATTTTGTAt                                       <  1:4496028/146‑1 (MQ=255)
                                                                                                              ttAATACTTTTACACCATCACTATCCCGGCCATGTGTCGGGATTTTTTTATAAAAAAAAACGACACTTAACAAGTACCAGGTATAAAATATGCCTTATGGACGAGCGGAAGCGTTTTCTGTATCCATAAATGCAAAAGTATTTTGTATg                                      <  1:4589785/149‑1 (MQ=255)
                                                                                                               tAATTGTTTTCCACCATCACTAGCCCGGCCTTGGGTCGGTTTTTTTTTCTTACTAAAAACGACACTTAACAAGTACCAGGTATAAAATATGCCTTATGGACGAGCGGAAGCGTTTTCTGTATCCATAAATGCAAAAGTATTTTGTATGc                                     <  1:3554995/149‑1 (MQ=255)
                                                                                                               tAATTCTTTTTCACCATCACTATCCCGGCCATGTGTCGGGATTTTTTTCTTACTAAAAACGACACTTAACAAGTACCAGGTATAAAATATGCCTTATGGACGAGCGGAAGCGTTTTCTGTATCCATAAATGCAAAAGTATTTTGTATGc                                     >  1:2081663/1‑149 (MQ=255)
                                                                                                                     tttttCACCATCACTATCCCGGCCATGTGTCGGGATTTTTTTCTTACTAAAAACGACACTTAACAAGTACCAGGTATAAAATATGCCTTATGGACGAGCGGAAGCGTTTTCTGTATCCATAAATGCAAAAGTATTTTGTATGCGTGGtt                               >  2:3249972/1‑149 (MQ=255)
                                                                                                                     tttttCACCATCACTATCCCGGCCATGTGTCGGGATTTTTTTCTTACTAAAAACGACACTTAACAAGTACCAGGTATAAAATATGCCTTATGGACGAGCGGAAGCGTTTTCTGTATCCATAAATGCAAAAGTATTTTGTATGCGTGGtt                               >  2:1571828/1‑149 (MQ=255)
                                                                                                                     ttttaCACCATCACTAGCCCGGCCTTGTGTCGGTATTTTTTTCTTACAAAAAACGACACTTAACAAGTACCAGGTATAAAATATGCCTTATGGACGAGCGGAAGCGTTTTCTGTATCCATAAATGCAAAAGTATTTTGTATGCGTGGtt                               <  1:981557/149‑1 (MQ=255)
                                                                                                                         tCACCATCACTATCCCGGCCAGGTGTCGGTATTTTTTTCTTACTAAAAACGACACTTAACAAGTACCAGGTATAAAATATGCCTTATGGACGAGCGGAAGCGTTTTCTGTATCCATAAATGCAAAAGTATTTTGTATGCGTGGTTTTaa                           <  2:2725410/149‑1 (MQ=255)
                                                                                                                          cACCATCACTCTCCCGGCCAGGTGTGGGTATTTTTTTCTTACAAAACACGACACTTAACAAGTACCAGGTATAAAATATGCCTTATGGACGAGCGGAAGCGTTTTCTGTATCCATAAATGCAAAAGTATTTTGTATGCGTGGTTTTaaa                          <  1:3178988/149‑1 (MQ=255)
                                                                                                                          cACCATCACTATCCCGGCCATGTGTCGGGATTTTTTTCTTACTAAAAACGACACTTAACAAGTACCAGGTATAAAATATGCCTTATGGACGAGCGGAAGCGTTTTCTGTATCCATAAATGCAAAAGTATTTTGTATGCGTGGTTTTaaa                          >  1:4264283/1‑149 (MQ=255)
                                                                                                                           cccATCACTATCCCGGCCATGTGTCGGGATTTTTTTCTTACTAAAAACGACACTTAACAAGTACCAGGTATAAAATATGCCTTATGGACGAGCGGAAGCGTTTTCTGTATCCATAAATGCAAAAGTATTTTGTATGCGTGGTTTTAAAt                         <  2:1579909/148‑1 (MQ=255)
                                                                                                                            ccATCACTATCCCGGCCATGTGTCGGTATTTTTTTCTTACTAAAAACGACACTTAACAAGTACCAGGTATAAAATATGCCTTATGGACGAGCGGAAGCGTTTTCTGTATCCATAAATGCAAAAGTATTTTGTATGCGTGGTTTTAAAtt                        <  2:428976/149‑1 (MQ=255)
                                                                                                                             cATCACTATCCCGGCCATGTGTCGGGATTTTTTTCTTACTAAAAACGACACTTAACAAGTACCAGGTATAAAATATGCCTTATGGACGAGCGGAAGCGTTTTCTGTATCCCTAAATGCAAAAGTATTTTGTATGCGGGGTTTTAAAttt                       >  1:2625665/1‑149 (MQ=255)
                                                                                                                             cATCACTATCCCGGCCATGTGTCGGGATTTTTTTCTTACTAAAAACGACACTTAACAAGTACCAGGTATAAAATATGCCTTATGGACGAGCGGAAGCGTTTTCTGTATCCATAAATGCAAAAGTATTTTGTATGCGTGGTTTTAAAttt                       >  1:4499092/1‑149 (MQ=255)
                                                                                                                              aTCACTATCCCGGCCATGTGTCGGGATTTTTTTCTTACTAAAAACGACACTTAACAAGTACCAGGTATAAAATATGCCTTATGGACGAGCGGAAGCGTTTTCTGTATCCATAAATGCAAAAGTATTTTGTATGCGTGGTTTTAAATTTa                      >  2:3769855/1‑149 (MQ=255)
                                                                                                                                cACTATCCCGGCCATGTGTCGGGATTTTTTTCTTACTAAAAACGACACTTAACAAGTACCAGGTATAAAATATGCCTTATGGACGAGCGGAAGCGTTTTCTGTATCCATAAATGCAAAAGTATTTTGTATGCGTGGTTTTAAATTTAtt                    >  1:4312967/1‑149 (MQ=255)
                                                                                                                                cACTATCCCGGCCATGGGTCGGTATTTTTTTCTTAAAAAAAACGACACTTAACAAGTACCAGGTATAAAATATGCCTTATGGACGAGCGGAAGCGTTTTCTGTATCCATAAATGCAAAAGTATTTTGTATGCGTGGTTTTAAATTTAtt                    <  1:3440220/149‑1 (MQ=255)
                                                                                                                                 aCTATCCCGGCCTTGTGTCGGGATTTTTTTCTTACTAAAAACGACACTTAACAAGTACCAGGTATAAAATATGCCTTATGGACGAGCGGAAGCGTTTTCTGTATCCATAAATGCAAAAGTATTTTGTATGCGTGGTTTTAAATTTATTg                   <  1:737597/149‑1 (MQ=255)
                                                                                                                                    aTCCCGGCCATGTGTCGGGATTTTTTTCTTACTAAAAACGACACTTAACAAGTACCAGGTATAAAATATGCCTTATGGACGAGCGGAAGCGTTTTCTGTATCCATAAATGCAAAAGTATTTTGTATGCGTGGTTTTAAATTTATTGATg                >  2:52706/1‑149 (MQ=255)
                                                                                                                                    aTCCCGGCCATGTGTCGGGATTTTTTTCTTACTAAAAACGACACTTAACAAGTACCAGGTATAAAATATGCCTTATGGACGAGCGGAAGCGTTTTCTGTATCCATAAATGCAAAAGTATTTTGTATGCGTGGTTTTAAATTTATTGATg                >  2:1787692/1‑149 (MQ=255)
                                                                                                                                          gCCATGTGTCGGGATTTTTTTCTTACTAAAAACGACACTTAACAAGTACCAGGTATAAAATATGCCTTATGGACGAGCGGAAGCGTTTTCTGTATCCATAAATGCAAAAGTATTTTGTATGCGTGGTTTTAAATTTATTGATGGTGaat          >  2:2632054/1‑149 (MQ=255)
                                                                                                                                                tgtCGGGATTTTTTACTTACAAACAACGACACTTAACAAGTACCAGGTATAAAATATGCCTTATGGACGAGCGGAAGCGTTTTCTGTATCCATAAATGCAAAAGTATTTTGTATGCGTGGTTTTAAATTTATTGATGGTGaatattaat    <  2:3100915/149‑1 (MQ=255)
                                                                                                                                                  tCGGGATTTTTTTCTTACTAAAAACGACACTTAACAAGTACCAGGTATAAAATATGCCTTATGGACGAGCGGAAGCGTTTTCTGTATCCATAAATGCAAAAGTATTTTGTATGCGTGGTTTTAAATTTATTGATGGTGaatattaatat  >  2:2833743/1‑149 (MQ=255)
                                                                                                                                                   |                                                                                                                                                   
ACCCGCGACACGTCGAACCTGCGCAAATATTCCATTCTTGGCGCGTTAACCTTGTATCTGGACTTCATCAACCTGTTCCTGATGTTGTTGCGGATCTTCGGCAACCGCCGTTAATTCTTTTTCACCATCACTATCCCGGCCATGTGTCGGGATTTTTTTCTTACTAAAAACGACACTTAACAAGTACCAGGTATAAAATATGCCTTATGGACGAGCGGAAGCGTTTTCTGTATCCATAAATGCAAAAGTATTTTGTATGCGTGGTTTTAAATTTATTGATGGTGAATATTAATAT  >  CP009273/815931‑816225

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑