Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 847,954 A→C 5.2% L34V (TTA→GTA)  ybiP ← putative EptAB family phosphoethanolamine transferase, inner membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273847,9540AC 5.2% 1032.0 / 2.4 330L34V (TTA→GTA) ybiPputative EptAB family phosphoethanolamine transferase, inner membrane protein
Reads supporting (aligned to +/- strand):  ref base A (188/110);  new base C (2/27);  total (191/139)
Fisher's exact test for biased strand distribution p-value = 2.06e-09
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 336 total aligned reads displayed.

CCGTAGGCCTGAGCAAAAGGGAAATAACAAGCCGCCACCAGCGAACTGACACCGACCAGAACTTTTTGTACGCGAGGCAATGTTCGCC‑ATAGCAAAAGCAAAA‑TAGCCGTAAA‑CGCAGCGGTGTAGAGCAAACTGAAGGGGTAACCTAAGCCGAGGTTAATTAATAGCGACTGTAAAAAGTAGAACGCAGTCCACGGGCTAAATACCCGGCTACGGGTAACAAGCGATTCTTTGAGGGTTAAATTCATATGCCACTATCGTAACAAACGCCATGTGCTTACCCTGGCGTCAAGGGT  >  CP009273/847806‑848101
                                                                                                                                                       |                                                                                                                                                   
  gTAGGCCTGAGCAAAAGGGAAATAACAAGCCGCCACCAGCGAACTGACACCGACCAGAACTTTTTGTACGCGAGGCAATGTTCGCC‑ATAGCAAAAGCAAAA‑TAGCCGTAAA‑CGCAGCGGTGTAGAGCAAACTGAAGGGGTAACCTAAGc                                                                                                                                                   <  2:2298203/149‑1 (MQ=255)
  gTAGGCCTGAGCAAAAGGGAAATAACAAGCCGCCACCAGCGAACTGACACCGACCAGAACTTTTTGTACGCGAGGCAATGTTCGCC‑ATAGCAAAAGCAAAA‑TAGCCGTAAA‑CGCAGCGGTGTAGAGCAAACTGAAGGGGTAACCTAAGc                                                                                                                                                   >  2:662079/1‑149 (MQ=255)
    aGGCCTGAGCAAAAGGGAAATAACAAGCCGCCACCAGCGAACTGACACCGACCAGAACTTTTTGTACGCGAGGCAATGTTCGCC‑ATAGCAAAAGCAAAA‑TAGCCGTAAA‑CGCAGCGGTGTAGAGCAAACTGAAGGGGTAACCTAAGCCg                                                                                                                                                 >  2:2035770/1‑149 (MQ=255)
      gCCTGAGCAAAAGGGAAATAACAAGCCGCCACCAGCGAACTGACACCGACCAGAACTTTTTGTACGCGAGGCAATGTTCGCC‑ATAGCAAAAGCAAAA‑TAGCCGTAAA‑CGCAGCGGTGTAGAGCAAACTGAAGGGGTAACCTAAGCCGAg                                                                                                                                               <  1:273553/149‑1 (MQ=255)
      gCCTGAGCAAAAGGGAAATAACAAGCCGCCACCAGCGAACTGACACCGACCAGAACTTTTTGTACGCGAGGCAATGTTCGCC‑ATAGCAAAAGCAAAA‑TAGCCGTAAA‑CGCAGCGGTGTAGAGCAAACTGAAGGGGTAACCTAAGCCGAg                                                                                                                                               <  2:4479752/149‑1 (MQ=255)
        cTGAGCAAAAGGGAAATAACAAGCCGCCACCAGCGAACTGACACCGACCAGAACTTTTTGTACGCGAGGCAATGTTCGCC‑ATAGCAAAAGCAAAA‑TAGCCGTAAA‑CGCAGCGGTGTAGAGCAAACTGAAGGGGTAACCTAAGCCGAGGt                                                                                                                                             >  2:5178194/1‑149 (MQ=255)
           aGCAAAAGGGAAATAACAAGCCGCCACCAGCGAACTGACACCGACCAGAACTTTTTGTACGCGAGGCAATGTTCGCC‑ATAGCAAAAGCAAAA‑TAGCCGTAAA‑CGCAGCGGTGTAGAGCAAACTGAAGGGGTAACCTAAGCCGAGGttaa                                                                                                                                          >  2:4758867/1‑149 (MQ=255)
              aaaaGGGAAATAACAAGCCGCCACCAGCGAACTGACACCGACCAGAACTTTTTGTACGCGAGGCAATGTTCGCC‑ATAGCAAAAGCAAAA‑TAGCCGTAAA‑CGCAGCGGTGTAGAGCAAACTGAAGGGGTAACCTAAGCCGAGGttaatta                                                                                                                                       <  1:3948223/149‑1 (MQ=255)
              aaaaGGGAAATAACAAGCCGCCACCAGCGAACTGACACCGACCAGAACTTTTTGTACGCGAGGCAATGTTCGCC‑ATAGCAAAAGCAAAA‑TAGCCGTAAA‑CGCAGCGGTGTAGAGCAAACTGAAGGGGTAACCTAAGCCGAGGttaatta                                                                                                                                       <  1:2546759/149‑1 (MQ=255)
               aaaGGGAAATAACAAGCCGCCACCAGCGAACTGACACCGACCAGAACTTTTTGTACGCGAGGCAATGTTCGCC‑ATAGCAAAAGCAAAA‑TAGCCGTAAA‑CGCAGCGGTGTAGAGCAAACTGAAGGGGTAACCTAAGCCGAGGttaattaa                                                                                                                                      >  1:3234559/1‑149 (MQ=255)
                  gggAAATAACAAGCCGCCACCAGCGAACTGACACCGACCAGAACTTTTTGTACGCGAGGCAATGTTCGCC‑ATAGCAAAAGCAAAA‑TAGCCGTAAA‑CGCAGCGGTGTAGAGCAAACTGAAGGGGTAACCTAAGCCGAGGTTAATTAATAg                                                                                                                                   >  2:4435944/1‑149 (MQ=255)
                     aaaTAACAAGCCGCCACCAGCGAACTGACACCGACCAGAACTTTTTGTACGCGAGGCAATGTTCGCC‑ATAGCAAAAGCAAAA‑TAGCCGTAAA‑CGCAGCGGTGTAGAGCAAACTGAAGGGGTAACCTACGCCGAGGTTAATTAATAGCGa                                                                                                                                >  2:2241848/1‑149 (MQ=255)
                      aaTAACAAGCCGCCACCAGCGAACTGACACCGACCAGAACTTTTTGTACGCGAGGCAATGTTCGCC‑ATAGCAAAAGCAAAA‑TAGCCGTAAA‑CGCAGCGGTGTAGAGCAAACTGAAGGGGTAACCTAAGCCGAGGTTAATTAATAGCGAc                                                                                                                               <  2:3612235/149‑1 (MQ=255)
                            acgccgccACCAGCGAACTGACACCGACCATAACTTTTTGTACGCGAGGCACTGTTCGCC‑ATAGTAAAAGCAAAA‑TAGCCGTAAA‑CGCAGCGGTGTAGAGCAAACTGATGGGGTAACCTAAGCCGAGGTTAATTAATAGCGACTGTaaa                                                                                                                         <  2:435310/147‑1 (MQ=255)
                            aaGCCGCCACCAGCGAACTGACACCGACCAGAACTTTTTGTACGCGAGGCAATGTTCGCC‑ATAGCAAAAGCAAAA‑TAGCCGTAAA‑CGCAGCGGTGTAGAGCAAACTGAAGGGGTAACCTAAGCCGAGGTTAATTAATAGCGACTGTaaa                                                                                                                         >  1:49472/1‑149 (MQ=255)
                             aGCCGCCACCAGCGAACTGACACCGACCAGAACTTTTTGTACGCGAGGCAATGTTCGCC‑ATAGCAAAAGCAAAA‑TAGCCGTAAA‑CGCAGCGGTGTAGAGCAAACTGAAGGGGTAACCTAAGCCGAGGTTAATTAATAGCGACTGTaaaa                                                                                                                        >  2:1371968/1‑149 (MQ=255)
                                 gccACCAGCGAACTGACACCGACCAGAACTTTTTGTACGCGAGGCAATGTTCGCC‑ATAGCAAAAGCAAAA‑TAGCCGTAAA‑CGCAGCGGTGTAGAGCAAACTGAAGGGGTAACCTAAGCCGAGGTTAATTAATAGCGACTGTAAAAAGTa                                                                                                                    >  2:4501147/1‑149 (MQ=255)
                                  ccaccaGCTAAAGTACACCGACCAGAACTTTTTGTACGCGAGGCAATGTTCGCA‑ATAGCAAAAGCAAAA‑TAGCCGTAAA‑CGCAGCGGTGTAGAGAAAACTGAAGGGTTAACCTAAGCCGAGGTTAATTAATAGCGACTGTAAAAAGTAg                                                                                                                   <  2:1817016/149‑1 (MQ=255)
                                    accaGCGAACTGACACCAACAATAACTTTTTGTACGCGAGGAAATGTTCGCA‑ATACAAAACCCAAAA‑TAGCCTAAAA‑CGCAGGGGTTTAGAGAAAACTGAAGGGTTAACCAAAGCCGAGTTTAATTAATAGCGACTGTAAAAAGTAGaa                                                                                                                 <  2:921932/149‑1 (MQ=255)
                                     ccaGCTAACTGACACCGACCAGAACTTTTTGTACGCTTGGCATTGTTCGCC‑ATAGAAAAAGAAAAA‑TAGCCGTAAA‑CGCAGGGTTGTAGAGCAAACTGAAGGGGTAACCTAAGCCGTGGTTATTTAATAGCGACTGTAAAAAGTAGAAc                                                                                                                <  2:4795588/149‑1 (MQ=255)
                                       cgccaaCTGACACCGACAAGAACTTTTTGTACGCGAGGAATTGTTCGCC‑ATAGCAAAACAAAAA‑TAGCCTTAAA‑CGCTGGGGTGTAGAGAAAACTGAAGGGGTAACCTAAGCCTAGTTTAATTAATAGCGACTGTAAAAAGTAGAACGc                                                                                                              <  1:4146159/145‑1 (MQ=255)
                                       aGCGAACTGACACCGACCAGAACTTTTTGTACGCGAGGCAATGTTCGCC‑ATAGCAAAAGCAAAA‑TAGCCGTAAA‑CGCAGCGGTGTAGAGCAAACTGAAGGGGTAACCTAAGCCGAGGTTAATTAATAGCGACTGTAAAAAGTAGAACGc                                                                                                              >  2:2585040/1‑149 (MQ=255)
                                        gCGAACTGACACCGACCAGAACTTTTTGTACGCGAGGCAATGTTCGCC‑ATAGCAAAAGCAAAA‑TAGCCGTAAA‑CGCAGCGGTGTAGAGCAAACTGAAGGGGTAACCTAAGCCGAGGTTAATTAATAGCGACTGTAAAAAGTAGAACGCa                                                                                                             <  2:1951543/149‑1 (MQ=255)
                                          gAACTGACACCGACCAGAACTTTTTGTACGCGAGGCAATGTTCGCC‑ATAGCAAAAGCAAAA‑TAGCCGTAAA‑CGCAGCGGTGTAGAGCAAACTGAAGGGGTAACCTAAGCCGAGGTTAATTAATAGCGACTGTAAAAAGTAGAACGCAGt                                                                                                           >  1:1866915/1‑149 (MQ=255)
                                           aaCTGACACCGACCAGAACTTTTTGTACGCGAGGCAATGTTCGCC‑ATAGCAAAAGCAAAA‑TAGCCGTAAA‑CGCAGCGGTGTAGAGCAAACTGAAGGGGTAACCTAAGCCGAGGTTAATTAATAGCGACTGTAAAAAGTAGAACGCAGTc                                                                                                          >  1:2181673/1‑149 (MQ=255)
                                           aaCTGACACCGACCAGAACTTTTTGTACGCGAGGCAATGTTCGCC‑ATAGCAAAAGCAAAA‑TAGCCGTAAA‑CGCAGCGGTGTAGAGCAAACTGAAGGGGTAACCTAAGCCGAGGTTAATTAATAGCGACTGTAAAAAGTAGAACGCAGTc                                                                                                          >  2:2380985/1‑149 (MQ=255)
                                           aaCTGACACCGACCAGAACTTTTTGTACGCGAGGCAATGTTCGCC‑ATAGCAAAAGCAAAA‑TAGCCGAAAA‑CGCAGCGGTGTAGAGCAAACTGAAGGGGTAACCTAAGCCGAGGTTAATTAATAGCGACTGTAAAAAGTAGAACGCAGTc                                                                                                          >  2:2099453/1‑149 (MQ=255)
                                              tGACCCCGCCAAGAACTTTTTGTCCGCGCGGCATGGTTCGCC‑ATCGCACAAGCAAAA‑TAGCCGTAAA‑CGCAGCGGTGTAGAGCAAACGGAAGGGGTAACCTAAGCCGTGGTTATTTAATAGCGACTGTAAAAAGTAGAACGCCGTCCAc                                                                                                       <  2:4083721/149‑1 (MQ=255)
                                              tGACCCCGACCACAACTTTTTGTACGCGAGGAAATGTTCGCC‑ATATCAAAAGCAAAA‑TAGCCGTAAA‑CGCAGCGGTGTATAGCAAACTGAAGGGGTAACCTAAGCCGTGGTTAATTAATAGCGACTGTAAAAAGTAGAACGCAGTCCAc                                                                                                       <  2:599512/149‑1 (MQ=255)
                                                   ccGACCACAACTTTTTGTCCGCGAGGCAATTTTCGCC‑ATAGCAAAAGAAAAA‑TAGCCGTAAA‑CGCAGCGGTGTAGAGCAAACTGACGGGGTAACCTACGCCGAGGTTAATTAATAGCGACTGTAAAAAGTAGAACGCAGTCCACGGGCt                                                                                                  <  2:1792119/149‑1 (MQ=255)
                                                     gACCAGAACTTTTTGTACGCGAGGCAATGTTCGCC‑ATAGCAAAAGCAAAA‑TAGCCGTAAA‑CGCAGCGGTGTAGAGCAAACTGAAGGGGTAACCTAAGCCGAGGTTAATTAATAGCGACTGTAAAAAGTAGAACGCAGTCCACGGGCTaa                                                                                                >  1:3452977/1‑149 (MQ=255)
                                                     gACCAGAACTTTTTGTACGCGAGGCAATGTTCGCC‑ATAGCAAAAGCAAAA‑TAGCCGTAAA‑CGCAGCGGTGTAGAGCAAACTGAAGGGGTAACCTAAGCCGAGGTTAATTAATAGCGACTGTAAAAAGTAGAACGCAGTCCACGGGCTaa                                                                                                >  1:3584286/1‑149 (MQ=255)
                                                     gACCAGAACTTTTTGTACGCGAGGCAATGTTCGCC‑ATAGCAAAAGCAAAA‑TAGCCGTAAA‑CGCAGCGGTGTAGAGCAAACTGAAGGGGTAACCTAAGCCGAGGTTAATTAATAGCGACTGTAAAAAGTAGAACGCAGTCCACGGGCTaa                                                                                                >  1:990156/1‑149 (MQ=255)
                                                      aCCAGAACTTTTTGTACGCGAGGCAATGTTCGCC‑ATAGCAAAAGCAAAA‑TAGCCGTAAA‑CGCAGCGGTGTAGAGCAAACTGAAGGGGTAACCTAAGCCGAGGTTAATTAATAGCGACTGTAAAAAGTAGAACGCAGTCCACGGGCTaaa                                                                                               >  2:3869865/1‑149 (MQ=255)
                                                       ccAGAACTTTTTGTACGCGAGGCAATGTTCGCC‑ATAGCAAAAGCAAAA‑TAGCCGTAAA‑CGCAGCGGTGTAGAGCAAACTGAAGGGGTAACCTAAGCCGAGGTTAATTAATAGCGACTGTAAAAAGTAGAACGCAGTCCACGGGCTAAAt                                                                                              >  2:5056108/1‑149 (MQ=255)
                                                          gAACTTTTTGTACGCGAGGCAATGTTCGCC‑ATAGCAAAAGCAAAA‑TAGCCGTAAA‑CGCAGCGGTGTAGAGCAAACTGAAGGGGTAACCTAAGCCGAGGTTAATTAATAGCGACTGTAAAAAGTAGAACGCAGTCCACGGGCTAAATAcc                                                                                           >  1:1414750/1‑149 (MQ=255)
                                                          gAACTTTTTGTACGCGAGGCAATGTTCGCC‑ATAGCAAAAGCAAAA‑TAGCCGTAAA‑CGCAGCGGTGTAGAGCAAACTGAAGGGGTAACCTAAGCCGAGGTTAATTAATAGCGACTGTAAAAAGTAGAACGCAGTCCACGGGCTAAATAcc                                                                                           >  1:52202/1‑149 (MQ=255)
                                                          gAACTTTTTGTACGCGAGGCAATGTTCGCC‑ATAGCAAAAGCAAAA‑TAGCCGTAAA‑CGCAGCGGTGTAGAGCAAACTGAAGGGGTAACCTAAGCCGAGGTTAATTAATAGCGACTGTAAAAAGTAGAACGCAGTCCACGGGCTAAATAcc                                                                                           >  1:4558366/1‑149 (MQ=255)
                                                           aaCTTTTTGTACGCGAGGCATTGTTCGCC‑AAAGCAAAAGCAAAC‑TAGCCGTAAA‑GGCAGCGGTGTAGAGCAAACTGACGGGGTACCCTACGCCGAGTTTATTTATTAGCGACTGTAAAAAGTAGAACGCAGTCCACGGGCTAAATAccc                                                                                          <  2:725210/149‑1 (MQ=255)
                                                           aaCTTTTTGTACGCGAGGCAATGTTCGCC‑ATAGCAAAAGCAAAA‑TAGCCGTAAA‑CGCAGCGGTGTAGAGCAAACTGAAGGGGTAACCTAAGCCGAGGTTAATTAATAGCGACTGTAAAAAGTAGTACGCAGTCCACGGGCGAAATAgcc                                                                                          >  2:3143554/1‑149 (MQ=255)
                                                             ttttttGTACGCTTGG‑AATTTTTGCCAATAGCAAAAGAAAAA‑TAGCGTTAAA‑CGCTGGGGTTTAGTGAAAACGTTAGGTGTAAACTACGCCGAGGTTAATTATTCGCGACTGTAAAAAGTAGAACGCAGTCCACGGGCTAAATACCCgg                                                                                        <  1:1130355/148‑1 (MQ=255)
                                                              tttttGTACGCGAGGCAATGTTCGCC‑ATAGCAAAAGCAAAA‑TAGCCGTAAA‑CGCAGGGGTGTAGAGCAAACTGACGGGGTAACCTAAGCCGAGGTTAATTAATCGCGACTGTAAAAAGTAGAACGCAGTCCACGGGCTAAATACCCGGc                                                                                       <  1:375088/149‑1 (MQ=255)
                                                              tttttGTACGCGAGGCAATGTTCGCC‑ATAGCAAAAGCAAAA‑TAGCCGTAAA‑CGCAGCGGTGTAGAGCAAACTGAAGGGGTAACCTAAGCCGAGGTTAATTAATAGCGACTGTAAAAAGTAGAACGCAGTCCACGGGCTAAATACCCGGc                                                                                       >  1:962203/1‑149 (MQ=255)
                                                                 ttGTCCGCGCGGCATTGTTCGCC‑ATA‑CAAAACCAAAA‑ATCCCCTAAACCGCCGCGGTGTAGAGAAAACGGACGGGGTAACATAAGCGTAGGTTAATTAACAGCGACTGTAAAAAGTAGAACGCAGTCCACGGGCTAAATACCCGGCTAc                                                                                    <  1:16957/149‑1 (MQ=255)
                                                                     aCGCGAGGCAATGTTCGCC‑ATAGCAAAAGCAAAA‑TAGCCGTAAA‑CGCAGCGGTGTAGAGCAAACTGAAGGGGTAACCTAAGCCGAGGTTAATTAATAGCGACTGTAAAAAGTAGAACGCAGTCCACGGGCTAAATACCCGGCTACGGGt                                                                                >  2:3960003/1‑149 (MQ=255)
                                                                     aCGCGAGGCAATGTTCGCC‑ATAGCAAAAGCAAAA‑TAGCCGTAAA‑CGCAGCGGTGTAGAGCAAACTGAAGGGGTAACCTAAGCCGAGGTTAATTAATAGCGACTGTAAAAAGTAGAACGCAGTCCACGGGCTAAATACCCGGCTACGGGt                                                                                >  1:4392605/1‑149 (MQ=255)
                                                                      cgcgAGGCAATGTTCGCC‑ATAGCAAAAGAAAAA‑TAGCCGTAAA‑CGCAGCGGTGTAGAGCAAACTGAAGGGGTAACCTAAGCCGAGGTTAATTAATAGCGACTGTAAAAAGTAGAACGCAGTCCACGGGCTAAATACCCGGCTACGGGTa                                                                               <  2:835527/149‑1 (MQ=255)
                                                                       gcgAGGCAATGTTCGCC‑ATAGCAAAAGCAAAA‑TAGCCGTAAA‑CGCAGCGGTGTAGAGCAAACTGAAGGGGTAACCTAAGCCGAGGTTAATTAATAGCGACTGTAAAAAGTAGAACGCAGTCCACGGGCTAAATACCCGGCTACGGGTaa                                                                              >  1:172549/1‑149 (MQ=255)
                                                                        cgAGGCAATGTTCGCC‑ATAGCAAAAGCAAAA‑TAGCCGTAAA‑CGCAGCGGTGTAGAGCAAACTGAAGGGGTAACCTAAGCCGAGGTTAATTAATAGCGACTGTAAAAAGTAGAACGCAGTCCACGGGCTAAATACCCGGCTACGGGTAAc                                                                             <  2:3771564/149‑1 (MQ=255)
                                                                           ggCAATGTTCGCC‑ATAGCAAAAGCAAAA‑TAGCCGTAAA‑CGCAGCGGTGTAGAGCAAACTGAAGGGGTAACCTACGCCGAGGTTAATTAATCGCGACTGTAAAAAGTAGAACGCAGTCCACGGGCTAAATACCCGGCTACGGGTAACAAg                                                                          <  1:2900051/149‑1 (MQ=255)
                                                                           ggCAATGTTCGCC‑ATAGCAAAAGCAAAA‑TAGCCGTAAA‑CGCAGCGGTGTAGAGCAAACTGAAGGGGTAACCTAAGCCGAGGTTAATTAATAGCGACTGTAAAAAGTAGAACGCAGTCCACGGGCTAAATACCCGGCTACGGGTAACAAg                                                                          >  2:5010428/1‑149 (MQ=255)
                                                                            gaaaTGTTCGCC‑ATACCAAAAGAAAAA‑TCGCCGTAAC‑CGCCGCGGTTTAGAGCAAACTTAAGGGTTAACCTAAGCCGAGTTTAATTAATAGCGACTGTAAAAAGTAGAACGCAGTCCACGGGCTAAATACCCGGCTACGGGTAACAAGc                                                                         <  1:3177297/147‑1 (MQ=255)
                                                                            gCAATGTTCGCC‑ATAGCAAAAGCAAAA‑TAGCCGTAAA‑CGCAGCGGTGTAGAGCAAACTGAAGGGGTAACCTAAGCCGAGGTTAATTAATAGCGACTGTAAAAAGTAGAACGCAGTCCACGGGCTAAATACCCGGCTACGGGTAACAAGc                                                                         >  1:5194433/1‑149 (MQ=255)
                                                                                tGTTCGCA‑ATAGCAAAACCAAAA‑TCGCCGTAAA‑CGCAGGGGTTTTGAGAAAACTTATGGGGTAACCTAAGCCGTGTTTAATTAATAGCGACTGTAAAAAGTAGAACGCAGTCCACGGGCTAAATACCCGGCTACGGGTAACAAGCGAtt                                                                     <  1:1654746/149‑1 (MQ=255)
                                                                                   tCGCC‑ATAGAAAAAGCAAAA‑TAGCCGTAAA‑CGCAGCGGTTTAGAGAAAACTTATGGGTTAACCTACGCCTAGTTTAATTAATAGCGACTGTAAAAAGTAGAACGCAGTCCACGGGCTAAATACCCGGCTACGGGTAACAAGCGATTCtt                                                                  <  1:2067521/149‑1 (MQ=255)
                                                                                   tCGCC‑ATACAAAAACCAAAA‑TCGCCTAAAA‑CCCAGGGGTGTAGAGAAAACTGAAGGGTTAACCTAAGCCGAGGTTAATTAATAGCGACTGTAAAAAGTAGAACGCAGTCCACGGGCTAAATACCCGGCTACGGGTAACAAGCGATTCtt                                                                  <  2:1287828/149‑1 (MQ=255)
                                                                                    cGCC‑ATCGCAAAAGAAAAA‑TAGCCGTAAA‑CGCAGCGGTGTAGAGCAAACTGACGGGGTAACCTAAGCCGTGGTTAATTAATAGCGACTGTAAAAAGTAGAACGCAGTCCACGGGCTAAATACCCGGCTACGGGTAACAAGCGATTCttt                                                                 <  1:825410/149‑1 (MQ=255)
                                                                                      cgaaatacaaaaacaaaatTAGCCGTAAA‑CGCTGGGTTTTATACCAAACTGATGGGTTAACCTATGCCTTGTTTAATTAATAGCGACTGTAAAAAGTAGAACGCAGTCCACGGGCTAAATACCCGGCTACGGGTAACAAGCGATTCttt                                                                 <  1:3551024/142‑1 (MQ=255)
                                                                                      cc‑ATAGCAAAAGCAAAA‑TAGCCGTAAA‑CGCAGCGGTGTAGAGCAAACTGAAGGGGTAACCTAAGCCGAGGTTAATTAATAGCGACTGTAAAAAGTAGAACGCAGTCCACGGGCTAAATACCCGGCTACGGGTAACAAGCGATTCTTTGa                                                               >  1:10780/1‑149 (MQ=255)
                                                                                          tAGAAAAACAAAAA‑TAGCCTTAAA‑CGCAGCGTTTTAGAGAAAACTGATGGGTTAACCTACGCCTTGTTTAATTAATCGCGACTGTAAAAAGTAGAACGCAGTCCACGGGCTAAATACCCGGCTACGGGTAACAAGCGATTCTTTGAggg                                                            <  1:1004754/149‑1 (MQ=255)
                                                                                            gcaaaagcaaaa‑TAGCCGTAAA‑CGCAGCGGTGTAGAGCAAACTGAAGGGGTAACCTAAGCCGAGGTTAATTAATAGCGACTGTAAAAAGTAGAACGCAGTCCACGGGCTAAATACCCGGCTACGGGTAACAAGCGATTCTTTGAGGGtt                                                          >  2:2842353/1‑149 (MQ=255)
                                                                                            gcaaaagcaaaa‑TAGCCGTAAA‑CGCAGCGGTGTAGAGCAAACTGAAGGGGTAACCTAAGCCGAGGTTAATTAATAGCGACTGTAAAAAGTAGAACGCAGTCCACGGGCTAAATACCCGGCTACGGGTAACAAGCGATTCTTTGAGGGtt                                                          >  1:1252991/1‑149 (MQ=255)
                                                                                            gcaaaagaaaaa‑TAGCCGTAAA‑CGCAGCGGTGTAGAGCAAACTGACGGGGTAACCTAAGCCGAGGTTAATTAATAGCGACTGTAAAAAGTAGAACGCAGTCCACGGGCTAAATACCCGGCTACGGGTAACAAGCGATTCTTTGAGGGtt                                                          <  2:2708643/149‑1 (MQ=255)
                                                                                              aaaagcaaaa‑TAGCCGTAAA‑CGCAGCGGTGTAGAGCAAACTGAAGGGGTAACCTAAGCCGAGGTTAATTAATAGCGACTGTAAAAAGTAGAACGCTGTCCACGGGCTAAATACCCGGCTACGGGTAACAAGCGATTCTTTGAGGGTTaa                                                        >  2:1929277/1‑149 (MQ=255)
                                                                                               aaagcaaaa‑TAGCCGTAAA‑CGCAGCGGTGTAGAGCAAACTGAAGGGGTAACCTAAGCCGAGGTTAATTAATAGCGACTGTAAAAAGTAGAACGCAGTCCACGGGCTAAATACCCGGCTACGGGTAACAAGCGATTCTTTGAGGGTTaaa                                                       >  1:2045384/1‑149 (MQ=255)
                                                                                                 agcaaaa‑TAGCCGTAAA‑CGCAGCGGTGTAGAGCAAACTGAAGGGGTAACCTAAGCCGAGGTTAATTAATAGCGACTGTAAAAAGTAGAACGCAGTCCACGGGCTAAATACCCGGCTACGGGTAACAAGCGATTCTTTGAGGGTTAAAtt                                                     >  1:1914014/1‑149 (MQ=255)
                                                                                                   caaaa‑TAGCCTTAAC‑CGCAGCGGTGTAGAGCAAACTGAAGGGGTAACCTAAGCCGTGGTTAATTAATAGCGACTGTAAAAAGTAGAACGCAGTCCACGGGCTAAATACCCGGCTACGGGTAACAAGCGATTCTTTGAGGGTTAAATTCa                                                   <  2:2761317/149‑1 (MQ=255)
                                                                                                    aaaa‑TCGCCTTAAA‑CGCAGCGGTGTAGAGCAAACTGATGGGGTAACCTAAGCCGTGTTTAATTAATAGCGACTGTAAAAAGTAGAACGCAGTCCACGGGCTAAATACCCGGCTACGGGTAACAAGCGATTCTTTGAGGGTTAAATTCat                                                  <  1:1527687/149‑1 (MQ=255)
                                                                                                    aaaa‑TAGCCGTAAA‑CGCAGCGGTGTAGAGCAAACTGAAGGGGTAACCTAAGCCGAGGTTAATTAATAGCGACTGTAAAAAGTAGAACGCAGTCCACGGGCTAAATACCCGGCTACGGGTAACAAGCGATTCTTTGAGGGTTAAATTCat                                                  >  2:4237671/1‑149 (MQ=255)
                                                                                                       a‑TAGCCGTAAA‑CGCAGCGGTGTAGAGCAAACTGAAGGGGTAACCTAAGCCGAGGTTAATTAATAGCGACTGTAAAAAGTAGAACGCAGTCCACGGGCTAAATACCCGGCTACGGGTAACAAGCGATTCTTTGAGGGTTAAATTCATATg                                               >  2:487259/1‑149 (MQ=255)
                                                                                                         tAGCCGTAAA‑CGCTGCGGTGTAGAGCAAACTGATGGGGTAACCTAAGCCGAGGTTAATTAATAGCGACTGTAAAAAGTAGAACGCAGTCCACGGGCTAAATACCCGGCTACGGGTAACAAGCGATTCTTTGAGGGTTAAATTCATATGc                                              <  2:1411465/149‑1 (MQ=255)
                                                                                                          aGCCGTAAA‑CGCAGCGGTGTAGAGCAAACTGAAGGGGTAACCTAAGCCGAGGTTAATTAATAGCGACTGTAAAAAGTAGAACGCAGTCCACGGGCTAAATACCCGGCTACGGGTAACAAGCGAGTCTTTGAGGGTTAAATTCATATGcc                                             >  2:4309559/1‑149 (MQ=255)
                                                                                                           gCCGTAAA‑CGCAGCGTTGTATAGCAAACTGACGGGGTACCCTAAGCCGAGTTTAATTAATAGCGACTGTAAAAAGTAGAACGCAGTCCACGGGCTAAATACCCGGCTACGGGTAACAAGCGATTCTTTGAGGGTTAAATTCATATGCCa                                            <  2:3497969/149‑1 (MQ=255)
                                                                                                           gCCGTAAA‑CGCAGCGGTGTAGAGCAAACTGAAGGGGTAACCTAAGCCGAGGTTAATTAATAGCGACTGTAAAAAGTAGAACGCAGTCCACGGGCTAAATACCCGGCTACGGGTAACAAGCGATTCTTTGAGGGTTAAATTCATATGCCa                                            >  2:3914703/1‑149 (MQ=255)
                                                                                                             cGTAAA‑CGCAGCGGTGTAGAGCAAACTGACGGGTTAACCTAAGCCGAGGTTAATTAATAGCGACTGTAAAAAGTAGAACGCAGTCCACGGGCTAAATACCCGGCTACGGGTAACAAGCGATTCTTTGAGGGTTAAATTCATATGCCACt                                          <  2:4628109/149‑1 (MQ=255)
                                                                                                              ttAAA‑CGCAGCGGTTTAGAGCAAACTGAAGGGGTAACCTAAGCCGAGGTTAATTAATAGCGACTGTAAAAAGTAGAACGCAGTCCACGGGCTAAATACCCGGCTACGGGTAACAAGCGATTCTTTGAGGGTTAAATTCATATGCCACTa                                         <  2:1091033/148‑1 (MQ=255)
                                                                                                              gTAAA‑CGCAGCGGTGTAGAGCAAACTGAAGGGGTAACCTAAGCCGAGGTTAATTAATAGCGACTGTAAAAAGTAGAACGCAGTCCACGGGCTAAATACCCGGCTACGGGTAACAAGCGATTCTTTGAGGGTTAAATTCATATGCCACTa                                         >  2:4698302/1‑149 (MQ=255)
                                                                                                              gTAAA‑CGCAGCGGTGTAGAGCAAACTGAAGGGGTAACCTAAGCCGAGGTTAATTAATAGCGACTGTAAAAAGTAGAACGCAGTCCACGGGCTAAATACCCGGCTACGGGTAACAAGCGATTCTTTGAGGGTTAAATTCATATGCCACTa                                         >  1:3263467/1‑149 (MQ=255)
                                                                                                               tAAA‑CGCAGCGGTGTAGAGCAAACTGAAGGGGTAACCTAAGCCGAGGTTAATTAATAGCGACTGTAAAAAGTAGAACGCAGTCCACGGGCTAAATACCCGGCTACGGGTAACAAGCGATTCTTTGAGGGTTAAATTCATATGCCACTAt                                        >  1:647773/1‑149 (MQ=255)
                                                                                                                        gCGGTGTAGAGCAAACTGAAGGGGTAACCTAAGCCGAGGTTAATTAATAGCGACTGTAAAAAGTAGAACGCAGTCCACGGGCTAAATACCCGGCTACGGGTAACAAGCGATTCTTTGAGGGTTAAATTCATATGCCACTATCGTAACaa                                >  1:1223940/1‑149 (MQ=255)
                                                                                                                        gCGGTGTAGAGCAAACTGAAGGGGTAACCTAAGCCGAGGTTAATTAATAGCGACTGTAAAAAGTAGAACGCAGTCCACGGGCTAAATACCCGGCTACGGGTAACAAGCGATTCTTTGAGGGTTAAATTCATATGCCACTATCGTAACaa                                >  1:1335331/1‑149 (MQ=255)
                                                                                                                        gCGGTGTAGAGCAAACTGAAGGGGTAACCTAAGCCGAGGTTAATTAATAGCGACTGTAAAAAGTAGAACGCAGTCCACGGGCTAAATACCCGGCTACGGGTAACAAGCGATTCTTTGAGGGTTAAATTCATATGCCACTATCGTAACaa                                >  1:3800714/1‑149 (MQ=255)
                                                                                                                         cGGTGTAGAGCAAACTGATGGGGTAACCTAAGCCGTGGTTAATTAATCGCGACTGTAAAAAGTAGAACGCAGTCCACGGGCTAAATACCCGGCTACGGGTAACAAGCGATTCTTTGAGGGTTAAATTCATATGCCACTATCGTAACaaa                               <  1:753894/149‑1 (MQ=255)
                                                                                                                          ggTGTAGAGCAAACTGAAGGGGTAACCTAAGCCGAGGTTAATTAATAGCGACTGTAAAAAGTAGAACGCAGTCCACGGGCTAAATACCCGGCTACGGGTAACAAGCGATTCTTTGAGGGTTAAATTCATATGCCACTATCGTAACAAAc                              >  1:1235876/1‑149 (MQ=255)
                                                                                                                           gtgtAGAGCAAACTGAAGGGGTAACCTAAGCCGAGGTTAATTAATAGCGACTGTAAAAAGTAGAACGCAGTCCACGGGCTAAATACCCGGCTACGGGTAACAAGCGATTCTTTGAGGGTTAAATTCATATGCCACTATCGTAACAAACg                             >  1:3103712/1‑149 (MQ=255)
                                                                                                                            tgtAGAGCAAACTGAAGGGGTAACCTAAGCCGAGGTTAATTAATAGCGACTGTAAAAAGTAGAACGCAGTCCACGGGCTAAATACCCGGCTACGGGTAACAAGCGATTCTTTGAGGGTTAAATTCATATGCCACTATCGTAACAAACGc                            >  2:2687673/1‑149 (MQ=255)
                                                                                                                                 agaaaaCTGAAGGGGTAACCTAAGCCGAGGTTAATTAATAGCGACTGTAAAAAGTAGAACGCAGTCCACGGGCTAAATACCCGGCTACGGGTAACAAGCGATTCTTTGAGGGTTAAATTCATATGCCACTATCGTAACAAACGCCAtgt                       <  2:1654169/146‑1 (MQ=255)
                                                                                                                                     aaCTGAAGGGGTAACCTAAGCCGAGGTTAATTAATAGCGACTGTAAAAAGTAGAACGCAGTCCACGGGCTAAATACCCGGCTACGGGTAACAAGCGATTCTTTGAGGGTTAAATTCATATGCCACTATCGTAACAAACGCCATGTGCtt                   >  2:758008/1‑149 (MQ=255)
                                                                                                                                      aCTGAAGGGGTAACCTAAGCCGAGGTTAATTAATAGCGACTGTAAAAAGTAGAACGCAGTCCACGGGCTAAATACCCGGCTACGGGTAACAAGCGATTCTTTGAGGGTTAAATTCATATGCCACTATCGTAACAAACGCCATGTGCTTa                  >  2:4493350/1‑149 (MQ=255)
                                                                                                                                       cTGAAGGGGTAACCTACGCCGAGGTTAATTAATAGCGACTGTAAAAAGTAGAACGCAGTCCACGGGCTAAATACCCGGCTACGGGTAACAAGCGATTCTTTGAGGGTTAAATTCATATGCCACTATCGTAACAAACGCCATGTGCTTAc                 >  1:2133495/1‑149 (MQ=255)
                                                                                                                                        tGAAGGGGTAACCTAAGCCGAGGTTAATTAATAGCGACTGTAAAAAGTAGAACGCAGTCCACGGGCTAAATACCCGGCTACGGGTAACAAGCGATTCTTTGAGGGTTAAATTCATATGCCACTATCGTAACAAACGCCATGTGCTTAcc                >  2:3278647/1‑149 (MQ=255)
                                                                                                                                          aaGGGGTAACCTAAGCCGAGGTTAATTAATAGCGACTGTAAAAAGTAGAACGCAGTCCACGGGCTAAATACCCGGCTACGGGTAACAAGCGATTCTTTGAGGGTTAAATTCATATGCCACTATCGTAACAAACGCCATGTGCTTACCCt              >  1:2461585/1‑149 (MQ=255)
                                                                                                                                             gggTAACCTAAGCCGAGGTTAATTAATAGCGACTGTAAAAAGTAGAACGCAGTCCACGGGCTAAATACCCGGCTACGGGTAACAAGCGATTCTTTGAGGGTTAAATTCATATGCCACTATCGTAACAAACGCCATGTGCTTACCCTGGc           >  2:2522685/1‑149 (MQ=255)
                                                                                                                                             gggTAACCTAAGCCGAGGTTAATTAATAGCGACTGTAAAAAGTAGAACGCAGTCCACGGGCTAAATACCCGGCTACGGGTAACAAGCGATTCTTTGAGGGTTAAATTCATATGCCACTATCGTAACAAACGCCATGTGCTTACCCTGGc           >  2:4955720/1‑149 (MQ=255)
                                                                                                                                               gTAACCTAAGCCGAGGTTAATTAATAGCGACTGTAAAAAGTAGAACGCAGTCCACGGGCTAAATACCCGGCTACGGGTAACAAGCGATTCTTTGAGGGTTAAATTCATATGCCACTATCGTAACAAACGCCATGTGCTTACCCTGGCGt         <  1:4691880/149‑1 (MQ=255)
                                                                                                                                                  aCCTAAGCCGAGGTTAATTAATAGCGACTGTAAAAAGTAGAACGCAGTCCACGGGCTAAATACCCGGCTACGGGTAACAAGCGATTCTTTGAGGGTTAAAGTCATATGCCACTATCGTAACAAACGCCATGTGCTTACCCTGGCGTCaa      >  1:336935/1‑149 (MQ=255)
                                                                                                                                                   ccTAAGCCGAGGTTAATTAATAGCGACTGTAAAAAGTAGAACGCAGTCCACGGGCTAAATACCCGGCTACGGGTAACAAGCGATTCTTTGAGGGTTAAATTCATATGCCACTATCGTAACAAACGCCATGTGCTTACCCTGGCGTCAAg     >  2:295620/1‑149 (MQ=255)
                                                                                                                                                     tAAGCCGAGGTTAATTAATAGCGACTGTAAAAAGTAGAACGCAGTCCACGGGCTAAATACCCGGCTACGGGTAACAAGCGATTCTTTGAGGGTTAAATTCATATGCCACTATCGTAACAAACGCCATGTGCTTACCCTGGCGTCAAggg   <  2:331922/149‑1 (MQ=255)
                                                                                                                                                      aaGCCGAGGTTAATTAATAGCGACTGTAAAAAGTAGAACGCAGTCCACGGGCTAAATACCCGGCTACGGGTAACAAGCGATTCTTTGAGGGTTAAATTCATATGCCACTATCGTAACAAACGCCATGTGCTTACCCTGGCGTCAAGGGt  >  1:2517459/1‑149 (MQ=255)
                                                                                                                                                      aaGCCGAGGTTAATTAATAGCGACTGTAAAAAGCAGAACGCAGTCCACGGGCTAAATACCCGGCTACGGGTAACAAGCGATTCTTTGAGGGTTAAATTCATATGCCACTATCGTAACAAACGCCATGTGCTTACCCTGGCGTCAAGGGt  <  2:682060/149‑1 (MQ=255)
                                                                                                                                                       |                                                                                                                                                   
CCGTAGGCCTGAGCAAAAGGGAAATAACAAGCCGCCACCAGCGAACTGACACCGACCAGAACTTTTTGTACGCGAGGCAATGTTCGCC‑ATAGCAAAAGCAAAA‑TAGCCGTAAA‑CGCAGCGGTGTAGAGCAAACTGAAGGGGTAACCTAAGCCGAGGTTAATTAATAGCGACTGTAAAAAGTAGAACGCAGTCCACGGGCTAAATACCCGGCTACGGGTAACAAGCGATTCTTTGAGGGTTAAATTCATATGCCACTATCGTAACAAACGCCATGTGCTTACCCTGGCGTCAAGGGT  >  CP009273/847806‑848101

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑