Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 107,220 A→T 14.3% K819* (AAA→TAA)  secA → preprotein translocase subunit, ATPase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273107,2200AT14.3% 929.8 / 28.0 323K819* (AAA→TAA) secApreprotein translocase subunit, ATPase
Reads supporting (aligned to +/- strand):  ref base A (84/182);  new base T (39/3);  total (138/185)
Fisher's exact test for biased strand distribution p-value = 1.54e-14
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 328 total aligned reads displayed.

AACGCTTGACTCCCTGTGGAAAGAGCACCTGGCAGCGATGGACTATCTGCGTCAGGGTATCCACCTGCGTGGCTACGCACAGAAAGATCCGAAGCAGGAATACAAACGTGAATCGTTCTCCATGTTTGCAGCGATGCTGGAGTCGTTGAAATATGAAGTTATCAGTACGCTGAGCAAAGTTCAGGTACGTATGCCTGAAGAGGTTGAGGAGCTGGAACAACAGCGTCGTATGGAAGCCGAGCGTTTAGCGCAAATGCAGCAGCTTAGCCATCAGGATGACGACTCTGCAGCCGCAG  >  CP009273/107072‑107367
                                                                                                                                                    |                                                                                                                                                   
aaCGCTTGACTCCCTGTGGAAAGAGCACCTGGCAGCGATGGACTATCTGCGTCAGGGTATCCACCTGCGTGGCTACGCACAGAAAGATCCGAAGCAGGAATACAAACGTGAATCGTTCTCCATGTTTGCAGCGATGCTGGAGTCGTGGa                                                                                                                                                     >  2:209442/1‑149 (MQ=255)
aaCGCTTGACTCCCTGTGGAAAGAGCACCTGGCAGCGATGGACTATCTGCGTCAGGGTATCCACCTGCGTGGCTACGCACAGAAAGATCCGAAGCAGGAATACAAACGTGAATCGTTCTCCATGTTTGCAGCGATGCTGGAGTCGTGGa                                                                                                                                                     >  2:4942887/1‑149 (MQ=255)
   gCTTGACTCCCTGTGGAAAGAGCACCTGGCAGCGATGGACTATCTGCGTCAGGGTATCCACCTGCGTGGCTACGCACAGAAAGATCCGAAGCAGGAATACAAACGTGAATCGTTCTCCATGTTTGCAGCGATGCTGGAGTCGTTGAAat                                                                                                                                                  <  2:387968/149‑1 (MQ=255)
     ttGACTCCCTGTGGAAAGAGCACCTGGCAGCGATGGACTATCTGCGTCAGGGTATCCACCTGCGTGGCTACGCACAGAAAGATCCGAAGCAGGAATACAAACGTGAATCGTTCTCCATGTTTGCAGCGATGCTGGAGTCGTTGAAatat                                                                                                                                                >  2:4964962/1‑149 (MQ=255)
       gACTCCCTGTGGAAAGAGCACCTGGCAGCGATGGACTATCTGCGTCAGGGTATCCACCTGCGTGGCTACGCACAGAAAGATCCGAAGCAGGAATACAAACGTGAATCGTTCTCCATGTTTGCAGCGATGCTGGAGTCGTGGTGATATgt                                                                                                                                              >  1:4835388/1‑148 (MQ=255)
         cTCCCTGTGGAAAGAGCACCTGGCAGCGATGGACTATCTGCGTCAGGGTATCCACCTGCGTGGCTACGCACAGAAAGATCCGAAGCAGGAATACAAACGTGAATCGTTCTCCATGTTTGCAGCGATGCTGGAGTCGTTGAAATATGAAg                                                                                                                                            >  2:2690094/1‑149 (MQ=255)
          tCCCTGTGGAAAGAGCACCTGGCAGCGATGGACTATCTGCGTCAGGGTATCCACCTGCGTGGCTACGCACAGAAAGATCCGAAGCAGGAATACAAACGTGAATCGTTCTCCATGTTTGCAGCGATGCTGGAGTCGTTGAAATATTAAGt                                                                                                                                           >  2:3268362/1‑149 (MQ=255)
          tCCCTGTGGAAAGAGCACCTGGCAGCGATGGACTATCTGCGTCAGGGTATCCACCTGCGTGGCTACGCACAGAAAGATCCGAAGCAGGAATACAAACGTGAATCGTTCTCCATGTTTGCAGCGATGCTGGAGTCGTTGAAATATGAAGt                                                                                                                                           <  2:3005257/149‑1 (MQ=255)
          tCCCTGTGGAAAGAGCACCTGGCAGCGATGGACTATCTGCGTCAGGGTATCCACCTGCGTGGCTACGCACAGAAAGATCCGAAGCAGGAATACAAACGTGAATCGTTCTCCATGTTTGCAGCGATGCTGGAGTCGTTGAAATATGAAGt                                                                                                                                           <  2:2654826/149‑1 (MQ=255)
          tCCCTGTGGAAAGAGCACCTGGCAGCGATGGACTATCAGCGTCAGGGTATCCACCTGCGTGGCTACGCACAGAAAGATCCGAAGCATGAATACAAACGTGAATCGTTCTCCATGTTTGCAGCGATGCTGGAGTCGTTTAAATATGAAGt                                                                                                                                           <  2:4903344/149‑1 (MQ=255)
             cTGTGGAAAGAGCACCTGGCAGCGATGGACTATCTGCGTCAGGGTATCCACCTGCGTGGCTACGCACAGAAAGATCCGAAGCAGGAATACAAACGTGAATCGTTCTCCATGTTTGCAGCGATGCTGGAGTCGTTGATATATTAATTTAt                                                                                                                                        >  1:1349037/1‑149 (MQ=255)
             cTGTGGAAAGAGCACCTGGCAGCGATGGACTATCTGCGTCAGGGTATCCACCTGCGTGGCTACGCACAGAAAGATCCGAAGCAGGAATACAAACGTGAATCGTTCTCCATGTTTGCAGCGATGCTGGAGTCGTTGAAATATGAAGTTAt                                                                                                                                        <  1:3755627/149‑1 (MQ=255)
              tgtgGAAAGAGCACCTGGCAGCGATGGACTATCTGCGTCAGGGTATCCACCTGCGTGGCTACGCACAGAAAGATCCGAAGCAGGAATACAAACGTGAATCGTTCTCCATGTTTGCAGCGATGCTGGAGTCGTTGAAATATGAAGTTATc                                                                                                                                       <  1:3553377/149‑1 (MQ=255)
              tgtgGAAAGAGCACCTGGCAGCGATGGACTATCTGCGTCAGGGTATCCACCTGCGTGGCTACGCACAGAAAGATCCGAAGCAGGAATACAAACGTGAATCGTTCTCCATGTTTGCAGCGATGCTGGAGTCGTTGAAATATGAAGTTATc                                                                                                                                       <  2:3950720/149‑1 (MQ=255)
                 ggAAAGAGCACCTGGCAGCGATGGACTATCTGCGTCAGGGTATCCACCTGCGTGGCTACGCACAGAAAGATCCGAAGCAGGAATACAAACGTGAATCGTTCTCCATGTTTGCAGCGATGCTGGAGTCGTTGAAATATGAAGTTATCAGt                                                                                                                                    <  2:1400612/149‑1 (MQ=255)
                    aaGAGCACCTGGCAGCGATGGACTATCTGCGTCAGGGTATCCACCTGCGTGGCTACGCACAGAAAGATCCGAAGCAGGAATACAAACGTGAATCGTTCTCCATGTTTGCAGCGATGCTGGAGTCGTTGAAATATGAATTTATCAGTACg                                                                                                                                 >  2:513087/1‑149 (MQ=255)
                      gagCACCTGGCAGCGATGGACTATCTGCGTCAGGGTATCCACCTGCGTGGCTACGCACAGAAAGATCCGAAGCAGGAATACAAACGTGAATCGTTCTCCATGTTTGCAGCGATGCTGGAGTCGTTGAAATATGAAGTTATCAGTACGCt                                                                                                                               <  1:3170702/149‑1 (MQ=255)
                       agCACCTGGCAGCGATGGACTATCTGCGTCAGGGTATCCACCTGCGTGGCTACGCACAGAAAGATCCGAAGCAGGAATACAAACGTGAATCGTTCTCCATGTTTGCAGCGATGCTGGAGTCGTTGAAATATGAAGTTATCAGTACGCTg                                                                                                                              <  2:2936813/149‑1 (MQ=255)
                        gCACCTGGCAGCGATGGACTATCTGCGTCAGGGTATCCACCTGCGTGGCTACGCACAGAAAGATCCGAAGCAGGAATACAAACGTGAATCGTTCTCCATGTTTGCAGCGATGCTGGAGTCGTTGAAATATGAAGTTATCAGTACGCTGa                                                                                                                             <  2:2482594/149‑1 (MQ=255)
                         cACCTGGCAGCGATGGACTATCTGCGTCAGGGTATCCACCTGCGTGGCTACGCACAGAAAGATCCGAAGCAGGAATACAAACGTGAATCGTTCTCCATGTTTGCAGCGATGCTGGAGTCGTTGAAATATGAAGTTATCAGTACGCTGAg                                                                                                                            <  1:4900146/149‑1 (MQ=255)
                         cACCTGGCAGCGATGGACTATCTGCGTCAGGGTATCCACCTGCGTGGCTACGCACAGAAAGATCCGAAGCAGGAATACAAACGTGAATCGTTCTCCATGTTTGCAGCGATGCTGGAGTCGTTGAAATATGAAGTTATCAGTACGCTGAg                                                                                                                            <  1:2509217/149‑1 (MQ=255)
                          aCCTGGCAGCGATGGACTATCTGCGTCAGGGTATCCACCTGCGTGGCTACGCACAGAAAGATCCGAAGCAGGAATACAAACGTGAATCGTTCTCCATGTTTGCAGCGATGCTGGAGTCGTTGAAATATGAAGTTATCAGTACGcgtagc                                                                                                                           >  1:4712420/1‑144 (MQ=255)
                              ggCAGCGATGGACTATCTGCGTCAGGGTATCCACCTGCGTGGCTACGCACAGAAAGATCCGAAGCAGGAATACAAACGTGAATCGTTCTCCATGTTTGCAGCGATGCTGGAGTCGTTGAAATATGAAGTTATCAGTACGCTGAGCAAAg                                                                                                                       <  1:4767902/149‑1 (MQ=255)
                                  gCGATGGACTATCTGCGTCAGGGTATCCACCTGCGTGGCTACGCACAGAAAGATCCGAAGCAGGAATACAAACGTGAATCGTTCTCCATGTTTGCAGCGATGCTGGAGTCGTTGAAATATGAAGTTATCAGTACGCTGAGCAAAGTTCa                                                                                                                   <  1:2208702/149‑1 (MQ=255)
                                    gATGGACTATCTGCGTCAGGGTATCCACCTGCGTGGCTACGCACAGAAAGATCCGAAGCAGGAATACAAACGTGAATCGTTCTCCATGTTTGCAGCGATGCTGGAGTCGTTGAAATATGAAGTTATCAGTACGCTGAGCAAAGTTCAgg                                                                                                                 <  1:3088560/149‑1 (MQ=255)
                                     aTGGACTATCTGCGTCAGGGTATCCACCTGCGTGGCTACGCACAGAAAGATCCGAAGCAGGAATACAAACGTGACTCGTTCTCCATGTTTGCAGCGATGCTGGAGTCGTTGTAATATTAAATTATAAATAAGCCGAGCAAAGttaatgt                                                                                                                >  1:1529422/1‑144 (MQ=255)
                                     aTGGACTATATGCGTCAGGGTATCCAACTGCGTGGCTACGCACAGAAAGATCCGAAGCAGGAATACAATCGTGAATCGTTCTCCATGTTTGCAGCGATGCTGGAGTCGTTGAAATATGAAGTTATCAGTACGCTGAGCAAAGTTCAGGt                                                                                                                <  2:2538343/149‑1 (MQ=255)
                                      tGGACTATCTGCGTCAGGGTATCCACCTGCGTGGCTACGCACAGAAAGATCCGAAGCAGGAATACAAACGTGAATCGTTCTCCATGTTTGCAGCGATGCTGGAGTCGTTGAAATATGAAGTTATCAGTACGCTGAGCAAAGTTCAGGTa                                                                                                               <  2:662236/149‑1 (MQ=255)
                                         aCTATCTGCGTCAGGGTATCCACCTGCGTGGCTACGCACAGAAAGATCCGAAGCAGGAATACAAACGTGAATCGTTCTCCATGTTTGCAGCGATGCTGGAGTCGTGGTAATATGTTGTTTTCAGTAAGCTGTGCCAAGTTCAGGTTCGt                                                                                                            >  1:26993/1‑149 (MQ=255)
                                          cTATCTGCGTCAGGGTATCCACCTGCGTGGCTACGCACAGAAAGATCCGAAGCAGGAATACAAACGTGAATCGTTCTCCATGTTTGCAGCGATGCTGGAGTCGTTGAAATATGAAGTTATCAGTACGCTGAGCAAAGTTCAGGTACGTa                                                                                                           <  1:4058393/149‑1 (MQ=255)
                                          cTATCTGCGTCAGGGTATCCACCTGCGTGGCTACGCACAGAAAGATCCGAAGCAGGAATACAAACGTGAATCGTTCTCCATGTTTGCAGCGATGCTGGAGTCGTTGAAAAATTAAGTTATCAGTACGCATAGCAAAGTTAAGGTACGTa                                                                                                           >  2:4377650/1‑149 (MQ=255)
                                           tATCTGCGTCAGGGTATCCACCTGCGTGGCTACGCACAGAAAGATCCGAAGCAGGAATACAAACGTGAATCGTTCTCCATGTTTGCAGCGATGCTGGAGTCGTTGAAATATGAAGTTATCAGTACGCTGAGCAAAGTTCAGGTACGTAt                                                                                                          <  1:2320967/149‑1 (MQ=255)
                                                  gTCAGGGTATCCACCTGCGTGGCTACGCACAGAAAGATCCGAAGCAGGAATACAAACGTGAATCGTTCTCCATGTTTGCAGCGATGCTGGAGTCGTGGAAATATGTAGTTATCAGTACGCGGAGCAAAGTTCCGGGACGTATGCCGGac                                                                                                   >  1:4995943/1‑148 (MQ=255)
                                                   tCAGGGTATCCACCTGCGTGGCTACGCACAGAAAGATCCGAAGCAGGAATACAAACGTGAATCGTTCTCCATGTTTGCAGCGATGCTGGAGTCGTGGCAATATGAAGGTATCAGTACGCGGAGCCAAGTTCAGGTACGTATGCCTGCag                                                                                                  >  2:3749182/1‑149 (MQ=255)
                                                     aGGGTATCCACCTGCGTGGCTACGCACAGAAAGATCCGAAGCAGGAATACAAACGTGAATCGTTCTCCATGTTTGCAGCGATGCTGGAGTCGTTGAAATATGAAGTTATCAGTACGCTGAGCAAAGTTCAGGTACGTATGCCTGAagag                                                                                                <  1:894256/149‑1 (MQ=255)
                                                     aGGGTATCCACCTGCGTGGCTACGCACAGAAAGATCCGAAGCAGGAATACAAACGTGAATCGTTCTCCATGTTTGCAGCGATGCTGGAGTCGTTGAAATATGAAGTTATCAGTACGCTGAGCAAAGTTCAGGTACGTATGCCTGAagag                                                                                                <  1:1782746/149‑1 (MQ=255)
                                                      gggTATCCACCTGCGTGGCTACGCACAGAAAGATCCGAAGCAGGAATACAAACGTGAATCGTTCTCCATGTTTGCAGCGATGCTGGAGTCGTGGTAATATGAAGTTATCACGACGCGGAGCAAAGTTCAGGTACGTATGCCGGAAGAgg                                                                                               >  2:1735400/1‑149 (MQ=255)
                                                       ggTATCCACCTGCGTGGCTACGCACAGAAAGATCCGAAGCAGGAATACAAACGTGAATCGTTCTCCATGTTTGCAGCGATGCTGGAGTCGTTGAAATATGAAGTTATCAGTACGCTGAGCAAAGTTCAGGTACGTATGCCTGAAGAGGt                                                                                              <  2:3605634/149‑1 (MQ=255)
                                                        gTATCCACCTGCGTGGCTACGCACAGAAAGATCCGAAGCAGGAATACAAACGTGAATCGTTCTCCATGTTTGCAGCGATGCTGGAGTCGTTGTATTATGTAGTTTTAAGTAAGGTGATCAAAGTTAAGGTACGTATTCCAGGAGAGGtt                                                                                             >  1:42771/1‑149 (MQ=255)
                                                        gTATCCACCTGCGTGGCTACGCACAGAAAGATCCGAAGCAGGAATACAAACGTGAATCGTTCTCCATGTTTGCAGCGATGCTGGAGTCGTTGAAATATGAAGTTATCAGTACGCTGAGCAAAGTTCAGGTACGTATGCCTGAAGAGGtt                                                                                             <  2:1947346/149‑1 (MQ=255)
                                                         tATCCACCTGCGTGGCTACGCACAGAAAGATCCGAAGCAGGAATACAAACGTGAATCGTTCTCCATGTTTGCAGCGATGCTGGAGTCGTTGAAATATGAAGTTATCAGTACGCTGAGCAAAGTTCAGGTACGTATGCCTGAAGAGGTTg                                                                                            <  2:1904770/149‑1 (MQ=255)
                                                         tATCCACCTGCGTGGCTACGCACAGAAAGATCCGAAGCAGGAATACAAACGTGAATCGTTCTCCATGTTTGCAGCGATGCTGGAGTCGTTGAAATATGAAGTTATCAGTACGCTGAGCAAAGTTCAGGTACGTATGCCTGAAGAGGTTg                                                                                            <  2:1668830/149‑1 (MQ=255)
                                                          aTCCACCTGCGTGGCTACGCACAGAAAGATCCGAAGCAGGAATACAAACGTGAATCGTTCTCCATGTTTGCAGCGATGCTGGAGTCGTTGAAATATGAAGTTATCAGTACGCTGAGCAAAGTTCAGGTACGTATGCCTGAAGAGGTTga                                                                                           <  1:3767691/149‑1 (MQ=255)
                                                          aTCCACCTGCGTGGCTACGCACAGAAAGATCCGAAGCAGGAATACAAACGTGAATCGTTCTCCATGTTTGCAGCGATGCTGGAGTCGTTGAAATATGAAGTTATCAGTACGCTGAGCAAAGTTCAGGTACGTATGCCTGAAGAGGTTga                                                                                           <  1:2098164/149‑1 (MQ=255)
                                                             cACCTGCGTGGCTACGCACAGAAAGATCCGAAGCAGGAATACAAACGTGAATCGTTCTCCATGTTTGCAGCGATGCTGGAGTCGTTGTGATATTAAGTTATAAGTTCGCGGTGCAAAGTTCAGGTACGTATGCCGGAAGAGGTTgtggt                                                                                        >  2:3069234/1‑148 (MQ=255)
                                                             cACCTGCGTGGCTACGCACAGAAAGATCCGAAGCAGGAATACAAACGTGAATCGTTCTCCATGTTTGCAGCGATGCTGGAGTCGTTGAAATATGAAGTTATCAGTACGCTGAGCAAAGTTCAGGTACGTATGCCTGAAGAGGTTgagga                                                                                        <  2:2413269/149‑1 (MQ=255)
                                                             cACCTGCGTGGCTACGCACAGAAAGATCCGAAGCAGGAATACAAACGTGAATCGTTCTCCATGTTTGCAGCGATGCTGGAGTCGTGGGAATATGAAGTTATAAGTACGCTGAGCAAAGTTCAGGTACGTATTCCTGAAGAGGTTgtgga                                                                                        >  1:122553/1‑149 (MQ=255)
                                                                 tGCGTGGCTACGCACAGAAAGATCCGAAGCAGGAATACAAACGTGAATCGTTCTCCAGGTTTGCCGCGATGCTGGAGTCGTGGGAAAAGGAAGTTATCACTACGCGGAGCAAAGTTCCGGTACGGATGCCGGGAGAGGTTGGGGGGcag                                                                                    >  1:341071/1‑147 (MQ=255)
                                                                    gTGGCTACGCACAGAAAGATCCGAAGCAGGAATACAAACGTGAATCGTTCTCCATGTTTGCAGCGATGCTGGAGTCGTTGAAATATGAAGTTATCAGTACGCTGAGCAAAGTTCAGGTACGTATGCCTGAAGAGGTTGAGGAGCTGGaa                                                                                 <  1:665885/149‑1 (MQ=255)
                                                                     tGGCTACGCACAGAAAGATCCGAAGCAGGAATACAAACGTGAATCGTTCTCCATGTTTGCAGCGATGCTGGAGTCGTTGAAATATGAAGTTATCAGTACGCTGAGCAAAGTTCAGGTACGTATGCCTGAAGAGGTTGAGGAGCTGGaac                                                                                <  2:3117932/149‑1 (MQ=255)
                                                                         tACGCACAGAAAGATCCGAAGCAGGAATACAAACGTGAATCGTTCTCCATGTTTGCAGCGATGCTGGAGTCGTTGAAATATGAAGTTATCAGTACGCTGAGCAAAGTTCAGGTACGTATGCCTGAAGAGGTTGAGGAGCTGGAacaaca                                                                            <  1:4589034/149‑1 (MQ=255)
                                                                            gCACAGAAAGATCCGAAGCAGGAATACAAACGTGAATCGTTCTCCATGTTTGCAGCGATGCTGGAGTCGTTGAAATATGAAGTTATCAGTACGCTGAGCAAAGTTCAGGTACGTATGCCTGAAGAGGTTGAGGAGCTGGAACAACAGcg                                                                         <  1:1689541/149‑1 (MQ=255)
                                                                             cacaGAAAGATCCGAAGCAGGAATACAAACGTGAATCGTTCTCCATGTTTGCAGCGATGCTGGAGTCGTTGAAATATGAAGTTATCAGTACGCTGAGCAAAGTTCAGGTACGTATGCCTGAAGAGGTTGAGGAGCTGGAACAACAGcgt                                                                        <  1:1449092/149‑1 (MQ=255)
                                                                             cacaGAAAGATCCGAAGCAGGAATACAAACGTGAATCGTTCTCCATGTTTGCAGCGATGCTGGAGTCGTTGAAATATGAAGTTATCAGTACGCTGAGCAAAGTTCAGGTACGTATGCCTGAAGAGGTTGAGGAGCTGGAACAACAGcgt                                                                        <  1:3038167/149‑1 (MQ=255)
                                                                             cacaGAAAGATCCGAAGCAGGAATACAAACGTGAATCGTTCTCCATGTTTGCAGCGATGCTGGAGTCGTGGAAATATGAAGTTATCAGTAAGCTGAGCAAACTTCAGGGAAGTATTCCTGTAGATGTTGAGGAGCAGGTACAACAGcgt                                                                        >  2:1767416/1‑149 (MQ=255)
                                                                              acaGAAAGATCCGAAGCAGGAATACAAACGTGAATCGTTCTCCATGTTTGCAGCGATGCTGGAGTCGTTGAAATATGAAGTTATCAGTACGCTGAGCAAAGTTCAGGTACGTATGCCTGAAGAGGTTGAGGAGCTGGAACAACAGcgtc                                                                       <  2:705138/149‑1 (MQ=255)
                                                                              acaGAAAGATCCGAAGCAGGAATACAAACGTGAATCGTTCTCCATGTTTGCAGCGATGCTGGAGTCGTTGAAATATGAAGTTATCAGTACGCTGAGCAAAGTTCAGGTACGTATGCCTGAAGAGGTTGAGGAGCTGGAACAACAGcgtc                                                                       <  1:1036101/149‑1 (MQ=255)
                                                                               caGAAAGATCCGAAGCAGGAATACAAACGTGAATCGTTCTCCATGTTTGCAGCGATGCTGGAGTCGTTGAAATATGAAGTTATCAGTACGCTGAGCAAAGTTCAGGTACGTATGCCGGAAGAGGTTGAGGAGCTGGAACAACAGcgtcg                                                                      >  2:4329089/1‑149 (MQ=255)
                                                                                aGAAAGATCCGAAGCAGGAATACAAACGTGAATCGTTCTCCATGTTTGCAGCGATGCTGGAGTCGTTGAAATATGAAGTTATCAGTACGCTGAGCAAAGTTCAGGTACGTATGCCTGAAGAGGTTGAGGAGCTGGAACAACAGcgtcgt                                                                     <  1:3294796/149‑1 (MQ=255)
                                                                                 gAAAGATCCGAAGCAGGAATACAAACGTGAATCGTTCTCCATGTTTGCAGCGATGCTGGAGTCGTGGAAATATGAAGTTATCAGTACGCTGAGCAAATTTCAGGTACGTATGCCGGAAGAGGTTGAGGAGCTGGAACAACAGCGTCGTa                                                                    >  2:785122/1‑149 (MQ=255)
                                                                                  aaaGATCCGAAGCAGGAATACAAACGTGAATCGTTCTCCATGTTTGCAGCGATGCTGGAGTCGTTGAAATATGAAGTTATCAGTACGCTGAGCAAAGTTCAGGTACGTATGCCTGAAGAGGTTGAGGAGCTGGAACAACAGCGTCGTAt                                                                   <  2:1363174/149‑1 (MQ=255)
                                                                                    aGATCCGAAGCAGGAATACAAACGTGAATCGTTCTCCATGTTTGCAGCGATGCTGGAGTCGTTGAAATATGAAGTTATCAGTACGCTGAGCAAAGTTCAGGTACGTATGCCTGAAGAGGTTGAGGAGCTGGAACAACAGCGTCGTATgg                                                                 <  1:4814622/149‑1 (MQ=255)
                                                                                      aTCCGAAGCAGGAATACAAACGTGAATCGTTCTCCATGTTTGCAGCGATGCTGGAGTCGTTGAAATATGAAGTTATCAGTACGCAGAGCAAAGTTCAGGTACGTATGCCAGAAGAGGTTGAGGAGCTGGAACAACAGCGTCGTATGGaa                                                               >  2:1311360/1‑149 (MQ=255)
                                                                                       tCCGAAGCAGGAATACAAACGTGAATCGTTCTCCATGTTTGCAGCGATGCTGGAGTCGTGGGAATATGAAGTTATCAGTAAGCTGGGCCAAATTCAGGGACGTGTGCCGGTAGAGGTTGTGGCGCGGGAACAACAACGTCGGATGGAAg                                                              >  1:3522173/1‑149 (MQ=255)
                                                                                        ccGAAGCAGGAATACAAACGTGAATCGTTCTCCATGTTTGCAGCGATGCTGGAGTCGTTGAAATATGAAGTTATAAGTAAGCTGAGCAAAGTTAAGGTACGTATGCCTGAAGAGGTTGAGGAGCTGGAACAACAGCGTCGTATGGAAGc                                                             >  2:1204016/1‑149 (MQ=255)
                                                                                           aaGCAGGAATACAAACGTGAATCGTTCTCCATGTTTGCAGCGATGCTGGAGTCGTTGAAATATGAAGTTATCAGTACGCTGAGCAAAGTTCAGGTACGTATGCCTGAAGAGGTTGAGGAGCTGGAACAACAGCGTCGTATGGAAGCCGa                                                          <  2:4808001/149‑1 (MQ=255)
                                                                                            aGCAGGAATACAAACGTGAATCGTTCTCCATGTTTGCAGCGATGCTGGAGTCGTTGAAATATGAAGTTATCAGTACGCTGAGCAAAGTTAAGGTACGTATTCCGGAAGAGGTTGAGGAGCTGGAACAACAGCGTCGTATGGAAGCCGAg                                                         >  2:537360/1‑149 (MQ=255)
                                                                                             gCAGGAATACAAACGTGAATCGTTCTCCCTGTTTGCAGCGATGCTGGAGTCGTTGAAATATGAAGTTATCAGTACGCTGAGCAAAGTTCAGGTACGTATGCCTGAAGAGGTTGAGGAGCTGGAACAACAGCGTCGTATGGAAGCCGAGc                                                        <  2:4838669/149‑1 (MQ=255)
                                                                                               aGGAATACAAACGTGAATCGTTCTCCATGTTTGCAGCGATGCTGGAGTCGTTGAAATATGAAGTTATCAGTACGCTGAGCAAAGTTCAGGTACGTATGCCTGAAGAGGTTGAGGAGCTGGAACAACAGCGTCGTATGGAAGCCGAGCGt                                                      <  1:3934671/149‑1 (MQ=255)
                                                                                                ggAATACAAACGTGAATCGTTCTCCATGTTTGCAGCGATGCTGGAGTCGTTGAAATATGAAGTTATCAGTACGCTGAGCAAAGGTCAGGTACGTATGCCTGAAGAGGTTGAGGAGCTGGAACAACAGCGGCGTATTGAAGCCGAGCGtt                                                     >  2:3303237/1‑149 (MQ=255)
                                                                                                ggAATACAAACGTGAATCGTTCTCCATGTTTGCAGCGATGCTGGAGTCGTTGAAATATGAAGTTATCAGTACGCGGAGCAAAGTTCAGGTACGTATGCCTGAAGAGGTTGAGGAGCTGGAACAACAGCGGCGTATGGAAGCCGAGCGtt                                                     >  2:1163591/1‑149 (MQ=255)
                                                                                                 gAATACAAACGTGAATCGTTCTCCATGTTTGCAGCGATGCTGGAGTCGTTGAAATATGAAGTTATCAGTACGCTGAGCAAAGTTCAGGTACGTATGCCTGAAGAGGTTGAGGAGCTGGAACAACAGCGTCGTATGGAAGCCGAGCGttt                                                    <  1:4339108/149‑1 (MQ=255)
                                                                                                  aaTACAAACGTGAATCGTTCTCCATGTTTGCAGCGATGCTGGAGTCGTTGAAATATGAAGTTATCAGTACGCAGAGCAAAGTTCAGGTACGTATTACTGAAGAGGTTGAGGAGCTGGAACAACAGCGTCGTATGGAAGCCGAGCGTTTa                                                   >  2:749614/1‑149 (MQ=255)
                                                                                                    tACAAACGTGAATCGTTCTCCATGTTTGCAGCGATGCTGGAGTCGTTGAAATATGAAGTTATCAGTACGCTGAGCAAAGTTCAGGTACGTATGCCTGAAGAGGTTGAGGAGCTGGAACAACAGCGTCGTATGGAAGCCGAGCGTTTAgc                                                 <  1:1627010/149‑1 (MQ=255)
                                                                                                       aaaCGTGAATCGTTCTCCATGTTTGCAGCGATGCTGGAGTCGTTGTAATATGAAGTTATCAGTACGCTGAGCAAAGTTAAGGTAAGTGTTCCTGAAGGGGGTGTGGGGCTGGTACCACAGCGTCGGATTGGAGCCGAGCGGTTTGCGCa                                              >  2:3480986/1‑149 (MQ=255)
                                                                                                       aaaCGTGAATCGTTCTCCATGTTTGCAGCGATGCTGGAGTCGTTGAAATATGAAGTTATCAGTACGCTGAGCAAAGTTCAGGTACGTATGCCTGAAGAGGTTGAGGAGCTGGAACAACAGCGTCGTATGGAAGCCGAGCGTTTAGCGCa                                              <  1:2350295/149‑1 (MQ=255)
                                                                                                        aaCGTGAATCGTTCTCCATGTTTGCAGCGATGCTGGAGTCGTTGAAATATGAAGTTATCAGTACGCTGAGCAAAGTTCAGGTACGTATGCCTGAAGAGGTTGAGGAGCTGGAACAACAGCGTCGTATGGAAGCCGAGCGTTTAGCGCaa                                             <  1:2272477/149‑1 (MQ=255)
                                                                                                        aaCGTGAATCGTTCTCCATGTTTGCAGCGATGCTGGAGTCGTGGTTATATGAATTTATAACTACGCGGAGCAAAGTTAAGGTACGGTTGCCGGTAGTGGTGGTGGAGCTGGAACAACAGCGTCGTCTGGAAGCCCAGCGTTTAGCGCac                                             >  1:1049334/1‑148 (MQ=255)
                                                                                                         aCGTGAATCGTTCTCCATGTTTGCAGCGATGCTGGAGTCGTTGAAATATGAAGTTATCAGTACGCTGAGCAAAGTTCAGGTACGTATGCCTGAAGAGGTTGAGGAGCTGGAACAACAGCGTCGTATGGAAGCCGAGCGTTTAGCGCaaa                                            <  1:1436759/149‑1 (MQ=255)
                                                                                                         aCGTGAATCGTTCTCCATGTTTGCAGCGATGCTGGAGTCGTTGAAATATGAAGTTATCAGTACGCTGAGCAAAGTTCAGGTACGTATGCCTGAAGAGGTTGAGGAGCTGGAACAACAGCGTCGTATGGAAGCCGAGCGTTTAGCGCaaa                                            <  2:2976301/149‑1 (MQ=255)
                                                                                                          cGTGAATCGTTCTCCATGTTTGCAGCGATGCTGGAGTCGTTGAAATATGAAGTTATCAGTACGCTGAGCAAAGTTCAGGTACGTATGCCTGAAGAGGTTGAGGAGCTGGAACAACAGCGTCGTATGGAAGCCGAGCGTTTAGCGCAAAt                                           <  2:3271711/149‑1 (MQ=255)
                                                                                                             gAATCGTTCTCCATGTTTGCAGCGATGCTGGAGTCGTGGGAATAAGAAGTTATCAGTACGCGGAGCAAAGTTAAGGTACGTAAGCCTGAAGAGGTTGAGGAGCTGGAACAACACCCTCGGATGGAAGCCCAGCGGTTAGCGCAAATgca                                        >  1:2874384/1‑149 (MQ=255)
                                                                                                              aaTCGTTCTCCATGTTTGCAGCGATGCTGGAGTCGTTGTAATATGAAGTTATAAGTAAGCTGATCAAAGTTAAGGTAAGTATTCCTGAAGAGGTTGAGGAGCTTGAAAAAAAGCGTAGTATGGAATCCGAGCGTTTATCGCAAATtcag                                       >  2:3850489/1‑149 (MQ=255)
                                                                                                               aTCGTTCTCCATGTTTGCAGCGATGCTGGAGTCGTTGAAATATGAAGTTATCAGTACGCTGAGCCAAGTTCAGGTACGTATGCCTGAAGAGGTTGTGGAGCTGGAACAACAGCGTCGTATGGAAGCCGAGCGTTTAGCGCAAATgcagc                                      >  2:2863533/1‑149 (MQ=255)
                                                                                                               aTCGTTCTCCATGTTTGCAGCGATGCTGGAGTCGTGGTAATATGTAGTTATAAGTACGCTGAGCCAAGTTCAGGTAAGTATGCCTGAAGAGGGTGAGGAGCTGGAACAACAGCGTCGTATGGTAGCCGAGCGTTTAGCGCAAATgcagc                                      >  2:368381/1‑149 (MQ=255)
                                                                                                                    tctcCATGTTTGCAGCGATGCTGGAGTCGTGGTAAAATGAAGTTATAAGTAAGCTGAGCAAAGTTCAGGTACGTATGCCTGAAGAGGTTGAGGAGCTGGAAAAACAGCGTCGTATGGAAGCCGAGCGTTTAGCGCAAATGCaccagcgt                                 >  1:5176425/1‑147 (MQ=255)
                                                                                                                       ccATGTTTGCAGCGATGCTGGAGTCGTTGAAATATGAAGTTATCAGTACGCTGAGCAAAGTTCAGGTACGTATGCCTGAAGAGGTTGAGGAGCTGGAACAACAGCGTCGTATGGAAGCCGAGCGTTTAGCGCAAATGCAGCAGCTTAGc                              <  2:3586402/149‑1 (MQ=255)
                                                                                                                       ccATGTTTGCAGCGATGCTGGAGTCGTTGAAATATGAAGTTATCAGTACGCTGAGCAAAGTTCAGGTACGTATGCCTGAAGAGGTTGAGGAGCTGGAACAACAGCGTCGTATGGAAGCCGAGCGTTTAGCGCAAATGCAGCAGCTTAGc                              <  2:950204/149‑1 (MQ=255)
                                                                                                                         aTGTTTGCAGCGATGCTGGAGTCGTTGAAATATGAAGTTATCAGTACGCTGAGCAAAGTTCAGGTACGTATGCCTGAAGAGGTTGAGGAGCTGGAACAACAGCGTCGTATGGAAGCCGAGCGTTTAGCGCAAATGCAGCAGCTTAGCCa                            <  2:1018163/149‑1 (MQ=255)
                                                                                                                          tGTTTGCAGCGATGCTGGAGTCGTTGAAATATGAAGTTATCAGTACGCTGAGCAAAGTTCAGGTACGTATGCCTGAAGAGGTTGAGGAGCTGGAACAACAGCGTCGTATGGAAGCCGAGCGTTTAGCGCAAATGCAGCAGCTTAGCCAt                           <  1:2815414/149‑1 (MQ=255)
                                                                                                                          tGTTTGCAGCGATGCTGGAGTCGTTGAAATATGAAGTTATCAGTACGCTGAGCAAAGTTCAGGTACGTATGCCTGAAGAGGTTGAGGAGCTGGAACAACAGCGTCGTATGGAAGCCGAGCGTTTAGCGCAAATGCAGCAGCTTAGCCAt                           <  1:1099233/149‑1 (MQ=255)
                                                                                                                               gCAGCGATGCTGGAGTCGTTGAAATATGAAGTTATCAGTACGCTGAGCAAAGTTCAGGTACGTATGCCTGAAGAGGTTGAGGAGCTGGAACAACAGCGTCGTATGGAAGCCGAGCGTTTAGCGCAAATGCAGCAGCTTAGCCATCAGGa                      <  2:3081046/149‑1 (MQ=255)
                                                                                                                                   cGATGCTGGAGTCGTTGAAATATGAAGTTATCAGTACGCTGAGCAAAGTTCAGGTACGTATGCCTGAAGAGGTTGAGGAGCTGGAACAACAGCGTCGTATGGAAGCCGAGCGTTTAGCGCAAATGCAGCAGCTTAGCCATCAGGATgac                  <  1:2932268/149‑1 (MQ=255)
                                                                                                                                        cTGGAGTCTTTGAAATATGAATTTATCAGTACGCTGAGCAAAGTTCTGGTACGTATGCCTGAAGAGGTTGAGGAGCTGGAACAACAGCGTCGTATGGAAGCCGAGCGTTTAGCGCAAATGCAGCAGCTTAGCAATCAGGATGACGActc             <  2:461992/149‑1 (MQ=255)
                                                                                                                                            aGTCGTTGAAATATGAAGTTATCAGTACGCTGAGCAAAGTTCAGGTACGTATGCCTGAAGAGGTTGAGGAGCTGGAACAACAGCGTCGTATGGAAGCCGAGCGTTTAGCGCAAATGCAGCAGCTTAGCCATCAGGATGACGACTCTGCa         <  1:1982415/149‑1 (MQ=255)
                                                                                                                                              tCGTTGAAATATGAAGTTATCAGTACGCTGAGCAAAGTTCAGGTACGTATGCCTGAAGAGGTTGAGGAGCTGGAACAACAGCGTCGTATGGAAGCCGAGCGTTTAGCGCAAATGCAGCAGCTTAGCCATCAGGATGACGACTCTGCAGc       >  1:5091901/1‑149 (MQ=255)
                                                                                                                                                 ttGAAATATGAAGTTATCAGTACGCTGAGCAAAGTTCAGGTACGTATGCCTGAAGAGGTTGAGGAGCTGGAACAACAGCGTCGTATGGAAGCCGAGCGTTTAGCGCAAATGCAGCAGCTTAGCCATCAGGATGACTACTCTGCAGCCGc    >  2:4135086/1‑149 (MQ=255)
                                                                                                                                                 ttGAAATATGAAGTTATCAGTACGCTGAGCAAAGTTCAGGTACGTATGCCTGAAGAGGTTGAGGAGCTGGAACAACAGCGTCGTATGGAAGCCGAGCGTTTAGCGCAAATGCAGCAGCTTAGCCATCAGGATGACGACTCTGCAGCCGc    >  2:4610169/1‑149 (MQ=255)
                                                                                                                                                 gtGAAATATGAAGTTATCAGTACGCTGAGCAAAGTTCAGGTACGTATGCCTGAAGAGGTTGAGGAGCTGGAACAACAGCGTCGTATGGAAGCCGAGCGTTTAGCGCAAATGCAGCAGCTTAGCCATCAGGATGACGACTCTGCAGCCGc    >  2:2539998/2‑149 (MQ=255)
                                                                                                                                                   gAAATATGAAGTTATCAGTACGCTGAGCAAAGTTCAGGTACGTATGCCTGAAGAGGTTGAGGAGCTGGAACAACAGCGTCGTATGGAAGCCGAGCGTTTAGCGCAAATGCAGCAGCTTAGCCATCAGGATGACGACTCTGCAGCCGCAg  >  1:753471/1‑149 (MQ=255)
                                                                                                                                                    |                                                                                                                                                   
AACGCTTGACTCCCTGTGGAAAGAGCACCTGGCAGCGATGGACTATCTGCGTCAGGGTATCCACCTGCGTGGCTACGCACAGAAAGATCCGAAGCAGGAATACAAACGTGAATCGTTCTCCATGTTTGCAGCGATGCTGGAGTCGTTGAAATATGAAGTTATCAGTACGCTGAGCAAAGTTCAGGTACGTATGCCTGAAGAGGTTGAGGAGCTGGAACAACAGCGTCGTATGGAAGCCGAGCGTTTAGCGCAAATGCAGCAGCTTAGCCATCAGGATGACGACTCTGCAGCCGCAG  >  CP009273/107072‑107367

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑