BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	1,282,605	G→A	6.2%	noncoding (88/171 nt) R10* (CGA→TGA)	rttR ← tpr ←	rtT sRNA, processed from tyrT transcript protamine‑like protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	1,282,605	0	G	A	6.2%	343.3 / 8.6	119	noncoding (88/171 nt) R10* (CGA→TGA)	rttR tpr	rtT sRNA, processed from tyrT transcript protamine‑like protein
Reads supporting (aligned to +/- strand): ref base G (45/63); new base A (4/3); total (51/68)
Fisher's exact test for biased strand distribution p-value = 4.57e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 123 total aligned reads displayed.

TTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGATTTACAGT  >  CP009273/1282457‑1282753

ttCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCAGCGGCAACCCACACACGCCGGcgcgcg                                                                                                                                                      >  1:4322179/1‑149 (MQ=14)

 tCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTGTCCTGGGGCAAAGCAAGCAAGCTGGCGCGCGa                                                                                                                                                     >  1:1138179/1‑149 (MQ=14)

    ccGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCGGCGGCCACCCCCACTCCCTGGcgcgcgcaga                                                                                                                                                  >  1:2759497/1‑145 (MQ=14)

    ccGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTGGCAGTCGTCCACTCTCTCTCGCTGGCGCGCGACTc                                                                                                                                                  >  2:663001/1‑149 (MQ=14)

    ccGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTAGTTGCCTGCGGCAACGCTCTCTAGCTGGCGCTCGAGta                                                                                                                                                  >  2:661170/1‑148 (MQ=255)

        aTGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGAGGCAAACCACTCTCGCTGGCGCGCGAGTCCaaa                                                                                                                                              >  2:2300379/1‑148 (MQ=14)

         tGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAAcc                                                                                                                                             >  2:2306899/1‑149 (MQ=255)

         tGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTGTCCGGCGGCAAACCCCTCTCGCGGGCGCGCGAGTCGAAcc                                                                                                                                             >  1:4295541/1‑149 (MQ=14)

            tcAGAATATTTGATTGCGGATTCGATTGAGATTTCAGGGTCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTg                                                                                                                                          <  1:429305/148‑1 (MQ=255)

            gCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCCACGCACCCACGCTGGCGCGCGAGTCGaaacaag                                                                                                                                          >  1:4917006/1‑144 (MQ=14)

             cAGAATTTTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTgg                                                                                                                                         <  2:499507/149‑1 (MQ=255)

                 atatTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCAGAGGCAAACCACAAACGCTAGCGCTCCAGGCGAAACATGGTCGa                                                                                                                                     >  2:3943743/1‑149 (MQ=14)

                   atTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTTCCAGCGGCAACCCTCACACGCTGGCGCACGAGTCGAACCATGGTCGAAg                                                                                                                                   >  1:3852614/1‑149 (MQ=14)

                   atTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAg                                                                                                                                   <  1:4399505/149‑1 (MQ=255)

                    tttGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTTGCCCTCGAGTCGAACCTTTGTCGaatc                                                                                                                                  >  2:2063263/1‑147 (MQ=255)

                    tttGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAAAGCACACTCGCTTGCGCTAGAGTCGAAACTTGGTAGaata                                                                                                                                  >  2:2063257/1‑147 (MQ=14)

                     ttGATTGCGGATTCGATTGAGAGTTCAGGGTCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCt                                                                                                                                 <  1:1357064/149‑1 (MQ=255)

                       gATTGCGGATTCGTTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTtc                                                                                                                               <  2:3578688/149‑1 (MQ=255)

                       gATTGCGGATTCGGTTGAGAGTTCCGGGTCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTtc                                                                                                                               <  2:3578614/149‑1 (MQ=255)

                       gATTGCGGATTCGCTTGTGAGTTCAGGGTCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTtc                                                                                                                               <  1:2778329/149‑1 (MQ=255)

                       gATTGCGGATTCGATTGAGAGTTCCGGGTCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTtc                                                                                                                               <  2:3578690/149‑1 (MQ=255)

                         ttGCGGATTCGATTGAGATTTCCGGGTCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTtctc                                                                                                                             <  1:1659774/149‑1 (MQ=255)

                             ggATTCGTTTGAGACTTCAGGGTCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATcc                                                                                                                         <  2:3249427/149‑1 (MQ=255)

                              gATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGGTGCCGGAGGCCAACCACTCTCGCTGGCGCGAGAGGGGGAACGTGGTCGAAGCTTATCAAcca                                                                                                                        >  2:1493579/1‑148 (MQ=14)

                                 tCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTcc                                                                                                                     <  1:3094880/149‑1 (MQ=255)

                                  cgattGAGAGTTCAGGGTCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTccc                                                                                                                    <  2:3008642/146‑1 (MQ=255)

                                  cGCTTGAGAGTTCCGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTccc                                                                                                                    <  2:3008831/149‑1 (MQ=255)

                                         gagTTCAGGGTCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATgg                                                                                                             <  2:4935553/149‑1 (MQ=255)

                                         gagTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATgg                                                                                                             <  1:1125129/149‑1 (MQ=255)

                                          agTACAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATggg                                                                                                            <  1:1929605/149‑1 (MQ=255)

                                           gTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGc                                                                                                           <  2:3158821/149‑1 (MQ=255)

                                             tCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCAGCGGCAACGCGCCCTCGCGGGCCCTCGAGTCCCACCGTGGGAGAAGCTAAACATCCTACCCCGCAACGGCaa                                                                                                         >  2:2881539/1‑148 (MQ=14)

                                                gggTCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAat                                                                                                      <  1:2085771/149‑1 (MQ=37)

                                                gggACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTATCTCGCTTGCGCTCGAGTCGAACCATGGTCGAAGCATCTCAAACATCCCCCCAAGGGCCGaaa                                                                                                      >  2:4279867/1‑148 (MQ=14)

                                                            aGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCACTCTAGCTGGCGCACGAGTCGAACCATGGTCGAAGCTTCTCAACCTTCCCCCCAAGGGCAGAATATTTAATTGCgg                                                                                          >  2:797746/1‑149 (MQ=14)

                                                            aGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCAGCGGCAACGCTCACTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCAACCTTCACCCCAAGGGCAGAATATTTAATTGCgg                                                                                          >  2:797749/1‑149 (MQ=14)

                                                             gTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCACGCTGGCGCGCGAGTCGAACCTAGGTCGAAGCATAACATCCATACCCGCCAGGGCAGAATATTTAAtttccga                                                                                         >  2:5154729/1‑144 (MQ=11)

                                                                          gggAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCTCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGt                                                                            <  1:1014781/149‑1 (MQ=255)

                                                                          gggAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGt                                                                            <  1:2303840/149‑1 (MQ=255)

                                                                           ggAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCGTGGTGGAAGCGTCTCATCCCTCCCCGCATGGGCAGAAGATTTAATTTCGGATTAGTTGGGAAGtt                                                                           >  2:2135431/1‑149 (MQ=14)

                                                                             aaGGAGTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCa                                                                         <  1:1328413/149‑1 (MQ=255)

                                                                              aGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATACTTCCCCGCATGGGCAGAATATTTAATTGCGGGTTAGTTGGGGAGTTCAg                                                                        >  2:4235157/1‑149 (MQ=255)

                                                                               ggATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCGCTAACGCTGGCGCGCGAGTCGAACCGTGGTCGGAGCGTATAAAACATCACCCCCAGGGCAGAAAATATAATTTCGGATTAGGTGGGTAGGTCAgg                                                                       >  2:3686678/1‑149 (MQ=2)

                                                                                gATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAggg                                                                      <  1:4991888/149‑1 (MQ=255)

                                                                                 aTTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGTCGCTCGAGTCGAACCTTCGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTTATTGCGGATTCGTTGGGAAGTTCAGGGa                                                                     <  1:3024697/149‑1 (MQ=255)

                                                                                 aTTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGa                                                                     <  1:3024741/149‑1 (MQ=255)

                                                                                  ttACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGTCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGAc                                                                    <  1:4195623/149‑1 (MQ=255)

                                                                                  ttACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTaa                                                                                                >  2:3895176/1‑121 (MQ=37)

                                                                                   tACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCTAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACt                                                                   <  1:843959/149‑1 (MQ=255)

                                                                                   tACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACt                                                                   <  1:1056089/149‑1 (MQ=255)

                                                                                   tACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACt                                                                   <  1:4715135/149‑1 (MQ=255)

                                                                                        aGCGCTGCGCGCTTCGCCCTTCGGGTCGTGGGCAGCGGCCACCCTCAATCGCTGGGGCGCGAGTCGAACCGGGGTCGAAGCGTCACAACCGTGCCCGCCGGGGGAGAAAAGTTAATTTCGGCTTGGTTGGGGAGTTCAGGGCCGTTTgg                                                              >  2:3102983/1‑148 (MQ=9)

                                                                                          cgcTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGaaa                                                            <  2:4162976/149‑1 (MQ=255)

                                                                                              gcgcgcTTCGCCCTTCGGGTCGGTGCCTGCGGCTACGCTCTCTCGCTGGCGCTCGAGTCGAACCGTGGTCTAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGa                                                        <  1:3938057/149‑1 (MQ=255)

                                                                                                  gcTTCGCCCTTCGGGTCGTTGCCTGCGGCCACGCTCTCTCGCTGGCGCTCGAGGCGTACCTTTGTCGAATCTTCGAATACATCCCCCCCAGGGGCGAATATTTAATTGGGGGTTAGGTGGGGAGTTAAGGGACGTTTTTAAGTGAttgt                                                    >  2:4285182/1‑149 (MQ=2)

                                                                                                      cGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGAtggtggtg                                                <  2:2392611/149‑1 (MQ=255)

                                                                                                                gTCGTTGCCTGCGGCAACGCTCTCTCGCTGTCGCTCGAGCCGAACCTTTGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGAt                                      <  1:2319095/149‑1 (MQ=255)

                                                                                                                 tCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGAtt                                     <  2:326996/149‑1 (MQ=255)

                                                                                                                     tGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATggg                                                                                                            >  2:2028377/1‑74 (MQ=39)

                                                                                                                     tGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATggg                                                                                                            <  1:2028377/74‑1 (MQ=39)

                                                                                                                     tGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGCACCTTCGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGaa                                 <  2:4384474/149‑1 (MQ=255)

                                                                                                                      gCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAAc                                >  2:4519569/1‑149 (MQ=255)

                                                                                                                      gCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAAc                                >  2:3854209/1‑149 (MQ=255)

                                                                                                                      gCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAAc                                >  2:4521873/1‑149 (MQ=255)

                                                                                                                      gCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCGTGGTCGAAGCTTGTCAACCATCCCCGCATGGGCAGAAAATTTAATTTCGGATTCGTTGGGAAGTTCAGGCACTTTTGAAATTGTTGGGGGTGGGGGGCGGATTAGaag                                >  1:3577634/1‑148 (MQ=17)

                                                                                                                        cTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTTGGAAGTTCAGGGACTTTTTAAAGTGATGGTGGTGGGGGAAGGATTAGAAcca                              >  1:2316138/1‑148 (MQ=255)

                                                                                                                        cTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATACTTCCCCGCAGGGGCAGAATATTTAATTGCGGATTAGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGGAGGATTCGaaaca                              >  1:1047448/1‑146 (MQ=17)

                                                                                                                         tGCGGCTTCGCTCTCTCGCGGTCGCTCGAGTCGAACCGCGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTTATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAACCtt                             <  1:4481910/149‑1 (MQ=17)

                                                                                                                         tGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTGGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAACCtt                             <  1:4481899/149‑1 (MQ=255)

                                                                                                                          gCGGCAACGCTCTCTCGCTGTCGCTCGAGCCGAACCTTTGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTTATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAACCTTc                            <  1:530640/149‑1 (MQ=255)

                                                                                                                          gCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAACCTTc                            <  1:2532153/149‑1 (MQ=255)

                                                                                                                          gCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAACCTTc                            <  1:715539/149‑1 (MQ=255)

                                                                                                                           cGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAACCTTCg                           <  1:4025645/149‑1 (MQ=255)

                                                                                                                             gCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTATCATCCTTACCCGCAAGGGCAGAATATTTAATTGCGGATTGGTTGGGTAGGTCATGGAGTTTTGTAATGGATGGTGGGGGGGGAAGGATTAGCACCTTCGaa                         >  2:1732922/1‑149 (MQ=17)

                                                                                                                               aaCGCTCTCTCGCTGGCGCTCAAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGt                                                                                    <  2:2584329/88‑1 (MQ=18)

                                                                                                                                  gCTCTCTCGCTGGCGCTCGAGTCGAACCTTTGTCGAATCTTCTCAAACTTCCCCGCATGGGCAGAATATTTAAATGCGGATGCGTTGGGACATTCAGGGCCGTTTGATAGTGATGGTGGGGGGGGTAGGATTCGAAACGTCGAAGACGa                    >  2:4830906/1‑149 (MQ=17)

                                                                                                                                  gCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGa                    >  2:2869301/1‑149 (MQ=255)

                                                                                                                                  gCTCTCTCGCTGGCGCTCGAGTCGAACCGTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGa                    <  2:2333232/149‑1 (MQ=255)

                                                                                                                                  gCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGaa                                          >  1:2581772/1‑127 (MQ=255)

                                                                                                                                  gCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGaa                                          <  2:2581772/127‑1 (MQ=255)

                                                                                                                                   ctctctCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGAt                   <  1:4285182/149‑1 (MQ=255)

                                                                                                                                    tctctcGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTATCATACTTCCCCGCATGGGCAGAATATTTAAATGCGGATTCGTTTGGAAGTTCAGGGACGTTTGAAAGTGATGGTGGTGGGGGACGGATTAGAACCATCGAAGTCGATg                  >  1:3111450/1‑149 (MQ=17)

                                                                                                                                     ctctcGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGa                 <  1:3745858/149‑1 (MQ=255)

                                                                                                                                     ctctcGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGa                 <  1:3198880/149‑1 (MQ=255)

                                                                                                                                     ctctcGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCAACCTTCCCCGCATGGGCAGAATATTTAATTGCGGAGTCGTGGGGAAGTTCAGGGTCTTTTGAAAGTGATGGTGGTGGGGGAAGGCTTATATCCTTAGAAGACGaagt                 >  1:4302549/1‑146 (MQ=17)

                                                                                                                                      tctcGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGAc                <  1:2159766/149‑1 (MQ=255)

                                                                                                                                        tcGCTGGCGCTCAAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCa                                                                         <  2:1714477/90‑1 (MQ=255)

                                                                                                                                        tcGCTGGCGCTCAAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCa                                                                         >  1:1714477/1‑90 (MQ=255)

                                                                                                                                         cGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGGc             >  2:1573972/1‑149 (MQ=255)

                                                                                                                                           cTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAg           >  1:4697299/1‑149 (MQ=255)

                                                                                                                                           cTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGGTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAg           >  2:4911853/1‑149 (MQ=255)

                                                                                                                                             ggagcTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGAt         <  1:4554150/146‑1 (MQ=255)

                                                                                                                                             gacgcTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGAt                                                                                        <  1:2790507/68‑1 (MQ=255)

                                                                                                                                              gcgcTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGAtt        <  1:3406237/149‑1 (MQ=255)

                                                                                                                                              gcgcTCGAGTCGAACCGTGGTCGAAGCTTCTCTTCCTGCCCCGCATGGGCTGAATATTTATTTGCGGATTCGTTGGGTAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGAtt        <  1:4597318/149‑1 (MQ=21)

                                                                                                                                              ccgcTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGAtt        <  1:608743/148‑1 (MQ=255)

                                                                                                                                                gcTCAAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTaa                                                                                                <  2:4834455/59‑1 (MQ=16)

                                                                                                                                                gcTCAAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTaa                                                                                                >  1:4834455/1‑59 (MQ=16)

                                                                                                                                                 cTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGATTTAc     <  2:2299049/149‑1 (MQ=255)

                                                                                                                                                    gAGTCGAACCTTGGTCGAAGCTTCACATCCGTCCCCGCATGGGCAGAAAATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGTTAGTGATGGTGGTGGGGGGTGGATTCGAACCTTCGAAGTCGATGTCGGCGGATTTATAGt  >  1:2634010/1‑149 (MQ=21)

TTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGATTTACAGT  >  CP009273/1282457‑1282753

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`