Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,673,380 T→A 7.3% S233T (TCA→ACA) ‡ ydgH → DUF1471 family periplasmic protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,673,3800TA 7.3% 690.5 / 11.5 226S233T (TCA→ACA) ‡ydgHDUF1471 family periplasmic protein
Reads supporting (aligned to +/- strand):  ref base T (83/122);  new base A (6/14);  total (90/136)
Fisher's exact test for biased strand distribution p-value = 4.74e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 235 total aligned reads displayed.

GAGCCCGGATGTGATCCCGGCAGATTCCGAAGCAGGACGTGCAGCTCTGGCTGCCGGTGGCGAAGCCGCGAAGAAAGTTGAGATCCCGGGTGTTGCGACTACCGCATCACCAAGTTCTGAAGTCGGTCGCTTCTTTGAAACCCAGTCATCAAAAGGCGGGCGTTACACCGTCACGCTCCCGGATGGCACTAAAGTCGAAGAACTGAACAAAGCGACCGCAGCGATGATGGTCCCGTTCGACAGCATCAAATTCTCTGGCAACTACGGCAACATGACCGAAGTCTCCTATCAGGTTGC  >  CP009273/1673232‑1673528
                                                                                                                                                    |                                                                                                                                                    
 agCCCGGATGTGATCCCGGCAGATTCCGAAGCAGGACGTGCAGCTCTGGCTGCCGGTGGCGAAGCCGCGAAGAAAGTTGAGATCCCGGGTGTTGCGACTACCGCATCACCAAGTTCTGAAGTCGGTCGCTTCTTTGAAACCCAGtcatc                                                                                                                                                     <  2:2646462/149‑1 (MQ=255)
     cGGATGTGATCCCGGCAGATTCCGAAGCAGGACGTGCAGCTCTGGCTGCCGGTGGCGAAGCCGCGAAGAAAGTTGAGATCCCGGGTGTTGCGACTACCGCATCACCAAGTTCTGAAGTCGGTCGCTTCTTTGAAACCCAGTCATCaaaa                                                                                                                                                 >  2:2484274/1‑149 (MQ=255)
      ggATGTGATCCCGGCAGATTCCGAAGCAGGACGTGCAGCTCTGGCTGCCGGTGGCGAAGCCGCGAAGAAAGTTGAGATCCCGGGTGTTGCGACTACCGCATCACCAAGTTCTGAAGTCGGTCGCTTCTTTGAAACCCAGTCATCAAAAg                                                                                                                                                <  2:1433065/149‑1 (MQ=255)
        aTGTGATCCCGGCAGATTCCGAAGCAGGACGTGCAGCTCTGGCTGCCGGTGGCGAAGCCGCGAAGAAAGTTGAGATCCCGGGTGTTGCGACTACCGCATCACCAAGTTCTGAAGTCGGTCGCTTCTTTGAAACCCAGTCATCAAAAggc                                                                                                                                              <  1:1534495/149‑1 (MQ=255)
         tgtgATCCCGGCAGATTCCGAAGCAGGACGTGCAGCTCTGGCTGCCGGTGGCGAAGCCGCGAAGAAAGTTGAGATCCCGGGTGTTGCGACTACCGCATCACCAAGTTCTGAAGTCGGTCGCTTCTTTGAAACCCAGTCATCAAAAggcg                                                                                                                                             <  1:485875/149‑1 (MQ=255)
          gtgATCCCGGCAGATTCCGAAGCAGGACGTGCAGCTCTGGCTGCCGGTGGCGAAGCCGCGAAGAAAGTTGAGATCCCGGGTGTTGCGACTACCGCATCACCAAGTTCTGAAGTCGGTCGCTTCTTTGAAACCCAGTCATCAAAAggcgg                                                                                                                                            <  2:522087/149‑1 (MQ=255)
             aTCCCGGCAGATTCCGAAGCAGGACGTGCAGCTCTGGCTGCCGGTGGCGAAGCCGCGAAGAAAGTTGAGATCCCGGGTGTTGCGACTACCGCATCACCAAGTTCTGAAGTCGGTCGCTTCTTTGAAACCCAGTCATCAAAAggcgggcg                                                                                                                                         <  2:3599354/149‑1 (MQ=255)
             aTCCCGGCAGATTCCGAAGCAGGACGTGCAGCTCTGGCTGCCGGTGGCGAAGCCGCGAAGAAAGTTGAGATCCCGGGTGTTGCGACTACCGCATCACCAAGTTCTGAAGTCGGTCGCTTCTTTGAAACCCAGTCATCAAAAggcgggcg                                                                                                                                         <  2:3702290/149‑1 (MQ=255)
              tCCCGGCAGATTCCGAAGCAGGACGTGCAGCTCTGGCTGCCGGTGGCGAAGCCGCGAAGAAAGTTGAGATCCCGGGTGTTGCGACTACCGCATCACCAAGTTCTGAAGTCGGTCGCTTCTTTGAAACCCAGTCATCAAAAGGCGGGCGt                                                                                                                                        >  2:2518215/1‑149 (MQ=255)
                ccGGCAGATTCCGAAGCAGGACGTGCAGCTCTGGCTGCCGGTGGCGAAGCCGCGAAGAAAGTTGAGATCCCGGGTGTTGCGACTACCGCATCACCAAGTTCTGAAGTCGGTCGCTTCTTTGAAACCCAGTCATCAAAAGGCGGGCGTTa                                                                                                                                      <  1:935703/149‑1 (MQ=255)
                   gCAGATTCCGAAGCAGGACGTGCAGCTCTGGCTGCCGGTGGCGAAGCCGCGAAGAAAGTTGAGATCCCGGGTGTTGCGACTACCGCATCACCAAGTTCTGAAGTCGGTCGCTTCTTTGAAACCCAGTCATCAAAAGGCGGGCGTTacac                                                                                                                                   <  2:4219674/149‑1 (MQ=255)
                   gCAGATTCCGAAGCAGGACGTGCAGCTCTGGCTGCCGGTGGCGAAGCCGCGAAGAAAGTTGAGATCCCGGGTGTTGCGACTACCGCATCACCAAGTTCTGAAGTCGGTCGCTTCTTTGAAACCCAGTCATCAAAAGGCGGGCGTTacac                                                                                                                                   <  1:4187779/149‑1 (MQ=255)
                   gCAGATTCCGAAGCAGGACGTGCAGCTCTGGCTGCCGGTGGCGAAGCCGCGAAGAAAGTTGAGATCCCGGGTGTTGCGACTACCGCATCACCAAGTTCTGAAGTCGGTCGCTTCTTTGAAACCCAGTCATCAAAAGGCGGGCGTTacac                                                                                                                                   <  2:1604080/149‑1 (MQ=255)
                      gATTCCGAAGCAGGACGTGCAGCTCTGGCTGCCGGTGGCGAAGCCGCGAAGAAAGTTGAGATCCCGGGTGTTGCGACTACCGCATCACCAAGTTCTGAAGTCGGTCGCTTCTTTGAAACCCAGTCATCAAAAGGCGGGCGTTACACCGt                                                                                                                                <  2:886238/149‑1 (MQ=255)
                       aTTCCGAAGCAGGACGTGCAGCTCTGGCTGCCGGTGGCGAAGCCGCGAAGAAAGTTGAGATCCCGGGTGTTGCGACTACCGCATCACCAAGTTCTGAAGTCGGTCGCTTCTTTGAAACCCAGTCATCCAAAGGCGGGCGTTAAACCGTc                                                                                                                               >  2:3920802/1‑149 (MQ=255)
                           cGAAGCAGGACGTGCAGCTCTGGCTGCCGGTGGCGAAGCCGCGAAGAAAGTTGAGATCCCGGGTGTTGCGACTACCGCATCACCAAGTTCTGAAGTCGGTCGCTTCTTTGAAACCCAGTCATCAAAAGGCGGGCGTTACACCGTCACGc                                                                                                                           <  2:4486570/149‑1 (MQ=255)
                               gCAGGACGTGCAGCTCTGGCTGCCGGTGGCGAAGCCGCGAAGAAAGTTGAGATCCCGGGTGTTGCGACTACCGCATCACCAAGTTCTGAAGTCGGTCGCTTCTTTGAAACCCAGTCATCAAAAGGCGGGCGTTACACCGTCACGCTccc                                                                                                                       <  1:1423021/149‑1 (MQ=255)
                               gCAGGACGTGCAGCTCTGGCTGCCGGTGGCGAAGCCGCGAAGAAAGTTGAGATCCCGGGTGTTGCGACTACCGCATCACCAAGTTCTGAAGTCGGTCGCTTCTTTGAAACCCAGTCATCAAAAGGCGGGCGTTACACCGTCACGCTccc                                                                                                                       <  1:1423538/149‑1 (MQ=255)
                                 aGGACGTGCAGCTCTGGCTGCCGGTGGCGAAGCCGCGAAGAAAGTTGAGATCCCGGGTGTTGCGACTACCGCATCACCAAGTTCTGAAGTCGGTCGCTTCTTTGAAACCCAGTCATCCAAAGGCGGGCGTTACACCGTCACGCTCCCgg                                                                                                                     >  2:2543571/1‑149 (MQ=255)
                                 aGGACGTGCAGCTCTGGCTGCCGGTGGCGAAGCCGCGAAGAAAGTTGAGATCCCGGGTGTTGCGACTACCGCATCACCAAGTTCTGAAGTCGGTCGCTTCTTTGAAACCCAGTCATCAAAAGGCGGGCGGTACACCGTCACGCTCCCgg                                                                                                                     >  2:2545103/1‑149 (MQ=255)
                                  ggACGTGCAGCTCTGGCTGCCGGTGGCGAAGCCGCGAAGAAAGTTGAGATCCCGGGTGTTGCGACTACCGCATCACCAAGTTCTGAAGTCGGTCGCTTCTTTTTAACCCAGTCATCAAAAGGCGGGCGTTACACCGTCACGCTCCCGGa                                                                                                                    >  1:680860/1‑149 (MQ=255)
                                  ggACGTGCAGCTCTGGCTGCCGGTGGCGAAGCCGCGAAGAAAGTTGAGATCCCGGGTGTTGCGACTACCGCATCACCAAGTTCTGAAGTCGGTCGCTTCTTTGAAACCCAGTCATCAAAAGGCGGGCGTTACACCGTCACGCTCCCGGa                                                                                                                    >  1:3745172/1‑149 (MQ=255)
                                   gACGTGCAGCTCTGGCTGCCGGTGGCGAAGCCGCGAAGAAAGTTGAGATCCCGGGTGTTGCGACTACCGCATCACCAAGTTCTGAAGTCGGTCGCTTCTTTTAAACCCAGTCAAAAAAAGGCGGGCGTTACACCCTCACGCTCCCGGAt                                                                                                                   >  1:4882799/1‑149 (MQ=255)
                                   gACGTGCAGCTCTGGCTGCCGGTGGCGAAGCCGCGAAGAAAGTTGAGATCCCGGGTGTTGCGACTACCGCATCACCAAGTTCTGAAGTCGGTCGCTTCTTTGAAACCCAGTCATCAAAAGGCGGGCGTTACACCGTCACGCTCCCGGAt                                                                                                                   <  1:779725/149‑1 (MQ=255)
                                        gCAGCTCTGGCTGCCGGTGGCGAAGCCGCGAAGAAAGTTGAGATCCCGGGTGTTGCGACTACCGCATCACCAAGTTCTGAAGTCGGTCGCTTCTTTGAAACCCAGTCATCAAAAGGCGGGCGTTACACCGTCACGCTCCCGGATGGCAc                                                                                                              <  2:1391349/149‑1 (MQ=255)
                                          aGCTCTGGCTGCCGGTGGCGAAGCCGCGAAGAAAGTTGAGATCCCGGGTGTTGCGACTACCGCATCACCAAGTTCTGAAGTCGGTCGCTTCTTTGAAACCCCGTCATCAAAAGGCGGGCGTTACACCCTCACCCTCCCCGATGGCAcaa                                                                                                            >  1:3106415/1‑147 (MQ=255)
                                           gCTCTGGCTGCCGGTGGCGAAGCCGCGAAGAAAGTTGAGATCCCGGGTGTTGCGACTACCGCATCACCAAGTTCTGAAGTCGGTCGCTTCTTTGAAACCCAGTCAACAAAAGGCGGGCGTTACACCGTCACGCTCCCGGATGGCACTaa                                                                                                           >  2:1460843/1‑149 (MQ=255)
                                             tctGGCTGCCGGTGGCGAAGCCGCGAAGAAAGTTGAGATCCCGGGTGTTGCGACTACCGCATCACCAAGTTCTGAAGTCGGTCGCTTCTTTGAAACCCAGTCATCAAAAGGCGGGCGTTACACCGTCACGCTCCCGGATGGCACTAAAg                                                                                                         <  2:186183/149‑1 (MQ=255)
                                             tctGGCTGCCGGTGGCGAAGCCGCGAAGAAAGTTGAGATCCCGGGTGTTGCGACTACCGCATCACCAAGTTCTGAAGTCGGTCGCTTCTTTGAAACCCAGTCATCAAAAGGCGGGCGTTACACCGTCACGCTCCCGGATGGCACTAAAg                                                                                                         <  1:2759490/149‑1 (MQ=255)
                                              ctGGCTGCCGGTGGCGAAGCCGCGAAGAAAGTTGAGATCCCGGGTGTTGCGACTACCGCATCACCAAGTTCTGAAGTCGGTCGCTTCTTTGAAACCCAGTCATCAAAAGGCGGGCGTTACACCGTCACGCTCCCGGATGGCACTAAAGt                                                                                                        <  1:2374520/149‑1 (MQ=255)
                                              ctGGCTGCCGGTGGCGAAGCCGCGAAGAAAGTTGAGATCCCGGGTGTTGCGACTACCGCATCACCAAGTTCTGAAGTCGGTCGCTTCTTTGAAACCCAGTCATCAAAAGGCGGGCGTTACACCGTCACGCTCCCGGATGGCACTAAAGt                                                                                                        <  1:4358619/149‑1 (MQ=255)
                                              ctGGCTGCCGGTGGCGAAGCCGCGAAGAAAGTTGAGATCCCGGGTGTTGCGACTACCGCATCACCAAGTTCTGAAGTCGGTCGCTTCTTTGAAACCCAGTCATCAAAAGGCGGGCGTTACACCGTCACGCTCCCGGATGGCACTAAAGt                                                                                                        <  1:4144976/149‑1 (MQ=255)
                                              ctGGCTGCCGGTGGCGAAGCCGCGAAGAAAGTTGAGATCCCGGGTGTTGCGACTACCGCATCACCAAGTTCTGAAGTCGGTCGCTTCTTTGAAACCCAGTCATCAAAAGGCGGGCGTTACACCGTCACGCTCCCGGATGGCACTAAAGt                                                                                                        <  1:3798278/149‑1 (MQ=255)
                                                    gCCGGTGGCGAAGCCGCGAAGAAAGTTGAGATCCCGGGTGTTGCGACTACCGCATCACCAAGTTCTGAAGTCGGTCGCTTCTTTGAAACCCAGTCATCAAAAGGCGGGCGTTACACCGTCACGCTCCCGGATGGCACTAAAGTCgaaga                                                                                                  <  2:1251540/149‑1 (MQ=255)
                                                      cGGTGGCGAAGCCGCGAAGAAAGTTGAGATCCCGGGTGTTGCGACTACCGCATCACCAAGTTCTGAAGTCGGTCGCTTCTTTGAAACCCAGTCATCAAAAGGCGGGCGTTACACCGTCACGCTCCCGGATGGCACTAAAGTCGAAGAAc                                                                                                <  2:603022/149‑1 (MQ=255)
                                                         tGGCGAAGCCGCGAAGAAAGTTGAGATCCCGGGTGTTGCGACTACCGCATCACCAAGTTCTGAAGTCGGTCGCTTCTTTGAAACCCAGTCATCAAAAGGCGGGCGTTACACCGTCACGCTCCCGGATGGCACTAAAGTCGAAGAACTGa                                                                                             <  2:3871359/149‑1 (MQ=255)
                                                          ggCGAAGCCGCGAAGAAAGTTGAGATCCCGGGTGTTGCGACTACCGCATCACCAAGTTCTGAAGTCGGTCGCTTCTTTGAAACCCAGTCATCAAAAGGCGGGCGTTACACCGTCACGCTCCCGGATGGCACTAAAGTCGAAGAACTGaa                                                                                            <  2:1284532/149‑1 (MQ=255)
                                                          ggCGAAGCCGCGAAGAAAGTTGAGATCCCGGGTGTTGCGACTACCGCATCACCAAGTTCTGAAGTCGGTCGCTTCTTTGAAACCCAGTCATCAAAAGGCGGGCGTTAAACCTTCACGATCCCGGATGGCACTAAAGTCgaagaaattaa                                                                                            >  2:324102/1‑144 (MQ=255)
                                                           gCGAAGCCGCGAAGAAAGTTGAGATCCCGGGTGTTGCGACTACCGCATCACCAAGTTCTGAAGTCGGTCGCTTCTTTGAAACCCAGTCATCAAAAGGCGGGCGTTACACCGTCACGCTCCCGGATGGCACTAAAGTCGAAGAACTGAAc                                                                                           <  1:1623820/149‑1 (MQ=255)
                                                             gAAGCCGCGAAGAAAGTTGAGATCCCGGGTGTTGCGACTACCGCATCACCAAGTTCTGAAGTCGGTCGCTTCTTTGAAACCCAGTCATCAAAAGGCGGGCGTTACACCGTCACGCTCCCGGATGGCACTAAAGTCGAAGAAATGAACaa                                                                                         >  1:4667886/1‑149 (MQ=255)
                                                              aaGCCGCGAAGAAAGTTGAGATCCCGGGTGTTGCGACTACCGCATCACCAAGTTCTGAAGTCGGTCGCTTCTTTGAAACCCAGTCATCAAAAGGCGGGCGTTACACCGTCACGCTCCCGGATGGCACTAAAGTCGAAGAACTGAACaaa                                                                                        <  1:2555623/149‑1 (MQ=255)
                                                               aGCCGCGAAGAAAGTTGAGATCCCGGGTGTTGCGACTACCGCATCACCAAGTTCTGAAGTCGGTCGCTTCTTTGAAACCCAGTCATCAAAAGGCGGGCGTTACACCGTCACGCTCCCGGATGGCACTAAAGTCGAAGAACTGAACAAAg                                                                                       <  1:2661495/149‑1 (MQ=255)
                                                                gCCGCGAAGAAAGTTGAGATCCCGGGTGTTGCGACTACCGCATCACCAAGTTCTGAAGTCGGTCGCTTCTTTGAAACCCAGTCATCAAAAGGCGGGCGTTACACCGTCACGCTCCCGGATGGCACTAAAGTCGAAGAACTGAACAAAGc                                                                                      <  2:4161131/149‑1 (MQ=255)
                                                                    cgAAGAAAGTTGAGATCCGGGGTGTTGCGACTACCGCATCACAAATTTCTGACGTGGGTCGCTTCTTTTAAACCCAGTAATCAAATGGCGGGGGTTACACCGTCACGCTCCCGGATGGCAATAAAGTCGAAGAAATTAACAAAGCGAcc                                                                                  <  2:245292/149‑1 (MQ=255)
                                                                     gaagaaAGTTGAGATCCCGGGTGTTGCGACTACCGCATCACCAAGTTCTGAAGTCGGTCGCTTCTTTGAAACCCAGTCATCAAAAGGCGGGCGTTACACCGTCACGCTCCCGGATGGCACTAAAGTCGAAGAACTGAACAAAGCGACCg                                                                                 >  2:4757969/1‑149 (MQ=255)
                                                                     gaagaaAGTTGAGATCCCGGGTGTTGCGACTACCGCATCACCAAGTTCTGAAGTCGGTCGCTTCTTTGAAACCCAGTCATCAAAAGGCGGGCGTTACACCGTCACGCTCCCGGATGGCACTAAAGTCGAAGAACTGAACAAAGCGACCg                                                                                 >  2:1158235/1‑149 (MQ=255)
                                                                       agaaAGTTGAGATCCCGGGTGTTGCGACTACCGCATCACCAAGTTCTGAAGTCGGTCGCTTCTTTGAAAACCAGTCATCAAAATGCGGGCGTTACACCGTCACGCTCCCGGATGGCACTAAAGTCGAATAACTGAACAAAGCGACCCCa                                                                               >  1:1893343/1‑149 (MQ=255)
                                                                          attttGAGTTCCCGGGTGTTGCGACTACCGAATCACCAAGTTCTTATGTCGGTCGCTTATTTTAAACCCAGTCATCAAAAGGGGGGCGTTAAACCTTCACGCTCCGGGATGGAAATAATGTGGAAGAACTGAAAAAAGCGACCGCAGCg                                                                            <  2:1992537/146‑1 (MQ=255)
                                                                           aGTTGAGAGCGGGGGTGTTGCGACACCCCCAACACCATGTTGTGATGTCGGTCGCTTTTTTGAAACCCAGTCAAAAAAAGGCGGGGGTTACACCGTCAGGCGCCCGGTTGGCAATAATGTCGAAGAACTCAACAAAGCGACCGCAGCga                                                                           <  2:3587393/149‑1 (MQ=255)
                                                                            gTTGAGATCCCGGGTGTTGCGACTACCGCATCACCAAGTTCTGAAGTCGGTCGCTTCTTTGAAACCCAGTCATCAAAAGGCGGGCGTTACACCGTCACGCTCCCGGATGGCACTAAAGTCGAAGAACTGAACAAAGCGACCGCAGCgat                                                                          >  2:3223205/1‑149 (MQ=255)
                                                                             ttGAGATCCCGGGTGTTGCGACTACCGCATCACCAAGTTCTGAAGTCGGTCGCTTCTTTTAAAACCACTAATCAAAATGCGGGGGTTAAACCCTCACCCACCCGGATGGCACAAAAGTAGAAGAAAAGAACAAAACGACCGCACCgctg                                                                         >  1:1773548/1‑149 (MQ=255)
                                                                                  aTCCCGGGTGTTGCGACTACCGCATCACCAAGTTCTGAAGTCGGTCGCTTCTTTGAAACCCAGTGATCAAAAGGCGGGCGTTACACCCTCACGCTCCCGGATGGCACTAAAGTCGAAGAACTGAACAAAGCGCCCCCACCGAAGATGGt                                                                    >  1:3559428/1‑149 (MQ=255)
                                                                                     ccGGGTGTGGCTACTACCGCAAAACATAGTTCTGAAGTCGGTCTATTCTTTGAAACCCAGTCATCAAATGGGGGTCTTTACACCGTCACGCTCCCGGTTGCAACAAATGTCGAATAACTTAAAAAAGCGACCGCAGCGATGATGGTccc                                                                 <  2:213153/149‑1 (MQ=255)
                                                                                         gtgtTGCGACTACCGCATCACCATGTTCTGAAGTCGGTCGCTTCTTTGAAACCCAGTCATCAAAAGGCGGGCGTTACACCGTCACGCTCCCGGATGGCACTAAAGTCGAAGAACTGAACAAAGCGACCGCAGCGATGATGGTCCCGTTc                                                             <  2:2885487/149‑1 (MQ=255)
                                                                                           gtTGCGACTACCGCATCACCAAGTTCTGAAGTCGGTCGCTTCTTTGAAAAACAGTCATCAAAAGGGGGGCGTTACACCGTGACGCTCCCGGGTGGCCCTAAAGTGGAAGTACTGGTCAAAGCGTCCGCAGCGCTGATGGTCCCgggcgt                                                           >  2:1114801/1‑144 (MQ=255)
                                                                                               ctaCTACCGCATCAAAATGTACTTAAGTCGGTCTTTACTTTTAAACCCAGAAAAAAAATGGCGGGCGTAAAACCGTAACGCTCCGGTTTGGCAATAAATTCTAACAACTTAAAAAAGCGACCGCAGCGATGATGGTCCCGTTCGACAGc                                                       <  1:872354/147‑1 (MQ=255)
                                                                                                 aCTACCGCTTCACCAAGTTCTGAAGTCGGTCGCTTCTTTGAAACCCAGTCATCAAAAGGCGGGCGTTACACCGTCACGCTCCCGGATGGCACTAATGTCGAATAACTGAACAAAGCGACCGCAGCGATGATGGTCCCGTTCGACAGCAt                                                     <  2:3343753/149‑1 (MQ=255)
                                                                                                  cTACCGCATCACCAAGTTCTGAAGTCGGTCGCTTCTTTGAAAACCAGTCATCAAAAGGCGGGCGTTACACCGTCACGCTCCCGGATGGCACTAAAGTCGAATAAATGAACAAAGCGACCGCAGCGATGATGGTCCCGTTCGACAGCATc                                                    >  1:2431687/1‑149 (MQ=255)
                                                                                                   tACCGCATCACCAAGTTCTGAAGTCGGTCGCTTCTTTGAAACCCAGTCAACAAAAGGCGGGCGTTACACCGTCACCCTCCCGGATGGCACAAAAGTCGAAGAACTGAACAAAGCGACCGCAGCGATGATTGTCCCGGTCGCCCCCATCa                                                   >  1:4999874/1‑149 (MQ=255)
                                                                                                    aCCGCATCACCAAGTTCTGAAGTCGGTCGCTTCTTTGAAACCCAGTCATAAAAAGGCGGGCGTTACACCGTCACGCTCCCGGATGGCACTAAAGTCGAAGAACTGAACAAAGCGACCCCAGCGATGATGGTCCCGTTCGACAGCATCaa                                                  >  1:2088841/1‑149 (MQ=255)
                                                                                                     ccGCATCCCCAAGTTCTGATGTCGGTCGCTTCTTTGAAACCCAGTCATCAAAAGGCGGGCGTTACACCTTCACGCTCCCGGATGGCACTAACGTCGAATAACTTAACAAAGCGACCGCAGCGATGATGGTCCCGTTCGACAGCATCaaa                                                 <  2:2378776/149‑1 (MQ=255)
                                                                                                       gCATCACAATGTTCTGAAGTCGGTCGCTTCTTTTAAACCCAGTCAACAAATGGCGGGCGTAACACCGTCACGCTCCCGGATGGCACTAAAGTCGAAGAACTGAAAAAAGCGACCGCAGCGATGATGGTCCCGTTCGACAGCATCAAAtt                                               <  2:1609179/149‑1 (MQ=255)
                                                                                                          tCACCAAGTTGTTATGTCGGTCGCTTCTTTTAAACCCAGTAATCAAATGGCGGGCGTTACACCGTCACGCTCCCGTTTGGCACTAAAGTCGAACAACTGAACAACGCGACCGCAGCGATGATGGTCCCGTTCGACAGCATCAAATtctc                                            <  2:191570/149‑1 (MQ=255)
                                                                                                          tCACCAAGTTCTGAAGTCGGTCGCTTATTTGAAACCCAGTCATCAAAATGCGGGCGTTACACCGGCACGCACCCGGATGGCAATAAATTCGAAGAAATGAACAAAGCGACCGCAGCGAATATGGTCCCGTTCGACAGCATCCAAAtctc                                            >  2:3477213/1‑149 (MQ=255)
                                                                                                          tCACCAAGTTCTGAAGTCGGTCGCTTATTTGAAACCCAGTCATCAAAAGGCGGGCGTTACACCGTCACGCTCCCGGATGGCACTAAAGTCGAATAACTGAACAAAGCGACCGCAGCGATGATGGTCCCGTTCGACAGCATCAAATtctc                                            <  2:2782834/149‑1 (MQ=255)
                                                                                                            agtttgTTGTGATGTCGGTCGGTTTTTTTAAAGCCAGTCTTAATATGGGGGGGGTTACACCGTCAGGGTCCGGTTTGGAACTTATTTCTAAGAACTGACCACCGCGACCGCAGCGATGATGGTCCCGTTCGACAGCATCAAATTCTCTg                                          <  2:5050289/144‑1 (MQ=255)
                                                                                                             ccattttCTTACGTCGTTCGCTTATTTTAAACCAATTCATCAAAAGGCGGGTGTTACACCGTCACGCACCCGGATGGCAAAAACGACTACTAAATGAACAACGCGACCGCAGCGATGATGGTCCCGTTCGACAGCATCAAATTCTCTgg                                         <  2:1790781/144‑1 (MQ=255)
                                                                                                              cAAGTTCTTAAGTCGGTCGCTTCTTTGAAACCCAGTCATCAAAAGGCGGGCGTTACACCGTCACGCTCCCGTATGGCACTAAAGTCGAAGAACTGAACAAAGCGACCGCAGCGATGATGGTCCCGTTCGACAGCATCAAATTCTCTGGc                                        <  2:258818/149‑1 (MQ=255)
                                                                                                              cAAGTTATTAAGTCGGTCTCTTTTTTTAAACCCCGTCATCAATTGGCGGGCGTTACACCGTCACGCTCCCGGATGGCAATAACTGCGAACACCTCAACACCGCGACCGCAGCGATGATGGTCCCGTTCGACAGCATCAAATTCTCTGGc                                        <  2:9278/149‑1 (MQ=255)
                                                                                                                 gTTCTTATGTCGGGCGCTTATTTGAAACCCAGTCATCAAAAGGCGGGCGTTACCCCGTCACGCTCCCGGTTGGCACTAAAGTCTAAGAACTGAACAACGCGACCGCAGCGATGATGGTCCCGTTCGACAGCATCAAATTCTCTGGCAAc                                     <  2:3172836/149‑1 (MQ=255)
                                                                                                                 gTTCTGAAGTCGGTCGCTTCTTTGAAACCCAGTCATCAAATGGCGGGCTTTACACCTTCACGCTCCGGTATGGCACAAAAGTCTAACAACTTAACAACGCGACCGCAGCGATGATGGTCCCGTTCGACAGCATCAAATTCTCTGGCAAc                                     <  2:4950622/149‑1 (MQ=255)
                                                                                                                  ttCTGACGTGGGTCGTTTATTTTAAACCCAGTAATCAAACGGCGGGGTTTCCACCGACACGCCCCGGTTTGGCACTAAAGTCGAATAACTTAACAAAGCGACCGCAGCGATGATGGTCCCGTTCGACAGCATCAAATTCTCTGGCAACt                                    <  1:3768960/149‑1 (MQ=255)
                                                                                                                  ttCTGAAGTCGGTCGCTTCTTTGAACCCCCGTCATCAAAAGGCGGGCGTTACACCGTCACGCTCCCGGATGGCACTAAAGTCGAAGAACTGAACAAAGCGACCGCAGCGATGATGGTCCCGTTCGACAGCATCAAATTCTCTGGCAACt                                    <  2:4710964/149‑1 (MQ=255)
                                                                                                                   tCTGAAGTCGGTCGCTTCTTTGAAACCCAGTCATAAAAAGGCGGGCGTTACCCCGTCACGCGCCCGGATGGCACTAAAGTCGAAGAACTGAACAAAGCGACCGCAGCGATGATGGTCCCGTTCGACAGCATCAAATTCTCTGGCAACTa                                   <  2:2821017/149‑1 (MQ=255)
                                                                                                                       atgtcggtcgCTTCTTTGAAACCCAGTCATAAAAAGGCGGGCGTTACACCGTCACGCTCCCGGATGGCACTAAAGTCGAAGAACTGAACAAAGCGACCGCAGCGATGATGGTCCCGTTCGACAGCATCAAATTCTCTGGCAACTACGGc                               <  2:3766875/147‑1 (MQ=255)
                                                                                                                       aaGTCGGTCGCGTCTTTGAAACCCAGTCATCAAAAGGCGGGCGTTAAACCGGCACCCTCCCCGATGGCCCTAAATTCGAAGAACTGAAAAAATCGACCGCAGCGATTATGGTCCCGGTCGACAGCATCAAATTCTCTGGCAAAAACGGc                               >  2:2178968/1‑149 (MQ=255)
                                                                                                                        aGTCGGTCGCTTCTTTGAAACCCAGTCATAAAAAGGCGGGCGTTACACCGTCACGCTCCCGGATGGCACAAAATTCGAAGAACTGAACAAAGCCACCCCAGCGATGATGGTCCCGTTCGACAGCATCAAATTCTCTGGCAACAACGGCa                              >  2:2332891/1‑149 (MQ=255)
                                                                                                                           gggtcgCTTATTTGAAACCATGTCATCAAACGGCGGGGTTTACACCTTCACGCTCCGGGATGGCACTAAAGTCGACTAACTTAACAAAGCGACCGCAGCGATGATGGTCCCGTTCGACAGCATCAAATTCTCTGGCAACTACGGCAACa                           <  1:3017518/148‑1 (MQ=255)
                                                                                                                             gtcgCTTCTTTGAAACCCAGTCATCAAAAGGCGGGCGTTACACCGTCACGCTCCCGGATGGCACTAAAGTCGAAGAACTGAACAAAGCGACCGCAGCGATGATGGTCCCGTTCGACAGCATCAAATTCTCTGGCAACTACGGCAACATg                         >  2:352877/1‑149 (MQ=255)
                                                                                                                             gtcgCTTCTTTGAAACCCAGTCATCAAAAGGCGGGCGTTACACCGTCACGCTCCCGGATGGCACTAAAGTCGAAGAACTGAACAAAGCGACCGCAGCGATGATGGTCCCGGTCGACAGCATCAAAATCTCTGGCAACTACGGCAACATg                         >  2:2938653/1‑149 (MQ=255)
                                                                                                                             gtcgCTTATTTTAAACCCATTCATAAATTGGGGGGGTTTACACCGTCACGCGCCCGTATGGCAATAAAGTCGAAGAACTTAACAACGCGACCGCAGCGATGATGGTCCCGTTCGACAGCATCAAATTCTCTGGCAACTACGGCAACATg                         <  2:4842921/149‑1 (MQ=255)
                                                                                                                              tggattcttTTAACCCCTGTCATAAAATGGCGGGCTTTACCCCGGCACGCTCCGGGATGGAAATAATGTCGAACAACTTAAAAACGCGACCGCAGCGATGATGGTCCCGTTCGACAGCATCAAATTCTCTGGCAACTACGGCAACATGa                        <  1:4085383/145‑1 (MQ=255)
                                                                                                                               cgCTTCTTTGAAACCCAGTCATCAAAAGGCGGGCGTTACACCGTCACGCTCCCGGATGGCACTAAAGTCGAAGAACTGAACAAAGCGACCGCAGCGATGATGGTCCCGTTCGACAGCATCAAATTCTCTGGCAACTACGGCAACATGAc                       >  2:861627/1‑149 (MQ=255)
                                                                                                                               cgCTTCTTTGAAACCCAGTCATCAAAAGGCGGGCGTTACACCGTCACGCTCCCGGATGGCACTAAAGTCGAAGAACTGAACAAAGCGACCGCAGCGATGATGGTCCCGTTCGACAGCATCAAATTCTCTGGCAACTACGGCAACATGAc                       >  1:1165583/1‑149 (MQ=255)
                                                                                                                                gCTTCTTTGAAACCCAGTCATCAAAAGGCGGGCGTTACACCGTCACGCTCCCGGATGGCACTAAAGTCGAAGAACTGAACAAAGCGACCGCAGCGATGATGGTCCCGTTCGACAGCATCAAATTCTCTGGCAACTACGGCAACATGAcc                      >  2:4380035/1‑149 (MQ=255)
                                                                                                                                 cttcttTGAAACCCAGTCATCAAAAGGCGGGCGTTACACCGTCACGCTCCCGGATGGCACTAAAGTCGAAGAACTGAACAAAGCGACCGCAGCGATGATGGTCCCGTTCGACAGCATCAAATTCTCTGGCAACTACGGCAACATGACCg                     >  1:1515941/1‑149 (MQ=255)
                                                                                                                                 cttcttTGAAACCCAGTCATCAAAAGGCGGGCGTTACACCGTCACGCTCCCGGATGGCACTAAAGTCGAAGAACTGAACAAAGCGACCGCAGCGATGATGGTCCCGTTCGACAGCATCAAATTCTCTGGCAACTACGGCAACATGACCg                     >  1:440898/1‑149 (MQ=255)
                                                                                                                                   tcttTGAAACCCAGTCATCAAATGGCGGGGGTTACACCGTCACGCTCCGGTATGGCACTAAAGTCGAAGAACTTAACAAAGCGACCGCAGCGATGATGGTCCCGTTCGACAGCATCAAATTCTCTGGCAACTACGGCAACATGACCGaa                   <  1:5156456/149‑1 (MQ=255)
                                                                                                                                   tcttTGAAACCCAGTCATCAAAAGGCGGGCGTTACACCGTCACGCTCCCGGATGGCACTAAAGTCGAAGAACTGAACAAAGCGACCGCAGCGATGATGGTCCCGTTCGACAGCATCAAATTCTCTGGCAACTACGGCAACATGACCGaa                   >  2:723998/1‑149 (MQ=255)
                                                                                                                                      ttcaaccccAGTAAAAAAAGGGGGGGCTTAACACCGTCCCGCGCCGGGTTGCCACTAACGTCCAAGAACTGAACAAAGCGACCGCAGCGATGATGGTCCCGTTCGACAGCATCAAATTCTCTGGCAACTACGGCAACATGACCGAAGtc                <  1:610239/143‑1 (MQ=255)
                                                                                                                                        taaaCCCAGTCATAAAATGGGGGGCGTTACACCGACACGCGCCCGGTTGGACCTAACGTCGAACAACTTAACAAAGCGACCGCAGCGATGATGGTCCCGTTCGACAGCATCAAATTCTCTGGCAACTACGGCAACATGACCGAAGtctc              <  1:741621/148‑1 (MQ=255)
                                                                                                                                        gAACCCCATACATCAACGGGGGGGCTTAACACCGCCCCGCTCCCGGTTGCCACAAACGTCCAATAACTGAACAACGCGACCGCAGCGATGATGGTCCCGTTCGACAGCATCAAATTCTCTGGCAACTACGGCAACATGACCGAAGtctc              <  1:218588/149‑1 (MQ=255)
                                                                                                                                          aaCCCAGTCATCAAAAGGCGGGCGTTACACCGTCACGCTCCCGGATGGCACTAAAGTCGAAGAACTGAACAAAGCGACCGCAGCGATGATGGTCCCGTTCGACAGCATCAAATTCTCTGGCAACTACGGCAACATGACCGAAGTCTCCt            <  2:3383240/149‑1 (MQ=255)
                                                                                                                                          aaCCCAGTCATCAAAAGGCGGGCGTTACACCGACACGCTCCCGTATGGCACTAAAGTCGAAGAACTGAACAAAGCGACCGCAGCGATGATGGTCCCGTTCGACAGCATCAAATTCTCTGGCAACTACGGCAACATGACCGAAGTCTCCt            <  2:1638125/149‑1 (MQ=255)
                                                                                                                                            cccAGTCATCAAATGGCGGGCGTTACACCGTCACGCTCCGGGATGGCACTAACGTCGAAGAACTGAACAAAGCGACCGCAGCGATGATGGTCCCGTTCGACAGCATCAAATTCTCTGGCAACTACGGCAACATGACCGAAGTCTCCTAt          <  2:3743599/149‑1 (MQ=255)
                                                                                                                                            cccAGTCATCAAACGGCGGGCGTTACACCGTCACGCTCCCGGATGGCACTAAAGTCTAAGAACTGAACAAAGCGACCGCAGCGATGATGGTCCCGTTCGACAGCATCAAATTCTCTGGCAACTACGGCAACATGACCGAAGTCTCCTAt          <  2:680899/149‑1 (MQ=255)
                                                                                                                                             cccgTCATAAATTGGGGGGCGTTACACCTTCACGCTCCCGTTTGGCAATAATGTCGAAGAACTTAACAAAGCGACCGCAGCGATGATGGTCCCGTTCGACAGCATCAAATTCTCTGGCAACTACGGCAACATGACCGAAGTCTCCTATc         <  2:3935916/146‑1 (MQ=255)
                                                                                                                                                 tcatcaAAAGGCGGGCGTTACACCGTCACGCTCCCGGATGGCACTAAAGTCGAAGAACTGAACAAAGCGACCGCAGCGATGATGGTCCCGTTCGACAGCATCAAATTCTCTGGCAACTACGGCAACATGACCGAAGTCTCCTATCAGGt     >  1:2881594/1‑149 (MQ=255)
                                                                                                                                                  catcaAAAGGCGGGCGTTACACCGTCACGCTCCCGGATGGCACTAAAGTCGAAGAACTGAACAAAGCGACCGCAGCGATGATGGTCCCGTTCGACAGCATCAAATTCTCTGGCAACTACGGCAACATGACCGAAGTCTCCTATCAGGtt    >  1:340097/1‑149 (MQ=255)
                                                                                                                                                    tcaAAAGGCGGGCGTTACACCGTCACGCTCCCGGATGGCACTAAAGTCGAAGAACTGAACAAAGCGACCGCAGCGATGATGGTCCCGTTCGACAGCATCAAATTCTCTGGCAACTACGGCAACATGACCGAAGTCTCCTATCAGGTTGc  >  2:4950025/1‑149 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
GAGCCCGGATGTGATCCCGGCAGATTCCGAAGCAGGACGTGCAGCTCTGGCTGCCGGTGGCGAAGCCGCGAAGAAAGTTGAGATCCCGGGTGTTGCGACTACCGCATCACCAAGTTCTGAAGTCGGTCGCTTCTTTGAAACCCAGTCATCAAAAGGCGGGCGTTACACCGTCACGCTCCCGGATGGCACTAAAGTCGAAGAACTGAACAAAGCGACCGCAGCGATGATGGTCCCGTTCGACAGCATCAAATTCTCTGGCAACTACGGCAACATGACCGAAGTCTCCTATCAGGTTGC  >  CP009273/1673232‑1673528

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑