Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,673,381 C→A 8.0% S233* (TCA→TAA) ‡ ydgH → DUF1471 family periplasmic protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,673,3810CA 8.0% 642.0 / 4.4 226S233* (TCA→TAA) ‡ydgHDUF1471 family periplasmic protein
Reads supporting (aligned to +/- strand):  ref base C (82/114);  new base A (8/22);  total (90/136)
Fisher's exact test for biased strand distribution p-value = 1.60e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 235 total aligned reads displayed.

AGCCCGGATGTGATCCCGGCAGATTCCGAAGCAGGACGTGCAGCTCTGGCTGCCGGTGGCGAAGCCGCGAAGAAAGTTGAGATCCCGGGTGTTGCGACTACCGCATCACCAAGTTCTGAAGTCGGTCGCTTCTTTGAAACCCAGTCATCAAAAGGCGGGCGTTACACCGTCACGCTCCCGGATGGCACTAAAGTCGAAGAACTGAACAAAGCGACCGCAGCGATGATGGTCCCGTTCGACAGCATCAAATTCTCTGGCAACTACGGCAACATGACCGAAGTCTCCTATCAGGTTGCG  >  CP009273/1673233‑1673529
                                                                                                                                                    |                                                                                                                                                    
    cGGATGTGATCCCGGCAGATTCCGAAGCAGGACGTGCAGCTCTGGCTGCCGGTGGCGAAGCCGCGAAGAAAGTTGAGATCCCGGGTGTTGCGACTACCGCATCACCAAGTTCTGAAGTCGGTCGCTTCTTTGAAACCCAGTCATCaaaa                                                                                                                                                  >  2:2483591/1‑149 (MQ=255)
     ggATGTGATCCCGGCAGATTCCGAAGCAGGACGTGCAGCTCTGGCTGCCGGTGGCGAAGCCGCGAAGAAAGTTGAGATCCCGGGTGTTGCGACTACCGCATCACCAAGTTCTGAAGTCGGTCGCTTCTTTGAAACCCAGTCATCAAAAg                                                                                                                                                 <  2:1433055/149‑1 (MQ=255)
       aTGTGATCCCGGCAGATTCCGAAGCAGGACGTGCAGCTCTGGCTGCCGGTGGCGAAGCCGCGAAGAAAGTTGAGATCCCGGGTGTTGCGACTACCGCATCACCAAGTTCTGAAGTCGGTCGCTTCTTTGAAACCCAGTCATCAAAAggc                                                                                                                                               <  1:1534495/149‑1 (MQ=255)
        tgtgATCCCGGCAGATTCCGAAGCAGGACGTGCAGCTCTGGCTGCCGGTGGCGAAGCCGCGAAGAAAGTTGAGATCCCGGGTGTTGCGACTACCGCATCACCAAGTTCTGAAGTCGGTCGCTTCTTTGAAACCCAGTCATCAAAAggcg                                                                                                                                              <  1:485875/149‑1 (MQ=255)
         gtgATCCCGGCAGATTCCGAAGCAGGACGTGCAGCTCTGGCTGCCGGTGGCGAAGCCGCGAAGAAAGTTGAGATCCCGGGTGTTGCGACTACCGCATCACCAAGTTCTGAAGTCGGTCGCTTCTTTGAAACCCAGTCATCAAAAggcgg                                                                                                                                             <  2:522087/149‑1 (MQ=255)
           gATCCCGGCAGATTCCGAAGCAGGACGTGCAGCTCTGGCTGCCGGTGGCGAAGCCGCGAAGACAGTTGAGATCCCGGGTGTTGCGACTACCGCATCACCAAGTTCTGAAGTCGGTCGCTTCTTTGAAACCCAGTCATCAAAAggcgggc                                                                                                                                           <  2:4415407/149‑1 (MQ=255)
            aTCCCGGCAGATTCCGAAGCAGGACGTGCAGCTCTGGCTGCCGGTGGCGAAGCCGCGAAGAAAGTTGAGATCCCGGGTGTTGCGACTACCGCATCACCAAGTTCTGAAGTCGGTCGCTTCTTTGAAACCCAGTCATCAAAAggcgggcg                                                                                                                                          <  2:3599365/149‑1 (MQ=255)
             tCCCGGCAGATTCCGAAGCAGGACGTGCAGCTCTGGCTGCCGGTGGCGAAGCCGCGAAGAAAGTTGAGATCCCGGGTGTTGCGACTACCGCATCACCAAGTTCTGAAGTCGGTCGCTTCTTTGAAACCCAGTCATCAAAAGGCGGGCGt                                                                                                                                         >  2:2518666/1‑149 (MQ=255)
             tCCCGGCAGATTCCGAAGCAGGACGTGCAGCTCTGGCTGCCGGTGGCGAAGCCGCGAAGAAAGTTGAGATCCCGGGTGTTGCGACTACCGCATCACCAAGTTCTGAAGTCGGTCGCTTCTTTGAAACCCAGTCATCAAAAGGCGGGCGt                                                                                                                                         >  2:2518215/1‑149 (MQ=255)
              cccGGCAGATTCCGAAGCAGGACGTGCAGCTCTGGCTGCCGGTGGCGAAGCCGCGAAGAAAGTTGAGATCCCGGGTGTTGCGACTACCGCATCACCAAGTTCTGACGTCGGTCGCTTCTTTGAAACCCAGTCATCAAAAGGCGGGCGtt                                                                                                                                        >  2:1962757/1‑149 (MQ=255)
               ccGGCAGATTCCGAAGCAGGACGTGCAGCTCTGGCTGCCGGTGGCGAAGCCGCGAAGAAAGTTGAGATCCCGGGTGTTGCGACTACCGCATCACCAAGTTCTGAAGTCGGTCGCTTCTTTGAAACCCAGTCATCAAAAGGCGGGCGTTa                                                                                                                                       <  1:935703/149‑1 (MQ=255)
                  gCAGATTCCGAAGCAGGACGTGCAGCTCTGGCTGCCGGTGGCGAAGCCGCGAAGAAAGTTGAGATCCCGGGTGTTGCGACTACCGCATCACCAAGTTCTGAAGTCGGTCGCTTCTTTGAAACCCAGTCATCAAAAGGCGGGCGTTacac                                                                                                                                    >  2:3077597/1‑149 (MQ=255)
                  gCAGATTCCGAAGCAGGACGTGCAGCTCTGGCTGCCGGTGGCGAAGCCGCGAAGAAAGTTGAGATCCCGGGTGTTGCGACTACCGCATCACCAAGTTCTGAAGTCGGTCGCTTCTTTGAAACCCAGTCATCAAAAGGCGGGCGTTacac                                                                                                                                    <  1:4187779/149‑1 (MQ=255)
                     gATTCCGAAGCAGGACGTGCAGCTCTGGCTGCCGGTGGCGAAGCCGCGAAGAAAGTTGAGATCCCGGGTGTTGCGACTACCGCATCACCAAGTTCTGAAGTCGGTCGCTTCTTTGAAACCCAGTCATCAAAAGGCGGGCGTTACACCGt                                                                                                                                 <  2:886238/149‑1 (MQ=255)
                          cGAAGCAGGACGTGCAGCTCTGGCTGCCGGTGGCGAAGCCGCGAAGAAAGTTGAGATCCCGGGTGTTGCGACTACCGCATCACCAAGTTCTGAAGTCGGTCGCTTCTTTGAAACCCAGTCATCAAAAGGCGGGCGTTACACCGTCACGc                                                                                                                            <  2:4486570/149‑1 (MQ=255)
                             aGCAGGACGTGCAGCTCTGGCTGCCGGTGGCGAAGCCGCGAAGAAAGTTGAGATCCCGGGTGTTGCGACTACCGCATCACCAAGTTCTGAAGTCGGTCGCTTCTTTGAAACCCAGTCATCAAAAGGCGGGCGTTACACCGTCACGCTcc                                                                                                                         <  2:335950/149‑1 (MQ=255)
                              gCAGGACGTGCAGCTCTGGCTGCCGGTGGCGAAGCCGCGAAGAAAGTTGAGATCCCGGGTGTTGCGACTACCGCATCACCAAGTTCTGAAGTCGGTCGCTTCTTTGAAACCCAGTCATCAAAAGGCGGGCGTTACACCGTCACGCTccc                                                                                                                        <  1:1423538/149‑1 (MQ=255)
                              gCAGGACGTGCAGCTCTGGCTGCCGGTGGCGAAGCCGCGAAGAAAGTTGAGATCCCGGGTGTTGCGACTACCGCATCACCAAGTTCTGAAGTCGGTCGCTTCTTTGAAACCCAGTCATCAAAAGGCGGGCGTTACACCGTCACGCTccc                                                                                                                        <  1:1423021/149‑1 (MQ=255)
                                aGGACGTGCAGCTCTGGCTGCCGGTGGCGAAGCCGCGAAGAAAGTTGAGATCCCGGGTGTTGCGACTACCGCATCACCAAGTTCTGAAGTCGGTCGCTTCTTTGAAACCCAGTCATCAAAAGGCGGGCGTTACACCGTCACGCTCCCgg                                                                                                                      >  2:2844489/1‑149 (MQ=255)
                                aGGACGTGCAGCTCTGGCTGCCGGTGGCGAAGCCGCGAAGAAAGTTGAGATCCCGGGTGTTGCGACTACCGCATCACCAAGTTCTGAAGTCGGTCGCTTCTTTGAAACCCAGTCATCAAAAGGCGGGCGTTACACCGTCACGCTCCCgg                                                                                                                      >  2:3594333/1‑149 (MQ=255)
                                aGGACGTGCAGCTCTGGCTGCCGGTGGCGAAGCCGCGAAGAAAGTTGAGATCCCGGGTGTTGCGACTACCGCATCACCAAGTTCTGAAGTCGGTCGCTTCTTTGAAACCCAGTCATCAAAAGGCGGGCGGTACACCGTCACGCTCCCgg                                                                                                                      >  2:2545103/1‑149 (MQ=255)
                                 ggACGTGCAGCTCTGGCTGCCGGTGGCGAAGCCGCGAAGAAAGTTGAGATCCCGGGTGTTGCGACTACCGCATCACCAAGTTCTGAAGTCGGTCGCTTCTTTTTAACCCAGTCATCAAAAGGCGGGCGTTACACCGTCACGCTCCCGGa                                                                                                                     >  1:680860/1‑149 (MQ=255)
                                 ggACGTGCAGCTCTGGCTGCCGGTGGCGAAGCCGCGAAGAAAGTTGAGATCCCGGGTGTTGCGACTACCGCATCACCAAGTTCTGAAGTCGGTCGCTTCTTTGAAACCCAGTCATCAAAAGGCGGGCGTTACACCGTCACGCTCCCGGa                                                                                                                     >  1:3745172/1‑149 (MQ=255)
                                  gACGTGCAGCTCTGGCTGCCGGTGGCGAAGCCGCGAAGAAAGTTGAGATCCCGGGTGTTGCGACTACCGCATCACCAAGTTCTGAAGTCGGTCGCTTCTTTTAAACCCAGTCAAAAAAAGGCGGGCGTTACACCCTCACGCTCCCGGAt                                                                                                                    >  1:4882799/1‑149 (MQ=255)
                                  gACGTGCAGCTCTGGCTGCCGGTGGCGAAGCCGCGAAGAAAGTTGAGATCCCGGGTGTTGCGACTACCGCATCACCAAGTTCTGAAGTCGGTCGCTTCTTTGAAACCCAGTCATCAAAAGGCGGGCGTTACACCGTCACGCTCCCGGAt                                                                                                                    <  1:779725/149‑1 (MQ=255)
                                         aGCTCTGGCTGCCGGTGGCGAAGCCGCGAAGAAAGTTGAGATCCCGGGTGTTGCGACTACCGCATCACCAAGTTCTGAAGTCGGTCGCTTCTTTGAAACCCCGTCATCAAAAGGCGGGCGTTACACCCTCACCCTCCCCGATGGCAcaa                                                                                                             >  1:3106415/1‑147 (MQ=255)
                                            tctGGCTGCCGGTGGCGAAGCCGCGAAGAAAGTTGAGATCCCGGGTGTTGCGACTACCGCATCACCAAGTTCTGAAGTCGGTCGCTTCTTTGAAACCCAGTCATCAAAAGGCGGGCGTTACACCGTCACGCTCCCGGATGGCACTAAAg                                                                                                          <  2:186183/149‑1 (MQ=255)
                                            tctGGCTGCCGGTGGCGAAGCCGCGAAGAAAGTTGAGATCCCGGGTGTTGCGACTACCGCATCACCAAGTTCTGAAGTCGGTCGCTTCTTTGAAACCCAGTCATCAAAAGGCGGGCGTTACACCGTCACGCTCCCGGATGGCACTAAAg                                                                                                          <  1:2759490/149‑1 (MQ=255)
                                             ctGGCTGCCGGTGGCGAAGCCGCGAAGAAAGTTGAGATCCCGGGTGTTGCGACTACCGCATCACCAAGTTCTGAAGTCGGTCGCTTCTTTGAAACCCAGTCATCAAAAGGCGGGCGTTACACCGTCACGCTCCCGGATGGCACTAAAGt                                                                                                         <  1:3798278/149‑1 (MQ=255)
                                             ctGGCTGCCGGTGGCGAAGCCGCGAAGAAAGTTGAGATCCCGGGTGTTGCGACTACCGCATCACCAAGTTCTGAAGTCGGTCGCTTCTTTGAAACCCAGTCATCAAAAGGCGGGCGTTACACCGTCACGCTCCCGGATGGCACTAAAGt                                                                                                         <  1:2374520/149‑1 (MQ=255)
                                             ctGGCTGCCGGTGGCGAAGCCGCGAAGAAAGTTGAGATCCCGGGTGTTGCGACTACCGCATCACCAAGTTCTGAAGTCGGTCGCTTCTTTGAAACCCAGTCATCAAAAGGCGGGCGTTACACCGTCACGCTCCCGGATGGCACTAAAGt                                                                                                         <  1:4144976/149‑1 (MQ=255)
                                             ctGGCTGCCGGTGGCGAAGCCGCGAAGAAAGTTGAGATCCCGGGTGTTGCGACTACCGCATCACCAAGTTCTGAAGTCGGTCGCTTCTTTGAAACCCAGTCATCAAAAGGCGGGCGTTACACCGTCACGCTCCCGGATGGCACTAAAGt                                                                                                         <  1:4358619/149‑1 (MQ=255)
                                             ctGGCTGCCGGTGGCGAAGCCGCGAAGAAAGTTGAGATCCCGGGTGTTGCGACTACCGCATCACCAAGTTCTGAAGTCGGTCGCTTCTTTGAAACCCAGTCATCAAAAGGCGGGCGTTACACCGTCACGCTCCCGGATGGCACTAAAGt                                                                                                         <  2:131427/149‑1 (MQ=255)
                                                  tGCCGGTGGCGAAGCCGCGAAGAAAGTTGAGATCCCGGGTGTTGCGACTACCGCATCACCAAGTTCTGAAGTCGGTCGCTTCTTTGAAACCCAGTCATCAAAAGGCGGGCGTTACACCGTCACGCTCCCGGATGGCACTAAAGTCgaag                                                                                                    >  2:3503375/1‑149 (MQ=255)
                                                     cGGTGGCGAAGCCGCGAAGAAAGTTGAGATCCCGGGTGTTGCGACTACCGCATCACCAAGTTCTGAAGTCGGTCGCTTCTTTGAAACCCAGTCATCAAAAGGCGGGCGTTACACCGTCACGCTCCCGGATGGCACTAAAGTCGAAGAAc                                                                                                 <  2:603022/149‑1 (MQ=255)
                                                       gTGGCGAAGCCGCGAAGATAGTTGAGATCCCGGGTGTTGCGACTACCGCATCACCAAGTTCTGAAGTCGGTCGCTTCTTTGAAACCCAGTCATCAAAAGGCGGGCGTTACACCGTCACGCTCCCGGATGGCACTAAAGTCGAAGAACTg                                                                                               <  2:3808373/149‑1 (MQ=255)
                                                         ggCGAAGCCGCGAAGAAAGTTGAGATCCCGGGTGTTGCGACTACCGCATCACCAAGTTCTGAAGTCGGTCGCTTCTTTGAAACCCAGTCATCAAAAGGCGGGCGTTACACCGTCACGCTCCCGGATGGCACTAAAGTCGAAGAACTGaa                                                                                             <  2:3708142/149‑1 (MQ=255)
                                                         ggCGAAGCCGCGAAGAAAGTTGAGATCCCGGGTGTTGCGACTACCGCATCACCAAGTTCTGAAGTCGGTCGCTTCTTTGAAACCCAGTCATCAAAAGGCGGGCGTTACACCGTCACGCTCCCGGATGGCACTAAAGTCGAAGAACTGaa                                                                                             >  2:2913941/1‑149 (MQ=255)
                                                          gCGAAGCCGCGAAGAAAGTTGAGATCCCGGGTGTTGCGACTACCGCATCACCAAGTTCTGAAGTCGGTCGCTTCTTTGAAACCCAGTCATCAAAAGGCGGGCGTTACACCGTCACGCTCCCGGATGGCACTAAAGTCGAAGAACTGAAc                                                                                            <  1:1623820/149‑1 (MQ=255)
                                                            gAAGCCGCGAAGAAAGTTGAGATCCCGGGTGTTGCGACTACCGCATCACCAAGTTCTGAAGTCGGTCGCTTCTTTGAAACCCAGTCATCAAAAGGCGGGCGTTACACCGTCACGCTCCCGGATGGCACTAAAGTCGAAGAACTTAACaa                                                                                          >  2:1774741/1‑149 (MQ=255)
                                                            gAAGCCGCGAAGAAAGTTGAGATCCCGGGTGTTGCGACTACCGCATCACCAAGTTCTGAAGTCGGTCGCTTCTTTGAAACCCAGTCATCAAAAGGCGGGCGTTACACCGTCACGCTCCCGGATGGCACTAAAGTCGAAGAAATGAACaa                                                                                          >  1:4667886/1‑149 (MQ=255)
                                                             aaGCCGCGAAGAAAGTTGAGATCCCGGGTGTTGCGACTACCGCATCACCAAGTTCTGAAGTCGGTCGCTTCTTTGAAACCCAGTCATCAAAAGGCGGGCGTTACACCGTCACGCTCCCGGATGGCACTAAAGTCGAAGAACTGAACaaa                                                                                         <  1:2555623/149‑1 (MQ=255)
                                                              aGCCGCGAAGAAAGTTGAGATCCCGGGTGTTGCGACTACCGCATCACCAAGTTCTGAAGTCGGTCGCTTCTTTGAAACCCAGTCATCAAAAGGCGGGCGTTACACCGTCACGCTCCCGGATGGCACTAAAGTCGAAGAACTGAACAAAg                                                                                        <  2:3925566/149‑1 (MQ=255)
                                                              aGCCGCGAAGAAAGTTGAGATCCCGGGTGTTGCGACTACCGCATCACCAAGTTCTGAAGTCGGTCGCTTCTTTGAAACCCAGTCATCAAAAGGCGGGCGTTACACCGTCACGCTCCCGGATGGCACTAAAGTCGAAGAACTGAACAAAg                                                                                        <  2:3409009/149‑1 (MQ=255)
                                                              aGCCGCGAAGAAAGTTGAGATCCCGGGTGTTGCGACTACCGCATCACCAAGTTCTGAAGTCGGTCGCTTCTTTGAAACCCAGTCATCAAAAGGCGGGCGTTACACCGTCACGCTCCCGGATGGCACTAAAGTCGAAGAACTGAACAAAg                                                                                        <  1:2661495/149‑1 (MQ=255)
                                                               gCCGCGAAGAAAGTTGAGATCCCGGGTGTTGCGACTACCGCATCACCAAGTTCTGAAGTCGGTCGCTTCTTTGAAACCCAGTCATCAAAAGGCGGGCGTTACACCGTCACGCTCCCGGATGGCACTAAAGTCGAAGAACTGAACAAAGc                                                                                       <  2:1548071/149‑1 (MQ=255)
                                                                   cgAAGAAAGTTGAGATCCGGGGTGTTGCGACTACCGCATCACAAATTTCTGACGTGGGTCGCTTCTTTTAAACCCAGTAATCAAATGGCGGGGGTTACACCGTCACGCTCCCGGATGGCAATAAAGTCGAAGAAATTAACAAAGCGAcc                                                                                   <  2:245292/149‑1 (MQ=255)
                                                                    gaagaaAGTTGAGATCCCGGGTGTTGCGACTACCGCATCACCAAGTTCTGAAGTCGGTCGCTTCTTTGAAACCCAGTCATCAAAAGGCGGGCGTTACACCGTCACGCTCCCGGATGGCACTAAAGTCGAAGAACTGAACAAAGCGACCg                                                                                  >  2:1158363/1‑149 (MQ=255)
                                                                    gaagaaAGTTGAGATCCCGGGTGTTGCGACTACCGCATCACCAAGTTCTGAAGTCGGTCGCTTCTTTGAAACCCAGTCATCAAAAGGCGGGCGTTACACCGTCACGCTCCCGGATGGCACTAAAGTCGAAGAACTGAACAAAGCGACCg                                                                                  >  2:4757969/1‑149 (MQ=255)
                                                                      agaaAGTTGAGATCCCGGGTGTTGCGACTACCGCATCACCAAGTTCTGAAGTCGGTCGCTTCTTTGAAAACCAGTCATCAAAATGCGGGCGTTACACCGTCACGCTCCCGGATGGCACTAAAGTCGAATAACTGAACAAAGCGACCCCa                                                                                >  1:1893343/1‑149 (MQ=255)
                                                                           gTTGAGATCCCGGGTGTTGCGACTACCGCATCACCAAGTTCTGAAGTCGGTCGCTTCTTTGAAACCCAGTCATCAAAAGGCGGGCGTTACACCGTCACGCTCCCGGATGGCACTAAAGTCGAAGAACTGAACAAAGCGACCGCAGCgaa                                                                           >  2:3223217/1‑148 (MQ=255)
                                                                            ttGAGATCCCGGGTGTTGCGACTACCGCATCACCAAGTTCTGAAGTCGGTCGCTTCTTTTAAAACCACTAATCAAAATGCGGGGGTTAAACCCTCACCCACCCGGATGGCACAAAAGTAGAAGAAAAGAACAAAACGACCGCACCgctg                                                                          >  1:1773548/1‑149 (MQ=255)
                                                                               agaTCCGGGGTGTTGCGACTACCGAATCACAATGTTCTGATGTCGGTCGATTATTTGAAACCCAGTCTTCAATCGGGGGGGTTTACACCGTCACGCTCCGGGTTGGCACTAAATTCTAAGAACTTAACAAAGCGACCGCAGCGatgatg                                                                       <  2:4016961/149‑1 (MQ=255)
                                                                                 aTCCCGGGTGTTGCGACTACCGCATCACCAAGTTCTGAAGTCGGTCGCTTCTTTGAAACCCAGTGATCAAAAGGCGGGCGTTACACCCTCACGCTCCCGGATGGCACTAAAGTCGAAGAACTGAACAAAGCGCCCCCACCGAAGATGGt                                                                     >  1:3559428/1‑149 (MQ=255)
                                                                                    ccGGGTGTGGCTACTACCGCAAAACATAGTTCTGAAGTCGGTCTATTCTTTGAAACCCAGTCATCAAATGGGGGTCTTTACACCGTCACGCTCCCGGTTGCAACAAATGTCGAATAACTTAAAAAAGCGACCGCAGCGATGATGGTccc                                                                  <  2:213153/149‑1 (MQ=255)
                                                                                      gggTGTTGCGACTACCGCATCACCAAGTTCTGATGTCGGTCGCTTCTTTGAAACCCAGTCATCAAAAGGCGGGCGTTACACCGTCACGCTCCCGTATGGCACTAAAGTCGAAGAACTGAACAAAGCGACCGCAGCGATGATGGTCCCGt                                                                <  2:1254410/149‑1 (MQ=255)
                                                                                            tGCGACTACCGCATCACCATGTTCTGACGTCGGTCGCTTCTTTGAAACCCAGTCATCAAATGGCGGGCGTTACACCGACACGCTCCCGTATGGCACTAAAGTCGAAGAACTGAACAAAGCGACCGCAGCGATGATGGTCCCGTTCGACa                                                          <  2:387269/149‑1 (MQ=255)
                                                                                              ctaCTACCGCATCAAAATGTACTTAAGTCGGTCTTTACTTTTAAACCCAGAAAAAAAATGGCGGGCGTAAAACCGTAACGCTCCGGTTTGGCAATAAATTCTAACAACTTAAAAAAGCGACCGCAGCGATGATGGTCCCGTTCGACAGc                                                        <  1:872354/147‑1 (MQ=255)
                                                                                                aCTACCGCATCACCAAGTTCTGAAGTCGGTCGCTTCTTTGAAACCCACTCCGCAAAAGGCGGGCGTTACACCGTCCCGCTCCCGGCTGGCACGAAAGACGCAGAACGGAACAAACCGACCGCAGCGATGATGGTCCCGTTCGACAGCaa                                                      >  2:4355329/1‑148 (MQ=255)
                                                                                                 cTACCGCATCACCAAGTTCTGAAGTCGGTCGCTTCTTTGAAAACCAGTCATCAAAAGGCGGGCGTTACACCGTCACGCTCCCGGATGGCACTAAAGTCGAATAAATGAACAAAGCGACCGCAGCGATGATGGTCCCGTTCGACAGCATc                                                     >  1:2431687/1‑149 (MQ=255)
                                                                                                  tACCGCATCACCAAGTTCTGAAGTCGGTCGCTTCTTTGAAACCCAGTCAACAAAAGGCGGGCGTTACACCGTCACCCTCCCGGATGGCACAAAAGTCGAAGAACTGAACAAAGCGACCGCAGCGATGATTGTCCCGGTCGCCCCCATCa                                                    >  1:4999874/1‑149 (MQ=255)
                                                                                                   aCCGCATCACCAAGTTCTGAAGTCGGTCGCTTCTTTGAAACCCAGTCATAAAAAGGCGGGCGTTACACCGTCACGCTCCCGGATGGCACTAAAGTCGAAGAACTGAACAAAGCGACCCCAGCGATGATGGTCCCGTTCGACAGCATCaa                                                   >  1:2088841/1‑149 (MQ=255)
                                                                                                    ccGCATCCCCAAGTTCTGATGTCGGTCGCTTCTTTGAAACCCAGTCATCAAAAGGCGGGCGTTACACCTTCACGCTCCCGGATGGCACTAACGTCGAATAACTTAACAAAGCGACCGCAGCGATGATGGTCCCGTTCGACAGCATCaaa                                                  <  2:2378776/149‑1 (MQ=255)
                                                                                                    ccGCATCACCATGTTCTTATGGCGGGCGCTTATTTGAAACCCAGTCATCAAATGGCGGGCGTTACACCGTCACGCGCCGGTTTGGCACTAAAGTCGAAGAACTGACCAACGCGACCGCAGCGATGATGGTCCCGTTCGACAGCATCaaa                                                  <  2:1442089/149‑1 (MQ=255)
                                                                                                      gCATCACAATGTTCTGAAGTCGGTCGCTTCTTTTAAACCCAGTCAACAAATGGCGGGCGTAACACCGTCACGCTCCCGGATGGCACTAAAGTCGAAGAACTGAAAAAAGCGACCGCAGCGATGATGGTCCCGTTCGACAGCATCAAAtt                                                <  2:1609179/149‑1 (MQ=255)
                                                                                                         tCACCAAGTTGTTATGTCGGTCGCTTCTTTTAAACCCAGTAATCAAATGGCGGGCGTTACACCGTCACGCTCCCGTTTGGCACTAAAGTCGAACAACTGAACAACGCGACCGCAGCGATGATGGTCCCGTTCGACAGCATCAAATtctc                                             <  2:191570/149‑1 (MQ=255)
                                                                                                         tCACCAAGTTCTGAAGTCGGTCGCTTATTTGAAACCCAGTCATCAAAAGGCGGGCGTTACACCGTCACGCTCCCGGATGGCACTAAAGTCGAATAACTGAACAAAGCGACCGCAGCGATGATGGTCCCGTTCGACAGCATCAAATtctc                                             <  2:2782834/149‑1 (MQ=255)
                                                                                                           agtttgTTGTGATGTCGGTCGGTTTTTTTAAAGCCAGTCTTAATATGGGGGGGGTTACACCGTCAGGGTCCGGTTTGGAACTTATTTCTAAGAACTGACCACCGCGACCGCAGCGATGATGGTCCCGTTCGACAGCATCAAATTCTCTg                                           <  2:5050289/144‑1 (MQ=255)
                                                                                                             cAAGTTCTTAAGTCGGTCGCTTCTTTGAAACCCAGTCATCAAAAGGCGGGCGTTACACCGTCACGCTCCCGTATGGCACTAAAGTCGAAGAACTGAACAAAGCGACCGCAGCGATGATGGTCCCGTTCGACAGCATCAAATTCTCTGGc                                         <  2:258818/149‑1 (MQ=255)
                                                                                                             cAAGTTATTAAGTCGGTCTCTTTTTTTAAACCCCGTCATCAATTGGCGGGCGTTACACCGTCACGCTCCCGGATGGCAATAACTGCGAACACCTCAACACCGCGACCGCAGCGATGATGGTCCCGTTCGACAGCATCAAATTCTCTGGc                                         <  2:9278/149‑1 (MQ=255)
                                                                                                              aaGTTCTGAAGTCGGTCGCTTCTTTGAAACCCAGTCATCAAAATGCGGGCGTTACACCGTCACGCTCCCGGATGGCACTAAAGTCGAAGAACTGAACAAAGCGACCGCAGCGATGATGGTCCCGGTCGACAGCATCAAATTCTCTGGCa                                        >  2:3559633/1‑149 (MQ=255)
                                                                                                                tttCTGAAGTCGGTCGCTTCTTTGAAACCCAGTCATCAAAAGGCGGGCGTTACACCGTCACGCTCCCGGATGGCACTAAAGTCGAAGAACTTAACAAAGCGACCGCAGCGATGATGGTCCCGTTCGACAGCATCAAATTCTCTGGCAAc                                      <  2:3175036/148‑1 (MQ=255)
                                                                                                                gTTCTGAAGTCGGTCGCTTCTTTGAAACCCAGTCATCAAATGGCGGGCTTTACACCTTCACGCTCCGGTATGGCACAAAAGTCTAACAACTTAACAACGCGACCGCAGCGATGATGGTCCCGTTCGACAGCATCAAATTCTCTGGCAAc                                      <  2:4950622/149‑1 (MQ=255)
                                                                                                                gTTCTGAAGTCGGTCGCTTCTTTGAAACCCAGTCATCAAAAGGCGGGCGTTACACCGTCACGCTCCCGGATGGCACTAAAGTCGACGAACTGAACAAAGCGACCGCAGCGATGATGGTCCCGTTCGACAGCATCAAATTCTCTGGCAAc                                      <  2:4217911/149‑1 (MQ=255)
                                                                                                                 ttCTGACGTGGGTCGTTTATTTTAAACCCAGTAATCAAACGGCGGGGTTTCCACCGACACGCCCCGGTTTGGCACTAAAGTCGAATAACTTAACAAAGCGACCGCAGCGATGATGGTCCCGTTCGACAGCATCAAATTCTCTGGCAACt                                     <  1:3768960/149‑1 (MQ=255)
                                                                                                                 ttCTGAAGTCGGTCGCTTCTTTGAACCCCCGTCATCAAAAGGCGGGCGTTACACCGTCACGCTCCCGGATGGCACTAAAGTCGAAGAACTGAACAAAGCGACCGCAGCGATGATGGTCCCGTTCGACAGCATCAAATTCTCTGGCAACt                                     <  2:4710964/149‑1 (MQ=255)
                                                                                                                  tCTGAAGTCGGTCGCTTCTTTGAAACCCAGTCATAAAAAGGCGGGCGTTACCCCGTCACGCGCCCGGATGGCACTAAAGTCGAAGAACTGAACAAAGCGACCGCAGCGATGATGGTCCCGTTCGACAGCATCAAATTCTCTGGCAACTa                                    <  2:2821017/149‑1 (MQ=255)
                                                                                                                      aaGTCGGTCGCGTCTTTGAAACCCAGTCATCAAAAGGCGGGCGTTAAACCGGCACCCTCCCCGATGGCCCTAAATTCGAAGAACTGAAAAAATCGACCGCAGCGATTATGGTCCCGGTCGACAGCATCAAATTCTCTGGCAAAAACGGc                                >  2:2178968/1‑149 (MQ=255)
                                                                                                                        ggggggcgCTTCTTTTAACCCCTGTCATAAAAAGGGGGGCGTGACACCGGCACGCGCCCGGATGGAACTAATGTCGACGAACTTACCAACGCGACCGCAGCGATGATGGTCCCGTTCGACAGCATCAAATTCTCTGGCAACTACGGCaa                              <  2:2595171/143‑1 (MQ=255)
                                                                                                                          gggtcgCTTATTTGAAACCATGTCATCAAACGGCGGGGTTTACACCTTCACGCTCCGGGATGGCACTAAAGTCGACTAACTTAACAAAGCGACCGCAGCGATGATGGTCCCGTTCGACAGCATCAAATTCTCTGGCAACTACGGCAACa                            <  1:3017518/148‑1 (MQ=255)
                                                                                                                            gtcgCTTATTTTAAACCCATTCATAAATTGGGGGGGTTTACACCGTCACGCGCCCGTATGGCAATAAAGTCGAAGAACTTAACAACGCGACCGCAGCGATGATGGTCCCGTTCGACAGCATCAAATTCTCTGGCAACTACGGCAACATg                          <  2:4842921/149‑1 (MQ=255)
                                                                                                                             tggattcttTTAACCCCTGTCATAAAATGGCGGGCTTTACCCCGGCACGCTCCGGGATGGAAATAATGTCGAACAACTTAAAAACGCGACCGCAGCGATGATGGTCCCGTTCGACAGCATCAAATTCTCTGGCAACTACGGCAACATGa                         <  1:4085383/145‑1 (MQ=255)
                                                                                                                              cgCTTCTTTGAAACCCAGTCATCAAAAGGCGGGCGTTACACCGTCACGCTCCCGGATGGCACTAAAGTCGAAGAACTGAACAAAGCGACCGCAGCGATGATGGTCCCGTTCGACAGCATCAAATTCTCTGGCAACTACGGCAACATGAc                        >  2:861627/1‑149 (MQ=255)
                                                                                                                              cgCTTCTTTGAAACCCAGTCATCAAAAGGCGGGCGTTACACCGTCACGCTCCCGGATGGCACTAAAGTCGAAGAACTGAACAAAGCGACCGCAGCGATGATGGTCCCGTTCGACAGCATCAAATTCTCTGGCAACTACGGCAACATGAc                        >  1:1165583/1‑149 (MQ=255)
                                                                                                                                cttcttTGAAACCCAGTCATCAAAAGGCGGGCGTTACACCGTCACGCTCCCGGATGGCACTAAAGTCGAAGAACTGAACAAAGCGACCGCAGCGATGATGGTCCCGTTCGACAGCATCAAATTCTCTGGCAACTACGGCAACATGACCg                      >  1:1515941/1‑149 (MQ=255)
                                                                                                                                cttcttTGAAACCCAGTCATCAAAAGGCGGGCGTTACACCGTCACGCTCCCGGATGGCACTAAAGTCGAAGAACTGAACAAAGCGACCGCAGCGATGATGGTCCCGTTCGACAGCATCAAATTCTCTGGCAACTACGGCAACATGACCg                      >  1:440898/1‑149 (MQ=255)
                                                                                                                                  tcttTGAAACCCAGTCATCAAATGGCGGGGGTTACACCGTCACGCTCCGGTATGGCACTAAAGTCGAAGAACTTAACAAAGCGACCGCAGCGATGATGGTCCCGTTCGACAGCATCAAATTCTCTGGCAACTACGGCAACATGACCGaa                    <  1:5156456/149‑1 (MQ=255)
                                                                                                                                  tcttTGAAACCCAGTCATCAAAAGGCGGGCGTTACACCGTCACGCTCCCGGATGGCACTAAAGTCGAAGAACTGAACAAAGCGACCGCAGCGATGATGGTCCCGTTCGACAGCATCAAATTCTCTGGCAACTACGGCAACATGACCGaa                    >  2:723998/1‑149 (MQ=255)
                                                                                                                                     ttcaaccccAGTAAAAAAAGGGGGGGCTTAACACCGTCCCGCGCCGGGTTGCCACTAACGTCCAAGAACTGAACAAAGCGACCGCAGCGATGATGGTCCCGTTCGACAGCATCAAATTCTCTGGCAACTACGGCAACATGACCGAAGtc                 <  1:610239/143‑1 (MQ=255)
                                                                                                                                       taaaCCCAGTCATAAAATGGGGGGCGTTACACCGACACGCGCCCGGTTGGACCTAACGTCGAACAACTTAACAAAGCGACCGCAGCGATGATGGTCCCGTTCGACAGCATCAAATTCTCTGGCAACTACGGCAACATGACCGAAGtctc               <  1:741621/148‑1 (MQ=255)
                                                                                                                                       gAACCCCATACATCAACGGGGGGGCTTAACACCGCCCCGCTCCCGGTTGCCACAAACGTCCAATAACTGAACAACGCGACCGCAGCGATGATGGTCCCGTTCGACAGCATCAAATTCTCTGGCAACTACGGCAACATGACCGAAGtctc               <  1:218588/149‑1 (MQ=255)
                                                                                                                                         aaCCCAGTCATCAAAAGGCGGGCGTTACACCGACACGCTCCCGTATGGCACTAAAGTCGAAGAACTGAACAAAGCGACCGCAGCGATGATGGTCCCGTTCGACAGCATCAAATTCTCTGGCAACTACGGCAACATGACCGAAGTCTCCt             <  2:1638125/149‑1 (MQ=255)
                                                                                                                                           cccAGTCATCAAACGGCGGGCGTTACACCGTCACGCTCCCGGATGGCACTAAAGTCTAAGAACTGAACAAAGCGACCGCAGCGATGATGGTCCCGTTCGACAGCATCAAATTCTCTGGCAACTACGGCAACATGACCGAAGTCTCCTAt           <  2:680899/149‑1 (MQ=255)
                                                                                                                                           cccAGTCATCAAAAGGCGGGCGTTACACCGTCACGCTCCCGGATGGCACTAAAGTCGAATAACTGAACAACGCGACCGCAGCGATGATGGTCCCGTTCGACAGCATCAAATTCTCTGGCAACTACGGCAACATGACCGAAGTCTCCTAt           <  2:3745172/149‑1 (MQ=255)
                                                                                                                                            ccAGTCATCAAAAGGCGGGCGTTACACCGTCACGCTCCCGTATGGCAATAATGTCGAAGAACTTAACAACGCGACCGCAGCGATGATGGTCCCGTTCGACAGCATCAAATTCTCTGGCAACTACGGCAACATGACCGAAGTCTCCTATc          <  2:2289202/149‑1 (MQ=255)
                                                                                                                                            ccAGTCATCAAAAGGCGGGCGTTACACCGTCACGCTCCCGGATGGCACTAAAGTCGAAGAACTGAACAAAGCGACCGCAGCGATGATGGTCCCGTTCGACAGCATCAAATTCTCTGGCAACTACGGCAACATGACCGAAGTCTCCTATc          >  2:1131056/1‑149 (MQ=255)
                                                                                                                                                tcatcaAAAGGCGGGCGTTACACCGTCACGCTCCCGGATGGCACTAAAGTCGAAGAACTGAACAAAGCGACCGCAGCGATGATGGTCCCGTTCGACAGCATCAAATTCTCTGGCAACTACGGCAACATGACCGAAGTCTCCTATCAGGt      >  1:2881594/1‑149 (MQ=255)
                                                                                                                                                 catcaAAAGGCGGGCGTTACACCGTCACGCTCCCGGATGGCCCTAAAGTCGACTAACTGAACACAGCGACCGCAGCGATGATGGTCCCGTTCGACAGCATCAAATTCTCTGGCAACTACGGCAACATGACCGAAGTCTCCTATCAGGtt     <  2:3250305/149‑1 (MQ=255)
                                                                                                                                                 catcaAAAGGCGGGCGTTACACCGTCACGCTCCCGGATGGCACTAAAGTCGAAGAACTGAACAAAGCGACCGCAGCGATGATGGTCCCGTTCGACAGCATCAAATTCTCTGGCAACTACGGCAACATGACCGAAGTCTCCTATCAGGtt     >  1:340097/1‑149 (MQ=255)
                                                                                                                                                   tcaAAAGGCGGGCGTTACACCGTCACGCTCCCGGATGGCACTAAAGTCGAAGAACTGAACAAAGCGACCGCAGCGATGATGGTCCCGTTCGACAGCATCAAATTCTCTGGCAACTACGGCAACATGACCGAAGTCTCCTATCAGGTTGc   >  2:4950025/1‑149 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
AGCCCGGATGTGATCCCGGCAGATTCCGAAGCAGGACGTGCAGCTCTGGCTGCCGGTGGCGAAGCCGCGAAGAAAGTTGAGATCCCGGGTGTTGCGACTACCGCATCACCAAGTTCTGAAGTCGGTCGCTTCTTTGAAACCCAGTCATCAAAAGGCGGGCGTTACACCGTCACGCTCCCGGATGGCACTAAAGTCGAAGAACTGAACAAAGCGACCGCAGCGATGATGGTCCCGTTCGACAGCATCAAATTCTCTGGCAACTACGGCAACATGACCGAAGTCTCCTATCAGGTTGCG  >  CP009273/1673233‑1673529

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑