Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,701,282 A→C 5.7% Q36P (CAG→CCG)  rsxC → SoxR iron‑sulfur cluster reduction factor component; putative membrane‑associated NADH oxidoreductase of electron transport complex

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,701,2820AC 5.7% 634.6 / 11.7 254Q36P (CAG→CCG) rsxCSoxR iron‑sulfur cluster reduction factor component; putative membrane‑associated NADH oxidoreductase of electron transport complex
Reads supporting (aligned to +/- strand):  ref base A (136/97);  new base C (19/2);  total (155/99)
Fisher's exact test for biased strand distribution p-value = 4.02e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 256 total aligned reads displayed.

ATCTGAACACCATTCCCGTGCGTATCATTCCCGTGGAACACCATGCTTAAGTTATTCTCTGCATTCAGAAAAAATAAAATCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTGGCGCTGAAGGTGAGTTGTGCGTTAGCGTCGGCGATAAAGTATTGCGCGGCCAGCCGCTTACCCGTGGTCGCGGCAAAATGCTGCCTGTTCACGCGCCCACC  >  CP009273/1701134‑1701430
                                                                                                                                                    |                                                                                                                                                    
 tCTGAACACCATTCCCGTGCGTATCATTCCCGTGGAACACCATGCTTAAGTTATTCTCTGCATTCAGAAAAAATAAAATCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAg                                                                                                                                                     <  2:3121716/149‑1 (MQ=255)
      acacCATTCCCGTGCGTATCATTCCCGTGGAACACCATGCTTAAGTTATTCTCTGCATTCAGAAAAAATAAAATCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTAcc                                                                                                                                                >  2:715089/1‑149 (MQ=255)
      acacCATTCCCGTGCGTATCATTCCCGTGGAACACCATGCTTAAGTTATTCTCTGCATTCAGAAAAAATAAAATCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTAcc                                                                                                                                                >  2:4241416/1‑149 (MQ=255)
       cacCATTCCCGTGCGTATCATTCCCGTGGAACACCATGCTTAAGTTATTCTCTGCATTCAGAAAAAATAAAATCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTAccc                                                                                                                                               >  2:3646120/1‑149 (MQ=255)
       cacCATTCCCGTGCGTATCATTCCCGTGGAACACCATGCTTAAGTTATTCTCTGCATTCAGAAAAAATAAAATCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTAccc                                                                                                                                               >  2:1598368/1‑149 (MQ=255)
        acCATTCCCGTGCGTATCATTCCCGTGGAACACCATGCTTAAGTTATTCTCTGCATTCAGAAAAAATAAAATCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTAcccc                                                                                                                                              <  1:2674299/149‑1 (MQ=255)
         ccATTCCCGTGCGTATCATTCCCGTGGAACACCATGCTTAAGTTATTCTCTGCATTCAGAAAAAATAAAATCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCt                                                                                                                                             <  1:1136145/149‑1 (MQ=255)
         ccATTCCCGTGCGTATCATTCCCGTGGAACACCATGCTTAAGTTATTCTCTGCATTCAGAAAAAATAAAATCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCt                                                                                                                                             <  1:3763078/149‑1 (MQ=255)
         ccATTCCCGTGCGTATCATTCCCGTGGAACACCATGCTTAAGTTATTCTCTGCATTCAGAAAAAATAAAATCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCt                                                                                                                                             <  1:1837628/149‑1 (MQ=255)
         ccATTCCCGTGCGTATCATTCCCGTGGAACACCATGCTTAAGTTATTCTCTGCATTCAGAAAAAATAAAATCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCt                                                                                                                                             >  2:1807422/1‑149 (MQ=255)
         ccATTCCCGTGCGTATCATTCCCGTGGAACACCATGCTTAAGTTATTCTCTGAATTCATAAAAAATAAAATCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCt                                                                                                                                             <  1:998697/149‑1 (MQ=255)
           aTTCCCGTGCGTATCATTCCCGTGGAACACCATGATTAAGTTATTCTCTGCATTCAGAAAAAATAAAATCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCTgg                                                                                                                                           <  2:4165114/149‑1 (MQ=255)
              cccGTGCGTATCATTCCCGTGGAACACCATGCTTAAGTTATTCTCTGCATTCAGAAAAAATAAAATCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCCGGTACCCCTGgcgc                                                                                                                                        >  2:1422274/1‑149 (MQ=255)
               ccGTGCGTATCATTCCCGTGGAACACCATGCTTAAGTTATTCTCTGCATTCAGAAAAAATAAAATCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCa                                                                                                                                       <  2:1588250/149‑1 (MQ=255)
                cGTGCGTATCATTCCCGTGGAACACCATGCTTAAGTTATTCTCTGCATTCAGAAAAAATAAAATCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCAGgcgccg                                                                                                                                      >  1:4091672/1‑147 (MQ=255)
                 gTGCGTATCATTCCCGTGGAACACCATGCTTAAGTTATTCTCTGCATTCAGAAAAAATAAAATCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCAGGCGCAGc                                                                                                                                     >  1:2786572/1‑149 (MQ=255)
                   gcttATCATTCCCGTGTAACACCATGCTTAAGTTATTCTCTGAATTCAGAAAAAATAAAATCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGt                                                                                                                                   <  1:3331606/146‑1 (MQ=255)
                     ttATCTTTCCCGTGTAACACCATGATTAAGTTATTCTATGAATTCAGAAAAAATAAAATCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGttt                                                                                                                                 <  1:723906/148‑1 (MQ=255)
                     gTATCATTCCCGGGGAACACCTTGCTTAAGTTATTCTCTGCATTCAGAAAAAATAAAATCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGttt                                                                                                                                 <  2:4525973/149‑1 (MQ=255)
                        tCATTCCCGTGGAACACCATGCTTAAGTTATTCTCTGCATTCAGAAAAAATAAAATCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGTTTTGt                                                                                                                              >  2:2098674/1‑149 (MQ=255)
                        tCATTCCCGTGGAACACCATGCTTAAGTTATTCTCTGCATTCAGAAAAAATAAAATCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGTTTTGt                                                                                                                              >  2:2425914/1‑149 (MQ=255)
                        tCATTCCCGTGGAACACCATGCTTAAGTTATTCTCTGCATTCAGAAAAAATAAAATCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGGTTTGt                                                                                                                              >  2:3057542/1‑149 (MQ=255)
                        tCATTCCCGTGGAACACCATGCTTAAGTTATTCTCTGCATTCAGAAAAAATAAAATCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCGGGCGCCGCGtgttgt                                                                                                                              >  1:3562968/1‑149 (MQ=255)
                         cagtCCCGTGGAACACCATGCTTAAGTTATTCTCTGCATTCAGAAAAAATAAAATCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGTTTTGtt                                                                                                                             <  2:5182408/146‑1 (MQ=255)
                          aTTCCCGTGGAACACCATGCTTAAGTTATTCTCTGCATTCAGAAAAAATAAAATCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGGACCCCTGGCGCAGCGTTTTGTTa                                                                                                                            >  2:176129/1‑149 (MQ=255)
                           ttCCCGTGGAACACCATGCTTAAGTTATTCTCTGCATTCAGAAAAAATAAAATCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGTTTTGTTAt                                                                                                                           >  1:3025156/1‑149 (MQ=255)
                            tCCCGTGGAACACCATGCTTAAGTTATTCTCTGCATTCAGAAAAAATAAAATCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTAACCCAGGCGCAGCGTTTTGTTAtt                                                                                                                          >  1:747515/1‑149 (MQ=255)
                               cGTGGAACACCATGCTTAAGTTATTCTCTGCATTCAGAAAAAATAAAATCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGTTTTGTTATTCCa                                                                                                                       <  1:1266593/149‑1 (MQ=255)
                                   gAACACCATGCTTAAGTTATTCTCTGCATTCAGAAAAAATAAAATCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCCGGTACCCCGGGCGCAGCGTTTTGTTATTACACTGa                                                                                                                   >  1:4479694/1‑149 (MQ=255)
                                     acacCATGCTTAATTTATTCTCTGCATTCAGAAAAAATAAAATCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGTTTTGTTATTCCACTGaaa                                                                                                                 <  1:788005/149‑1 (MQ=255)
                                        ccATGCTTAAGTTATTCTCTGCATTCAGAAAAAATAAAATCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCGGGCGCAGCGTTTTGTTATTACCCCGAAACAg                                                                                                              >  2:4421027/1‑149 (MQ=255)
                                         cATGCTTAAGTTATTCTCTGCATTCAGAAAAAATAAAATCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGc                                                                                                             >  2:5083153/1‑149 (MQ=255)
                                          aTGCGTAAGTTATTCTCTGCATTCAGAAAAAATAAAATCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCa                                                                                                            <  2:1184433/149‑1 (MQ=255)
                                           tGCTTAAGTTATTCTCTGCATTCAGAAAAAATAAAATCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGTTTTGGTATTCCACTGAAACAGCat                                                                                                           >  2:2487759/1‑149 (MQ=255)
                                           tGCTTAAGTTATTCTCTGCATTCAGAAAAAATAAAATCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGGTTTGTTATTGCCCTGAAACAGCat                                                                                                           >  1:5048975/1‑149 (MQ=255)
                                            tcTTAAGTTATTCTCTGAATTCATAAAAAATAAAATCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCata                                                                                                          <  1:3411146/148‑1 (MQ=255)
                                             cTTAAGTTATTCTCTGCATTCAGAAAAAATAAAATCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCCGGTACCCCTGGCGCAGCGGTTTGTTTTTCCACTGAAACAGCagat                                                                                                         >  1:2390770/1‑149 (MQ=255)
                                               tAAGTTATTCTCTGCATTCATAAAAAATAAAATCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTg                                                                                                       <  1:404074/149‑1 (MQ=255)
                                               tAAGTTATTCTCTGCATTCAGAAAAAATAAAATCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCCGGTACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTg                                                                                                       <  1:3895130/149‑1 (MQ=255)
                                               tAAGTTATTCTCTGCATACAGAAAAAATAAAATCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTg                                                                                                       <  1:2520146/149‑1 (MQ=255)
                                                aaGTTATTCTCTGCATTCAGAAAAAATAAAATCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTgg                                                                                                      <  1:3782569/149‑1 (MQ=255)
                                                 aGTTATTCTCTGCATTCAGAAAAAATAAAATCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCCGGTACCCCAGGCGCAGCGTTTTGTTATTCCACAGAAACAGCATATTGgc                                                                                                     >  1:336461/1‑149 (MQ=255)
                                                 aGTTATTCTCTGCATTCAGAAAAAATAAAATCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGTTTTGTTATTACACAGAAACAGCATATTGgc                                                                                                     >  1:1587030/1‑149 (MQ=255)
                                                  gTTATTCTCTGCATTCAGAAAAAATAAAATCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTGgcg                                                                                                    >  2:4665765/1‑149 (MQ=255)
                                                  gTTATTCTCTGCATTCAGAAAAAATAAAATCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCAGGCGCAGCGGTTTGTTATTCCCCTGAAACAGCATATTGgcg                                                                                                    >  1:4520534/1‑149 (MQ=255)
                                                   ttATTCTCTGCATTCAGAAAAAATAAAATCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAACCCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTGgcgc                                                                                                   <  2:2976305/149‑1 (MQ=255)
                                                   ttATTCTCTGCATTCAGAAAAAATAAAATCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCCTATTGgcgc                                                                                                   >  2:4412000/1‑149 (MQ=255)
                                                   ttATTCTCTGCATTCAGAAAAAATAAAATCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCAGATTGgcgc                                                                                                   >  2:5109901/1‑149 (MQ=255)
                                                      ttCTCTGCATTCAGAAAAAATAAAATCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATAGTGGCGCTgt                                                                                                >  2:4909937/1‑148 (MQ=255)
                                                       tctcTGCATTCAGAAAAAATAAAATCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTGGCGCTGaa                                                                                               >  1:2553161/1‑149 (MQ=255)
                                                           tGCATTCAGAAAAAATAAAATCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTGGCGCTGAAGGTg                                                                                           <  2:3748545/149‑1 (MQ=255)
                                                           tGCATTCAGAAAAAATAAAATCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTGGCGCTGAAGGTg                                                                                           <  2:3957291/149‑1 (MQ=255)
                                                           tGCATTCAGAAAAAATAAAATCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTGGCGCTGAAGGTg                                                                                           >  1:4242296/1‑149 (MQ=255)
                                                            gaaTTCAAAAAAAATAAAATCGGGTTTTTCAACGGCGGCATCCATCCACGGGAGATTAAAACCCAGTCCAACGGTACCCCCCTGCGCCAGGTACCCCGGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTGGCGCTGAAGGTGa                                                                                          <  1:911255/147‑1 (MQ=255)
                                                            gCATTCAGAAAAAATAAAATCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTGGCGCTGAAGGTGa                                                                                          >  1:4631291/1‑149 (MQ=255)
                                                                  aGAAAAAATAAAATCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCCGCGGTTTGTTATTCCACTGAAACAGCAAATTGGCGCTGAAGGTGAGTtgtg                                                                                    >  2:53460/1‑149 (MQ=255)
                                                                   gAAAAAATAAAATCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCAGGCGCCGCGTTTTGGTATTCCACAGAAACAGCATCTTGGCGCTGAAGGGGAGTTGTGc                                                                                   >  2:5114924/1‑149 (MQ=255)
                                                                     aaaaaTAAAATCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCACGTAACCCGGGCGCAGCGGGTTGTTATTACACTGAAACAGCCTAATGGCCCGGCAGGTGAGTTGTGCGt                                                                                 >  1:3323906/1‑149 (MQ=255)
                                                                      aaaataaaatCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCGGGCGCCGCGGTTTGTTATTCCACTGCAACAGCAGATTGGCGCGGAAGGTGGGTTGTGCggt                                                                                >  2:4343332/1‑147 (MQ=255)
                                                                         ataaaatCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCCGGTACCCCTGGCGCAGCGGGGTGTTATTACACAGAAACAGCCAATTGGCGCTGAAGGTGTGGTGTGCGGTAGc                                                                             >  1:3191105/1‑149 (MQ=255)
                                                                         ataaaatCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGTTTTGGTATTCCACTGAAACAGCATATTGGCGCTGAAGGTGAGTTGTGCGTTAGc                                                                             <  2:3328407/149‑1 (MQ=255)
                                                                          taaaatCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTGGCGCTGAAGGTGAGTTGTGCGTTAGCg                                                                            >  2:4010548/1‑149 (MQ=255)
                                                                            aaatCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTTCCCCTGGCGCCGCGGTTTGGTATTCCACTGAAACAGCATAGTGGCGCTGTAGGGGAGTTGTGGGTTAGCGTc                                                                          >  1:3277012/1‑149 (MQ=255)
                                                                             aatCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGGTTTGTTATTCCACTGAAAAAGCATATTGGCGCGGAAGGTGAGTTTTGCGTTAGCGTCg                                                                         >  1:171092/1‑149 (MQ=255)
                                                                                 tGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTGGCGCTGAAGGTGAGTTGTGCGTTAGCGTCGGCGa                                                                     >  2:4252997/1‑149 (MQ=255)
                                                                                 tGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTGGCGCTGAAGGTGAGTTGTGCGTTAGCGTCGGCGa                                                                     >  2:1274926/1‑149 (MQ=255)
                                                                                 ggggATTACAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTGGCGCTGAAGGTGAGTTGTGCGTTAGCGTCGGCGa                                                                     <  1:2491686/148‑1 (MQ=255)
                                                                                  gggATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTGGCGCTGAAGGTGAGTTGTGCGTTAGCGTCGGCGAt                                                                    <  2:115468/149‑1 (MQ=255)
                                                                                    gATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCAGAGTGGCGCTGAAGGTGAGTTGTGCGTTAGCGTCGGCGATaa                                                                  >  2:3335157/1‑149 (MQ=255)
                                                                                      tttCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCGGGCGCAGCGGTTTGTTATTGCACTGAAACAGCATATTGGCGCTGTAGGTGAGGTGTGCGTTAGCGTCGGCGATAAAg                                                                >  1:2379955/1‑149 (MQ=255)
                                                                                        tCACCGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTGGCGCTGAAGGTGAGTTGTGCGTTAGCGTCGGCGATAAAGTa                                                              <  2:2662842/149‑1 (MQ=255)
                                                                                           aCGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGGTTTGTTATTCCACTGAAACAGCATATTGGCGCTGAAGGTGAGTTGTGCGTTAGCGTCGGCGATAAAGTATTg                                                           >  2:4384818/1‑149 (MQ=255)
                                                                                            cggcggCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCCGGTACCCCCGGCGCCGCGGGTTGTTATTCCCCAGAAACAGCATATTTGCGCGGAAGGTGAGTTGTGCGGTAGCGTCGGCGATAAAGTATTgc                                                          >  1:665089/1‑149 (MQ=255)
                                                                                            cggcggCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGGTTTGTTATTCCACTGAAACAGCATATTGGCGGGGAAGGTGAGTTGTGCGTTAGCGTCGGCGATAAAGTATTgc                                                          >  1:2045245/1‑149 (MQ=255)
                                                                                             ggcggcATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTGGCGCTGAAGGTGAGTTGTGCGTTAGCGTCGGCGATAAAGTATTgcg                                                         <  2:337471/149‑1 (MQ=255)
                                                                                              gcggcATCCATCCACCGGAGATGAAAACCCAGTCCACCGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTGGCGCTGAAGGTGAGTTGTGCGTTAGCGTCGGCGATAAAGTATTgcgc                                                        <  2:3736513/149‑1 (MQ=255)
                                                                                              gcggcATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCCGGTACCCCAGGCGCAGCGTTTTGTTATTACACTGAAAAAGCAAATTTGCGCTGAACGTGTGTTGTGCGTTTGCGTCGGCGCTAAGGTATTtcgc                                                        >  2:3910802/1‑149 (MQ=255)
                                                                                              gcggcATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTGGCGCTGAAGGTGAGTTGTGCGTTAGCGTCGGCGATAAAGTATTgcgc                                                        <  2:1128695/149‑1 (MQ=255)
                                                                                                 gcATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGGTTTGTTATTCCACTGAAACAGCATATTGGCGCTGAAGGTGAGTTGTGCGTTAGCGTCGGCGATAAAGTATTGCGCGGc                                                     >  2:4400272/1‑149 (MQ=255)
                                                                                                   atccatccACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCAGGCGCAGCGTTTTGGTATTCCACTGAAACAGCATATTGGCGCTGAAGGTGAGTTTTGCGGTAGCGTCGGCGCTAAAGTATTGCGCGGCCa                                                   >  1:4283934/1‑149 (MQ=255)
                                                                                                   atccatccACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCACGTAACCCGGGCGCAGCGGTTTGTTTTTACACAGAAACAGCAGATTGGCGCGGTACGTGAGTTTTTGGTTAGCGTCGGCGCTAAAGTATTGCGCGGccc                                                   >  1:4029153/1‑148 (MQ=255)
                                                                                                        tccaCCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGGTTTGTTATTCCACTGAAACAGCATATTGGCGCGGAAGGTGAGTTGTGCGTTAGCGTCGGCGATAAAGTATTGCGCGGCCAGCCGc                                              >  1:1664298/1‑149 (MQ=255)
                                                                                                           aCCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTGGCGCTGAAGGTGAGTTGTGCGTTAGCGTCGGCGATAAAGGATTGCGCGGCCCGCCGCGTa                                           >  2:2865520/1‑149 (MQ=255)
                                                                                                            ccGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGTTTTTTGATTCCACTGAAACAGCATATTGGCGCTGAAGGTGGGTTGTGCGTTTGCGTCGGCGATAAAGTATTGCGCGGCCAGCCGCTTAc                                          >  2:3494492/1‑149 (MQ=255)
                                                                                                            ccGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCGGGCGCCGCGTGTTGTTATTCCACGGTAAAAGCAGATTGGCGGGGGAGGTGTGTTTTGGGTTTGCGTCGGCGATAAATTATTGCGCGGCCCGCCGCTTaa                                          >  1:4466994/1‑148 (MQ=255)
                                                                                                             cGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTGGCGCTGAAGGTGAGTTGTGCGTTAGCGTCGGCGATAAAGTATTGCGCGGCCAGCCGCTTAcc                                         <  2:185826/149‑1 (MQ=255)
                                                                                                              ggAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTGGCGCTGAAGGTGAGTTGTGCGTTAGCGCCGGCGATAAAGTATTGCGCGGCCAGCCGCTTAccc                                        <  2:3775860/149‑1 (MQ=255)
                                                                                                                 gaTGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCAGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTGGCGCGGAAGGTGAGTTGTGCGTTAGCGTCGGCGATAAAGTATTGCGCGGCCAGCCGCTTAccccag                                     >  1:4279245/1‑146 (MQ=255)
                                                                                                                   tGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTGGCGCTGAAGGTGAGTTGTGCGTTAGCGTCGGCGATAAAGTATTGCGCGGCCAGCCGCTTACCCGTGGt                                   >  2:2128846/1‑149 (MQ=255)
                                                                                                                    gAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTGGCGCTGAAGGTGAGTTGTGCGTTAGCGTCGGCGATAAAGTATTGCGCGGCCAGCCGCTTACCCGGGGTc                                  >  1:3881207/1‑149 (MQ=255)
                                                                                                                    gAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCAGGCGCAGCGTTTTGTTATTACACTGAAACAGCATATTGGCGCTGAAGGTGAGTTGTGGGTTAGCGTCGGCGATAAAGTATTGCGCGGCCAGCCGCTTACCCGAGGTc                                  >  1:4215384/1‑149 (MQ=255)
                                                                                                                       aaCCCAGTCCAACGGTACACCCCTGCGCCAGGGACCCCTGGCGCCGCGGTTTGTTTTTCCACAGAAACAGCCTATTTGCGCTGAAGGTGAGTTGTGCGGTAGCGTCGGCGATAAAGTATTGCGCGGCCCGCCGCATACCCCGGGGcgcg                               >  2:4825167/1‑149 (MQ=255)
                                                                                                                         cccAGTCCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTGGCGCTGAAGGTGAGTTGTGCGTTAGCGTCGGCGATAAAGTATTGCGCGGCCAGCCGCTTACCCGTGGTCGCGGc                             <  1:4594351/149‑1 (MQ=255)
                                                                                                                         cccAGTCCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGTTTTGTTATTACACTGAAACAGCATATTGGCGCTGAAGGTGAGTTGTGCGTTAGCGTCGGCGATAAAGTATTGCGCGGCCAGCCGCTTACCCGTGGTCGCGGc                             >  1:2086155/1‑149 (MQ=255)
                                                                                                                                ccaCGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTGGCGCTGAAGGTGAGTTGTGCGTTAGCGTCGGCGATAAAGTATTGCGCGGCCAGCCGCTTACCCGTGGTCGCGGCAAAAtgc                      <  1:3646120/147‑1 (MQ=255)
                                                                                                                                cAACGTTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTGGCGCTGAAGGTGAGTTGTGCGTTAGCGTCGGCGATAAAGTATTGCGCGGCCAGCCGCTTACCCGTGGTCGCGGCAAAAtgc                      <  1:3564606/149‑1 (MQ=255)
                                                                                                                                     gTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTGGCGCTGAAGGTGAGTTGTGCGTTAGCGTCGGCGATAAAGTATTGCGCGGCCAGCCGCTTACCCGTGGTCGCGGCAAAATGCTGCCt                 <  2:5081026/149‑1 (MQ=255)
                                                                                                                                       acacCCCTGCGCCAGGTACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTGGCGCTGAAGGTGAGTTGTGCGTTAGCGTCGGCGATAAAGTATTGCGCGGCCAGCCGCTTACCCGTGGTCGCGGCAAAATGCTGCCTGt               <  2:4242702/149‑1 (MQ=255)
                                                                                                                                             cggcgcCCGGTACCCCGGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTGGCGCTGAAGGTGAGTTGTGCGTTAGCGTCGGCGATAAAGTATTGCGCGGCCAGCCGCTTACCCGTGGTCGCGGCAAAATGCTGCCTGTTCAcgc         <  1:4791493/147‑1 (MQ=255)
                                                                                                                                                    aGGTACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTGGCGCTGAAGGTGAGTTGTGCGTTAGCGTCGGCGATAAAGTATTGCGCGGCCAGCCGCTTACCCGTGGTCGCGGCAAAATGCTGCCTGTTCACGCGCCCAcc  <  2:3892068/149‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
ATCTGAACACCATTCCCGTGCGTATCATTCCCGTGGAACACCATGCTTAAGTTATTCTCTGCATTCAGAAAAAATAAAATCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTGGCGCTGAAGGTGAGTTGTGCGTTAGCGTCGGCGATAAAGTATTGCGCGGCCAGCCGCTTACCCGTGGTCGCGGCAAAATGCTGCCTGTTCACGCGCCCACC  >  CP009273/1701134‑1701430

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑