Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,701,291 T→G 8.6% L39R (CTG→CGG)  rsxC → SoxR iron‑sulfur cluster reduction factor component; putative membrane‑associated NADH oxidoreductase of electron transport complex

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,701,2910TG 8.6% 517.7 / 2.6 243L39R (CTG→CGG) rsxCSoxR iron‑sulfur cluster reduction factor component; putative membrane‑associated NADH oxidoreductase of electron transport complex
Reads supporting (aligned to +/- strand):  ref base T (104/87);  new base G (22/4);  total (151/92)
Fisher's exact test for biased strand distribution p-value = 2.98e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 251 total aligned reads displayed.

CCATTCCCGTGCGTATCATTCCCGTGGAACACCATGCTTAAGTTATTCTCTGCATTCAGAAAAAATAAAATCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTGGCGCTGAAGGTGAGTTGTGCGTTAGCGTCGGCGATAAAGTATTGCGCGGCCAGCCGCTTACCCGTGGTCGCGGCAAAATGCTGCCTGTTCACGCGCCCACCTCGGGTACC  >  CP009273/1701143‑1701439
                                                                                                                                                    |                                                                                                                                                    
ccATTCCCGTGCGTATCATTCCCGTGGAACACCATGCTTAAGTTATTCTCTGCATTCAGAAAAAATAAAATCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCt                                                                                                                                                      <  1:1136145/149‑1 (MQ=255)
ccATTCCCGTGCGTATCATTCCCGTGGAACACCATGCTTAAGTTATTCTCTGCATTCAGAAAAAATAAAATCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCt                                                                                                                                                      <  1:3763078/149‑1 (MQ=255)
ccATTCCCGTGCGTATCATTCCCGTGGAACACCATGCTTAAGTTATTCTCTGCATTCAGAAAAAATAAAATCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCt                                                                                                                                                      >  2:1807422/1‑149 (MQ=255)
ccATTCCCGTGCGTATCATTCCCGTGGAACACCATGCTTAAGTTATTCTCTGAATTCATAAAAAATAAAATCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCt                                                                                                                                                      <  1:998697/149‑1 (MQ=255)
  agtCCCGTGCGTATCAGTCCCGTGGAACACCATGCTTAAGTTATTCTCTGCATTCAGAAAAAATAAAATCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCTgg                                                                                                                                                    <  2:4165173/147‑1 (MQ=255)
  aTTCCCGTGCGTATCATTCCCGTGGAACACCATGCTTAAGTTATTCTCTGCATTCAGAAAAAATAAAATCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCTgg                                                                                                                                                    >  2:2943260/1‑149 (MQ=255)
  aTTCCCGTGCGTATCATTCCCGTGGAACACCATGCTTAAGTTATTCTCTGCATTCAGAAAAAATAAAATCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCTgg                                                                                                                                                    >  2:1137792/1‑149 (MQ=255)
     cccGTGCGTATCATTCCCGTGGAACACCATGCTTAAGTTATTCTCTGCATTCAGAAAAAATAAAATCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCCGGTACCCCTGgcgc                                                                                                                                                 >  2:1422274/1‑149 (MQ=255)
      ccGTGCGTATCATTCCCGTGGAACACCATGCTTAAGTTATTCTCTGCATTCAGAAAAAATAAAATCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCa                                                                                                                                                <  2:1588250/149‑1 (MQ=255)
       cGTGCGTATCATTCCCGTGGAACACCATGCTTAAGTTATTCTCTGCATTCAGAAAAAATAAAATCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCGGGCGCAg                                                                                                                                               >  1:4088759/1‑149 (MQ=255)
        gTGCGTATCATTCCCGTGGAACACCATGCTTAAGTTATTCTCTGCATTCAGAAAAAATAAAATCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGc                                                                                                                                              <  1:3690268/149‑1 (MQ=255)
          gcttATCATTCCCGTGTAACACCATGCTTAAGTTATTCTCTGAATTCAGAAAAAATAAAATCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGt                                                                                                                                            <  1:3331606/146‑1 (MQ=255)
           cGTATCTTTCCCGTGTAACACCATGCTTAAGTTATTCACTGCTTTCAGAAAAAATAAAAGCTGGGATTACAACGGCGGCATCCATCCACCGGAGATGAAAACCATGTCCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGtt                                                                                                                                           <  1:3319897/149‑1 (MQ=255)
            ttATCTTTCCCGTGTAACACCATGATTAAGTTATTCTATGAATTCAGAAAAAATAAAATCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGttt                                                                                                                                          <  1:723906/148‑1 (MQ=255)
               tCATTCCCGTGGAACACCATGCTTAAGTTATTCTCTGCATTCAGAAAAAATAAAATCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGGTTTGt                                                                                                                                       >  2:3057542/1‑149 (MQ=255)
                cagtCCCGTGGAACACCATGCTTAAGTTATTCTCTGCATTCAGAAAAAATAAAATCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGTTTTGtt                                                                                                                                      <  2:5182408/146‑1 (MQ=255)
                 aTTCCCGTGGAACACCATGCTTAAGTTATTCTCTGCATTCAGAAAAAATAAAATCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGGACCCCTGGCGCAGCGTTTTGTTa                                                                                                                                     >  2:176129/1‑149 (MQ=255)
                   tCCCGTGGAACACCATGCTTAAGTTATTCTCTGCATTCAGAAAAAATAAAATCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGTTTTGTTAtt                                                                                                                                   >  1:747432/1‑149 (MQ=255)
                       gTGGAACACCATGCTTAAGTTATTCTCTGCATTCAGAAAAAATAAAATCTGGGTTTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGTTTTGTTATTCCAc                                                                                                                               <  2:3072741/149‑1 (MQ=255)
                         ggAACACCATGCTTAAGTTATTCTCTGCATTCAGAAAAAATAAAATCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCCGGTACCCCTGGCGCAGCGTTTTGTTATTacacag                                                                                                                             >  1:4222648/1‑147 (MQ=255)
                            acacCATGCTTAATTTATTCTCTGCATTCAGAAAAAATAAAATCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGTTTTGTTATTCCACTGaaa                                                                                                                          <  1:788005/149‑1 (MQ=255)
                               ccATGCTTAAGTTATTCTCTGCATTCAGAAAAAATAAAATCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCCGGTACCCCGGGCGCAGCGTTTTGTTATTCCACTGAAACAg                                                                                                                       >  2:4421022/1‑149 (MQ=255)
                                cATGCTTAAGTTATTCTCTGCATTCAGAAAAAATAAAATCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGc                                                                                                                      >  2:5083153/1‑149 (MQ=255)
                                cATGCTTAAGTTATTCTCTGCATTCAGAAAAAATAAAATCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGTGTTGTTATTCCACTGAAACAGc                                                                                                                      >  2:3980215/1‑149 (MQ=255)
                                 aTGCGTAAGTTATTCTCTGCATTCAGAAAAAATAAAATCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCa                                                                                                                     <  2:1184433/149‑1 (MQ=255)
                                  tGCTTAAGTTATTCTCTGCATTCAGAAAAAATAAAATCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGTTTTGGTATTCCACTGAAACAGCat                                                                                                                    >  2:2487759/1‑149 (MQ=255)
                                  tGCTTAAGTTATTCTCTGCATTCAGAAAAAATAAAATCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGGTTTGTTATTGCCCTGAAACAGCat                                                                                                                    >  1:5048975/1‑149 (MQ=255)
                                   tcTTAAGTTATTCTCTGAATTCATAAAAAATAAAATCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCata                                                                                                                   <  1:3411146/148‑1 (MQ=255)
                                    cTTAAGTTATTCTCTGCATTCAGAAAAAATAAAATCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCCGGTACCCCTGGCGCAGCGGTTTGTTTTTCCACTGAAACAGCagat                                                                                                                  >  1:2390770/1‑149 (MQ=255)
                                    cTTAAGTTATTCTCTGCATTCAGAAAAAATAAAATCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCCCCAGGTACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCatat                                                                                                                  >  1:2390339/1‑149 (MQ=255)
                                      tAAGTTATTCTCTGCATTCATAAAAAATAAAATCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTg                                                                                                                <  1:404074/149‑1 (MQ=255)
                                      tAAGTTATTCTCTGCATTCAGAAAAAATAAAATCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCCGGTACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTg                                                                                                                <  1:3895130/149‑1 (MQ=255)
                                      tAAGTTATTCTCTGCATACAGAAAAAATAAAATCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTg                                                                                                                <  1:2520146/149‑1 (MQ=255)
                                         gTTATTCTCTGCATTCAGAAAAAATAAAATCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTGgcg                                                                                                             >  2:4665765/1‑149 (MQ=255)
                                          ttATTCTCTGCATTCAGAAAAAATAAAATCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCAGATTGgcgc                                                                                                            >  2:5109901/1‑149 (MQ=255)
                                          ttATTCTCTGCATTCAGAAAAAATAAAATCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGTGTTGTTATTCCACTGAAACAGCCTATTGgcgc                                                                                                            >  2:4411870/1‑149 (MQ=255)
                                            aTTCTCTGCATTCAGAAAAAATAAAATCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCCGGTACCCCTGGCGCCGCGTTTTGTTATTCCACGGAAACAGCAGATGGgcgcgg                                                                                                          >  2:2337570/1‑147 (MQ=255)
                                            aTTCTCTGCATTCAGAAAAAATAAAATCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCAGATTGGCGCTg                                                                                                          >  2:4872478/1‑149 (MQ=255)
                                             ttCTCTGCATTCAGAAAAAATAAAATCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATAGTGGCGCTgt                                                                                                         >  2:4909937/1‑148 (MQ=255)
                                              tctcTGCATTCAGAAAAAATAAAATCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTGGCGCTGaa                                                                                                        >  1:2553168/1‑149 (MQ=255)
                                                  tGCATTCAGAAAAAATAAAATCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTGGCGCTGAAGGTg                                                                                                    >  1:4242296/1‑149 (MQ=255)
                                                  tGCATTCAGAAAAAATAAAATCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTGGCGCTGAAGGTg                                                                                                    <  2:3748545/149‑1 (MQ=255)
                                                   gCATTCAGAAAAAATAAAATCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCCGGGAACCCAGGCGCAGCGTGTTGTTTTTAAACGGAAACAGACAATTGTCGCTGAATGTGa                                                                                                   >  1:1742601/1‑149 (MQ=255)
                                                      ttCAGAAAAAATAAAATCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGTTGTGTTATTCCACTGAAACAGCATATTGGCGCTGAAGGTGAGtt                                                                                                >  2:730631/1‑149 (MQ=255)
                                                         aGAAAAAATAAAATCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCCGCGGTTTGTTATTCCACTGAAACAGCAAATTGGCGCTGAAGGTGAGTtgtg                                                                                             >  2:53460/1‑149 (MQ=255)
                                                         aGAAAAAATAAAATCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCAACTTGGCGCTGAAGGTGAGTtgtg                                                                                             >  2:2644660/1‑149 (MQ=255)
                                                          gAAAAAATAAAATCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCAGGCGCCGCGTTTTGGTATTCCACAGAAACAGCATCTTGGCGCTGAAGGGGAGTTGTGc                                                                                            >  2:5114924/1‑149 (MQ=255)
                                                            aaaaaTAAAATCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCAGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATCTTGGCGCTGAAGGTGAGTTTTGCGt                                                                                          >  1:4487051/1‑149 (MQ=255)
                                                              aaataaaatCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGGTTTGTTATTCCACTGAAACAGCATATTGGCGCGGAAGGTGAGTTGTGCGTTa                                                                                        >  2:1218960/1‑149 (MQ=255)
                                                               aataaaatCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTGGCGCTGAAGGTGAGTTGTGCGTTAg                                                                                       <  1:2867396/149‑1 (MQ=255)
                                                                ataaaatCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCCGGTACCCCTGGCGCAGCGGGGTGTTATTACACAGAAACAGCCAATTGGCGCTGAAGGTGTGGTGTGCGGTAGc                                                                                      >  1:3191105/1‑149 (MQ=255)
                                                                ataaaatCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGTTTTGGTATTCCACTGAAACAGCATATTGGCGCTGAAGGTGAGTTGTGCGTTAGc                                                                                      <  2:3328407/149‑1 (MQ=255)
                                                                   aaatCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTTCCCCTGGCGCCGCGGTTTGGTATTCCACTGAAACAGCATAGTGGCGCTGTAGGGGAGTTGTGGGTTAGCGTc                                                                                   >  1:3277012/1‑149 (MQ=255)
                                                                   aaatCGGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTGGCGCTGAAGGTGAGTTGTGCGTTAGCGTc                                                                                   <  1:2515214/149‑1 (MQ=255)
                                                                        tGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTGGCGCTGAAGGTGAGTTGTGCGTTAGCGTCGGCGa                                                                              >  2:4252997/1‑149 (MQ=255)
                                                                        tGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGTTTTGTTATTACACTGAAACAGCATATTGGCGCTGAAGGTGAGTTGTGCGTTAGCGTCGGCGa                                                                              >  1:3449553/1‑149 (MQ=255)
                                                                        tGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCACGTACCCCAGGCGCCGCGGTTTGTTTTTACACTGAAACAGCATATTGTCGCGGGAGGTGTGGTGTGGGGTTGGGTCggggc                                                                              >  1:3581466/1‑146 (MQ=255)
                                                                           gATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCAGAGTGGCGCTGAAGGTGAGTTGTGCGTTAGCGTCGGCGATaa                                                                           >  2:3335157/1‑149 (MQ=255)
                                                                           gATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCGGGCGCAGCGTGTTGGTATTCCACTGAAACAGCATATTGGCGCGGAAGGGGAGTTGTGCGGTAGCGTCGGCGATaa                                                                           >  1:3354895/1‑149 (MQ=255)
                                                                            aTTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGGTTTGTTATTCCACTGAAACAGCATATTGGCGCTGAAGGTGAGTTGTGCGTTAGCGTCGGCGATcaa                                                                          >  2:4149561/1‑149 (MQ=255)
                                                                             tttCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCCGGTACCCCAGGGGCCGCGGGTTGTTATTGCCCGGAAAAAGCATATTGGGGGTGGCGGTGTGTTGTGGGGTTGAGTCGGCgggtaag                                                                         >  1:899645/1‑143 (MQ=255)
                                                                               tCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGTTTGGTTATTCCACTGAAACAGCATATTGGCGCTGAAGGTGAGTTGTGCGTTAGCGTCGGCGAGAAAGTa                                                                       >  2:4656894/1‑149 (MQ=255)
                                                                                cAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGTTTTGTTATTCCACAGAAACAGCATATTGGCGCTGAAGGTGAGTTGTGCGTTAGCGTCGGCGATAAAGTAt                                                                      >  1:1702266/1‑149 (MQ=255)
                                                                                  aCGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCGGGCGCAGCGGTTTGGTATTCCACTGAAACAGCATATTGGCGCTGTAGGTGTGGTGTGCGGTAGCGTCGGCGCTAAAGTATTg                                                                    >  2:4385184/1‑149 (MQ=255)
                                                                                   cggcggCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGGTTTGTTATTCCACTGAAACAGCATATTGGCGGGGAAGGTGAGTTGTGCGTTAGCGTCGGCGATAAAGTATTgc                                                                   >  1:2045245/1‑149 (MQ=255)
                                                                                    ggcggcATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTGGCGCTGAAGGTGAGTTGTGCGTTAGCGTCGGCGATAAAGTATTgcg                                                                  <  2:337471/149‑1 (MQ=255)
                                                                                     gcggcATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTGGCGCTGAAGGTGAGTTGTGCGTTAGCGTCGGCGATAAAGTATTgcgc                                                                 <  2:1128695/149‑1 (MQ=255)
                                                                                          atccatccACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCGGGCGCAGCGGTTTGTTATTACACTGAAACAGCATATTTGCGCTGAAGGTGTGTTGTGCGTTATCGTCGGCGATAAATTATTTAGCGGCCa                                                            >  1:4009689/1‑149 (MQ=255)
                                                                                          atccatccACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCAGGCGCAGCGTTTTGGTATTCCACTGAAACAGCATATTGGCGCTGAAGGTGAGTTTTGCGGTAGCGTCGGCGCTAAAGTATTGCGCGGCCa                                                            >  1:4283934/1‑149 (MQ=255)
                                                                                             catccaCCGGAGATGACAACCCAGTCCACCGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGTTTGGTTAGTCCACTGACACAGCCTCTTGGCGCTGAAGGTGAGGGGGGCGTTAGCGTCGGCGCGAAAGTATTGCGCGGCCAGcc                                                         <  2:194756/149‑1 (MQ=255)
                                                                                              atccaCCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCCGGTAACCCAGGCGCAGCGTTTTGTTATTACACGGAAAAAACCAATTGGGGCGGAAGGTGAGTTGTGGGTTAGCGTCGGCGCTAAAAGATTTCGCGGCCCGccc                                                        >  1:4471512/1‑148 (MQ=255)
                                                                                              atccaCCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTAACCCGGGCGCACCGTTTTGTTATTACCCAGAAACAGCATATTGGCGCTGAAGGTGAGTTGTGCGTTAGCGTCGGCGATAAAGTATTTCGCGGCCAGCCg                                                        >  1:4572601/1‑149 (MQ=255)
                                                                                                  aCCGGAGATGAAAACCCTGTCCAACGGTACCCCCCTGCGCCAGGTCCCCCTGGCGAAGCGTTTTGTTATTCCACTGAAACAGCATATTGGCGCTGAAGGTGAGTTGTGCGTTAGCGTCGGCGATAAAGTATTGCGCGGCCAGCCGCTTa                                                    <  1:2674546/149‑1 (MQ=255)
                                                                                                  aCCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTGGCGCTGAAGGTGAGTTGTGCGTTAGCGTCGGCGATAAAGGATTGCGCGGCCCGCCGCGTa                                                    >  2:2865520/1‑149 (MQ=255)
                                                                                                   ccGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCCCGTACCCCCGGCGCAGCGTTTTGTTAAACCACTGAAACACCATATTGGCGCTGAAGGTGTGTTGTGCGTTAGCGTCGGCGCTCAACTCTTGCCCGGCCCGCCCCTTaa                                                   >  2:4360102/1‑148 (MQ=255)
                                                                                                   ccGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGTTTTTTGATTCCACTGAAACAGCATATTGGCGCTGAAGGTGGGTTGTGCGTTTGCGTCGGCGATAAAGTATTGCGCGGCCAGCCGCTTAc                                                   >  2:3494492/1‑149 (MQ=255)
                                                                                                    cGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTGGCGCTGAAGGTGAGTTGTGCGTTAGCGTCGGCGATAAAGTATTGCGCGGCCAGCCGCTTAcc                                                  <  2:185826/149‑1 (MQ=255)
                                                                                                     ggAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTGGCGCTGAAGGTGAGTTGTGCGTTAGCGCCGGCGATAAAGTATTGCGCGGCCAGCCGCTTAccc                                                 <  2:3775860/149‑1 (MQ=255)
                                                                                                        gaTGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCAGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTGGCGCGGAAGGTGAGTTGTGCGTTAGCGTCGGCGATAAAGTATTGCGCGGCCAGCCGCTTAccccag                                              >  1:4279245/1‑146 (MQ=255)
                                                                                                          tGAAAACCCAGTCCAACGGTACACCCCTGCGCCCCGTACCCCTGGCGCAGCGTTTTGTTTTTACAAAGAAAAAGCCTATTTGCGCTGAATGTTAGTTGTGGGTTAGCGTCGGCGATAAAGTATTTAGCGGCCAGCCCCTTTCCCCTGGt                                            >  1:1605369/1‑149 (MQ=255)
                                                                                                          tGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTGGCGCTGAAGGTGAGTTGTGCGTTAGCGTCGGCGATAAAGTATTGCGCGGCCAGCCGCTTACCCGTGGt                                            >  2:2128846/1‑149 (MQ=255)
                                                                                                           gAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTGGCGCTGAAGGTGAGTTGTGCGTTAGCGTCGGCGATAAAGTATTGCGCGGCCAGCCGCTTACCCGGGGTc                                           >  1:3881207/1‑149 (MQ=255)
                                                                                                                cccAGTCCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTGGCGCTGAAGGTGAGTTGTGCGTTAGCGTCGGCGATAAAGTATTGCGCGGCCAGCCGCTTACCCGTGGTCGCGGc                                      <  1:4595125/149‑1 (MQ=255)
                                                                                                                cccAGTCCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGTTTTGTTATTACACTGAAACAGCATATTGGCGCTGAAGGTGAGTTGTGCGTTAGCGTCGGCGATAAAGTATTGCGCGGCCAGCCGCTTACCCGTGGTCGCGGc                                      >  1:2086155/1‑149 (MQ=255)
                                                                                                                cccAGTCCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGGTTTGTTATTACACAGAAACAGCATATTGGCGCTGAAGGTGAGTTGTGCGTTAGCGTCGGCGATAAAGTATTGCGCGGCCAGCCGCTTACCCGTGGTCGCGGc                                      >  1:4695783/1‑149 (MQ=255)
                                                                                                                       cAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTGGCGCTGAAGGTGAGTTGTGCGTTAGCGTCGGCGATAAAGTATTGCGCGGCCAGCCGCTTACCCGTGGTCGCGGCAAAAtgc                               <  1:3564485/149‑1 (MQ=255)
                                                                                                                            gTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTGGCGCTGAAGGTGAGTTGTGCGTTAGCGTCGGCGATAAAGTATTGCGCGGCCAGCCGCTTACCCGTGGTCGCGGCAAAATGCTGCCt                          <  2:5081026/149‑1 (MQ=255)
                                                                                                                              acacCCCTGCGCCAGGTACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTGGCGCTGAAGGTGAGTTGTGCGTTAGCGTCGGCGATAAAGTATTGCGCGGCCAGCCGCTTACCCGTGGTCGCGGCAAAATGCTGCCTGt                        <  2:4242702/149‑1 (MQ=255)
                                                                                                                                 ccccTGCGCCAGGTACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTGGCGCAGAAGGTGAGTTGTGCGTTAGCGTCGGCGATAAAGTATTGCGCGGCCAGCCGCTTACCCGTGGTCGCGGCAAAATGCTGCCTCTTCa                     >  2:3915467/1‑149 (MQ=255)
                                                                                                                                  cccTGCGCCAGGTACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTGGCGCTGAAGGTGAGTTGTGCGTTAGCGTCGGCGATAAAGTATTGCGCGGCCAGCCGCTTACCCGTGGTCGCGGCAAAATGCTGCCTGTTCAc                    <  1:4804985/149‑1 (MQ=255)
                                                                                                                                       cgcCAGGTACCCCCGGCGCTGCGTTGTGTTTTTCCCCGGAAAGAGCATATTGGCGCTGAAGGTGAGGTGTGCGTGAGCGTCGGCGATACAGTATTGCGCGGCCAGCCGCTTACCCGTGGTCGCGGCACAATGCTGCCGGTTCACGCGcc               <  2:4472062/149‑1 (MQ=255)
                                                                                                                                           aGGTACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTGGCGCTGAAGGTGAGTTGTGCGTTAGCGTCGGCGATAAAGTATTGCGCGGCCAGCCGCTTACCCGTGGTCGCGGCAAAATGCTGCCTGTTCACGCGCCCAcc           <  2:3892068/149‑1 (MQ=255)
                                                                                                                                            ggTACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTGGCGCTGAAGGTGAGTTGTGCGTTAGCGTCGGCGATAAAGTATTGCGCGGCCAGCCGCTTACCCGTGGTCGCGGCAAAATGCTGCCTGTTCACGCGCCCACCt          <  1:3950833/149‑1 (MQ=255)
                                                                                                                                             gTACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTGGCGCTGAAGGTGAGTTGTGCGTTAGCGTCGGCGATAAAGTATTGCGCGGCCAGCCGCTTACCCGTGGTCGCGGCAAAATGCTGCCTGTTCACGCGCCCACCTc         <  1:3170429/149‑1 (MQ=255)
                                                                                                                                              tACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTGGCGCTGAAGGTGAGTTGTGCGTTAGCGTCGGCGATAAAGTATTGCGCGGCCAGCCGCTTACCCGTGGTCGCGGCAAAATGCTGCCTGTTCACGCGCCCACCTCg        <  2:3612253/149‑1 (MQ=255)
                                                                                                                                               aCCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTGGCGCTGAAGGTGAGTTGTGCGTTAGCGTCGGCGATAAAGTATTGCGCGGCCAGCCGCTTACCCGTGGTCGCGGCAAAATGCTGCCTGTTCACGCGCCCACCTCgg       >  1:5195215/1‑149 (MQ=255)
                                                                                                                                               aCCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTGGCGCTGAAGGTGAGTTGTGCGTTAGCGTCGGCGATAAAGTATTGCGCGGCCAGCCGCTTACCCGTGGTCGCGGCAAAATGCTGCCTGTTCACGCGCCCACCTCgg       >  1:2972003/1‑149 (MQ=255)
                                                                                                                                                ccccTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTGGCGCTGAAGGTGAGTTGTGCGTTAGCGTCGGCGATAAAGTATTGCGCGGCCAGCCGCTTACCCGTGGTCGCGGCAAAATGCTGCCTGTTCACGCGCCCACCTCggg      <  2:432096/149‑1 (MQ=255)
                                                                                                                                                ccccTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTGGCGCTGAAGGTGAGTTGTGCGTTAGCGTCGGCGATAAAGTATTGCGCGGCCAGCCGCTTACCCGTGGTCGCGGCAAAATGCTGCCTGTTCACGCGCCCACCTCggg      <  1:1558060/149‑1 (MQ=255)
                                                                                                                                                  ccTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTGGCGCTGAAGGTGAGTTGTGCGTTAGCGTCGGCGATAAAGTATTGCGCGGCCAGCCGCTTACCCGTGGTCGCGGCAAAATGCTGCCTGTTCACGCGCCCACCTCGGGTa    <  1:1137792/149‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
CCATTCCCGTGCGTATCATTCCCGTGGAACACCATGCTTAAGTTATTCTCTGCATTCAGAAAAAATAAAATCTGGGATTTCAACGGCGGCATCCATCCACCGGAGATGAAAACCCAGTCCAACGGTACACCCCTGCGCCAGGTACCCCTGGCGCAGCGTTTTGTTATTCCACTGAAACAGCATATTGGCGCTGAAGGTGAGTTGTGCGTTAGCGTCGGCGATAAAGTATTGCGCGGCCAGCCGCTTACCCGTGGTCGCGGCAAAATGCTGCCTGTTCACGCGCCCACCTCGGGTACC  >  CP009273/1701143‑1701439

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑